CN105777329B - A kind of solid union biology controls water conservation moisturizing fertilizer and the preparation method and application thereof - Google Patents
A kind of solid union biology controls water conservation moisturizing fertilizer and the preparation method and application thereof Download PDFInfo
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- CN105777329B CN105777329B CN201610080592.1A CN201610080592A CN105777329B CN 105777329 B CN105777329 B CN 105777329B CN 201610080592 A CN201610080592 A CN 201610080592A CN 105777329 B CN105777329 B CN 105777329B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Mechanical Engineering (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of solid union biology controls water conservation moisturizing fertilizer and its preparation method and application, and fertilizer material is made of bacterium solution, the bacterium solution of streptomyces microflavus, the bacterium solution of trichoderma viride of bentonite, matrix, the bacterium solution of streptomyces hygroscopicus, the bacterium solution of bacillus megaterium, the bacterium solution of bacillus amyloliquefaciens, the bacterium solution of Bacillus sphaericus, the bacterium solution of bacillusmusilaginosiengineering, the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, the bacterium solution of bacillus subtilis, golden streptomyces chromogenes；The preparation method comprises the following steps: (1) prepares bacterium solution；(2) each bacterium solution is mixed into drying with matrix, then with bentonite mixed pelletizing.Fertilizer of the invention has fixed nitrogen, phosphorus decomposing, Potassium release, absorbs water tariff collection soil moisture, promotes decomposition of soil organic matter, antibacterial pest-resistant, improvement Soil Microorganism group, improves the function of soil texture.
Description
Technical field
The present invention relates to technical field of biological fertilizer, in particular to the more control water conservation moisturizing fertilizer of a kind of solid union biology and
Preparation method and application.
Background technique
China's most area is in the mountain area hilly ground in arid and semi-arid lands, especially China nearly 70%, soil
Drought and barren, especially in the recent decade with global warming, rainfall falls sharply, Continuous Drought, in addition the over-extraction of underground water, ground
Table water is closely exhausted, and water resources crisis is on the rise.As Ningxia, China, the arid inland region in northwestern China at possession, 80% or more area
For arid, semiarid zone, drought, mean annual precipitation 289mm, water surface evaporation is up to 1210~1600mm, water
Resource is extremely short；Moreover the seasonality of crop growth is incompatible with precipitation in year, and agriculture seasonal drought ten is clearly demarcated
It is aobvious.In recent years, especially southwestern five provinces (area, city) have been recurred 2006~2007 years and 2009~2010 years especially big twice dry
The drought of drought, larger area causes massive losses to one's respective area industrial and agricultural production, while also giving living standard of urban and rural population water band
Extreme difficulties are carried out.Wherein, 2009~2010 years, autumn, winter, spring company's drought had occurred in southwestern five provinces (area, city) some areas in succession
Superposition, especially even more serious with Guizhou, Yunnan and some areas of Guangxi, wide, the influence journey of the length of arid duration, coverage
Degree is deep, is that history is rare.
Bentonite is nontoxic as a kind of water-retaining agent, releases water, water suction repeatedly, thus agriculturally people it is likened to it is " micro-
Type reservoir " can be widely used for agricultural, forestry, gardening.The major function of water-retaining agent is as follows: (1) be retained: water-retaining agent is not soluble in water,
But suitable own wt is can absorb into hundred times of water, and effectively inhibits moisture evaporation, slows down the speed of soil release water, reduces soil
The infiltration and loss of earth moisture, achieve the purpose that water conservation；(2) fertilizer conservation: water-retaining agent can be raw by crops such as fertilizer soluble in water
Nutriment required for long is fixed wherein, reduces the leaching loss of soluble nutrient to a certain extent, reaches fertilizer conservation
Effect；At the same time it can also play the role of efficiency slow release；(3) it keeps the temperature: after application water-retaining agent, being protected using the moisture of absorption
The Thermoregulation nocturnal temperature of part daylight generation is held, so that soil day and night temperature reduces；(4) improve soil texture: with
The regularity variation of water-retaining agent water swelling and dehydration shrinkage, surrounding soil can be made to become loose from consolidation, hole increases, from
And improve the penetrating situation of soil to a certain extent.It can be seen that the application of water-retaining agent is necessarily becoming for the future of agriculture development
Water-retaining agent and bio-feritlizer are especially combined application, had broad application prospects by gesture.Currently, Publication No.
Common organic fertilizer particles have been carried out Cotton seeds with water-retaining agent by the patent of invention of CN101054317A；Publication number
The patent of invention of CN101585737A uses pig manure for raw material, and polyacrylamide and deformation starch is added, water-retaining type has been made
Grain organic fertilizer；The patent of invention of publication number CN103058794A using the residual branch of mango, turf, feces of livestock and poultry, municipal refuse as raw material,
Biological organic fertilizer is made in addition agricultural water-loss reducer after decomposed.But it is more for corn, wheat, rice, herbage, the compound bio on lawn
The development or blank of control water conservation moisturizing fertilizer.
