CN102718603A - Compound microbial fertilizer and preparation method thereof - Google Patents
Compound microbial fertilizer and preparation method thereof Download PDFInfo
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- CN102718603A CN102718603A CN2012102411131A CN201210241113A CN102718603A CN 102718603 A CN102718603 A CN 102718603A CN 2012102411131 A CN2012102411131 A CN 2012102411131A CN 201210241113 A CN201210241113 A CN 201210241113A CN 102718603 A CN102718603 A CN 102718603A
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Abstract
The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.
Description
Technical field
The present invention relates to a kind of composite microbiological fertilizer and preparation method thereof, belong to the microbial fertilizer technical field.
Technical background
China is a large agricultural country, and fertilizer occupies important status in China's grain-production.Yet, in the use of fertilizer, there are some irrational factors, make soil fertility descend, utilization rate of fertilizer reduces; Soil structure is destroyed, and causes soil compaction, preserve moisture and fertility ability drop, has had a strong impact on output and the quality of farm crop.
Along with China's Agricultural Development, the quantity discharged of tangerine stalk, ight soil the like waste also improves thereupon, and these wastes can not get good use; The crop straw burning phenomenon is growing on and on; A large amount of poultry, poultry manures are abandoned, and become serious source of pollution, solve the outlet of these organic wastes; The protection environment is current urgent problem.In addition, the farm crop normal growth not only needs the various nutrients of sufficient amount, and various nutrient will have suitable ratio, has ignored any one aspect, all can cause the crop nutrition content imbalance.Therefore a kind of comprehensive nutrition is provided, the suitable fertilizer of each nutrient is present urgent problem.
Chinese patent document CN101928186A (application number 201010115928.6) discloses a kind of composite microbiological fertilizer of controlling crop diseases and insect pests, contains complex micro organism fungicide, is cooperated by mass parts by following raw material to form; 10～35 parts of composite microbiological fertilizer microbial inoculums; 3～15 parts of complex microorganism agricultural chemicals microbial inoculums; 1～5 part of insecticidal synergist; 1～5 part of plant-growth regulator; 20～60 parts of the peats composed of rotten mosses; 5～15 parts of expanded chicken manures; 5～50 parts in mineral manure; 1～3 part in tackiness agent; Said composite microbiological fertilizer microbial inoculum is formulated by bacillus megaterium and bacillusmusilaginosiengineering; Said complex microorganism agricultural chemicals microbial inoculum, is formed with peat composed of rotten mosses absorption after adding the solid-state oxygenation agent of SPC-D type mixed fermentation again by mixing by bacillus thuringiensis and subtilis.Above-mentioned fertilizer makes its effect with diseases prevention worm through adding microbial pesticide, but fertilizer efficiency and reclamation result are general.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of composite microbiological fertilizer and preparation method thereof and application are provided.
A kind of composite microbiological fertilizer is made by following raw materials mix, is parts by weight:
200 ~ 500 parts of yeast fermentation granulated slags, 200 ~ 500 parts of fertilizers, 50 ~ 300 parts in urea, K
2SO
450 ~ 200 parts, NH
4H
2PO
450 ~ 200 parts, 30 ~ 50 parts of clays, 10 ~ 20 parts of complex micro organism fungicides;
Described complex micro organism fungicide is by bacillusmusilaginosiengineering (Bacillus mucilaginosus) CICC23640; Bacillus megaterium (Bacillus megaterium) CICC21694; Bacillus cereus (Bacillus cereus) CICC21687; Bacillus thuringiensis (Bacillus thuringiensis) CICC20565; Blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104; Pichia farinose bacterium (Pichia farinosa) ACCC20025; Streptomyces microflavus (Streptomyces microflavus) ACCC41134 makes through fermentation.
