CN105693293A - Multifunctional microbial fertilizer and preparation method thereof - Google Patents
Multifunctional microbial fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN105693293A CN105693293A CN201610068970.4A CN201610068970A CN105693293A CN 105693293 A CN105693293 A CN 105693293A CN 201610068970 A CN201610068970 A CN 201610068970A CN 105693293 A CN105693293 A CN 105693293A
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- bacillus
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- fertilizer
- bacteria
- azotobacter chroococcum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a multifunctional microbial fertilizer and a preparation method thereof. The preparation method of the multifunctional microbial fertilizer comprises the following steps: preparing a culture medium; culturing azotobacter chroococcum, bacillus mucilaginosus, bacillus megaterium and bacillus subtilis in the culture medium until maturing, and then culturing for 20-24 hours, so as to obtain each bacterium solution; mixing the obtained bacterium solutions according to a volume ratio of (1-2) to (0.5-1) to (0.5-1) to (0.5-1), adding sterilizing turf for absorption, and carrying out solid fermentation for 18-24 hours; and after the solid fermentation is finished, grinding. According to the multifunctional microbial fertilizer, nitrogen-fixing bacteria are capable of automatically fixing nitrogen in air and converting nitrogen into necessary nitrogen fertilizers of crops; phosphorus decomposing bacteria and potassium decomposing bacteria are capable of decomposing great amounts of insoluble phosphate and potassium deposited in soil into a phosphate fertilizer and a potassium fertilizer which can be reabsorbed and reused by the crops; disease-resistant bacteria are capable of inhibiting bacteria, so that diseases can be reduced; furthermore, by virtue of microorganisms, heavy metals in soil can be reduced and purified, or the toxicities of the heavy metals can be reduced; and after the microbial fertilizer is applied to the soil, beneficial microorganisms are increased, and harmful microorganisms are obviously reduced.
Description
One, technical field
The invention belongs to bio-fertilizer technical field, be specifically related to a kind of multifunctional microbial bacterial manure and preparation method thereof。
Two, background technology
At present, domestic microbiological fertilizer mainly has following several: (1) root nodule bacteria fertilizer；(2) nitrogen-fixing bacteria fertilizer；(3) Activation inoculant of soil phosphous；(4) Silicate Bacteria Fertilizer；(5) compound microbial fertilizer etc.。Above-mentioned several fertilizer not only fertilizer efficiency is single, and the problem that all can not solve heavy metal pollution of soil, it is difficult to practical requirement。
Three, summary of the invention
It is an object of the invention to provide a kind of multifunctional microbial bacterial manure and preparation method thereof, adopt this multifunctional microbial bacterial manure not only fertilizer efficiency high, moreover it is possible to effectively solve the problems such as heavy metal pollution of soil。
For achieving the above object, the technical solution used in the present invention is:
One, multifunctional microbial bacterial manure of the present invention is prepared by following preparation method。
Two, the preparation method of multifunctional microbial bacterial manure of the present invention, comprises the steps:
1. azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis are weighed
Bacterium desired nutritional element, prepares respective culture medium；
2. after being placed in azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis in different seed tank to utilize respective culture medium culturing maturation, then go to and in respective fermentation tank, cultivate 20 24 hours prepared azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution；
3. after gained azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution being mixed according to the volume ratio of 1-2:0.5-1:0.5-1:0.5-1, according to the ratio of every 23 kilograms of mattress peats composed of rotten mosses that go out of 10 liters of mixed bacteria liquids, add the death of monks or nuns mattress peat composed of rotten mosses and carry out absorption also solid fermentation 18 24 hours；
4., after solid fermentation terminates, measure products therefrom water content and be crushed to when 25-35% and loose obtain without granule。
Above-mentioned azotobacter chroococcum (Azotobactechroococcum), colloid bacillus cereus (Bacillusmucilaginosus), bacillus megaterium (Bacillusmegatherium), bacillus subtilis (Bacillussubtilis) are existing conventional bacterium, are all buied from National agricultural portion Culture Collection or Chinese Academy of Sciences's Shenyang ecological Studies。
