CN107164264A - Multiple-effect composite bacteria agent and application thereof - Google Patents
Multiple-effect composite bacteria agent and application thereof Download PDFInfo
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- CN107164264A CN107164264A CN201710412390.7A CN201710412390A CN107164264A CN 107164264 A CN107164264 A CN 107164264A CN 201710412390 A CN201710412390 A CN 201710412390A CN 107164264 A CN107164264 A CN 107164264A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
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- C02F2101/101—Sulfur compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The present invention relates to a kind of multiple-effect composite bacteria agent and application thereof.It is formed multiple-effect composite bacteria agent by polynary bacterium with synergist mixed fermentation, and the volume ratio of the polynary bacterium and the synergist is 1：1；Wherein, polynary bacterium bag includes lactic acid bacteria, saccharomycete, photosynthetic bacteria, bacillus, acetic acid bacteria, Bifidobacterium Bifidum；Synergist includes potassium dihydrogen phosphate, urea, molasses, calcium chloride, malic acid, compound amino acid, trace element, vitamin etc..Beneficial effects of the present invention are：It can be applied to the fields such as pond culture, flowers, plantation, rubbish deodorization, cultivation muck fermentation; nutrition decomposition and inversion unnecessary in environment is utilized; play decomposition and inversion for nutrient that is superfluous or can not directly utilizing; nutrition can also be seized with harmful bacteria, a large amount of inputs form dominant microflora.
Description
Technical field
The invention belongs to bacteria agent field, and in particular to a kind of multiple-effect composite bacteria agent and application thereof.
Background technology
With the fast development and the increasingly raising of living standards of the people of China's economy, urban wastewater discharge significantly increases
It is many.Substantial amounts of percolate, excreta, the discharge of slurry and the expansion of industrial production scale, directly contribute city
The pollutant load such as nitrogen phosphate and sulfur greatly improves in sewage.How to effectively reduce in environment, it is organic in such as soil, water body
The content of pollutant, is always the key problem curbed environmental pollution.Meanwhile, similar pollution problem exists in aquatic products
In terms of cultivation, livestock and poultry cultivation, fertilizer application, air.
And during handling now, it is high transport difficult, cost of transportation often occur, reagent easy in inactivation, use cost be high,
Consumption does not reach, DeGrain, transport are damaged, situations such as not easy to operate.
The content of the invention
In order to solve the problem of prior art is present, the invention provides a kind of multiple-effect composite bacteria agent and application thereof, it has
There is convenient transportation, be effectively improved environment, using the fermentation activation of ground scene, consumption be bigger, bacterium active stronger, action effect is brighter
The features such as aobvious, easy to operate etc..
It is an object of the invention to provide a kind of multiple-effect composite bacteria agent.
According to the multiple-effect composite bacteria agent of the specific embodiment of the invention, it is made up of polynary bacterium and synergist, wherein,
The polynary bacterium bag is included：The weight % of lactic acid bacteria 48~55, the weight % of saccharomycete 28~35, the weight of photosynthetic bacteria 5~10
Measure %, the weight % of bacillus 1~5, the weight % of acetic acid bacteria 1~3, the weight % of Bifidobacterium Bifidum 8~12；
The synergist includes：The weight % of potassium dihydrogen phosphate 15~25, the weight % of urea 4~6, the weight of molasses 55~70
Measure %, the weight % of calcium chloride 1~2, the weight % of malic acid 1~3, the weight % of compound amino acid 7~10, trace element 0.3~0.6
Weight %, the weight % of vitamin 0.3~0.6.
