CN1259256C - Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water - Google Patents
Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water Download PDFInfo
- Publication number
- CN1259256C CN1259256C CN 03158644 CN03158644A CN1259256C CN 1259256 C CN1259256 C CN 1259256C CN 03158644 CN03158644 CN 03158644 CN 03158644 A CN03158644 A CN 03158644A CN 1259256 C CN1259256 C CN 1259256C
- Authority
- CN
- China
- Prior art keywords
- water
- microbial inoculum
- water purification
- culture
- reactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The present invention relates to a water cleaning bacterial agent for seedling culture and cultivation water sources, referred micro organisms of the water cleaning bacterial agent are composed of six kinds of strains, such as candida tropicalis CGMCC1012, bacillus sp. H2 CGMCC1004, heterotrophic ammonia oxidizer H9 CGMCC1010, etc. After the strains are cultured, the strains are proportioned according to the same volume proportion of the culture to form the water cleaning bacterial agent for seedling culture and cultivation circulation sea water; under the usual seedling culture and cultivation temperature, the present invention can generate lipase, amylase and proteinase, can degrade bait residue and excreta of cultured animals in the seedling culture and the cultivation, and can remove ammonia nitrogen of ultralow concentration, COD and BOD. The present invention is adapted with a corresponding bioreactor and is suitable for ammonia nitrogen removal and COD removal treatment for the circulating water in industrial high-density seedling culture and cultivation.
Description
One, technical field
The invention belongs to the microbiobacterial agent technical field, specifically, it relates to a kind of growing seedlings and the water purification microbial inoculum of breeding water, this water purification microbial inoculum relates to candida tropicalis (Candida tropicalisCGMCC1012), rhodotorula mucilaginosa (Rhodotorula mucilaginosa CGMCC1013), genus bacillus H2 (Bacillus sp.H2 CGMCC1004), genus bacillus H4 (Bacillus sp.H4CGMCC1006), heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophic ammonia oxidizer H9CGMCC1010), heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophic ammonia oxidizer H10CGMCC1011) is totally 6 kinds of bacterial strains, these bacterial strains are after cultivating, consist of the water purification microbial inoculum of growing seedlings and culturing circulating seawer according to its culture equal volume ratio proportioning, has generation lipase under the temperature growing seedlings usually and culture, amylase and proteolytic enzyme, degraded grow seedlings and culture in the bait residue and the excremental ability of cultivated animals, the effect of removing ultralow density ammonia nitrogen and COD and BOD.Adaptive with corresponding bio-reactor, be applicable to that the batch production high-density is grown seedlings and the removal of culturing the ammonia nitrogen of circulation fresh water or seawater and COD is handled.
Two, technical background
Sea-food large-scale industrialized grow seedlings and culture China obtained swift and violent development in recent years, grow seedlings and breed variety constantly increases, its scale constantly enlarges, and has greatly enriched market and has satisfied the demand of people to sea-food.But aspect a lot of, some negative impacts have also been caused.As the outburst of the disease of the pollution of immediate offshore area, cultivated animals and popular etc.Aspect seawater seed rearing and breed, its pollution is mainly derived from the movement of bait residue and cultivated animals, the general method of changing water (flowing water) that adopts of at present domestic a lot of plant and nursery, promptly extract fresh seawater through simple filtering and sterilization (also will pass through temperature raising winter), as aquaculture water, through culturing just directly discharging of certain hour (a day or shorter), some water port local or even a last plant is exactly the pump mouth of next plant.Openly grow seedlings and culture the huge waste (as the seawater temperature raising in winter) that not only causes the seawater resources and the energy, and pollution immediate offshore area, threaten the scarcity of the marine eco-environment and breeding seawater resource, even cause the generation of large-scale disease of cultivated animals and popular.Extensive sea farming, increased the output of solubilised state and suspended state material, can cause the pollution of coastal seawater to a certain extent, contaminated seawater has also restricted further developing of sea farming simultaneously, therefore exploitation and adopt batch production circulating seawer high-density to grow seedlings and cultural