CN104609992B - A kind of salt-soda soil specific complex bacterial manure and preparation method thereof - Google Patents
A kind of salt-soda soil specific complex bacterial manure and preparation method thereof Download PDFInfo
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- CN104609992B CN104609992B CN201410844584.0A CN201410844584A CN104609992B CN 104609992 B CN104609992 B CN 104609992B CN 201410844584 A CN201410844584 A CN 201410844584A CN 104609992 B CN104609992 B CN 104609992B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The present invention relates to a kind of salt-soda soil specific complex bacterial manure and the preparation method of the composite bacterial fertilizer, belong to composite bacterial fertilizer technical field.The composite bacterial fertilizer, including the raw material of following percentage by weight is constituted：Dissolving phosphor and dissolving potassium class composite bacteria agent 10% 20%, kitchen garbage 40% 60%, diatomite 30% 40%.Wherein dissolving phosphor and dissolving potassium class composite bacteria agent is that mixed in equal amounts is obtained after bacillus megaterium, bacillus thuringiensis, Pseudomonas fluorescens, bacillus amyloliquefaciens, colloid bacillus cereus, azotobacter chroococcum are cultivated into centrifugation, freeze-drying respectively；Kitchen garbage is after sorted, removal of impurities and being dehydrated the kitchen garbage pulverizing of oil removing, to add composite bacteria agent and mixing enzyme preparation, is obtained after aerobic solid-state fermentation.The twice laid such as kitchen garbage and discarded diatomite can fundamentally be improved saline-alkali soil by the composite bacterial fertilizer.
Description
Technical field
The present invention relates to a kind of salt-soda soil specific complex bacterial manure, and it is related to the preparation method of the composite bacterial fertilizer, belongs to compound
Bacterial manure technical field.
Background technology
The salinization of soil is a world-famous puzzle.Saline-alkali soil is a kind of widely distributed soil types in earth land, about
Account for the 25% of the land gross area.Chinese saline-alkali soil has a very wide distribution, area is big, type is more, the hm of the gross area about 300,000,0002.According to estimates,
The saline-alkali soil in the whole world is annual with 100,000-15 ten thousand hm2Speed increase, our faces enormous challenges, rationally using saline and alkaline soil
Resource turns into an important problem.Increasingly in short supply with world's land resource, increasing can to the modified utilization in saline and alkaline soil
Effectively to alleviate the grain contradiction not enough with soil in short supply.It is more universal to the research of composite bacterial fertilizer at present but saline and alkaline to improving
The research on ground is actually rare.
The Chinese invention of application number 201410159509.0《Microbial compound bacterial fertilizer and preparation method thereof》Disclose one kind
Microbial compound bacterial fertilizer and preparation method thereof, microorganism belonging to genus bacterial manure technical field.It contains organic matter >=30%, and nitrogen, phosphorus, potassium is each >=
4%, while every gram of bacterial manure contains：Phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, basophilic bacterium and each 1.5-2.5 of Halophiles hundred million are thermophilic thin
Bacterium and each 2-3 of thermophilic actinomycete hundred million.The wherein microorganism comprising improved soil structure, thermophilic salt halophile and thermophilic micro-
It is biological.The microorganism of improved soil structure includes azotobacter and phosphorus decomposing potassium solubilizing bacteria, and hardened soil can be made (to be drawn by chemical fertilizer
Rise) become loose, increase soil organic matter content, improve soil texture；Thermophilic bacteria and thermophilic actinomycete, can divide in several days
Solution is become thoroughly decomposed stalk, becomes agricultural fertilizer, increases soil fertility；Thermophilic salt basophilic bacterium, can under salt affected soil environment survival and reproduction, it is right
There is unique effect in the acid-base value for improving salt-soda soil, salt-soda soil is turned waste into wealth, bacterial manure is turned into normal soil and salt affected soil
Dual-purpose bacterial manure.But the invention is only disclosed using characteristic bacterium such as phosphate solubilizing bacteria, potassium solubilizing bacteria, Thermophilic Bacterias, undisclosed specific strain.
