CN104496707B - Fertilizer and preparation method and application is controlled a kind of liquid compound bio more - Google Patents
Fertilizer and preparation method and application is controlled a kind of liquid compound bio more Download PDFInfo
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- CN104496707B CN104496707B CN201510013030.0A CN201510013030A CN104496707B CN 104496707 B CN104496707 B CN 104496707B CN 201510013030 A CN201510013030 A CN 201510013030A CN 104496707 B CN104496707 B CN 104496707B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The present invention relates to control fertilizer a kind of liquid compound bio more, it is characterised in that it is made up of the raw material of following weight：45~65 parts of 1~3 part of the brown strepto- bacterium solution of 1~3 part of streptomyces hygroscopicus liquid, Chinese cassia tree, 2~4 parts of bacillus amyloliquefaciens liquid, 2~4 parts of Bacillus sphaericus liquid, 7~15 parts of bacillusmusilaginosiengineering liquid, 1~3 part of blown-ball Azotobacter liquid, golden 1~3 part of streptomyces chromogenes liquid, 8~10 parts of the fermentative degradation liquid of trichoderma viride, 8~10 parts of matrix and sterilized water.It is prepared for the carbohydrate and organic matter trichoderma viride fermentative degradation liquid of crops absorption, efficiently beneficial phosphorus decomposing, potassium decomposing, fixed nitrogen, the microorganism species of disease resisting effect are provided, biological pesticide, bio-feritlizer and the effect of biological nutrition triple function can be played simultaneously, but it is nonhazardous, free from environmental pollution.With waiting the other fertilizer ratios of nutrient, crop yield 20%.
Description
Technical field
It is particularly a kind of to be used for paddy rice, corn, wheat and fruits and vegetables seed soaking, leaf the present invention relates to technical field of biological fertilizer
Face spray or the liquid compound bio of pouring root more than control fertilizer and preparation method and application.
Background technology
A large amount of with chemical fertilizer particularly nitrogenous fertilizer use, and are enriched more nitrate in grain, nutritional quality and
Mouthfeel has all declined, and can not meet the health diet consumption need that people pursue nutritious, tasty, nuisanceless meals
Ask.Long-term largely to reduce the increase of soil inorganic salt content, organic matter using chemical fertilizer, soil loses acid-base balance, soil property plate
Knot；And humic acid, moisture and nutrient famine in soil, cause soil desertification；Fertilizer be crop " grain ",
" nutrition ", is directly connected to the yield of crop and the quality of product.Now both at home and abroad all in actively development green agriculture, ecological agriculture
Industry, advocates production safety, pollution-free green food.During pollution-free food is produced, then require without or try one's best few useization
Learn fertilizer, chemical pesticide and other chemical substances.
Studied to solve the above problems, China has put into a large amount of manpower and materials.Composite microbic bacterial fertilizer, especially gives birth to
Controlling fertilizer thing as environmental friend fertilizer increasingly by people to be favored, it not only directly feeds a large amount of battalion of crop needs more
Material is supported, but also it is actively movable by microorganism largely living, to provide the nutriment of crop needs or produce hormone
Plant growth is stimulated, while having biological pesticide, bio-feritlizer and biological nutrition triple function.
At present, the research about biologic compound fertilizer or biological many control fertilizer has：Patent document CN1970506A, it is polynary
Composite microbic bacterial fertilizer；CN102826895A, a kind of multielement compound microbe bacterial fertilizer and preparation method thereof；CN103204732A,
Composite microbic bacterial fertilizer of the Producing Strain containing polyglutamic acid and preparation method and application；CN102910985A, a kind of organic composite
Microbial-bacterial fertilizer and preparation method thereof, microbial-bacterial fertilizer is by zymocyte liquid, turfy soil, Chicken dung and diammonium hydrogen phosphate group
Into；A kind of CN102584453A, composite microbic bacterial fertilizer and preparation method thereof by plant ash, fermentation medium and is constituted
Itself nitrogen-fixing bacteria, solution organophosphor bacterium conciliate Phos bacterium and constituted；CN102951966A, a kind of producer of composite microbic bacterial fertilizer
Method, it is to use quail excrement and vinasse through nitrogen-fixing bacteria, phosphate solubilizing bacteria, the microbial bacterial agent of potassium solubilizing bacteria and fermentation for primary raw material system
Standby；CN102910988A, a kind of complex microorganism liquid bacterial fertilizer of preventing and treating plant pest and preparation method thereof, it is by symbiosis
The preparations such as beneficial flora fermentation Chinese prickly ash, capsicum, ginkgo leaf, kuh-seng, garlic coexist；A kind of CN101851123A, high concentration is answered
Microbial-bacterial fertilizer and its production method are closed, by blown-ball Azotobacter, bacillusmusilaginosiengineering, bacillus megaterium, lichens gemma bar
Preparation is cultivated and fermented to bacterium, the microbial inoculum of Bacillus macerans respectively.