technique just is being subjected to extensively paying attention to and using, for solve local sea pollution problem and pass the winter culture and save energy significant.And the key that will realize the circulating seawer high-density breeding just is the maintenance and the processing of circulating seawer, and this intermediary key is again that the removal of COD in the breed recirculated water and ammonia nitrogen is handled.In general, sea farming is to COD and all strict restriction of ammonia nitrogen concentration, breed fish and require COD to be lower than 5mg/L usually, ammonia nitrogen concentration is lower than 0.5mg/L (non-ionic ammonia concentration is lower than 0.02mg/L), requirement to COD and ammonia nitrogen between nursery stage is harsh more, growing seedlings as scallop requires COD to be lower than 3mg/L, and ammonia nitrogen concentration is lower than 0.1mg/L.Solve the COD and the ammonia nitrogen problem of growing seedlings, have only two kinds of ways at present: 1) change water with breeding seawater; 2) removal of COD and ammonia nitrogen is handled.Its drawback of method of water and harm as are as previously mentioned changed in employing, do not do repetition at this, so it is not the outlet that solves the sea farming problem, also do not meet the STRATEGIES OF SUSTAINABLE DEVELOPMENT demand, therefore must eliminate the cultural technique of this roughly formula.The removal of COD and ammonia nitrogen is handled can provide technical guarantee for recycling of breeding seawater, for the discharging of reduce culturing pollutent and reduce pollution, reduce or avoid immediate offshore area cultivated animals disease generation and popular, reduce the waste of culturing the water source and the energy etc. all significantly, meet the STRATEGIES OF SUSTAINABLE DEVELOPMENT target simultaneously especially.COD and ammonia nitrogen removal processing for breeding seawater at present also do not have additive method at present, has biological method only, i.e. bioreactor process.Bio-reactor is proved by many achievements in research for nitrogen, phosphorus and the organic high efficiency of eliminating aquaculture water.Ackefors and Enell report utilizes the combination of bio-reactor and strainer can effectively remove the suspended substance of breeding seawater and nitrogen, phosphorus, its suspended substance has reduced 67～97%, COD has reduced 13～84%, and TN and TP have reduced 4～89% and 21～86% respectively.Several factors can influence the efficient of bio-reactor, and as operational conditions, technology and some other chemical factors, but a crucial factor depends on the microbial numbers that is present in wherein and the height of efficient thereof.At batch production circulating seawer high-density breeding (30kg/m
2) or grow seedlings (20 spat/mL or more than) in, particularly culture and energy efficient in order to keep winter, usually keep lower breed temperature (general about 18 degrees centigrade), require the higher cycle index of aquaculture water, usually at 4 ~ 6 times/day, and the cumulative volume that is used to remove the bio-reactor that COD and ammonia nitrogen handle is under 1/10 or the littler situation of aquaculture water cumulative volume, the COD of each cyclic water outlet and ammonia nitrogen should reach the requirement of culturing or growing seedlings, that is to say in lower temperature and hydraulic detention time to reach 50% or higher COD and ammonia nitrogen removal efficient in the time at 30 ~ 40min that therefore the core technology in the COD of the batch production circulating seawer high-density breeding and the seawater of growing seedlings and ammonia nitrogen removal are handled is exactly exploitation and the application with the adaptive efficient water purification microbial inoculum of bio-reactor.Handle problems and demand at the COD in the sea farming and ammonia nitrogen, also developed some microbial inoculums at present both at home and abroad, as the aquarium water purification microorganism of reports such as nitrated microbial inoculum, the fourth of the U.S. be good forever, Wu Wei etc. once the immobilized microorganism of report to NH in the aquaculture water
4 +-N and NO
2 -The transformation of-N etc.Though these microbial inoculums have the effect of certain removal ammonia nitrogen and purifying aquaculture seawater, but all exist significant disadvantages, the efficient of removing ammonia nitrogen as the nitrated microbial inoculum of the U.S. can not be satisfactory, its cost is too high in addition, also require regular adding, the sea-food of domestic pass is cultured the owner and is difficult to bear; Aquarium water purification microorganism and immobilized microorganism all are breadboard results of study, also have suitable distance from practical application.
