CN113816498B - Method for improving biological treatment efficiency of wastewater by adding bdelloid rotifers - Google Patents
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- 241000700141 Rotifera Species 0.000 title claims abstract description 95
- 239000002351 wastewater Substances 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 42
- 239000010802 sludge Substances 0.000 claims abstract description 24
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 19
- 229930182490 saponin Natural products 0.000 claims abstract description 19
- 150000007949 saponins Chemical class 0.000 claims abstract description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 240000008042 Zea mays Species 0.000 claims abstract description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 15
- 235000005822 corn Nutrition 0.000 claims abstract description 15
- 235000013312 flour Nutrition 0.000 claims abstract description 15
- 239000011780 sodium chloride Substances 0.000 claims abstract description 9
- 241000604933 Bdellovibrio Species 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 5
- 239000007787 solid Substances 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 230000000644 propagated effect Effects 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 241000237677 Hirudinaria Species 0.000 abstract 2
- 235000013339 cereals Nutrition 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 21
- 238000004939 coking Methods 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000004043 dyeing Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000244174 Strongyloides Species 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Water Supply & Treatment (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
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Abstract
The invention discloses a method for improving biological treatment efficiency of wastewater by adding a bdelloid rotifer, which comprises the following steps: 1) The Bdellovibrio separated from the activated sludge is 0.05 percent to over-headRepeatedly cleaning in 0.2% sodium chloride solution, and then manually culturing at 20-30 ℃; 2) The food for culturing the bdelloid rotifers is corn flour with the grain diameter smaller than 2 mu m obtained by sieving; 3) Adding the bdelloid rotifer culture solution into the reactor once or adding once again every other week, wherein the adding density is 100-5000 ind/m 3 The method comprises the steps of carrying out a first treatment on the surface of the 4) Adding the hirudinaria with the concentration of 10-100 mug/L plant saponin to promote the reproduction of the hirudinaria. The invention provides a method for improving biological treatment efficiency of wastewater, which has low cost, easy implementation and ecology.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a method for improving biological wastewater treatment efficiency by adding a bdelloid rotifer.
Background
Compared with the physicochemical treatment method, the biological treatment method for the wastewater by utilizing microorganisms such as bacteria, fungi and the like has low cost and environmental friendliness, but has low treatment efficiency on organic wastewater which is difficult to degrade.
In a wastewater biological treatment system, there are micro-animals such as protozoa and micro-metazoan in addition to microorganisms. Bdellovely rotifers in micro-animals are a common class of organisms beneficial to biological treatment, and their beneficial effects include promotion of flocculation of sludge, improvement of organic matter removal and nitrification rates, reduction of excess sludge, and the like. Chinese patent (Zl 2016610119990. X) discloses a method for promoting the growth of flocculating bacteria using a culture of gyrophora. Chinese patent (CN 101432233 a) discloses a method for improving the treatment efficiency of activated sludge process and reducing the amount of excess sludge produced by utilizing predation of micro-animals;
the use of micro-animals to increase the biological wastewater treatment efficiency is an ecologically friendly method, but how to enable specific micro-animals to exist and reproduce in a wastewater biological treatment reactor is a technical problem to be solved. In view of the above, the technical problem to be solved by the invention is to provide a method for improving the biological treatment efficiency of waste water by using the bdelloid rotifer and improving the survival and reproduction capability of the bdelloid rotifer in the biological treatment reactor of the waste water.
Disclosure of Invention
The invention aims to provide a method for improving biological treatment efficiency of wastewater by adding bdelloid rotifers, so as to solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for improving biological treatment efficiency of wastewater by adding a bdelloid rotifer comprises the following steps:
A. separating and purifying the bdelloid rotifers;
B. culturing the bdelloid rotifers;
C. adding a bdelloid rotifer;
D. the bdelloid rotifers are bred and propagated;
the specific method in the step A is as follows: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking out a bdelloid wheel from the sample in the culture dish by a pipette gun under the magnification of 10-20 times, transferring the bdelloid wheel into the culture dish containing 0.05-0.2% sodium chloride solution, and shaking the culture dish for 15-30 times; placing the culture dish under a solid microscope, sucking out the bdelloid rotifers from the culture dish solution by using a pipette gun, transferring the rotifers into other culture dishes containing 0.05-0.2% sodium chloride solution, and shaking the culture dish for 15-30 times; repeating the above process for 4 times to obtain a bdelloid wheel without other micro animals;
the specific method in the step B is as follows: sieving corn flour, collecting corn flour granule with particle diameter less than 2 μm as food for Bdellovibrio, and the density of corn flour granule is 1×10 4 ～1×10 8 The particles/mL are put into a culture solution with the pH value of 6 to 8 for culture, and aeration is carried out on the culture solution to keep the dissolved oxygen at 1 to 5mg/LAseptically culturing at 20-30 ℃;
the specific method in the step C is as follows: the cultured bdelloid rotifer is firstly added into the wastewater biological treatment reactor, and the adding density of the bdelloid rotifer is 100-5000 ind/m 3 After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first fed bdelloid rotifers, so as to maintain the number of the bdelloid rotifers in the reactor;
the specific method in the step D is as follows: adding plant-source saponin with the concentration of 10-100 mug/L into the waste water bioreactor when adding the bdelloviform rotifer, and adding the plant-source saponin again at intervals of 3-7 weeks, and continuously adding for 4 times at intervals;
the bdelloid rotifer is one or more of rotifer, strongyloides, etc. commonly found in biological treatment.
