CN102492640A

CN102492640A - Aeromonas capable of removing hydrogen sulfide (H2S) gas in gas and usage thereof

Info

Publication number: CN102492640A
Application number: CN201110415793XA
Authority: CN
Inventors: 田青; 姜秀光; 郝赟; 陈爱因
Original assignee: Water Treatment New Technology Industrialization Base Of Ministry Of Corporation(tianjin Free Trade Zone Water Treatment New Technology Industrialization Base)
Current assignee: CAPITAL AIHUA (TIANJIN) MUNICIPAL & ENVIRONMENTAL ENGINEERING Co.,Ltd.
Priority date: 2011-12-14
Filing date: 2011-12-14
Publication date: 2012-06-13
Anticipated expiration: 2031-12-14
Also published as: CN102492640B

Abstract

The invention discloses aeromonas capable of removing hydrogen sulfide (H2S) gas in a gas and usage thereof, wherein aeromonas sp. CGMCC No.5279 is capable of removing H2S gas in gas. Experimental results show that: by placing the (Aeromonas sp.) CGMCC No.5279 disclosed by the invention on a ceramic grain filter and then filling the same on a bio-trickling filter, the bio-trickling filter can remove H2S gas rapidly, and after the treatment, the exit mass concentration of the H2S is less than 0.03 mg/m<3> and reaches the factory primary standard in the GB14554-93 Emission Standards for Odor Pollutants.

Description

H in a kind of removal gas 2The Aeromonas of S gas and purposes

Technical field

The present invention relates to a strain and remove H in the gas ₂The Aeromonas of S gas and purposes.

Background technology

H ₂S has pungency and asphyxiant colourless gas, and lower concentration just has the intensive foul odour.Sucking H ₂When S concentration is low, can see significantly hormesis, when inhaled concentration was higher, olifactory nerve tip paralysis can be to H ₂The S stink disappears to continue and then goes into a coma, even dead.

The treatment process of tradition hydrogen sulfide not only costs an arm and a leg but also is prone to cause secondary pollution, facts have proved biological method purification atmospheric pollution Cheap highly effective.Wherein bio-trickling method purification odor pollution gas is a newer Environmental Biotechnology, also is the most promising at present waste gas biologic treating technique.

When process or behind pretreated waste gas entering bio-trickling Tower System; Through when the biomembranous packing layer; After repugnant substance in the gas is water-soluble, is recycled liquid again and, reaches the purpose of Purge gas attached to the absorption of the mikrobe on the filling surface, absorption, degraded.

Existing hydrogen sulfide biologic treating technique is often with on the active sludge direct inoculation filler, and the formation microbial film, the effective microbe ratio is low in the film, also need behind the extension film carry out the domestication of long period to it, and operational condition is controlled than difficult in the domestication process.Therefore need badly a kind of can be at bio-trickling filter rapidly and efficiently to H ₂The bacterial strain that S gas is handled.

Summary of the invention

The objective of the invention is to overcome deficiency of the prior art, provide a kind of and can remove H in the gas efficiently ₂The Aeromonas of S gas.

Second purpose of the present invention provides a kind ofly can remove H in the gas efficiently ₂The purposes of the Aeromonas of S gas.

Technical scheme of the present invention is summarized as follows:

H in a kind of removal gas ₂The Aeromonas of S gas (Aeromonas sp.) CGMCC No.5279, it has H in the gas of removal ₂The ability of S gas.

H in a kind of removal gas ₂The Aeromonas of S gas (Aeromonas sp.) CGMCC No.5279 removes H in the gas ₂The purposes of S gas.

Experimental result shows: after filling in bio-trickling filter after being arranged on Aeromonas of the present invention (Aeromonas sp.) CGMCC No.5279 on the ceramic grain filter, it can make bio-trickling filter can remove H fast ₂S gas is handled back H ₂S outgoing quality concentration is less than 0.03mg/m ³, reach the factory's circle primary standard among the GB14554-93 " odorant pollutant emission standard ".

Description of drawings

Fig. 1 handles for bio-trickling filter and contains H ₂The experimental installation synoptic diagram of S gas.

Fig. 2 handles for bio-trickling filter and contains H ₂The equipment synoptic diagram of S gas.

Embodiment

Through specific embodiment the present invention is further described below.

Embodiment 1

Remove H in the gas ₂The isolation cultivation method of the Aeromonas Aeromonas sp. of S gas comprises the steps:

(1) mud is taken from Jizhuangzi Waste Water Treatment Plant, Tianjin, and supplement the nutrients every day liquid and aeration reach more than the 90mL/g SVI, accomplishes microorganism culturing.Stop to add nutritive medium, add sodium thiosulfate solution (sulfonium ion concentration is 2g/L) every day, keep ventilation, after 15 days wherein desulfurization bacterium become dominant bacteria, the desulfurization bacterium domestication is accomplished.

