CN103468608A - Complex flora for sewage treatment and culture method of complex flora - Google Patents
Complex flora for sewage treatment and culture method of complex flora Download PDFInfo
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- CN103468608A CN103468608A CN2013103757042A CN201310375704A CN103468608A CN 103468608 A CN103468608 A CN 103468608A CN 2013103757042 A CN2013103757042 A CN 2013103757042A CN 201310375704 A CN201310375704 A CN 201310375704A CN 103468608 A CN103468608 A CN 103468608A
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Abstract
The invention provides a complex flora for sewage treatment and a culture method of the complex flora in order to solve the problem that the sewage treatment cost is increased due to the fact that the complex flora for the sewage treatment is susceptible to survival conditions, is poorer in stability and is required to be supplemented continuously in the prior art. The complex flora for the sewage treatment comprises the following ingredients by weight percentage: 16-25% of photosynthetic flora, 13-20% of lactic acid flora, 12-20% of yeast flora, 5-10% of ray flora, 3-12% of acetobacter flora, 15-26% of desulfuration flora, 1-6% of bacillus flora and 1-6% of bifidobacterium flora. The complex flora for the sewage treatment and the culture method of the sewage treatment have the beneficial technical effects that the complex flora can be fed at one time, the strain depression problem is better solved, the treatment effect is good, and the complex flora is safe to use and the like. The culture method of the complex flora for the sewage treatment is simple to operate and wide in condition, and a survival rate is high.
Description
Technical field
The present invention relates to a kind of technology that adopts composite bacteria to carry out sewage disposal, specially refer to composite bacteria flora and cultural method thereof for a kind of sewage disposal.
Background technology
The composite bacteria flora can suppress the harmful microbe Growth and reproduction owing to having, and has the effects such as protozoon of water-purifying function in the stink that the materials such as inhibition sulfur-bearing, nitrogen produce and activation water, by the increasingly extensive sewage disposal that is applied to.The composite bacteria flora mainly comprises the bacterial classifications such as lactobacillus, yeast flora, Gram positive actinomycetes and bacillus aceticus, and form a powerful function colony, the useful matter that various microorganisms produce in its process of growth and secretory product, become separately or matrix and the raw material of growth each other, go out antioxidant by this mutual promoting action metabolism, generate the stable and complicated ecosystem, thereby utilize the biological synthesis effect of microorganism and waterplant to reach the purpose of purifying liquid waste.The composite bacteria flora has under the synthetic state of fermentation the characteristics of " symbiosis, coexist, common prosperity ", need to survive under given conditions and keep specific bacterial classification ratio, can have better sewage purification function.Described life condition comprises nutrition, pH value, redox point position, ionic strength and culture temperature etc.The prior art sewage disposal with the composite bacteria flora in the process of disposing of sewage, because life condition is not all optimum condition to each bacterial classification of flora, cause the growth velocity of various bacterial classifications to be not quite similar, cause the out of proportion of each bacterial classification in the composite bacteria flora and affect effect.In addition, the less stable of composite bacteria flora for the prior art sewage disposal, easily affected by environment and group structure impact, make some bacterial classification death or get involved and cause the community superiority of composite bacteria flora to be changed because of new microorganism.In addition, due to the loss of composite bacteria flora, needing constantly to supplement the composite bacteria flora in Sewage treatment systems becomes the dominant microflora in system to maintain it, and this can increase the working cost of sewage work undoubtedly.Obviously, the prior art sewage disposal is subject to life condition impact with the composite bacteria flora, less stable and need constantly to supplement the composite bacteria flora, thus cause cost of sewage disposal to add the problems such as high.
Summary of the invention
Be subject to the life condition impact for solving the prior art sewage disposal with the composite bacteria flora, less stable and need constantly to supplement the composite bacteria flora, thus cause cost of sewage disposal to add the problems such as height.The present invention proposes composite bacteria flora and cultural method thereof for a kind of sewage disposal.Sewage disposal of the present invention comprises photosynthetic bacteria group 16% to 25%, lactobacillus 13% to 20%, yeast flora 12% to 20 %, actinomycete group 5% to 10%, bacillus aceticus group 3% to 12%, the desulfurization flora 15% to 26% of weight percent, genus bacillus group 1% to 6% and Bifidobacteria population 1% to 6% with the composite bacteria flora.
