CN109135762A - A kind of microbial-bacterial fertilizer and preparation method thereof for promoting tangerine orange to grow - Google Patents
A kind of microbial-bacterial fertilizer and preparation method thereof for promoting tangerine orange to grow Download PDFInfo
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Abstract
The invention discloses a kind of microbial-bacterial fertilizers and preparation method thereof for promoting tangerine orange to grow, which includes following component: 15 mass parts~25 mass parts microbial inoculum；20 mass parts~30 mass parts soy meal；10 mass parts~15 mass parts sucrose；5 mass parts~10 mass parts auxiliary agent；The preparation method includes the following steps: step 1,15 mass parts~25 mass parts microbial inoculum, 20 mass parts~30 mass parts soy meal, 10 mass parts~15 mass parts sucrose, 5 mass parts~10 mass parts auxiliary agent are uniformly mixed, mixture is obtained；Step 2, the water that 30 times of quality are added in the mixture is dissolved, and is then fermented in 30 DEG C of gnotobasis, is obtained fermentation liquid；Step 3,2m is passed through in fermentation liquid3The air of/h/L, and fermentation liquid is placed in upper, middle and lower respectively has in blender there are two blade and be stirred, 18~20h is cultivated under the revolving speed of 60r/min, obtains microbial-bacterial fertilizer.
Description
Technical field
The invention belongs to the technical fields of plant microorganism fertilizer, and in particular to a kind of micro- life for promoting tangerine orange to grow
Object bacterial manure and preparation method thereof.
Background technique
Tangerine orange is the excellent variety of China's citrus, is important one of the industrial crops in southern drug in some provinces, for pushing area
Domain expanding economy realizes that increasing peasant income play an important role.Tangerine orange Orchard Soil is the medium of tangerine orange plant existence, in soil
The elements such as organic matter, nitrogen, phosphorus, potassium be main nutrient elements in soil, be the energy of edaphon, soil can be driven
The conversion and circulation of the nutrients such as middle carbon, nitrogen, phosphorus and potassium, influence the physicochemical property of soil, directly affect tangerine orange growth and development and
Absorption function.Since chemical fertilizer is largely applied in tangerine orange garden for a long time, lead to soil hardening, eutrophication, destroys soil texture and micro-
The stability of ecological environment, poisonous and harmful substance increase, and soil fertility decline, plant is subject to pest and disease damage.
Currently, most of the method for increasing fertility of land all is by imposing chemical fertilizer or organic fertilizer, imposing chemical fertilizer be will form
A kind of vicious circle, although soil fertility can be restored by imposing organic fertilizer, poisonous and harmful substance etc. in the soil that cannot degrade, and be easy
As plant by the inducement of pest and disease damage.
Summary of the invention
The present invention is to carry out to solve the above-mentioned problems, and it is an object of the present invention to provide a kind of micro- for promoting tangerine orange to grow
Bio-bacterial manure and preparation method thereof, the problem of being able to solve soil hardening, additionally it is possible to it increases fertility of land, enhancing plant pair pest and disease damage
Resistance.
The present invention provides a kind of microbial-bacterial fertilizers for promoting tangerine orange to grow, and have the feature that, including following
Ingredient:
Wherein, microbial inoculum by mass ratio be 5:4:1 0.1% streptomyces microflavus bacterium solution, 0.2% gemma bar
Bacterium bacterium solution and 0.05% phosphate-solubilizing bacteria bacterium solution are constituted.
In the microbial-bacterial fertilizer provided by the present invention for promoting tangerine orange growth, it can also have the following features:
In, bacillus bacterium solution by mass ratio be the bacillus subtilis of 1:1:1:1, bacillus laterosporus, Bacillus polymyxa and
Bacillus licheniformis is formulated.
In the microbial-bacterial fertilizer provided by the present invention for promoting tangerine orange growth, it can also have the following features:
In, auxiliary agent include mass ratio be the 0.8% dibastic sodium phosphate solution of 2:2:1:1,0.4% ammonium sulfate, 0.15% chlorine
Change the calcium chloride solution of ammonium salt solution, 0.13%.