Kitchen garbage is the sanitary waste that resident is formed during personal consumption, refers mainly to the skin of leftovers, leftovers, melon and fruit
Core, the root leaf of vegetables, discarded animal or plant tissue etc., rich in a large amount of organic matters, easily degenerate, if treatment is easy not in time
, there is processing cost higher in the problems such as producing stench, grow harmful organism, it is impossible to effectively utilize etc. defect.At present to this research
Also it is more.
The Chinese invention of application number 201110365606.1 is disclosed《A kind of food waste fermentation composite bacteria and its preparation side
Method》A kind of food waste fermentation composite bacteria and preparation method thereof is disclosed, preparation method is comprised the following steps：Respectively by green wood
The strain of mould, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae and bacillus licheniformis carries out High Density Cultivation, will
The various single bacteriums that High Density Cultivation is obtained are dehydrated, and are prepared into hypopus microbial dry powder, then according to Trichoderma viride 20~
45 parts, 10~15 parts of bacillus subtilis, 20~30 parts of bread mold, 5~10 parts of photosynthetic bacteria, 7~15 parts of saccharomyces cerevisiae and ground
The weight ratio that 10~15 parts of clothing bacillus, is mixed to get food waste fermentation composite bacteria.The present invention is for processing kitchen garbage
Ferment effect is good, deodorizing effect is notable, and the kitchen garbage after being processed using compound bacteria-fermented can be used as organic fertilizer, becomes
Waste be changed into values, and avoids the pollution to water source, soil and air, and the harm to human body generation.
The Chinese invention of application number 201310030482.0《A kind of method of kitchen garbage treatment》Disclose a kind of kitchen rubbish
Rubbish on-site treatment method, including：A. kitchen garbage is carried out into separation of solid and liquid；B. the solid refuse that will be isolated passes through in treatment storehouse
Biochemical treatment forms organic fertilizer masterbatch；C. the treatment formation organic fertilizer that becomes thoroughly decomposed is carried out to the organic fertilizer masterbatch；D. have to described
Machine fertilizer is sieved.Restaurant, Party and government offices, enterprises and institutions, universities and colleges are placed in by by food waste treatment equipment
The rubbish such as scene produce ground, and regularly or sporadically domestic organic food rubbish is processed before not rotting, and reduce rubbish
Rubbish intermediate links, carry out innoxious treatment in situ, and kitchen garbage is converted into organic fertilizer masterbatch, so as to realize from source to kitchen
It is controlled on rubbish, not only reduces the expense that the cost of transportation and landfill of rubbish are burned, and reduce rubbish fermentation
The peculiar smell of process is produced, and whole handling process is totally-enclosed.
The Chinese invention of application number 201310278764.2 is disclosed《A kind of processing method of fermentation of kitchen waste into fertilizer》
It is related to a kind of processing method of fermentation of kitchen waste into fertilizer, the treatment effect of kitchen garbage treater is mainly improved in terms of two
Really, with kitchen garbage fermenting and producing bio-feritlizer.One is the artificial addition acinetobacter calcoaceticus in kitchen garbage processing procedure
Acinetobact ercalcoaceticusstrainB40, addition reaches more than every gram of kitchen garbage 10CFU.Two is to use
The penetrating odor produced in composite adsorbing material absorption fermentation process.Composite adsorbing material proportioning is rice bran：Wood chip：Activated carbon
10-20：3-5：1.The bio-feritlizer produced using the method has oil content low, the small spy of production process moderate stimulation smell
Point.
Foregoing invention solve the problems, such as it is certain, but further investigation kitchen garbage fermentation after how to continue place
Reason.Kitchen garbage the like waste how is made full use of, kind of a specific complex bacterial manure with good reduction saline-alkali soil pH is produced, from
Saline-alkali soil is fundamentally improved, is problem to be solved by this invention.
The content of the invention
To solve the above problems, the present invention provides a kind of salt-soda soil specific complex bacterial manure, and provides the system of the composite bacterial fertilizer
Preparation Method.The specific complex bacterial manure is rich in composite bacteria agent, organic matter and diatomite, it is adaptable to alkali-affected soil, can be quickly high
Effect reduces the pH of saline-alkali soil, improves soil physico-chemical property, increases saline-alkali soil nutrient, resists plant pest, promotes plant growth.