The composite microbiological fertilizer of in the market application is less, and still, application effect is fine.For example, Hong Kong treasured agriculture is biological
The composite microbiological fertilizer of Science and Technology Ltd.'s production, using ocean high activity Fish protein and marine alga as main organic matter material,
A variety of biocontrol microorganisms and function bacterium are added, while added with micronutrient member in Chelating state necessary to amino acid and plant growth
Element, with enhancing soil activation, suppresses and prevents the generation of soil-borne disease, strengthens crop resistance, and fixing nitrogen, dissolving phosphor and dissolving potassium is improved comprehensive
Plant recovery of nutrient is closed, improving quality improves effect of yield.The also Enteromorpha life of Qingdao Seawin Biotech Group Co., Ltd.'s production
Thing bacterial manure, containing 5 plants of beneficial bacteriums, into soil after can quickly generate dominant microflora, form the microecological balance system of soil health
System, strengthens the disease resistance of trees；Simultaneously rich in a variety of nutriments beneficial to arboreal growth, such as organic matter, sea grass polysaccharide, waterside
Tongue oligosaccharides, enteromorpha fiber and a variety of middle trace elements, the immunity of balanced nutritious enhancing trees body is provided for trees, is prevented
Later stage various soil-borne diseases, it is flourishing.For another example the NEW TYPE OF COMPOSITE bacterium that Japanese silicate bacteria is combined with Taiwan promise Cattell actinomyces
Kind, fungus treatment forms three-dimensional protective barrier around crop root, and the rapid growth and breeding for suppressing soil surface characters, protection is made
Thing root system, to the dead seedling caused by continuous cropping and soil-borne disease, dead, rotten and other effects protrusion, purification soil can effectively decompose work
Thing residue and residue decompose the soil poisons such as the organic acid of generation, so that the fast quick-recovery of crop is grown up strong and sturdy, continuously make
Continuous cropping hydroxyisoxazole is used, soil hardening, chesson, reduction soil alkaline can be eliminated and poisoned, while biological inoculum is to solid in soil
N, P, K and various trace elements of change quickly dissolve release, balanced soil nutrient, Rapid reversal deadlock seedling, weak seedling, seedling, yellow leaf
Etc. various nutritional deficiency phenomenons.
Nevertheless, many control fertilizer of the biology that effective combination of microbial-bacterial fertilizer and organic fertilizer can be played, undoubtedly into
It is also the developing direction of following fertilizer application to solve the optimal collocation of problem now.Therefore, develop efficiently, it is low toxicity, low residual
Stay, inexpensive, environment amenable biological many control fertilizer have turned into the development trend of modern fertilizer.
The content of the invention
It is an object of the invention to provide control fertilizer a kind of liquid compound bio more.
It is a further object to provide the purposes that fertilizer is controlled liquid compound bio more.
It is also another object of the present invention to provide the preparation method that fertilizer is controlled liquid compound bio more.
The microbial inoculum that the present invention is used be China General Microbiological culture presevation administrative center version in 1997 bacterium
The strain disclosed in catalogue is planted, numbering and bacterial strain name are：
Bacterium numbering is CGMCC3.2942 trichoderma viride (Trichoderma viride Persoon ex
Fries), bacterium numbering is CGMCC4.1114 streptomyces hygroscopicus (Streptomyces hygroscopicus Waksman
Et Henrici), bacterium numbering is the CGMCC4.1099 brown streptomycete (Streptomyces of Chinese cassia tree
Cinereohygroscopicus Yan et al.), bacterium numbering is CGMCC1.1226 bacillus amyloliquefaciens
(Bacillus amyloliquefaciens Priest et al.), bacterium numbering is CGMCC1.929 Bacillus sphaericus
(Bacillus sphaericus Meyer et Neide), bacterium numbering is CGMCC1.910 bacillusmusilaginosiengineering
(Bacillus mucilaginosus Krasil, nikov), bacterium numbering is CGMCC1.212 blown-ball Azotobacter
(Azotobacter chroococcum Beijerinck), bacterium numbering is CGMCC4.910 golden streptomyces chromogenes
(Streptomyces aureochromogenes Yan et al.)。
The purpose of the present invention is realized by following technical proposals：
Fertilizer being controlled according to liquid compound bio of the present invention, it is characterised in that it is by following weight more
Raw material components constitute：
1~3 part of the brown strepto- bacterium solution of 1~3 part of streptomyces hygroscopicus liquid, Chinese cassia tree, 2~4 parts of bacillus amyloliquefaciens liquid, spherical bud
2~4 parts of spore bacillus liquid, 7~15 parts of bacillusmusilaginosiengineering liquid, 1~3 part of blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid 1~3
Part, 8~10 parts of the fermentative degradation liquid of trichoderma viride, 8~10 parts of matrix and 45~65 parts of sterilized water,
Wherein, the fermentation process of the fermentative degradation liquid of trichoderma viride is：By weight ratio, 15 parts of solid state rheologies are weighed
Base, is fitted into fermentation vat, adds 9 parts of running water, water content is reached 60%, pH value 7.0 is stirred, and is well mixed, at 121 DEG C
Sterilizing 20 minutes, is down to room temperature, then 3.6 parts of Trichoderma viride strains are inoculated in into solid medium top fermentation, and cultivation temperature 25~
27 DEG C, incubation time 56~72 hours, the degradation product fermented is produced through being sterilized 20 minutes at filtering and 121 DEG C；
Solid medium is discarded after rod is crushed by edible fruits waste residue, straw or edible mushroom and prepared, drinkable water
Fruit waste residue refers to the fruit waste residue of pear cv nanguo, white pear or apple, and straw refers to the stalk of paddy rice, wheat, sorghum or corn,
Edible mushroom discards rod and refers to that mushroom, pleurotus eryngii or the edible mushroom of coprinus comatus discard rod；
Matrix is water-soluble humic acid or one kind or two kinds of mixtures in the feces of livestock and poultry water that becomes thoroughly decomposed；
The feces of livestock and poultry water that becomes thoroughly decomposed refers to the excrement water that becomes thoroughly decomposed of ox, horse or sheep class animal and chicken, duck or bird class；
Sterilized water refers to that seawater and freshwater is 1 according to weight proportion:180~220 mix, and are sterilized at 121 DEG C 20 minutes
Water.