Preferred according to the present invention, the every gram of above-mentioned complex micro organism fungicide contains: bacillusmusilaginosiengineering (Bacillus mucilaginosus) CICC23640 viable count 6～18 * 10
8Individual, bacillus megaterium (Bacillus megaterium) CICC21694 viable count 5～15 * 10
8Individual, bacillus cereus (Bacillus cereus) CICC21687 viable count 6～18 * 10
8Individual, bacillus thuringiensis (Bacillus thuringiensis) CICC20565 viable count 5～15 * 10
8Individual, blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104 viable count 3～9 * 10
8Individual, pichia farinose bacterium (Pichia farinosa) ACCC20025 viable count 1～3 * 10
8Individual, streptomyces microflavus (Streptomyces microflavus) ACCC41134 spore count 3～9 * 10
8Individual.
Preferred based on the present invention, above-mentioned fertilizer can carry out compost fermentation by prior art with animal wastes and crop material, also can prepare as follows:
The crop material powder of 20 ~ 40 weight parts and the animal excrement of 60 ~ 80 weight parts are mixed, regulate pH6.0 ~ 7.0, add water and regulate water ratio to 40～60wt%, compost when temperature reaches 50 ~ 70 ℃ in waiting to pile, fermented 20 ~ 40 days, dried then, pulverized, and promptly got.
Above-mentioned streptomyces microflavus (Streptomyces microflavus) ACCC41134, pichia farinose bacterium (Pichia farinosa) ACCC20025 and blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104 are all available from Chinese agriculture microbial strains preservation administrative center.
Above-mentioned bacillusmusilaginosiengineering (Bacillus mucilaginosus) CICC23640, bacillus megaterium (Bacillus megaterium) CICC21694, bacillus cereus (Bacillus cereus) CICC21687 and bacillus thuringiensis (Bacillus thuringiensis) CICC20565 are all available from Chinese industrial microbial strains preservation administrative center.
The yeast fermentation granulated slag is a remaining residue during yeast is produced, and can buy through market.Preferably, organic content 45-55wt% in the yeast fermentation granulated slag, potassium content 8-15wt%.
The preparation method of above-mentioned composite microbiological fertilizer, step is following:
(1) bacillusmusilaginosiengineering CICC23640, bacillus megaterium CICC21694, bacillus cereus CICC21687 and bacillus thuringiensis CICC20565 are inoculated in liquid seed culture medium respectively; Equal liquid culture 18～24h under 30～37 ℃ of conditions, the inoculum size by 10 ~ 20wt% is inoculated in the liquid fermentation medium then, cultivates 3 ~ 5 days at 30～37 ℃; Adsorb with the peat composed of rotten mosses; 35 ~ 45 ℃ of oven dry are pulverized, and sieve;
Make respectively and contain bacillusmusilaginosiengineering CICC23640 viable count 3～8 * 10
9The microbial inoculum A of individual/g, contain bacillus megaterium CICC21694 viable count 3～8 * 10
9The microbial inoculum B of individual/g, bacillus cereus CICC21687 viable count 3～8 * 10
9The microbial inoculum C of individual/g and bacillus thuringiensis CICC20565 viable count 3～8 * 10
9The microbial inoculum D of individual/g;
(2) blown-ball Azotobacter ACCC11104 is inoculated in the liquid seed culture medium, liquid culture 18～24h under 25～30 ℃ of conditions, the inoculum size by 10 ~ 20wt% is inoculated in blown-ball Azotobacter in the liquid fermentation medium then; Cultivated 3 ~ 5 days at 25～30 ℃; With peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized; Sieve, make and contain blown-ball Azotobacter ACCC11104 viable count 1～5 * 10
9The microbial inoculum E of individual/g;
(3) pichia farinose bacterium ACCC20025 is inoculated in the liquid seed culture medium, 25 ~ 30 ℃ of conditions under liquid culture 48 ~ 72h, the inoculum size by 10 ~ 20wt% is inoculated in the pichia farinose bacterium in the liquid fermentation medium then; Cultivated 3 ~ 5 days at 25～30 ℃; With peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized; Sieve, make and contain pichia farinose bacterium ACCC20025 viable count 1～5 * 10
9The microbial inoculum F of individual/g;
(4) streptomyces microflavus ACCC41134 is inoculated in solid medium, under 25～30 ℃ of conditions, cultivates 48～72h, add sterilized water adjustment spore concentration to 10
8Individual/mL, the inoculum size by 10 ~ 20wt% is inoculated in the liquid fermentation medium then, cultivates 5～7 days at 25～30 ℃, and with peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized, and sieve, and make and contain streptomyces microflavus ACCC41134 spore count 1～5 * 10
9The microbial inoculum G of individual/g;
(5) the microbial inoculum A that step (1)～(4) is made, microbial inoculum B, microbial inoculum C, microbial inoculum D, microbial inoculum E, microbial inoculum F and microbial inoculum G make complex micro organism fungicide by weight the mixed of 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3;
(6) with yeast fermentation granulated slag, fertilizer, urea, K
2SO
4, NH
4H
2PO
4, the complex micro organism fungicide proportional mixing that makes of clay and step (5), make composite microbiological fertilizer.
