CN105272544A - Embedded compound bacteria fermented organic fertilizer for mulberry trees and preparation method thereof - Google Patents

Abstract

The invention discloses an embedded compound bacteria fermented organic fertilizer for mulberry trees and a preparation method thereof. The organic fertilizer of the invention is prepared from the following raw materials: cellulose, a compound microbial agent, plant ash, palm oil, rice hull, rapeseed cake, dried cow dung, clay, urea, potassium sulfate, a microbial embedding medium and a proper amount of water. The embedded compound bacteria fermented organic fertilizer is specifically an organic fertilizer prepared by semi-fermentation. A special material is used to embed cellulose and the microbial agent, and then the embedded finished product is used to ferment organic matter so as to finally prepare the organic fertilizer.

Description

Organic Mulberry fertilizer of a kind of embedding compound bacteria-fermented and preparation method thereof

Technical field

The invention belongs to the Technology field of fertilizer, be specifically related to organic Mulberry fertilizer of a kind of embedding compound bacteria-fermented and preparation method thereof.

Background technology

Microbial fertilizer can Some substitute chemical fertilizer, reduce it and use the loss and waste that cause in a large number, even cause salting of soil and contaminate environment, the formulation that microbial fertilizer is common or the bacterium liquid for receiving after fermentation, or be the absorption microbial inoculum that carrier is made with the peat composed of rotten mosses, two kinds of formulations are more convenient in preparation, but all there is many deficiencies, after microbial inoculum is manured into soil, under thalline is directly exposed to open state, easily cause thalline dead, to certain level, not easily reach if make viable bacteria set propagation at short notice.Entrapping method is one of immobilized cell technique grown up in recent years, be microorganism is embedded in be rich in nutrition, to outside environmental facies to close particle in, concerning inner microorganism, particle defines a microenvironment, shock absorption is risen to outside atmosphere, the microenvironment of particle is conducive to the propagation of microorganism, is easy to form dominant microflora, is conducive to the survival of artificial inoculation microorganism and the ecologic competition with soil bacteria.

Summary of the invention

In order to solve in the past, embedded particles colloid is too soft, cannot the feature that uses of mixed fertilizer, and the present invention studies new technology and imbedded microbe is used for fertiliser production, improves the activity of microorganism in soil and properties, realizes particular by following methods:

The organic Mulberry fertilizer of a kind of embedding compound bacteria-fermented, it is characterized in that, be made up of the raw material of following weight part: cellulase 2 ~ 5, complex micro organism fungicide 8 ~ 10, plant ash 30 ~ 35, plam oil 8 ~ 10, rice husk 60 ~ 80, rapeseed cake 80 ~ 120, dry cow dung 45 ~ 60, clay 30 ~ 50, urea 15 ~ 20, potassium sulfate 25 ~ 40, microorganism embedding medium 12 ~ 20 and appropriate water;

Described microorganism embedding medium is made up of with 3: 1 ratios jointly a liquid and b liquid, and a liquid is that mixing sodium alginate 6 ~ 10 and polyvinyl alcohol 8 ~ 12 add in the water of 40 ~ 50 times of its weight parts, heats 70 ~ 80 DEG C of stirring and dissolving and makes, be incubated 28 DEG C for subsequent use; B liquid is Calcium Chloride Powder Anhydrous 5 ~ 6, the peat composed of rotten mosses 3 ~ 7, limestone powder 12 ~ 16, linseed oil 8 ~ 12, acrylate 15 ~ 20, ethanol 14 ~ 18 and appropriate water; Preparation method is in a kettle. by ethanol thin up 6 ~ 8 times, then acrylate is added, be heated to 80 ~ 100 DEG C of stirring and dissolving, then Calcium Chloride Powder Anhydrous, the peat composed of rotten mosses, limestone powder and linseed oil is blended into, and effect 0.5 ~ 2h carries out composite under 300 ~ 500r/min rotating speed heat-retaining condition, become uniform emulsion, be incubated 28 DEG C for subsequent use.

Embed a preparation method for the organic Mulberry fertilizer of compound bacteria-fermented, it is characterized in that, comprise following step:

(1) mix plant ash, rice husk, rapeseed cake, dry cow dung and clay, spray a certain amount of water-wet, overall moisture content 55 ~ 60%, is squeezed into scoriform;

(2) complex micro organism fungicide and cellulase are added in the microorganism embedding medium a liquid under keeping warm mode, vibrate mixing 10 ~ 15min on thermostatic mixer, and mixing, then adds microorganism embedding medium b liquid, continue vibration 20 ~ 30min, then leave standstill 30 ~ 40min and embed;

(3) entirety unification is carried out 800 ~ 1000r/min after (2) complete centrifugal, lower sediment is led to hot-air seasoning, final powder is broken into 20 ~ 40 mesh sieve size particles, after particle and plam oil are soaked, add in the slag charge described in (1), mixing, under 38 ~ 45 DEG C of temperature condition, heap fermentation becomes half fermentation material in 5 ~ 10 days;

(4) add in the fermentation material described in (3) after urea, potassium sulfate and other remaining component being mixed, mixing is dispersed evenly, last granulation.