According to the multiple-effect composite bacteria agent of the specific embodiment of the invention, the trace element further comprises that concentration is sulfuric acid
1~3g/L of ferrous iron, 0.5~1.5g/L of boric acid, 0.3~0.6g/L of cobalt chloride, 0.1~0.3g/L of zinc chloride, manganese chloride 0.1~
0.2g/L, 0.1~0.2g/L of sodium molybdate, 0.1~0.2g/L of nickel chloride, copper chloride 0.1~0.2g/L and EDTA-2Na 3~
5g/L。
According to the multiple-effect composite bacteria agent of the specific embodiment of the invention, the vitamin further comprises that concentration is vitamin
0.5~1.5g/L of B6,1~3g/L of vitamin B1,4~6g/L of vitamin B3,1~3g/L of p-aminobenzoic acid, biotin
0.5~1.5g/L, 1~3g/L of 0.5~1.5g/L of vitamin B12 and calcium pantothenate.
According to the multiple-effect composite bacteria agent of the specific embodiment of the invention, the compound amino acid further comprises that concentration is bad
90~110g/L of propylhomoserin, 40~60g/L of glutamic acid, 15~25g/L of aspartic acid, 25~40g/L of alanine, glycine 5~
15g/L, 2~10g/L of 8~15g/L of tryptophan and threonine.
According to the multiple-effect composite bacteria agent of the specific embodiment of the invention, also contain the weight of peptone 1~3 in the synergist
Measure %.
It is compound according to the multiple-effect of the specific embodiment of the invention according to the multiple-effect composite bacteria agent of the specific embodiment of the invention
Also contain the weight % of yeast extract 1~2 in microbial inoculum, the synergist.
Present invention also offers purposes of the multiple-effect composite bacteria agent in pond culture, rubbish deodorization, muck fermentation.
According to the purposes of the multiple-effect composite bacteria agent of the specific embodiment of the invention, take isometric polynary bacterium with it is described
Synergist, is fermented after adding water, and amount of water is 30~60 times of the amount of polynary bacterium.
According to the purposes of the multiple-effect composite bacteria agent of the specific embodiment of the invention, more than 10 DEG C can ferment, fermentation time
For 5~7 days.
The organic fertilizer of the present invention, which refers to, is mainly derived from plant and (or) animal, imposes on soil and is led using providing plant nutrient as it
The carbonaceous material of function is wanted, meets National Performance Criteria NY525-2012, chicken manure, cow dung or pig manure etc. can be specifically used.
Beneficial effects of the present invention are：
1. various microorganisms can form symbiosis flora in the multiple-effect composite bacteria agent of the present invention, the stability of a system is good, lactic acid
Between bacterium, saccharomycete, photosynthetic bacteria, bacillus, acetic acid bacteria, Bifidobacterium Bifidum can symbiosis for a long time, possess good balance, respectively
Bacterial strain is indispensable, and can not arbitrarily add other bacterial strains；
2. the synergist in the multiple-effect composite bacteria agent of the present invention provides a large amount of sufficient nutrition for polynary bacterium, make in polynary bacterium
Microorganism can amount reproduction within a short period of time, reach application concentration；
3. the present invention multiple-effect composite bacteria agent compound at the scene fermentation after be used for pond culture, rubbish deodorization, muck fermentation,
When in blade fertilising, sewage disposal, polynary bacterium can utilize the nutriment in environment, quickly be bred, and form dominant bacteria
Group, so as to effectively suppress the harmful microorganism breeding in environment, produces benefit materials；Ammonia in water environment, hydrogen sulfide can be controlled
Deng harmful substance contents, regulate and control culturing pool microbial ecological structure；Rise and decompose for nutrient that is superfluous or can not directly utilizing
Transformation, can also seize nutrition with harmful bacteria, form dominant microflora；
4. in garbage disposal application, by the multiple-effect composite bacteria agent spray of the present invention on the surface of rubbish, polynary bacterium utilizes
Nutritional ingredient in rubbish is bred, with the stench phenomenon seized with harmful smelly bacterium that vomits in nutrition, reduction rubbish；Meanwhile,
The temperature produced in bacterial classification and fermentation process kills the worm's ovum in rubbish, effectively suppresses the taste of harmful mosquito from root
It is raw；Can also effective fermented organic, the nutrition in rubbish is preferably fermented into biological organic fertilizer and inhaled for floor vegetation
Receive and utilize；
5. the multiple-effect composite bacteria agent of the present invention is sprayed in plant leaf surface, the generation of plant chlorophyll can be stimulated, accelerates light
Cooperation is used；
6. the multiple elements design microbial inoculum application method of the present invention is simple and convenient, user is mixed polynary bacterium, synergist using preceding
Close and add after a certain amount of water by fermentation, that is, obtain a large amount of microbial bacterial agents, concentration when both effective guarantee is applied, and it is effectively save
The cost of long-distance transport.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belongs to the scope that the present invention is protected.