Preferred according to the present invention, the liquid seed culture medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Yeast extract paste 5g, peptone 10g, NaCl10g, adding distil water be to 1000mL, pH7.0 ~ 8.0;
Preferred according to the present invention, the liquid fermentation medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Semen Maydis powder 15 ~ 25g, soya-bean milk 30 ~ 40mL, glucose 3 ~ 8g, (NH
4)
2SO
43 ~ 7g, KH
2PO
40.1 ~ 0.5g, MnSO
40.1 ~ 0.3g, MgSO
47H
2O 0.1 ~ 0.3g adds water to 800～1000mL, pH7.0 ~ 8.0.
Preferred according to the present invention, the liquid seed culture medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Glucose 20g, K
2HPO
41g, MgSO
47H
2O 0.2g, FeSO
47H
2O 0.25g, Na
2MoO
42H
2O 0.5g, CaCO
30.5g, add water to 1000mL, pH7.0 ~ 7.5.
Preferred according to the present invention, the liquid fermentation medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Sucrose 15 ~ 20g, MgSO
47H
2O 0.2 ~ 0.5g, K
2HPO
40.2 ~ 0.5g, NaCl 0.2 ~ 0.5g, CaCl
22H
2O0.05 ~ 0.1g, yeast extract paste 2 ~ 5g, 2 ~ 3 of liquid microelements add water to 800～1000mL, pH7.0 ~ 7.5;
Above-mentioned micro-fluid component is following:
Na
2MoO
42H
2O 5g, H
3BO
35g, zero(ppm) water is settled to 1000mL.
Preferred according to the present invention, the liquid seed culture medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
5 ° of B é wort 1000mL;
Preferred according to the present invention, the liquid fermentation medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
Sucrose 30 ~ 50g, Zulkovsky starch 1 ~ 3g, yeast powder 30 ~ 50g, peptone 8 ~ 15g, NaCl 3 ~ 8g, pH6.5 ~ 7.0.
Preferred according to the present invention, the solid medium in the said step (4) adopts following raw materials mix, after sterilization, makes:
Potato juice 1000mL, glucose 20g, agar 20g;
Preferred according to the present invention, the liquid fermentation medium in the said step (4) adopts following raw materials mix, after sterilization, makes:
Cottonseed cake 20 ~ 40g, Semen Maydis powder 10 ~ 15g, Zulkovsky starch 8～12g, sucrose 3 ~ 5g, (NH
4)
2SO
41 ~ 2g, NaCl2 ~ 4g, CaCO
33 ~ 5 grams, K
2HPO
40.3 ~ 0.5g adds water to 800 ~ 1000mL, pH7.0 ~ 8.0.
Organic content reaches 20 ~ 40% in the composite microbiological fertilizer according to the invention, and N, P, K total content reach 6 ~ 25%, and living bacteria count reaches 0.3 ~ 0.9 hundred million/g.
Product of the present invention can be used as base fertilizer, and amount of application is 50 ~ 100kg/ mu, in being manured into soil during whole ground.
