CN106582258A - Method for removing hydrogen sulfide by utilizing heterotrophic microorganisms - Google Patents
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Abstract
The invention discloses a method for removing hydrogen sulfide by utilizing heterotrophic microorganisms. Specific heterotrophic microorganisms are added in a device for removing hydrogen sulfide, an aerobic reaction is performed in a set reaction system, and removal on H2S, HS- or/and S2- in the reaction system of the device is implemented, wherein the heterotrophic microorganisms mean a category of heterotrophic bacteria of which a genome comprises a sulfide:quinone oxidoreductase gene and a persulfid dioxygenase gene and which can oxidize sulfides to generate zero-valent sulphur and thiosulfate, or sulfate. According to the method disclosed by the invention, the screened heterotrophic bacteria have efficient capacity of oxidize exogenous hydrogen sulfide, has the characteristics of high growth speed, low culture cost and capacity of forming great biomass, effectively solves the problem of condition limitation in the prior art in which autotrophic bacteria are used for remove hydrogen sulfide, and has a great application potential in the industrial production.
Description
Technical field
The present invention relates to a kind of method of biological removal sulfide, more particularly to one kind gone using heterotrophic microorganism it is vulcanisation
Thing (H2S、HS-Or/and S2-) method.
Background technology
Hydrogen sulfide is the toxic gas of irritant abnormal smells from the patient, be in industrial processes (such as sewage treatment plant, animal husbandry with
And composting plant etc.) the primary waste gases composition that is widely present.At present, the minimizing technology of hydrogen sulfide harmful gass is broadly divided into physics
Method, chemical method and bioanalysises, wherein biological method remove hydrogen sulfide has irreplaceable excellent compared to chemical method and Physical
Gesture, application prospect is relatively broad.
The microorganism during biological removal hydrogen sulfide plays vital effect to the oxidative absorption of hydrogen sulfide.Mesh
The front inoculation method for microorganism in aerobic hydrogen sulfide biological removal reactor mainly has training and artificial inoculation method.Tame and docile
Foster method is referred in the microorganism life naturally being passed through before the gas containing hydrogen sulfide in first adding nutritional solution to make device in a device
Long, then passing to the gas containing hydrogen sulfide makes the microorganism that can partly play hydrogen sulfide removal effect work, by such as
This is tamed for a long time, and the microbes that can remove hydrogen sulfide are enriched with, and can not remove the microbes quilt of hydrogen sulfide
Eliminate, it can be seen that selection of this method to microorganism has blindness.Artificial inoculation method is divided to two kinds again, and one kind is inoculation tool
There is the activated sludge of hydrogen sulfide removal ability, this method actually has same blindness with above-mentioned training；It is another kind of
It is that inoculation can remove the pure culture microorganism of hydrogen sulfide, refer mainly to early discovery can be by the use of hydrogen sulfide as electron donor
Chemautotrophy and photoautotrophic microorganism of the energy for own growth is generated, that is, is commonly referred to as Thiobacillus
(Thiobacillus), solfataricus genus (Sulfolobus), or green sulphur bacteria (Chlorobium).However, such autotrophy is thin
Bacterium is compared to heterotrophic bacteria poor growth, it is difficult to form larger Biomass, and its growth generally needs the environment of slant acidity, and
Acid condition is but unfavorable for the absorption and oxidation of hydrogen sulfide.Jing is retrieved, and is gone down in aerobic conditions using heterotrophic microorganism vulcanisation
The method of hydrogen yet there are no reports for work.
The content of the invention
Blindness sex chromosome mosaicism and autotrophic bacteria for training in prior art removes the limitation of hydrogen sulfide application, this
Bright purpose is to provide a kind of using heterotrophic microorganism removal hydrogen sulfide (H2S、HS-Or/and S2-) method.