Multifunctional microbial bacterial manure of the present invention is with the high-quality sterilizing peat composed of rotten mosses for carrier, and every gram, rich in active effective bacteria 2-3 more than hundred million, is commonly called as fertilizer of living。Nitrogen-fixing bacteria azotobacter chroococcum can fix the nitrogen in air automatically, is converted into the nitrogenous fertilizer that crops are required。Phosphate solubilizing bacteria and potassium solubilizing bacteria bacillus megaterium and bacillus subtilis, the insolubility phosphorus potassium that can will be deposited in a large number in soil, resolving into crops can the phosphate fertilizer that utilizes of re-absorption and potash fertilizer。Anti-bacterium bacillus subtilis can make pathogenic bacteria be suppressed, and can reduce the generation of disease；It is also with making beneficial microbe increase after microorganism, reduction, purification heavy metal in soil or reduction heavy metal toxicity, and microbial manure are manured into soil, harmful microorganism significantly reduces, simultaneously as the activity of microorganism, making Soil structure improve, fertility is improved。
Multifunctional microbial bacterial manure of the present invention has the advantage that
(1) function is strong, it is wide to adapt to: suitable in cereal crops, industrial crops, forest flower and grass class。
(2) nutrition is complete, function is many: can fixing, discharge the nitrogen contained by various common fertilizer, phosphorus, potassium three elements, again containing various trace element, the secondary metabolites of microorganism also has biological regulation function, moreover it is possible to passivation heavy metal-polluted soil。
(3) consumption is few, high efficiency: mu is with 23 kilograms, and namely mu puts into 10-20 unit, reduces 30-50% fertilizer amount, can increase production 10-30%, and particular plants (such as vegetable) increases production more。
(4) easily use, method many: can mix with fertilizer do base manure or preparation seedling nutritious soil, minimizing seed manure consumption of can dressing seed replaces the seed dressing of antibacterial, coating materials, can be used for vegetable transplant seedlings and broadcast that seedlings plugging wood dips in root, suitable greenhouse crops, melon and fruit pouring root are topdressed, various crops all can foliage-spray。
(5) subtract disease, urge precocity: strengthen crop resistance against disadvantage, suppress the soil-borne disease bacterium procreations such as Fusarium spp., can prevent, alleviate the disease generations such as Fusarium and Verticillium wilts, cucurbits fusarium wilt, downy mildew。And most crop can be made to shift to an earlier date 3-5 days maturations。
(6) quality better, without residual hazard: can improve melon and fruit, sugariness content 10-30%, produce vegetables good。Cereal crops protein, amino acid content can be improved, be produce desirable fertilizer green, Organic food。
(7) improve the soil earth, training soil fertility: continuous administration 3-5, and the soil that can make to harden becomes loose, organic increases, and soil physical and chemical property improves。
Four, detailed description of the invention
Embodiment 1:
1. azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis are weighed
Bacterium desired nutritional element prepares respective culture medium；
2., after being placed in azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis in different seed tank to utilize respective culture medium culturing maturation, go to cultivate in respective fermentation tank 20 24 hours and prepare azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution respectively；
3. after gained azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution being mixed according to the volume ratio of 1:1:1:1, according to the ratio of every 23 kilograms of mattress peats composed of rotten mosses that go out of 10 liters of mixed bacteria liquids, add the death of monks or nuns mattress peat composed of rotten mosses and carry out adsorbing and go to solid fermentation 18 24 hours in fermentation tank；
4., after solid fermentation terminates, measure products therefrom water content when 25-35%, be crushed to pulverizer loose without granule, through quantitative package after the assay was approved。
Embodiment 2:
1. azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis are weighed
Bacterium desired nutritional element prepares respective culture medium；
2., after being placed in azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis in different seed tank to utilize respective culture medium culturing maturation, go to cultivate in respective fermentation tank 20 24 hours and prepare azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution respectively；
3. after gained azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution being mixed according to the volume ratio of 2:1:1:1, according to the ratio of every 23 kilograms of mattress peats composed of rotten mosses that go out of 10 liters of mixed bacteria liquids, add the death of monks or nuns mattress peat composed of rotten mosses and carry out adsorbing and go to solid fermentation 18 24 hours in fermentation tank；
4., after solid fermentation terminates, measure products therefrom water content when 25-35%, be crushed to pulverizer loose without granule, through quantitative package after the assay was approved。
Embodiment 3:
1. azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis are weighed