The salt-soda soil specific complex bacterial manure includes that the raw material of following percentage by weight is constituted：Dissolving phosphor and dissolving potassium class composite bacteria agent
10%-20%, kitchen garbage 40%-60%, diatomite 30%-40%；
The diatomite can be：With diatomite as filter medium, the discarded diatomite after use；
Preferably, the diatomite be produce pulullan polysaccharide sheet frame it is degerming after filter residue.
The present invention also provides the preparation method of the salt-soda soil specific complex bacterial manure, comprises the following steps：
The first step, the preparation of dissolving phosphor and dissolving potassium class composite bacteria agent；
Second step, the preparation of kitchen garbage；
3rd step, diatomaceous preparation；
4th step, dissolving phosphor and dissolving potassium composite bacteria agent, kitchen garbage, diatomite are mixed according to aforementioned proportion, are carried out after mixing
Granulator granulation, obtains finished particle.
The first step is that (bacillus megaterium, bacillus thuringiensis, fluorescence are false by the strain of slant medium preservation
Monad, bacillus amyloliquefaciens, colloid bacillus cereus and azotobacter chroococcum) activation, then respectively take a ring and access seed training
Foster base carries out shaking table culture respectively, 37 DEG C, reach stationary phase, mixed in equal amounts after centrifugation freeze-drying after 220r/min cultures 18h.
Bacillus megaterium, bacillus thuringiensis, Pseudomonas fluorescens, bacillus amyloliquefaciens, colloid bacillus cereus
And azotobacter chroococcum this six plants of bacterium are all from University Of Science and Technology Of Tianjin's strain library.
The second step is by sorted, removal of impurities and is dehydrated the kitchen garbage pulverizing of oil removing, with the kitchen garbage after crushing
Quality meter, adds composite bacteria agent, the mixing enzyme preparation of 0.02-0.04% of 1-3%, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen
30-40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, mid-term fermentation temperature 60
DEG C, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 3-5%, adjustment moisture content to 50%-60% are added in fermentation material, while adding life
After element long, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen 30-40%, 20r/min, stage temperature control fermentation, prior fermentation temperature
It is 50 DEG C to spend, and ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, is done
It is dry and crush；
The auxin is B family vitamin；
The B family vitamin addition is the 0.5-1% of fermentation material weight；
The composite bacteria agent, by bacillus licheniformis, bacillus larvae, bafillus natto and bacillus subtilis group
Into；
The bacillus licheniformis, bacillus larvae, bafillus natto and bacillus subtilis mass ratio are
0.8-1.2：1.0-1.4：0.6-0.8：0.8-1.2.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：- 12 parts of proteinase 8, amylase 10-15 parts, fat
Enzyme 12-18 parts, pectase 5-10 parts, cellulase 10-20 parts.
Preferably, the diatomite preparation method is as follows：
Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 10-20 days, crushes 14 mesh sieves.
Beneficial effect：
The invention provides a kind of compound bacteria with microorganism fungus kind, kitchen garbage and diatomite reduction salt-soda soil pH
Fertilizer, rich in microorganism, organic matter and diatomite.After testing, its main component index is as follows：Kitchen garbage, the content of organic matter
90%, pH7.98；Diatomite, the content of organic matter 20.79%, pH5.21 are combined for composite bacterial fertilizer of the invention, make the present invention
The composite bacterial fertilizer content of organic matter it is high, the content of organic matter of soil can be greatly enhanced after, and can efficiently reduce saline and alkaline
The pH value on ground.The microorganism of species and rich content being capable of chesson, improvement soil granular structure, reduction soil in composite bacterial fertilizer
Earth salt content, produces organic acid, reduces pH, and can resist pest and disease damage.Wherein, huge gemma bar is added in composite bacterial fertilizer
Bacterium, bacillus thuringiensis and Pseudomonas fluorescens, with good acid producing ability, ability of dissolving potassium, Pseudomonas in addition
Bacterium has preventive and therapeutic effect to plurality of plant diseases；And add the colloid bacillus cereus with ability of dissolving potassium and with nitrogen fixation
Azotobacter chroococcum.Organic matter is added in salt affected soil, particularly applying kitchen garbage can increase soil nutrient, promote to plant
Thing grows；Soil fine particles can be cohered, good crumb structure is formed, improves soil physico-chemical property.Diatomite can play guarantor
Wet, loose soil property, extension fertilizer efficiency EDD, growth encourage crop growth effect.Diatomite is removed from pulullan polysaccharide sheet frame
Filter residue after bacterium, in neutrality, porosity is big, and absorption property is strong for pH.Not only twice laid, in energy-conserving and environment-protective, with component other
Raw material is cooperateed with, and more brings the effect outside accident.The purpose of the present invention is to develop a kind of with good drop using above-mentioned material
The specific complex bacterial manure of less salt alkaline earth pH, fundamentally improves saline-alkali soil, and the composite bacterial fertilizer makes full use of kitchen garbage and diatom
Native the like waste, takes full advantage of resource, reduces the pollution to environment.Therefore, by discarded object minimizing, innoxious, resource
Change, the road for walking a cleaning low-carbon (LC) is imperative.