Beneficial effect
1, the present invention in fertilizer, added have fixed nitrogen, the multiple mikrobe of phosphorus decomposing, potassium decomposing effect, can increase the nitrogen content of soil, improved the utilization ratio of chemical fertilizer, help the structure of improving the soil, improve crop yield and quality; Simultaneously, the mikrobe in the fertilizer can produce multiple meta-bolites, and these meta-bolitess can effectively disease-resistant pest-resistant evil;
2, the present invention adopts the culture propagation granulated slag as raw material; The culture propagation granulated slag is the residue behind the culture propagation; The content of organic matter reaches more than 50%; Potassium content reaches more than 10%; Also contain nutritions such as nitrogen, amino acid, vitamin, mycoprotein in addition; It is added in the fertilizer, be fertilizer rich nutrient contents is provided, realized the reasonable utilization of discarded object again; Also add animal wastes and straw simultaneously, help the equilibrium of nutritional labeling, increase soil fertility;
3, desinsection, fertilising are once accomplished, and have promptly reduced the generation of insect pest, have improved the quality and yield of crop again;
4, preparation technology of the present invention is simple, and production process does not need main equipment, and production cost is low, and environmental pollution is low.
The practical implementation method
Below in conjunction with embodiment the present invention is done further elaboration, but institute of the present invention protection domain is not limited thereto.
Streptomyces microflavus described in the embodiment (Streptomyces microflavus) ACCC41134, pichia farinose bacterium (Pichia farinosa) ACCC20025 and blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104 are all available from Chinese agriculture microbial strains preservation administrative center.
Bacillusmusilaginosiengineering described in the embodiment (Bacillus mucilaginosus) CICC23640, bacillus megaterium (Bacillus megaterium) CICC21694, bacillus cereus (Bacillus cereus) CICC21687 and bacillus thuringiensis (Bacillus thuringiensis) CICC20565 are all available from Chinese industrial microbial strains preservation administrative center.
The granulated slag of yeast fermentation described in the embodiment is available from Angel Yeast company, the about 50wt% of organic content, the about 10wt% of potassium content.
The raw material of substratum described in the embodiment is commercially available prod commonly used, this area, and compound method is by the state of the art preparation; Used other chemical reagent are commercially available prod commonly used among the embodiment.
Fertilizer prepares as follows described in the embodiment:
The crop material powder of 30 weight parts and the swine excrement of 70 weight parts are mixed, regulate pH7.0, add water and regulate water ratio to 60wt%, compost when temperature reaches 60 ℃ in waiting to pile, fermented 30 days, dried, is crushed to 2mm then, promptly got.
Embodiment 1
A kind of preparation method of composite microbiological fertilizer, step is following:
(1) bacillusmusilaginosiengineering CICC23640, bacillus megaterium CICC21694, bacillus cereus CICC21687 and bacillus thuringiensis CICC20565 are inoculated in liquid seed culture medium respectively; Liquid culture 24h under 30 ℃ of conditions, the inoculum size by 10wt% is inoculated in the liquid fermentation medium then, cultivates 3 days at 30 ℃; Adsorb with the peat composed of rotten mosses; 45 ℃ of oven dry are pulverized, and sieve;
Make respectively and contain bacillusmusilaginosiengineering CICC23640 viable count 6 * 10
9The microbial inoculum A of individual/g, contain bacillus megaterium CICC21694 viable count 5 * 10
9The microbial inoculum B of individual/g, bacillus cereus CICC21687 viable count 6 * 10
9The microbial inoculum C of individual/g and bacillus thuringiensis CICC20565 viable count 5 * 10
9The microbial inoculum D of individual/g;
Liquid seed culture medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Yeast extract paste 5g, peptone 10g, NaCl10g, adding distil water be to 1000mL, pH7.0;
Liquid fermentation medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Semen Maydis powder 20g, soya-bean milk 40mL, glucose 5g, (NH
4)
2SO
45g, KH
2PO
40.3g, MnSO
40.2g, MgSO
47H
2O 0.2g adds water to 1000mL, pH7.0.