The present invention also provides a kind of preparation methods of microbial-bacterial fertilizer for promoting tangerine orange to grow, and have such spy
Sign, includes the following steps:
Step 1, by 15 mass parts~25 mass parts microbial inoculum, 20 mass parts~30 mass parts soy meal, 10
Mass parts~15 mass parts sucrose, 5 mass parts~10 mass parts auxiliary agent are uniformly mixed, and obtain mixture；
Step 2, the water that 30 times of quality are added in the mixture is dissolved, and is then sent out in 30 DEG C of gnotobasis
Ferment obtains fermentation liquid；
Step 3,2m is passed through in fermentation liquid3The air of/h/L, and fermentation liquid is placed in upper, middle and lower and is respectively had there are two blade
Blender in be stirred, under the revolving speed of 60r/min cultivate 18~20h, obtain microbial-bacterial fertilizer.
In the preparation method provided by the present invention for promoting the microbial-bacterial fertilizer of tangerine orange growth, can also have in this way
Feature: where in step 1, the preparation of microbial inoculum includes following sub-step:
Step 1-1 takes 200 grams of peeled potatoes, is cut into small pieces, and adds 1000 milliliters of water, boils 30 minutes, filters off potato
Filtrate is then complemented to 1000 milliliters by block, is added 20 grams of glucose, 15 grams of agar, after dissolution, is fitted into eggplant-shape bottle, sterilizing 30
Minute, obtain culture medium；
A certain amount of thin yellow chain certain kind of berries bacterium is added in the medium, in 36 DEG C of at a temperature of 14~18h of culture, obtains 0.1%
Thin yellow chain certain kind of berries bacterium bacterium solution；
Step 1-3, respectively by a certain amount of bacillus subtilis, a certain amount of bacillus laterosporus, a certain amount of mostly glutinous
Bacillus, a certain amount of bacillus licheniformis are placed in respective nutrient agar, train under the conditions of temperature is 30 DEG C
14~18h is supported, prepares and obtains bacillus subtilis bacterium solution, bacillus laterosporus bacterium solution, mostly glutinous bud that mass concentration is 0.2%
Spore bacillus bacterium solution, Bacillus licheniformis liquid, then by bacillus subtilis bacterium solution, bacillus laterosporus bacterium solution, mostly glutinous gemma
Bacillus bacterium solution and Bacillus licheniformis liquid are mixed according to the mass ratio of 1:1:1:1, obtain 0.2% bacillus bacterium
Liquid.
In the preparation method provided by the present invention for promoting the microbial-bacterial fertilizer of tangerine orange growth, can also have in this way
Feature: where in step 1-3, the culture medium of bacillus subtilis is the yeast extract dissolution by 10 grams of peptone and 5 grams
In the formation of 1000 milliliters of water,
The culture medium of bacillus laterosporus is by 10 grams of peptone, 3 grams of beef extract and the dissolution of 3 grams of sodium chloride
In the formation of 1000 milliliters of water,
The culture medium of Bacillus polymyxa be by 17.5 grams of soluble starch, 25 grams of peptone and 1.25 gram oxygen
Change sodium is dissolved in the formation of 1000 milliliters of water,
The culture medium of bacillus licheniformis is molten by 10 grams of peptone, 15 grams of beef extract and 15 grams of sodium chloride
Solution is in the formation of 1000 milliliters of water.
The action and effect of invention
A kind of microbial-bacterial fertilizer and preparation method thereof for promoting tangerine orange to grow, the preparation involved according to the present invention
Method and process process is simple, short processing time.In addition, containing auxin, antibacterial in the metabolite of the streptomyces microflavus used
Growth regulator ingredient necessary to the plant growths such as element, phenylacetic acid, succinic acid and the basic element of cell division has in conversion soil
Nitrogen, phosphorus, potassium, increase soil fertility, and inhibit germ reproduction, and diseases prevention keeps a full stand of seedings, and stimulate cell division, promote crop take root, germinate, at
It is ripe, promote active principle synthesis the effect of；And the subtilin that is generated in bacillus subtilis growth course, polymyxins,
Nystatin, gramicidins isoreactivity substance, these active materials have significantly the pathogenic bacteria of pathogenic bacteria or autogenous infection
Inhibiting effect；Bacillus laterosporus can solidify several heavy metals, reduce plant in-vivo heavy metal content；Bacillus polymyxa is to thin
Bacterium property soil-borne disease-- plant-bacterial-wilt has good control efficiency；Bacillus licheniformis can generate resistant activity material, and
With unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited.