Specific embodiment：
Embodiment 1
A kind of salt-soda soil specific complex bacterial manure, including the raw material of following percentage by weight is constituted：Dissolving phosphor and dissolving potassium class compound bacteria
Agent 15%, kitchen garbage 50%, diatomite 35%；
The dissolving phosphor and dissolving potassium class composite bacteria agent is prepared by the method for comprising the following steps：
By bacillus megaterium, bacillus thuringiensis, Pseudomonas fluorescens, bacillus amyloliquefaciens, colloid gemma bar
Bacterium and the actication of culture of azotobacter chroococcum slant medium preservation, then respectively take ring access seed culture medium and are shaken respectively
Bed culture, 37 DEG C, reach stationary phase after 220r/min cultures 18h, mixed in equal amounts after centrifugation, freeze-drying.
The kitchen garbage is prepared by the method for comprising the following steps：
Kitchen garbage is collected, then sorting, removal of impurities carry out water-oil separating, solid portion is crushed, with the quality after crushing
Meter, adds 2% composite bacteria agent, 0.03% mixing enzyme preparation, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen 35%, 20r/
Min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage
70 DEG C of fermentation temperature, ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 4%, adjustment moisture content to 55% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 35%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 0.8% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=1.0：1.2：0.7：0.8.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase；
The diatomite be produce pulullan polysaccharide sheet frame it is degerming after filter residue.
The preparation method of the salt-soda soil specific complex bacterial manure comprises the following steps：
Dissolving phosphor and dissolving potassium class composite bacteria agent, kitchen garbage, diatomite are mixed according to aforementioned proportion, comminutor is carried out after mixing
Granulation, obtains finished particle.
The dissolving phosphor and dissolving potassium class composite bacteria agent preparation method is as follows：
The bacillus thuringiensis of slant medium preservation, bacillus amyloliquefaciens, bacillus megaterium and fluorescence is false
Monad, colloid bacillus cereus and azotobacter chroococcum, respectively take a ring and access seed culture medium.
Condition of culture：Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium, Pseudomonas fluorescens and circle
Brown nitrogen-fixing bacteria, 500mL conventional bottles, seed culture medium liquid amount 100mL, colloid bacillus cereus, 500mL baffle plates bottle, seed culture
Base liquid amount 50mL, 37 DEG C, 200rpm, culture 18h reaches stationary phase, obtains seed liquor；
By above-mentioned seed liquor, centrifugal treating obtains thalline respectively, with mixed in equal amounts after frozen drying.
The slant medium composition is as follows：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens slant medium group
Turn into, g/L：Peptone 10, beef extract 3, sodium chloride 5,20, pH7.2-7.4,121 DEG C of agar, 20min sterilizings.
Colloid bacillus cereus slant medium is silicate bacteria culture medium, constitutes and is, g/L：Mannitol 10, two is hydrated sulphur
Sour calcium 0.1, potassium dihydrogen phosphate 0.2, calcium carbonate 5.0, bitter salt 0.2, sodium chloride 0.2,20, pH7.0,121 DEG C of agar,
20min sterilizes.