Three, summary of the invention
In order to address the above problem, we handle in the research at secular aquaculture wastewater and separate, screen the micropopulation that under low water temperature the seawater water pollutant is had special removing ability, this is that common micro-organisms is difficult to accomplish, purpose of the present invention is exactly to utilize these to provide a kind of water purification microbial inoculum that is used for seawater seed rearing and breed to the micropopulation that water pollutant has special removing ability, the adaptive purification that can carry out industrial seedling rearing and culture circulating seawer water quality of this water purification microbial inoculum and bio-reactor.This water purification microbial inoculum can adapt under the short condition of low water temp, hydraulic detention time, carry out with the COD of lower cost, ultralow density and ammonia nitrogen processing plant circulating seawer that high-density is grown seedlings and aquaculture water.Specifically, this water purification microbial inoculum relates to following microorganism: candida tropicalis (Candida tropicalis CGMCC1012), rhodotorula mucilaginosa (Rhodotorulamucilaginosa CGMCC1013), genus bacillus H2 (Bacillus sp.H2 CGMCC1004), genus bacillus H4 (Bacillus sp.H4 CGMCC1006), heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophic ammonia oxidizer H9 CGMCC1010) and heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophic ammonia oxidizer H10 CGMCC1011) be totally 6 kinds of bacterial strains.Be through cultivating earlier respectively by mentioned microorganism, then, its culture mixing is used as the water purification microbial inoculum, but do not make in the water purification microbial inoculum proportional quantity between every kind of culture differ too great disparity, generally for convenience form a kind of mixing microorganisms microbial inoculum according to its culture equal volume ratio proportioning.This water purification microbial inoculum it can make ultralow density pollute COD (＜8mg/L) and ammonia nitrogen (＜0.5mg/L) water body has obtained purifying treatment.
The microorganism culturing of circulating seawer water purification microbial inoculum of the present invention: at first be that 6 strain bacterium are inoculated in separately substratum respectively, adopt malt extract medium for yeast, adopt beef broth substratum or LB substratum for genus bacillus and heterotrophic ammonia-oxidizing bacterium, usually the substratum that adopts when above-described substratum all is microorganism culturing, cultivated about 30 ℃ 2 days the inoculation back, for example, candida tropicalis (Candida tropicalis CGMCC1012), malt extract medium is adopted in the cultivation of rhodotorula mucilaginosa (Rhodotorulamucilaginosa CGMCC1013), and natural pH cultivates.For genus bacillus H2 (Bacillus sp.H2 CGMCC1004), genus bacillus H4 (Bacillus sp.H4 CGMCC1006), heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophic ammonia oxidizer H9CGMCC1010), heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophic ammonia oxidizer H10CGMCC1011), adopt beef broth substratum or LB culture medium culturing.
The preparation of the compatibility of bacterial classification and the nitrated microbial inoculum of low temperature, use: 6 above-mentioned strain bacterial classifications according to isopyknic mixed, are promptly made circulating seawer water purification microbial inoculum with its culture separately through after cultivating respectively.The microbial inoculum of making thus also can be directly used in the COD of breeding seawater and the usefulness of ammonia nitrogen removal processing.This mixing microorganisms water purification microbial inoculum can produce lipase, amylase and proteolytic enzyme, the movement of degraded bait residue and cultivated animals, reduce COD, simultaneously remove ammonia nitrogen in the water body in the mode of nitrated mode and assimilation, be particularly suitable for having with the adaptive bio-reactor of this circulating seawer water purification microbial inoculum grow seedlings and the removal of aquaculture water ultralow density ammonia nitrogen and COD is handled, adopt the circulating seawer direct biofilm of water purification microbial inoculum or adopt enlarged culturing thing biofilm, in enlarged culturing, also the part filler can be placed on biofilm in the cultivation pool.Circulating seawer water purification microbial inoculum or enlarged culturing thing are added in the bio-reactor, as: contact oxidation reactor or sbr reactor device or UASB reactor, the immersion filler is housed in the reactor, wherein filler mainly adopts elastic filler, the Ball-type packing that macromolecular material with the low surperficial potential energy that can adsorb microbial cells is made, natural plant carrier filler etc.Circulating seawer water purification microbial inoculum of the present invention can adapt to 15 ℃-20 ℃ of low water temps, has the ability that adapts to low COD and ammonia nitrogen concentration performance greater efficiency, solve under low COD and the ammonia nitrogen concentration condition grow seedlings and the COD and the ammonia nitrogen of aquaculture water are handled, make water outlet reach the water quality requirement that sea-food is cultured and grown seedlings, realization survive the winter circulating seawer culture and grow seedlings and normal temperature under the batch production circulating seawer high-density breeding and grow seedlings, have broad application prospects.
