CN1421402A

CN1421402A - Microbial prepn for treating paper-making effluent and microbe culturing method

Info

Publication number: CN1421402A
Application number: CN 01139757
Authority: CN
Inventors: 李君文; 古长庆; 晁福寰; 郑金来; 王新为; 宋农
Original assignee: Institute of Hygiene and Environmental Medicine Academy of Military Medical Sciences of Chinese PLA
Current assignee: Institute of Hygiene and Environmental Medicine Academy of Military Medical Sciences of Chinese PLA
Priority date: 2001-11-30
Filing date: 2001-11-30
Publication date: 2003-06-04

Abstract

The method of treating papermaking effluent includes: separating with special culture mediums from environment the bacteria with cellulose degrading function, the bacteria with lignin degrading function, the bacteria with hemicellulose degrading function and domesticating and culturing the screened bacteria strains; preparing the cultured bacteria into liquid or solid preparation and mixing to prepare complex microbe for treating papermaking effluent; and treating papermaking effluent with the selected microbe combination while regulating the C/N2/P ratio in the aeration tank.

Description

A kind of microbial preparation and cultural method of administering paper waste

Technical field

The present invention relates to the biologic treating technique of paper waste, mainly utilize the method and the application of microbial treatment straw-pulp-papermaking wastewater.

Background technology

China is one of the most serious country of environmental pollution in the world, and the river of China, lake, ocean and soil etc. all are subjected to pollution in various degree.The annual sewage discharge in the whole nation reaches 36,000,000,000 tons, and only 10% sanitary sewage and 70% trade effluent obtain handling, and wherein have the water outlet of half industrial sewage treatment facility not reach discharging standards approximately.Other raw sewerages directly enter rivers,lakes and seas, cause the water surrounding of China to suffer severe contamination and destruction.The draining total amount of paper industry is only second to the year water displacement of chemical industry and Iron And Steel Industry, causes the severe contamination of water surrounding.The water of paper industry pollutes the focus that has become paper industry and even whole society's concern, also is the key of the papermaking enterprise survival and development.The raw material of paper industry slurrying has two kinds: timber and careless class.Be main paper making raw material with grass fiber mostly, be very main straw pulp night of administering these papermaking enterprises.Slurrying in the paper industry and bleaching process are to produce the maximum master operation of waste water.Paper-making industrial waste water mainly is made up of black digestion liquid and bleaching waste liquor, utilize microorganism and microbial enzymes to handle pulp-making waste-water and have very big advantage and potentiality, because microorganism growth and breeding very easily, enzymic catalytic reaction has the height specificity, reaction conditions gentleness, and efficient pollution-free.Adopt biological means to remove xylogen residual in the paper pulp.The structure of xylogen is very complicated, and xylogen and xylan formation complex body are difficult to remove closely attached on the fiber in paper pulp.The effect that only depends on a kind of enzyme is nowhere near, and utilizes the acting in conjunction of plurality of enzymes such as lignoenzyme to be expected to decompose fully xylogen residual in the paper pulp.In recent years, utilize the carry out a biological disposal upon research of paper waste of various lignoenzymes to rise rapidly, people expect to utilize have degraded cellulose, the bacterial strain Processing Paper Wastewater of xylogen, hemicellulose function, not only can save the cost of Processing Paper Wastewater, can also reduce secondary pollution.

The Technology of handling the paper-making industrial waste water employing at present mainly contains oxidation pond, activated sludge process, the aeration oxidation pond technology etc. of storing.The shortcoming of these methods is:

1. active sludge is formed complexity, is difficult for artificial control;

2. the inefficiency of the active sludge degraded environmental pollutant that under the nature condition, form, the effect instability;

3. floor space is big, and investment is big, and running cost is higher;

4. the active sludge treatment difficulty easily causes secondary pollution.

Summary of the invention

The purpose of this invention is to provide a kind of application of method and this method of efficient improvement paper waste.

Technical scheme of the present invention is that a kind of improvement paper waste complex microorganism and cultural method consist essentially of:

1) from environment, isolates: have the bacterium of degraded cellulose function, have and fall with various special culture medias

Separate the bacterium of lignin degrading function, have the bacterium of degradation of hemicellulose function, to separating each

Planting efficient bacterial strain tames, cultivates.Various bacterial classifications have very strong adaptability to straw-pulp-papermaking wastewater.