The method that utilization heterotrophic microorganism of the present invention removes sulphide removal, is that life is added in the device for go sulphide removal
The heterotrophic microorganism of long stable phase, under setting reaction system aerobic reaction is carried out, and is realized to the H in device reaction system2S、
HS-Or/and S2-Remove；
It is characterized in that：
The heterotrophic microorganism refers to and contain in its genome thioquinones oxidoreductase (sulfide:quinone
Oxidoreductase, sqr) gene and sulfur dioxygenase (persulfid dioxygenase, pdo) gene a class heterotrophism
Microorganism；
The reaction system is：Concentration is 4- (2- the ethoxys) -1- piperazine ethanesulfonic acid (HEPES of 50 ± 5mM:4-(2-
Hydroxyethyl) -1-piperazineethanesulfonic acid) buffer, or have buffer capacity at neutral ph
Power and the buffer harmless to the heterotrophic microorganism；Also added with the sulfur that concentration is 10 μM～1000 μM wherein in the reaction system
Change the heterotrophic microorganism bacterial turbidity OD contained in sodium, and the reaction system600nm=0.5～10；
The aerobic reaction condition is：10 DEG C～40 DEG C of temperature, pH 6～9,200 ± 50r.p.m. concussion；
H in described device reaction system2S、HS-Or/and S2-After removal, according to the difference of heterotrophic microorganism bacterial strain used
Its product is sulfur gastral cavity sulfur and thiosulfate, or sulfate.I.e.：It is will to change into sulfur after Oxidation of Hydrogen Sulfide using heterotrophic microorganism
Thiosulfate and sulfur gastral cavity sulfur, or completely it is oxidized to sulfate.
Above-mentioned utilization heterotrophic microorganism is removed in the method for hydrogen sulfide：The heterotrophic microorganism is preferably Gluconobacter oxydans
Bacillus (Gluconobacter oxydans) 621H, pseudomonasputida (Pseudomonas putida) S16, Aerugo are false single
Born of the same parents bacterium (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium (Cupriavidus necator) JMP134 or ocean
Herba Alii fistulosi burkholderia (Burkholderia cepacia) ATCC 25416.
Further, the cultural method of the heterotrophic microorganism is：30 ± 2 DEG C in defined medium, 200 ±
10r.p.m. concussion and cultivates reach stable phase in 24 ± 4 hours to the bacterial growth cycle；
Wherein：The defined medium formula is：Industrial fermentation glucose 30g/L, industrial fermentation Dried Corn Steep Liquor Powder
15g/L。
Above-mentioned utilization heterotrophic microorganism is removed in the method for hydrogen sulfide, and the reaction system is preferably：Concentration is 50mM's
4- (2- ethoxys) -1- piperazine ethanesulfonic acid buffer, also added with the sulfuration that concentration is 20 μM～800 μM wherein in the reaction system
The heterotrophic microorganism bacterial turbidity OD contained in sodium, and the reaction system600nm=1～8.
Above-mentioned utilization heterotrophic microorganism is removed in the method for hydrogen sulfide, and the aerobic reaction condition is preferably：20 DEG C of temperature
～37 DEG C, pH 7～8,120 ± 20r.p.m. concussion.
Above-mentioned utilization heterotrophic microorganism is removed in the method for hydrogen sulfide, the pseudomonasputida (Pseudomonas
Putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium (Cupriavidus
Necator) JMP134 or Burkholderia cepacia (Burkholderia cepacia) bacterial strains of ATCC 25416 are aerobic anti-
The ability of oxidation of sulfureted hydrogen in system is answered to strengthen as external source adds the induction of hydrogen sulfide, polysulfide or elemental sulfur.
Present invention applicant is found that a series of heterotrophic microorganism containing Oxidation of Hydrogen Sulfide system can be efficiently rapid
Oxidation external source hydrogen sulfide, different depending on antibacterial, its product is sulfur gastral cavity sulfur and thiosulfate or for sulfate.Experiment confirms this
A bit heterotrophic microorganisms have growth rapid compared to autotrophic microbe, and Biomass is big, cultivate the characteristics of facilitating economic.And its unit
Dry weight oxidation rate is suitable with the autotrophic bacteria sulfur oxide compound speed reported before.Based on this, the invention provides a kind of profit
The method for removing hydrogen sulfide with heterotrophic microorganism, the method solves well the deficiency of hydrogen sulfide removal in prior art,
There is very big application potential in commercial production.