Fertilizer being controlled according to liquid compound bio of the present invention, it is characterised in that it is by following weight more
Raw material components constitute：
2~3 parts of the brown strepto- bacterium solution of 2~3 parts of streptomyces hygroscopicus liquid, Chinese cassia tree, 2~4 parts of bacillus amyloliquefaciens liquid, spherical bud
2~4 parts of spore bacillus liquid, 9~13 parts of bacillusmusilaginosiengineering liquid, 2~3 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid 2~3
Part, 9~10 parts of the fermentative degradation liquid of trichoderma viride, 9~10 parts of matrix and 47~61 parts of sterilized water,
Wherein, the fermentation process of the degradation solution of trichoderma viride is same as above；
Matrix is that just water weight proportion is 1 for water-soluble humic acid and Chicken dung:1~1.2 mixture；
Sterilized water refers to that seawater and freshwater is 1 according to weight proportion:190~210 mix, and are sterilized at 121 DEG C 20 minutes
Water.
According to the purposes that fertilizer is controlled liquid compound bio of the present invention, applied to paddy rice, corn, wheat and fruits and vegetables more
Seed soaking, foliage-spray or pouring root.
According to the preparation method that fertilizer is controlled liquid compound bio of the present invention, it is characterised in that its preparation step more
It is rapid as follows：
A, trichoderma viride fermentative degradation liquid preparation
By weight ratio, 15 parts of solid mediums are weighed, are fitted into fermentation vat, 9 parts of water is added, reaches water content
60%, pH value 7.0 is stirred, and is well mixed, and is sterilized 20 minutes at 121 DEG C, is down to room temperature, then 3.6 parts of Trichoderma viride strains are connect
Plant in solid medium top fermentation, 25~27 DEG C of cultivation temperature, incubation time 56~72 hours, be holding water in fermentation process
Point, and moisture is uniformly distributed, and a small amount of aqua sterilisa was sprayed into solid medium using every two days, keeps water content
60%, and stirred, so that fermentation is complete, in order to ensure the ventilation in fermentation, using four layers of Breathable nonwoven covering cell
Mouthful, the degradation product fermented is produced through being sterilized 20 minutes at filtering and 121 DEG C；
B, various raw material components carry out dispensing by its weight：
Obtained trichoderma viride fermentative degradation liquid is well mixed with described matrix and sterilized water, water suction chain is added
The brown strepto- bacterium solution of mould liquid, Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, brown ball are consolidated
Nitrogen bacterium solution and golden streptomyces chromogenes liquid, are well mixed, and produce and control fertilizer liquid compound bio more；
The weight of wherein raw material components is same as above.
The above-mentioned streptomyces hygroscopicus liquid of the present invention, the brown strepto- bacterium solution of Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus
Liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid and golden streptomyces chromogenes liquid are prepared using conventional method.
1st, for example, a kind of fermentation process for preparing above-mentioned blown-ball Azotobacter liquid：
Preparation technology：Slant strains activation culture-- tapered shaking flask expands culture-- one grade fermemtation tank (seeding tank) liquid
Body spreads cultivation-and-second order fermentation tank spreads cultivation.(each step needs 20~48 hours).
Culture medium：Beef extract-peptone fluid nutrient medium, 30 DEG C of cultivation temperature, rotating speed be 60~120 turns/min, pH be
7.2~7.8, incubation time 20~48 hours, through examining, the quantity of each effective bacterium must not be less than after 0.01 hundred million/mL, containing total
Living bacteria count, can be standby as production bacterium solution up to more than 2.0 hundred million/milliliter (standard GB/T -20287-2006).
Prepare the brown strepto- bacterium solution of Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid and bacillusmusilaginosiengineering liquid
Fermentation process of the fermentation process with above-mentioned blown-ball Azotobacter liquid.
A kind of fermentation process for preparing above-mentioned streptomyces hygroscopicus liquid：
Preparation technology：Slant strains activation culture-- tapered shaking flask expands culture-- one grade fermemtation tank (seeding tank) liquid
Body spreads cultivation-and-second order fermentation tank spreads cultivation.(each step needs 120~144 hours).
Culture medium：Gause I fluid nutrient medium, 28 DEG C of cultivation temperature, rotating speed be 60~120 turns/min, pH be 7.0~
7.2nd, incubation time 120~240 hours, through examining, the quantity of each effective bacterium must not be less than after 0.01 hundred million/mL, containing always having
Viable count is imitated up to more than 2.0 hundred million/milliliter (standard GB/T -20287-2006), can be standby as production bacterium solution.
Prepare fermentation process of the golden streptomyces chromogenes liquid fermentation process with above-mentioned streptomyces hygroscopicus liquid.
The present invention is that, using the trichoderma viride that bacterium numbering is 3.2942, fermented degraded, preparation is easy to crops suction
The carbohydrate of receipts and the trichoderma viride fermentative degradation liquid of organic matter.
The present invention provides efficiently beneficial phosphorus decomposing, potassium decomposing, fixed nitrogen, the microorganism species of disease resisting effect, and life can be played simultaneously
Thing agricultural chemicals, bio-feritlizer and the effect of biological nutrition triple function, but it is nonhazardous, free from environmental pollution.
