CN104496707A - Liquid composite biological multi-control fertilizer, and preparation method and application thereof - Google Patents
Liquid composite biological multi-control fertilizer, and preparation method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.
Description
Technical field
The present invention relates to technical field of biological fertilizer, particularly a kind of for paddy rice, corn, wheat and fruits and vegetables seed soaking, foliage-spray or the multi-control fertilizer of liquid compound bio of filling with root and preparation method thereof and application.
Background technology
Along with a large amount of uses of chemical fertilizer particularly nitrogenous fertilizer, be enriched more nitrate in grain, nutritional quality and mouthfeel decline all to some extent, can not meet the health diet consumers demand that people pursue nutritious, tasty, nuisanceless meals.Long-term a large amount of use chemical fertilizer can make soil inorganic salts contg increase, organic matter reduces, and soil loses acid base equilibrium, and soil property hardens; And humic acid, moisture and the nutrient famine in soil, cause soil desertification; Fertilizer is crop " grain ", " nutrition ", is directly connected to the output of crop and the quality of product.Now both at home and abroad all in develop actively green agriculture, the ecological agriculture, advocate production safety, pollution-free green food.In the process of producing green food, then requirement or need not use chemical fertilizer, chemical pesticide and other chemical substance as far as possible less.
For solving the problem, China has dropped into a large amount of manpower and materials and has studied.Composite microbic bacterial fertilizer, especially biological multi-control fertilizer is more and more subject to the favor of people as environmental friend fertilizer, it not only directly supplies a large number of nutrients that crop needs, but also it is actively movable by a large amount of microorganism lived, the nutritive substance providing crop to need or generation hormone, to stimulate plant growth, have biological pesticide, bio-feritlizer and biological nutrition three efficacy simultaneously.
At present, the research of concerns about bio compound manure or biological multi-control fertilizer has: patent documentation CN1970506A, multielement compound microbe bacterial fertilizer; CN102826895A, a kind of multielement compound microbe bacterial fertilizer and preparation method thereof; CN103204732A, containing composite microbic bacterial fertilizer and preparation method thereof and the application of polyglutamic acid Producing Strain; CN102910985A, a kind of Organic compound microbial fertilizer and preparation method thereof, microbial-bacterial fertilizer is made up of zymocyte liquid, turfy soil, Chicken dung and Secondary ammonium phosphate; CN102584453A, a kind of composite microbic bacterial fertilizer and preparation method thereof, is conciliate inorganic phosphorus bacterium formed by plant ash, fermention medium and self vinelandii formed, solution organophosphorus bacterium; CN102951966A, a kind of production method of composite microbic bacterial fertilizer, it be adopt through the microbiobacterial agent of vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria and the quail excrement of fermentation and vinasse be prepared by main raw material; CN102910988A, a kind of complex microorganism liquid bacterial fertilizer preventing and treating plant pest and preparation method thereof, the preparations such as Chinese prickly ash, capsicum, Ginkgo Leaf, kuh-seng, garlic of being fermented by symbiotic co-existence beneficial flora; CN101851123A, a kind of high-concentration compound microorganism bacterial fertilizer and production method thereof, the microbial inoculum of blown-ball Azotobacter, bacillusmusilaginosiengineering, bacillus megaterium, Bacillus licheniformis, Bacillus macerans is cultivated respectively and fermentation standby.
The composite microbiological fertilizer that market is applied is less, but effect is fine.Such as, the composite microbiological fertilizer that Bao Nong bio tech ltd, Hong Kong produces, using ocean high reactivity fish-protein and marine alga as main organic matter material, adds multiple biocontrol microorganisms and function yeast, be added with amino acid and the necessary Chelating state Meso-and micro-nutrients of plant growth simultaneously, there is enhancing soil activation, suppress and prevent the generation of soil-borne disease, strengthen crop resistance, fixing nitrogen, dissolving phosphor and dissolving potassium, improve comprehensive plant recovery of nutrient, oil recovery enhancement, improve effect of output.The Enteromorpha bio-bacterial manure also having Qingdao Seawin Biotech Group Co., Ltd. to produce, containing 5 strain probioticss, can generate dominant microflora after entering soil fast, forms the microecological balance system of soil health, strengthens the disease resistance of trees; Be rich in the multiple nutritive substance useful to arboreal growth simultaneously, as organic matter, sea grass polysaccharide, enteromorpha oligosaccharide, enteromorpha fiber and multiple medium trace element, for trees provide balanced nutritious, strengthen the immunizing power of trees body, prevent later stage various soil-borne disease, flourishing.For another example the NEW TYPE OF COMPOSITE bacterial classification that is combined with Taiwan promise Ka Shi actinomycetes of Japanese silicate bacteria, fungus treatment forms three-dimensional protective barrier around crop root, the growth and breeding of rapid suppression soil surface characters, cover crop root system, to the dead seedling caused because of continuous cropping and soil-borne disease, dead, rotten etc. remarkably productive, purification soil effectively can decompose crop residues and residue decomposes the soil poisons such as the organic acid produced, thus the fast quick-recovery of crop is grown up strong and sturdy, continuous use continuous cropping hymexazol, soil compaction can be eliminated, chessom, reduce soil alkaline to poison, biological inoculum is to the N solidified in soil simultaneously, P, K and the release of various trace elements rapid solution, balanced soil nutrient, the stiff seedling of Rapid reversal, weak seedling, seedling, the various nutritional deficiency phenomenon such as Huang Ye.
