CN102372404B - Bio-augmentation treatment sludge in-situ reduction method - Google Patents

Bio-augmentation treatment sludge in-situ reduction method Download PDF

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CN102372404B
CN102372404B CN2011102941059A CN201110294105A CN102372404B CN 102372404 B CN102372404 B CN 102372404B CN 2011102941059 A CN2011102941059 A CN 2011102941059A CN 201110294105 A CN201110294105 A CN 201110294105A CN 102372404 B CN102372404 B CN 102372404B
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sludge
original position
mud
metazoan
sewage
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CN102372404A (en
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李明智
梅荣武
喻治平
韦彦斐
张刚
王慧荣
沈浙萍
李欲如
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Zhejiang Environmental Science Research and Design Institute
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/20—Sludge processing

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  • Activated Sludge Processes (AREA)

Abstract

The invention discloses a bio-augmentation treatment sludge in-situ reduction method, which is characterized in that: on the basis of a traditional active sludge biochemical treatment system, functional microbial inoculum is additionally added to enhance organic matter degradation and a metazoan is additionally added to enhance predation so as to optimize a biotic community structure, so the sludge in-situ reduction can be realized during the sewage treatment process, and the sludge reduction effect can reach 60 percent to 100 percent. The functional microbial inoculum is a mixed flora which is specially used for the sludge reduction and is obtained through directed selection domestication culture and comprises five types of special bacterial strain selected from soil and active sludge. The metazoan is a vermes-typed micro metazoan which is selected in a laboratory and is used for the bio-augmentation predation of an active sludge system through the propagation. Through the bio-augmentation treatment method, the sludge in-situ reduction can be realized under the situation that the normal running of a sewage plant is not influenced, and the method has the characteristics of less investment, low running expense, fast action, stable running and the like. The method has general adaptability to ordinary sewage biochemical treatment systems.

Description

A kind of biological reinforced processing mud original position decrement method
Technical field
The present invention relates to the Environmental Biotechnology field, relate in particular to a kind of biological reinforced processing mud original position decrement method.
Background technology
The activated sludge process biologic process for treating sewage is widely used in China's municipal effluent and Industrial Wastewater Treatment, but effective disposal of a large amount of excess sludges of its generation also becomes a very stubborn problem.Biological process intensive treatment mud original position decrement is a kind of technology of sludge reduction cheaply, can reduce the output of excess sludge from the source, research at present mainly contains both direction: the one, reach mud decrement by adding minimizing function microbial inoculum reinforcement organic matter degradation, and the 2nd, utilize metazoan to strengthen predation and reach mud decrement.CN200810181192.5 discloses and has a kind ofly controlled and cut down the method for biological treatment of waste water system sludge output by adding special microbial inoculum, and the mud reduction can reach 10%-30%.CN200710144319.1 discloses a kind of helminth attaching biological bed device and has been used for the city sewage and sludge decrement treatment, has overcome that prior art worm adhesion amount is little, population is unstable, work capacity is low, the low defective of opposing water impact ability.CN200410009847.2 discloses a kind of deflector type oligochaetes worm sludge decrement reactor and has been used for the metazoal growth control of mud original position decrement control oligochaetes.CN201010106923.7 discloses a kind of excess sludge reduction bioreactor with variable aeration and worm attachment oblique plate, and it relates to a kind of bio-reactor that is used for excess sludge reduction.Solve existing excess sludge reduction device and had problems such as oxygen utilization rate is low, the aeration controllability is poor, worm easily comes off, the stand density of worm population is lower.CN 200910152977.4 discloses a kind of contact type tubificidae sludge decrement reactor based on the Limnodrilus hoffmeisteri effect of ingesting, and the excess sludge that the treatment plant that can be used for disposing of sewage produces is realized the purpose of mud decrement.