Tests prove that the microbial strains used have following effect：
Streptomyces hygroscopicus can produce antifungal antibiotic, suppress Rhizoctonia solani Kuhn, Pyricularia oryzae and fusarium graminearum.
The brown streptomycete of Chinese cassia tree is a kind of bacterial manure, can convert n-p-k element in soil, increase soil fertility, and has stimulation to make
Thing growth.
Bacillus amyloliquefaciens can improve crop and endanger germ and adverse circumstance the Scavenging activity that oxygen is produced in primosome, regulation
Cell microorganism environment, maintains the normal physiological metabolism of cell and biochemical reaction, improves the resistance of crop, increases the guarantor of crop
Strong effect, can promote plant growth, improve yield.
Bacillus sphaericus is that a kind of energy produces the bacterium to insect with single-minded insecticidal activity albumen, especially prevents and treats soil
Nematode.
Bacillusmusilaginosiengineering is a kind of B. mucilaginocus, and its effect is that, by the mineral K in soil, being dissolved into can be by plant
The potassium directly absorbed.
Blown-ball Azotobacter is a kind of bacterial fertilizer, can secrete auxin, and it can be straight by the nitrogen in air in process of production
Switch through and turn to the absorbent ammonium nitrogen of crops, nitric nitrogen and organic nitrogen, so as to generate fertilizer efficiency.
Golden streptomyces chromogenes can produce antifungal antibiotic, suppress Rhizoctonia solani Kuhn and Pyricularia oryzae.
The various bacterium solutions are pressed suitable proportion mixture by the present invention, and each Microflora in composite bacteria agent can be kept well
Ratio is stablized relatively.Composite bacteria agent with the microorganism species that are decomposed by natural environment and with without individually cultivating and mixture
Flora compare, using the flora of mixture together again after independent culture, due to rational can be selected according to actual needs
With ratio, each flora biological nature of itself and mutual synergy in microbial inoculum can be fully played, so carrying out biology with it
The scope of application of processing is widely.
Fertilizer being controlled liquid compound bio prepared by the present invention, containing total living bacteria count up to more than 2.0 hundred million/milliliter (country more
Standard GB-20287-2006).
The present invention utilizes trichoderma viride, using solid-state aerobic fermentation, degraded edible fruits waste residue, straw or edible
Bacterium discards polysaccharide, carbohydrate and cellulose in rod, introduces efficiently beneficial phosphorus decomposing, potassium decomposing, fixed nitrogen, anti-disease microbial flora,
Also, by the control to fermentation condition, the nutriment in a fertilizer of control fertilizer more than biology is reached highest level.Simultaneously play biological pesticide,
Biological fertilizer efficiency and the effect of biological nutrition triple function, also, it is nonhazardous, free from environmental pollution.
Especially, the present invention discards rod fermentative degradation using pear cv nanguo waste residue, rice straw or Lenlinus edodes, with fixed nitrogen, solution
Phosphorus, the active complex microorganism of potassium decomposing, and to rice blast, bakanae disease of rice and rice sheath blight disease, wheat scab, wheat root
Maize ear rot, the leaf blight of corn, corn southern leaf blight have compound actinomycete agent and the mixing of composite bacterial microbial inoculum of antagonism, not only use
In improving edaphon colony structure, activating soil nutrient improves The Rhizosphere of Rice nutrition, improves yield and quality of rice, change
Kind water quality, can more promote the growth of aquatic in rice terrace.
Fertilizer is controlled the liquid compound bio of the present invention more, being used to soaking seed through field, foliage-spray or pouring root contrast test, is taken
Obtain positive effect as follows：
(1) containing abundant organic matter and humic acid, soil texture can be improved, vegetable soil promotes soil to build up fertility.
(2) with waiting the other fertilizer ratios of nutrient, crop yield 20%.
(3) formulate and coordinate crops and absorb nutrition, stimulate and regulation and control plant growth, reduce or reduction crop disease and insect,
Influence crop quality.
(4) crab, loach, swamp eel net gain 10% cultivated in rice terrace is made.
(5) without or reduce fertilizer amount, beneficial to environmental protection.
Embodiment
Following embodiment is only used for that the present invention is explained, and is not construed as limiting the scope of the claims restriction,
Other alternative means that those skilled in the art are contemplated that according to description, all should the claims in the present invention protection
Within the scope of.
The microorganism fungus kind that the embodiment of the present invention is utilized, for the trichoderma viride of fermentative degradation, and it is phosphorus decomposing, potassium decomposing, solid
The microorganism species of nitrogen, disease resisting effect, select the strain catalogue of China General Microbiological culture presevation administrative center version in 1997
Disclosed in strain.
