CN113816498A - Method for improving biological treatment efficiency of wastewater by adding hirudo - Google Patents
Method for improving biological treatment efficiency of wastewater by adding hirudo Download PDFInfo
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- CN113816498A CN113816498A CN202111276433.6A CN202111276433A CN113816498A CN 113816498 A CN113816498 A CN 113816498A CN 202111276433 A CN202111276433 A CN 202111276433A CN 113816498 A CN113816498 A CN 113816498A
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- 239000002351 wastewater Substances 0.000 title claims abstract description 45
- 241000237903 Hirudo Species 0.000 title claims abstract description 4
- 241000700141 Rotifera Species 0.000 claims abstract description 119
- 239000010802 sludge Substances 0.000 claims abstract description 23
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 22
- 229930182490 saponin Natural products 0.000 claims abstract description 22
- 150000007949 saponins Chemical class 0.000 claims abstract description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 16
- 240000008042 Zea mays Species 0.000 claims abstract description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 15
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- 238000012258 culturing Methods 0.000 claims abstract description 7
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- 108010055460 bivalirudin Proteins 0.000 claims abstract description 5
- OIRCOABEOLEUMC-GEJPAHFPSA-N bivalirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 OIRCOABEOLEUMC-GEJPAHFPSA-N 0.000 claims abstract description 5
- 229960001500 bivalirudin Drugs 0.000 claims abstract description 5
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- 238000009395 breeding Methods 0.000 claims description 6
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- 239000002245 particle Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
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- 241001494858 Habrotrocha Species 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000004065 wastewater treatment Methods 0.000 abstract description 7
- 235000013339 cereals Nutrition 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 8
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- 230000000813 microbial effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Water Supply & Treatment (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for improving biological wastewater treatment efficiency by adding hirudo rotifers, which comprises the following steps: 1) the method comprises the following steps of (1) repeatedly cleaning leech-shaped rotifers separated from activated sludge in 0.05-0.2% sodium chloride solution, and then carrying out artificial culture at 20-30 ℃; 2) the food for culturing the hirulog rotifer is corn flour with the grain size of less than 2 mu m obtained by screening; 3) adding a bdelloid rotifer culture solution into the reactor once or adding the culture solution once every other week, wherein the adding density is 100-5000 ind/m3(ii) a 4) Adding 10-100 mu g/L of plant saponin after adding the bdelloid rotifers to promote the reproduction of the bdelloid rotifers. The invention provides a method for ecologically improving the biological treatment efficiency of wastewater with low cost and easy implementation.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a method for improving the biological treatment efficiency of wastewater by adding hirudo-shaped rotifers.
Background
Compared with physicochemical treatment methods, biological treatment methods for wastewater using microorganisms such as bacteria and fungi have low cost and are environmentally friendly, but have low treatment efficiency for organic wastewater that is difficult to degrade.
In a biological wastewater treatment system, there are microorganisms, and micro-animals such as protozoa and micro metazoan. The leech-shaped rotifers in the miniature animals are a common organism beneficial to biological treatment, and have the beneficial effects of promoting the flocculation property of sludge, improving the removal rate and nitrification rate of organic matters, reducing the amount of residual sludge and the like. Chinese patent (Zl 201610119990.X) discloses a method for promoting growth of flocculating bacteria using a rotifer broth. The Chinese invention patent (CN 101432233A) discloses a method for improving the treatment efficiency of an activated sludge method and reducing the generation amount of excess sludge by utilizing the predation effect of micro animals;
the utilization of micro-animals to improve the efficiency of biological wastewater treatment is an eco-friendly method, but how to allow specific micro-animals to exist and propagate in a biological wastewater treatment reactor is a technical problem to be solved. In view of the above, the technical problem to be solved by the present invention is to provide a method for improving the biological treatment efficiency of wastewater and the viability and reproductive capacity of the bdelloid rotifers in a wastewater biological treatment reactor by using the bdelloid rotifers.
Disclosure of Invention
The invention aims to provide a method for improving the biological treatment efficiency of wastewater by adding hirudo-shaped rotifers, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a method for improving the biological treatment efficiency of wastewater by adding bdelloid rotifers comprises the following steps:
A. separating and purifying the bdelloid rotifer;
B. culturing the bdelloid rotifers;
C. adding the bdelloid rotifers;
D. breeding and breeding the leech-shaped rotifer.
Preferably, the specific method in step a is as follows: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking a hirulog from the sample in the culture dish by using a pipette under the condition that the magnification of the microscope is 10-20 times, transferring the sample into the culture dish containing 0.05-0.2% of sodium chloride solution, and shaking the culture dish for 15-30 times; placing the culture dish under an entity microscope, sucking the hirudiniform rotifers out of the culture dish solution by using a pipette gun, transferring the hirudiniform rotifers into other culture dishes containing 0.05 to 0.2 percent of sodium chloride solution, and shaking the culture dish for 15 to 30 times; the above process was repeated 4 times to obtain a hirudo-shaped rotifer free of other micro-animals.