(2) blown-ball Azotobacter ACCC11104 is inoculated in the liquid seed culture medium, liquid culture 24h under 28 ℃ of conditions, the inoculum size by 10wt% is inoculated in blown-ball Azotobacter in the liquid fermentation medium then; Cultivated 3 days at 28 ℃; With peat composed of rotten mosses absorption, 45 ℃ of oven dry are pulverized; Sieve, make and contain blown-ball Azotobacter ACCC11104 viable count 3 * 10
9The microbial inoculum E of individual/g;
Preferred according to the present invention, the liquid seed culture medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Glucose 20g, K
2HPO
41g, MgSO
47H
2O 0.2g, FeSO
47H
2O 0.25g, Na
2MoO
42H
2O 0.5g, CaCO
30.5g, add water to 1000mL, pH7.2.
Preferred according to the present invention, the liquid fermentation medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Sucrose 15g, MgSO
47H
2O 0.2g, K
2HPO
40.2g, NaCl 0.2g, CaCl
22H
2O 0.05g, yeast extract paste 2g, 2 of liquid microelements add water to 1000mL, pH7.2;
Above-mentioned micro-fluid component is following:
Na
2MoO
42H
2O 5g, H
3BO
35g, zero(ppm) water is settled to 1000mL.
(3) pichia farinose bacterium ACCC20025 is inoculated in the liquid seed culture medium, 28 ℃ of conditions under liquid culture 48h, the inoculum size by 15wt% is inoculated in the pichia farinose bacterium in the liquid fermentation medium then; Cultivated 4 days at 30 ℃; With peat composed of rotten mosses absorption, 45 ℃ of oven dry are pulverized; Sieve, make and contain pichia farinose bacterium ACCC20025 viable count 10
9The microbial inoculum F of individual/g;
Preferred according to the present invention, the liquid seed culture medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
5 ° of B é wort 1000mL;
Preferred according to the present invention, the liquid fermentation medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
Sucrose 40g, Zulkovsky starch 2g, yeast powder 40g, peptone 10g, NaCl5g, pH7.0.
(4) streptomyces microflavus ACCC41134 is inoculated in solid medium, under 28 ℃ of conditions, cultivates 72h, add sterilized water adjustment spore concentration to 10
8Individual/mL, the inoculum size by 15wt% is inoculated in the liquid fermentation medium then, cultivates 7 days at 28 ℃, and with peat composed of rotten mosses absorption, 45 ℃ of oven dry are pulverized, and sieve.Make and contain streptomyces microflavus ACCC41134 spore count 3 * 10
9The microbial inoculum G of individual/g.
Preferred according to the present invention, the liquid fermentation medium in the said step (4) adopts following raw materials mix, after sterilization, makes:
Cottonseed cake 30g, Semen Maydis powder 10g, Zulkovsky starch 10g, sucrose 5g, (NH
4)
2SO
41g, NaCl 3g, CaCO
35 grams, K
2HPO
40.4g, add water to 1000mL, pH7.0.
(5) the microbial inoculum A that step (1) ~ (4) is made, microbial inoculum B, microbial inoculum C, microbial inoculum D, microbial inoculum E, microbial inoculum F and microbial inoculum G make complex micro organism fungicide by weight the mixed of 1 ﹕, 1 ﹕, 1 ﹕, 2 ﹕, 2 ﹕, 2 ﹕ 1, and total count is 3.8 * 10
9Individual/g;
(6) with yeast fermentation granulated slag 250kg, fertilizer 250kg, urea 250kg, K
2SO
4100kg, NH
4H
2PO
4100kg, clay 50kg mix with the complex micro organism fungicide 20kg that step (4) makes, and the compound batch mixing that mixes is arrived the tablets press granulation through conveyor delivery, and oven drying at low temperature promptly makes composite microbiological fertilizer.
Organic content is greater than 20wt% in the gained composite microbiological fertilizer, and N, P, K total content are greater than 25wt%, and living bacteria count is greater than 0.7 hundred million/g.
Embodiment 2
Like embodiment 1 described preparation method; Difference is, in the step (5), with the microbial inoculum A that makes, microbial inoculum B, microbial inoculum C, microbial inoculum D, microbial inoculum E, microbial inoculum F and the microbial inoculum G mixed by weight 1 ﹕, 1 ﹕, 2 ﹕, 3 ﹕, 1 ﹕, 1 ﹕ 1; Make complex micro organism fungicide, total count is 4.5 * 10
9Individual/g.
