CN1404735A - Compound microbiological bacterial agent and its production method - Google Patents
Compound microbiological bacterial agent and its production method Download PDFInfo
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- CN1404735A CN1404735A CN 01127096 CN01127096A CN1404735A CN 1404735 A CN1404735 A CN 1404735A CN 01127096 CN01127096 CN 01127096 CN 01127096 A CN01127096 A CN 01127096A CN 1404735 A CN1404735 A CN 1404735A
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- micro organism
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- organism fungicide
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Abstract
The present invention relates to a compound microbial bacterial preparation for treating sewage and its production method. It is mainly formed from photosynthetic bacterium, lactobacillus, actinomycetes and zymogenous filamentous bacteria. The said compound microbial bacteria are inoculated into the culture medium to make enrichment culture so as to obtain the invented compound microbial bacterial preparation for completely treating sewage, and its cost is low.
Description
The present invention relates to a kind of complex micro organism fungicide and the production method thereof of sewage treatment area.
Relevant by retrieval patent documentation does not retrieve complex micro organism fungicide and the production method thereof to treatment of Organic Wastewater identical with the present invention at present as yet.Existing processing to high concentrated organic wastewater generally adopt anaerobism, aerobic combined method, film separated activated sludge, granulating activated sludge process to wait and handle, but these technology ranges of application is narrower, and regulation effect is not ideal.China's patent application, application number is " 98111936.0 ", name is called " process for treating microorganism in mud and sewage that adopts wooden flakes ", disclose a kind of in treatment trough with blow vent, Treatment of Sludge water inlet and Treatment of Sludge water outlet, pack grain wooden flakes treatment media layer reaches the method for handling organic waste water, though this application plays certain positive role to the processing of sewage, but because the granule medium that is thrown into has secondary pollution equally, and need often augment medium, so bring a lot of inconvenience for the processing of sewage.
The objective of the invention is to provide that a kind of employing is aerobic, anaerobism, the oxygen quasi-microorganism of holding concurrently form through Mixed culture, the organic matter in the degrading waste water makes it reach a kind of complex micro organism fungicide of discharge standard effectively.
Another object of the present invention is that a kind of method of producing above-mentioned complex micro organism fungicide will be provided.
Technical characterictic of the present invention is: consider can be effectively to treatment of Organic Wastewater, reduce on the basis of waste water treatment expense, be designed to mainly by photosynthetic bacteria, lactic acid bacteria, actinomycetes, the mold fungi of fermentation system, the complex micro organism fungicide that acetobacter is formed, the content of described each bacterial classification according to the composition of processed organic waste water regulate, proportioning.
It adopts and grows respectively in medium into the mentioned microorganism bacterial strain, injects the mixed culture medium enrichment culture and form after cultivating propagation respectively.
Described mixed culture medium adopts the cellulose of the 65-85% of adding of required processing waste water own, the lignin of 10-25%, the carbonaceous filtering agent that the penta poly-acid of 5-10% and rice bran, oyster shell, living lotus flower, perennial ryegrass, the mixture of eucalyptus skin after fragmentation constitute, make it reach the material of the somatomedin of the required 75-80% of above-mentioned complex micro organism fungicide through fermentation again, described cultivation temperature t is: 3-50 ℃.
Photosynthetic bacteria in the described complex micro organism fungicide belongs to the autotrophy microorganism, it is an energy to accept light and heat, with organic matter or pernicious gas is matrix, several physiological active substances such as nitrogen-containing compound such as synthesizing amino acid, nucleic acid and carbohydrate, these metabolites, the matrix that becomes other microbial reproduction that has; The carbohydrate that described lactic acid bacteria obtains with photosynthetic bacteria, saccharomycete is a matrix, produces lactic acid.Because lactic acid bacteria has very strong sterilizing power, particularly suppress the harmful microbe breeding, and organic rapid corrupt the decomposition; Lactic acid bacteria can make the difficult organic matter that decomposes such as lignin, cellulose decompose; Actinomycetes in the described complex micro organism fungicide, it can absorb the amino acid that photosynthetic bacteria produces, produce antibiotics, this antibiont mass-energy effectively suppresses pathogen, and the desired material when being harmful to mould and bacterial multiplication of ingesting, thereby suppress its propagation, creation helps other microorganism survival environment; Because actinomycetes and photosynthetic bacteria coexistence, the clean bacterium effect that they are brought into play is multiplied when more independent than actinomycetes; Mold fungi in the described complex micro organism fungicide, the Eurotium that mainly is fermentation is a main body, this flora can coexist with other microorganism, the generation that promotes other effective microbe propagation matrix is played an important role, and have an effect of decomposing odorant, can with all corruption things and bacillus eats, carbonization; Acetobacter in the complex micro organism fungicide, main fermentation and the decomposition of being responsible for animals and plants remains, remains and most organic substances.