Composite bacteria bacteria group culture method for sewage disposal of the present invention comprises the following steps:
S1, add pure water 80～120kg in culture vessel, add brown sugar 4～6kg, stirring and dissolving is made nutrient solution;
S2, add composite bacteria bacterial classification 3.4～6.4kg in the nutrient solution of step S1, wherein, photosynthetic bacteria group bacterial classification 0.7～1.5kg, lactobacillus bacterial classification 0.4～0.8kg, yeast flora bacterial classification 0.7～1.1kg, actinomycete group bacterial classification 0.5～0.9kg, bacillus aceticus group bacterial classification 0.3～0.5 kg, desulfurization flora 0.4kg～1kg, genus bacillus group bacterial classification 0.2～0.3kg and Bifidobacteria population bacterial classification 0.2kg～0.3kg;
S3, urea 0.4～0.6kg, potassium primary phosphate 180～220g and vitamins C 40～60g are dissolved in respectively in 5L water, then join in the nutrient solution of step S2, and shake it is mixed;
S4, sealing culture vessel, and cultivate under illumination, temperature remains on 25～35 ℃;
Shake S5, later two day every day even twice, ventilative half an hour at every turn;
S6, D-araboascorbic acid 50g was dissolved in water in the 3rd day, adds in nutrient solution, shake it is mixed;
S7, within the 4th day, stop illumination, detect pH value, reach below 3.8 and be and cultivate successfully, otherwise, continue cultivation by step S4 and S5, until reaching below 3.8 to be, cultivates successfully the detection pH value;
S8, will cultivate the sealing of successful composite bacteria flora and preserve or come into operation.
Sewage disposal of the present invention is that the composite bacteria flora can adopt dosing method one time with the useful technique effect of composite bacteria flora and cultural method thereof, preferably resolve the decline problem of bacterial classification, through actual use the more than 5 years, do not reached make a variation, treatment effect is good and the safe handling texts.Sewage disposal of the present invention is simple to operate by composite bacteria bacteria group culture method, condition is wide in range and surviving rate is high.
The accompanying drawing explanation
Accompanying drawing 1 is the step schematic diagram of sewage disposal of the present invention by composite bacteria bacteria group culture method.
Below in conjunction with the drawings and specific embodiments, sewage disposal of the present invention is further described with composite bacteria flora and cultural method thereof.
Embodiment
Accompanying drawing 1 is composite bacteria bacteria group culture method steps schematic diagram for sewage disposal of the present invention, as seen from the figure, sewage disposal of the present invention comprises photosynthetic bacteria group 16% to 25%, lactobacillus 13% to 20%, yeast flora 12% to 20 %, actinomycete group 5% to 10%, bacillus aceticus group 3% to 12%, the desulfurization flora 15% to 26% of weight percent, genus bacillus group 1% to 6% and Bifidobacteria population 1% to 6% with the composite bacteria flora.
Composite bacteria bacteria group culture method for sewage disposal of the present invention comprises the following steps:
S1, add pure water 80～120kg in culture vessel, add brown sugar 4～6kg, stirring and dissolving is made nutrient solution;
S2, add composite bacteria bacterial classification 3.4～6.4kg in the nutrient solution of step S1, wherein, photosynthetic bacteria group bacterial classification 0.7～1.5kg, lactobacillus bacterial classification 0.4～0.8kg, yeast flora bacterial classification 0.7～1.1kg, actinomycete group bacterial classification 0.5～0.9kg, bacillus aceticus group bacterial classification 0.3～0.5 kg, desulfurization flora 0.4kg～1kg, genus bacillus group bacterial classification 0.2～0.3kg and Bifidobacteria population bacterial classification 0.2kg～0.3kg;
S3, urea 0.4～0.6kg, potassium primary phosphate 180～220g and vitamins C 40～60g are dissolved in respectively in 5L water, then join in the nutrient solution of step S2, and shake it is mixed;
S4, sealing culture vessel, and cultivate under illumination, temperature remains on 25～35 ℃;
Shake S5, later two day every day even twice, ventilative half an hour at every turn;
S6, D-araboascorbic acid 50g was dissolved in water in the 3rd day, adds in nutrient solution, shake it is mixed;
S7, within the 4th day, stop illumination, detect pH value, reach below 3.8 and be and cultivate successfully, otherwise, continue cultivation by step S4 and S5, until reaching below 3.8 to be, cultivates successfully the detection pH value;
S8, will cultivate the sealing of successful composite bacteria flora and preserve or come into operation.