Azotobacter chroococcum slant medium is constituted, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, biphosphate
Potassium 0.2, bitter salt 0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5, pH
7.2,121 DEG C, 20min sterilizings.
The seed culture medium composition is as follows respectively：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens seed culture medium group
Turn into, g/L：Glucose 10, three hypophosphite monohydrate hydrogen dipotassiums 6, potassium dihydrogen phosphate 3, urea 1.5, dusty yeast 5, bitter salt
0.41, pH7.0,121 DEG C, 20min sterilizings.
Colloid bacillus cereus seed culture medium is constituted, g/L：Sucrose 10, three hypophosphite monohydrate hydrogen dipotassiums 5, dusty yeast 0.5, six
Ferric Chloride Hydrated 0.02, calcium carbonate 0.4,0.2, pH7.0,121 DEG C of bitter salt, 20min sterilizings.
Azotobacter chroococcum seed culture medium is constituted, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, potassium dihydrogen phosphate
0.2, bitter salt 0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5, pH 7.2,
121 DEG C, 20min sterilizings.
The kitchen garbage preparation method is as follows：
Kitchen garbage is collected, then sorting, removal of impurities carry out water-oil separating, solid portion is crushed, with the quality after crushing
Meter, adds 2% composite bacteria agent, 0.03% mixing enzyme preparation, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen 35%, 20r/
Min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage
70 DEG C of fermentation temperature, ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 4%, adjustment moisture content to 55% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 35%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 0.8% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=1.0：1.2：0.7：0.8.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase；
The diatomite preparation method is as follows：
Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 10 days, crushes 14 mesh sieves.
Embodiment 2
A kind of salt-soda soil specific complex bacterial manure, is made up of the raw material of following percentage by weight：Dissolving phosphor and dissolving potassium class composite bacteria agent
10%th, kitchen garbage 60%, diatomite 30%；
The diatomite is market products.
The dissolving phosphor and dissolving potassium class composite bacteria agent is with embodiment 1；
The kitchen garbage is prepared by the method for comprising the following steps：
In terms of the quality of kitchen garbage after crushing, 1% composite bacteria agent, 0.04% mixing enzyme preparation is added, enter to act charitably
Oxygen solid state fermentation, with respect to dissolved oxygen 30%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h,
60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 3%, adjustment moisture content to 50% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 30%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 0.5% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=0.8：1.4：0.6：1.2.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase；
The preparation method of the salt-soda soil specific complex bacterial manure comprises the following steps：
Dissolving phosphor and dissolving potassium class composite bacteria agent, kitchen garbage, diatomite are mixed according to aforementioned proportion, comminutor is carried out after mixing
Granulation, obtains finished particle.
The dissolving phosphor and dissolving potassium class composite bacteria agent preparation method is with embodiment 1；
The kitchen garbage preparation method is as follows：
In terms of the quality of kitchen garbage after crushing, 1% composite bacteria agent, 0.04% mixing enzyme preparation is added, enter to act charitably
Oxygen solid state fermentation, with respect to dissolved oxygen 30%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h,
60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 3%, adjustment moisture content to 50% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 30%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 0.5% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=0.8：1.4：0.6：1.2.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase.
Embodiment 3
A kind of salt-soda soil specific complex bacterial manure, including the raw material of following percentage by weight is constituted：Dissolving phosphor and dissolving potassium class compound bacteria
Agent 20%, kitchen garbage 40%, diatomite 40%；
The dissolving phosphor and dissolving potassium class composite bacteria agent is with embodiment 1；
The kitchen garbage is prepared by the method for comprising the following steps：
In terms of the quality after kitchen garbage pulverizing, 3% composite bacteria agent, 0.02% mixing enzyme preparation is added, enter to act charitably
Oxygen solid state fermentation, with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h,
60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 5%, adjustment moisture content to 60% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 1% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=1.2：1.4：0.8：1.2.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase；
The diatomite be produce pulullan polysaccharide sheet frame it is degerming after filter residue.