In addition, long-term largely can be such that the increase of soil inorganic salt content, organic matter reduces using chemical fertilizer, it is flat that soil loses soda acid
Weighing apparatus, soil property are hardened；And there is a serious shortage of lead to soil desertification to humic acid, moisture and the nutrient in soil.
Summary of the invention
The object of the present invention is to provide a kind of more controls of solid union biology to be retained moisturizing fertilizer and the preparation method and application thereof,
It using bentonite as water-retaining agent, is made with matrix and bacterium solution mixing granulation, the fertilizer of acquisition has biological pesticide, biology simultaneously
Fertilizer, biological nutrition and water conservation moisturizing quadruple effect.
The more control water conservation moisturizing fertilizer of solid union biology of the invention are made of the raw material components of following weight: swollen
1~10 part of profit soil, 40~90 parts of matrix, 1~3 part of the bacterium solution of streptomyces hygroscopicus, 1~3 part of the bacterium solution of bacillus megaterium, Xie Dian
2~4 parts of the bacterium solution of afnyloliquefaciens, 2~4 parts of the bacterium solution of Bacillus sphaericus, 7~15 parts of the bacterium solution of bacillusmusilaginosiengineering,
1~3 part of the bacterium solution of blown-ball Azotobacter, 1~3 part of the bacterium solution of bacillus cereus, 1~3 part of the bacterium solution of bacillus subtilis, gold produce
1~3 part of the bacterium solution of color streptomycete, 1~3 part of the bacterium solution of streptomyces microflavus, 8~10 parts of the bacterium solution of trichoderma viride.
Above-mentioned matrix is one or more of powdered rice hulls, humic acid, decomposed feces of livestock and poultry and straw powder, wherein rice husk
10~40 parts of powder, 10~40 parts of humic acid, 10~40 parts of decomposed feces of livestock and poultry, 10~40 parts of straw powder.
Above-mentioned decomposed feces of livestock and poultry selects the decomposed excrement of ox, horse or sheep class animal.
The preparation method of the more control water conservation moisturizing fertilizer of solid union biology of the invention sequentially includes the following steps:
1, bacterium solution is prepared: by streptomyces hygroscopicus, bacillus megaterium, bacillus amyloliquefaciens, Bacillus sphaericus, jelly
Sample bacillus, blown-ball Azotobacter, bacillus cereus, bacillus subtilis, golden streptomyces chromogenes, streptomyces microflavus and green
Using three-level, bacterium solution is respectively prepared in fermentation method to Trichoderma step by step, and preparation step includes slant strains activation culture, the expansion of tapered shaking flask
Big culture, one grade fermemtation tank liquid spread cultivation and second order fermentation tank spreads cultivation four steps；The bacterium solution, huge of streptomyces hygroscopicus is obtained respectively
The bacterium solutions of Bacterium anthracoides, the bacterium solution of bacillus amyloliquefaciens, the bacterium solution of Bacillus sphaericus, bacillusmusilaginosiengineering bacterium
Liquid, the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, the bacterium solution of bacillus subtilis, the bacterium solution of golden streptomyces chromogenes,
The bacterium solution of streptomyces microflavus and the bacterium solution of trichoderma viride；
2, by the bacterium solution of streptomyces hygroscopicus, the bacterium solution of bacillus megaterium, the bacterium solution of bacillus amyloliquefaciens, spherical gemma
The bacterium solution of bacillus, the bacterium solution of bacillusmusilaginosiengineering, the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, withered grass gemma
The bacterium solution of bacillus, bacterium solution, the bacterium solution of streptomyces microflavus, the bacterium solution of trichoderma viride of golden streptomyces chromogenes mix with matrix
It is even, then with bentonite mixing granulation, then moisture removal is removed in drying under the conditions of 40~50 DEG C, and the more controls of solid union biology are made and protect
Water moisturizing fertilizer.
In the above method, culture medium that the bacterium solution of trichoderma viride uses is prepared as PDA liquid medium, cultivation temperature 25
DEG C, pH is 7.0~7.2, and speed of agitator is 60~120 turns/min when culture, and the incubation time of each step is 48~72 hours.
In the above method, blown-ball Azotobacter, bacillus megaterium, bacillus amyloliquefaciens, Bacillus sphaericus, wax are prepared
The culture medium that the bacterium solution of sample bacillus, bacillus subtilis and bacillusmusilaginosiengineering uses is the training of beef extract-peptone liquid
Base is supported, 30 DEG C of cultivation temperature, pH is 7.2~7.8, and speed of agitator is 60~120 turns/min when culture, when the culture of each step
Between be 20~48 hours.
In the above method, the culture of the bacterium solution use of preparation streptomyces hygroscopicus liquid, golden streptomyces chromogenes and streptomyces microflavus
Base is Gause I fluid nutrient medium, and 28 DEG C of cultivation temperature, pH is 7.0~7.2, when culture speed of agitator be 60~120 turns/
Min, the incubation time of each step 120~144 hours.
The more control water conservation moisturizing fertilizer applications of solid union biology of the invention in rice, corn, wheat, herbage, lawn or
Fruits and vegetables are applied with base fertilizer, ditch spread or cave are applied, and play water conservation moisturizing, anti-continuous cropping, dissolving phosphor and dissolving potassium, strong plantlets and rootage, activating soil or suppression
The effect of sick insect prevention.
The more control water conservation moisturizing fertilizer of solid union biology of the invention, which can play, inhibits Rhizoctonia solani Kuhn, Pyricularia oryzae
With the effect of fusarium graminearum or wheat powdery mildew.
Biological more control water conservation moisturizing fertilizer increasingly have been favored by people as environmental friend fertilizer, its efficient, low toxicity,
It is low-residual, low cost, environmentally friendly, a large number of nutrients of crop needs is not only directly fed, but also by largely living
Microorganism it is actively movable, stimulate plant growth to provide the nutriment of crop needs or generate hormone, while there is life
Object pesticide, bio-feritlizer, biological nutrition and water conservation moisturizing quadruple effect.Biological more control water conservation moisturizing fertilizer contain a large amount of beneficial to micro-
Biology can be improved chemical fertilizer utilization ratio, reduce environmental pollution；Its organic matter being rich in can improve soil physical property, increase
Soil aggregate structure reduces soil hardening to make loosing soil, is conducive to water conservation, fertilizer conservation, ventilation and promotes root system development,
Comfortable growing environment is provided for crops；It is rich in organic matter and humus, and the openness of crop stomata, these objects are adjusted
Matter and beneficial microorganism metabolite (enzyme) act synergistically, and can be improved the synthesis resist ability of crop, to improve crop
Drought-resistant ability.The enhancing of soil aggregate structure promotes the permeability of capillary, ensure that the operating of soil moisture, improves
The utilization rate of soil moisture advocates production safety, nuisanceless green so that meeting China develops green agriculture, the ecological agriculture
The requirement of food.
In addition, annual grain processing plant is while producing a large amount of rice, a large amount of rice husk becomes waste or portion
It is allocated as feed addictive, or is burned off, but rice husk is keeping moisture, retentively mildly play compared with your writing in terms of improvement soil
With.Meanwhile sheep dung is very good organic fertilizer, moreover, in water conservation moisturizing, holding ground temperature, reduces a huge sum of money in soil
All it is worth the application of deeper degree in the addition of category.
The effect for each strain that the more control water conservation moisturizing fertilizer of solid union biology of the invention use is as follows:
Streptomyces hygroscopicus can generate antifungal antibiotic, inhibit Rhizoctonia solani Kuhn, Pyricularia oryzae and fusarium graminearum,
Wheat powdery mildew；
Bacillus megaterium is a kind of bacterial manure, can convert n-p-k element in soil, increase soil fertility, and irritating work
Object growth；
Bacillus amyloliquefaciens can be improved crop and endanger germ and adverse circumstance the Scavenging activity for generating oxygen in primosome, adjust
Cell microorganism environment maintains the normal physiological metabolism of cell and biochemical reaction, improves the resistance of crop, increase the guarantor of crop
Strong effect, can promote plant growth, improve yield；
Bacillus sphaericus is a kind of bacterium that can be generated to pest with single-minded insecticidal activity albumen, especially prevention and treatment soil
Nematode；
Bacillusmusilaginosiengineering is a kind of B. mucilaginocus, its effect is by the mineral K in soil, and being dissolved into can be by plant
The potassium directly absorbed；
Blown-ball Azotobacter is a kind of bacterial fertilizer, can secrete auxin, it can be straight by the nitrogen in air in process of production
Switch through and turn to the absorbent ammonium nitrogen of crops, nitrate nitrogen and organic nitrogen, to produce fertilizer efficiency；
Bacillus cereus can be improved crop and endanger germ and adverse circumstance the Scavenging activity for generating oxygen in primosome, adjust thin
Born of the same parents' microbial environment maintains the normal physiological metabolism of cell and biochemical reaction, improves the resistance of crop, increase the health care of crop
Effect can promote plant growth, improve yield；
Withered grass element, the gramicidins isoreactivity substance generated during bacillus subtilis thalli growth, there is pathogenic bacteria
Apparent inhibiting effect；
Thin Huang strepto- is a kind of bacterial manure, can convert nitrogen P elements in soil, increase soil fertility, and irritating plant growth
Effect；
Golden streptomyces chromogenes can generate antifungal antibiotic, inhibit Rhizoctonia solani Kuhn and Pyricularia oryzae；
Trichoderma viride, which generates chitinase and β -1,3- dextranase, to be considered playing weight in anti-plant pathogenic fungi
It acts on；Trichoderma viride is in the culture medium for being original carbon source with D- xylose, xylan or natural plant cells wall prepared product
Zytase can be generated, plant has apparent defense reaction under zytase effect；Trichoderma viride is in metabolic process
In can produce Antagonism chemical substance and carry out poisonous plant disease fungus, these substances include antibiotic and some enzymes.
The various bacterium solutions by suitable proportion mixture, can be kept each Microflora in composite bacteria agent by the present invention well
Ratio is relatively stable；Composite bacteria agent and the microbial flora decomposed by natural environment and with it is mixed without individually cultivating
Flora compare, using the flora of mixture together again after independent culture, since reasonable can be selected according to actual needs
With ratio, it can fully play the biological nature of each flora itself in microbial inoculum and mutually act synergistically, so carrying out biology with it
The scope of application of processing is very extensive.
The more control water conservation moisturizing fertilizer of solid union prepared by the present invention biology, containing total living bacteria count up to 1.0 hundred million/milliliter with
Upper (standard GB/T -20287-2006).
It is a kind of containing abundant and high value active microorganism bacterium that the present invention controls biological water-keeping moisturizing fertilizer using solid-state more
Bio-feritlizer, there is fixed nitrogen, phosphorus decomposing, Potassium release, absorb water tariff collection soil moisture, promote decomposition of soil organic matter, is antibacterial anti-
Worm improves Soil Microorganism group, improves the function of soil texture；The product be for agriculture forest and husbandry production actual demand and
It specially researches and develops, has the function of unique " absorption, storage, release, recycling ", moisturizing factor therein can adsorb in air
Steam, have nature Water-saving effect.It is free from environmental pollution, no phytotoxicity, noresidue, to safety of human and livestock.
A kind of solid-state of the invention controls biological water-keeping moisturizing fertilizer more, through field is used to apply with base fertilizer, ditch spread or cave are applied
It is as follows to obtain positive effect for comparative test:
1, containing abundant organic matter, soil texture can be improved, vegetable soil promotes soil to build up fertility；
2, with etc. the other fertilizer ratios of nutrients, crop yield 20%；
3, coordinate crops and absorb nutrition, stimulation and regulation plant growth, mitigate or reduce crop disease and insect, improve crop
Quality；
4, can by rainwater, snow-broth, irrigation water, air steam and various Nutrient Absorptions store slow release 2-
3 months, form a miniature reservoir around crop root, reduce leakage and be lost, and reduce irrigation times, water-saving 50% with
On；
5, microbial bacterial agent provides the microorganism beneficial to plant growth, the effects of by decomposing, converting, has very in soil
A variety of nutrients can just be absorbed by crops utilization；
6, soil fertility is repaired and protected, soil is allowed to have " reserve strength "；Contain the potassium being largely cured, P elements in soil and arrests
Dissolubility, indissoluble phosphorus cannot be activated by crop utilization by the activity of microorganism come to reduce the current year of phosphorus, potassium element
Input amount；
7, the anti-phytotoxicity of crop, fertilizer damage, low-temperature resistance are improved, the ability of fighting drought and hot dry wind prevents from lodging, prevents early ageing；
8, effectively prevent root rot, bacterial wilt, epidemic disease, wilt disease, verticillium wilt, base rot disease, blight dis-ease etc., effectively inhibit
The breeding of root-knot nematode；
9, it will cannot be discharged by multiple elements such as calcium, magnesium, boron, manganese, zinc, molybdenum, iron that crop is directly absorbed and utilized in soil
For crop absorption utilization out mitigates the nutritional deficiency disease and other physiological diseases of crop, while forming dominant microflora in root, resists
Soil-borne disease bacterium is infected, and crop is induced to generate multiple resistance albumen, improves crop disease-resistant, lodging tolerance, anti-continuous cropping effect
It is more obvious.
Specific embodiment
Following embodiment is only used for that the present invention is explained, and is not construed as limiting the scope of the claims restriction,
Other alternative means that those skilled in the art are contemplated that according to description, should all be in the protection of the claims in the present invention
Within the scope of.
The bacterium numbering for the trichoderma viride (Trichoderma viride Persoon ex Fries) that the present invention uses
For CGMCC3.2942, the bacterium numbering of bacillus cereus (Bacillus cereus) is CGMCC 1.895, huge gemma bar
The bacterium numbering of bacterium (Bacillus megaterium de Bary) is CGMCC1.1870, bacillus amyloliquefaciens
The bacterium numbering of (Bacillus amyloliquefaciens Priest et al.) is CGMCC1.1226, spherical gemma bar
The bacterium numbering of bacterium (Bacillus sphaericus Meyer et Neide) is CGMCC1.929, bacillusmusilaginosiengineering
The bacterium numbering of (Bacillus mucilaginosus Krasil ' nikov) is CGMCC1.910, blown-ball Azotobacter
The bacterium numbering of (Azotobacter chroococcum Beijerinck) is CGMCC1.212, bacillus subtilis
The bacterium numbering of (Bacillus subtilis Cohn) is CGMCC1.936, streptomyces hygroscopicus (stretomyces
Hygroscopicus bacterium numbering) is CGMCC4.940, golden streptomyces chromogenes (Streptomyces
Aureochromogenes bacterium numbering) is CGMCC4.909, streptomyces microflavus (Streptomyces microflavus)
Bacterium numbering be CGMCC4.891.
The microorganism fungus kind that the embodiment of the present invention utilizes for the trichoderma viride of fermentative degradation and phosphorus decomposing, potassium decomposing, is consolidated
The microbial flora of nitrogen, disease resisting effect selects the strain catalogue of China General Microbiological culture presevation administrative center version in 2012
Disclosed in strain.
Blown-ball Azotobacter, bacillus megaterium, bacillus amyloliquefaciens, the spherical gemma bar prepared in the embodiment of the present invention
Bacterium, bacillus cereus, bacillus subtilis and bacillusmusilaginosiengineering bacterium solution containing total living bacteria count up to 2.0 hundred million/milliliter
(standard GB/T -20287-2006) above it is spare to can be used as production bacterium solution.
The equipment used is granulated in the embodiment of the present invention as granulating disc.
The present invention is using the composite microbiological fertilizer of Hong Kong Bao Nong Biotechnology Co., Ltd production, the big biology collection in Qingdao sea
The compound bacteria seed manure of the Enteromorpha bio-bacterial manure of Co., Ltd, group production and Japanese silicate bacteria in conjunction with Taiwan promise Cattell actinomyces
Respectively as comparative example 1, comparative example 2 and comparative example 3.
The main experimental conditions of the embodiment of the present invention and comparative example, method and result are as follows:
Herbage test: in Inner Mongol Huolin Gol, 5 points of diagonal line samplings, every 1m2, actual measurement is carried out, herbage is calculated by point and produces
Amount, applies 25kg per acre；The area control group CK (for comparative example 1, comparative example 2 and comparative example 3)；
Lawn test: river, 5 points of diagonal line samplings, every 1m are assigned in Liaoyang2, actual measurement is carried out, turfgrass is calculated by point and produces
Amount, applies 25kg per acre；The area control group CK (for comparative example 1, comparative example 2 and comparative example 3)；
Corn trials: in the Xiuyan town Yang Jiabao experimental plot, 5 points of diagonal line samplings, 40 plants every are produced in terms of point, are applied per acre
25kg；The area control group CK (for comparative example 1, comparative example 2 and comparative example 3)；
Wheat test: in the experimental plot Changyi, Shandong Bu Zhuan, 5 points of diagonal line samplings, 40 plants every are produced in terms of point, are applied per acre
25kg；The area control group CK (for comparative example 1, comparative example 2 and comparative example 3)；
Capsicum, eggplant, tomato test: 5 points of diagonal line samplings, 6 plants every are produced in terms of point, apply 25kg per acre；Control group
The area CK (for comparative example 1, comparative example 2 and comparative example 3)；
Pear cv nanguo test: the stone bridge orchard on Anshan, the equidistant sampling of parallel lines, totally 5 points, 2 every are produced in terms of point, often
Hundred jin of fruits apply 5kg；The area control group CK (for comparative example 1, comparative example 2 and comparative example 3).
The embodiment of the present invention and comparative example are as shown in table 1 to the influence comparable situation of soil layer construction, organic matter, soil pH；
Table 1
Average product situation of the embodiment of the present invention compared with equal nutrients comparative example is as shown in table 2 and table 3；
Table 2
Table 3
The comparable situation of influence of the embodiment of the present invention with equal nutrients comparative example to cereal crops quality is as shown in table 4；
Table 4
The embodiment of the present invention and equal nutrients comparative example are as shown in table 5 to the qualitative effects comparable situation of fruit trees and vegetables；
Table 5
The products application of the embodiment of the present invention 2,5 or 8 is in paddy field, compared with using common fertilizer, make cultivation crab,
Loach, swamp eel net gain 8~12%.
The shelf life of products of the embodiment of the present invention is 12~18 months.
Culture medium that the bacterium solution of trichoderma viride uses is prepared in the embodiment of the present invention as PDA liquid medium, culture temperature
25 DEG C of degree, pH are 7.0~7.2, and the revolving speed that when culture stirs is 60~120 turns/min, the incubation time of each step is 48~
72 hours.
Blown-ball Azotobacter, bacillus megaterium, bacillus amyloliquefaciens, spherical gemma bar are prepared in the embodiment of the present invention
Bacterium, bacillus cereus, bacillus subtilis and bacillusmusilaginosiengineering the culture medium that uses of bacterium solution for beef extract-peptone
Fluid nutrient medium, 30 DEG C of cultivation temperature, pH is 7.2~7.8, and mixing speed is 60~120 turns/min when culture, each step
Incubation time is 20~48 hours.
The training that the bacterium solution of streptomyces hygroscopicus liquid, golden streptomyces chromogenes and streptomyces microflavus uses is prepared in the embodiment of the present invention
Supporting base is Gause I fluid nutrient medium, and 28 DEG C of cultivation temperature, pH is 7.0~7.2, and the revolving speed that when culture stirs is 60~120
Turn/min, the incubation time of each step 120~144 hours.
Embodiment 1
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 1 part of bentonite,
40 parts of matrix, 1 part of the bacterium solution of streptomyces hygroscopicus, 1 part of the bacterium solution of bacillus megaterium, 2 parts of the bacterium solution of bacillus amyloliquefaciens, ball
2 parts of the bacterium solution of shape bacillus, 7 parts of the bacterium solution of bacillusmusilaginosiengineering, 1 part of the bacterium solution of blown-ball Azotobacter, bacillus cereus
1 part of bacterium solution, 1 part of the bacterium solution of bacillus subtilis, 1 part of the bacterium solution of golden streptomyces chromogenes, 1 part of the bacterium solution of streptomyces microflavus is green
8 parts of the bacterium solution of color Trichoderma；
Matrix is powdered rice hulls；
Preparation method sequentially includes the following steps:
1, by streptomyces hygroscopicus, bacillus megaterium, bacillus amyloliquefaciens, Bacillus sphaericus, gel-shaped gemma bar
Bacterium, blown-ball Azotobacter, bacillus cereus, bacillus subtilis, golden streptomyces chromogenes, streptomyces microflavus and trichoderma viride are adopted
With three-level, bacterium solution is respectively prepared in fermentation method step by step, and preparation step includes slant strains activation culture, tapered shaking flask expansion culture, one
Grade fermentor liquid spreads cultivation and second order fermentation tank spreads cultivation four steps；The bacterium solution of acquisition streptomyces hygroscopicus, huge gemma bar respectively
The bacterium solution of bacterium, the bacterium solution of bacillus amyloliquefaciens, the bacterium solution of Bacillus sphaericus, the bacterium solution of bacillusmusilaginosiengineering, brown ball are solid
The bacterium solution of nitrogen bacterium, the bacterium solution of bacillus cereus, the bacterium solution of bacillus subtilis, the bacterium solution of golden streptomyces chromogenes, thin yellow strepto-
The bacterium solution of bacterium and the bacterium solution of trichoderma viride；
2, by the bacterium solution of streptomyces hygroscopicus, the bacterium solution of bacillus megaterium, the bacterium solution of bacillus amyloliquefaciens, spherical gemma
The bacterium solution of bacillus, the bacterium solution of bacillusmusilaginosiengineering, the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, withered grass gemma
The bacterium solution of bacillus, bacterium solution, the bacterium solution of streptomyces microflavus, the bacterium solution of trichoderma viride of golden streptomyces chromogenes mix with matrix
It is even, then with bentonite mixing granulation, then moisture removal is removed in drying under the conditions of 40~50 DEG C, and the more controls of solid union biology are made and protect
Water moisturizing fertilizer.
Embodiment 2
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 2 parts of bentonite,
40 parts of matrix, 2 parts of the bacterium solution of streptomyces hygroscopicus, 2 parts of the bacterium solution of bacillus megaterium, 3 parts of the bacterium solution of bacillus amyloliquefaciens, ball
3 parts of the bacterium solution of shape bacillus, 10 parts of the bacterium solution of bacillusmusilaginosiengineering, 2 parts of the bacterium solution of blown-ball Azotobacter, bacillus cereus
2 parts of bacterium solution, 2 parts of the bacterium solution of bacillus subtilis, 2 parts of the bacterium solution of golden streptomyces chromogenes, 2 parts of the bacterium solution of streptomyces microflavus is green
9 parts of the bacterium solution of color Trichoderma；
Matrix is straw powder；
The preparation method is the same as that of Example 1.
Embodiment 3
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 1 part of bentonite,
40 parts of matrix, 3 parts of the bacterium solution of streptomyces hygroscopicus, 3 parts of the bacterium solution of bacillus megaterium, 4 parts of the bacterium solution of bacillus amyloliquefaciens, ball
4 parts of the bacterium solution of shape bacillus, 15 parts of the bacterium solution of bacillusmusilaginosiengineering, 3 parts of the bacterium solution of blown-ball Azotobacter, bacillus cereus
3 parts of bacterium solution, 3 parts of the bacterium solution of bacillus subtilis, 3 parts of the bacterium solution of golden streptomyces chromogenes, 3 parts of the bacterium solution of streptomyces microflavus is green
10 parts of the bacterium solution of color Trichoderma；
Matrix is humic acid；
The preparation method is the same as that of Example 1.
Embodiment 4
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 1 part of bentonite,
40 parts of matrix, 1 part of the bacterium solution of streptomyces hygroscopicus, 1 part of the bacterium solution of bacillus megaterium, 2 parts of the bacterium solution of bacillus amyloliquefaciens, ball
2 parts of the bacterium solution of shape bacillus, 8 parts of the bacterium solution of bacillusmusilaginosiengineering, 1 part of the bacterium solution of blown-ball Azotobacter, bacillus cereus
1 part of bacterium solution, 1 part of the bacterium solution of bacillus subtilis, 1 part of the bacterium solution of golden streptomyces chromogenes, 1 part of the bacterium solution of streptomyces microflavus is green
8 parts of the bacterium solution of color Trichoderma；
Matrix is decomposed feces of livestock and poultry；
Decomposed feces of livestock and poultry selects the decomposed excrement of bovine；
The preparation method is the same as that of Example 1.
Embodiment 5
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: bentonite 10
Part, 90 parts of matrix, 3 parts of the bacterium solution of streptomyces hygroscopicus, 3 parts of the bacterium solution of bacillus megaterium, the bacterium solution 4 of bacillus amyloliquefaciens
Part, 4 parts of the bacterium solution of Bacillus sphaericus, 15 parts of the bacterium solution of bacillusmusilaginosiengineering, 3 parts of the bacterium solution of blown-ball Azotobacter, waxy bud
3 parts of the bacterium solution of spore bacillus, 3 parts of the bacterium solution of bacillus subtilis, 3 parts of the bacterium solution of golden streptomyces chromogenes, the bacterium solution 3 of streptomyces microflavus
Part, 10 parts of the bacterium solution of trichoderma viride；
Matrix is powdered rice hulls, humic acid, decomposed feces of livestock and poultry and straw powder, wherein 22.5 parts of powdered rice hulls, humic acid 22.5
Part, 22.5 parts of decomposed feces of livestock and poultry, 22.5 parts of straw powder；
Decomposed feces of livestock and poultry selects the decomposed excrement of horse species；
The preparation method is the same as that of Example 1.
Embodiment 6
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 8 parts of bentonite,
83 parts of matrix, 2 parts of the bacterium solution of streptomyces hygroscopicus, 2 parts of the bacterium solution of bacillus megaterium, 3 parts of the bacterium solution of bacillus amyloliquefaciens, ball
3 parts of the bacterium solution of shape bacillus, 12 parts of the bacterium solution of bacillusmusilaginosiengineering, 2 parts of the bacterium solution of blown-ball Azotobacter, bacillus cereus
2 parts of bacterium solution, 2 parts of the bacterium solution of bacillus subtilis, 2 parts of the bacterium solution of golden streptomyces chromogenes, 2 parts of the bacterium solution of streptomyces microflavus is green
9 parts of the bacterium solution of color Trichoderma；
Matrix is powdered rice hulls, humic acid and decomposed feces of livestock and poultry, wherein 10 parts of powdered rice hulls, 33 parts of humic acid, decomposed livestock and poultry
40 parts of excrement；
Decomposed feces of livestock and poultry selects the decomposed excrement of sheep class animal；
The preparation method is the same as that of Example 1.
Embodiment 7
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: bentonite 10
Part, 55 parts of matrix, 3 parts of the bacterium solution of streptomyces hygroscopicus, 3 parts of the bacterium solution of bacillus megaterium, the bacterium solution 4 of bacillus amyloliquefaciens
Part, 4 parts of the bacterium solution of Bacillus sphaericus, 15 parts of the bacterium solution of bacillusmusilaginosiengineering, 3 parts of the bacterium solution of blown-ball Azotobacter, waxy bud
3 parts of the bacterium solution of spore bacillus, 3 parts of the bacterium solution of bacillus subtilis, 3 parts of the bacterium solution of golden streptomyces chromogenes, the bacterium solution 3 of streptomyces microflavus
Part, 10 parts of the bacterium solution of trichoderma viride；
Matrix is one or more of powdered rice hulls, humic acid and straw powder, wherein 20 parts of powdered rice hulls, 10 parts of humic acid,
25 parts of straw powder；
The preparation method is the same as that of Example 1.
Embodiment 8
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 9 parts of bentonite,
68 parts of matrix, 3 parts of the bacterium solution of streptomyces hygroscopicus, 3 parts of the bacterium solution of bacillus megaterium, 3 parts of the bacterium solution of bacillus amyloliquefaciens, ball
3 parts of the bacterium solution of shape bacillus, 7 parts of the bacterium solution of bacillusmusilaginosiengineering, 1 part of the bacterium solution of blown-ball Azotobacter, bacillus cereus
1 part of bacterium solution, 1 part of the bacterium solution of bacillus subtilis, 1 part of the bacterium solution of golden streptomyces chromogenes, 1 part of the bacterium solution of streptomyces microflavus is green
8 parts of the bacterium solution of color Trichoderma；
Matrix is one or more of powdered rice hulls, decomposed feces of livestock and poultry and straw powder, wherein 35 parts of powdered rice hulls, decomposed poultry
10 parts of poultry manure, 23 parts of straw powder；
Decomposed feces of livestock and poultry selects the decomposed excrement of bovine；
The preparation method is the same as that of Example 1.
Embodiment 9
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 6 parts of bentonite,
66 parts of matrix, 1 part of the bacterium solution of streptomyces hygroscopicus, 1 part of the bacterium solution of bacillus megaterium, 2 parts of the bacterium solution of bacillus amyloliquefaciens, ball
2 parts of the bacterium solution of shape bacillus, 15 parts of the bacterium solution of bacillusmusilaginosiengineering, 3 parts of the bacterium solution of blown-ball Azotobacter, bacillus cereus
3 parts of bacterium solution, 3 parts of the bacterium solution of bacillus subtilis, 3 parts of the bacterium solution of golden streptomyces chromogenes, 3 parts of the bacterium solution of streptomyces microflavus is green
10 parts of the bacterium solution of color Trichoderma；
Matrix is one or more of humic acid, decomposed feces of livestock and poultry and straw powder, wherein 20 parts of humic acid, decomposed poultry
36 parts of poultry manure, 10 parts of straw powder；
Decomposed feces of livestock and poultry selects the decomposed excrement of horse species；
The preparation method is the same as that of Example 1.
Embodiment 10
The more control water conservation moisturizing fertilizer of solid union biology are made of the raw material components of following weight: 3 parts of bentonite,
50 parts of matrix, 1 part of the bacterium solution of streptomyces hygroscopicus, 3 parts of the bacterium solution of bacillus megaterium, 2 parts of the bacterium solution of bacillus amyloliquefaciens, ball
4 parts of the bacterium solution of shape bacillus, 7 parts of the bacterium solution of bacillusmusilaginosiengineering, 3 parts of the bacterium solution of blown-ball Azotobacter, bacillus cereus
1 part of bacterium solution, 3 parts of the bacterium solution of bacillus subtilis, 1 part of the bacterium solution of golden streptomyces chromogenes, 3 parts of the bacterium solution of streptomyces microflavus is green
9 parts of the bacterium solution of color Trichoderma；
Matrix is wherein 25 parts of powdered rice hulls in powdered rice hulls and straw powder, 25 parts of straw powder；
The preparation method is the same as that of Example 1.
Claims (1)
1. a kind of preparation method of the more control water conservation moisturizing fertilizer of solid union biology, it is characterised in that the solid union biology
More control water conservation moisturizing fertilizer are made of the raw material components of following weight: 1~10 part of bentonite, 40~90 parts of matrix, being inhaled
1~3 part of the bacterium solution of water streptomycete, 1~3 part of the bacterium solution of bacillus megaterium, 2~4 parts of the bacterium solution of bacillus amyloliquefaciens are spherical
2~4 parts of the bacterium solution of bacillus, 7~15 parts of the bacterium solution of bacillusmusilaginosiengineering, 1~3 part of the bacterium solution of blown-ball Azotobacter are waxy
1~3 part of the bacterium solution of bacillus, 1~3 part of the bacterium solution of bacillus subtilis, 1~3 part of the bacterium solution of golden streptomyces chromogenes, thin Huang chain
1~3 part of the bacterium solution of mould, 8~10 parts of the bacterium solution of trichoderma viride；The matrix is powdered rice hulls, humic acid, decomposed animal dung
Just one or more of with straw powder, wherein 10~40 parts of powdered rice hulls, 10~40 parts of humic acid, decomposed feces of livestock and poultry 10~40
Part, 10~40 parts of straw powder；The decomposed feces of livestock and poultry selects the decomposed excrement of ox, horse or sheep class animal；
Preparation method sequentially includes the following steps:
(1) bacterium solution is prepared: by streptomyces hygroscopicus, bacillus megaterium, bacillus amyloliquefaciens, Bacillus sphaericus, gel-shaped
Bacillus, blown-ball Azotobacter, bacillus cereus, bacillus subtilis, golden streptomyces chromogenes, streptomyces microflavus and green wood
Using three-level, bacterium solution is respectively prepared in fermentation method to mould step by step, and preparation step includes slant strains activation culture, the expansion of tapered shaking flask
Culture, one grade fermemtation tank liquid spread cultivation and second order fermentation tank spreads cultivation four steps；The bacterium solution, huge of streptomyces hygroscopicus is obtained respectively
The bacterium solution of bacillus, the bacterium solution of bacillus amyloliquefaciens, the bacterium solution of Bacillus sphaericus, the bacterium solution of bacillusmusilaginosiengineering,
It is the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, the bacterium solution of bacillus subtilis, the bacterium solution of golden streptomyces chromogenes, thin
The bacterium solution of yellow streptomycete and the bacterium solution of trichoderma viride；The culture medium that the bacterium solution of trichoderma viride uses is prepared as the training of PDA liquid
Base is supported, 25 DEG C of cultivation temperature, pH is 7.0~7.2, and the revolving speed that when culture stirs is 60~120 turns/min, the culture of each step
Time is 48~72 hours；Prepare blown-ball Azotobacter, bacillus megaterium, bacillus amyloliquefaciens, Bacillus sphaericus, waxy
The culture medium that the bacterium solution of bacillus, bacillus subtilis and bacillusmusilaginosiengineering uses is beef extract-peptone Liquid Culture
Base, 30 DEG C of cultivation temperature, pH is 7.2~7.8, and the revolving speed that when culture stirs is 60~120 turns/min, when the culture of each step
Between be 20~48 hours；The culture medium that the bacterium solution of preparation streptomyces hygroscopicus liquid, golden streptomyces chromogenes and streptomyces microflavus uses for
Gause I fluid nutrient medium, 28 DEG C of cultivation temperature, pH is 7.0~7.2, and the revolving speed that when culture stirs is 60~120 turns/min,
The incubation time of each step 120~144 hours；
(2) by the bacterium solution of streptomyces hygroscopicus, the bacterium solution of bacillus megaterium, the bacterium solution of bacillus amyloliquefaciens, spherical gemma bar
Bacterium solution, the bacterium solution of bacillusmusilaginosiengineering, the bacterium solution of blown-ball Azotobacter, the bacterium solution of bacillus cereus, bacillus subtilis of bacterium
The bacterium solution of bacterium, bacterium solution, the bacterium solution of streptomyces microflavus, the bacterium solution of trichoderma viride of golden streptomyces chromogenes are uniformly mixed with matrix,
Again with bentonite mixing granulation, then moisture removal is removed in drying under the conditions of 40~50 DEG C, and the more control water conservations of solid union biology are made
Moisturizing fertilizer.
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