Nutritive medium is formed: glucose 0.1g, peptone 0.5g, K ₂HPO ₄0.6g, KH ₂PO ₄0.6g, MgCl ₂6H ₂O0.1g, NH ₄Cl 0.2g, small amount of Fe SO ₄7H ₂O and MgSO ₄-5H ₂O, adding distil water is to 1000mL.

(2) muddy water mixed solution that step (1) is obtained takes out 10mL, adds in the Erlenmeyer flask that 90mL sterile purified water and 20 sterilization granulated glass spherees have been housed, and the 20min that fully vibrates smashes the mud flco, makes bacterial cell fully be discharged in the supernatant; Leave standstill 30min, draw the adding of 1mL supernatant with aseptic straw and be equipped with in the test tube of 9mL sterile distilled water, be mixed with 10 ^-2The solution of concentration, by that analogy, preparation 10 ^-3～10 ^-7The solution of concentration;

(3) choosing gradient is 10 ^-5～10 ^-7Bacteria suspension, get 1mL respectively and be inoculated in the substratum, each extent of dilution is done 3 parallel appearance, numbering; Under the condition of aerobic, preserve moisture for 10 ℃ and cultivated for 3 weeks;

(4) choosing colony is big in the culture medium flat plate from step (3); Single bacterium colony that form is different, line separates once more in the solid medium flat board, repeats plate streaking and separates; Make the homomorphosis of bacterium colony in microscope on the flat board; Single, similar single bacterium colony, the called after Aeromonas Aeromonas sp. of being of size

Solid medium: Na ₂S ₂O ₃5H ₂O 8g, K ₂HPO ₄2.0g, KH ₂PO ₄2.0g, NH ₄Cl 0.4g, MgCl ₂6H ₂O 0.2g, FeSO ₄7H ₂O 0.01g, agar 20g, adding distil water are to 1000mL, and transferring pH is 7.0.

Embodiment 2

Aeromonas Aeromonas sp. of the present invention has following Microbiological Characteristics:

1. morphological characteristic

Examine under a microscope, for shaft-like, long 2.0 μ m, wide 0.62 μ m; On substratum, form the oyster white bacterium colony.

2. cultivate and learn characteristic

Under 10 ℃, when in solid medium, cultivating, (Aeromonas sp.) of the present invention bacterial strain has following characteristic.

Smooth surface is moistening, rat, and the edge indentation, thalline is rounded, and color is a beige.

3. physiological property

Gram negative bacterium, catalase is negative, methyl red test is positive, hydrolyzed starch, liquefy gelatin, edwardsiella hoshinae, growth pH value are 6～8,5～30 ℃ of growth temperatures.

Learn characteristic according to mentioned microorganism; Called after Aeromonas (Aeromonas sp.); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 22nd, 2011, deposit number is CGMCC No.5279, and the preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, and survived.

Embodiment 3

Aeromonas (Aeromonas sp.) is to gas H ₂The removal effect of S gas.

With the Aeromonas that filters out (Aeromonas sp.), be inoculated into and carry out enlarged culturing in the nutrient solution, form bacteria suspension; Ceramic grain filter is soaked wherein, add nutrient solution every day and continue aeration, filter material surface microbial film thickness reaches 1～2m, hangs film and accomplishes; The filtrate that hangs up properly film is successively added in the bio-trickling filter (see figure 1), at spray liquid flow 8L/h, contain H ₂The air flow quantity 0.4m of S ³/ h, H wherein ₂S mass concentration 0.2～0.25mg/m ³, room temperature processing condition under starter gear.Treat H ₂The clearance of S has reached more than 85%, and outgoing quality concentration is controlled at 0.03mg/m ³Below, show that the bio-trickling tower apparatus has started to finish, get into steady operational status.

After stable, move 40 days more continuously under the same processing condition, install stable continuously, H ₂The clearance of S is stabilized in more than 85%, and it is very good to move treatment effect continuously, reaches the factory's circle primary standard among the GB14554-93 " odorant pollutant emission standard ".

With comparing on Aeromonas of the present invention (Aeromonas sp.) the inoculation filler with on the active sludge direct inoculation filler; The filtrate that has Aeromonas of the present invention (Aeromonas sp.) is positioned in the bio-trickling filter; Removal hydrogen sulfide that can be stable after 5 days, and the bio-trickling filter of common inoculation of activated-sludge could stable removal hydrogen sulfide after general 2 weeks.

The composition of nutrient solution is: Na ₂S ₂O ₃5H ₂O 8g, K ₂HPO ₄2.0g, KH ₂PO ₄2.0g, NH ₄CI 0.4g, MgCl ₂6H ₂O 0.2g, FeSO ₄7H ₂O 0.01g, adding distil water are to 1000mL, and transferring pH is 7.0.

Claims

1. remove H in the gas for one kind ₂The Aeromonas of S gas (Aeromonas sp.) CGMCC No.5279, it has H in the gas of removal ₂The ability of S gas.

2. H in a kind of removal gas of claim ₂The Aeromonas of S gas (Aeromonas sp.) CGMCC No.5279 removes H in the gas ₂The purposes of S gas.