The bacterial classification of the water purification microbial inoculum that the present invention relates on September 15th, 2003 in the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms preservation, its preserving number is respectively: candida tropicalis (Candida tropicalis) CGMCC NO1012, rhodotorula mucilaginosa (Rhodotorulamucilaginosa) CGMCC1013, genus bacillus H2 (Bacillu sp.H2) CGMCC1004, genus bacillus H4 (Bacillus sp.H4) CGMCC1006, heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophic ammonia oxidizer H9) CGMCC1010 and heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophic ammonia oxidizer H10) CGMCC1011.
The culture of strains base is formed:
Malt extract medium is adopted in cultivation for candida tropicalis (Candida tropicalis CGMCC1012), rhodotorula mucilaginosa (Rhodotorula mucilaginosa CGMCC1013), and its prescription is:
Wort 1L pH nature
Beef broth substratum or LB substratum are adopted in cultivation for genus bacillus H2 (Bacillus sp.H2 CGMCC1004), genus bacillus H4 (Bacillus sp.H4 CGMCC1006), heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophic ammoniaoxidizer H9 CGMCC1010), heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophicammonia oxidizer H10 CGMCC1011), and its prescription is as follows:
Beef broth substratum: extractum carnis 5 grams, peptone 10 grams, sodium-chlor 5 grams, pH7.2
LB substratum: peptone 10 grams, yeast extract 5 grams, sodium-chlor 10 grams, pH7.2
Above-mentioned bacterial classification is inoculated in its suitable medium respectively, 30 ℃, carries out the good air culture of shaking table 200rpm and supported 2 days.
After above-mentioned 6 strain bacterium are cultivated respectively, be mixed and made into the water purification microbial inoculum by the culture of equal volume separately.
Enlarged culturing: whether test in place depends on the circumstances needs to carry out enlarged culturing, if need enlarged culturing then to use following substratum: glucose 0.5%, ammonium chloride 0.5%, peptone 0.05%, phosphoric acid salt 0.03%, sal epsom 0.01%, seawater preparation.By the inoculation of 10% inoculum size, aeration is cultivated, and it is 20 ℃ that summer room temperature, winter are controlled the enlarged culturing temperature, and 1 week was a culture cycle.
Four description of drawings
Fig. 1 circulating seawer water purification microbial inoculum is to the removal experimental result of the ammonia nitrogen of analog culture seawater
Under differing temps, remove the result of ammonia nitrogen behind Fig. 2 water purification microbial inoculum inoculation membrane formation
Five specific embodiments
In order to understand the present invention better, further specified by following embodiment, but be not limitation of the invention.