However, can play the multi-control fertilizer of biology of effective combination of microbial-bacterial fertilizer and organic fertilizer, becoming the best collocation of the problem now that solves undoubtedly, is also the developing direction of following Term Fertilization.Therefore, exploitation efficient, low toxicity, low residue, low cost, the multi-control fertilizer of environment amenable biology have become the development trend of modern fertilizer.
Summary of the invention
The object of this invention is to provide the multi-control fertilizer of a kind of liquid compound bio.
Another object of the present invention is to provide the purposes of the multi-control fertilizer of liquid compound bio.
Another object of the present invention is to provide the preparation method of the multi-control fertilizer of liquid compound bio.
The present invention use microbial inoculum be China General Microbiological culture presevation administrative center version in 1997 bacterial classification catalogue disclosed in bacterial classification, numbering and bacterial strain name be:
Bacterium numbering is the trichoderma viride (Trichoderma viride Persoon exFries) of CGMCC3.2942, bacterium numbering is the streptomyces hygroscopicus (Streptomyces hygroscopicusWaksman et Henrici) of CGMCC4.1114, bacterium numbering is the brown streptomycete of Chinese cassia tree (Streptomycescinereohygroscopicus Yan et al.) of CGMCC4.1099, bacterium numbering is the bacillus amyloliquefaciens (Bacillus amyloliquefaciens Priest et al.) of CGMCC1.1226, bacterium numbering is the Bacillus sphaericus (Bacillus sphaericus Meyer et Neide) of CGMCC1.929, bacterium numbering is bacillusmusilaginosiengineering (the Bacillus mucilaginosus Krasil of CGMCC1.910, nikov), bacterium numbering is the blown-ball Azotobacter (Azotobacter chroococcum Beijerinck) of CGMCC1.212, bacterium numbering is the golden streptomyces chromogenes (Streptomyces aureochromogenes Yan et al.) of CGMCC4.910.
The object of the invention is to be realized by following technical proposals:
According to the multi-control fertilizer of liquid compound bio of the present invention, it is characterized in that, it is made up of the feed composition of following weight:
Streptomyces hygroscopicus liquid 1 ~ 3 part, Chinese cassia tree brown streptomycete liquid 1 ~ 3 part, bacillus amyloliquefaciens liquid 2 ~ 4 parts, Bacillus sphaericus liquid 2 ~ 4 parts, bacillusmusilaginosiengineering liquid 7 ~ 15 parts, blown-ball Azotobacter liquid 1 ~ 3 part, golden streptomyces chromogenes liquid 1 ~ 3 part, the fermentative degradation liquid 8 ~ 10 parts of trichoderma viride, matrix 8 ~ 10 parts and sterilized water 45 ~ 65 parts
Wherein, the fermentation process of the fermentative degradation liquid of trichoderma viride is: by weight ratio, takes 15 parts of solid mediums, load in fermentation vat, add 9 parts of tap water, make water content reach 60%, pH value 7.0, stirs, mixes, at 121 DEG C, sterilizing 20 minutes, is down to room temperature, then 3.6 parts of viride bacterial classifications are inoculated in solid medium top fermentation, culture temperature 25 ~ 27 DEG C, incubation time 56 ~ 72 hours, the degradation product fermented sterilizing 20 minutes after filtration and at 121 DEG C, to obtain final product;
Solid medium is discarded after rod is pulverized by edible fruits waste residue, straw or edible mushrooms and is prepared, edible fruits waste residue refers to the fruit waste residue of Nanguo Pear, white pear or apple, straw refers to the stalk of paddy rice, wheat, Chinese sorghum or corn, and edible mushrooms discards the discarded rod of edible mushrooms that rod refers to mushroom, Pleurotus eryngii or Coprinus comatus;
Matrix is water-soluble humic acid or the one of becoming thoroughly decomposed in feces of livestock and poultry water or two kinds of mixtures;
The feces of livestock and poultry water that becomes thoroughly decomposed refers to the ight soil water that becomes thoroughly decomposed of ox, horse or sheep class animal and chicken, duck or bird class;
Sterilized water refers to that seawater and freshwater is that 1:180 ~ 220 mix according to weight proportion, the sterilizing water of 20 minutes at 121 DEG C.