To sum up, the present invention has the advantage that compared with prior art：
1. the present invention is have according to the heterotrophic bacteria that bacterial genomes information searching contains oxidation of sulfureted hydrogen key gene
The screening process of purpose, the heterotrophic bacteria for filtering out has the ability for efficiently aoxidizing very much external source hydrogen sulfide, compared to reporting
Autotrophic bacteria [the Immobilized-Cell and Sulfur-Settling Free-Cell Recycle in road
Reactors.Biotechnology Progress,1991.7(6):P.495-500.], wherein Gluconobacter oxvdans
(G.oxydans) oxidability of 621H is higher, and other bacterium oxidabilities are therewith quite (Fig. 2).
2. a series of heterotrophic bacterias that the present invention is filtered out can be according to the oxidation product of the different control sulfide of bacterial strain
For thiosulfate and sulfur gastral cavity sulfur, or for sulfate.
3. the ability of the present invention is filtered out heterotrophic bacteria its oxidation of sulfureted hydrogen has inductive effect.I.e. when external source has sulfur
When changing hydrogen, more expression is strengthened the ability of its sulfur oxide compound to induce its oxidation of sulfureted hydrogen related gene.
4. heterotrophic bacteria growth is rapid, and culture is convenient, and toxigenic capacity is low.
5. the antibacterial that the present invention is filtered out has at a relatively high toleration, when external source concentration of hydrogen sulfide reaches 1mM, still
Can efficiently remove.
Description of the drawings
Fig. 1. Gluconobacter oxvdans (Gluconobacter oxydans) 621H, pseudomonasputida
(Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium
(Cupriavidus pinatubonensis) JMP134 or Burkholderia cepacia (Burkholderia cepacia)
25,416 5 plants of antibacterials of ATCC have significantly oxidation external source hydrogen sulfide ability.
Fig. 2. Gluconobacter oxvdans (Gluconobacter oxydans) 621H, pseudomonasputida
(Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium
(Cupriavidus pinatubonensis) JMP134 or Burkholderia cepacia (Burkholderia cepacia)
25,416 5 plants of bacterial oxidation external source hydrogen sulfide speed of ATCC.(per cell dry weight, oxidized per unit time external source hydrogen sulfide
Amount.)
Fig. 3. hydrogen sulfide, polysulfide and elemental sulfur are to Ralstonia bacterium (Cupriavidus pinatubonensis)
JMP134 (A), Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1 (B), Burkholderia cepacia
(Burkholderia cepacia) ATCC 2541 (C) or pseudomonasputida (Pseudomonas putida) S16 (D) oxygen
Change the inductive effect of hydrogen sulfide.
Fig. 4. Gluconobacter oxvdans (Gluconobacter oxydans) 621H, pseudomonasputida
(Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium
(Cupriavidus pinatubonensis) JMP134 or Burkholderia cepacia (Burkholderia cepacia)
The end-product of 25,416 5 plants of bacterial oxidation hydrogen sulfide of ATCC.
Specific embodiment
Specific enforcement example of the invention is presented herein below.It is pointed out that these embodiments are only exemplary, not
Any restriction is constituted to the scope of the present invention.The details and form of embodiment are carried out under the thinking and scope of the present invention
Modification and replacement are each fallen within protection scope of the present invention.
Gluconobacter oxvdans (Gluconobacter oxydans) 621H, pseudomonasputida (Pseudomonas
Putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1 or Burkholderia cepacia
(Burkholderia cepacia) ATCC 25416 is purchased from American Type Culture Collection center (American Type
Culture Collection), Ralstonia bacterium (Cupriavidus pinatubonensis) JMP134 purchased from it is logical send (on
Sea) bio tech ltd.
The culture medium prescription of heterotrophic microorganism：Industrial fermentation glucose, 30g/L；Industrial fermentation Dried Corn Steep Liquor Powder,
15g/L。
HEPES buffer solution is consisted of (1 liter of distilled water):4- (2- ethoxys) -1- piperazine ethanesulfonic acid (HEPES:4-(2-
Hydroxyethyl) -1-piperazineethanesulfonic acid) 13g, diethylene triamine pentacetic acid (DTPA) (DTPA：
Diethylenetriaminepentaacetic acid) 0.02g, NaOH regulation pH to 7.4.
The detection method of hydrogen sulfide is：1ml samples are taken, the double ammonia reagents of 80ul mixing are added, is mixed and is placed after room temperature
20min, measures OD670nmUnder absorbance, if absorbance is more than 1, will sample deionized water dilute after measure again.Mixing is double
The formula of ammonia reagent such as table 1 below：
Table 1：Two kinds of different formula are selected according to the predicted concentration of hydrogen sulfide.