The preparation method of the salt-soda soil specific complex bacterial manure comprises the following steps：
Dissolving phosphor and dissolving potassium class composite bacteria agent, kitchen garbage, diatomite are mixed according to aforementioned proportion, comminutor is carried out after mixing
Granulation, obtains finished particle.
The dissolving phosphor and dissolving potassium class composite bacteria agent preparation method is with embodiment 1.
The kitchen garbage preparation method is as follows：
In terms of the quality after kitchen garbage pulverizing, 3% composite bacteria agent, 0.02% mixing enzyme preparation is added, enter to act charitably
Oxygen solid state fermentation, with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h,
60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 5%, adjustment moisture content to 60% are added in fermentation material after addition auxin, to be carried out
Aerobic solid-state fermentation, with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, fermentation
6h, 60 DEG C of mid-term fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 1% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto bud
Spore bacillus：Bacillus subtilis=1.2：1.4：0.8：1.2.
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, lipase 14
Part, 8 parts of pectase, 15 parts of cellulase；
The diatomite preparation method is as follows：
Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 20 days, crushes 14 mesh sieves.
Experimental example 1：
Tested with composite bacterial fertilizer prepared by embodiment 2, effect is as follows：
On the salt-soda soil of Tianjin huge port, every mu is applied 5000kg/hm2, with specific reference to the salinization and alkalization meal with wine of saline-alkali soil
Feelings increase and decrease, using a season after, according to corresponding GB determine, 0-8cm pH decreaseds 0.56, the content of organic matter improve
45%, bud ratio is relatively compareed and shifts to an earlier date and improve 9.5%.
Experimental example 2：
Tested with composite bacterial fertilizer prepared by embodiment 1, effect is as follows：
On the salt-soda soil of Tianjin huge port, every mu is applied 5000kg/hm2, with specific reference to the salinization and alkalization meal with wine of saline-alkali soil
Feelings increase and decrease, using a season after, according to corresponding GB determine, 0-8cm pH decreaseds 0.62, the content of organic matter improve
32%, bud ratio is relatively compareed and shifts to an earlier date and improve 10.3%.
Experimental example 3
Tested with composite bacterial fertilizer prepared by embodiment 3, effect is as follows：
On the salt-soda soil of Tianjin huge port, every mu is applied 5000kg/hm2, with specific reference to the salinization and alkalization meal with wine of saline-alkali soil
Feelings increase and decrease, using a season after, according to corresponding GB determine, 0-8cm pH decreaseds 0.68, the content of organic matter improve
25%, bud ratio is relatively compareed and shifts to an earlier date and improve 12%.
Claims (10)
1. a kind of raw material composition of salt-soda soil specific complex bacterial manure, including following percentage by weight：Dissolving phosphor and dissolving potassium class composite bacteria agent
10%-20%, kitchen garbage 40%-60%, diatomite 30%-40%；
The dissolving phosphor and dissolving potassium class composite bacteria agent is prepared by the method for comprising the following steps：
By bacillus megaterium, bacillus thuringiensis, Pseudomonas fluorescens, bacillus amyloliquefaciens, colloid bacillus cereus with
And the actication of culture of azotobacter chroococcum slant medium preservation, then respectively take ring access seed culture medium carries out shaking table training respectively
Support, 37 DEG C, reach stationary phase after 220r/min cultures 18h, mixed in equal amounts after centrifugation, freeze-drying.
2. salt-soda soil specific complex bacterial manure according to claim 1, it is characterised in that the kitchen garbage is by including following step
It is prepared by rapid method：
By sorted, removal of impurities and the kitchen garbage pulverizing of oil removing is dehydrated, in terms of the kitchen garbage quality after crushing, adds 1-3%
Composite bacteria agent, the mixing enzyme preparation of 0.02-0.04%, carry out aerobic solid-state fermentation, with respect to dissolved oxygen 30-40%, 20r/min,
Stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage fermentation
Temperature 70 C, ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 3-5%, adjustment moisture content to 50%-60% are added in fermentation material, while adding auxin
Afterwards, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen 30-40%, 20r/min, stage temperature control is fermented, and prior fermentation temperature is
50 DEG C, ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries simultaneously
Crush；
The auxin is B family vitamin, and addition is the 0.5-1% of fermentation material weight；
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：- 12 parts of proteinase 8, amylase 10-15 parts, lipase
12-18 parts, pectase 5-10 parts, cellulase 10-20 parts；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto gemma bar
Bacterium：Bacillus subtilis=0.8-1.2：1.0-1.4：0.6-0.8：0.8-1.2.