2) have the degraded cellulose function bacterium, have degraded lignin degrading function bacterium, have degraded half

The bacterium of Mierocrystalline cellulose function can be whiterot fungi, genus bacillus, false single-cell bacteria, wooden mould, photosynthetic thin

Microorganism such as bacterium, yeast;

3) get one or more bacterial strains and cultivate respectively with lignin degrading, Mierocrystalline cellulose and hemicellulose function,

Be processed into liquid or solid,, culture pressed different ratios mix then according to the paper waste characteristics,

Make complex microorganism;

4) according to the characteristics of paper waste, regulate the C of waste water in the aeration tank: N: P is a certain proportion, with choosing

The microorganism combination of getting is handled waste water;

Used special culture media of complex microorganism and feature thereof:

1) the used substratum composition of degraded cellulose bacterial strain with proportioning is:

Microcrystalline Cellulose 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

2) the used substratum composition of degradation of hemicellulose bacterial strain with proportioning is:

Hemicellulose 1.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

3) the used substratum composition of lignin degrading bacterial strain with proportioning is:

Xylogen 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

The present invention is used to administer paper waste, can realize the ecological relationship of hand-in-glove between the microorganism and the complementary action of Nutrition and Metabolism, and the mud behind the processing waste water can be not treated directly as organic fertilizer.Simultaneously.Processing Paper Wastewater floor space of the present invention is little, and investment and working cost are low.

Embodiment

The separation of embodiment 1, thalline, enrichment

From environment such as water, soil, bed mud, isolate: bacterium with degraded cellulose function with following various special culture medias, bacterium with lignin degrading function, bacterium with degradation of hemicellulose function, various efficient bacterial strains are tamed to separating, enrichment culture, so-called enrichment culture is meant 115 ℃ of high-temperature sterilization 15-30 of substratum minutes, the sample that to obtain in environment is under 28-37 ℃ of condition liquid culture 6-12 days earlier, line separates pure culture then, obtain pure single bacterium colony, and cultivate in a large number.Used special culture media of complex microorganism and feature thereof:

1) select, enrichment has the used substratum of degraded cellulose bacterial strain and forms with proportioning and be:

Microcrystalline Cellulose 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

2) select, enrichment has the used substratum of degradation of hemicellulose bacterial strain and forms with proportioning and be:

Hemicellulose 1.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

3) select, enrichment has the used substratum of lignin degrading bacterial strain and forms with proportioning and be:

Xylogen 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄.2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

The purification of embodiment 2, sanitary sewage and reuse:

Get certain paper waste from the paper mill, Tianjin, using microbe preparation Processing Paper Wastewater, the treatment time is 48 hours.By volume portion rate is that the mixed bacteria liquid that 2: 1: 3 ratio adds the degraded cellulose that obtains among the embodiment 1, hemicellulose, xylogen amounts to 16L in the aeration tank.Open intake pump and gas blower, continuous aeration moves sampling analysis after 2 days, the COD of its effluent quality _Cr, colourity changes and to see Table 1.As can be seen from Table 1, paper waste is after microbial preparation is handled, and COD, colourity significantly reduce.

Table 1 using microbe preparation Processing Paper Wastewater Inlet and outlet water effect comparison after 48 hours

COD (mg/L) colourity is handled preceding 760.0 355-500 and is handled back 105.2 15-30

Claims

1. a microbial preparation and a cultural method of administering paper waste consists essentially of:

1) from environment, isolates: have the bacterium of degraded cellulose function, bacterium, bacterium, tame, cultivate separating various efficient bacterial strains with degradation of hemicellulose function with degraded lignin degrading function with various special culture medias.Various bacterial classifications have very strong adaptability to straw-pulp-papermaking wastewater.

2) having the bacterium of degraded cellulose function, the bacterium with degraded lignin degrading function, bacterium with degradation of hemicellulose function can be microorganisms such as whiterot fungi, genus bacillus, false single-cell bacteria, wood are mould, photosynthetic bacterium, yeast;

3) get one or more bacterial strains and cultivate respectively, be processed into liquid or solid,, culture is pressed different ratios mix, make complex microorganism and use then according to the paper waste characteristics with lignin degrading, Mierocrystalline cellulose and hemicellulose function;

4) according to the characteristics of paper waste, regulate the carbon of waste water in the aeration tank: nitrogen: phosphorus is certain proportion, with the microorganism combination of choosing waste water is handled;

2. require used special culture media of 1 described complex microorganism and feature thereof according to patent:

Microcrystalline Cellulose 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

Hemicellulose 1.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH?7.5-8.0

3) separating the used substratum composition of xylogen bacterial strain with proportioning is:

Xylogen 0.5%

Peptone 1%

Beef powder 0.5%

KH ₂PO ₄?2H ₂O 1.0％

MgSO ₄ 0.02％

NaCl 1.0％

pH 7.5-8.0

3. it is characterized in that according to Patent right requirement 1 described microbial preparation this microbial preparation can be used for administering paper waste.

4. the method for Patent right requirement 1 can be used in combination in technological process for the treatment of papermaking with biofilm reactor.