According to the further investigation to microorganism embedding techniques, find sodium alginate (SA) and polyvinyl alcohol (PVA) is water-soluble in a heated condition and viscosity is lower, in the solution can with Ca ²⁺, Ba ²⁺, Fe ³⁺plasma effect is cross-linked into internal voids spheroidal particle of uniform size, wherein Ca by forming multiple coordinate bond ²⁺effect best and with low cost;

Research finds, when embedding bacterial classification with above embedding medium, suitably to add some auxiliary materials and enter SiO ₂or CaCO ₃deng, utilize auxiliary material to be insoluble in the characteristic of water, the partial mesh topology of embedding can be destroyed, make inner hole become large, increase particle permeability and mass transfer performances, improve cell release rate, the physical strength of particle can be improved simultaneously.

The present invention proves feasibility in SA and PVA practical application and unusual effect by experiment, concrete grammar is as follows: at 35 DEG C, 9 concentration proportionings are combined into respectively one by one with 1.0%, 2.0%, 3.0% of 2.5% of SA, 3.0%, 3.5% and PVA, configuration 10ml solution, take wet thallus 1.0g, add the embedding medium solution of above different concns proportioning, in constant temperature blender with magnetic force, vibration mixing, is evenly added drop-wise to 20ml with the main emperorship of 8# syringe needle according to certain speed and contains 3.0CaCl ₂saturated boric acid solution in, crosslinked 24h, leaches particle, priority physiological saline and sterilized water wash 10 times, take 1.0g particle in YMY nutrient solution, in 28 DEG C, 180r/min, multiplication culture 72h, measure viable bacteria in particle ripe, remainder particulate is placed in sterilized water, and 4 DEG C of preservations are for subsequent use, each process 3 times, results averaged.

Take fresh embedded particles and each 1.0g of particle through multiplication culture, respectively in the citric acid solution of 0.2mol/L, be settled to 10ml, fully vibrate to particle with vartex vibrator and dissolve completely, make the whole stripping of thalline, cultivate 3d with dilution-plate method in 28 DEG C, enumeration, each process repeats for 3 times, results averaged, and indices calculates as follows:

Particle viable count (cfu/g)=(certain dilution viable count × extension rate × bacterium liquid number)/particle weight

Particle viable count before particle viable count/propagation after proliferation times=increment

Embedding rate (%)=the be embedded in viable count in particle/total viable count × 100

Invention is reached a conclusion: embedded particles reaches 4.8 × 10 through multiplication culture living bacteria count ¹¹more than cfu/g, embed bacterial classification gained embedded particles with SA and improve 3 times, use SA with PVA that active sludge is embedded into particle respectively, the particle mass transfer velocity time reached needed for maximum value of SA and PVA ball is 25min and 45min;

In over-all properties, larger improvement can be had compared with the embedding of single-material with the use of SA and PVA simultaneously, more aspect is all outstanding for mechanical property, viable bacteria rate of mass transfer and activity etc. simultaneously, improved based on this, make the embedding medium that biological bacteria is special, be made up of embedding composition a liquid part and moulding section b liquid, a liquid is experimentally a certain amount of SA and PVA of result proportioning, and realize effect best, b liquid is Ca ²⁺, limestone powder, resin compositing monomer, vegetables oil and some solvent compositions, Ca ²⁺effect is cross-linked into internal voids spheroidal particle of uniform size by forming multiple coordinate bond, has the crosslinked effect improving toughness, and the CaCO in limestone powder ₃particle can make the hole embedding whole interior become large, increase particle permeability and mass transfer performances, improve cell release rate, the physical strength of particle can be improved simultaneously, resin monomer add the deficiency that can make up SA and PVA plastic character, strengthen state of cure, be easy to the suitability for industrialized production of fertilizer, vegetables oil is conducive to the emulsification of b liquid, is not easily cross-linked before using.

Beneficial effect of the present invention: the present invention is a kind of organic fertilizer made that partly ferments specifically; after utilizing special substance to embed cellulase and microbiobacterial agent; the finished product fermentation of recycling embedding is organic; finally make; it is active that bacterial classification after embedding and enzyme have stronger vitality; be not subject to the impact of environmental factors; add in organic matter; first ferment for some time; adapt to and adsorb new environment; then use residue organic at soil relaying supervention ferment, improve soil organic content, protection of the environment.

Embodiment

Embodiment 1

The organic Mulberry fertilizer of a kind of embedding compound bacteria-fermented, it is characterized in that, be made up of the raw material of following weight part (Kg): cellulase 4, complex micro organism fungicide 8, plant ash 35, plam oil 8, rice husk 80, rapeseed cake 80, dry cow dung 45, clay 30, urea 20, potassium sulfate 30, microorganism embedding medium 15 and appropriate water;

Described microorganism embedding medium is made up of with 3: 1 ratios jointly a liquid and b liquid, and a liquid is that mixing sodium alginate 8 and polyvinyl alcohol 10 add in the water of 40 ~ 50 times of its weight parts, heats 70 ~ 80 DEG C of stirring and dissolving and makes, be incubated 28 DEG C for subsequent use; B liquid is Calcium Chloride Powder Anhydrous 6, the peat composed of rotten mosses 5, limestone powder 15, linseed oil 10, acrylate 16, ethanol 16 and appropriate water; Preparation method is in a kettle. by ethanol thin up 6 ~ 8 times, then acrylate is added, be heated to 80 ~ 100 DEG C of stirring and dissolving, then Calcium Chloride Powder Anhydrous, the peat composed of rotten mosses, limestone powder and linseed oil is blended into, and effect 0.5 ~ 2h carries out composite under 300 ~ 500r/min rotating speed heat-retaining condition, become uniform emulsion, be incubated 28 DEG C for subsequent use.

