CN107267409A - A kind of preparation method of environmental protection compound bacteria flora - Google Patents
A kind of preparation method of environmental protection compound bacteria flora Download PDFInfo
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- CN107267409A CN107267409A CN201710285207.1A CN201710285207A CN107267409A CN 107267409 A CN107267409 A CN 107267409A CN 201710285207 A CN201710285207 A CN 201710285207A CN 107267409 A CN107267409 A CN 107267409A
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- flora
- compound bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention provides a kind of preparation method of environmental protection compound bacteria flora, the main component of the compound bacteria flora is photosynthetic bacteria group, yeast flora, actinomycete group, acetobacter group, desulfurization flora, bacillus group and Bifidobacteria population, the special compound bacteria flora, effect of not only becoming thoroughly decomposed is good, and compare conventional method, time substantially shortens, and cost is low, and effect is good；The compound bacteria flora prepares the composite flora available for peculiar smell such as rubbish, the sewage produced in elimination environment；Soil texture, rehabilitating soil, beneficial to the development composite flora of planting industry can be improved；Purify water, promote organic pollution to decompose, the composite flora of BOD, COD content in reduction water.
Description
Technical field
The present invention relates to microorganism species preparing technical field, it particularly relates to a kind of environmental protection compound bacteria flora
Preparation method.
Background technology
Microbial manure is live body fertilizer, a large amount of beneficial microorganism vital movement generations that its effect contains mainly by it
Thank to complete.Only in the case that these beneficial microbes are in vigorous breeding and metabolism, material is converted and beneficial
Metabolite could be formed constantly.Therefore, it is that it has that whether beneficial microorganism species, vital movement are vigorous in microbial manure
The basis of effect property, rather than other fertilizer be by the form of the essential elements such as nitrogen, phosphorus, potassium and it is how many based on.Just because of micro- life
Thing fertilizer is preparation living, so its fertilizer efficiency and number of viable, intensity and ambient environmental conditions are closely related, including temperature, moisture,
Acid-base value, nutritional condition and original, which live in indigenous microorganism repulsive interaction in soil, certain influence, therefore will in application
It is noted that.
Microbial manure also have some other fertilizer without special role.Now it is described as follows：
A large amount of with chemical fertilizer use, and its utilization rate constantly reduces the fact that be generally well known.This explanation, only by largely increasing
Chemical fertilizer still more also has a series of problem of pollution environment etc. to improve crop yield be limited.For this scientists from all over the world one
It is straight to reach balance fertilizing, the rational application of fertilizer to overcome the approach of its drawback in effort exploration raising chemical fertilizer utilization ratio.Microbial manure
Play the role of on this respect is solved the problems, such as original.So, according to Crops In China species and edaphic condition, using microbial manure
With chemical fertilizer compounding application, volume increase had both been can guarantee that, fertilizer application amount is reduced again, cost is reduced, while soil and work can also be improved
Article matter, reduces pollution.
With the requirement that the continuous improvement of living standards of the people, especially people improve to quality of life, the whole world is all in product
Pole development green agriculture (sustainable agriculture) comes production safety, non-harmful pollution-free food.Will during production pollution-free food
Ask without or try one's best and be few with (or limitation is used) chemical fertilizer, chemical pesticide and other chemical substances.It requires that fertilizer must be first
First protect and promote subject growth and improve quality；Secondly subject is not caused to produce and accumulation harmful substance；Three are
Ecological environment is had no adverse effects.Microbial manure substantially conforms to the principle of the above three.In recent years, China is with specific function
Strain multiple-microorganism fertilizer is made, can not only relax or reduce pollution of agricultural products, and the quality of agricultural product can be improved.
Microorganism fertilizer is made using the specific function decomposing and fermenting domestic waste and agriculture wastes of microorganism
Material is an economically viable effective way.The mainly two methods applied at present, one is by substantial amounts of urban life rubbish
Rubbish is directly processed into microbial organic complex fertilizer through handling as raw material by factory；Two be the special microbial manure of plant produced
(inoculum agent) is supplied in composting plant (field), then carries out composting to various farming and animal husbandry materials, to accelerate its fermentation process, shortens compost
Cycle, while also improving compost quality and maturity.Also have in addition and used microbial manure as soil sanitation agent.