Embodiment 3
With yeast fermentation granulated slag 340kg, fertilizer 200kg, urea 200kg, K
2SO
4100kg, NH
4H
2PO
4The complex micro organism fungicide 15kg that makes among 110kg, clay 50kg and the embodiment 2 mixes, with the compound batch mixing that mixes through conveyor delivery to the tablets press granulation, oven drying at low temperature promptly makes composite microbiological fertilizer.
Gained fertilizer organic content 25wt%, N, P, K total content are greater than 25wt%, and living bacteria count is greater than 0.6 hundred million/g.
Embodiment 4
Like embodiment 1 described preparation method, difference is, in the step (5), with yeast fermentation granulated slag 250kg, fertilizer 320kg, urea 200kg, K
2SO
4100kg, NH
4H
2PO
480kg, clay 50kg mix with the complex micro organism fungicide 15kg that step (4) makes, and the compound batch mixing that mixes is arrived the tablets press granulation through conveyor delivery, and oven drying at low temperature promptly makes composite microbiological fertilizer.
Gained fertilizer organic content is greater than 25wt%, and N, P, K total content are greater than 20wt%, and effectively viable bacteria is greater than 0.5 hundred million/g.
Embodiment 5
The application of composite microbiological fertilizer:
Adopt randomized blocks, sub-district area 100m
2, establish 3 repetitions, use 50kg/ mu with the composite microbiological fertilizer among the embodiment 3 as base fertilizer with embodiment 1.Be respectively treatment group 1, treatment group 2.
Fertilizer to use embodiment 1 among the patent CN101928186A is used 50kg/ mu as base fertilizer, is treatment group 3.
With applying fertilizer district not as the check plot.
Adopt the checkerboard type sampling method, put down in writing every sub-district insect pest strain number, calculate control effect by following formula:
Control effect (%)=(check plot insect pest strain number-treatment zone insect pest strain number)/check plot insect pest strain number * 100%
Above-mentioned group is removed the difference that applies fertilizer to the subsoil, and manuring late and management are all identical.
Detected result:
Table 1 different treatment is to the influence of Chinese cabbage output
| Handle | Contrast | Handle 1 | Handle 2 | Handle 3 |
| Mean yield (kg/ mu) | 4325 | 5225 | 5244 | 4950 |
| Raising the output (%) | 20.8% | 21.2% | 14.5% |
Table 2 different treatment is to the influence of pest control effect
| Handle | Handle 1 | Handle 2 | Handle 3 |
| Control effect (%) | 66% | 64% | 58% |
Interpretation of result:
By finding out in table 1 and the table 2, use compound manure the growth of Chinese cabbage is had significant promoter action, can significantly improve the output of Chinese cabbage, reduce the generation of insect pest.Wherein, no matter use composite microbiological fertilizer of the present invention is on the control effect to the raising of output and insect pest, all to be higher than fertilizer described in the embodiment 1 among the patent CN101928186A.The output raising the output of composite microbiological fertilizer of the present invention reaches more than 20%; And fertilizer units increased in production described in the embodiment 1 has only 14.5% among the CN101928186A; The control effect of the insect pest of composite microbiological fertilizer of the present invention is about 65%, and the control effect of fertilizer described in the embodiment 1 has only 58% among the CN101928186A.Explain that composite microbiological fertilizer action effect of the present invention is superior to fertilizer described in the CN101928186A1.
Claims (8)
1. a composite microbiological fertilizer is characterized in that, is made by following raw materials mix, is parts by weight:
200 ~ 500 parts of yeast fermentation granulated slags, 200 ~ 500 parts of fertilizers, 50 ~ 300 parts in urea, K
2SO
450 ~ 200 parts, NH
4H
2PO
450 ~ 200 parts, 30 ~ 50 parts of clays, 10 ~ 20 parts of complex micro organism fungicides;
Described complex micro organism fungicide is by bacillusmusilaginosiengineering (Bacillus mucilaginosus) CICC23640; Bacillus megaterium (Bacillus megaterium) CICC21694; Bacillus cereus (Bacillus cereus) CICC21687; Bacillus thuringiensis (Bacillus thuringiensis) CICC20565; Blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104; Pichia farinose bacterium (Pichia farinosa) ACCC20025; Streptomyces microflavus (Streptomyces microflavus) ACCC41134 makes through fermentation.