Embodiment 1
The multiple-effect composite bacteria agent of the present invention includes polynary bacterium and synergist, the polynary bacterium and the volume ratio of the synergist
For 1：1；Wherein,
Polynary bacterium：Lactic acid bacteria 40g, saccharomycete 20g, photosynthetic bacteria 5g, bacillus 1g, acetic acid bacteria 1g, Bifidobacterium Bifidum 8g,
It is dissolved in 1L water；
Synergist：It is potassium dihydrogen phosphate 15g, urea 4g, molasses 50g, calcium chloride 1g, malic acid 1g, compound amino acid 7g, micro-
Secondary element 0.3g, vitamin 0.3g, are dissolved in 1L water；
Wherein, compound amino acid contain lysine 90g/L, glutamic acid 40g/L, aspartic acid 15g/L, alanine 25g/L,
Glycine 5g/L, tryptophan 8g/L, threonine 2g/L；
Trace element contains ferrous sulfate 1g/L, boric acid 8g/L, cobalt chloride 0.3g/L, zinc chloride 0.1g/L, manganese chloride
0.05g/L, sodium molybdate 0.05g/L, nickel chloride 0.05g/L, copper chloride 0.05g/L, EDTA-2Na 3g/L；
Vitamin contains vitamin B6 0.5g/L, vitamin B1 1g/L, vitamin B3 4g/L, p-aminobenzoic acid 1g/
L, biotin 0.5g/L, vitamin B12 0.5g/L, calcium pantothenate 1g/L.
The application method of this multiple-effect composite bacteria agent is：
Polynary bacterium 550ml, synergist 550ml are taken, is placed in fermentation tank, add water 20kg30-60kg, is well mixed, is carried out
Sealed fermenting, more than 10 DEG C of environment temperature can be fermented, and once, fermentation is produced after 5 days for stirring in 2 days.
It is noted that the polynary bacterium of the present invention is with synergist fermentation process, being not required to additionally control temperature, the temperature of reaction
Spend the heat discharged in itself entirely from fermentation.Therefore, to fermentation without harsh requirement, process is easy, easily operated.
Embodiment 2
The multiple-effect composite bacteria agent of the present invention includes polynary bacterium and synergist, the polynary bacterium and the volume ratio of the synergist
For 1：1；Wherein,
Polynary bacterium：Lactic acid bacteria 50g, saccharomycete 30g, photosynthetic bacteria 6g, bacillus 2g, acetic acid bacteria 2g, Bifidobacterium Bifidum
10g, is dissolved in 1L water；
Synergist：Potassium dihydrogen phosphate 20g, urea 5g, molasses 60g, calcium chloride 1.5g, malic acid 2g, compound amino acid 8g,
Micro- 0.5g, vitamin 0.5g, are dissolved in 1L water；
Wherein, compound amino acid contains lysine 100g/L, glutamic acid 50g/L, aspartic acid 20g/L, alanine 30g/
L, glycine 10g/L, tryptophan 10g/L, threonine 5g/L；
Trace element contains ferrous sulfate 2g/L, boric acid 1g/L, cobalt chloride 0.5g/L, zinc chloride 0.2g/L, manganese chloride
0.1g/L, sodium molybdate 0.1g/L, nickel chloride 0.1g/L, copper chloride 0.1g/L, EDTA-2Na 4g/L；
Vitamin contain vitamin B6 1g/L, vitamin B1 2g/L, vitamin B3 5g/L, p-aminobenzoic acid 2g/L,
Biotin 1g/L, vitamin B12 1g/L, calcium pantothenate 2g/L.