Described complex micro organism fungicide is cultivated down and is bred the microbial inoculum that and can effectively handle organic waste water and solid life refuse under the low-temperature condition being lower than 10 ℃.
Described complex micro organism fungicide is bred the microbial inoculum that 40-50 ℃ of following cultivation and can handle organic waste water and solid life refuse under 60-70 ℃ of situation.
During use, complex micro organism fungicide of the present invention is rendered in the biochemistry pool, the organic matter of handling with need reaches the purpose of the treatment of wastes with processes of wastes against one another as medium, and non-secondary pollution.Water outlet after oxidation Decomposition, precipitation process reaches discharge standard.
Advantage of the present invention is: owing to adopt the mode of multiple bacterial classification combination, organic waste water is handled, avoided because of the no thoroughness of single culture to waste water treatment; Because described microbial inoculum is simple for production, cost is low, thereby has reduced the cost of waste water treatment; Because obvious to the treatment of Organic Wastewater effect, facility is simple, and is easy to operate, so very big practical and popularizing value is arranged.
The present invention is described further below with reference to embodiment:
1, Nantong pharmacy head factory is mainly produced chemical synthetic drug, and product has Primidone, phenylbutazone, fluorouracil, feldene and supporting intermediate Ethyl formate.In process of production Pai Fang high-concentration waste water CODcr mean value up to 200,000 mg/L about, adopt the present invention to handle in August, 1999, its method is: described each microbial strains is grown in the medium respectively bred.Get the cellulose of required processing waste water adding 85%, 10% lignin, the mixture of 5% penta poly-acid and rice bran, oyster shell, living lotus flower, perennial ryegrass, eucalyptus skin, after mixing, each microorganism fungus kind is grown in the medium under 25 ℃, cultivate after 3 days, make suspension with chlorine water, and enlarged culture step by step, then obtain described composite bacteria agent capable.When disposing of sewage, at first waste water is transferred pH value to 9, again sediment is carried out press filtration with plate case filter press to suspension in the sewage, the sewage after the press filtration is concentrated enter in the biochemistry pool, then described complex micro organism fungicide is dropped in the biochemistry pool, sewage is handled.The water outlet of raw wastewater after without dilution process detects COD through environmental administration
CrAverage removal rate is 80%.