2. composite microbiological fertilizer as claimed in claim 1 is characterized in that, the every gram of said complex micro organism fungicide contains: bacillusmusilaginosiengineering (Bacillus mucilaginosus) CICC23640 viable count 6～18 * 10
8Individual, bacillus megaterium (Bacillus megaterium) CICC21694 viable count 5～15 * 10
8Individual, bacillus cereus (Bacillus cereus) CICC21687 viable count 6～18 * 10
8Individual, bacillus thuringiensis (Bacillus thuringiensis) CICC20565 viable count 5～15 * 10
8Individual, blown-ball Azotobacter (Azotobacter chroococcum) ACCC11104 viable count 3～9 * 10
8Individual, pichia farinose bacterium (Pichia farinosa) ACCC20025 viable count 1～3 * 10
8Individual, streptomyces microflavus (Streptomyces microflavus) ACCC41134 spore count 3～9 * 10
8Individual.
3. composite microbiological fertilizer as claimed in claim 1 is characterized in that, said fertilizer prepares as follows:
The crop material powder of 20 ~ 40 weight parts and the animal excrement of 60 ~ 80 weight parts are mixed, regulate pH6.0 ~ 7.0, add water and regulate water ratio to 40～60wt%, compost when temperature reaches 50 ~ 70 ℃ in waiting to pile, fermented 20 ~ 40 days, dried then, pulverized, and promptly got.
4. the preparation method of the said composite microbiological fertilizer of claim 1, step is following:
(1) bacillusmusilaginosiengineering CICC23640, bacillus megaterium CICC21694, bacillus cereus CICC21687 and bacillus thuringiensis CICC20565 are inoculated in liquid seed culture medium respectively; Equal liquid culture 18～24h under 30～37 ℃ of conditions, the inoculum size by 10 ~ 20wt% is inoculated in the liquid fermentation medium then, cultivates 3 ~ 5 days at 30～37 ℃; Adsorb with the peat composed of rotten mosses; 35 ~ 45 ℃ of oven dry are pulverized, and sieve;
Make respectively and contain bacillusmusilaginosiengineering CICC23640 viable count 3～8 * 10
9The microbial inoculum A of individual/g, contain bacillus megaterium CICC21694 viable count 3～8 * 10
9The microbial inoculum B of individual/g, bacillus cereus CICC21687 viable count 3～8 * 10
9The microbial inoculum C of individual/g and bacillus thuringiensis CICC20565 viable count 3～8 * 10
9The microbial inoculum D of individual/g;
(2) blown-ball Azotobacter ACCC11104 is inoculated in the liquid seed culture medium, liquid culture 18～24h under 25～30 ℃ of conditions, the inoculum size by 10 ~ 20wt% is inoculated in blown-ball Azotobacter in the liquid fermentation medium then; Cultivated 3 ~ 5 days at 25～30 ℃; With peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized; Sieve, make the microbial inoculum E that contains 1～5 * 109/g of blown-ball Azotobacter ACCC11104 viable count;
(3) pichia farinose bacterium ACCC20025 is inoculated in the liquid seed culture medium, 25 ~ 30 ℃ of conditions under liquid culture 48 ~ 72h, the inoculum size by 10 ~ 20wt% is inoculated in the pichia farinose bacterium in the liquid fermentation medium then; Cultivated 3 ~ 5 days at 25～30 ℃; With peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized; Sieve, make and contain pichia farinose bacterium ACCC20025 viable count 1～5 * 10
9The microbial inoculum F of individual/g;
(4) streptomyces microflavus ACCC41134 is inoculated in solid medium, under 25～30 ℃ of conditions, cultivates 48～72h, add sterilized water adjustment spore concentration to 10
8Individual/mL, the inoculum size by 10 ~ 20wt% is inoculated in the liquid fermentation medium then, cultivates 5～7 days at 25～30 ℃, and with peat composed of rotten mosses absorption, 35 ~ 45 ℃ of oven dry are pulverized, and sieve, and make and contain streptomyces microflavus ACCC41134 spore count 1～5 * 10
9The microbial inoculum G of individual/g;
(5) the microbial inoculum A that step (1)～(4) is made, microbial inoculum B, microbial inoculum C, microbial inoculum D, microbial inoculum E, microbial inoculum F and microbial inoculum G make complex micro organism fungicide by weight the mixed of 1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3:1 ~ 3;
(6) with yeast fermentation granulated slag, fertilizer, urea, K
2SO
4, NH
4H
2PO
4, the complex micro organism fungicide proportional mixing that makes of clay and step (5), make composite microbiological fertilizer.