Bacterium desired nutritional element prepares respective culture medium；
2., after being placed in azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis in different seed tank to utilize respective culture medium culturing maturation, go to cultivate in respective fermentation tank 20 24 hours and prepare azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution respectively；
3. after gained azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution being mixed according to the volume ratio of 2:0.5:0.5:1, according to the ratio of every 23 kilograms of mattress peats composed of rotten mosses that go out of 10 liters of mixed bacteria liquids, add the death of monks or nuns mattress peat composed of rotten mosses and carry out adsorbing and go to solid fermentation 18 24 hours in fermentation tank；
4., after solid fermentation terminates, measure products therefrom water content when 25-35%, be crushed to pulverizer loose without granule, through quantitative package after the assay was approved。
Claims (2)
1. a preparation method for multifunctional microbial bacterial manure, comprises the steps:
1. weigh azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis desired nutritional element, prepare respective culture medium；
2. after being placed in azotobacter chroococcum, colloid bacillus cereus, bacillus megaterium, bacillus subtilis in different seed tank to utilize respective culture medium culturing maturation, then go to and in respective fermentation tank, cultivate 20 24 hours prepared azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution；
3. after gained azotobacter chroococcum liquid, colloid bacillus cereus liquid, bacillus megaterium liquid, bacillus subtilis bacterium solution being mixed according to the volume ratio of 1-2:0.5-1:0.5-1:0.5-1, according to the ratio of every 23 kilograms of mattress peats composed of rotten mosses that go out of 10 liters of mixed bacteria liquids, add the death of monks or nuns mattress peat composed of rotten mosses and carry out absorption also solid fermentation 18 24 hours；
4., after solid fermentation terminates, measure products therefrom water content and be crushed to when 25-35% and loose obtain without granule。
2. the multifunctional microbial bacterial manure that prepared by a method as claimed in claim 1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610068970.4A CN105693293A (en) | 2016-02-02 | 2016-02-02 | Multifunctional microbial fertilizer and preparation method thereof |
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| CN201610068970.4A CN105693293A (en) | 2016-02-02 | 2016-02-02 | Multifunctional microbial fertilizer and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748042A (en) * | 2016-12-05 | 2017-05-31 | 湖南农业大学 | Biological organic fertilizer leavening and preparation method thereof |
| CN106811433A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect |
| CN107056491A (en) * | 2017-05-27 | 2017-08-18 | 明光市裕阳农业有限公司 | A kind of composite microbic bacterial fertilizer and preparation method thereof |
| CN108503482A (en) * | 2018-04-04 | 2018-09-07 | 上海绿博生物科技发展有限公司 | A kind of effective biological agent and preparation method thereof for promoting rice yield |
| CN116217290A (en) * | 2023-01-31 | 2023-06-06 | 石家庄中农兴泰生物科技有限公司 | Efficient phosphate-dissolving compound microbial fertilizer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331061A (en) * | 2000-06-30 | 2002-01-16 | 李良发 | Microbe fertilizer with nitrogen-fixating and P and K decomposing functions |
| CN103896642A (en) * | 2012-12-25 | 2014-07-02 | 青岛凯利尔生物科技有限公司 | Microbial bacterial fertilizer |
| CN104496707A (en) * | 2015-01-11 | 2015-04-08 | 鞍山禾瑞生物科技有限公司 | Liquid composite biological multi-control fertilizer, and preparation method and application thereof |
-
2016
- 2016-02-02 CN CN201610068970.4A patent/CN105693293A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331061A (en) * | 2000-06-30 | 2002-01-16 | 李良发 | Microbe fertilizer with nitrogen-fixating and P and K decomposing functions |
| CN103896642A (en) * | 2012-12-25 | 2014-07-02 | 青岛凯利尔生物科技有限公司 | Microbial bacterial fertilizer |
| CN104496707A (en) * | 2015-01-11 | 2015-04-08 | 鞍山禾瑞生物科技有限公司 | Liquid composite biological multi-control fertilizer, and preparation method and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748042A (en) * | 2016-12-05 | 2017-05-31 | 湖南农业大学 | Biological organic fertilizer leavening and preparation method thereof |
| CN106748042B (en) * | 2016-12-05 | 2018-11-09 | 湖南农业大学 | Biological organic fertilizer leavening and preparation method thereof |
| CN106811433A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect |
| CN107056491A (en) * | 2017-05-27 | 2017-08-18 | 明光市裕阳农业有限公司 | A kind of composite microbic bacterial fertilizer and preparation method thereof |
| CN108503482A (en) * | 2018-04-04 | 2018-09-07 | 上海绿博生物科技发展有限公司 | A kind of effective biological agent and preparation method thereof for promoting rice yield |
| CN116217290A (en) * | 2023-01-31 | 2023-06-06 | 石家庄中农兴泰生物科技有限公司 | Efficient phosphate-dissolving compound microbial fertilizer and preparation method thereof |
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Application publication date: 20160622 |
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