It is numbered and bacterial strain name is：Bacterium numbering is CGMCC3.2942 trichoderma viride (Trichoderma viride
Persoon ex Fries), bacterium numbering is CGMCC4.1114 streptomyces hygroscopicus (Streptomyces
Hygroscopicus Waksman et Henrici), bacterium numbering is the CGMCC4.1099 brown streptomycete of Chinese cassia tree
(Streptomyces cinereohygroscopicus Yan et al.), bacterium numbering is CGMCC1.1226 solution starch
Bacillus (Bacillus amyloliquefaciens Priest et al.), bacterium numbering is spherical for CGMCC1.929's
Bacillus (Bacillus sphaericus Meyer et Neide), bacterium numbering is CGMCC1.910 gel-shaped gemma
Bacillus (Bacillus mucilaginosus Krasil, nikov), bacterium numbering is CGMCC1.212 blown-ball Azotobacter
(Azotobacter chroococcum Beijerinck), bacterium numbering is CGMCC4.910 golden streptomyces chromogenes
(Streptomyces aureochromogenes Yan et al.)。
Control the preparation method of fertilizer more the liquid compound bio of the embodiment of the present invention 1~9, be following steps：
A, trichoderma viride fermentative degradation liquid preparation
By weight ratio, 15 parts of solid mediums are weighed, are fitted into fermentation vat, 9 parts of water is added, reaches water content
60%, pH value 7.0 is stirred, and is well mixed, and is sterilized 20 minutes at 121 DEG C, is down to room temperature, then 3.6 parts of Trichoderma viride strains are connect
Plant in solid medium top fermentation, 25 DEG C of cultivation temperature, incubation time 60 hours, be holding moisture, Yi Jishui in fermentation process
That divides is uniformly distributed, and a small amount of aqua sterilisa was sprayed into solid medium using every two days, water content is kept 60%, and stirred
Mix, so that fermentation is complete, in order to ensure the ventilation in fermentation, using four layers of Breathable nonwoven covering cell mouthful, the drop fermented
Thing is solved through being sterilized 20 minutes at filtering and 121 DEG C, is produced；
B, various raw material components carry out dispensing according to the weight described in embodiment：
By trichoderma viride fermentative degradation liquid produced above and described matrix and sterilized water, it is well mixed, adds
The brown strepto- bacterium solution of streptomyces hygroscopicus liquid, Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid,
Blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid, are well mixed, and produce and control fertilizer liquid compound bio more.
Solid medium is to be discarded after rod is crushed to prepare by edible fruits waste residue, straw or edible mushroom in preparation method
, edible fruits waste residue refers to the waste residue of pear cv nanguo, and straw refers to the stalk of paddy rice, and edible mushroom discards rod and refers to Lenlinus edodes
Discarded rod；
Matrix refers to that just water is 1 according to weight proportion to water-soluble humic acid with Chicken dung:1 mixture；
Sterilized water refers to that seawater and freshwater is 1 according to weight proportion:200 mix, the water of sterilizing 20 minutes at 121 DEG C.
The brown strepto- bacterium solution of the above-mentioned streptomyces hygroscopicus liquid of the embodiment of the present invention 1~9, Chinese cassia tree, bacillus amyloliquefaciens liquid, ball
Shape bacillus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter liquid, the preparation side of the respective zymotic fluid of golden streptomyces chromogenes liquid
Method is as follows：
Preparing the fermentation process of above-mentioned blown-ball Azotobacter liquid is：Beef extract-peptone liquid culture medium, cultivation temperature 30 DEG C, turn
Speed is 60 turns/and min, pH be 7.5, incubation time 25 hours, and through examining, through examining, the quantity of each effective bacterium must not be less than
After 0.01 hundred million/mL, containing total living bacteria count up to more than 2.0 hundred million/milliliter (standard GB/T -20287-2006), production can be used as
Bacterium solution is standby.
Prepare the brown strepto- bacterium solution of Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid and bacillusmusilaginosiengineering liquid
Fermentation process of the fermentation process with above-mentioned blown-ball Azotobacter liquid.
Preparing the fermentation process of above-mentioned streptomyces hygroscopicus liquid is：Gause I liquid culture medium, 28 DEG C of cultivation temperature, rotating speed are
60 turns/min, pH be 7.0, incubation time 240 hours, through examining, the quantity of each effective bacterium must not be less than 0.01 hundred million/mL
Afterwards, can be standby as production bacterium solution containing total living bacteria count up to more than 2.0 hundred million/milliliter (standard GB/T -20287-2006).
Prepare fermentation process of the golden streptomyces chromogenes liquid fermentation process with above-mentioned streptomyces hygroscopicus liquid.
Embodiment 1：
Solid medium be 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix made from dry pear cv nanguo waste residue, it is sterile
53.2 parts of water, is well mixed, and mixed liquor is made.Again by 2.5 parts of streptomyces hygroscopicus liquid, 2.5 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch
3 parts of bacillus liquid, 3 parts of Bacillus sphaericus liquid, 11 parts of bacillusmusilaginosiengineering liquid, 2.4 parts of blown-ball Azotobacter liquid, gold are chromogenic
2.4 parts of strepto- bacterium solution is well mixed with above-mentioned mixed liquor, obtain containing living bacteria count be more than 2.0 hundred million/gram liquid compound bio
Many control fertilizer.For wheat paddock, by 1：300~500 times of dilutions spray plant, and every mu of ground is every time with 300~500mL.Sprinkling
Period productive phase, every sprinkling in 7 days or so once, continuously using 2~3 times, can receive substantially in nursery, setting date, initial bloom stage
Effect, plays the fertile double effects of medicine.
Embodiment 2：
Solid medium be 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix made from dry pear cv nanguo waste residue, it is sterile
47 parts of water, is well mixed, and mixed liquor is made.Again by 3 parts of streptomyces hygroscopicus liquid, 3 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch gemma bar
4 parts of bacterium solution, 4 parts of Bacillus sphaericus liquid, 13 parts of bacillusmusilaginosiengineering liquid, 3 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid
3 parts, be well mixed with above-mentioned mixed liquor, can obtain containing living bacteria count be more than 2.0 hundred million/gram liquid compound bio more control fertilizer
Material, for rice terrace, rice paddy seed is soaked 2.5kg7 days using this product 0.5kg, and fertile medicine economic benefits and social benefits are played in 25~30 DEG C of vernalization sowings
Effect.