The preparation of polysulfide：13mg sulphur powders and 70mg sodium sulfide are added in advance with the distilled water of argon deoxygenation, close
Envelope, pH value hydrochloric acid is adjusted to 9.3.
Acetone magister of sulfur：Excessive sulphur powder is added in acetone, concussion fully dissolving, saturated solution is containing about 20mM sulfur.
The detection method of sulfur gastral cavity sulfur is：Plus the boric acid of 0.55ml 1% heats 1min into 1.5ml EP pipes in boiling water, plus
Enter 0.25ml samples, add and cooled down under 0.2ml 0.1M potassium cyanide heating 1min. room temperatures, add 0.1ml nitric acid ferrons, at once
Mixing, if cell, then centrifugation is removed, and measures OD460nm, blank adopts deionized water.
Sulphite, sulfate and thiosulfate adopt chromatography of ions (ICS-1100system；Dionex) detect, tool
Concrete conditions in the establishment of a specific crime is：Flow rate of mobile phase:1ml/min, column temperature:30 DEG C, suppressor type:ASRS_4mm, suppressor electric current:55mA, pressure
Power scope:The appearance time of 200-3000psi.KOH=20mM, sulphite, sulfate and thiosulfate is respectively 7.4 points
Clock, 7.9 minutes, 25.7 minutes.
Embodiment 1：Five plants of heterotrophic bacteria oxidation of sulfureted hydrogen speed researchs.
By five plants of heterotrophic bacterias (Gluconobacter oxvdans (Gluconobacter oxydans) 621H, stench vacation monospore
Bacterium (Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia
Bacterium (Cupriavidus necator) JMP134 or Burkholderia cepacia (Burkholderia cepacia) ATCC
25416) in 30 degrees Celsius of 200rpm concussion and cultivates culture more than 20 hours, bacterial turbidity is more than OD600nmWhen=4, it is collected by centrifugation
Cell, and clean one time with pure water, then by the heterotrophic bacteria cell handled well be dissolved in pH=7.4, containing 50 μM of divinyls
Pentaacetic acid (DTPA:Diethylenetriaminepentaacetic acid) 4- (2- ethoxys) -1- piperazine second sulphurs
Acid (HEPES:4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) in buffer, and adjust thin
Bacterium turbidity is OD600=2.Using when, also cultured bacterium solution can be used for directly or after dilution processing sulfide.
Heterotrophic bacteria cell suspension after 10mL is adjusted is added in 50mL centrifuge tubes, adds freshly prepared end dense immediately
Spend for 200 μM of sodium sulfide initial actions, whole reaction condition is 30 DEG C of slight concussions (100 ± 10r.p.m.), and at 10 minutes,
30 minutes, the residual concentration of hydrogen sulfide in 60 minutes three point in time sampling measure systems.
Recentrifuge collects cell after the completion of reaction, cleans three times in pure water, removes supernatant, is then transferred to freezing dry
Lyophilization more than 12 hours in dry machine, determine dry cell weight.Calculated by the pace of change and dry cell weight of concentration of hydrogen sulfide
Go out every plant of antibacterial per cell dry weight, the unit interval aoxidizes the speed of external source hydrogen sulfide.
Jing above method detects that the above-mentioned five plants heterotrophic bacterias with Oxidation of Hydrogen Sulfide system are respectively provided with significantly efficient oxygen
The ability of outside the pale of civilization source hydrogen sulfide, refers to Fig. 1.
It is computed, they aoxidize the speed of external source hydrogen sulfide in 6 to 50 μm of olmin-1G (dry cell weight)-1Between,
Refer to Fig. 2.
Embodiment 2：The inductive effect research of hydrogen sulfide.
By Ralstonia bacterium (Cupriavidus pinatubonensis) JMP134, Pseudomonas aeruginosa
(Pseudomonas aeruginosa) PAO1, Burkholderia cepacia (Burkholderia cepacia) ATCC 2541,
Or pseudomonasputida (Pseudomonas putida) S16 bacterium by method culture in embodiment 1 to logarithmic growth early stage when
(OD600=0.5), final concentration of 20 μM of Na are additionally added in the medium2S, polysulfide or acetone sulfur, continue to cultivate induction
1 hour, then by method collects thalline in embodiment 1, determine Oxidation of Hydrogen Sulfide speed, and with without induction matched group ratio
Compared with, as a result show hydrogen sulfide, polysulfide and acetone sulfur can Induction of bacterial and strengthen the ability of its oxidation of sulfureted hydrogen,
Refer to Fig. 3.