According to the multi-control fertilizer of liquid compound bio of the present invention, it is characterized in that, it is made up of the feed composition of following weight:
Streptomyces hygroscopicus liquid 2 ~ 3 parts, Chinese cassia tree brown streptomycete liquid 2 ~ 3 parts, bacillus amyloliquefaciens liquid 2 ~ 4 parts, Bacillus sphaericus liquid 2 ~ 4 parts, bacillusmusilaginosiengineering liquid 9 ~ 13 parts, blown-ball Azotobacter liquid 2 ~ 3 parts, golden streptomyces chromogenes liquid 2 ~ 3 parts, the fermentative degradation liquid 9 ~ 10 parts of trichoderma viride, matrix 9 ~ 10 parts and sterilized water 47 ~ 61 parts
Wherein, the fermentation process of the degradation solution of trichoderma viride is same as above;
Matrix be water-soluble humic acid and Chicken dung just water weight proportion be the mixture of 1:1 ~ 1.2;
Sterilized water refers to that seawater and freshwater is that 1:190 ~ 210 mix according to weight proportion, the sterilizing water of 20 minutes at 121 DEG C.
According to the purposes of the multi-control fertilizer of liquid compound bio of the present invention, be applied to paddy rice, corn, wheat and fruits and vegetables seed soaking, foliage-spray or fill with root.
According to the preparation method of the multi-control fertilizer of liquid compound bio of the present invention, it is characterized in that, its preparation process is as follows:
The preparation of A, trichoderma viride fermentative degradation liquid
By weight ratio, take 15 parts of solid mediums, load in fermentation vat, add 9 parts of water, water content is made to reach 60%, pH value 7.0, stir, mix, sterilizing 20 minutes at 121 DEG C, be down to room temperature, again 3.6 parts of viride bacterial classifications are inoculated in solid medium top fermentation, culture temperature 25 ~ 27 DEG C, incubation time 56 ~ 72 hours, for keeping moisture in fermenting process, and being uniformly distributed of moisture, adopt within every two days, in solid medium, spray into a small amount of aqua sterilisa, water content is made to keep 60%, and stirred, to make fermentation complete, in order to ensure the ventilation in fermentation, four layers of Breathable nonwoven are adopted to cover Chi Kou, the degradation product fermented sterilizing 20 minutes after filtration and at 121 DEG C, obtain,
B, various feed composition are prepared burden by its weight:
Obtained trichoderma viride fermentative degradation liquid is mixed with described matrix and sterilized water, add streptomyces hygroscopicus liquid, Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid and golden streptomyces chromogenes liquid again, mix, obtain the multi-control fertilizer of liquid compound bio;
Wherein the weight of feed composition is same as above.
The present invention above-mentioned streptomyces hygroscopicus liquid, Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid and golden streptomyces chromogenes liquid adopt conventional method preparation.
1, such as, a kind of fermentation process preparing above-mentioned blown-ball Azotobacter liquid:
--second order fermentation tank spreads cultivation tapered shaking flask enlarged culturing--one grade fermemtation tank (seeding tank) liquid spreads cultivation--preparation technology: slant strains activation culture.(each step needs 20 ~ 48 hours).
Substratum: beef extract-peptone liquid nutrient medium, culture temperature 30 DEG C, rotating speed are 60 ~ 120 turns/min, pH be 7.2 ~ 7.8, incubation time 20 ~ 48 hours, through inspection, after the quantity of each effective bacterium must not be less than 0.01 hundred million/mL, reach more than 2.0 hundred million/milliliter (standard GB/T-20287-2006) containing total living bacteria count, can be used as production bacterium liquid for subsequent use.
Preparation Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid and bacillusmusilaginosiengineering liquid fermentation process are with the fermentation process of above-mentioned blown-ball Azotobacter liquid.
A kind of fermentation process preparing above-mentioned streptomyces hygroscopicus liquid:
--second order fermentation tank spreads cultivation tapered shaking flask enlarged culturing--one grade fermemtation tank (seeding tank) liquid spreads cultivation--preparation technology: slant strains activation culture.(each step needs 120 ~ 144 hours).
Substratum: Gause I liquid nutrient medium, culture temperature 28 DEG C, rotating speed are 60 ~ 120 turns/min, pH be 7.0 ~ 7.2, incubation time 120 ~ 240 hours, through inspection, after the quantity of each effective bacterium must not be less than 0.01 hundred million/mL, reach more than 2.0 hundred million/milliliter (standard GB/T-20287-2006) containing total living bacteria count, can be used as production bacterium liquid for subsequent use.
Prepare the fermentation process of golden streptomyces chromogenes liquid fermentation process with above-mentioned streptomyces hygroscopicus liquid.
The present invention be utilize bacterium numbering be 3.2942 trichoderma viride, through fermentative degradation, preparation is easy to carbohydrate that farm crop absorb and organic trichoderma viride fermentative degradation liquid.
The invention provides the microorganism species of efficient useful phosphorus decomposing, potassium decomposing, fixed nitrogen, disease resisting effect, biological pesticide, bio-feritlizer and the effect of biological nutrition three efficacy can be played simultaneously, toxicological harmless again, free from environmental pollution.
Test of the present invention proves that the microorganism strains used has following effect:
Streptomyces hygroscopicus can produce antifungal antibiotic, suppresses Rhizoctonia solani Kuhn, Pyricularia oryzae and fusarium graminearum.