Compared with the prior art, the invention has the beneficial effects that: the invention has the remarkable advantages that: 1) The bdelloid rotifer is from a wastewater biological treatment reactor and has genetic characteristics suitable for wastewater biological treatment environment; 2) The corn flour used as the food of the bdelloid rotifers has the characteristics of wide material source and low price; 3) The bdelloid rotifer culture solution contains not only the bdelloid rotifer metabolite with the function of promoting the bacterial activity, but also organic matters such as polysaccharide and the like which can play a role of co-metabolism so as to be beneficial to improving the biological treatment efficiency; 4) Directly inputting a culture solution containing the bdelloid rotifers into the wastewater biological treatment device, and separating the rotifers from the rotifer culture solution is not needed, so that the operation steps are simplified; 5) The saponin with biological activity is added while the bdelloid rotifer is added into the biological treatment reactor, so that the survival and reproductive capacity of the bdelloid rotifer are improved, and the bdelloid rotifer is helped to play a role in improving the biological treatment efficiency of the wastewater.
Drawings
FIG. 1 is a graph showing the COD removal rate of printing and dyeing wastewater when Bdellovely rotifer is added in the embodiment of the invention;
FIG. 2 is a graph showing the effect of COD removal rate of coking wastewater when Bdellovibrio is added in the second embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiment one:
in this embodiment, referring to fig. 1, the method for improving the biological treatment efficiency of the printing and dyeing wastewater by using the bdelloid rotifers is performed according to the following steps:
the leech-shaped wheel worm separation method comprises the following steps: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking out a bdelloid wheel from the sample in the culture dish by a pipette gun under the magnification of 15 times, transferring the bdelloid wheel into the culture dish containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; placing the culture dish under a solid microscope, sucking out the bdelloid rotifers from the culture dish solution by using a pipette gun, transferring the rotifers into other culture dishes containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; repeating the above process for 4 times to obtain a bdelloid wheel without other micro animals;
the bdelloid rotifer culturing method comprises the following steps: sieving corn flour, collecting corn flour granule with particle diameter of 1 μm as food for Bdellovibrio, and the density of corn flour granule is 1×10 6 The Bdellovibrio is placed in a culture solution with the pH value of 7 for culture and is placed in a condition of 25 ℃ for sterile culture;
the leech-shaped wheel worm adding method comprises the following steps: the cultured bdelloid rotifer is firstly added into the wastewater biological treatment reactor, and the adding density of the bdelloid rotifer is 2000ind/m 3 After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first fed bdelloid rotifers, so as to maintain the number of the bdelloid rotifers in the reactor;
D. the breeding and reproducing method of the bdelloid rotifers comprises the following steps: plant-derived saponin with a concentration of 50 mug/L was added to the wastewater bioreactor, and plant-derived saponin was added again at 4 weeks intervals, and the addition was continued 4 times at this time interval.
Bdellovely rotifer promotes the biological treatment efficiency analysis of printing and dyeing wastewater:
as can be seen from FIG. 1, 3g/L of activated sludge (group A) and 500ind/m of activated sludge were added to the printing and dyeing wastewater 3 Bdellovely rotifer (group B) added with 3g/L activated sludge, 500ind/m 3 The method comprises the steps of adding the cultured bdelloid rotifer once after every other week, adding the plant saponin with the concentration of 50 mug/L after adding the bdelloid rotifer for 1 day, adding the plant saponin again at intervals of 5 days, and continuously adding for 4 times. Compared with the control group without Bdellovely rotifer and saponin, the COD removal rate of the B group is 83.1%, the COD removal rate of the C group is 90.3%, and the COD removal rate of the A group is 72.7%. The bdelloviform wheel worm has a certain promotion effect on the biological treatment efficiency of the printing and dyeing wastewater, and the saponin has the effect of obviously improving the biological activity, so that the treatment efficiency is higher.