From disclosed patent: 1. utilize and add the mud original position Decrement Technique that microbial inoculum is strengthened organic matter degradation, though simple to operate, cost is low, effect is undesirable, and the market using value is little; 2. the mud decrement technology of at present mainly utilizing oligochaetes metazoan predation to carry out need be grown by filler (needing to add the attached type reactor), is sunken at the bottom of the pond easily, growth control is difficult, water outlet ammonia nitrogen, total phosphorus exceed standard, and process management control is had relatively high expectations, and promotes difficult.The present invention adopts the miniature metazoan of non-oligochaetes that suits in Sludge System growth of screening to prey on, and the combined function microbial inoculum is strengthened the organic matter degradation effect simultaneously, can realize the integrated of sewage disposal and mud decrement.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of biological reinforced processing mud original position decrement method is provided.
Biological reinforced processing mud original position quantitative reduction method is: functional microbial inoculum is strengthened organic matter degradation combine with the metazoan predation, realize mud original position minimizing in the sewage disposal biochemical system.
Biological reinforced processing mud original position quantitative reduction method concrete steps are as follows:
1) 5 kinds of functional bacterial strains that will screen from soil, active sludge in liquid fermentation tank, adopt common bacteria liquid nutrient medium single culture 1-2d respectively;
2) top cultured 5 kinds of bacterium liquid balanced mix are inoculated in the solid-state fermentation tank, inoculum size 20-30%, the solid medium component is: wheat bran 30-50%, corn cob meal 10-20%, dregs of beans 5-15%, starch 0.5-2%, urea 0.1-2%, Sodium Glutamate 0.5-2%, ammonium sulfate 0.2-2%, KH 2PO 40.1-0.8%, the water weight percent of regulating in the solid medium is 45-65%, and the pH value is 6.0-7.2, and temperature is 28～38 ℃ of stirring fermentations 24～48 hours of ventilating, vacuum-drying, making total nectar degree is 10 8-14The solid fungicide of CFU/ml;
3) the miniature metazoan of the rotifers that will screen from mud joins in the mixed solution of active sludge and nutritive medium and carries out enlarged culturing, regularly adds nutritive medium, cultivates 7-20d, obtains the muddy water mixed solution that polypide density is 150-800 bar/ml;
4) with step 2) solid fungicide made is dissolved in the sterilized water that 5-10 doubly measures, aeration activation 1-3d, be added in the biochemical system with peristaltic pump stream, simultaneously the miniature metazoan muddy water mixed solution of the rotifers after the step 3) enlarged culturing be added to biochemical system and strengthen predation.
Described common bacteria liquid nutrient medium is beef-protein medium, LB liquid nutrient medium or YPD substratum.
Described nutritive medium is formulated in the 100:1-10:0.5-3.5:0.5-2.5:1-5.5:0.1-1.5 ratio by carbon, nitrogen, phosphorus, potassium, calcium, magnesium elements.
Biological reinforced processing mud original position quantitative reduction method is applied to activated sludge process, biomembrance process biologic process for treating sewage.
Described sewage is sanitary sewage and the pretreated trade effluent of process.
The beneficial effect that the present invention compared with prior art has:
(1) miniature metazoan growth is digested excess sludge with the microbial inoculum intensifying technology is combined, can in biochemical system, realize the mud decrement effect of 60-100%;
(2) can under additional equipment situation not, realize the original position minimizing of wastewater treatment biochemical system excess sludge, bio-chemical effluent water quality is not had detrimentally affect substantially;
(3) can under the situation that does not influence the normal operation of Sewage Plant, implement biological reinforced sludge reduction transformation, reduced investment, low, the instant effect of working cost, application value height.
Embodiment
Biological reinforced processing mud original position quantitative reduction method is: functional microbial inoculum is strengthened organic matter degradation combine with the metazoan predation, realize mud original position minimizing in the sewage disposal biochemical system.