The brown streptomycete of Chinese cassia tree is a kind of bacterial manure, can transform n-p-k element in soil, increase soil fertility, and has stimulation plant growth effect.
Bacillus amyloliquefaciens can improve crop to the Scavenging activity producing oxygen in germ and adverse circumstance harm primosome, regulates cell microorganism environment, maintains the normal physiological metabolism of cell and biochemical reaction, improve the resistance of crop, increase the health-care effect of crop, can plant growth be promoted, improve output.
Bacillus sphaericus is that a kind of energy produces bacterium insect to single-minded insecticidal activity albumen, especially prevents and treats soil nematodes.
Bacillusmusilaginosiengineering is a kind of B. mucilaginocus, and its effect is by the mineral K in soil, is dissolved into the potassium that directly can be absorbed by plant.
Blown-ball Azotobacter is a kind of bacterial fertilizer, can secrete growth hormone, and the nitrogen in air can be converted into the absorbent ammonium nitrogen of farm crop, nitric nitrogen and organonitrogen by process of production, thus creates fertilizer efficiency.
Gold streptomyces chromogenes can produce antifungal antibiotic, suppresses Rhizoctonia solani Kuhn and Pyricularia oryzae.
Described various bacterium liquid is pressed suitable proportion mixture by the present invention, and each Microflora ratio in composite fungus agent can be kept well relatively stable.Composite fungus agent is with the microorganism species relying on physical environment to carry out decomposing and compared with the flora of the mixture without single culture, to adopt after single culture mixture flora together again, due to rational matching ratio can be selected according to actual needs, biological nature and the mutual synergy of each flora self in microbial inoculum can be played fully, so carry out the scope of application of carrying out a biological disposal upon widely with it.
The multi-control fertilizer of liquid compound bio prepared by the present invention, reaches more than 2.0 hundred million/milliliter (standard GB/T-20287-2006) containing total living bacteria count.
The present invention utilizes trichoderma viride, adopt solid-state aerobic fermentation, degraded edible fruits waste residue, straw or edible mushrooms discard polysaccharide in rod, carbohydrate and Mierocrystalline cellulose, introduce efficient useful phosphorus decomposing, potassium decomposing, fixed nitrogen, anti-disease microbial flora, and, by the control to fermentation condition, the nutriment in a fertilizer of biological multi-control fertilizer is made to reach highest level.Play biological pesticide, biological fertilizer efficiency and the effect of biological nutrition three efficacy simultaneously, and, toxicological harmless, free from environmental pollution.
Especially, the present invention utilizes Nanguo Pear waste residue, rice straw or Lentinus Edodes fungus discard excellent fermentative degradation, with fixed nitrogen, phosphorus decomposing, the active complex microorganism of potassium decomposing, and to rice blast, rice bakanae disease and rice sheath blight disease, wheat scab, root rotof flax, the leaf blight of corn, corn southern leaf blight has compound actinomycete agent and the mixing of composite bacterial microbial inoculum of antagonistic action, not only for improving soil microorganisms group structure, activating soil nutrient, improve From The Rhizosphere of Rice nutrition, improve yield and quality of rice, improve water quality, more can promote the growth of aquatic in rice terrace.
The multi-control fertilizer of liquid compound bio of the present invention, is used for seed soaking, foliage-spray or fills with root simultaneous test, obtaining positive effect as follows through field:
(1) containing abundant organic and humic acid, can Soil structure be improved, vegetable soil, promote that soil is built up fertility.
(2) with other fertilizer ratio such as nutrient such as grade, crop yield 20%.
(3) formulate and coordinate farm crop absorption nutrition, stimulating and regulation and control plant growth, reduce or reduce crop pest, affecting crop quality.
(4) crab making to cultivate in rice terrace, loach, swamp eel net gain 10%.
(5) or fertilizer amount need not be reduced, be beneficial to protection of the environment.
Embodiment
Following embodiment, only for explaining the present invention, does not form the restriction to right, other alternative means that those skilled in the art can expect according to description, all should within the protection domain of the claims in the present invention.
The microbial strains that the embodiment of the present invention utilizes, for the trichoderma viride of fermentative degradation, and phosphorus decomposing, potassium decomposing, fixed nitrogen, disease resisting effect microorganism species, bacterial classification disclosed in the bacterial classification catalogue selecting China General Microbiological culture presevation administrative center version in 1997.
Its numbering and bacterial strain name are: bacterium numbering is the trichoderma viride (Trichodermaviride Persoon ex Fries) of CGMCC3.2942, bacterium numbering is the streptomyces hygroscopicus (Streptomyceshygroscopicus Waksman et Henrici) of CGMCC4.1114, bacterium numbering is the brown streptomycete of Chinese cassia tree (Streptomyces cinereohygroscopicus Yan et al.) of CGMCC4.1099, bacterium numbering is the bacillus amyloliquefaciens (Bacillus amyloliquefaciens Priest et al.) of CGMCC1.1226, bacterium numbering is the Bacillus sphaericus (Bacillus sphaericus Meyer et Neide) of CGMCC1.929, bacterium numbering is bacillusmusilaginosiengineering (the Bacillus mucilaginosus Krasil of CGMCC1.910, nikov), bacterium numbering is the blown-ball Azotobacter (Azotobacter chroococcum Beijerinck) of CGMCC1.212, bacterium numbering is the golden streptomyces chromogenes (Streptomyces aureochromogenes Yan etal.) of CGMCC4.910.