Beneficial microbe can produce glucide in microbial manure, account for the 0.1% of the soil organism, and plant mucilage,
Mineral idiosome and organic colloid are combined together, and can improve soil granular structure, are strengthened the physical property of soil and are reduced soil
The loss of earth particle, under certain conditions, moreover it is possible to participate in humus and formed.So soil thing can be improved using microbial manure
Rationality shape, is conducive to increasing soil fertility.
In recent years, stalk comprehensive utilization rate is 70.6%, about 500,000,000 tons of utilization.Wherein, as feed usage amount about 2.18 hundred million
Ton, accounts for 31.9%；As about 1.07 hundred million tons of Fertilizer application amount (being free of stubble in field, about 1.58 hundred million tons of stubble in field amount), account for
15.6%；As about 0.18 hundred million tons of edible mushroom base-material amount of plantation, 2.6% is accounted for；As the raw material of industry such as wood-based plate, papermaking amount about
0.18 hundred million tons, account for 2.6%；It is used as fuel usage amount (containing the traditional cooking heating of peasant household, the utilization of stalk novel energy) about 1.22
Hundred million tons, account for 17.8%.Stalk is extended to fertilizer, feed, edible mushroom base by being used only as rural waste and animal feeding-stuff in the past
The purposes such as material, the raw material of industry and fuel；Modern industry, energy field are developed into by past traditional agriculture field.Stalk energy
Using there occurs certain change, develop into stalk direct combustion generating, stalk marsh gas, straw solidification, stalk from peasant's inefficient combustion and do
Evaporate etc. and efficiently to utilize.By carrying forward vigorously stalk comprehensive utilization, related industry is driven to accelerate development, the crop straw burning of key area
Problem is substantially achieved solution, and air environmental pollution problem is effectively alleviated, and has driven surplus rural labor force's employment, has promoted
Growth of agricultural efficiency and increasing peasant income.The stalk of the consumption of stock keeping in 2010 is equivalent to saving food 50,000,000 tons；Phase is used as fuel
When in saving about 60,000,000 tons of coal of mark, the multi-win of environmental benefit, economic benefit and social benefit is realized.
But in traditional technique, the comprehensive utilization ratio of stalk still has much room for improvement, the speed of becoming thoroughly decomposed of especially stalk is special
Slowly, as fertilizer, on the one hand straw decomposing speed is slow to cause crop growth Stage Nutrition insufficient, and another invention can also
Cause various pest infestations, crops are caused with long-term hazards, therefore be necessary to study a kind of technique of efficiently becoming thoroughly decomposed of stalk.
The content of the invention
In view of this, it is an object of the invention to provide a kind of environmental protection preparation method of compound bacteria flora, this method is significantly
Improve the efficiency of becoming thoroughly decomposed of stalk.
A kind of environmental protection preparation method of compound bacteria flora, comprises the following steps：
A, by photosynthetic bacteria, saccharomycete, actinomyces, acetobacter, desulfurization bacterium, bacillus and Bifidobacterium, through inclined-plane and shaking flask
Cultivate, enter back into after seed tank culture and to produce liquid compound bacteria flora in industry situation fermentation cylinder for fermentation；
B, by freeze-drying powdered compound bacteria flora is made in liquid bacteria sample；
C, powdered compound bacteria flora is ground, obtains micron powder shape bacterium sample；
D, micron powder shape bacterium sample packed, you can.
It is preferred that, described compound bacteria flora, the content of each flora is 109cfu/g。
It is preferred that, in described compound bacteria flora, the percentage by weight of each flora is：Photosynthetic bacteria group 16-25%, saccharomycete
Group 10-25%, actinomycete group 15-30%, acetobacter group 3-12%, desulfurization flora 15-26%, bacillus group 3-8% and
Bifidobacteria population 2-7%.