Embodiment 3：Oxidation of Hydrogen Sulfide Study on product.
According to the condition in embodiment 1, by five plants of heterotrophic bacteria (Gluconobacter oxvdans (Gluconobacter
Oxydans) 621H, pseudomonasputida (Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas
Aeruginosa) PAO1, Ralstonia bacterium (Cupriavidus necator) JMP134 or Burkholderia cepacia
(Burkholderia cepacia) ATCC 25416) cultivate more than 20 hours, when bacterial turbidity is more than OD600When=4, centrifugation
Collect cell, and clean one time with pure water, then by cell after process be dissolved in pH=7.4, containing 50 μM of diethylenetriamines
Pentaacetic acid (DTPA:Diethylenetriaminepentaacetic acid) 4- (2- ethoxys) -1- piperazine ethanesulfonic acids
(HEPES:4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) in buffer, and adjust antibacterial
Turbidity OD600=8.
Heterotrophic bacteria cell suspension after 10mL is adjusted is added in 50mL centrifuge tubes, adds freshly prepared end dense immediately
Spend for 800 μM of sodium sulfide initial actions, whole reaction condition is 30 DEG C of slight concussions (100r.p.m.), and at 30 minutes, 60 points
Clock, the residual concentration of hydrogen sulfide in 120 minutes three point in time sampling measure systems, while testing sulfur gastral cavity sulfur, sulphite, sulfur
The production of hydrochlorate and thiosulfate.
As a result C.necator JMP134 are displayed in six hours by Oxidation of Hydrogen Sulfide into sulfate, and stench vacation monospore
Bacterium (P.putida) S16, Pseudomonas aeruginosa (P.aeruginosa) PAO1, Burkholderia cepacia (B.cepacia)
The final product of ATCC 25416 and Gluconobacter oxvdans (G.oxydans) 621H oxidation of sulfureted hydrogen is sulfur gastral cavity sulfur and thio
Sulfate (see Fig. 4).
Claims (6)
1. a kind of method that utilization heterotrophic microorganism removes sulphide removal, is that growth stable phase is added in the device for go sulphide removal
Heterotrophic microorganism, carry out aerobic reaction under setting reaction system, realize to the H in device reaction system2S、HS-Or/and
S2-Remove；
It is characterized in that：
The heterotrophic microorganism refers to and contain in its genome thioquinones oxidoreductase (sulfide:quinone
Oxidoreductase, sqr) gene and sulfur dioxygenase (persulfid dioxygenase, pdo) gene a class heterotrophism
Microorganism；
The reaction system is：Concentration is 4- (2- the ethoxys) -1- piperazine ethanesulfonic acid buffer of 50 ± 5mM, or in neutrality
There are buffer capacity and the buffer harmless to the heterotrophic microorganism under pH value；Wherein in the reaction system also added with concentration be 10
μM～1000 μM of sodium sulfide, and the heterotrophic microorganism bacterial turbidity OD contained in the reaction system600nm=0.5～10；
The aerobic reaction condition is：10 DEG C～40 DEG C of temperature, pH 6～9,200 ± 50r.p.m. concussion；
H in described device reaction system2S、HS-Or/and S2-After removal, according to its product of the difference of heterotrophic microorganism bacterial strain used
Thing is sulfur gastral cavity sulfur and thiosulfate, or sulfate.
2. the method for removing hydrogen sulfide using heterotrophic microorganism according to claim 1, it is characterised in that：The micro- life of the heterotrophism
Thing refers to Gluconobacter oxvdans (Gluconobacter oxydans) 621H, pseudomonasputida (Pseudomonas
Putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Ralstonia bacterium (Cupriavidus
Necator) JMP134 or Burkholderia cepacia (Burkholderia cepacia) ATCC25416.