Preferably, the specific method in step B is as follows: sieving corn flour, and taking corn granule with particle diameter less than 2 μm as food of Bdellolida, wherein the density of corn flour granule is 1 × 104～1×108And (3) cell/mL, culturing the bdelloid rotifer in a culture solution with the pH value of 6-8, aerating the culture solution to keep the dissolved oxygen at 1-5 mg/L, and performing aseptic culture at the temperature of 20-30 ℃.
Preferably, the specific method in step C is as follows: firstly adding cultured bdelloid rotifers into a wastewater biological treatment reactor, wherein the adding density of the bdelloid rotifers is 100-5000 ind/m3，After one week of feeding, if the density of the bdelloid rotifers is reduced, the bdelloid rotifers are fed again to the density of the first-fed bdelloid rotifers, so that the number of the bdelloid rotifers in the reactor is kept.
Preferably, the specific method in step D is as follows: adding 10-100 mu g/L plant source saponin when feeding the bdelloid rotifers into the wastewater bioreactor, and adding the plant source saponin again at intervals of 3-7, wherein the plant source saponin is continuously added for 4 times at the time intervals.
Preferably, the bdelloid rotifers are one or more of the rotifers, host rotifers and the like commonly found in biological processes.
Compared with the prior art, the invention has the beneficial effects that: the invention has the following remarkable advantages: 1) the bdelloid rotifers come from a wastewater biological treatment reactor and have the genetic characteristic of adapting to the wastewater biological treatment environment; 2) the corn flour as the food of the bdelloid rotifer has the characteristics of wide material source and low price; 3) the culture solution of the bdelloid rotifer not only contains a bdelloid rotifer metabolite with the function of promoting bacterial activity, but also contains organic matters such as polysaccharide and the like, so that the co-metabolism effect can be realized, and the biological treatment efficiency can be improved; 4) directly adding culture solution containing the bdelloid rotifers into the biological wastewater treatment device, and separating the rotifers from the rotifers culture solution is not needed, so that the operation steps are simplified; 5) when the bdelloid rotifers are added into the biological treatment reactor, the saponin with biological activity is added, so that the survival and reproductive capacity of the bdelloid rotifers are improved, and the bdelloid rotifers are helped to play a role in improving the biological treatment efficiency of wastewater.
Drawings
FIG. 1 is a graph showing the change of COD removal rate of printing and dyeing wastewater when adding a hirudo-shaped rotifer according to an embodiment of the present invention;
FIG. 2 is a graph showing the effect of COD removal rate of coking wastewater when adding Bdeltoid rotifers according to the second embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
in this example, referring to fig. 1, the method for improving the biological treatment efficiency of the printing and dyeing wastewater by using the bdelloid rotifers comprises the following steps:
the method for separating the bdelloid rotifers comprises the following steps: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking a hirulog from the sample in the culture dish by using a pipette under the condition that the magnification of the microscope is 15 times, transferring the sample into the culture dish containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; placing the culture dish under a solid microscope, sucking the hirudiniform rotifers out of the culture dish solution by using a pipette gun, transferring the hirudiniform rotifers into other culture dishes containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; repeating the above process 4 times to obtain a hirudo-shaped rotifer free of other micro-animals;
the cultivation method of the bdelloid rotifer comprises the following steps: sieving corn flour, and taking corn granule with particle diameter of 1 μm as food of Bdellolida, wherein the density of the corn flour granule is 1 × 106cell/mL, placing the bdelloid rotifer in a culture solution with the pH value of 7 for culturing, and placing under the condition of 25 ℃ for aseptic culture;
the method for adding the bdelloid rotifers comprises the following steps: firstly adding cultured bdelloid rotifer into a wastewater biological treatment reactor, wherein the adding density of the bdelloid rotifer is 2000ind/m3，After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first-fed bdelloid rotifers, so as to keep the number of the bdelloid rotifers in the reactor;
D. the breeding method of the bdelloid rotifer comprises the following steps: adding 50 microgram/L plant-derived saponin when feeding the bdelloid rotifer into the wastewater bioreactor, and adding the plant-derived saponin again at an interval of 4, and continuously adding for 4 times at the interval.