Embodiment 3：
Solid medium be 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix made from dry pear cv nanguo waste residue, it is sterile
59 parts of water, is well mixed, and mixed liquor is made.Again by 2 parts of streptomyces hygroscopicus liquid, 2 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch gemma bar
2 parts of bacterium solution, 2 parts of Bacillus sphaericus liquid, 9 parts of bacillusmusilaginosiengineering liquid, 2 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid 2
Part, be well mixed with above-mentioned mixed liquor, can obtain containing living bacteria count about 2.0 hundred million/gram liquid compound bio more control fertilizer.
For corn field, by 1：300~500 times of dilutions spray plant, and 300~500mL of this product is used on every mu of ground every time.Sprinkling period can
In nursery, setting date, initial bloom stage, every sprinkling in 7 days or so once, continuously using 2~3 times, receives positive effect at productive phase,
Play the fertile double effects of medicine.
Embodiment 4：
Solid medium is that dried thin mushroom bacterium discards 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, nothing made from rod
53.2 parts of bacterium water, is well mixed, and mixed liquor is made.Again by 2.5 parts of streptomyces hygroscopicus liquid, 2.5 parts of the brown strepto- bacterium solution of Chinese cassia tree, Xie Dian
3 parts of afnyloliquefaciens liquid, 3 parts of Bacillus sphaericus liquid, 11 parts of bacillusmusilaginosiengineering liquid, 2.4 parts of blown-ball Azotobacter liquid, gold production
2.4 parts of color strepto- bacterium solution is well mixed with above-mentioned mixed liquor, obtain being more than 2.0 hundred million containing living bacteria count/gram the compound life of liquid
Fertilizer is controlled thing more.For wheat paddock, by 1：300~500 times of dilutions spray plant, and every mu of ground is every time with 300~500mL.Spray
Period is spilt for nursery, setting date, initial bloom stage is productive phase, every sprinkling in 7 days or so once, continuous to use 2-3 times, receives substantially
Effect, plays the fertile double effects of medicine.
Embodiment 5：
Solid medium is that dried thin mushroom bacterium discards 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, nothing made from rod
47 parts of bacterium water, is well mixed, and mixed liquor is made.Again by 3 parts of streptomyces hygroscopicus liquid, 3 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch gemma
4 parts of bacillus liquid, 4 parts of Bacillus sphaericus liquid, 13 parts of bacillusmusilaginosiengineering liquid, 3 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes
3 parts of liquid, is well mixed with above-mentioned mixed liquor, can obtain containing living bacteria count be more than 2.0 hundred million/gram liquid compound bio more control fertilizer
Material.For rice terrace, soak rice paddy seed 2.5kg7 days using this product 0.5kg, sowed after 25~30 DEG C of vernalization, play fertile medicine double
The effect of effect.
Embodiment 6：
Solid medium is that dried thin mushroom bacterium discards 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, nothing made from rod
59 parts of bacterium water, is well mixed, and mixed liquor is made.Again by 2 parts of streptomyces hygroscopicus liquid, 2 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch gemma
2 parts of bacillus liquid, 2 parts of Bacillus sphaericus liquid, 9 parts of bacillusmusilaginosiengineering liquid, 2 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes
2 parts of liquid, is well mixed with above-mentioned mixed liquor, can obtain containing living bacteria count about 2.0 hundred million/gram liquid compound bio more control fertilizer
Material.For corn field, by 1：300~500 times of dilutions spray plant, and every mu of ground is every time with 300~500mL.Sprinkling period is
Nursery, setting date, initial bloom stage, every sprinkling in 7 days or so once, continuously using 2~3 times, receives positive effect, risen productive phase
To the fertile double effects of medicine.
Embodiment 7：
Solid medium is 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, sterilized water made from solid carbon dioxide rice straw
53.2 parts, it is well mixed, mixed liquor is made.Again by 2.5 parts of streptomyces hygroscopicus liquid, 2.5 parts of the brown strepto- bacterium solution of Chinese cassia tree, solution starch bud
3 parts of spore bacillus liquid, 3 parts of Bacillus sphaericus liquid, 11 parts of bacillusmusilaginosiengineering liquid, 2.4 parts of blown-ball Azotobacter liquid, golden chromogenic chain
2.4 parts of mould liquid is well mixed with above-mentioned mixed liquor, obtain containing living bacteria count be more than 2.0 hundred million/gram liquid compound bio it is many
Control fertilizer.For wheat paddock, by 1：300-500 times of dilution sprays plant, and every mu of ground is every time with 300~500mL.Sprinkling period
For nursery, setting date, initial bloom stage, every sprinkling in 7 days or so once, continuously using 2~3 times, receives positive effect at productive phase,
Play the fertile double effects of medicine.