The preparation method of the multi-control fertilizer of liquid compound bio of the embodiment of the present invention 1 ~ 9 is following steps:
The preparation of A, trichoderma viride fermentative degradation liquid
By weight ratio, take 15 parts of solid mediums, load in fermentation vat, add 9 parts of water, water content is made to reach 60%, pH value 7.0, stir, mix, sterilizing 20 minutes at 121 DEG C, be down to room temperature, again 3.6 parts of viride bacterial classifications are inoculated in solid medium top fermentation, culture temperature 25 DEG C, incubation time 60 hours, for keeping moisture in fermenting process, and being uniformly distributed of moisture, adopt within every two days, in solid medium, spray into a small amount of aqua sterilisa, water content is made to keep 60%, and stirred, to make fermentation complete, in order to ensure the ventilation in fermentation, four layers of Breathable nonwoven are adopted to cover Chi Kou, the degradation product fermented sterilizing 20 minutes after filtration and at 121 DEG C, obtain,
B, various feed composition are prepared burden according to the weight described in embodiment:
By trichoderma viride fermentative degradation liquid obtained above and described matrix and sterilized water, mix, add streptomyces hygroscopicus liquid, Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid again, mix, obtain the multi-control fertilizer of liquid compound bio.
In preparation method, solid medium is discarded after rod is pulverized by edible fruits waste residue, straw or edible mushrooms and is prepared, and edible fruits waste residue refers to the waste residue of Nanguo Pear, and straw refers to the stalk of paddy rice, and edible mushrooms discards the discarded rod that rod refers to Lentinus Edodes fungus;
Matrix refer to water-soluble humic acid and Chicken dung just water be the mixture of 1:1 according to weight proportion;
Sterilized water refers to that seawater and freshwater is 1:200 mixing according to weight proportion, the sterilizing water of 20 minutes at 121 DEG C.
The preparation method of the respective fermented liquid of the above-mentioned streptomyces hygroscopicus liquid of the embodiment of the present invention 1 ~ 9, Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid is as follows:
The fermentation process preparing above-mentioned blown-ball Azotobacter liquid is: and beef extract-peptone liquid culture medium, culture temperature 30 DEG C, rotating speed are 60 turns/and min, pH be 7.5, incubation time 25 hours, through inspection, through inspection, after the quantity of each effective bacterium must not be less than 0.01 hundred million/mL, reach more than 2.0 hundred million/milliliter (standard GB/T-20287-2006) containing total living bacteria count, can be used as production bacterium liquid for subsequent use.
Preparation Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid and bacillusmusilaginosiengineering liquid fermentation process are with the fermentation process of above-mentioned blown-ball Azotobacter liquid.
The fermentation process preparing above-mentioned streptomyces hygroscopicus liquid is: and Gause I liquid culture medium, culture temperature 28 DEG C, rotating speed are 60 turns/and min, pH be 7.0, incubation time 240 hours, through inspection, after the quantity of each effective bacterium must not be less than 0.01 hundred million/mL, reach more than 2.0 hundred million/milliliter (standard GB/T-20287-2006) containing total living bacteria count, can be used as production bacterium liquid for subsequent use.
Prepare the fermentation process of golden streptomyces chromogenes liquid fermentation process with above-mentioned streptomyces hygroscopicus liquid.
Embodiment 1:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that dry Nanguo Pear waste residue obtains, and sterilized water 53.2 parts, mixes, obtained mixed solution.Again streptomyces hygroscopicus liquid 2.5 parts, Chinese cassia tree brown streptomycete liquid 2.5 parts, bacillus amyloliquefaciens liquid 3 parts, Bacillus sphaericus liquid 3 parts, bacillusmusilaginosiengineering liquid 11 parts, blown-ball Azotobacter liquid 2.4 parts, golden streptomyces chromogenes liquid 2.4 parts are mixed with above-mentioned mixed solution, obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For wheat paddock, spray plant by 1:300 ~ 500 times diluent, 300 ~ 500mL is used in every mu of ground at every turn.Sprinkling period can in nursery, setting date, initial bloom stage, productive phase, sprays once every about 7 days, uses 2 ~ 3 times continuously, receive positive effect, plays the fertile double effects of medicine.
