CN106588255A - Gel-type fertilizer additive prepared from waste mushroom residue and preparation method thereof - Google Patents

Abstract

The invention discloses a gel-type fertilizer additive prepared from waste mushroom residues and a preparation method thereof. The gel-type fertilizer additive is prepared from, by weight, a bacillus thuringiensis preparation, a bacillus mucilaginosus inoculant, an EM inoculants, a 4 mol/L NaOH solution, potato peel, glucose, pork bone soup, urea, soya bean straw, edible mushroom residue, perlite, rapeseed cake, oyster shell, iron ore powder, rice husk, corn straw, and a proper amount of water. The fertilizer additive has adhesive property and can prevent loosening of fertilizer granules when being added to the fertilizer. The fertilizer additive also can promote soil to form colloidal granules when being added to soil. The edible mushroom residues belong to a re-fermentation process of microorganisms, so that organic substances are fully utilized and pollution due to wastes is prevented.

Description

Gel-type fertilizer additive prepared by a kind of waste bacterial slag and preparation method thereof

Technical field

The invention belongs to the Technology field of fertilizer, and in particular to the gel-type fertilizer prepared to a kind of waste bacterial slag adds Plus agent and preparation method thereof.

Background technology

The organic fertilizer of traditional sense is will to be used for the use in field, fertilizer effect generally after organic matter fermentation Fruit is to improve soil organic matter content, improves organic nutrient so as to reduce the usage amount of inorganic fertilizer, process of the fertilizer in degraded All kinds of organic acid of middle generation, can produce gel-like substance, have the effect of flocculation to soil, improve the viscous force of soil, Effect with water conservation, fertilizer conservation.The usage amount of inorganic fertilizer is reduced, and the protection and sustainable development strategies to natural environment all has Profound significance.But, not the practical effect of fertilizer is all so perfect as the aforementioned, the amount of one side fertilizer Greatly, it is that organic matter fermentation product or Organic substance are directly used in fertilizer, it is desirable to got a desired effect, and amount of application is big, is improved The input of human and material resources and time, and be easy to directly to bury less crop during administration；On the other hand, , used as the nutritional labeling after organic matter fermentation, to meeting needed for N, P, K a great number of elements of crop, crop is still for fertilizer Administration inorganic fertilizer is needed to use cooperatively.

To develop efficient, nontoxic flocculant be the important content in current field of environment engineering, and current flocculation process grinds Study carefully trend and gradually developed into the exploitation for stressing medicament now by the optimization for stressing technique in the past.Biological flocculant is a class by micro- It is biogenic to make the cohesion such as solid suspended particle not degradable in liquid, somatic cells and colloidal particle, the spy of precipitation Different macromolecule metabolite.Such flocculant is to utilize biotechnology, by having obtained from fermentable, separation and Extraction There is new, efficient, cheap, nontoxic, non-secondary pollution the water treatment agent of biological degradability and safety, can be widely applied to Feedwater and field of waste water treatment.Biological flocculant is typical environmentally friendly functional material.Microbial polysaccharide is used as flocculant Using existing some researchs, because microbial polysaccharide is safe and nontoxic, biodegradable, non-secondary pollution and species is various, and Growth of microorganism is rapid, be easily enlarged culture and realize industrialized production, so there is very big industrial applications prospect.

Colloid bacillus cereus are silicate bacterias, with the effect such as silicate mineral, Soluble phosphorus, potassium decomposing, fixed nitrogen is decomposed, are applied Have a extensive future.It can not only improve the content of titanium pigment in soil, potassium, may additionally facilitate plant growing, improve disease resistance, thus Microbial manure is its most study, using an earliest field；It goes back decomposable asymmetric choice net silicates Ore and soil mineral, Field of metallurgy can be used to extract the metal values such as Mo and Ag and carry out Ore remove impurity and flotation；In growth course it The materials such as protein, organic acid can be also generated, for producing feed supplement in feed industry；Colloid bacillus cereus extracellular polysaccharide has There is flocculation, can be used as the producing bacterial strain of biological flocculant in fields such as purified industrial waste water；