Analysis of biological treatment efficiency of the coating wastewater promoted by the bdelloid rotifers:
as is clear from FIG. 1, 3g/L of activated sludge (group A) was added to the dyeing wastewater, 3g/L of activated sludge and 500ind/m were added33g/L of activated sludge and 500ind/m were added to the leech-shaped rotifers (group B)3The cultured bdelloid rotifers are added once again after every other week, the plant-derived saponin with the concentration of 50 mug/L is added after 1 day of the bdelloid rotifers, the plant-derived saponin is added again after 5 days, and the feeding is continuously carried out for 4 times. Compared with the control group without adding the hirudo-shaped rotifer and the saponin, the COD removal rate of the group B is 83.1%, the COD removal rate of the group C is 90.3%, and the COD removal rate of the group A is 72.7%. Shows that the bdelloid rotifer has certain promotion effect on the biological treatment efficiency of the printing and dyeing wastewater and the saponin has the effect of obviously improving the biological activityThe physical efficiency is higher.
Example two:
this embodiment is substantially the same as the first embodiment, and is characterized in that:
in this example, referring to fig. 2, the method for improving the biological treatment efficiency of coking wastewater by using the bdelloid rotifers comprises the following steps:
the method for separating the bdelloid rotifers comprises the following steps: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking a hirulog from the sample in the culture dish by using a pipette under the condition that the magnification of the microscope is 15 times, transferring the sample into the culture dish containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; placing the culture dish under a solid microscope, sucking the hirudiniform rotifers out of the culture dish solution by using a pipette gun, transferring the hirudiniform rotifers into other culture dishes containing 0.1% sodium chloride solution, and shaking the culture dish for 20 times; repeating the above process 4 times to obtain a hirudo-shaped rotifer free of other micro-animals;
the cultivation method of the bdelloid rotifer comprises the following steps: sieving corn flour, and taking corn granule with particle diameter of 1 μm as food of Bdellolida, wherein the density of the corn flour granule is 1 × 106cell/mL, placing the bdelloid rotifer in a culture solution with the pH value of 7 for culturing, and placing under the condition of 25 ℃ for aseptic culture;
the method for adding the bdelloid rotifers comprises the following steps: firstly adding cultured bdelloid rotifer into a wastewater biological treatment reactor, wherein the adding density of the bdelloid rotifer is 2000ind/m3，After one week of feeding, if the density of the bdelloid rotifers is reduced, feeding the bdelloid rotifers again to the density of the first-fed bdelloid rotifers, so as to keep the number of the bdelloid rotifers in the reactor;
the method for improving the breeding and propagation of the bdelloid rotifers comprises the following steps: adding 50 microgram/L plant-derived saponin when feeding the bdelloid rotifer into the wastewater bioreactor, and adding the plant-derived saponin again at an interval of 4, and continuously adding for 4 times at the interval.
Primarily diluting and adjusting the COD of the coking wastewater, adding 3g/L of activated sludge into the group A, adding 3g/L of activated sludge and 500ind/m into the group B3The C group is added with 3g/L of activated sludge and 5 g/L of activated sludge00ind/m3The bdelloid rotifer and 50 microgram/L plant saponin are added with the cultured bdelloid rotifer for one time after every other week, the plant saponin with the concentration of 50 microgram/L is added after 1 day of the bdelloid rotifer, the plant saponin is added again after 5 days, and the feeding is continuously carried out for 4 times.
Collecting supernatant and measuring COD thereof to obtain three groups of comparison of COD of the coking wastewater after treatment, as shown in figure I. The rotifer culture solution promotes the biological treatment efficiency analysis of the coating wastewater:
as can be seen from fig. 2, the COD removal rate of group B was 77.3%, the COD removal rate of group C was 83.6%, and the COD removal rate of group a was 62.1%, compared to the control group to which no hirudins and saponin were added. The hirudo-shaped rotifer has a certain promotion effect on the biological treatment efficiency of the coking wastewater, and under the same microbial and micro-animal treatment conditions, the saponin has the effect of remarkably improving the biological activity, and the COD removal efficiency is higher.