The application method of this multiple-effect composite bacteria agent is：
Polynary bacterium 550ml, synergist 550ml are taken, is placed in fermentation tank, the 30-60kg that adds water is well mixed, closed hair is carried out
Ferment, more than 10 DEG C of environment temperature can be fermented, and once, fermentation is produced after 6 days for stirring in every 2 days.
Embodiment 3
The multiple-effect composite bacteria agent of the present invention includes polynary bacterium and synergist, the polynary bacterium and the volume ratio of the synergist
For 1：1；Wherein,
Polynary bacterium：Lactic acid bacteria 60g, saccharomycete 40g, photosynthetic bacteria 7g, bacillus 3g, acetic acid bacteria 3g, Bifidobacterium Bifidum
12g, is dissolved in 1L water；
Synergist：Potassium dihydrogen phosphate 25g, urea 6g, molasses 70g, calcium chloride 2g, malic acid 3g, compound amino acid 10g,
Micro- 0.6g, vitamin 0.6g, are dissolved in 1L water；
Wherein, compound amino acid contains lysine 110g/L, glutamic acid 60g/L, aspartic acid 25g/L, alanine 40g/
L, glycine 15g/L, tryptophan 15g/L, threonine 10g/L；
Trace element contains ferrous sulfate 3g/L, boric acid 1.2g/L, cobalt chloride 0.8g/L, zinc chloride 0.3g/L, manganese chloride
0.15g/L, sodium molybdate 0.15g/L, nickel chloride 0.15g/L, copper chloride 0.15g/L, EDTA-2Na 4g/L；
Vitamin contains vitamin B6 1.5g/L, vitamin B1 1g/L, vitamin B3 6g/L, p-aminobenzoic acid 3g/
L, biotin 1.5g/L, vitamin B12 1.5g/L, calcium pantothenate 3g/L.
The application method of this multiple-effect composite bacteria agent is：
Polynary bacterium 550ml, synergist 550ml are taken, is placed in fermentation tank, add water 30-60kg, is well mixed, is carried out closed
Fermentation, more than 10 DEG C of environment temperature can be fermented, and stirring in every 2 days once, is produced after fermenting 7 days.
The dosage of multiple elements design microbial inoculum of the present invention is：
During applied in pond culture：Multiple-effect composite bacteria agent after a set of fermentation of the every 10 mu of administrations of common cultivating pool；
Multiple-effect composite bacteria agent if for extraordinary pond (such as cultivation shrimp, crab) after a set of fermentation of every 5 mu of administrations；
During applied to sewage disposal：The consumption of the multiple-effect composite bacteria agent of the present invention for sewage total amount one thousandth to percentage
One of；
During applied to rubbish deodorization：The consumption of the multiple-effect composite bacteria agent of the present invention is the one thousandth of rubbish total amount, sprinkling
In rubbish surface；
When being fermented applied to muck：The consumption of the multiple-effect composite bacteria agent of the present invention is the one thousandth of muck total amount to thousand points
Two；It is to be noted that water tariff collection fermentation results at 60% are ideal in fermentation process.Can fast decoupled in fermentation
Organic matter, crude fibre in organic fertilizer, improve the fertilizer efficiency such as nitrogen, phosphorus, potassium, amine, reduce pollution.
During applied to foliage application：Used after stoste after the multiple-effect composite bacteria fermentation of the present invention is diluted into 10,000 times.
The consumption of each component of 1 embodiment of table 4~8
The product of contaminated river, respectively Example 1,2,3,4,6,8 is gathered, according to multiple-effect composite bacteria agent of the present invention
Application process handled, determine apply front and rear COD in water body, BOD respectively5, ammonia nitrogen numerical value change, specific data are shown in Table
2,
The variation of water statistical form of table 2
Conclusion：Using COD, BOD of front and rear water body5, ammonia nitrogen data be decreased obviously, illustrate the present invention multiple-effect compound bacteria
Agent can effectively decompose the nutriment in water body, purify water source.