Embodiment 2:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that dry Nanguo Pear waste residue obtains, and sterilized water 47 parts, mixes, obtained mixed solution.Again by streptomyces hygroscopicus liquid 3 parts, Chinese cassia tree brown streptomycete liquid 3 parts, bacillus amyloliquefaciens liquid 4 parts, Bacillus sphaericus liquid 4 parts, bacillusmusilaginosiengineering liquid 13 parts, blown-ball Azotobacter liquid 3 parts, golden streptomyces chromogenes liquid 3 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio, for rice terrace, this product 0.5kg is used to soak rice paddy seed 2.5kg7 days, 25 ~ 30 DEG C of vernalization sowings, play the effect of fertile medicine economic benefits and social benefits.
Embodiment 3:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that dry Nanguo Pear waste residue obtains, and sterilized water 59 parts, mixes, obtained mixed solution.Again by streptomyces hygroscopicus liquid 2 parts, Chinese cassia tree brown streptomycete liquid 2 parts, bacillus amyloliquefaciens liquid 2 parts, Bacillus sphaericus liquid 2 parts, bacillusmusilaginosiengineering liquid 9 parts, blown-ball Azotobacter liquid 2 parts, golden streptomyces chromogenes liquid 2 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count about 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For corn field, spray plant by 1:300 ~ 500 times diluent, this product 300 ~ 500mL is used on every mu of ground at every turn.Sprinkling period can in nursery, setting date, initial bloom stage, productive phase, sprays once every about 7 days, uses 2 ~ 3 times continuously, receive positive effect, plays the fertile double effects of medicine.
Embodiment 4:
Solid medium is that dried thin mushroom bacterium discards rod obtained trichoderma viride fermentative degradation liquid 10 parts, matrix 10 parts, and sterilized water 53.2 parts, mixes, obtained mixed solution.Again streptomyces hygroscopicus liquid 2.5 parts, Chinese cassia tree brown streptomycete liquid 2.5 parts, bacillus amyloliquefaciens liquid 3 parts, Bacillus sphaericus liquid 3 parts, bacillusmusilaginosiengineering liquid 11 parts, blown-ball Azotobacter liquid 2.4 parts, golden streptomyces chromogenes liquid 2.4 parts are mixed with above-mentioned mixed solution, obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For wheat paddock, spray plant by 1:300 ~ 500 times diluent, 300 ~ 500mL is used in every mu of ground at every turn.Sprinkling period is nursery, setting date, and initial bloom stage, productive phase, sprayed once every about 7 days, uses 2-3 time continuously, receives positive effect, plays medicine fertilizer double effects.
Embodiment 5:
Solid medium is that dried thin mushroom bacterium discards rod obtained trichoderma viride fermentative degradation liquid 10 parts, matrix 10 parts, and sterilized water 47 parts, mixes, obtained mixed solution.Again by streptomyces hygroscopicus liquid 3 parts, Chinese cassia tree brown streptomycete liquid 3 parts, bacillus amyloliquefaciens liquid 4 parts, Bacillus sphaericus liquid 4 parts, bacillusmusilaginosiengineering liquid 13 parts, blown-ball Azotobacter liquid 3 parts, golden streptomyces chromogenes liquid 3 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For rice terrace, use this product 0.5kg to soak rice paddy seed 2.5kg7 days, sow after 25 ~ 30 DEG C of vernalization, play the effect of fertile medicine economic benefits and social benefits.
Embodiment 6:
Solid medium is that dried thin mushroom bacterium discards rod obtained trichoderma viride fermentative degradation liquid 10 parts, matrix 10 parts, and sterilized water 59 parts, mixes, obtained mixed solution.Again by streptomyces hygroscopicus liquid 2 parts, Chinese cassia tree brown streptomycete liquid 2 parts, bacillus amyloliquefaciens liquid 2 parts, Bacillus sphaericus liquid 2 parts, bacillusmusilaginosiengineering liquid 9 parts, blown-ball Azotobacter liquid 2 parts, golden streptomyces chromogenes liquid 2 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count about 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For corn field, spray plant by 1:300 ~ 500 times diluent, 300 ~ 500mL is used in every mu of ground at every turn.Sprinkling period is nursery, setting date, and initial bloom stage, productive phase, sprayed once every about 7 days, use 2 ~ 3 times continuously, receive positive effect, plays the fertile double effects of medicine.
Embodiment 7:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that solid carbon dioxide rice straw obtains, and sterilized water 53.2 parts, mixes, obtained mixed solution.Again streptomyces hygroscopicus liquid 2.5 parts, Chinese cassia tree brown streptomycete liquid 2.5 parts, bacillus amyloliquefaciens liquid 3 parts, Bacillus sphaericus liquid 3 parts, bacillusmusilaginosiengineering liquid 11 parts, blown-ball Azotobacter liquid 2.4 parts, golden streptomyces chromogenes liquid 2.4 parts are mixed with above-mentioned mixed solution, obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For wheat paddock, spray plant by 1:300-500 times of diluent, 300 ~ 500mL is used in every mu of ground at every turn.Sprinkling period is nursery, setting date, and initial bloom stage, productive phase, sprayed once every about 7 days, use 2 ~ 3 times continuously, receive positive effect, plays the fertile double effects of medicine.