In culturing process, having suspended substance to produce is normal circumstances, and general temperature high suspended matter easily produces.If it is on the low side to cultivate temperature, the inflammable gas that the composite bacteria flora of cultivation produces will reduce.In addition, it is very normal that peculiar smell is arranged in cultivation, if ventilative two days peculiar smell are also very serious again after qualified for PH, has just likely cultivated unsuccessfully.Owing to there being inflammable gas to produce in culturing process, should be noted fire safety.
Specific embodiment
For the validity of checking sewage disposal of the present invention by composite bacteria bacteria group culture method, the additional proportion of the various floras that employing is described in table 1 below, according to composite bacteria bacteria group culture method for aforementioned sewage disposal of the present invention, obtain cultivating successful sewage disposal and be listed in the table below in 1 with the composite bacteria flora.
Table 1: composite bacteria bacteria group culture statistical effect for sewage disposal
As shown in Table 1, in composite bacteria flora after cultivating, comprise, photosynthetic bacteria group 16% to 25%, lactobacillus 13% to 20%, yeast flora 12% to 20%, actinomycete group 5% to 10%, bacillus aceticus group 3% to 12%, desulfurization flora 15% to 26%, genus bacillus group 1% to 6% and Bifidobacteria population 1% to 6%.And this composite flora is suitable for vitriol, organism and NH
3the sewage that-N content is higher is processed.When having vitriol to exist, in the presence of desulfurization fox bacterium (be in the desulfurization flora a kind of), can with H
2effect, generate H frowzy
2s, and sulphur photosynthetic bacterium (be in photosynthetic bacteria group a kind of) can make H
2s and CO
2effect, generate free sulfur and separate smelly.Therefore, adopt sewage disposal of the present invention to carry out sewage disposal with the composite bacteria flora, often stink is lower, also very favourable to space environment.In double oxygen environment, amphimicrobe (refer to can be under the condition of aerobic and anaerobic the bacterium of metabolism and growth, comprise some bacterium in above-mentioned flora, as yeast) is more active, plays equally the acidication effect, than anaerobically fermenting, better effect is arranged sometimes.Under aerobic condition, organism, NH
3the mechanism of degradation of-N is identical with prior art composite bacteria flora, but remove efficiency ratio prior art composite bacteria flora, wants high.
Following table 2 is for adopting sewage disposal of the present invention to carry out the actual effect statistics of sewage disposal with the composite bacteria flora.
Table 2: composite bacteria flora treatment effect statistics for sewage disposal of the present invention
As can be seen from Table 2, sewage disposal of the present invention uses the composite bacteria flora to vitriol, organism and NH
3the effect that the sewage that-N content is higher is processed is better.Wherein, sulphate content 500～1500g/100ml from processing drops to below 3.3g/100ml, and organism drops to below 0.3g/100ml from 500～1500g/100ml, NH
3-N drops to below 0.15g/100ml from 100～500g/100ml, and its treatment effect is fairly obvious.Sewage after processing has reached national relevant emission standards, can directly discharge fully, or be recycled.In addition, adopt sewage disposal of the present invention to carry out sewage disposal with the composite bacteria flora, its COD content is higher, is suitable for anaerobically fermenting, after being equipped with corresponding facility, when reducing pollutent, also obtains obtaining renewable energy resources biogas.Have again, while adopting sewage disposal of the present invention to carry out sewage disposal with the composite bacteria flora, not there will be the phenomenon of flora decline.Do not need to throw in a large number composite floras again in sewage.In actual use, the composite flora release time the earliest surpasses 5 years, the phenomenons such as flora decline, variation, the obvious reduction for the treatment of effect do not occur yet.