The preparation and the water conditioning of embodiment 1 circulating seawer water purification microbial inoculum
Culture of strains:
Malt extract medium is adopted in cultivation for candida tropicalis (Candida tropicalis CGMCC1012), rhodotorula mucilaginosa (Rhodotorula mucilaginosa CGMCC1013), and its prescription is:
Wort 1L pH nature
Beef broth substratum or LB substratum are adopted in cultivation for genus bacillus H2 (Bacillus sp.H2 CGMCC1004), genus bacillus H4 (Bacillus sp.H4 CGMCC1006), heterotrophic ammonia-oxidizing bacterium H9 (Heterotrophicammonia oxidizer H9 CGMCC1010), heterotrophic ammonia-oxidizing bacterium H10 (Heterotrophicammonia oxidizer H10 CGMCC1011), and its prescription is as follows:
Beef broth substratum: extractum carnis 5 grams, peptone 10 grams, sodium-chlor 5 grams, pH7.2
LB substratum: peptone 10 grams, yeast extract 5 grams, sodium-chlor 10 grams, pH7.2
Above-mentioned bacterial classification is inoculated in its suitable medium respectively, 30 ℃, carries out the good air culture of shaking table 200rpm and supported 2 days.
The preparation of the compatibility of bacterial classification and the nitrated microbial inoculum of low temperature:
6 strain bacterial classifications are through after cultivating respectively, and its culture separately according to isopyknic mixed, is promptly made and grown seedlings and culture water source water purification microbial inoculum.The microbial inoculum of making thus also can be directly used in the COD at breed water source and the usefulness of ammonia nitrogen removal processing.This breeding seawater water purification microbial inoculum is inoculated in the analog culture seawater that contains COD 30mg/L, ammonia nitrogen 5mg/L of artificial preparation by 5% inoculum size, shaking table is cultivated and is removed the ammonia nitrogen experiment under 25 ℃ of conditions, as a result, the ammonia nitrogen of the COD of 30mg/L and 5mg/L can be removed substantially fully through 50 minutes reaction.As shown in Figure 1.
Enlarged culturing and biofilm
Enlarged culturing: whether test in place depends on the circumstances needs to carry out enlarged culturing, if need enlarged culturing then to use following substratum: glucose 0.5%, ammonium chloride 0.5%, peptone 0.05%, phosphoric acid salt 0.03%, sal epsom 0.01%, seawater preparation.By the inoculation of 10% inoculum size, aeration is cultivated, summer room temperature, control 20 ℃ of enlarged culturing temperature winter, 1 week was a culture cycle.
Biofilm: adopt industrial seedling rearing and culture the circulating seawer direct biofilm of water purification microbial inoculum or employing enlarged culturing thing biofilm (in enlarged culturing, also the part filler can be placed on biofilm in the cultivation pool).Amount by 5% is added to (contact oxidation reactor or sbr reactor device or UASB reactor) in the bio-reactor with circulating seawer water purification microbial inoculum or enlarged culturing thing, the immersion filler is housed in the reactor, and (material therefor is not limit, mainly be elastic filler, the Ball-type packing that the macromolecular material of low surperficial potential energy is made, natural plant carrier filler etc.) and guarantee that specific surface area reaches 300m
2/ m
3More than, vexed exposing to the sun 2 weeks of biofilm, make circulating seawer water purification microbial inoculum evenly, fully attached to the surface of carrier filler.Control during this time about dissolved oxygen 4mg/L.
The startup of biological treatment system and prerun
After bio-reactor filler biofilm is finished, control hydraulic detention time 200min flow water inlet operation earlier 2 days, progressively even then enlargement discharge, promptly control hydraulic detention time 40min and continue 1 ~ 2 week of prerun to reaching design discharge in 2 weeks of prerun, control about dissolved oxygen 4mg/L during this time and keep about COD 20mg/L, about ammonia nitrogen concentration 2mg/L.
The operation of biological treatment system and management
Can carry out the operation of system after prerun is finished, carry out the COD and the ammonia nitrogen removal of the sea farming or the recirculated water of growing seedlings and handle.Because dissolved oxygen is higher, biological treatment system is aeration not between the breed of sea-food or nursery stage.Should in bio-reactor, add an amount of carbon source aperiodically at run duration, preferably can replenish an amount of circulating seawer water purification microbial inoculum (3ppm of bio-reactor useful volume) in every month simultaneously, if be in and culture or grow seedlings the rest periods, should keep operation by the prerun scheme.