Embodiment 8:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that solid carbon dioxide rice straw obtains, and sterilized water 47 parts, mixes, obtained mixed solution.Again by streptomyces hygroscopicus liquid 3 parts, Chinese cassia tree brown streptomycete liquid 3 parts, bacillus amyloliquefaciens liquid 4 parts, Bacillus sphaericus liquid 4 parts, bacillusmusilaginosiengineering liquid 13 parts, blown-ball Azotobacter liquid 3 parts, golden streptomyces chromogenes liquid 3 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count be greater than 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio, for rice terrace, this product 0.5kg is used to soak rice paddy seed 2.5kg7 days, 25 ~ 30 DEG C of vernalization can be sowed, and play the effect of fertile medicine economic benefits and social benefits.
Embodiment 9:
Solid medium is trichoderma viride fermentative degradation liquid 10 parts, the matrix 10 parts that solid carbon dioxide rice straw obtains, and sterilized water 59 parts, mixes, obtained mixed solution.
Again by streptomyces hygroscopicus liquid 2 parts, Chinese cassia tree brown streptomycete liquid 2 parts, bacillus amyloliquefaciens liquid 2 parts, Bacillus sphaericus liquid 2 parts, bacillusmusilaginosiengineering liquid 9 parts, blown-ball Azotobacter liquid 2 parts, golden streptomyces chromogenes liquid 2 parts, mix with above-mentioned mixed solution, can obtain containing living bacteria count about 2.0 hundred million/gram the multi-control fertilizer of liquid compound bio.For corn field, spray plant by 1:300-500 times of diluent, 300 ~ 500mL is used in every mu of ground at every turn.Sprinkling period is nursery, setting date, and initial bloom stage, productive phase, sprayed once every about 7 days, use 2 ~ 3 times continuously, receive positive effect, plays the fertile double effects of medicine.
Comparative example 1: the composite microbiological fertilizer produced for Bao Nong bio tech ltd, Hong Kong.
Comparative example 2: be the Enteromorpha bio-bacterial manure of producing with Qingdao Seawin Biotech Group Co., Ltd..
Comparative example 3: the composite bacteria seed manure be combined with Taiwan promise Ka Shi actinomycetes for Japanese silicate bacteria.
The multi-control fertilizer of compound bio of the embodiment of the present invention 1 ~ 9 is as follows through the main experimental conditions of field comparison test, method and result:
Rice test: in hole village, Chang Mount town, Donggang City, excellent No. one of kind New Port, diagonal lines 5 sampling, often some 1m
2, carry out actual measurement, calculate rice yield by point, 300 times of sprayings+200 times of seed soaking 7 days; Control group CK district.
Corn trials: in experimental plot, Yao Tun village, Datun Town, Haicheng City, diagonal lines 5 sampling, often 40 strains, in some product, are sprayed+200 times for 300 times and are soaked seed 5 days; Control group CK district.
Wheat is tested: in experimental plot, Hebei Jizhou City, diagonal lines 5 sampling, and often 40 strains, in some product, are sprayed+200 times for 300 times and soaked seed 5 days; Control group CK district.
Capsicum, eggplant, tomato test: diagonal lines 5 sampling, often 6 strains, in some product, are sprayed+200 times for 300 times and are soaked seed 5 days; Control group CK district.
Kyoto grape is tested: in great Tun vineyard, Haicheng City, the equidistant sampling of parallel lines, totally 5 points, and often 2 strains, in some product, are sprayed+200 times for 300 times and filled with roots 5 days; Control group CK district.
Table 1 embodiment and comparative example on soil layer construction, organic matter, soil pH affect situation:
The mean yield situation of the comparative example of table 2 embodiment and nutrient such as grade:
Table 3 embodiment and comparative example such as nutrient such as grade are to the mean yield situation of food crop:
Table 4 embodiment affects situation with waiting nutrient comparative example to food crop quality:
Table 5 embodiment and comparative example such as nutrient such as grade are to the qualitative effects situation of fruit trees and vegetables:
Embodiment 2,5 and 8 is applied to rice terrace, compares, make the crab of cultivation, loach, swamp eel net gain 8 ~ 12% with use common fertilizer.
The shelf life of products of the embodiment of the present invention is 12 ~ 18 months.