Therefore, a kind of preparation method of microbial-bacterial fertilizer for promoting tangerine orange to grow of the invention is prepared micro-
Bio-bacterial manure is improving soil microstructure, decomposes the soil organism and toxin, while enhancing plant resistance, moreover it is possible to effectively subtract
The generation of few pest and disease damage, reduces the use of pesticide, promotes quality of agricultural product and mouthfeel, and environmentally friendly, soil can be inhibited true
Bacterium growth, diseases prevention keep a full stand of seedings, convert the elements such as nitrogen, phosphorus, potassium in soil, improve the fertility of soil, and irritating plant growth
Effect.
Specific embodiment
In order to which the technological means for realizing the present invention is easy to understand with effect, the present invention is made with reference to embodiments
It is specifically described.
Embodiment one:
Step 1, by the microbial inoculum of 15 mass parts, the soy meal of 20 mass parts, the sucrose of 10 mass parts, 5 mass parts
Auxiliary agent be uniformly mixed, obtain mixture.
Step 1-1 takes 200 grams of peeled potatoes, is cut into small pieces, and adds 1000 milliliters of water, boils 30 minutes, filters off potato
Filtrate is then complemented to 1000 milliliters by block, is added 20 grams of glucose, 15 grams of agar, after dissolution, is fitted into eggplant-shape bottle, sterilizing 30
Minute, obtain culture medium.
A certain amount of thin yellow chain certain kind of berries bacterium is added in step 1-2 in the medium, 36 DEG C at a temperature of cultivate 14h, obtain
0.1% thin yellow chain certain kind of berries bacterium bacterium solution.
Streptomyces microflavus can be isolated from tangerine orange Orchard Soil, method particularly includes:
Step 1-2-1, selection arrive alkalescence, organic matter soil abundant compared with drying, good permeability, neutrality, first scalp table
Layer soil, takes tens of grams of soil of 10~30 centimetres of depths, is packed into kraft envelope, seals sack；Slowly drained soil is encountered preferably to be packed into
In polybag or aluminium box, the soil sampling adopted back need to be separated in time.
Step 1-2-2, soil mortar is finely ground, 60 mesh sieving take a certain amount of fine earth to be laid in sterilized smooth hard
On cardboard, paper product is slightly larger than the diameter of culture dish, soil extra on smooth hardboard is inclined, seeing on cardboard has one layer of fine earth
Grain adheres to then ideal, when inoculation, the ware lid of good culture medium is opened slightly, downwards gently by soil cardboard clay
Insertion covering thereon, touches cardboard with ware lid slightly and extracts out at once, cover ware lid.
Above-mentioned inoculated culture dish is inverted in 30 DEG C of constant incubators and cultivates, separates after 3~4 days by step 1-2-3
Streptomyces microflavus strain out, then purifying culture 7~14 days is carried out, obtain streptomyces microflavus.
Step 1-3, respectively by a certain amount of bacillus subtilis, a certain amount of bacillus laterosporus, a certain amount of mostly glutinous
Bacillus, a certain amount of bacillus licheniformis are placed in respective nutrient agar, train under the conditions of temperature is 30 DEG C
14~18h is supported, prepares and obtains bacillus subtilis bacterium solution, bacillus laterosporus bacterium solution, mostly glutinous bud that mass concentration is 0.2%
Spore bacillus bacterium solution, Bacillus licheniformis liquid, then by bacillus subtilis bacterium solution, bacillus laterosporus bacterium solution, mostly glutinous gemma
Bacillus bacterium solution and Bacillus licheniformis liquid are mixed according to the mass ratio of 1:1:1:1, obtain 0.2% bacillus bacterium
Liquid.
In step 1-3, the culture medium of bacillus subtilis is dissolved in by 10 grams of peptone and 5 grams of yeast extract
The formation of 1000 milliliters of water, the culture medium of bacillus laterosporus is by 10 grams of peptone, 3 grams of beef extract and 3 grams
Sodium chloride be dissolved in 1000 milliliters water formation, the culture medium of Bacillus polymyxa is formed sediment by 17.5 grams of solubility
Powder, 25 grams of peptone and 1.25 gram sodium oxide molybdena be dissolved in 1000 milliliters water formation, the training of bacillus licheniformis
Feeding base be dissolved in by 10 grams of peptone, 15 grams of beef extract and 15 grams of sodium chloride 1000 milliliters water formation.