Embodiment 2 circulating seawer water purification microbial inoculums are to the COD and the ammonia nitrogen removal result of simulated seawater
The circulating seawer water purification microbial inoculum for preparing is inoculated in the simulated seawater that contains COD30mg/L and ammonia nitrogen 5mg/L of artificial preparation by 5% inoculum size, shaking table is cultivated and is removed COD and ammonia nitrogen experiment under 25 ℃ of conditions, and the COD of 30mg/L and the ammonia nitrogen of 5mg/L can be removed substantially fully through the reaction of 50min.The result as shown in Figure 1.
Lipase, amylase and the protease activity determination of embodiment 3 water purification microbial inoculums
The active result that the circulating seawer water purification microbial inoculum for preparing is measured above-mentioned three kinds of enzymes according to standard method is as shown in table 1, the result of table 1 shows, this microbial inoculum has the enzyme of lipase, amylase and proteolytic enzyme lives, and can decompose the bait residue in the breeding seawater and the movement of cultivated animals.
The enzyme of table 1 circulating seawer water purification microbial inoculum is lived
| The kind of enzyme | Enzyme is lived |
| Lipase | 1IU/mL* |
| Amylase | 0.3U/mL** |
| Proteolytic enzyme | 0.2U/mL*** |
*: enzyme work is defined as per minute, and to discharge the required enzyme amount of 1 μ mol lipid acid from substrate be 1 international unit (IU);
*: enzyme work is defined as under 30 degree conditions in 1 hour catalysis 1mL 2% Zulkovsky starch, and to become the required enzyme amount of dextrin be 1 unit (U);
* *: enzyme work is defined as per minute, and to discharge the required enzyme amount of 1 μ g tyrosine from casein be 1 unit (U).
Simulation mini system operation after the embodiment 4 filler biofilms is to the removal of ammonia nitrogen
Circulating seawer water purification microbial inoculum is by operating on the carrier filler after the biofilm in the embodiment, the test of removing COD and ammonia nitrogen in the simulation mini system.The circulating seawer of this simulation mini system is 3m
3, bio-reactor useful volume wherein is 0.300m
3, be 1/10 of circulating seawer, load material in the bio-reactor and be the natural plant carrier filler, it is 100m that the filling degree reaches specific surface area
2/ m
3, at the beginning of 11 months by the end of October, carried out the removal experiment of different ammonia nitrogen concentrations, the results are shown in table 2.As seen from Table 2, under the lower situation of water temperature, bio-reactor after the biofilm is removal efficient basicly stable maintain 55 ~ 60% between of 35min to the COD and the ammonia nitrogen of ultralow density at the control hydraulic detention time, illustrate to have higher ammonia nitrogen removal efficient, water outlet can reach the requirement of growing seedlings and culturing.
Under embodiment 5 condition of different temperatures after the biofilm microbial inoculum to the removal of ammonia nitrogen
Microbial inoculum is carried out biofilm by method as mentioned above, is to be that the analog culture water of 4mg/L experimentizes to ammonia nitrogen concentration under the 35min condition at differing temps, hydraulic detention time then, and the result as shown in Figure 2.Show that this microbial inoculum has the ability of removing ammonia nitrogen preferably under the condition that is higher than 25 degree.