3. salt-soda soil specific complex bacterial manure according to claim 1, it is characterised in that the diatomite is made by the following method
It is standby：
Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 10-20 days, crushes 14 mesh sieves.
4. salt-soda soil specific complex bacterial manure according to claim 1, it is characterised in that the slant medium composition is respectively such as
Under：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens, g/L：Peptone 10, ox
Meat extract 3, sodium chloride 5,20, pH7.2-7.4,121 DEG C of agar, 20min sterilizings；
Colloid bacillus cereus, g/L：Mannitol 10, calcium sulphate dihydrate 0.1, potassium dihydrogen phosphate 0.2, calcium carbonate 5.0, seven are hydrated
Magnesium sulfate 0.2, sodium chloride 0.2,20, pH7.0,121 DEG C of agar, 20min sterilizings；
Azotobacter chroococcum, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, potassium dihydrogen phosphate 0.2, bitter salt
0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5,7.2,121 DEG C of pH, 20min sterilizing.
5. salt-soda soil specific complex bacterial manure according to claim 1, it is characterised in that the seed culture medium composition is respectively such as
Under：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens, g/L：Glucose 10, three
Hypophosphite monohydrate hydrogen dipotassium 6, potassium dihydrogen phosphate 3, urea 1.5, dusty yeast 5,0.41, pH7.0,121 DEG C of bitter salt,
20min sterilizes；
Colloid bacillus cereus, g/L：Sucrose 10, three hypophosphite monohydrate hydrogen dipotassiums 5, dusty yeast 0.5, ferric chloride hexahydrate 0.02, carbonic acid
Calcium 0.4,0.2, pH7.0,121 DEG C of bitter salt, 20min sterilizings；
Azotobacter chroococcum, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, potassium dihydrogen phosphate 0.2, bitter salt
0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5,7.2,121 DEG C of pH, 20min sterilizing.
6. the method for preparing the claim any salt-soda soil specific complex bacterial manure of 1-5, comprises the following steps：
Dissolving phosphor and dissolving potassium class composite bacteria agent, kitchen garbage, diatomite are mixed according to aforementioned proportion, comminutor is carried out after mixing and is made
Grain, obtains finished particle；
The dissolving phosphor and dissolving potassium class composite bacteria agent preparation method is as follows：
By the bacillus thuringiensis of slant medium preservation, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas
Bacterium, colloid bacillus cereus and azotobacter chroococcum, respectively take a ring and access seed culture medium；
Condition of culture：Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium, Pseudomonas fluorescens and circle are brown solid
Nitrogen bacterium, 500mL conventional bottles, seed culture medium liquid amount 100mL, colloid bacillus cereus, 500mL baffle plates bottle, seed culture medium dress
Liquid measure 50mL, 37 DEG C, 200rpm, culture 18h reaches stationary phase, obtains seed liquor；By above-mentioned seed liquor, centrifugal treating is obtained respectively
To thalline, mixed in equal amounts after frozen drying.