Biological reinforced processing mud original position quantitative reduction method concrete steps are as follows:
1) 5 kinds of functional bacterial strains that will screen from soil, active sludge in liquid fermentation tank, adopt common bacteria liquid nutrient medium single culture 1-2d respectively;
2) top cultured 5 kinds of bacterium liquid balanced mix are inoculated in the solid-state fermentation tank, inoculum size 20-30%, the solid medium component is: wheat bran 30-50%, corn cob meal 10-20%, dregs of beans 5-15%, starch 0.5-2%, urea 0.1-2%, Sodium Glutamate 0.5-2%, ammonium sulfate 0.2-2%, KH 2PO 40.1-0.8%, the water weight percent of regulating in the solid medium is 45-65%, and the pH value is 6.0-7.2, and temperature is 28～38 ℃ of stirring fermentations 24～48 hours of ventilating, vacuum-drying, making total nectar degree is 10 8-14The solid fungicide of CFU/ml;
3) the miniature metazoan of the rotifers that will screen from mud joins in the mixed solution of active sludge and nutritive medium and carries out enlarged culturing, regularly adds nutritive medium, cultivates 7-20d, obtains the muddy water mixed solution that polypide density is 150-800 bar/ml;
4) with step 2) solid fungicide made is dissolved in the sterilized water that 5-10 doubly measures, aeration activation 1-3d, be added in the biochemical system with peristaltic pump stream, simultaneously the miniature metazoan muddy water mixed solution of the rotifers after the step 3) enlarged culturing be added to biochemical system and strengthen predation.
Described common bacteria liquid nutrient medium is beef-protein medium, LB liquid nutrient medium or YPD substratum.
Described nutritive medium is formulated in the 100:1-10:0.5-3.5:0.5-2.5:1-5.5:0.1-1.5 ratio by carbon, nitrogen, phosphorus, potassium, calcium, magnesium elements.
Biological reinforced processing mud original position quantitative reduction method is applied to activated sludge process, biomembrance process biologic process for treating sewage.
Described sewage is sanitary sewage and the pretreated trade effluent of process.
Embodiment 1
Adopt beef-protein medium, prescription is extractum carnis 3g, peptone 10g, Nacl5g, water 1000mL, pH7.4～7.6,5 kinds of bacterial strains of yeast, genus bacillus, pseudomonas, photosynthetic bacterium, micrococci single culture in the 10L fermentor tank that screening is obtained, incubation time 48h, each obtains bacterium liquid 6L; Top cultured 30L bacterium mixed solution is inoculated in the 100L solid-state fermentation tank, inoculum size 30%, the solid culture based component is a wheat bran 50%, corn cob meal 10%, dregs of beans 15%, starch 2%, urea 2%, Sodium Glutamate 0.5%, ammonium sulfate 2%, KH 2PO 40.8%, adding water adjusting weight in wet base per-cent is 45%, regulates PH 7.2, and temperature is ventilated for 36 ℃ and stirred fermentation after 48 hours, makes solid fungicide again after vacuum-drying; Cultivating detection live bacteria number through the agar coated plate is 10 14CFU/g.
Embodiment 2
Adopt the LB liquid nutrient medium, filling a prescription is peptone 10g/L, yeast extract 5g/L, and sodium-chlor 10g/L, with 5 class bacterial strains single culture in the 10L fermentor tank, incubation time 24h, each obtains bacterium liquid 4L; Top cultured 20L bacterium mixed solution is inoculated in the 100L solid-state fermentation tank, inoculum size 20%, the solid culture based component is a wheat bran 30%, corn cob meal 20%, dregs of beans 5%, starch 0.5%, urea 0.1%, Sodium Glutamate 2%, ammonium sulfate 0.2%, KH 2PO 40.1%, adding water adjusting weight in wet base per-cent is 65%, regulates PH 6.0, and temperature is ventilated for 28 ℃ and stirred fermentation after 24 hours, makes solid fungicide again after vacuum-drying; Cultivating detection live bacteria number through the plain agar coated plate is 10 9CFU/g.