Treatment of Organic Wastewater chemical oxygen demand (COD
Cr) the testing result statistical form
Unit: mg/L
| Date | Former water (weighted mean) | After the press filtration | After the processing |
| The total clearance (%) of annual average clearance on December 28 (%) in 1999 | ????1.49×10 5 | ??1.04×10 5??1.09×10 5??1.06×10 5??29 | ??4.09×10 4??4.18×10 4??4.14×10 4??61 ??72 |
| The total clearance (%) of annual average clearance on January 4 (%) in 2000 | ????1.96×10 5 | ??1.38×10 5??1.86×10 5??1.62×10 5??17 | ??2.59×10 4??3.16×10 4??4.14×10 4??82 ??85 |
| The total clearance (%) of two day average clearances (%) | ????1.72×10 5 | ??1.34×10 5??22 | ??3.51×10 4??74 ??80 |
2, dam, Rugao City bridge casing factory owner will be engaged in the processing of semi-finished product casing, check, produces heparin according to market conditions simultaneously, and this factory production waste water is mainly from operations such as rinsing, shunt and rehydrations.Adopt the present invention to handle, its method is: described each microbial strains is grown in the medium respectively bred.Get the cellulose of required processing waste water adding 75%, 15% lignin, the mixture of 10% penta poly-acid and rice bran, oyster shell, living lotus flower, perennial ryegrass, eucalyptus skin, after mixing, each microorganism fungus kind is grown in the medium under 25 ℃, cultivate after 3 days, make suspension with chlorine water, and enlarged culture step by step, then obtain described composite bacteria agent capable.Then described complex micro organism fungicide is dropped in the biochemistry pool, sewage is handled, the water outlet after the processing detects through environmental administration, and the result is:
Monitoring result (except unit: the mg/L, PH)
| The monitoring date | The sample title | ????PH | ????COD cr | ????SS | ????BOD 5 | ????NH 3-N | Treating capacity |
| 99.10.11 | Before the processing 1. | ????6.95 | ????795 | ????64 | ????292 | ????57.7 | ????12T/d |
| Before the processing 2. | ????6.98 | ????997 | ????67 | ????279 | ????52.9 | ||
| Before the processing 3. | ????7.02 | ????704 | ????62 | ????207 | ????42.0 | ||
| After the processing 1. | ????7.25 | ????84 | ????27 | ????24.1 | ????15.5 | ||
| After the processing 2. | ????7.28 | ????107 | ????26 | ????31.1 | ????20.4 | ||
| After the processing 3. | ????7.26 | ????112 | ????26 | ????31.8 | ????18.6 | ||
| 99.10.12 | Before the processing 4. | ????6.87 | ????833 | ????57 | ????240 | ????36.4 | ????12T/d |
| Before the processing 5. | ????6.92 | ????766 | ????60 | ????216 | ????27.6 | ||
| Before the processing 6. | ????6.71 | ????772 | ????62 | ????241 | ????31.7 | ||
| After the processing 4. | ????7.28 | ????102 | ????29 | ????32.0 | ????19.4 | ||
| After the processing 5. | ????7.29 | ????78 | ????28 | ????19.6 | ????19.7 | ||
| After the processing 6. | ????7.32 | ????88 | ????26 | ????25.5 | ????21.2 |
| ??99.10.13 | Before the processing 7. | ??7.02 | ??717 | ??62 | ??250 | ??30.3 | ??12T/d |
| Before the processing 8. | ??6.87 | ??631 | ??58 | ??217 | ??24.7 | ||
| Before the processing 9. | ??6.92 | ??447 | ??60 | ??151 | ??22.7 | ||
| After the processing 7. | ??7.19 | ??78 | ??26 | ??22.1 | ??22.0 | ||
| After the processing 8. | ??7.19 | ??106 | ??27 | ??36.0 | ??22.4 | ||
| After the processing 9. | ??7.21 | ??114 | ??28 | ??35.6 | ??26.4 | ||
| Average before handling | ??6.92 | ??740 | ??61 | ??233 | ??36.2 | ??12T/d | |
| Handle the back average | ??7.25 | ??97 | ??27 | ??28.6 | ??20.6 | ||
Claims (3)
1, a kind of complex micro organism fungicide is characterized in that: it mainly is made up of mold fungi, the acetobacter of photosynthetic bacteria, lactic acid bacteria, actinomycetes, fermentation system, the content of described each bacterial classification according to the composition of processed organic waste water regulate, proportioning.
2, a kind of production method of making the described complex micro organism fungicide of claim 1 is characterized in that: it adopts and grows respectively in medium into the mentioned microorganism bacterial strain, injects the mixed culture medium enrichment culture and form after cultivating propagation respectively.
3, the production method of complex micro organism fungicide according to claim 2, it is characterized in that: described mixed culture medium adopts the cellulose of the 65-85% of adding of required processing waste water own, the lignin of 10-25%, the carbonaceous filtering agent that the penta poly-acid of 5-10% and rice bran, oyster shell, living lotus flower, perennial ryegrass, the mixture of eucalyptus skin after fragmentation constitute, make it reach the material of the somatomedin of the required 75-80% of above-mentioned complex micro organism fungicide through fermentation again, described cultivation temperature t is: 3-50 ℃.