Wherein, ammonia-nitrogen content is determined using nessler reagent spectrophotometer (HJ535-2009) in table 2, and total phosphorus uses water quality
Five-day BOD determination method (GB7488-87) is determined, and COD detections use potassium bichromate-Sulfuric-Acid Digestion method (GB11914-
89)。
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (9)
1. a kind of multiple-effect composite bacteria agent, it is characterised in that：The multiple-effect composite bacteria agent is made up of polynary bacterium and synergist, wherein,
The polynary bacterium bag is included：The weight % of lactic acid bacteria 48~55, the weight % of saccharomycete 28~35, the weight % of photosynthetic bacteria 5~10,
The weight % of bacillus 1~5, the weight % of acetic acid bacteria 1~3, the weight % of Bifidobacterium Bifidum 8~12；
The synergist includes：The weight % of potassium dihydrogen phosphate 15~25, the weight % of urea 4~6, the weight % of molasses 55~70, chlorine
Change calcium 1~2 weight %, the weight % of malic acid 1~3, the weight % of compound amino acid 7~10,0.3~0.6 weight % of trace element,
The weight % of vitamin 0.3~0.6.
2. multiple-effect composite bacteria agent according to claim 1, it is characterised in that：It is described trace element include ferrous sulfate 1~
3g/L, 0.5~1.5g/L of boric acid, 0.3~0.6g/L of cobalt chloride, 0.1~0.3g/L of zinc chloride, 0.1~0.2g/L of manganese chloride, molybdenum
Sour 0.1~0.2g/L of sodium, 0.1~0.2g/L of nickel chloride, 3~5g/L of copper chloride 0.1~0.2g/L and EDTA-2Na.
3. multiple-effect composite bacteria agent according to claim 1, it is characterised in that：The vitamin include vitamin B6 0.5~
1.5g/L, 1~3g/L of vitamin B1,4~6g/L of vitamin B3,1~3g/L of p-aminobenzoic acid, 0.5~1.5g/ of biotin
L, 1~3g/L of 0.5~1.5g/L of vitamin B12 and calcium pantothenate.
4. multiple-effect composite bacteria agent according to claim 1, it is characterised in that：The compound amino acid include lysine 90~
110g/L, 40~60g/L of glutamic acid, 15~25g/L of aspartic acid, 25~40g/L of alanine, 5~15g/L of glycine, color ammonia
2~10g/L of acid 8~15g/L and threonine.
5. multiple-effect composite bacteria agent according to claim 1, it is characterised in that：Also contain peptone 1~3 in the synergist
Weight %.
6. multiple-effect composite bacteria agent according to claim 1, it is characterised in that：Also contain yeast extract 1~2 in the synergist
Weight %.
7. the multiple-effect composite bacteria agent according to any one of claim 1~6 is in pond culture, rubbish deodorization, muck fermentation, leaf
Purposes in piece fertilising, sewage disposal.
8. purposes according to claim 7, it is characterised in that：Isometric polynary bacterium and the synergist are taken, plus
Fermented after water, amount of water is 30~60 times of the amount of polynary bacterium.
9. the purposes of multiple-effect composite bacteria agent according to claim 8, it is characterised in that：Fermentation time is 5~7 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586739A (en) * | 2017-09-30 | 2018-01-16 | 南京万德斯环保科技股份有限公司 | A kind of complex microbial inoculum for purifying river course water environment and its application |
| CN111592402A (en) * | 2020-05-11 | 2020-08-28 | 福建省农业科学院畜牧兽医研究所 | Method for improving fermentation efficiency of pig manure |
| CN112375721A (en) * | 2021-01-02 | 2021-02-19 | 吉林化工学院 | Preparation method and application of composite microbial inoculum for low-temperature treatment of rural domestic sewage |
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| CN112375721A (en) * | 2021-01-02 | 2021-02-19 | 吉林化工学院 | Preparation method and application of composite microbial inoculum for low-temperature treatment of rural domestic sewage |
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