Embodiment 3
Method is used for the mud original position minimizing of city domestic sewage activated sludge process second-stage treatment, adopts hypoxic/anaerobic/aerobic (AO/A 2O) technology, influent quality average COD 340 mg/L, BOD 155 mg/L, NH3-N 30 mg/L, TN 41 mg/L, TP 4.5 mg/L, SS 107 mg/L, the water yield 2.5 ten thousand t/d, total hrt 14 h.Former technology effluent quality average COD 32 mg/L, BOD 7.6 mg/L, NH3-N 5 mg/L, TN 13 mg/L, TP 0.4 mg/L, SS 8 mg/L produce total surplus sludge quantity 5.5t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 10 ppm, and after Continuous Flow added 2 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously the miniature metazoan of cultured rotifers was added in the Aerobic Pond, and dosage is that effective pond holds 1/10 5, strengthen second pond mud quantity of reflux, keep not spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 1500-3000mg/L, this moment, biochemical system did not have excess sludge to produce substantially.Effluent quality keeps original state of the art substantially, average COD 31 mg/L, BOD 8 mg/L, NH3-N 4.6 mg/L, TN 12mg/L, TP 0.45 mg/L, SS 8 mg/L, the water outlet leading indicator reaches urban wastewater treatment firm one-level A standard, biochemical system excess sludge generation 0 t/d, only have preliminary sedimentation tank to produce mud 0. 4 t/d in the Sewage treatment systems, the mud decrement effect reaches more than 90%.
Embodiment 4
Method is used for the mud original position minimizing of city domestic sewage activated sludge process second-stage treatment, adopt the alternative expression oxidation ditch process, influent quality COD 260 mg/L, BOD 124 mg/L, NH3-N 25 mg/L, TN 32 mg/L, TP 3.5 mg/L, SS 76 mg/L, the water yield 30,000 t/d, total hrt 11 h.Former technology effluent quality average COD 26 mg/L, BOD 6.5 mg/L, NH3-N 4.4 mg/L, TN11 mg/L, TP 0.4 mg/L, SS 6 mg/L produce surplus sludge volume 6 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 10 times of sterilized water aeration activation 3d, with peristaltic pump respectively stream be added to the aerobic section water-in, the stream dosage is 10 ppm, and after Continuous Flow added 2 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 5/10 6, strengthen second pond mud quantity of reflux, keep not spoil disposal of biochemical system, move 3w continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 1500-4000mg/L, this moment, biochemical system was substantially without spoil disposal.Effluent quality keeps original state of the art, average COD 28 mg/L, BOD 7 mg/L, NH3-N 4.2 mg/L, TN 10 mg/L, TP 0.38 mg/L, SS 6.5 mg/L, the water outlet leading indicator reaches urban wastewater treatment firm one-level A standard, biochemical system excess sludge generation 0 t/d, only have preliminary sedimentation tank to produce mud 0. 3t/d in the Sewage treatment systems, the mud decrement effect reaches more than 95%.
Embodiment 5
Method is used for the mud original position minimizing of city domestic sewage activated sludge process second-stage treatment, adopt SBR technology, waste water contains 30% and receives plumber industry waste water, influent quality average COD 410 mg/L, BOD 165 mg/L, NH3-N 51 mg/L, TN 68 mg/L, TP 8.2 mg/L, SS 121 mg/L, the water yield 1.1 ten thousand t/d, total hrt 16 h.Former technology effluent quality average COD 55 mg/L, BOD 13 mg/L, NH3-N 6.5mg/L, TN 15 mg/L, TP 0.9 mg/L, SS 16 mg/L produce surplus sludge volume 5 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 15 ppm, and after Continuous Flow added 2 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 2/10 5, strengthen second pond mud quantity of reflux, keep a small amount of spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 2000-4000mg/L, a small amount of spoil disposal of biochemical system this moment.Effluent quality keeps original state of the art substantially, average COD 51 mg/L, BOD 12 mg/L, NH3-N 7mg/L, TN 16 mg/L, TP 0.8 mg/L, SS 18 mg/L, the water outlet leading indicator reaches urban wastewater treatment firm one-level B standard, biochemical system excess sludge generation 0 t/d, preliminary sedimentation tank produces mud 0. 5t/d, and the mud decrement effect reaches more than 85%.