Embodiment 8：
Solid medium is 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, sterilized water made from solid carbon dioxide rice straw
47 parts, it is well mixed, mixed liquor is made.Again by 3 parts of streptomyces hygroscopicus liquid, 3 parts of the brown strepto- bacterium solution of Chinese cassia tree, bacillus amyloliquefaciens
4 parts of liquid, 4 parts of Bacillus sphaericus liquid, 13 parts of bacillusmusilaginosiengineering liquid, 3 parts of blown-ball Azotobacter liquid, golden streptomyces chromogenes liquid 3
Part, be well mixed with above-mentioned mixed liquor, can obtain containing living bacteria count more than 2.0 hundred million/gram liquid compound bio more control fertilizer,
For rice terrace, soak rice paddy seed 2.5kg7 days using this product 0.5kg, 25~30 DEG C of vernalization can be sowed, play fertile medicine double
The effect of effect.
Embodiment 9：
Solid medium is 10 parts of trichoderma viride fermentative degradation liquid, 10 parts of matrix, sterilized water made from solid carbon dioxide rice straw
59 parts, it is well mixed, mixed liquor is made.
Again by 2 parts of streptomyces hygroscopicus liquid, 2 parts of the brown strepto- bacterium solution of Chinese cassia tree, 2 parts of bacillus amyloliquefaciens liquid, spherical gemma bar
2 parts of bacterium solution, 9 parts of bacillusmusilaginosiengineering liquid, 2 parts of blown-ball Azotobacter liquid, golden 2 parts of streptomyces chromogenes liquid, it is mixed with above-mentioned mixed liquor
Close uniform, can obtain containing living bacteria count about 2.0 hundred million/gram liquid compound bio more control fertilizer.For corn field, by 1：
300-500 times of dilution sprays plant, and every mu of ground is every time with 300~500mL.Sprinkling period be nursery, setting date, initial bloom stage,
Productive phase, every sprinkling in 7 days or so once, continuously using 2~3 times, positive effect is received, play the fertile double effects of medicine.
Comparative example 1：The composite microbiological fertilizer produced for Hong Kong Bao Nong bio tech ltd.
Comparative example 2：For the Enteromorpha bio-bacterial manure produced with Qingdao Seawin Biotech Group Co., Ltd..
Comparative example 3：The compound bacteria seed manure combined for Japanese silicate bacteria with Taiwan promise Cattell actinomyces.
Main experimental conditions, method that fertilizer passes through field comparison test are controlled the compound bio of the embodiment of the present invention 1~9 more
It is as follows with result：
Rice test：In Donggang City Chang Mount town hole village, excellent No. one of kind New Port, 5 points of samplings of diagonal, every 1m2, it is real
Row actual measurement, rice yield is calculated by, and 300 times of+200 times of sprayings are soaked seed 7 days；Control group CK areas.
Corn trials:In Haicheng City Datun Town Yao Tun villages experimental plot, 5 points of samplings of diagonal, 40 plants every are produced in terms of putting, and 300
+ 200 times of spraying is soaked seed 5 days again；Control group CK areas.
Wheat is tested：In Hebei Jizhou City experimental plot, 5 points of samplings of diagonal, 40 plants every are produced in terms of putting, 300 times of sprayings+
200 times are soaked seed 5 days；Control group CK areas.
Capsicum, eggplant, tomato experiment：5 points of samplings of diagonal, 6 plants every are produced in terms of putting ,+200 times of seed soaking 5 of 300 times of sprayings
My god；Control group CK areas.
Kyoto grape is tested：In Haicheng City great Tun vineyaries, the equidistant sampling of parallel lines, totally 5 points, 2 plants every are produced in terms of putting,
300 times of+200 times of spraying pouring roots 5 days；Control group CK areas.
The embodiment of table 1 and comparative example to soil layer construction, organic matter, soil pH influence situation：
The average product situation of the embodiment of table 2 and the comparative example of grade nutrient：
The embodiment of table 3 and average product situation of the grade nutrient comparative example to cereal crops：
The embodiment of table 4 is with waiting influence situation of the nutrient comparative example to cereal crops quality：
The embodiment of table 5 and qualitative effects situation of the grade nutrient comparative example to fruit trees and vegetables：
Embodiment 2,5 and 8 is applied to rice terrace, is compared with using common fertilizer, makes the crab, loach, swamp eel of cultivation net
Weightening 8~12%.
The shelf life of products of the embodiment of the present invention is 12~18 months.
Claims (4)
1. controlling fertilizer a kind of liquid compound bio, it is characterised in that it is made up of the raw material components of following weight more：
1~3 part of the brown strepto- bacterium solution of 1~3 part of streptomyces hygroscopicus liquid, Chinese cassia tree, 2~4 parts of bacillus amyloliquefaciens liquid, spherical gemma bar
It is 2~4 parts of bacterium solution, 7~15 parts of bacillusmusilaginosiengineering liquid, 1~3 part of blown-ball Azotobacter liquid, golden 1~3 part of streptomyces chromogenes liquid, green
45~65 parts of 8~10 parts of the fermentative degradation liquid of color Trichoderma, 8~10 parts of matrix and sterilized water,
Wherein, the fermentation process of the fermentative degradation liquid of trichoderma viride is that by weight ratio, 3.6 parts of Trichoderma viride strains are connect
Kind on 15 parts of solid mediums, make water content be 60%, 25~27 DEG C of cultivation temperature, pH value 7.0, incubation time 56~72 is small
When 121 DEG C at sterilize 20 minutes, produce；
Solid medium is discarded after rod is crushed by edible fruits waste residue, straw or edible mushroom and prepared, and edible fruits give up
Slag refers to the fruit waste residue of pear cv nanguo, white pear or apple, and straw refers to the stalk of paddy rice, wheat, sorghum or corn, eats
Bacterium discards rod and refers to that mushroom, pleurotus eryngii or the edible mushroom of coprinus comatus discard rod；
Matrix is water-soluble humic acid or one kind or two kinds of mixtures in the feces of livestock and poultry water that becomes thoroughly decomposed；
The feces of livestock and poultry water that becomes thoroughly decomposed refers to the excrement water that becomes thoroughly decomposed of ox, horse or sheep class animal and chicken, duck or bird class；
Sterilized water refers to that seawater and freshwater is 1 according to weight proportion:180~220 mixing and the water of sterilizing.