It is preferred that, the compound bacteria flora can be used for preparing straw decomposing material, and it is resistant to lodging that straw decomposing material can be applied to wheat
Microbial manure.
It is preferred that, compound bacteria flora is used for during preparing straw decomposing material, the time control of compost maturity is in 4-6
It, nearly 1/3 time is shortened than conventional method.
It is preferred that, described wheat microbial manure resistant to lodging, including following raw material：Environmental protection compound bacteria flora, nitrogen phosphorus
Potash fertilizer and water.
It is preferred that, described wheat microbial manure resistant to lodging includes the raw material of following percentage by weight：Straw decomposing material
70-80%, azophoska 8-15% and water surplus.
The preparation method of environmental protection compound bacteria flora provided by the present invention, the main component of the compound bacteria flora is photosynthetic
Flora, yeast flora, actinomycete group, acetobacter group, desulfurization flora, bacillus group and Bifidobacteria population, this is special to answer
Bacterium flora is closed, not only the effect of becoming thoroughly decomposed to stalk is good, and than conventional method, the time substantially shortens, and cost is low, and effect is good；Should
Compound bacteria flora prepares the composite flora available for peculiar smell such as rubbish, the sewage produced in elimination environment；Soil texture can be improved,
Rehabilitating soil, beneficial to the development composite flora of planting industry；Purify water, promote organic pollution to decompose, BOD, COD in reduction water
The composite flora of content.
Embodiment
Following examples, to further labor of the invention：
Embodiment 1
A kind of environmental protection preparation method of compound bacteria flora, comprises the following steps：
A, by photosynthetic bacteria, saccharomycete, actinomyces, acetobacter, desulfurization bacterium, bacillus and Bifidobacterium, through inclined-plane and shaking flask
Cultivate, enter back into after seed tank culture and to produce liquid compound bacteria flora in industry situation fermentation cylinder for fermentation；
B, by freeze-drying powdered compound bacteria flora is made in liquid bacteria sample；
C, powdered compound bacteria flora is ground, obtains micron powder shape bacterium sample；
D, micron powder shape bacterium sample packed, you can.
Described compound bacteria flora, the content of each flora is 109cfu/g。
In described compound bacteria flora, the percentage by weight of each flora is：Photosynthetic bacteria group 20%, yeast flora 18%, put
Line flora 19%, acetobacter group 10%, desulfurization flora 22%, bacillus group 6% and Bifidobacteria population 5%.
Embodiment 2
A kind of environmental protection preparation method of compound bacteria flora, comprises the following steps：
A, by photosynthetic bacteria, saccharomycete, actinomyces, acetobacter, desulfurization bacterium, bacillus and Bifidobacterium, through inclined-plane and shaking flask
Cultivate, enter back into after seed tank culture and to produce liquid compound bacteria flora in industry situation fermentation cylinder for fermentation；
B, by freeze-drying powdered compound bacteria flora is made in liquid bacteria sample；
C, powdered compound bacteria flora is ground, obtains micron powder shape bacterium sample；
D, micron powder shape bacterium sample packed, you can.
Described compound bacteria flora, the content of each flora is 109cfu/g。
In described compound bacteria flora, the percentage by weight of each flora is：Photosynthetic bacteria group 16%, yeast flora 25%, put
Line flora 25%, acetobacter group 12%, desulfurization flora 15%, bacillus group 5% and Bifidobacteria population 2%.
Embodiment 3
A kind of environmental protection preparation method of compound bacteria flora, comprises the following steps：
A, by photosynthetic bacteria, saccharomycete, actinomyces, acetobacter, desulfurization bacterium, bacillus and Bifidobacterium, through inclined-plane and shaking flask
Cultivate, enter back into after seed tank culture and to produce liquid compound bacteria flora in industry situation fermentation cylinder for fermentation；
B, by freeze-drying powdered compound bacteria flora is made in liquid bacteria sample；
C, powdered compound bacteria flora is ground, obtains micron powder shape bacterium sample；
D, micron powder shape bacterium sample packed, you can.