Embodiment 6
Method is used for the mud original position minimizing of city domestic sewage biomembrance process second-stage treatment, adopt SBR technology, waste water contains 40% and receives plumber industry waste water, influent quality average COD 680 mg/L, BOD 245 mg/L, NH3-N 62 mg/L, TN 77 mg/L, TP 9.1 mg/L, SS 152 mg/L, the water yield 0.8 ten thousand t/d, total hrt 18 h.Former technology effluent quality average COD 56 mg/L, BOD 16 mg/L, NH3-N 7.5mg/L, TN 10 mg/L, TP 0.9 mg/L, SS 14 mg/L produce surplus sludge volume 4 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 10 ppm, and after Continuous Flow added 2 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 1/10 5, strengthen the mud quantity of reflux, keep a small amount of spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 2000-4000mg/L, biochemical system this moment spoil disposal not substantially.Effluent quality keeps original state of the art substantially, average COD 48 mg/L, BOD 10 mg/L, NH3-N 6.5 mg/L, TN 14 mg/L, TP 0.65 mg/L, SS 8 mg/L, the water outlet leading indicator reaches urban wastewater treatment firm one-level B standard, biochemical system excess sludge generation 0.4 t/d, preliminary sedimentation tank produces mud 0.2 t/d, and the mud decrement effect reaches more than 80%.
Embodiment 7
Method is used for the mud original position minimizing of industrial sewage activated sludge process second-stage treatment, the water inlet composition is mainly printing and dyeing class waste water, adopt hydrolysis+anaerobism+aerobic process, total hrt 21h, influent quality average COD 1500 mg/L, BOD 360 mg/L, NH3-N 45 mg/L, TN 60 mg/L, TP 7.5 mg/L, SS 176 mg/L, the water yield 1.4 ten thousand t/d.Former technology effluent quality average COD 360 mg/L, BOD 32 mg/L, NH3-N 15mg/L, TN 22 mg/L, TP 1.1 mg/L, SS 18 mg/L produce surplus sludge volume 16 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 15 ppm, and after Continuous Flow added 2 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 2/10 5, strengthen second pond mud quantity of reflux, keep a small amount of spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 2000-4000mg/L, a small amount of spoil disposal of biochemical system this moment.Effluent quality is better than original state of the art, average COD 268 mg/L, BOD 24 mg/L, NH3-N 10mg/L, TN 19 mg/L, TP 0.95 mg/L, SS 12 mg/L, biochemical system excess sludge generation 1 t/d, preliminary sedimentation tank produces mud 0. 8 t/d, and the mud decrement effect reaches more than 80%.
Embodiment 8
Method is used for the mud original position minimizing of industrial sewage active bio embrane method second-stage treatment, water inlet is mainly the pharmaceutical trade effluent, adopt hydrolysis+anaerobism+aerobic process, total hrt 24h, influent quality average COD 2600 mg/L, BOD 550 mg/L, NH3-N 265 mg/L, TN 320 mg/L, TP 28 mg/L, SS 220 mg/L, the water yield 1.6 ten thousand t/d.Former technology effluent quality average COD 450 mg/L, BOD 58 mg/L, NH3-N 25mg/L, TN 42 mg/L, TP 2.1 mg/L, SS 26 mg/L produce surplus sludge volume 31 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 20 ppm, and after Continuous Flow added 3 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 2/10 5, strengthen second pond mud quantity of reflux, keep a small amount of spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 2000-4000mg/L, a small amount of spoil disposal of biochemical system this moment.Effluent quality is better than original state of the art, average COD 420 mg/L, BOD 52 mg/L, NH3-N 15 mg/L, TN 36 mg/L, TP 1.65 mg/L, SS 16 mg/L, biochemical system excess sludge generation 6.5t/d, preliminary sedimentation tank produces mud 1. 5t/d, and the mud decrement effect reaches more than 70%.