Step 2, the water that 30 times of quality are added in the mixture is dissolved, and is then sent out in 30 DEG C of gnotobasis
Ferment obtains fermentation liquid.
Step 3,2m is passed through in fermentation liquid3The air of/h/L, and fermentation liquid is placed in upper, middle and lower and is respectively had there are two blade
Blender in be stirred, cultivate 18h under the revolving speed of 60r/min, obtain microbial-bacterial fertilizer.
The dosage form of the microbial-bacterial fertilizer is liquid microbial inoculum, keeps it good with soil compatibility, and fertilizer is quick, and utilization rate is high,
Both it can be directly sprayed on plant blade face, plant root can also be applied to.
The microbial-bacterial fertilizer is used for 4 years 14 plants of raw honey tangerine plant, monthly average growth rate is 6.25%~6.74%,
Monthly average growth rate 1.72%~3.58% of 2 plants higher than blank control, as shown in table 1.
The moon growth rate of tangerine orange plant after the microbial-bacterial fertilizer of the application embodiment one of table 1
The action and effect of embodiment
By embodiment one it is found that after imposing on 4 years raw honey tangerine plant using the microbial-bacterial fertilizer that the present invention is prepared, tangerine orange
It is monthly average growth rate is 6.25%~6.74% that the monthly average growth rate of plant, which is monthly average growth rate, is higher than blank control
2 plants of monthly average growth rate 1.72%~3.58%.
A kind of preparation method for microbial-bacterial fertilizer for promoting tangerine orange to grow that the present embodiment refers to, process flow letter
It is single, short processing time.In addition, containing auxin, antibiotic, phenylacetic acid, amber in the metabolite of the streptomyces microflavus used
Growth regulator ingredient necessary to the plant growths such as acid and the basic element of cell division has conversion the soil nitrogen, phosphorus phosphorus, potassium, improves soil
Earth fertility inhibits germ reproduction, and diseases prevention is kept a full stand of seedings, and stimulates cell division, promotes crop take root, germinate, is mature, promoting active principle
The effect of synthesis；And subtilin, polymyxins, the nystatin, brevibacterium generated in bacillus subtilis growth course
Peptide isoreactivity substance, these active materials have apparent inhibiting effect to the pathogenic bacteria of pathogenic bacteria or autogenous infection；Side spore bud
Spore bacillus can solidify several heavy metals, reduce plant in-vivo heavy metal content；Bacillus polymyxa is to bacillary soil-borne disease
Evil-- plant-bacterial-wilt has good control efficiency；Bacillus licheniformis can generate resistant activity material, and have unique
Biology take oxygen mechanism can inhibit the growth and breeding of pathogenic bacteria.
Therefore, a kind of preparation method of microbial-bacterial fertilizer for promoting tangerine orange to grow of the present embodiment is prepared micro-
Bio-bacterial manure is improving soil microstructure, decomposes the soil organism and toxin, while enhancing plant resistance, moreover it is possible to effectively subtract
The generation of few pest and disease damage, reduces the use of pesticide, promotes quality of agricultural product and mouthfeel, and environmentally friendly, soil can be inhibited true
Bacterium growth, diseases prevention keep a full stand of seedings, convert the elements such as nitrogen, phosphorus, potassium in soil, improve the fertility of soil, and irritating plant growth
Effect.
Above embodiment is preferred case of the invention, the protection scope being not intended to limit the invention.
Claims (6)
1. a kind of microbial-bacterial fertilizer for promoting tangerine orange to grow, which is characterized in that including following component:
Wherein, the microbial inoculum is the 0.1% streptomyces microflavus bacterium solution of 5:4:1,0.2% bacillus by mass ratio
Bacterium solution and 0.05% phosphate-solubilizing bacteria bacterium solution are constituted.
2. the microbial-bacterial fertilizer according to claim 1 for promoting tangerine orange to grow, it is characterised in that:
Wherein, the bacillus bacterium solution is the bacillus subtilis of 1:1:1:1, bacillus laterosporus, mostly glutinous bud by mass ratio
Spore bacillus and bacillus licheniformis are formulated.
3. the microbial-bacterial fertilizer according to claim 1 for promoting tangerine orange to grow, it is characterised in that:
Wherein, the auxiliary agent include mass ratio be the 0.8% dibastic sodium phosphate solution of 2:2:1:1,0.4% ammonium sulfate,
0.15% ammonium chloride solution, 0.13% calcium chloride solution.