Obviously, sewage disposal of the present invention is that the composite bacteria flora can adopt dosing method one time with the useful technique effect of composite bacteria flora and cultural method thereof, preferably resolve the decline problem of flora, through actual use the more than 5 years, do not reached make a variation, treatment effect is good and the safe handling texts.Sewage disposal of the present invention is simple to operate by composite bacteria bacteria group culture method, condition is wide in range and surviving rate is high.
Claims (2)
1. a sewage disposal composite bacteria flora, it is characterized in that, this composite bacteria flora comprises photosynthetic bacteria group 16% to 25%, lactobacillus 13% to 20%, yeast flora 12% to 20 %, actinomycete group 5% to 10%, bacillus aceticus group 3% to 12%, the desulfurization flora 15% to 26% of weight percent, genus bacillus group 1% to 6% and Bifidobacteria population 1% to 6%.
2. a composite bacteria bacteria group culture method for the described sewage disposal of claim 1, is characterized in that, the method comprises the following steps:
S1, add pure water 80～120kg in culture vessel, add brown sugar 4～6kg, stirring and dissolving is made nutrient solution;
S2, add composite bacteria bacterial classification 3.4～6.4kg in the nutrient solution of step S1, wherein, photosynthetic bacteria group bacterial classification 0.7～1.5kg, lactobacillus bacterial classification 0.4～0.8kg, yeast flora bacterial classification 0.7～1.1kg, actinomycete group bacterial classification 0.5～0.9kg, bacillus aceticus group bacterial classification 0.3～0.5 kg, desulfurization flora 0.4kg～1kg, genus bacillus group bacterial classification 0.2～0.3kg and Bifidobacteria population bacterial classification 0.2kg～0.3kg;
S3, urea 0.4～0.6kg, potassium primary phosphate 180～220g and vitamins C 40～60g are dissolved in respectively in 5L water, then join in the nutrient solution of step S2, and shake it is mixed;
S4, sealing culture vessel, and cultivate under illumination, temperature remains on 25～35 ℃;
Shake S5, later two day every day even twice, ventilative half an hour at every turn;
S6, D-araboascorbic acid 50g was dissolved in water in the 3rd day, adds in nutrient solution, shake it is mixed;
S7, within the 4th day, stop illumination, detect pH value, reach below 3.8 and be and cultivate successfully, otherwise, continue cultivation by step S4 and S5, until reaching below 3.8 to be, cultivates successfully the detection pH value;
S8, will cultivate the sealing of successful composite bacteria flora and preserve or come into operation.
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Cited By (6)
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| CN106238456A (en) * | 2016-09-21 | 2016-12-21 | 成都创慧科达科技有限公司 | A kind of microbial composite bacteria substrate of rehabilitating soil and application thereof |
| CN107164264A (en) * | 2017-06-02 | 2017-09-15 | 吴万波 | Multiple-effect composite bacteria agent and application thereof |
| CN107267409A (en) * | 2017-04-27 | 2017-10-20 | 浙江九田环保科技有限公司 | A kind of preparation method of environmental protection compound bacteria flora |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN113151141A (en) * | 2021-06-01 | 2021-07-23 | 福建傲农生物科技集团股份有限公司 | Culture method and culture medium of strain for sewage treatment |
| CN114874936A (en) * | 2022-04-28 | 2022-08-09 | 北京环营生物环保科技有限公司 | Composite microbial inoculum for domestic sludge treatment, preparation method and application thereof |
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| CN114874936A (en) * | 2022-04-28 | 2022-08-09 | 北京环营生物环保科技有限公司 | Composite microbial inoculum for domestic sludge treatment, preparation method and application thereof |
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Application publication date: 20131225 |