Embodiment two:
this embodiment is substantially the same as the first embodiment, and is characterized in that:
in this example, referring to fig. 2, the method for improving biological treatment efficiency of coking wastewater by using bdelloid rotifers is performed according to the following steps:
the leech-shaped wheel worm separation method comprises the following steps: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking out a bdelloid wheel from the sample in the culture dish by a pipette gun under the magnification of 15 times, transferring the bdelloid wheel into the culture dish containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; placing the culture dish under a solid microscope, sucking out the bdelloid rotifers from the culture dish solution by using a pipette gun, transferring the rotifers into other culture dishes containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; repeating the above process for 4 times to obtain a bdelloid wheel without other micro animals;
the bdelloid rotifer culturing method comprises the following steps: sieving corn flour, taking corn flour particles with particle size of 1 μm as food of Bdellovibrio, and collecting corn flour particlesIs 1 x 10 in density 6 The Bdellovibrio is placed in a culture solution with the pH value of 7 for culture and is placed in a condition of 25 ℃ for sterile culture;
the leech-shaped wheel worm adding method comprises the following steps: the cultured bdelloid rotifer is firstly added into the wastewater biological treatment reactor, and the adding density of the bdelloid rotifer is 2000ind/m 3 After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first fed bdelloid rotifers, so as to maintain the number of the bdelloid rotifers in the reactor;
the breeding method for improving the breeding and reproduction of the bdelloviform rotifer comprises the following steps: plant-derived saponin with a concentration of 50 mug/L was added to the wastewater bioreactor, and plant-derived saponin was added again at 4 weeks intervals, and the addition was continued 4 times at this time interval.
Preliminary dilution is carried out to regulate COD of coking wastewater, 3g/L of activated sludge is added in group A, 3g/L of activated sludge and 500ind/m of activated sludge are added in group B 3 Bdellovely rotifer of (C group) added with 3g/L activated sludge, 500ind/m 3 The method comprises the steps of adding the cultured bdelloid rotifer once after every other week, adding the plant saponin with the concentration of 50 mug/L after adding the bdelloid rotifer for 1 day, adding the plant saponin again at intervals of 5 days, and continuously adding for 4 times.
The supernatant was collected and its COD was measured to obtain three sets of treated coking wastewater COD comparisons as shown in FIG. two. Analysis of biological treatment efficiency of coking wastewater promoted by rotifer culture solution:
as is clear from fig. 2, the COD removal rate of group B was 77.3%, the COD removal rate of group C was 83.6%, and the COD removal rate of group a was 62.1%, as compared with the control group to which no bdellovid and saponin were added. The bdelloviform wheel worm has a certain promotion effect on the biological treatment efficiency of the coking wastewater, and under the same microorganism and micro-animal treatment conditions, the saponin has the effect of obviously improving the biological activity, and the COD removal efficiency is higher.
In summary, the invention has the remarkable advantages that: 1) The bdelloid rotifer is from a wastewater biological treatment reactor and has genetic characteristics suitable for wastewater biological treatment environment; 2) The corn flour used as the food of the bdelloid rotifers has the characteristics of wide material source and low price; 3) The bdelloid rotifer culture solution contains not only the bdelloid rotifer metabolite with the function of promoting the bacterial activity, but also organic matters such as polysaccharide and the like which can play a role of co-metabolism so as to be beneficial to improving the biological treatment efficiency; 4) Directly inputting a culture solution containing the bdelloid rotifers into the wastewater biological treatment device, and separating the rotifers from the rotifer culture solution is not needed, so that the operation steps are simplified; 5) The saponin with biological activity is added while the bdelloid rotifer is added into the biological treatment reactor, so that the survival and reproductive capacity of the bdelloid rotifer are improved, and the bdelloid rotifer is helped to play a role in improving the biological treatment efficiency of the wastewater. It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (1)
1. A method for improving biological treatment efficiency of wastewater by adding a bdelloid rotifer is characterized by comprising the following steps: the method comprises the following steps:
A. separating and purifying the bdelloid rotifers;
B. culturing the bdelloid rotifers;
C. adding a bdelloid rotifer;
D. the bdelloid rotifers are bred and propagated;
the specific method in the step A is as follows: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking out a bdelloid wheel from the sample in the culture dish by a pipette gun under the magnification of 10-20 times, transferring the bdelloid wheel into the culture dish containing 0.05-0.2% sodium chloride solution, and shaking the culture dish for 15-30 times; placing the culture dish under a solid microscope, sucking out the bdelloid rotifers from the culture dish solution by using a pipette gun, transferring the rotifers into other culture dishes containing 0.05-0.2% sodium chloride solution, and shaking the culture dish for 15-30 times; repeating the above process for 4 times to obtain a bdelloid wheel without other micro animals;
the specific method in the step B is as follows: sieving corn flour, collecting corn flour granule with particle diameter less than 2 μm as food for Bdellovibrio, and the density of corn flour granule is 1×10 4 ～1×10 8 The Bdellovely wheel worm is placed in a culture solution with the pH value of 6 to 8 for culture, aeration is carried out to the culture solution to keep the dissolved oxygen at 1 to 5mg/L, and the culture solution is placed in a 20 to 30 ℃ condition for sterile culture;
the specific method in the step C is as follows: the cultured bdelloid rotifer is firstly added into the wastewater biological treatment reactor, and the adding density of the bdelloid rotifer is 100-5000 ind/m 3 After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first fed bdelloid rotifers, so as to maintain the number of the bdelloid rotifers in the reactor;
the specific method in the step D is as follows: adding plant-source saponin with the concentration of 10-100 mug/L into the waste water bioreactor when adding the bdelloviform rotifer, and adding the plant-source saponin again at intervals of 3-7 weeks, and continuously adding for 4 times at intervals;
the bdelloid rotifer is one or more of rotifer and rotifer commonly used in biological treatment.
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