In conclusion, the invention has the following remarkable advantages: 1) the bdelloid rotifers come from a wastewater biological treatment reactor and have the genetic characteristic of adapting to the wastewater biological treatment environment; 2) the corn flour as the food of the bdelloid rotifer has the characteristics of wide material source and low price; 3) the culture solution of the bdelloid rotifer not only contains a bdelloid rotifer metabolite with the function of promoting bacterial activity, but also contains organic matters such as polysaccharide and the like, so that the co-metabolism effect can be realized, and the biological treatment efficiency can be improved; 4) directly adding culture solution containing the bdelloid rotifers into the biological wastewater treatment device, and separating the rotifers from the rotifers culture solution is not needed, so that the operation steps are simplified; 5) when the bdelloid rotifers are added into the biological treatment reactor, the saponin with biological activity is added, so that the survival and reproductive capacity of the bdelloid rotifers are improved, and the bdelloid rotifers are helped to play a role in improving the biological treatment efficiency of wastewater.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A method for improving the biological treatment efficiency of wastewater by adding hirudo rotifer is characterized in that: the method comprises the following steps:
A. separating and purifying the bdelloid rotifer;
B. culturing the bdelloid rotifers;
C. adding the bdelloid rotifers;
D. breeding and breeding the leech-shaped rotifer.
2. The method for improving the efficiency of biological treatment of wastewater by adding the bdelloid rotifers according to claim 1, which is characterized in that: the specific method in the step A is as follows: collecting an activated sludge sample from a wastewater biological treatment reactor of an activated sludge method, transferring the activated sludge sample into a culture dish, placing the culture dish under a solid microscope, sucking a hirulog from the sample in the culture dish by using a pipette under the condition that the magnification of the microscope is 10-20 times, transferring the sample into the culture dish containing 0.05-0.2% of sodium chloride solution, and shaking the culture dish for 15-30 times; placing the culture dish under an entity microscope, sucking the hirudiniform rotifers out of the culture dish solution by using a pipette gun, transferring the hirudiniform rotifers into other culture dishes containing 0.05 to 0.2 percent of sodium chloride solution, and shaking the culture dish for 15 to 30 times; the above process was repeated 4 times to obtain a hirudo-shaped rotifer free of other micro-animals.
3. The method for improving the efficiency of biological treatment of wastewater by adding the bdelloid rotifers according to claim 1, which is characterized in that: the specific method in the step B is as follows: sieving corn flour, and taking corn granule with particle diameter less than 2 μm as food of Bdellolida, wherein the density of corn flour granule is 1 × 104～1×108cell/mL, culturing the bdelloid rotifer in a culture solution with the pH value of 6-8, and aerating the culture solution to dissolve oxygenKeeping the concentration at 1-5 mg/L, and placing the mixture in a sterile culture at 20-30 ℃.
4. The method for improving the efficiency of biological treatment of wastewater by adding the bdelloid rotifers according to claim 1, which is characterized in that: the specific method in the step C is as follows: firstly adding cultured bdelloid rotifers into a wastewater biological treatment reactor, wherein the adding density of the bdelloid rotifers is 100-5000 ind/m3，After one week of feeding, if the density of the bdelloid rotifers is reduced, the bdelloid rotifers are fed again to the density of the first-fed bdelloid rotifers, so that the number of the bdelloid rotifers in the reactor is kept.
5. The method for improving the efficiency of biological treatment of wastewater by adding the bdelloid rotifers according to claim 1, which is characterized in that: the specific method in the step D is as follows: adding 10-100 mu g/L plant source saponin when feeding the bdelloid rotifers into the wastewater bioreactor, and adding the plant source saponin again at intervals of 3-7, wherein the plant source saponin is continuously added for 4 times at the time intervals.
6. The method for improving the efficiency of biological treatment of wastewater by adding the bdelloid rotifers according to claim 1, which is characterized in that: the bdelloid rotifer is one or more of rotifer, Habrotrocha sp, etc. commonly used in biological treatment.
Priority Applications (1)
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| CN101433190A (en) * | 2008-12-04 | 2009-05-20 | 上海大学 | Method for promoting growth of Habrotrocha sp. using bee honey residue |
| CN101455192A (en) * | 2009-01-04 | 2009-06-17 | 上海大学 | Bdelloid rotifers mass culture method |
| CN102372404A (en) * | 2011-09-28 | 2012-03-14 | 浙江省环境保护科学设计研究院 | Bio-augmentation treatment sludge in-situ reduction method |
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| CN101433190A (en) * | 2008-12-04 | 2009-05-20 | 上海大学 | Method for promoting growth of Habrotrocha sp. using bee honey residue |
| CN101455192A (en) * | 2009-01-04 | 2009-06-17 | 上海大学 | Bdelloid rotifers mass culture method |
| CN102372404A (en) * | 2011-09-28 | 2012-03-14 | 浙江省环境保护科学设计研究院 | Bio-augmentation treatment sludge in-situ reduction method |
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| 丁国际 等: "旋轮虫在污水生物处理中的作用机制初探", 《环境科学学报》 * |
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