Ammonia nitrogen removal experiment in the table 2 simulation mini system
| Date (day/moon) | Water temperature (℃) | Hydraulic detention time (min) | Bio-reactor influent ammonium concentration (μ g/L) | Bio-reactor water outlet ammonia nitrogen concentration (μ g/L) | Ammonia nitrogen removal frank (%) | Bio-reactor water inlet COD (mg/L) | Bio-reactor water outlet COD (mg/L) | COD clearance (%) |
| 25/10 | 15 | 35 | 450 | 203 | 55.3 | 6.1 | 2.8 | 54.1 |
| 26/10 | 15 | 35 | 400 | 180 | 55.0 | 5.8 | 2.6 | 55.2 |
| 27/10 | 15 | 35 | 380 | 170 | 55.3 | 5.8 | 2.6 | 55.2 |
| 28/10 | 15 | 35 | 360 | 165 | 54.1 | 5.7 | 2.5 | 56.1 |
| 29/10 | 15 | 35 | 320 | 140 | 56.3 | 5.7 | 2.4 | 57.9 |
| 30/10 | 14.5 | 35 | 250 | 102 | 59.2 | 5.1 | 2.4 | 52.9 |
| 31/10 | 14.5 | 35 | 180 | 77 | 57.2 | 4.8 | 2.2 | 54.2 |
| 1/11 | 14.5 | 35 | 100 | 38 | 62 | 4.0 | 1.8 | 55.0 |
Example 5 circulating seawer water purification microbial inoculums are at the grow seedlings ammonia nitrogen removal effect of circulating seawer of scallop
At the scallop nursery of Jinying Ocean Science and Tech Development Co Ltd, Qingdao, biological treatment system is seen Fig. 2.Carried out on-the-spot circulating seawer seedling growth test, all adopted circulating seawer at whole growing-seedling period (about 2 ~ 3 weeks), during about 4 days seedlings fall the pond once.Detected the Inlet and outlet water ammonia nitrogen of biological treatment system during this time, the result shows that the ammonia nitrogen concentration of water inlet is at 50 ~ 65 μ g/L under water temperature is condition about 17 ℃, and the ammonia nitrogen concentration of water outlet maintains below the 30 μ g/L, reached the requirement that scallop is grown seedlings, what guaranteed that scallop grows seedlings carries out smoothly.Table 3 is 25 days result of operation.
The result of ammonia nitrogen was removed in the operation of table 3 system in 25 days
| Time (my god) | Hydraulic detention time (branch) | Influent ammonia nitrogen (mg/L) | Water outlet ammonia nitrogen (mg/L) | Clearance (%) |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 | 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 | 0.0395 0.0397 0.0397 0.0398 0.0401 0.0401 0.0405 0.0410 0.0411 0.0411 0.0412 0.0406 0.0405 0.0403 0.0405 0.0406 0.0406 0.0407 0.0410 0.0400 0.0406 0.0407 0.0410 0.0406 0.0407 | 0.0302 0.0303 0.0304 0.0304 0.0306 0.0307 0.0310 0.0308 0.0310 0.0315 0.0310 0.0310 0.0304 0.0301 0.0300 0.0298 0.0299 0.0301 0.0302 0.0305 0.0295 0.0295 0.0298 0.0299 0.0296 | 23.5 23.7 23.4 23.6 23.7 23.4 23.5 24.9 24.6 23.4 24.8 23.6 24.9 25.3 25.9 26.4 26.4 26.0 26.3 23.4 27.3 27.5 27.3 26.4 27.3 |
Claims (4)
1. the water purification microbial inoculum at the water source of growing seedlings and culture, the microorganism that this water source water purification microbial inoculum relates to is composed as follows: candida tropicalis CGMCC1012, rhodotorula mucilaginosa CGMCC1013, genus bacillus H2CGMCC1004, genus bacillus H4CGMCC1006, heterotrophic ammonia-oxidizing bacterium H9CGMCC1010, heterotrophic ammonia-oxidizing bacterium H10CGMCC1011, the water purification microbial inoculum is through cultivating 2 days earlier at 30 ℃ respectively by mentioned microorganism, then, its culture according to isopyknic mixed, is formed and a kind ofly grown seedlings and culture water source water purification microbial inoculum.
2. water source according to claim 1 water purification microbial inoculum, wherein the microorganism that relates to of water purification microbial inoculum adopts malt extract medium to cultivate earlier for yeast, adopt beef broth substratum or LB substratum to cultivate earlier for genus bacillus and heterotrophic ammonia-oxidizing bacterium, the cultivation back is mixed according to its culture equal volume ratio proportioning and is formed a kind of microorganism seawater water purification microbial inoculum.