Bacillus thuringiensiss are to develop most successful microbial insecticide bacterial strain in the world at present, and Main Function target is squama wing The insects such as mesh, coleoptera, the characteristics of it possesses many other entomopathogens and does not have, such as insecticidal spectrum extensively (can prevent and treat agricultural Insect, sanitary insect pest, galleria mellonella waxmoth pest of stored grain, shade tree and ornamental plant pest, forestry pest), it is widely distributed.At present, Su Yun The production technology of golden bacillus cereuss mainly has liquid fermentation process and solid fermentation process.Solid fermentation method as reduces cost, The heuristic approach of yield is improved, conventional document there are some phases in open solid culture process and solid fermentation post processing field The research report of pass, and utilize alkali solution technique to extract insecticidal proteins, strict control alkali soluble condition is located after carrying out to solid fermentation product Reason, the method for being enriched with insecticidal proteins to improve product potency yet there are no document report.

The content of the invention

Practical function in order to improve fertilizer of the invention, the Organic substance of fermentation is extracted, then by adding it His plant growth beneficiating ingredient, makes a kind of biogel class fertilizer additive, with complexation inorganic fertilizer, extends from nature The effect of the effect time in ambient soil.

Gel-type fertilizer additive prepared by a kind of waste bacterial slag, it is characterised in that be made up of the raw material of following weight portion： Thuricade-1 4 ~ 7, colloid bacillus cereus microbial inoculum 0.5 ~ 1.0, EM bacteria agent 2 ~ 3,4mol/L NaOH solutions 10 ~ 20, Rhizoma Solani tuber osi Skin 10 ~ 15, glucose 5 ~ 10, Os Sus domestica soup 40 ~ 50, carbamide 3 ~ 6, soybean stalk 20 ~ 35, edible fungi residue 80 ~ 120, perlite 4 ~ 8, rapeseed cake 40 ~ 55, Concha Ostreae 6 ~ 10, Iron Ore Powder 3 ~ 5, rice husk 30 ~ 40, corn straw 60 ~ 80 and appropriate water.

The preparation method of gel-type fertilizer additive prepared by a kind of waste bacterial slag, it is characterised in that including following Step：

（1）Mixing soya beans straw, edible fungi residues and other following residual componentss being not directed to, high steam goes out after preliminary crushing Bacterium, is then inoculated with EM bacteria agent and thuricade-1, mixes 65 ~ 75% heap fermentation of rear water conservation thoroughly 10 ~ 15 days, grinds after air-drying Into powder；

（2）Will（1）Described powder presses solid-liquid ratio 1:3 ~ 6 dilutes, add NaOH solution to stir 30 ~ 40min, and filtering to filter Liquid, ultrasonic machine cell breakage process is carried out to filtrate；

（3）Potato peel is smashed to pieces, the water heated and boiled of mixed glucose, Os Sus domestica soup, carbamide and 3 ~ 5 times of its total weight parts, cooled down Be followed by kind of a colloid gemma bacillus agent, on 120 ~ 150r/min shaking tables under the conditions of 28 ~ 32 DEG C cultivate 60 ~ 70h, after the completion of must expand Big culture fluid, add to（2）Filtrate in, stand complexation 10 ~ 15h of free settling, after the completion of filter the solution of top 1/2 ~ 2/3, Retain lower floor's gel solution；

（4）By perlite and Concha Ostreae 1.0 ~ 1.5h of high-temperature calcination, then mix ground 150 ~ 180 mesh sieve of Iron Ore Powder, add Extremely（3）Gel solution in, stirring and evenly mixing is obtained final product into homogenate.

According to produced problem in background technology, what our company was studied focuses on：How by the product of organic matter fermentation Beneficiating ingredient is extracted, and is then refined through processing, obtains a kind of organic matter fermentation extract, and with fertilizer field is used Effect, while the characteristics of abandoning tradition amount of organic fertilizer is big, furthermore, thinking is how by organic matter fermentation extract and inorganic fertilizer With reference to, realize that the not enough problem of single fertilizer nutritional labeling is also extremely important, complement each other therebetween.