3. the method for removing hydrogen sulfide using heterotrophic microorganism according to claim 2, it is characterised in that：The micro- life of the heterotrophism
The cultural method of thing is：30 ± 2 DEG C in defined medium, 200 ± 10r.p.m. concussion and cultivates 24 ± 4 hours are to bacterial growth
Cycle reaches stable phase；
Wherein：The defined medium formula is：Industrial fermentation glucose 30g/L, industrial fermentation Dried Corn Steep Liquor Powder 15g/
L。
4. the method for removing hydrogen sulfide using heterotrophic microorganism according to claim 1, it is characterised in that：The reaction system
It is：Concentration for 50mM 4- (2- ethoxys) -1- piperazine ethanesulfonic acid buffer, wherein in the reaction system also added with concentration be 20
μM～800 μM of sodium sulfide, and the heterotrophic microorganism bacterial turbidity OD contained in the reaction system600nm=1～8.
5. the method for removing hydrogen sulfide using heterotrophic microorganism according to claim 1, it is characterised in that：The aerobic reaction
Condition is：20 DEG C～37 DEG C of temperature, pH 7～8,200 ± 20r.p.m. concussion.
6. the method for removing hydrogen sulfide using heterotrophic microorganism according to claim 1, it is characterised in that：The stench is false single
Spore bacterium (Pseudomonas putida) S16, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Rolls lead to
Salmonella (Cupriavidus necator) JMP134 or Burkholderia cepacia (Burkholderia cepacia) ATCC
The ability of 25416 bacterial strains oxidation of sulfureted hydrogen in aerobic reaction system is with external source addition hydrogen sulfide, polysulfide or elemental sulfur
Induction and strengthen.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218421A (en) * | 1996-05-10 | 1999-06-02 | 帕克斯生物系统公司 | Purification of gases containing hydrogen sulphide |
| CN102321548A (en) * | 2011-08-01 | 2012-01-18 | 浙江工业大学 | Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide |
| CN102424804A (en) * | 2011-12-14 | 2012-04-25 | 青岛思普润水处理有限公司 | Citrobacter sp. for removing H2S gas from gas, and use thereof |
| CN102438443A (en) * | 2009-03-20 | 2012-05-02 | 藻类科学公司 | System and method for treating wastewater via phototactic heterotrophic microorganism growth |
| CN102492640A (en) * | 2011-12-14 | 2012-06-13 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Aeromonas capable of removing hydrogen sulfide (H2S) gas in gas and usage thereof |
| CN105727707A (en) * | 2016-02-22 | 2016-07-06 | 北京沃太斯环保科技发展有限公司 | Device and method for treating odor through three-stage method |
-
2016
- 2016-12-23 CN CN201611204980.2A patent/CN106582258B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218421A (en) * | 1996-05-10 | 1999-06-02 | 帕克斯生物系统公司 | Purification of gases containing hydrogen sulphide |
| CN102438443A (en) * | 2009-03-20 | 2012-05-02 | 藻类科学公司 | System and method for treating wastewater via phototactic heterotrophic microorganism growth |
| CN102321548A (en) * | 2011-08-01 | 2012-01-18 | 浙江工业大学 | Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide |
| CN102424804A (en) * | 2011-12-14 | 2012-04-25 | 青岛思普润水处理有限公司 | Citrobacter sp. for removing H2S gas from gas, and use thereof |
| CN102492640A (en) * | 2011-12-14 | 2012-06-13 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Aeromonas capable of removing hydrogen sulfide (H2S) gas in gas and usage thereof |
| CN105727707A (en) * | 2016-02-22 | 2016-07-06 | 北京沃太斯环保科技发展有限公司 | Device and method for treating odor through three-stage method |
Non-Patent Citations (2)
| Title |
|---|
| 李志章等: "硫化氢气体的生物脱除方法研究", 《昆明冶金高等专科学校学报》 * |
| 王玲玲等: "《高效异养脱臭菌的筛选及鉴定》", 《辽宁化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384904A (en) * | 2017-09-11 | 2017-11-24 | 山东大学 | It is a kind of to be used to go immobilization heterotrophic microorganism of sulphide removal and preparation method and application |
| CN107384904B (en) * | 2017-09-11 | 2020-11-20 | 山东大学 | Immobilized heterotrophic microorganism for removing sulfide and preparation method and application thereof |
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