Claims (4)
1. the multi-control fertilizer of liquid compound bio, is characterized in that, it is made up of the feed composition of following weight:
Streptomyces hygroscopicus liquid 1 ~ 3 part, Chinese cassia tree brown streptomycete liquid 1 ~ 3 part, bacillus amyloliquefaciens liquid 2 ~ 4 parts, Bacillus sphaericus liquid 2 ~ 4 parts, bacillusmusilaginosiengineering liquid 7 ~ 15 parts, blown-ball Azotobacter liquid 1 ~ 3 part, golden streptomyces chromogenes liquid 1 ~ 3 part, the fermentative degradation liquid 8 ~ 10 parts of trichoderma viride, matrix 8 ~ 10 parts and sterilized water 45 ~ 65 parts
Wherein, the fermentation process of the fermentative degradation liquid of trichoderma viride is by weight ratio, is inoculated in by 3.6 parts of viride bacterial classifications on 15 parts of solid mediums, water content is made to be 60%, culture temperature 25 ~ 27 DEG C, pH value 7.0, at incubation time 56 ~ 72 hours 121 DEG C, sterilizing 20 minutes, to obtain final product;
Solid medium is discarded after rod is pulverized by edible fruits waste residue, straw or edible mushrooms and is prepared, edible fruits waste residue refers to the fruit waste residue of Nanguo Pear, white pear or apple, straw refers to the stalk of paddy rice, wheat, Chinese sorghum or corn, and edible mushrooms discards the discarded rod of edible mushrooms that rod refers to mushroom, Pleurotus eryngii or Coprinus comatus;
Matrix is water-soluble humic acid or the one of becoming thoroughly decomposed in feces of livestock and poultry water or two kinds of mixtures;
The feces of livestock and poultry water that becomes thoroughly decomposed refers to the ight soil water that becomes thoroughly decomposed of ox, horse or sheep class animal and chicken, duck or bird class;
Sterilized water refers to that seawater and freshwater is the water of 1:180 ~ 220 mixing and sterilizing according to weight proportion.
2. the multi-control fertilizer of liquid compound bio according to claim 1, is characterized in that, it is made up of the feed composition of following weight:
Streptomyces hygroscopicus liquid 2 ~ 3 parts, Chinese cassia tree brown streptomycete liquid 2 ~ 3 parts, bacillus amyloliquefaciens liquid 2 ~ 4 parts, Bacillus sphaericus liquid 2 ~ 4 parts, bacillusmusilaginosiengineering liquid 9 ~ 13 parts, blown-ball Azotobacter liquid 2 ~ 3 parts, golden streptomyces chromogenes liquid 2 ~ 3 parts, the fermentative degradation liquid 9 ~ 10 parts of trichoderma viride, matrix 9 ~ 10 parts and sterilized water 47 ~ 61 parts
Wherein, the fermentation process of the degradation solution of trichoderma viride is with described in claim 1;
Matrix be water-soluble humic acid and Chicken dung just water weight proportion be the mixture of 1:1 ~ 1.2;
Sterilized water refers to that seawater and freshwater is that 1:190 ~ 210 mix according to weight proportion, the sterilizing water of 20 minutes at 121 DEG C.
3. the purposes of the multi-control fertilizer of liquid compound bio according to claim 1, be applied to paddy rice, corn, wheat and fruits and vegetables seed soaking, fill with root or foliage-spray.
4. the preparation method of the multi-control fertilizer of liquid compound bio according to claim 1, it is characterized in that, its preparation process is as follows:
The preparation of A, trichoderma viride fermentative degradation liquid:
By weight ratio, take 15 parts of solid mediums, load in fermentation vat, add 9 parts of water, water content is made to reach 60%, pH value 7.0, stir, mix, sterilizing 20 minutes at 121 DEG C, be down to room temperature, again 3.6 parts of viride bacterial classifications are inoculated in solid medium top fermentation, culture temperature 25 ~ 27 DEG C, incubation time 56 ~ 72 hours, for keeping being uniformly distributed of moisture and moisture in fermenting process, adopt within every two days, in solid medium, spray into a small amount of aqua sterilisa, water content is made to keep 60%, and stirred, to make fermentation complete, in order to ensure the ventilation in fermentation, four layers of Breathable nonwoven are adopted to cover Chi Kou, the degradation product fermented sterilizing 20 minutes after filtration and at 121 DEG C, obtain,
B, various feed composition are prepared burden by its weight:
Obtained trichoderma viride fermentative degradation liquid is mixed with described matrix and sterilized water, add streptomyces hygroscopicus liquid, Chinese cassia tree brown streptomycete liquid, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid and golden streptomyces chromogenes liquid again, mix, obtain the multi-control fertilizer of liquid compound bio;
Wherein the weight of feed composition is with claim 1.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721345A (en) * | 2001-01-23 | 2006-01-18 | 任啟刚 | Feces and urine treatment method by compound microbe |
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN101357857A (en) * | 2008-09-05 | 2009-02-04 | 辽宁科技大学 | Bio organic fertilizer for aquaculture and paddy field and producing method thereof |
| CN102718603A (en) * | 2012-07-11 | 2012-10-10 | 德州市元和农业科技开发有限责任公司 | Compound microbial fertilizer and preparation method thereof |
-
2015
- 2015-01-11 CN CN201510013030.0A patent/CN104496707B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721345A (en) * | 2001-01-23 | 2006-01-18 | 任啟刚 | Feces and urine treatment method by compound microbe |
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN101357857A (en) * | 2008-09-05 | 2009-02-04 | 辽宁科技大学 | Bio organic fertilizer for aquaculture and paddy field and producing method thereof |
| CN102718603A (en) * | 2012-07-11 | 2012-10-10 | 德州市元和农业科技开发有限责任公司 | Compound microbial fertilizer and preparation method thereof |
Cited By (20)
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