Invention mainly uses the fermentation EM bacteria agent of class, thuringiensiss and the colloid bacillus cereus, EM bacteria agent to be Composite bacteria comprising all kinds of fermented bacteriums, in order that making the more complete of organic matter fermentation, and bacillus thuringiensiss metabolism After can produce insecticidal proteins, be the strain of the best product toxic protein in the current world, invention is before this using EM bacteria agent and Soviet Union Cloud gold bacilluss mix bacterium agent carries out solid fermentation to Organic substance, then extracts tunning, and what invention was utilized is water soluble method Soak extraction, the Organic substance after solid fermentation is soluble in water, the extracellular stimulation metabolite of microbial metabolism after dissolving fermentation, There are antibiotic, toxin and be conducive to the hormoness of plant growth, while the micro- life after ultrasonic disruption machine can be utilized to fermentation Thing carries out break process, discharges cellular content, usually polysaccharide, nucleic acid, lipid and aminoacid etc., and Jing tests are repeatedly proved Add certain NaOH to allow its solubility to reach 0.2 ~ 0.8mol/L in the aqueous solution for extracting and effectively extract Su Yun gold brood cells The toxic protein that the metabolism of bacillus bacillus is produced, it is pest-resistant with good effect for fertilizer, it is eventually adding enrichment culture Colloid bacillus cereus microbial inoculum.

There are one or more layers folder steamed bun, in adding fermentation liquid, the ionic bond on its surface, hydrogen bond outside the thalline of colloid bacillus cereus And the product complexation of fermentation can be formed colloid by Van der Waals force, the sedimentation of flocculence material is formed after centrifugation, remove skim-coat More than 2/3 clear liquid, mineral nitrogen stone powder of the residual solution with ground 150 ~ 180 is mixed and is completed, colloid bacillus cereus category itself In a kind of silicate bacteria, the mineral for adding stone powder can be decomposed, with the effect such as Soluble phosphorus, potassium decomposing, fixed nitrogen, into after soil Gel is produced, the stone powder itself of addition can also play a part of fertilizer additive skeleton, maintain the stability of fertilizer.

Beneficial effects of the present invention：Fermentation process is carried out to the bacteria residue of edible fungi using compound microbial bacterial agent, then is matched somebody with somebody Close colloid bacillus cereus flocculation, make a kind of fertilizer additive with gluing characteristic, be added in fertilizer can in case Only fertiliser granulates are loose, and Zinc fractions micelle, edible fungi residue can be promoted to belong to sending out again for microorganism in itself into after soil Ferment process, sufficiently using organic materials, prevents the pollution of garbage.

Specific embodiment

Embodiment 1：

Gel-type fertilizer additive prepared by a kind of waste bacterial slag, it is characterised in that by following weight portion（Kg）Raw material make： Thuricade-1 6, colloid bacillus cereus microbial inoculum 0.7, EM bacteria agent 2,4mol/L NaOH solutions 16, potato peel 12, glucose 5 ~ 10, Os Sus domestica soup 46, carbamide 4, soybean stalk 28, edible fungi residue 100, perlite 7, rapeseed cake 45, Concha Ostreae 8, Iron Ore Powder 4th, rice husk 34, corn straw 75 and appropriate water.