4. a kind of preparation method of the microbial-bacterial fertilizer for promoting tangerine orange to grow as described in claim 1, which is characterized in that
Include the following steps:
Step 1, by 15 mass parts~25 mass parts microbial inoculum, 20 mass parts~30 mass parts soy meal, 10 mass
Sucrose, the 5 mass parts~10 mass parts auxiliary agent of part~15 mass parts are uniformly mixed, and obtain mixture；
Step 2, the water that 30 times of quality are added in the mixture is dissolved, and is then sent out in 30 DEG C of gnotobasis
Ferment obtains fermentation liquid；
Step 3,2m is passed through in the fermentation liquid3The air of/h/L, and the fermentation liquid is placed in upper, middle and lower and is respectively had there are two leaf
It is stirred in the blender of piece, 18~20h is cultivated under the revolving speed of 60r/min, obtains microbial-bacterial fertilizer.
5. the preparation method of the microbial-bacterial fertilizer according to claim 4 for promoting tangerine orange to grow, it is characterised in that:
Wherein, in the step 1, the preparation of microbial inoculum includes following sub-step:
Step 1-1 takes 200 grams of peeled potatoes, is cut into small pieces, and adds 1000 milliliters of water, boils 30 minutes, filters off potato ball,
Filtrate is then complemented to 1000 milliliters, adds 20 grams of glucose, 15 grams of agar, after dissolution, is fitted into eggplant-shape bottle, is sterilized 30 points
Clock obtains culture medium；
A certain amount of thin yellow chain certain kind of berries bacterium is added in step 1-2 in the medium, in 36 DEG C of at a temperature of 14~18h of culture, obtains
To 0.1% thin yellow chain certain kind of berries bacterium bacterium solution；
Step 1-3, respectively by a certain amount of bacillus subtilis, a certain amount of bacillus laterosporus, a certain amount of more glutinous gemma
Bacillus, a certain amount of bacillus licheniformis are placed in respective nutrient agar, cultivate 14 under the conditions of temperature is 30 DEG C
~18h is prepared and is obtained bacillus subtilis bacterium solution, bacillus laterosporus bacterium solution, mostly glutinous gemma bar that mass concentration is 0.2%
Bacterium bacterium solution, Bacillus licheniformis liquid, then by the bacillus subtilis bacterium solution, the bacillus laterosporus bacterium solution, described
Bacillus polymyxa bacterium solution and the Bacillus licheniformis liquid are mixed according to the mass ratio of 1:1:1:1, obtain 0.2%
Bacillus bacterium solution.
6. the preparation method of the microbial-bacterial fertilizer according to claim 5 for promoting tangerine orange to grow, it is characterised in that:
Wherein, in the step 1-3, the culture medium of the bacillus subtilis is by 10 grams of peptone and 5 grams of yeast
Cream is dissolved in the formation of 1000 milliliters of water,
The culture medium of the bacillus laterosporus is dissolved in by 10 grams of peptone, 3 grams of beef extract and 3 grams of sodium chloride
The formation of 1000 milliliters of water,
The culture medium of the Bacillus polymyxa be by 17.5 grams of soluble starch, 25 grams of peptone and 1.25 gram oxygen
Change sodium is dissolved in the formation of 1000 milliliters of water,
The culture medium of the bacillus licheniformis is by 10 grams of peptone, 15 grams of beef extract and the dissolution of 15 grams of sodium chloride
In the formation of 1000 milliliters of water.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110577437A (en) * | 2019-09-10 | 2019-12-17 | 青岛明月蓝海生物科技有限公司 | liquid seaweed microbial fertilizer and preparation method thereof |
| CN110591964A (en) * | 2019-09-29 | 2019-12-20 | 河南新仰韶生物科技有限公司 | Compound bacterium for improving millennium fruit yield or preventing and treating millennium fruit bacterial wilt and application thereof |
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| CN110577437A (en) * | 2019-09-10 | 2019-12-17 | 青岛明月蓝海生物科技有限公司 | liquid seaweed microbial fertilizer and preparation method thereof |
| CN110591964A (en) * | 2019-09-29 | 2019-12-20 | 河南新仰韶生物科技有限公司 | Compound bacterium for improving millennium fruit yield or preventing and treating millennium fruit bacterial wilt and application thereof |
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