Embodiment 9
Method is used for the mud original position minimizing of the active activated sludge process second-stage treatment of industrial sewage, water inlet is mainly the foodstuff trade effluent, adopt hydrolysis+anaerobism+aerobic process, total hrt 32h, influent quality average COD 5800 mg/L, BOD 2250 mg/L, NH3-N 125 mg/L, TN 320 mg/L, TP 55 mg/L, SS 580 mg/L, the water yield 10,000 t/d.Former technology effluent quality average COD 120 mg/L, BOD 28 mg/L, NH3-N 20mg/L, TN 36 mg/L, TP 1.2 mg/L, SS 21 mg/L produce surplus sludge volume 35 t/d(through filter press, 85% water content).Do not change former technology and carry out biological reinforced processing, earlier functional microbial inoculum is added 5 times of sterilized water aeration activation 2d, with peristaltic pump respectively stream be added to anoxic water-in and aerobic water-in, the stream dosage is 20 ppm, and after Continuous Flow added 4 w, sludge character obviously improved, the mud generation begins to reduce, this moment, but the gap added microbial inoculum, simultaneously cultured miniature metazoan was added in the Aerobic Pond, and dosage is that effective pond holds 1/10 5, strengthen second pond mud quantity of reflux, keep a small amount of spoil disposal of biochemical system, move 1 month continuously after, the biochemical system sludge concentration progressively descends, and finally is stabilized in 2000-4000mg/L, a small amount of spoil disposal of biochemical system this moment.Effluent quality is better than original state of the art, average COD 100 mg/L, BOD 22 mg/L, NH3-N 12 mg/L, TN 24 mg/L, TP 0.85 mg/L, SS 18 mg/L, biochemical system excess sludge generation 3.5 t/d, preliminary sedimentation tank produces mud 2. 2 t/d, and the mud decrement effect reaches more than 80%.
Embodiment 10
5 kinds of functional bacterial strains that will screen from soil, active sludge in liquid fermentation tank, adopt beef-protein medium single culture 1d respectively; Top cultured 5 kinds of bacterium liquid balanced mix are inoculated in the solid-state fermentation tank, inoculum size 20%, the solid medium component is: wheat bran 30%, corn cob meal 10%, dregs of beans 5%, starch 0.5%, urea 0.1%, Sodium Glutamate 0.5%, ammonium sulfate 0.2%, KH 2PO 40.1%, the water weight percent of regulating in the solid medium is 45%, and the pH value is 6.0, and temperature is 28 ℃ of stirring fermentations 24 hours of ventilating, vacuum-drying, and making total nectar degree is 10 8-14The solid fungicide of CFU/ml; The miniature metazoan of the rotifers that will screen from mud joins in the mixed solution of active sludge and nutritive medium and carries out enlarged culturing, regularly adds nutritive medium, cultivates 7d, obtains the muddy water mixed solution that polypide density is 150/ml; Solid fungicide is dissolved in the sterilized water of 5 times of amounts, aeration activation 1d is added in the biochemical system with peristaltic pump stream, simultaneously the miniature metazoan muddy water mixed solution of the rotifers after the enlarged culturing is added to biochemical system and strengthens predation.Described nutritive medium is formulated in the 100:1:0.5:0.5:1:0.1 ratio by carbon, nitrogen, phosphorus, potassium, calcium, magnesium elements.
