CN106748042B - Biological organic fertilizer leavening and preparation method thereof - Google Patents

Biological organic fertilizer leavening and preparation method thereof Download PDF

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Publication number
CN106748042B
CN106748042B CN201611101754.1A CN201611101754A CN106748042B CN 106748042 B CN106748042 B CN 106748042B CN 201611101754 A CN201611101754 A CN 201611101754A CN 106748042 B CN106748042 B CN 106748042B
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Prior art keywords
powder
bacillus subtilis
bacillus
leavening
plant growth
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CN106748042A (en
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兰时乐
王惠群
邓元元
易自力
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Inner Mongolia Ruixiang Agriculture and Animal Husbandry Development Co., Ltd.
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Hunan Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

A kind of biological organic fertilizer leavening and preparation method thereof, which is to take bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder and colloid bacillus cereus bacterium powder, is uniformly mixed;It will be after mixing added in the bacterium powder of aforementioned mixing, stirred evenly to get total bacteria count >=10 in leavening obtained with the dissolved plant growth regulator of ethyl alcohol and filler again10CFU/g.The present invention uses composite flora, utilize aerobic composting fermentation technology, the specific functionality advantage of each bacterium can not only be played, in addition adding microbial growth promoters, the degradation rate of organic matter can be greatlyd improve, makes organic matter promptly degradation and conversion under the action of complex microorganism, and the auxin for promoting plant growth in fertilizer can be increased, it is simple with preparation process, stable and reliable product quality, it is of low cost the features such as.