2. controlling fertilizer liquid compound bio according to claim 1, it is characterised in that it is by following weight more
Raw material components constitute：
2~3 parts of the brown strepto- bacterium solution of 2~3 parts of streptomyces hygroscopicus liquid, Chinese cassia tree, 2~4 parts of bacillus amyloliquefaciens liquid, spherical gemma bar
It is 2~4 parts of bacterium solution, 9~13 parts of bacillusmusilaginosiengineering liquid, 2~3 parts of blown-ball Azotobacter liquid, golden 2~3 parts of streptomyces chromogenes liquid, green
47~61 parts of 9~10 parts of the fermentative degradation liquid of color Trichoderma, 9~10 parts of matrix and sterilized water,
Wherein, the fermentation process of the degradation solution of trichoderma viride is with described in claim 1；
Matrix is that just water weight proportion is 1 for water-soluble humic acid and Chicken dung:1~1.2 mixture；
Sterilized water refers to that seawater and freshwater is 1 according to weight proportion:190~210 mix, the water of sterilizing 20 minutes at 121 DEG C.
3. the purposes of fertilizer being controlled liquid compound bio according to claim 1, applied to paddy rice, corn, wheat and fruit more
Seed soaking, pouring root or the foliage-spray of vegetable.
4. the preparation method of fertilizer being controlled liquid compound bio according to claim 1, it is characterised in that its preparation step more
It is rapid as follows：
A, trichoderma viride fermentative degradation liquid preparation：
By weight ratio, 15 parts of solid mediums are weighed, are fitted into fermentation vat, 9 parts of water is added, water content is reached 60%,
PH value 7.0, is stirred, and is well mixed, and is sterilized 20 minutes at 121 DEG C, is down to room temperature, then 3.6 parts of Trichoderma viride strains are inoculated in
Solid medium top fermentation, 25~27 DEG C of cultivation temperature, incubation time 56~72 hours, in fermentation process for keep moisture and
Being uniformly distributed for moisture, a small amount of aqua sterilisa was sprayed into solid medium using every two days, water content is kept 60%, and be subject to
Stirring, so that fermentation is complete, in order to ensure the ventilation in fermentation, using four layers of Breathable nonwoven covering cell mouthful, is fermented
Degradation product is produced through being sterilized 20 minutes at filtering and 121 DEG C；
B, various raw material components carry out dispensing by its weight：
Obtained trichoderma viride fermentative degradation liquid is well mixed with described matrix and sterilized water, streptomyces hygroscopicus is added
The brown strepto- bacterium solution of liquid, Chinese cassia tree, bacillus amyloliquefaciens liquid, Bacillus sphaericus liquid, bacillusmusilaginosiengineering liquid, blown-ball Azotobacter
Liquid and golden streptomyces chromogenes liquid, are well mixed, and produce and control fertilizer liquid compound bio more；
Wherein the weight of raw material components is with claim 1.
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| CN104892177A (en) * | 2015-06-03 | 2015-09-09 | 张亚鹏 | Special microbial manure for apple trees and preparation method thereof |
| CN105175085B (en) * | 2015-06-15 | 2018-05-29 | 山西浩之大生物科技有限公司 | A kind of method for the kuh-seng dregs of a decoction organic fertilizer that high bioactivity is prepared using Trichoderma |
| CN105130652A (en) * | 2015-09-09 | 2015-12-09 | 张乔木 | Organic composite water soluble fertilizer and preparation method thereof |
| CN105347926A (en) * | 2015-11-10 | 2016-02-24 | 绥化市凯华农业科技有限公司 | Preparation method for microbial organic soil bactericidal and insecticidal warming conditioning agent |
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| CN106397030A (en) * | 2016-08-31 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Composite microbial fertilizer and preparation method thereof |
| CN106811433A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect |
| CN108260482A (en) * | 2018-03-22 | 2018-07-10 | 佛山推启农业研究院（普通合伙） | A kind of campanulaceae non-polluted planting method rich in various trace elements |
| CN112624879B (en) * | 2020-12-30 | 2022-11-29 | 北京嘉博文生物科技有限公司 | Tomato soil conditioner and application thereof |
| CN115250839A (en) * | 2022-06-30 | 2022-11-01 | 辽宁工程技术大学 | Efficient wind prevention and sand fixation method |
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| CN1301220C (en) * | 2001-01-23 | 2007-02-21 | 任啟刚 | Feces and urine treatment method by compound microbe |
| CN101186879B (en) * | 2007-12-05 | 2010-07-14 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial agent |
| CN101357857B (en) * | 2008-09-05 | 2010-08-25 | 辽宁科技大学 | Bio organic fertilizer for aquaculture and paddy field and producing method thereof |
| CN102718603B (en) * | 2012-07-11 | 2013-10-30 | 德州市元和农业科技开发有限责任公司 | Compound microbial fertilizer and preparation method thereof |
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