7. the preparation method of salt-soda soil specific complex bacterial manure according to claim 6, it is characterised in that
The slant medium composition is as follows respectively：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens slant medium are constituted,
g/L：Peptone 10, beef extract 3, sodium chloride 5,20, pH7.2-7.4,121 DEG C of agar, 20min sterilizings；
Colloid bacillus cereus slant medium is silicate bacteria culture medium, constitutes and is, g/L：Mannitol 10, calcium sulphate dihydrate
0.1st, potassium dihydrogen phosphate 0.2, calcium carbonate 5.0, bitter salt 0.2, sodium chloride 0.2,20, pH7.0,121 DEG C of agar,
20min sterilizes；
Azotobacter chroococcum slant medium is constituted, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, potassium dihydrogen phosphate
0.2, bitter salt 0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5, pH 7.2,
121 DEG C, 20min sterilizings；
The seed culture medium composition is as follows respectively：
Bacillus thuringiensis, bacillus amyloliquefaciens, bacillus megaterium and Pseudomonas fluorescens seed culture medium are constituted,
g/L：Glucose 10, three hypophosphite monohydrate hydrogen dipotassiums 6, potassium dihydrogen phosphate 3, urea 1.5, dusty yeast 5, bitter salt 0.41,
PH7.0,121 DEG C, 20min sterilizings；
Colloid bacillus cereus seed culture medium is constituted, g/L：Sucrose 10, three hypophosphite monohydrate hydrogen dipotassiums 5, dusty yeast 0.5, six are hydrated
Iron chloride 0.02, calcium carbonate 0.4,0.2, pH7.0,121 DEG C of bitter salt, 20min sterilizings；
Azotobacter chroococcum seed culture medium is constituted, g/L：Glucose 20, three hypophosphite monohydrate hydrogen dipotassiums 0.8, potassium dihydrogen phosphate 0.2,
Bitter salt 0.2, calcium carbonate 0.5, ferric chloride hexahydrate 0.125, sodium molybdate 0.25, ammonium nitrate 0.5, pH 7.2,121
DEG C, 20min sterilizings.
8. the preparation method of salt-soda soil specific complex bacterial manure according to claim 6, it is characterised in that the kitchen garbage system
Preparation Method is as follows：
Kitchen garbage is collected, then sorting, removal of impurities carry out water-oil separating, solid portion is crushed, in terms of the quality after crushing,
2% composite bacteria agent, 0.03% mixing enzyme preparation is added, aerobic solid-state fermentation is carried out, with respect to dissolved oxygen 35%, 20r/min, rank
Segmentation temperature control is fermented, and prior fermentation temperature is 50 DEG C, and ferment 6h, 60 DEG C of mid-term fermentation temperature, and ferment 1h, later stage fermentation temperature
70 DEG C of degree, ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 4%, adjustment moisture content to 55% are added in fermentation material after addition auxin, to be carried out aerobic
Solid state fermentation, with respect to dissolved oxygen 35%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, in
60 DEG C of phase fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 0.8% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto gemma bar
Bacterium：Bacillus subtilis=1.0：1.2：0.7：0.8；
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, 14 parts of lipase, fruit
8 parts of glue enzyme, 15 parts of cellulase.
9. the preparation method of salt-soda soil specific complex bacterial manure according to claim 6, it is characterised in that prepared by the diatomite
Method is as follows：Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 10 days, crushes 14 mesh sieves.
10. the preparation method of salt-soda soil specific complex bacterial manure according to claim 6, it is characterised in that the kitchen garbage
Preparation method is as follows：
In terms of the quality after kitchen garbage pulverizing, 3% composite bacteria agent, 0.02% mixing enzyme preparation is added, carried out aerobic solid
State is fermented, and with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, mid-term
60 DEG C of fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, collects fermentation material；
The wheat bran of fermentation material weight 5%, adjustment moisture content to 60% are added in fermentation material after addition auxin, to be carried out aerobic
Solid state fermentation, with respect to dissolved oxygen 40%, 20r/min, stage temperature control fermentation, prior fermentation temperature is 50 DEG C, and ferment 6h, in
60 DEG C of phase fermentation temperature, ferment 1h, later stage fermentation temperature 70 C, and ferment 1h, after fermentation ends, dries and crushes；
The auxin is B family vitamin；
The B family vitamin addition is the 1% of fermentation material weight；
The composite bacteria agent is made up of the microbial inoculum of following mass ratio：Bacillus licheniformis：Bacillus larvae：Natto gemma bar
Bacterium：Bacillus subtilis=1.2：1.4：0.8：1.2；
The mixing enzyme preparation, is made up of the enzyme of following parts by weight：10 parts of protease, 12 parts of amylase, 14 parts of lipase, fruit
8 parts of glue enzyme, 15 parts of cellulase；
The diatomite preparation method is as follows：
Filter residue after production pulullan polysaccharide sheet frame is degerming, normal temperature is piled up, and is dried after 20 days, crushes 14 mesh sieves.
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