The preparation method of gel-type fertilizer additive prepared by a kind of waste bacterial slag, it is characterised in that including following Step：

（1）Mixing soya beans straw, edible fungi residues and other following residual componentss being not directed to, high steam goes out after preliminary crushing Bacterium, is then inoculated with EM bacteria agent and thuricade-1, mixes 65 ~ 75% heap fermentation of rear water conservation thoroughly 10 ~ 15 days, grinds after air-drying Into powder；

（2）Will（1）Described powder presses solid-liquid ratio 1:3 ~ 6 dilutes, add NaOH solution to stir 30 ~ 40min, and filtering to filter Liquid, ultrasonic machine cell breakage process is carried out to filtrate；

（3）Potato peel is smashed to pieces, the water heated and boiled of mixed glucose, Os Sus domestica soup, carbamide and 3 ~ 5 times of its total weight parts, cooled down Be followed by kind of a colloid gemma bacillus agent, on 120 ~ 150r/min shaking tables under the conditions of 28 ~ 32 DEG C cultivate 60 ~ 70h, after the completion of must expand Big culture fluid, add to（2）Filtrate in, stand complexation 10 ~ 15h of free settling, after the completion of filter the solution of top 1/2 ~ 2/3, Retain lower floor's gel solution；

（4）By perlite and Concha Ostreae 1.0 ~ 1.5h of high-temperature calcination, then mix ground 150 ~ 180 mesh sieve of Iron Ore Powder, add Extremely（3）Gel solution in, stirring and evenly mixing is obtained final product into homogenate.

The fertilizer of soybean planting is pressed into 2:1 ratio mixes with the fertilizer additive of the present invention, by slurry-spraying pelletizing again Pelletize, for planting the test of Semen sojae atricolor, plants 5 mu of Soybean Fields, and 5 mu of Semen sojae atricolor fertilizer of matched group are left intact, and fertilising is equal According to the method that conventional soy is planted, the Semen sojae atricolor planted using the fertilizer of additive is as a result proved, the incidence rate of pest and disease damage is lower, The water conservation of soil, fertilizer-preserving ability are more preferable.

Claims (2)

1. the gel-type fertilizer additive that prepared by a kind of waste bacterial slag, it is characterised in that be made up of the raw material of following weight portion：Soviet Union Cloud gold bacillus preparation 4 ~ 7, colloid bacillus cereus microbial inoculum 0.5 ~ 1.0, EM bacteria agent 2 ~ 3,4mol/L NaOH solutions 10 ~ 20, potato peel 10 ~ 15, glucose 5 ~ 10, Os Sus domestica soup 40 ~ 50, carbamide 3 ~ 6, soybean stalk 20 ~ 35, edible fungi residue 80 ~ 120, perlite 4 ~ 8th, rapeseed cake 40 ~ 55, Concha Ostreae 6 ~ 10, Iron Ore Powder 3 ~ 5, rice husk 30 ~ 40, corn straw 60 ~ 80 and appropriate water.

2. a kind of preparation method of gel-type fertilizer additive prepared by waste bacterial slag according to claim 1, its feature exists In, including following step：

（1）Mixing soya beans straw, edible fungi residues and other following residual componentss being not directed to, high steam goes out after preliminary crushing Bacterium, is then inoculated with EM bacteria agent and thuricade-1, mixes 65 ~ 75% heap fermentation of rear water conservation thoroughly 10 ~ 15 days, grinds after air-drying Into powder；

（2）Will（1）Described powder presses solid-liquid ratio 1:3 ~ 6 dilutes, add NaOH solution to stir 30 ~ 40min, and filtering to filter Liquid, ultrasonic machine cell breakage process is carried out to filtrate；

（3）Potato peel is smashed to pieces, the water heated and boiled of mixed glucose, Os Sus domestica soup, carbamide and 3 ~ 5 times of its total weight parts, cooled down Be followed by kind of a colloid gemma bacillus agent, on 120 ~ 150r/min shaking tables under the conditions of 28 ~ 32 DEG C cultivate 60 ~ 70h, after the completion of must expand Big culture fluid, add to（2）Filtrate in, stand complexation 10 ~ 15h of free settling, after the completion of filter the solution of top 1/2 ~ 2/3, Retain lower floor's gel solution；

（4）By perlite and Concha Ostreae 1.0 ~ 1.5h of high-temperature calcination, then mix ground 150 ~ 180 mesh sieve of Iron Ore Powder, add Extremely（3）Gel solution in, stirring and evenly mixing is obtained final product into homogenate.