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| CN 01127096 CN1189555C (en) | 2001-08-13 | 2001-08-13 | Compound microbiological bacterial agent and its production method |
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| CN 01127096 CN1189555C (en) | 2001-08-13 | 2001-08-13 | Compound microbiological bacterial agent and its production method |
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| CN1404735A true CN1404735A (en) | 2003-03-26 |
| CN1189555C CN1189555C (en) | 2005-02-16 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN100415767C (en) * | 2006-05-22 | 2008-09-03 | 章浸冰 | Pollution-free production method for processing saponin from dioscorea zingibernensis |
| CN102586111A (en) * | 2012-02-28 | 2012-07-18 | 宗绪成 | Microorganism bacterium agent for subtracting organic sludge, production method of microorganism bacterium agent and complex culture medium |
| CN101701197B (en) * | 2009-09-30 | 2012-07-18 | 河南亚神环保科技有限公司 | Novel microorganism flora mixture and mixed nutrient medium thereof |
| CN103271095A (en) * | 2013-04-11 | 2013-09-04 | 山西省农业科学院果树研究所 | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
| CN105347481A (en) * | 2015-11-26 | 2016-02-24 | 山东大学 | Method for purifying low-carbon-nitrogen-ratio domestic sewage by biological fermentation coupling artificial wetland system |
| CN110272853A (en) * | 2019-07-25 | 2019-09-24 | 广东五色时空生态环境科技有限公司 | A kind of microbial culture method |
| CN110791447A (en) * | 2019-11-21 | 2020-02-14 | 河北建设集团安装工程有限公司 | Compound microbial agent for improving water body sediment and preparation method and application thereof |
-
2001
- 2001-08-13 CN CN 01127096 patent/CN1189555C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN100415767C (en) * | 2006-05-22 | 2008-09-03 | 章浸冰 | Pollution-free production method for processing saponin from dioscorea zingibernensis |
| CN101701197B (en) * | 2009-09-30 | 2012-07-18 | 河南亚神环保科技有限公司 | Novel microorganism flora mixture and mixed nutrient medium thereof |
| CN102586111A (en) * | 2012-02-28 | 2012-07-18 | 宗绪成 | Microorganism bacterium agent for subtracting organic sludge, production method of microorganism bacterium agent and complex culture medium |
| CN102586111B (en) * | 2012-02-28 | 2014-06-11 | 宗晓进 | Microorganism bacterium agent for subtracting organic sludge, production method of microorganism bacterium agent and complex culture medium |
| CN103271095A (en) * | 2013-04-11 | 2013-09-04 | 山西省农业科学院果树研究所 | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof |
| CN103271095B (en) * | 2013-04-11 | 2015-01-21 | 山西省农业科学院果树研究所 | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
| CN105347481A (en) * | 2015-11-26 | 2016-02-24 | 山东大学 | Method for purifying low-carbon-nitrogen-ratio domestic sewage by biological fermentation coupling artificial wetland system |
| CN105347481B (en) * | 2015-11-26 | 2017-11-03 | 山东大学 | The method that biofermentation coupling artificial wet land system purifies low ratio of carbon to ammonium sanitary sewage |
| CN110272853A (en) * | 2019-07-25 | 2019-09-24 | 广东五色时空生态环境科技有限公司 | A kind of microbial culture method |
| CN110791447A (en) * | 2019-11-21 | 2020-02-14 | 河北建设集团安装工程有限公司 | Compound microbial agent for improving water body sediment and preparation method and application thereof |
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|---|---|
| CN1189555C (en) | 2005-02-16 |
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Effective date of registration: 20040108 Address after: 226500 No. 301 dormitory building, environmental monitoring station, Jiangsu, Rugao Applicant after: Zong Xucheng Co-applicant after: Shenzhen xinruike Industrial Development Co. Ltd. Address before: 226500 No. 301 dormitory building, environmental monitoring station, Jiangsu, Rugao Applicant before: Zong Xucheng |
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