Description

Biological organic fertilizer leavening and preparation method thereof
Technical field
The invention belongs to agricultural biological technical fields, are related to a kind of biological organic fertilizer leavening, while being related to the leavening Preparation method.
Background technology
Soil is the basis of agricultural, and fertilizer is the grain of crops, and China is maximum large agricultural country in the world, is needed every year It is the fertilizer of the nutrient needed containing one or more of crop growths to want a large amount of fertilizer, fertilizer.Needed for plant growth The grand nutrition element wanted has carbon, hydrogen, oxygen, nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, micronutrient element have boron, copper, iron, manganese, molybdenum, zinc, Chlorine etc..The use of fertilizer has greatly pushed the development of agricultural production, greatly improves the yield of crop, is passed through to its people The development and promotion agricultural volume increase of Ji have a very important significance.With the development of society and the improvement of people's living standards, Pollution-free food, pollution-free food and organic food are increasingly subject to the people's attention.Due to chemical fertilizer and chemical pesticide It is long-term a large amount of to use, while increasing crop yield, under also resulting in soil organic matter content, microbe species and quantity drastically Drop, agroecological environment are seriously damaged, and quality of agricultural product declines.For defect existing for inorganic fertilizer and with people couple The pay attention to day by day of health, food security etc., it is higher and higher to the quality requirements of agricultural product so that organic fertilizer receives blueness It looks at.
Biological organic fertilizer refer to microorganisms with specific functions with mainly with plant and animal residues(Such as feces of livestock and poultry, agricultural crop straw Deng)The one kind being combined for source and through harmless treatment, decomposed organic materials has both microbial manure and organic fertilizer efficiency The fertilizer answered.Its main function is to be formed mutually with Soil Microorganism after the beneficial microbe in biological organic fertilizer enters soil Between symbiosis Relationship With Proliferation, inhibit harmful bacteria grow and be converted into beneficial bacterium, interact, mutually promote, play the association of group Same-action, beneficial bacterium generate a large amount of metabolite during growth and breeding, promote the decomposition and inversion of organic matter, can directly or Between be connected in crop a variety of nutrition and pungent be provided, promote and regulation and control plant growth.
Organic fertilizer fermentation agent be by be capable of the microorganism group of decomposing protein, cellulose, hemicellulose, lignin etc. at. The living bacteria count content of organic fertilizer fermentation agent is high, and degradation capability is strong, while can reach heating, deodorization, eliminate pest and disease damage, is miscellaneous Grass seed and the effect for improving nutrient.Under appropriate conditions, can carbon, nitrogen, phosphorus, potassium, the sulphur etc. in windrow be decomposed into mine rapidly Change, simple organic is formed, to be further broken into the absorbable nutritional ingredient of crop.
Generally existing " triple three is light " phenomenon, i.e., heavy chemical fertilizer, light organic fertilizer, diazonium phosphate fertilizer, light potassium currently, peasant applies fertilizer Fertilizer, great secondary element are fertile, light middle micro-.Develop organic fertilizer, is exactly to utilize itself raw material advantage, by different crops Fertile feature and agricultural production requirement are needed, the exploitation of organic fertilizer depth is carried out, it is suitable with the fertilizer such as chemical fertilizer, biological inoculum and middle trace element Amount proportioning, balance application promote agricultural production high yield, high-quality, efficient to improve fertilizer nutrient utilization.Domestic patent CN105753601A, CN104529576A, CN103664260A, CN104862298A, CN1500862A etc. disclose organic fertilizer Leavening and preparation method thereof, but its used main bacteria seed be genus lactubacillus, Blastocystis, bacillus, aspergillus, Some in actinomyces, azotobacter, photosynthetic bacteria etc., some is also added with cellulase, protease and phosphorylase etc..By Gemma is not generated in some of which kind, can be killed due to high temperature during organic fertilizer fermentation, not had microorganism and answer Play the role of;Various enzymes cause to inactivate due to the high temperature that fermentation generates simultaneously, can not play bio-enzyme degradation macromolecular The effect of substance, and there are the starter culture time-to-live is short, ferment effect is difficult to stablize, production technology complexity and is produced into The shortcomings of this is high.
Invention content
The technical problem to be solved by the present invention is to:In view of the above shortcomings of the prior art, a kind of biological organic fertilizer is provided Leavening and preparation method thereof, leavening preparation process is simple, cost and low energy consumption, adaptable, the preservation time is long, is easy to Batch production, ferment effect is stable and effect is good.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is:A kind of biological organic fertilizer leavening, Feature is:The leavening is by weight percentage by bacillus subtilis powder 35-50%, Bacillus subtilis subspecies bacterium powder 15- 20%, bacillus megaterium powder 15-20%, colloid bacillus cereus powder 12-20%, plant growth regulator 0.2-0.5% and filler 5-10% is formed, total living bacteria count >=10 of the leavening10CFU/g;Wherein, above-mentioned plant growth regulator is 6- benzyl amino Purine.
Present invention simultaneously provides the preparation methods of above-mentioned biological organic fertilizer leavening, and steps are as follows for this method:
(1)Bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder and glue are weighed respectively Matter bacillus bacterium powder is uniformly mixed;
(2)Plant growth regulator is weighed, is uniformly mixed with filler after fully being dissolved with the ethyl alcohol of 50% volumetric concentration;
(3)By step(1)And step(2)Two kinds of mixtures mixing, stir evenly.
The ethyl alcohol of above-mentioned 50% volumetric concentration referred to, is not one of constituent of this leavening, will not be with this hair Bacterium powder in ferment agent reacts, and is only used as dissolving plant growth regulator, and dosage does not limit, as long as can be fully molten Plant growth regulator is solved, preferably, the ethyl alcohol of 3000-5000mL can be used.
Above-mentioned filler mixes for one or both of bamboo charcoal powder or husk charcoal.
The bacillus subtilis is the bacillus subtilis that can generate the class basic element of cell division, auxin substance.
The Bacillus subtilis subspecies are to generate cellulase and the bacillus subtilis of hemicellulase is withered Careless subspecies.
Strain used in the present invention is the prior art, and preserving number is respectively:Bacillus subtilis(Bacillus subtilis):CICC20872, ACCC19743 or CGMCC3038;Bacillus megaterium(Bacillus megaterium): CICC21580, CGMCC8802 or ACCC02991;Colloid bacillus cereus(Bacillus mucilaginosus):ACCC 10012, LICC10201 or CGMCC5766;Bacillus subtilis subspecies(Bacillus subtilis subsp.subtilis):CGMCC 1.3358, ACCC 19374 or GIM1.372.
Compared with prior art, the present invention has the following advantages:
(1)Microorganism fungus kind used by the method for the present invention in addition to dissolving phosphor and dissolving potassium effect bacillus megaterium and glue Outside matter bacillus, also contain can generate the class basic element of cell division, auxin substance bacillus subtilis and can produce The Bacillus subtilis subspecies of raw cellulase and hemicellulase, can not only fast degradation organic matter, decompose in soil Fixed phosphorus and potassium, and the class basic element of cell division and growth hormone of stimulation plant growth can be generated.
(2)The method of the present invention compared with existing production technology, have simple production process, low energy consumption, product quality is stable, The features such as using effect is stablized.
Specific implementation mode
The invention will be further described for following embodiment.
Embodiment 1
This leavening forms(By weight percentage):Bacillus subtilis powder 35%, Bacillus subtilis subspecies bacterium powder 20%, bacillus megaterium powder 18%, colloid bacillus cereus powder 16.5%, 6-benzyl aminopurine 0.5%, husk charcoal 5% and bamboo charcoal powder 5%。
Preparation process:(1)By bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder And colloid bacillus cereus powder is placed in blender and stirs evenly;
(2)6-benzyl aminopurine is fully dissolved with the ethyl alcohol of 50% volumetric concentrations of 5000mL, then with husk charcoal and bamboo charcoal Powder is uniformly mixed;
(3)By step(1)With(2)Two kinds of mixtures mixing, stir evenly, obtain the present invention biological organic fertilizer hair Ferment agent;Total effectively bacterium number is 2.93 × 10 in the leavening10CFU/g。
Embodiment 2
This leavening forms(By weight percentage):Bacillus subtilis powder 50%, Bacillus subtilis subspecies bacterium powder 15%, bacillus megaterium powder 15%, colloid bacillus cereus powder 12%, 6-benzyl aminopurine 0.2%, bamboo charcoal powder 7.8%.
Preparation process:(1)By bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder And colloid bacillus cereus powder is placed in blender and stirs evenly;
(2)6-benzyl aminopurine is fully dissolved with the ethyl alcohol of 50% volumetric concentrations of 3000mL, then is mixed with bamboo charcoal powder It is even;
(3)By step(1)With(2)Two kinds of mixtures mixing, stir evenly, obtain the present invention biological organic fertilizer hair Ferment agent;Total effectively bacterium number is 3.47 × 10 in the leavening10CFU/g。
Embodiment 3
This leavening forms(By weight percentage):Bacillus subtilis powder 38%, Bacillus subtilis subspecies bacterium powder 16.7%, bacillus megaterium powder 20%, colloid bacillus cereus powder 20%, 6-benzyl aminopurine 0.3%, husk charcoal 5%.
Preparation process:(1)By bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder And colloid bacillus cereus powder is placed in blender and stirs evenly;
(2)6-benzyl aminopurine is fully dissolved with the ethyl alcohol of 50% volumetric concentrations of 4000mL, then is mixed with husk charcoal It is even;
(3)By step(1)With(2)Two kinds of mixtures mixing, stir evenly, obtain the present invention biological organic fertilizer hair Ferment agent;Total effectively bacterium number is 3.72 × 10 in the leavening10CFU/g。