5. composite microbiological fertilizer as claimed in claim 1 is characterized in that, the liquid seed culture medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Yeast extract paste 5g, peptone 10g, NaCl10g, adding distil water be to 1000mL, pH7.0 ~ 8.0;
Liquid fermentation medium in the said step (1) adopts following raw materials mix, after sterilization, makes:
Semen Maydis powder 15 ~ 25g, soya-bean milk 30 ~ 40mL, glucose 3 ~ 8g, (NH
4)
2SO
43 ~ 7g, KH
2PO
40.1 ~ 0.5g, MnSO
40.1 ~ 0.3g, MgSO
47H
2O0.1 ~ 0.3g adds water to 800 ~ 1000mL, pH7.0 ~ 8.0.
6. composite microbiological fertilizer as claimed in claim 1 is characterized in that, the liquid seed culture medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Glucose 20g, K
2HPO
41g, MgSO
47H
2O0.2g, FeSO
47H
2O0.25g, Na
2MoO
42H
2O0.5g, CaCO
30.5g, add water to 1000mL, pH7.0 ~ 7.5;
Liquid fermentation medium in the said step (2) adopts following raw materials mix, after sterilization, makes:
Sucrose 15 ~ 20g, MgSO
47H
2O0.2 ~ 0.5g, K
2HPO
40.2 ~ 0.5g, NaCl0.2 ~ 0.5g, CaCl
22H
2O0.05 ~ 0.1g, yeast extract paste 2 ~ 5g, 2 ~ 3 of liquid microelements add water to 800 ~ 1000mL, pH7.0 ~ 7.5;
Above-mentioned micro-fluid component is following:
Na
2MoO
42H
2O5g, H
3BO
35g, zero(ppm) water is settled to 1000mL.
7. composite microbiological fertilizer as claimed in claim 1 is characterized in that, the liquid seed culture medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
5 ° of B é wort 1000mL;
Liquid fermentation medium in the said step (3) adopts following raw materials mix, after sterilization, makes:
Sucrose 30 ~ 50g, Zulkovsky starch 1 ~ 3g, yeast powder 30 ~ 50g, peptone 8 ~ 15g, NaCl3 ~ 8g, pH6.5 ~ 7.0.
8. composite microbiological fertilizer as claimed in claim 1 is characterized in that, the solid medium in the said step (4) adopts following raw materials mix, after sterilization, makes:
Potato juice 1000mL, glucose 20g, agar 20g;
Liquid fermentation medium in the said step (4) adopts following raw materials mix, after sterilization, makes:
Cottonseed cake 20 ~ 40g, Semen Maydis powder 10 ~ 15g, Zulkovsky starch 8 ~ 12g, sucrose 3 ~ 5g, (NH
4)
2SO
41 ~ 2g, NaCl2 ~ 4g, CaCO
33 ~ 5 grams, K
2HPO
40.3 ~ 0.5g adds water to 800 ~ 1000mL, pH7.0 ~ 8.0.
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Address after: 253000 no.729, Hongyin Road, Dezhou Economic Development Zone, Dezhou City, Shandong Province Patentee after: Yuanhe Biotechnology (Dezhou) Co.,Ltd. Address before: 253000 Yuanhe agricultural company, No. 729, Hongyin Road, Decheng Economic Development Zone, De Zhou City, Shandong Province Patentee before: DEZHOU YUANHE AGRICULTURAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |