CN110194708A - A kind of ferment slag soil remediation microbial inoculum and the preparation method and application thereof - Google Patents
A kind of ferment slag soil remediation microbial inoculum and the preparation method and application thereof Download PDFInfo
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- CN110194708A CN110194708A CN201910606183.4A CN201910606183A CN110194708A CN 110194708 A CN110194708 A CN 110194708A CN 201910606183 A CN201910606183 A CN 201910606183A CN 110194708 A CN110194708 A CN 110194708A
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- microbial inoculum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of preparation method and application of ferment slag soil remediation microbial inoculum, and including the following steps: for the ferment slag, clay class mineral carrier to be sieved respectively is made 60-80 mesh powder；The fermented liquid by functional bacteria fermented and above-mentioned powder and glucide ratio by weight are mixed again, wherein function bacterium viable count is hundred million cfu/g of 30-50；Wherein, ferment slag is as one of carrier, nutriment needed for nutritional ingredient therein can provide growth metabolism for function bacterium kind, micro-ecological environment in soil is improved simultaneously, it can be increased the quantity of Soil Microorganism, and beneficial bacterium thallus or its metabolite can directly or indirectly enhance soil fertility, promote plant growth, to improve crop yield and quality；Clay class carrier can synergistic function microorganism reduction heavy metal in soil biological effectiveness.
Description
Technical field
The present invention relates to contaminated soil administer field, in particular to the preparation method of a kind of ferment slag soil remediation microbial inoculum and
Using.
Background technique
With the development of industrial and agricultural production, heavy metal pollution of soil is further serious, and soil pollution, which has become, to receive much attention
One of public hazards.Cumulative for many years, the land reclamation that people face and ecological recovery problem are extremely serious.Biological renovation method
It is concerned because of its environmental protection, the advantages that without secondary pollution.
Chinese patent CN 108650918A discloses a kind of method using composite enzyme improvement salinization of soil farmland, changes
It is good to work well；Chinese patent CN 107309260A discloses a kind of application of environmental protection ferment in terms of heavy metal pollution reparation,
This method carries out soil remediation as acid eluent after diluting in proportion by preparing acid enzyme stoste；Above method body
The mistake for having showed application of the ferment in terms of soil remediation, having illustrated that it has a good application prospect, but produced or prepare in ferment
Cheng Zhong, ferment slag is typically no to be utilized well.
Summary of the invention
In order to solve the problems, such as background technique kind, ferment slag soil remediation microbial inoculum and its system are utilized the present invention provides a kind of
Preparation Method and application, ferment slag can provide needed for growth metabolism as one of carrier, nutritional ingredient therein for function bacterium kind
Nutriment, promote microorganism to the adsorption effect of Heavy Metals in Contaminated Soils.
The present invention provides a kind of ferment slag soil remediation microbial inoculum, raw material components include: ferment slag by ratio of weight and the number of copies
20-25 parts, 10-20 parts of clay class mineral carrier, 3-9 parts of glucide, 10-15 parts of function bacterium bacterium powder；The function bacterium bacterium powder
Living bacteria count be hundred million cfu/g of 30-50.
Wherein, the ferment slag is Noni fruit ferment slag, sea-buckthorn slag, aloe ferment slag, Melaleuca alternifolia ferment
One of slag, lemon ferment slag, pomelo peel ferment slag, blueberry ferment slag and fructus lycii ferment slag are a variety of.
Wherein, the clay class mineral carrier is illite, montmorillonite, needle ironstone, medical stone, rectorite, bentonite, sea
One or more of afrodite, kaolin and vermiculite.
Wherein, the glucide is one of soluble starch, molasses, glucose, sucrose, fructose and maltodextrin
Or it is several.
Wherein, the function bacterium is Delftiatsuruhatensis, Achromobacter xylosoxidans, moves Sphingol single-cell, huge bud less
One of born of the same parents bacillus, bending bacillus, soil coccus, Bacillus sphaericus and Bacillus subtillis are a variety of.
The present invention also provides the preparation methods of the ferment slag soil remediation microbial inoculum described in one kind, comprising steps of
(1) the ferment slag, clay class mineral carrier are sieved and 60-80 mesh powder is made；
(2) mixing: by the end of ferment ground-slag obtained in function bacterium bacterium powder, step (1), clay class mineral carrier powder and sugar
Substance is uniformly mixed, and obtains the ferment slag soil remediation microbial inoculum.
Wherein, the function bacterium bacterium powder the preparation method comprises the following steps:
(1) inclined-plane culture: function bacteria strain is aseptically inoculated on slant medium, is cultivated at 28-32 DEG C
16-24h obtains slant strains；
(2) prepared by seed liquor: the slant strains that step (1) is cultivated aseptically are inoculated in seed culture medium,
28-32 DEG C, cultivate 14-18h under the conditions of 140-200r/min；
(3) it ferments: the cultured function bacterium seed liquor of step (2) is aseptically incubated to the liquid fermentation of sterilizing
In culture medium, 0.05~0.06MPa, 28-32 DEG C, the condition that ventilation quantity is 1:0.4~0.6vvm are pressed in pH 6.5-7.2, tank
Under, 48-56h is cultivated to get fermented liquid by functional bacteria.
(4) it is spray-dried: gained fermented liquid by functional bacteria in step (3) being spray-dried, function bacterium bacterium powder is obtained.
Wherein, the liquid fermentation medium in the step (3) includes carbon source, nitrogen source and organic salt, the carbon source are as follows: bran
One or more of Pi Fen, bean cake powder, soybean powder, groundnut meal, glucose, sucrose, fructose, brown sugar, soluble starch, institute
State nitrogen source be one or more of yeast powder, peptone, fish meal, dried silkworm chrysalis meal, corn pulp, ammonium sulfate, ammonium nitrate, ammonium chloride,
The inorganic salts are sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium chloride, potassium nitrate, sodium nitrate, ferrous sulfate
One or more of.
The deposit number of the function bacterium Delftiatsuruhatensis is the deposit number of ACCC 01145, Achromobacter xylosoxidans
For ACCC 01146, the deposit number of dynamic Sphingol single-cell is ACCC 01119, the deposit number of Bacillus megatherium is less
ACCC 01030, be bent bacillus deposit number be ACCC01069, the deposit number of soil coccus be ACCC 01035,
The deposit number of Bacillus sphaericus is ACCC 01116, the deposit number of Bacillus subtillis is ACCC 19373.
The present invention also provides a kind of application of ferment slag soil remediation microbial inoculum on farmland or arable land.
Wherein, the ferment slag soil remediation microbial inoculum single administration amount is 22-32kg/ mus；It is preferred that the ferment slag soil
Remediation microbial inoculum single administration amount is 28kg/ mus.
Above scheme of the invention have it is following the utility model has the advantages that
(1) present invention provides a kind of ferment slag soil remediation microbial inoculum, ferment slag as one of carrier, nutrition therein at
Divide nutriment needed for can providing growth metabolism for function bacterium kind, while improving micro-ecological environment in soil, Ji Kezeng
Add the quantity of Soil Microorganism, and beneficial bacterium thallus or its metabolite can directly or indirectly enhance soil fertility, promotes
Paddy growth, to improve crop yield and quality；
(2) clay class mineral carrier have good heavy metal adsorption characteristic, can synergistic function microorganism reduce soil in
Bioavailability of heavy metals, wherein microorganism can pass through the adsorption effect of thallus and its metabolite in reproductive process
To the heavy metal in the chelation fixing soil of heavy metal；In addition, mostly inorganic containing potassium, magnesium, phosphorus etc. in clay class mineralogical composition
Element, it is further provided nutritional ingredient needed for plant growth.
Specific embodiment
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with specific implementation
Example is described in detail.
Embodiment 1
A kind of ferment slag soil remediation microbial inoculum, in parts by weight, raw material components include: Noni fruit ferment slag and sea-buckthorn
22.5 parts of the mixture of ferment slag, illite, 6 parts of 15 parts of mixture, the molasses of montmorillonite, soil coccus bacterium powder and Ko subtilis
13 parts of bacillus bacterium powder mixture；The living bacteria count of the soil coccus bacterium powder is 2,000,000,000 cfu/g, the bacillus subtilis bacterium
The living bacteria count of powder is 2,500,000,000 cfu/g.
The preparation method of above-mentioned ferment slag soil remediation microbial inoculum, comprising steps of
(1) the ferment slag and illite, montmorillonite mixture are sieved respectively and 60-80 mesh powder is made；
(2) mixing: by the end of ferment ground-slag obtained in function bacterium bacterium powder, step (1), clay class mineral carrier powder and sugar
Substance is uniformly mixed, and obtains the ferment slag soil remediation microbial inoculum.
Wherein, the soil coccus and Bacillus subtillis bacterium powder the preparation method comprises the following steps:
(1) inclined-plane culture: soil meningitidis strains and Bacillus subtillis bacterial strain are aseptically inoculated in tiltedly respectively
On the culture medium of face, 16-24h is cultivated under the conditions of 28-32 DEG C；
(2) prepared by seed liquor: the slant strains of step (1) culture are aseptically inoculated in the ratio of 10-15%
In seed culture medium, 28-32 DEG C, cultivate 14-18h under the conditions of 140-200r/min；
(3) it ferments: aseptically respectively or mixed culture is in going out by the cultured function bacterium seed liquor of step (2)
In the liquid fermentation medium of bacterium, pH 6.5-7.2, tank press 0.05~0.06MPa, 28-32 DEG C, ventilation quantity 1:0.4~
Under conditions of 0.6vvm, 48-56h is cultivated.
(4) it is spray-dried: gained fermented liquid by functional bacteria in step (3) being spray-dried, soil coccus bacterium powder is obtained
With bacillus subtilis bacterium powder.
The inclined-plane culture based formulas (g/L) are as follows: peptone 10, beef extract 10, sodium chloride 5.0, pH7.0-7.2；
The seed culture based formulas (g/L) are as follows: glucose sugar 20, peptone 20, yeast extract 10, pH 7.0-7.2；
The liquid fermentation medium formula (g/L) are as follows: wheatfeed 5.0, glucose 5.0, peptone 2.0, fish meal 3.0,
Sodium chloride 1.0, magnesium sulfate 2.0, potassium dihydrogen phosphate 0.5, ferrous sulfate 0.01, pH 7.0-7.2.
Embodiment 2
A kind of preparation method of ferment slag soil remediation microbial inoculum, raw material components are by ratio of weight and the number of copies are as follows: Noni fruit ferment
Slag and 24 parts of sea-buckthorn slag mixture, illite, 12 parts of montmorillonite mixture, 8 parts of molasses, soil coccus and Ko subtilis bar
11 parts of bacterium bacterium powder；The living bacteria count of the soil coccus is 3,000,000,000 cfu/g, and the living bacteria count of the bacillus subtilis is
2000000000 cfu/g.
The preparation method is the same as that of Example 1 for subsequent soil coccus and bacillus subtilis powder.
Embodiment 3
A kind of preparation method of ferment slag soil remediation microbial inoculum, raw material components are by ratio of weight and the number of copies are as follows: Noni fruit ferment
Slag and 21 parts of sea-buckthorn slag mixture, illite, 10 parts of montmorillonite mixture, 4 parts of molasses, soil coccus and Ko subtilis bar
15 parts of bacterium bacterium powder；Wherein 1,500,000,000 cfu/g of living bacteria count position of the soil ball bacterium, effective viable bacteria of the bacillus subtilis
Number is 1,800,000,000 cfu/g.
The preparation method is the same as that of Example 1 for subsequent soil coccus and bacillus subtilis powder.
Test example 4
This test example further provides a kind of application of ferment slag soil remediation microbial inoculum on farmland or arable land, predominantly
Cadmium pollution soil.
Test site: Liuyang City, Hunan Province Yongan town
Test period: July in April-
Experimental design:
9 pieces of rice fields similar in size are chosen, and water system is independent of each other, each test group is provided that
Control group 1: pollution-free rice field, non-treated measure；
Control group 2: pollution rice field, non-treated measure；
Control group 3: Noni fruit ferment slag is not added in formula by bacterial preparation process in above-described embodiment 1 in pollution rice field
With sea-buckthorn slag, 10-15 days 22kg/ mus of application soil remediation microbial inoculum before Rice Cropping or rice transplanting；
Control group 4: molasses are not added in formula, in rice by bacterial preparation process in above-described embodiment 1 in pollution rice field
10-15 days 22kg/ mus of slag soil remediation microbial inoculum of application ferment before plantation or rice transplanting；
Control group 5: multi-functional bacterium bacterium is not added in formula by bacterial preparation process in above-described embodiment 1 in pollution rice field
Powder, 10-15 days 22kg/ mus of slag soil remediation microbial inoculum of application ferment before Rice Cropping or rice transplanting；
Control group 6: pollution rice field does not add mineral substance carrier in formula by bacterial preparation process in above-described embodiment 1,
10-15 days 22kg/ mus of slag soil remediation microbial inoculum of application ferment before Rice Cropping or rice transplanting；
Experimental group 1: pollution rice field, the ferment slag soil in 10-15 days application above-described embodiments 1 before Rice Cropping or rice transplanting
22kg/ mus of remediation microbial inoculum；
Experimental group 2: pollution rice field, the ferment slag soil in 10-15 days application above-described embodiments 1 before Rice Cropping or rice transplanting
25kg/ mus of remediation microbial inoculum；
Experimental group 3: pollution rice field, the ferment slag soil in 10-15 days application above-described embodiments 1 before Rice Cropping or rice transplanting
28kg/ mus of remediation microbial inoculum；
Experimental group 4: pollution rice field, the ferment slag soil in 10-15 days application above-described embodiments 1 before Rice Cropping or rice transplanting
32kg/ mus of remediation microbial inoculum.
When specific operation, each test group sowing or rice transplanting, fertilising, watering, weeding, the prevention and control of plant diseases, pest control are completed in 1 day,
Human error is reduced to minimum；Fertilising is carried out by local Fertilization Level and fertilizing method, according to weather and soil moisture
Situation opens up irrigation canals and ditches, carries out prevention waterlogging drought resisting and prepares.
The ferment slag soil remediation microbial inoculum is applied using artificial, and after rice harves, measurement rice yield, the effective cadmium of soil contain
Amount, rice cadmium content；1 is shown in Table for the examination main agrochemical characteristic of soil.
The physicochemical character of 1 soil of table
Test result:
Table 2 is that resulting ferment slag soil remediation microbial inoculum is prepared according to method described in embodiment 1 to soil and rice
Improved effect.
Experimental result as shown in Table 2 is it is found that ferment slag soil remediation microbial inoculum provided by the invention can be effectively reduced
The biological effectiveness of cadmium in soil harvests effectively cadmium content in the soil of front and back and significantly decreases, and the cadmium content in rice, phase
Than in control group 2, content reduces, while the per mu yield of rice effectively improves.
In addition, the experimental result of control group 3-6 is it is found that in the feelings for not adding ferment slag, glucide and function bacterium respectively
Prepared microbial inoculum under condition still has improved effect compared to control group 2；But it can not show a candle to according to method system described in embodiment 1
Standby resulting ferment slag soil remediation microbial inoculum to the improved effect of soil and rice, i.e. repair by the ferment slag soil in experimental group 1~4
Improved effect of the multiple microbial inoculum to soil and rice.
In the use process of ferment slag soil remediation microbial inoculum, the 22-32kg remediation microbial inoculum is added in farmland per acre is advisable；It is excellent
Selection of land adds the 28kg ferment slag soil remediation microbial inoculum per acre in farmland.
Above-mentioned experiment is carried out with method using the ferment slag soil remediation microbial inoculum of other embodiments preparation, is achieved and embodiment
2 or the similar function and effect of embodiment 3.
In addition, ferment slag, carrier employed in embodiment 1-3, glucide, function bacterium type are all the same, only match not
Together, and only listing a kind of combination, the present invention will be described, due to each substance combination multiplicity used by according to the present invention,
Therefore it can be combined according to actual needs.
Ferment slag soil remediation microbial inoculum provided by the invention, for ferment slag as one of carrier, nutritional ingredient therein can be with
Nutriment needed for providing growth metabolism for function strain, while micro-ecological environment in soil is improved, it can be increased soil
The quantity of middle microorganism, and beneficial bacterium thallus or its metabolite can directly or indirectly enhance soil fertility, promote crop raw
It is long, to improve crop yield and quality；Clay class carrier can synergistic function microorganism reduction heavy metal in soil biologically effective
Property.Ferment slag, clay class mineral and cadmium content in soil is effectively reduced beneficial to thallus synergistic effect, while ferment slag makes
During solving and referring to the production of existing ferment or preparation in background technique, ferment slag is typically no to be obtained well
The problem of utilization.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of ferment slag soil remediation microbial inoculum, which is characterized in that its raw material components includes: ferment slag 20- by ratio of weight and the number of copies
25 parts, 10-20 parts of clay class mineral carrier, 3-9 parts of glucide, 10-15 parts of function bacterium bacterium powder；The function bacterium bacterium powder has
Effect viable count is hundred million cfu/g of 30-50.
2. ferment slag soil remediation microbial inoculum according to claim 1, which is characterized in that the ferment slag is Noni fruit ferment
Slag, sea-buckthorn slag, aloe ferment slag, Melaleuca alternifolia ferment slag, lemon ferment slag, pomelo peel ferment slag, blueberry ferment
One of slag and fructus lycii ferment slag are a variety of.
3. ferment slag soil remediation microbial inoculum according to claim 1, which is characterized in that the clay class mineral carrier is Erie
One or more of stone, montmorillonite, needle ironstone, medical stone, rectorite, bentonite, sepiolite, kaolin and vermiculite.
4. ferment slag soil remediation microbial inoculum according to claim 1, which is characterized in that the glucide is soluble forms sediment
One or more of powder, molasses, glucose, sucrose, fructose and maltodextrin.
5. ferment slag soil remediation microbial inoculum according to claim 1, which is characterized in that the function bacterium be Delftiatsuruhatensis,
Achromobacter xylosoxidans move Sphingol single-cell, Bacillus megatherium, bending bacillus, soil coccus, spherical brood cell less
One of bacillus and Bacillus subtillis are a variety of.
6. a kind of preparation method of ferment slag soil remediation microbial inoculum according to any one of claims 1 to 5, which is characterized in that
Comprising steps of
(1) the ferment slag, clay class mineral carrier are sieved respectively and 60-80 mesh powder is made；
(2) mixing: by the end of ferment ground-slag obtained in function bacterium bacterium powder, step (1), clay class mineral carrier powder and carbohydrate object
Matter is uniformly mixed, and obtains the ferment slag soil remediation microbial inoculum.
7. the preparation method of ferment slag soil remediation microbial inoculum according to claim 6, which is characterized in that the function bacterium bacterium
Powder the preparation method comprises the following steps:
(1) inclined-plane culture: function bacteria strain is aseptically inoculated on slant medium, cultivates 16- at 28-32 DEG C
For 24 hours, slant strains are obtained；
(2) prepared by seed liquor: the slant strains that step (1) is cultivated aseptically being inoculated in seed culture medium, 28-32
DEG C, cultivate 14-18h under the conditions of 140-200r/min；
(3) it ferments: the cultured function bacterium seed liquor of step (2) is aseptically incubated to the liquid fermentation and culture of sterilizing
In base, pH 6.5-7.2, tank press 0.05~0.06MPa, 28-32 DEG C, ventilation quantity be 1:0.4~0.6vvm under conditions of, training
48-56h is supported to get fermented liquid by functional bacteria；
(4) it is spray-dried: gained fermented liquid by functional bacteria in step (3) being spray-dried, function bacterium bacterium powder is obtained.
8. the preparation method of ferment slag soil remediation microbial inoculum according to claim 7, which is characterized in that the step (3)
In liquid fermentation medium include carbon source, nitrogen source and organic salt, the carbon source are as follows: wheatfeed, bean cake powder, soybean powder, peanut
One or more of cake powder, glucose, sucrose, fructose, brown sugar, soluble starch, the nitrogen source be yeast powder, peptone,
One or more of fish meal, dried silkworm chrysalis meal, corn pulp, ammonium sulfate, ammonium nitrate, ammonium chloride, the inorganic salts are sodium chloride, sulfuric acid
One or more of magnesium, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium chloride, potassium nitrate, sodium nitrate, ferrous sulfate.
9. a kind of application of the ferment slag soil remediation microbial inoculum on farmland or arable land as described in any one of claim 1-5.
10. application according to claim 8, which is characterized in that the ferment slag soil remediation microbial inoculum single administration amount is
22-32kg/ mus；It is preferred that the ferment slag soil remediation microbial inoculum single administration amount is 28kg/ mus.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111116219A (en) * | 2019-12-16 | 2020-05-08 | 上海洁壤环保科技有限公司 | Preparation process and application method of recyclable soil remediation ball |
| CN115141769A (en) * | 2022-06-21 | 2022-10-04 | 湖南省蔬菜研究所 | Culture medium for improving thallus abundance of achromobacter or enterobacter and fermentation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105537264A (en) * | 2016-01-19 | 2016-05-04 | 济南益邦生物科技有限公司 | Bioremediation agent for treating compound contaminated soil and application thereof |
| CN105777329A (en) * | 2016-02-04 | 2016-07-20 | 鞍山禾瑞生物科技有限公司 | Solid composite biological multi-control water and moisture retention fertilizer and preparing method and application thereof |
| CN105820008A (en) * | 2016-03-29 | 2016-08-03 | 广东植物龙生物技术股份有限公司 | Soil restoration conditioner for heavy metal pollution and preparation method thereof |
| CN106187364A (en) * | 2016-07-02 | 2016-12-07 | 合肥市田然农业科技园有限公司 | A kind of landscape flower dedicated fertilizer |
| CN106220400A (en) * | 2016-07-07 | 2016-12-14 | 湖北益丰生物肥业有限公司 | A kind of microorganism acidity soil-repairing agent and its preparation method and application |
| CN106396931A (en) * | 2016-08-31 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof |
| CN107445742A (en) * | 2017-09-28 | 2017-12-08 | 惠州市支持网知识产权运营有限公司 | A kind of biological organic chemical fertilizer |
| CN107457252A (en) * | 2017-08-04 | 2017-12-12 | 蓝畅 | One heavy metal species and（Or）The restorative procedure of organic hydrocarbon contaminated soil |
| CN108456110A (en) * | 2018-04-04 | 2018-08-28 | 安徽省强家庄生态农业有限公司 | A kind of winter vegetable organic base fertilizer and preparation method thereof |
| CN109576181A (en) * | 2018-12-18 | 2019-04-05 | 湖南泰谷生态工程有限公司 | A kind of modified illite powder microbial bacterial agent and the preparation method and application thereof |
-
2019
- 2019-07-05 CN CN201910606183.4A patent/CN110194708A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105537264A (en) * | 2016-01-19 | 2016-05-04 | 济南益邦生物科技有限公司 | Bioremediation agent for treating compound contaminated soil and application thereof |
| CN105777329A (en) * | 2016-02-04 | 2016-07-20 | 鞍山禾瑞生物科技有限公司 | Solid composite biological multi-control water and moisture retention fertilizer and preparing method and application thereof |
| CN105820008A (en) * | 2016-03-29 | 2016-08-03 | 广东植物龙生物技术股份有限公司 | Soil restoration conditioner for heavy metal pollution and preparation method thereof |
| CN106187364A (en) * | 2016-07-02 | 2016-12-07 | 合肥市田然农业科技园有限公司 | A kind of landscape flower dedicated fertilizer |
| CN106220400A (en) * | 2016-07-07 | 2016-12-14 | 湖北益丰生物肥业有限公司 | A kind of microorganism acidity soil-repairing agent and its preparation method and application |
| CN106396931A (en) * | 2016-08-31 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof |
| CN107457252A (en) * | 2017-08-04 | 2017-12-12 | 蓝畅 | One heavy metal species and（Or）The restorative procedure of organic hydrocarbon contaminated soil |
| CN107445742A (en) * | 2017-09-28 | 2017-12-08 | 惠州市支持网知识产权运营有限公司 | A kind of biological organic chemical fertilizer |
| CN108456110A (en) * | 2018-04-04 | 2018-08-28 | 安徽省强家庄生态农业有限公司 | A kind of winter vegetable organic base fertilizer and preparation method thereof |
| CN109576181A (en) * | 2018-12-18 | 2019-04-05 | 湖南泰谷生态工程有限公司 | A kind of modified illite powder microbial bacterial agent and the preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 范丽等: "土壤粘土矿物与微生物交互关系及其治理重金属污染的应用浅析", 《矿物学报》 * |
| 荣兴民等: "土壤矿物与微生物相互作用的机理及其环境效应", 《生态学报》 * |
| 高利娟等: "微生物与土壤矿物相互作用的研究进展", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111116219A (en) * | 2019-12-16 | 2020-05-08 | 上海洁壤环保科技有限公司 | Preparation process and application method of recyclable soil remediation ball |
| CN115141769A (en) * | 2022-06-21 | 2022-10-04 | 湖南省蔬菜研究所 | Culture medium for improving thallus abundance of achromobacter or enterobacter and fermentation method thereof |
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