Embed a preparation method for the organic Mulberry fertilizer of compound bacteria-fermented, it is characterized in that, comprise following step:

(1) mix plant ash, rice husk, rapeseed cake, dry cow dung and clay, spray a certain amount of water-wet, overall moisture content 55 ~ 60%, is squeezed into scoriform;

(2) complex micro organism fungicide and cellulase are added in the microorganism embedding medium a liquid under keeping warm mode, vibrate mixing 10 ~ 15min on thermostatic mixer, and mixing, then adds microorganism embedding medium b liquid, continue vibration 20 ~ 30min, then leave standstill 30 ~ 40min and embed;

(3) entirety unification is carried out 800 ~ 1000r/min after (2) complete centrifugal, lower sediment is led to hot-air seasoning, final powder is broken into 20 ~ 40 mesh sieve size particles, after particle and plam oil are soaked, add in the slag charge described in (1), mixing, under 38 ~ 45 DEG C of temperature condition, heap fermentation becomes half fermentation material in 5 ~ 10 days;

(4) add in the fermentation material described in (3) after urea, potassium sulfate and other remaining component being mixed, mixing is dispersed evenly, last granulation.

Use the organic Mulberry fertilizer of embedding compound bacteria-fermented of the present invention, for planting 5 mu of mulberry tree fields, as experimental group, choose all identical mulberry tree field, other 5 mu of several aspects of growing way for contrast simultaneously, result shows, the mulberry tree growing way of experimental group and everyway more effective than control group, the mulberry leaf disease and pest that produces of time reduces and blade is fresh and tender, reduces Fertilizer application amount and labor management cost simultaneously.

Claims (2)

1. the organic Mulberry fertilizer of embedding compound bacteria-fermented, it is characterized in that, be made up of the raw material of following weight part: cellulase 2 ~ 5, complex micro organism fungicide 8 ~ 10, plant ash 30 ~ 35, plam oil 8 ~ 10, rice husk 60 ~ 80, rapeseed cake 80 ~ 120, dry cow dung 45 ~ 60, clay 30 ~ 50, urea 15 ~ 20, potassium sulfate 25 ~ 40, microorganism embedding medium 12 ~ 20 and appropriate water;

Described microorganism embedding medium is made up of with 3: 1 ratios jointly a liquid and b liquid, and a liquid is that mixing sodium alginate 6 ~ 10 and polyvinyl alcohol 8 ~ 12 add in the water of 40 ~ 50 times of its weight parts, heats 70 ~ 80 DEG C of stirring and dissolving and makes, be incubated 28 DEG C for subsequent use; B liquid is Calcium Chloride Powder Anhydrous 5 ~ 6, the peat composed of rotten mosses 3 ~ 7, limestone powder 12 ~ 16, linseed oil 8 ~ 12, acrylate 15 ~ 20, ethanol 14 ~ 18 and appropriate water; Preparation method is in a kettle. by ethanol thin up 6 ~ 8 times, then acrylate is added, be heated to 80 ~ 100 DEG C of stirring and dissolving, then Calcium Chloride Powder Anhydrous, the peat composed of rotten mosses, limestone powder and linseed oil is blended into, and effect 0.5 ~ 2h carries out composite under 300 ~ 500r/min rotating speed heat-retaining condition, become uniform emulsion, be incubated 28 DEG C for subsequent use.

2. a kind of preparation method embedding the organic Mulberry fertilizer of compound bacteria-fermented according to claim 1, is characterized in that, comprise following step:

(1) mix plant ash, rice husk, rapeseed cake, dry cow dung and clay, spray a certain amount of water-wet, overall moisture content 55 ~ 60%, is squeezed into scoriform;

(2) complex micro organism fungicide and cellulase are added in the microorganism embedding medium a liquid under keeping warm mode, vibrate mixing 10 ~ 15min on thermostatic mixer, and mixing, then adds microorganism embedding medium b liquid, continue vibration 20 ~ 30min, then leave standstill 30 ~ 40min and embed;

(3) entirety unification is carried out 800 ~ 1000r/min after (2) complete centrifugal, lower sediment is led to hot-air seasoning, final powder is broken into 20 ~ 40 mesh sieve size particles, after particle and plam oil are soaked, add in the slag charge described in (1), mixing, under 38 ~ 45 DEG C of temperature condition, heap fermentation becomes half fermentation material in 5 ~ 10 days;

(4) add in the fermentation material described in (3) after urea, potassium sulfate and other remaining component being mixed, mixing is dispersed evenly, last granulation.