Embodiment 11
5 kinds of functional bacterial strains that will screen from soil, active sludge in liquid fermentation tank, adopt LB liquid nutrient medium single culture 2d respectively; Top cultured 5 kinds of bacterium liquid balanced mix are inoculated in the solid-state fermentation tank, inoculum size 30%, the solid medium component is: wheat bran 50%, corn cob meal 20%, dregs of beans 15%, starch 2%, urea 2%, Sodium Glutamate 2%, ammonium sulfate 2%, KH 2PO 40.8%, the water weight percent of regulating in the solid medium is 65%, and the pH value is 7.2, and temperature is 38 ℃ of stirring fermentations 48 hours of ventilating, vacuum-drying, and making total nectar degree is 10 8-14The solid fungicide of CFU/ml; The miniature metazoan of the rotifers that will screen from mud joins in the mixed solution of active sludge and nutritive medium and carries out enlarged culturing, regularly adds nutritive medium, cultivates 20d, obtains the muddy water mixed solution that polypide density is 800/ml; Solid fungicide is dissolved in the sterilized water of 10 times of amounts, aeration activation 3d is added in the biochemical system with peristaltic pump stream, simultaneously the miniature metazoan muddy water mixed solution of the rotifers after the enlarged culturing is added to biochemical system and strengthens predation.Described nutritive medium is formulated in 100:10:3.5:2.5:5.5:0. 1.5 ratios by carbon, nitrogen, phosphorus, potassium, calcium, magnesium elements.

Claims (5)

1. a biological reinforced processing mud original position quantitative reduction method is characterized in that functional microbial inoculum is strengthened organic matter degradation to combine with the metazoan predation, realizes mud original position minimizing in the sewage disposal biochemical system; Concrete steps are as follows:
1) yeast that will screen from soil, active sludge, genus bacillus, pseudomonas, photosynthetic bacterium, 5 kinds of bacterial strains of micrococci in liquid fermentation tank, adopt bacterial liquid substratum single culture 1-2d respectively;
2) top cultured 5 kinds of bacterium liquid balanced mix are inoculated in the solid-state fermentation tank, inoculum size 20-30%, the solid medium component is: wheat bran 30-50%, corn cob meal 10-20 %, dregs of beans 5-15%, starch 0.5-2%, urea 0.1-2%, Sodium Glutamate 0.5-2%, ammonium sulfate 0.2-2%, KH 2PO 40.1-0.8%, the water weight percent of regulating in the solid medium is 45-65%, and the pH value is 6.0-7.2, and temperature is 28～38 ℃ of stirring fermentations 24～48 hours of ventilating, vacuum-drying, making total nectar degree is 10 8-14The solid fungicide of CFU/ml;
3) the miniature metazoan of the rotifers that will screen from mud joins in the mixed solution of active sludge and nutritive medium and carries out enlarged culturing, regularly adds nutritive medium, cultivates 7-20d, obtains the muddy water mixed solution that polypide density is 150-800 bar/ml;
4) with step 2) solid fungicide made is dissolved in the sterilized water that 5-10 doubly measures, aeration activation 1-3d, be added in the biochemical system with peristaltic pump stream, simultaneously the miniature metazoan muddy water mixed solution of the rotifers after the step 3) enlarged culturing be added to biochemical system and strengthen predation.
2. a kind of biological reinforced processing mud original position quantitative reduction method according to claim 1 is characterized in that described bacterial liquid substratum is beef-protein medium, LB liquid nutrient medium or YPD substratum.
3. a kind of biological reinforced processing mud original position quantitative reduction method according to claim 1, it is characterized in that described nutritive medium is formulated in the 100:1-10:0.5-3.5:0.5-2.5:1-5.5:0.1-1.5 ratio by carbon, nitrogen, phosphorus, potassium, calcium, magnesium elements.
4. the application of a biological reinforced processing mud original position quantitative reduction method as claimed in claim 1 is characterized in that, is applied to activated sludge process, biomembrance process biologic process for treating sewage.
5. the application of a kind of biological reinforced processing mud original position quantitative reduction method according to claim 4 is characterized in that, described sewage is sanitary sewage and the pretreated trade effluent of process.
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