3, water source according to claim 1 and 2 water purification microbial inoculum, this water purification microbial inoculum can be directly used in fresh water or sea farming water source or circulation water purification, or with the adaptive purification of carrying out industrial seedling rearing and culturing circulating seawer water quality of bio-reactor, can adapt to 15 ℃～20 ℃ of low water temps, pollute COD and carry out less than the ammonia nitrogen processing plant circulation fresh water of 0.5mg/L or seawater that high-density is grown seedlings and the processing of aquaculture water less than the ultralow density of 8mg/L.
4. water source according to claim 3 water purification microbial inoculum, the bio-reactor that wherein relates to has: contact oxidation reactor, sbr reactor device and UASB reactor, the immersion filler is housed in the reactor, filler mainly is elastic filler, the Ball-type packing that the macromolecular material of low surperficial potential energy is made, the natural plant carrier filler helps the absorption of microbial cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03158644 CN1259256C (en) | 2003-09-19 | 2003-09-19 | Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03158644 CN1259256C (en) | 2003-09-19 | 2003-09-19 | Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1597563A CN1597563A (en) | 2005-03-23 |
| CN1259256C true CN1259256C (en) | 2006-06-14 |
Family
ID=34660504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03158644 Expired - Fee Related CN1259256C (en) | 2003-09-19 | 2003-09-19 | Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1259256C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103058385B (en) * | 2011-10-20 | 2014-05-21 | 中国水产科学研究院渔业机械仪器研究所 | Process for treatment of high density aquaculture sewage |
| CN103098763B (en) * | 2013-02-06 | 2014-12-10 | 大连海洋大学 | Method of manually planting functional bacterium flora in clam worm body |
| CN104651244B (en) * | 2015-03-23 | 2017-12-15 | 河南工业大学 | A kind of yeast strain for producing acid alpha amylase and its enzymatic production method |
-
2003
- 2003-09-19 CN CN 03158644 patent/CN1259256C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1597563A (en) | 2005-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103820427B (en) | The preparation method of microorganism purification of water quality microbial inoculum | |
| CN102557323B (en) | Biological purification method for freshwater aquaculture water environment | |
| CN101475265B (en) | Water purification method for circulating water industrialized aquiculture system and complex bacterial agent thereof | |
| CN101817592B (en) | Comprehensive organism repairing method of eutrophication seawater cage culture zone | |
| CN1245151A (en) | Microbe treatment system for improving water quality of aquaculture pond | |
| CN101691547A (en) | Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation | |
| CN101503264A (en) | Method for ectopically and ecologically repairing eutrophication water | |
| CN101386823B (en) | Special effect anaerobic denitrifying bacterium and waste water processing method using thereof | |
| CN1266269C (en) | Acinetobacter for denitrification and decomposing ammonia in wastewater | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| CN107673558B (en) | Black and odorous water body purification method | |
| CN102701463B (en) | Water purifying microbial ecosystem and application thereof | |
| CN101130786A (en) | Method for culturing marine photosynthetic bacteria used for light-dark fermentation and coupling hydrogen production | |
| CN1215991C (en) | Low temperature nitrobacter agent and use thereof | |
| CN101029298A (en) | Production of efficient microbe bacteria combing agent | |
| CN107337283B (en) | Method for rapidly culturing pig breeding sewage activated sludge | |
| CN1259256C (en) | Water cleaning bacterial agent for industrial seedling growing and breeding circuluted sea water | |
| CN104585090A (en) | Transformation method for bottom material of sea cucumber breeding cofferdam | |
| CN101054242B (en) | Application of planococcus psychrotoleratus in treating sewage at low temperature | |
| CN106434424B (en) | Vibrios and application thereof with dirty seawater denitrification ability | |
| CN1340462A (en) | Bacillus subtilis method for biologically repairing culture environment of aquatic products | |
| CN111763645A (en) | Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature | |
| CN215756669U (en) | Black and odorous riverway water body prosthetic devices | |
| CN214327478U (en) | Multi-stage ecological pond for aquaculture tail water treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060614 Termination date: 20150919 |
|
| EXPY | Termination of patent right or utility model |