Claims (2)

1. a kind of biological organic fertilizer leavening, which is characterized in that the leavening is by weight percentage by bacillus subtilis powder 35- 50%, Bacillus subtilis subspecies bacterium powder 15-20%, bacillus megaterium powder 15-20%, colloid bacillus cereus powder 12- 20%, plant growth regulator 0.2-0.5% and filler 5-10% compositions, total living bacteria count of the leavening >= 1010CFU/g;
Wherein, above-mentioned plant growth regulator is 6-benzyl aminopurine;
The bacillus subtilis is the bacillus subtilis that can generate the class basic element of cell division, auxin substance;It is selected from Preserving number is the bacillus subtilis of CICC20872, ACCC19743 or CGMCC3038;
The Bacillus subtilis subspecies are to generate the Bacillus subtilis Asia of cellulase and hemicellulase Kind, it is CGMCC 1.3358, ACCC 19374 or GIM1.372 Bacillus subtilis subspecies selected from preserving number;
Above-mentioned leavening is to be prepared as follows:
(1) bacillus subtilis powder, Bacillus subtilis subspecies bacterium powder, bacillus megaterium powder and colloid bud are weighed respectively Spore bacillus bacterium powder is uniformly mixed;
(2) plant growth regulator is weighed, is uniformly mixed with filler after fully being dissolved with the ethyl alcohol of 50% volumetric concentration;
(3) two kinds of mixtures of step (1) and step (2) are mixed, is stirred evenly.
2. biological organic fertilizer leavening as described in claim 1, which is characterized in that the filler is bamboo charcoal powder or husk charcoal One or both of mixing.
CN201611101754.1A 2016-12-05 2016-12-05 Biological organic fertilizer leavening and preparation method thereof Active CN106748042B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110818483A (en) * 2019-11-28 2020-02-21 江苏永佳现代农业科技有限公司 Root-promoting bio-organic fertilizer produced by treating agricultural solid waste and manufacturing method thereof
CN112457070A (en) * 2020-11-18 2021-03-09 上海玖沁环保科技有限公司 Environment-friendly biological agent for treating straws
CN113980834B (en) * 2021-09-23 2022-08-30 中化农业(临沂)研发中心有限公司 Bacillus subtilis and application thereof in fertilizer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101768021A (en) * 2008-12-31 2010-07-07 侯金荣 Organic material composting promoter and preparation method and application thereof
CN101928182A (en) * 2010-09-01 2010-12-29 山西晨雨科技开发连锁经营有限公司 Bio-organic fertilizer special for improving saline-alkali lands and preparation method thereof
CN105693293A (en) * 2016-02-02 2016-06-22 抚顺上马乡福来生物有机肥有限公司 Multifunctional microbial fertilizer and preparation method thereof
CN106083444A (en) * 2016-06-01 2016-11-09 大连三科生物工程有限公司 The preparation method of the organic composite fertilizer that a kind of Oryza sativa L. is special

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101768021A (en) * 2008-12-31 2010-07-07 侯金荣 Organic material composting promoter and preparation method and application thereof
CN101928182A (en) * 2010-09-01 2010-12-29 山西晨雨科技开发连锁经营有限公司 Bio-organic fertilizer special for improving saline-alkali lands and preparation method thereof
CN105693293A (en) * 2016-02-02 2016-06-22 抚顺上马乡福来生物有机肥有限公司 Multifunctional microbial fertilizer and preparation method thereof
CN106083444A (en) * 2016-06-01 2016-11-09 大连三科生物工程有限公司 The preparation method of the organic composite fertilizer that a kind of Oryza sativa L. is special

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Effective date of registration: 20190522

Address after: 137400 No. 209, Building 5, Ulanhada Town, Ulanhot City, Inner Mongolia Autonomous Region

Patentee after: Inner Mongolia Ruixiang Agriculture and Animal Husbandry Development Co., Ltd.

Address before: 410128 No. 1 Donghunong Road, Furong District, Changsha City, Hunan Province

Patentee before: Hunan Agricultural University