Described compound bacteria flora, the content of each flora is 109cfu/g。
In described compound bacteria flora, the percentage by weight of each flora is：Photosynthetic bacteria group 25%, yeast flora 10%, put
Line flora 26%, acetobacter group 3%, desulfurization flora 26%, bacillus group 3% and Bifidobacteria population 7%.
Comparative example 1
Desulfurization bacterium in the strain of embodiment 1 is removed, remaining preparation condition is constant.
Compost maturity time following to the material that become thoroughly decomposed in embodiment 1-3 and the microbial manure of comparative example 1 and applied to small
Wheat microbial manure resistant to lodging, the lodging rate of wheat is detected, obtains following data：
Described wheat microbial manure resistant to lodging, includes the raw material of following percentage by weight：Become thoroughly decomposed material 75%, azophoska
10% and water surplus.
| The compost maturity time (hour) | The lodging rate % of wheat | |
| Embodiment 1 | 105 | 2.5 |
| Comparative example 1 | 186 | 12.8 |
By above test data it is recognised that due to not adding desulfurization bacterium, the compost maturity time of microbial manure is notable
Increase, adds after desulfurization bacterium, can significantly improve the lodging tolerance of wheat.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (6)
1. a kind of preparation method of environmental protection compound bacteria flora, it is characterised in that comprise the following steps：
A, by photosynthetic bacteria, saccharomycete, actinomyces, acetobacter, desulfurization bacterium, bacillus and Bifidobacterium, through inclined-plane and shaking flask
Cultivate, enter back into after seed tank culture and to produce liquid compound bacteria flora in industry situation fermentation cylinder for fermentation；
B, by freeze-drying powdered compound bacteria flora is made in liquid bacteria sample；
C, powdered compound bacteria flora is ground, obtains micron powder shape bacterium sample；
D, micron powder shape bacterium sample packed, you can.
2. the preparation method of environmental protection compound bacteria flora as claimed in claim 1, it is characterised in that described compound bacteria bacterium
Group, the content of each flora is 109cfu/g。
3. the preparation method of environmental protection compound bacteria flora as claimed in claim 1, it is characterised in that described compound bacteria flora
In, the percentage by weight of each flora is：Photosynthetic bacteria group 16-25%, yeast flora 10-25%, actinomycete group 15-30%, acetic acid bar
Flora 3-12%, desulfurization flora 15-26%, bacillus group 3-8% and Bifidobacteria population 2-7%.
4. the preparation method of environmental protection compound bacteria flora as claimed in claim 1, it is characterised in that the compound bacteria flora can use
In preparing straw decomposing material, straw decomposing material can be applied to wheat microbial manure resistant to lodging.
5. the preparation method of environmental protection compound bacteria flora as claimed in claim 4, it is characterised in that described wheat is resistant to lodging
Microbial manure, including following raw material：Straw decomposing material, azophoska and water.
6. the preparation method of environmental protection compound bacteria flora as claimed in claim 5, it is characterised in that described wheat is resistant to lodging
Microbial manure, includes the raw material of following percentage by weight：More than straw decomposing material 70-80%, azophoska 8-15% and water
Amount.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107879774A (en) * | 2017-11-08 | 2018-04-06 | 常州文诺纺织品有限公司 | A kind of preparation method of biological decomposing agent |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN115611671A (en) * | 2022-09-26 | 2023-01-17 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219570A (en) * | 2011-05-09 | 2011-10-19 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
-
2017
- 2017-04-27 CN CN201710285207.1A patent/CN107267409A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219570A (en) * | 2011-05-09 | 2011-10-19 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
Non-Patent Citations (1)
| Title |
|---|
| 张洪生: "KJ菌剂发酵秸秆饲料的制作方法及应用", 《中国农村小康科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107879774A (en) * | 2017-11-08 | 2018-04-06 | 常州文诺纺织品有限公司 | A kind of preparation method of biological decomposing agent |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN115611671A (en) * | 2022-09-26 | 2023-01-17 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
| CN115611671B (en) * | 2022-09-26 | 2023-10-27 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
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Application publication date: 20171020 |