CN103357056B - A kind of biological spray of health type removing abnormal flavour and preparation method thereof - Google Patents
A kind of biological spray of health type removing abnormal flavour and preparation method thereof Download PDFInfo
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- CN103357056B CN103357056B CN201310310039.9A CN201310310039A CN103357056B CN 103357056 B CN103357056 B CN 103357056B CN 201310310039 A CN201310310039 A CN 201310310039A CN 103357056 B CN103357056 B CN 103357056B
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- bacillus subtilis
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- rhodopseudomonas palustris
- abnormal flavour
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Abstract
The invention provides a kind of biological spray of health type removing abnormal flavour, according to percentage by weight, raw material comprises: bacillus subtilis 10-20wt%, Lactobacillus plantarum 20-30wt%, Rhodopseudomonas palustris 50-70wt%.The present invention also provides a kind of method of above-mentioned spray, comprises the steps: step 1, provides Bacillus subtilis strain culture fluid; Step 2, provides Rhodopseudomonas palustris bacteria culture fluid; Step 3, by bacillus subtilis, Rhodopseudomonas palustris mixing, obtains described spray.The preparation method of the biological spray of health type of removing household kitchen provided by the invention and lavatory drain abnormal flavour, the effect of complex microorganism can be utilized, to the Organic substance in sewage, foul smell decomposes, and both can decompose the Organic substance in kitchen or lavatory drain, reduce abnormal flavour in a short time, improve environmental sanitation, suppress again the growth of the harmful bacteria in sewage, suppress the breeding of pathogenic antibacterial, prevent the generation of disease.
Description
Technical field
The present invention relates to environmental technology field, particularly relate to a kind of biological spray of health type removing abnormal flavour and preparation method thereof.
Background technology
Each family can have every day more or less scrub sanitary sewage, can be discharged by rinsing the most sewage of cleaning, but also have a fraction of sewage, dirt stays lower water pipe bent position, throughout the year accumulation month after month, produces abnormal flavour easily.Toilet and kitchen often can emit bad smell, and especially after the rain, this taste becomes more dense, directly has influence on the life that we are daily.A lot of niff is all from sewer in fact.If do not remove the taste that these are unpleasant, one is to pollute indoor environment, and another can cause as the insect such as Blatta seu periplaneta, damp worm.So how to remove these abnormal flavour, become many housewives' in a summer difficult problem.
Now home-use deodorant products, primary chemical series products is main, main component as maleic acid, ferrous chloride, photocatalyst etc.As patent CN102206556A discloses the agent of a kind of multipurpose high-efficiency environment-friendly deodorant, be made up of potassium carbonate, sodium tetraborate, sodium bicarbonate, sodium lauryl sulphate, it is characterized in that: the weight proportion of each component is: potassium carbonate 10-30kg, sodium tetraborate 20-60Kg, sodium bicarbonate 20-50Kg, sodium lauryl sulphate 3-8kg, be applicable to the various places that the stinks such as kitchen, room, factory, hospital, lavatory, sewage treatment plant, destructor plant pollute.For another example patent CN102526784A discloses a kind of deodorant for toilet method for making, and component is C11-C14 sodium alkyl benzene sulfonate, hydrochloric acid, ferrous chloride, zinc chloride, aluminum chloride, sodium chloride, magnesium chloride, lignin, kieselguhr, water.
But chemical deodorizing has certain limitation, often kind of chemical products is all product with strong points, when meeting other complicated chemical matrix, just can lose efficacy; After using chemical products, in water body, always have chemical residue, it may bring side effect or new pollution; Use chemical products can mask malodors, but can not change the generation of stink or stop it to distribute.
Summary of the invention
For some limitation of chemical deodorizing, if often kind of chemical products is all product with strong points, when meeting other complicated chemical matrix, just can lose efficacy; After using chemical products, in water body, always have chemical residue, it may bring side effect or new pollution; Use chemical products can mask malodors, but can not change the generation of stink or stop the problems such as it distributes, the invention provides a kind of biological spray of health type removing abnormal flavour and preparation method thereof, it comprises following technical scheme:
The present invention first aspect is to provide a kind of biological spray of health type removing abnormal flavour, and according to percentage by weight, raw material comprises:
Bacillus subtilis (Bacillussubtilis) 10-20wt%;
Lactobacillus plantarum (Lactobacillusplantarum) 20-30wt%;
Rhodopseudomonas palustris (Rhodopseudomonaspalustris) 50-70wt%.
The biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, the microorganism of removing abnormal flavour can also be can be used for, as bacillus acidophilus, saccharomyces cerevisiae etc. containing other, but be preferably made up of bacillus subtilis, Lactobacillus plantarum and Rhodopseudomonas palustris, and do not comprise other strains.
Wherein, with the gross weight of described spray for benchmark, the percentage by weight of bacillus subtilis, Lactobacillus plantarum and Rhodopseudomonas palustris is respectively 10 ~ 20wt%, 20-30wt%, 50 ~ 70wt%, is preferably 15-26wt%, 22-32wt%, 40-65wt%.
The biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, spray can also contain water, with the gross weight of spray for benchmark, the percentage by weight 0 ~ 20wt% of water.
The biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, preferably, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 × 10
10cfu/ml, Lactobacillus plantarum 1-5 × 10
8cfu/ml, Rhodopseudomonas palustris 1-2 × 10
9cfu/ml.
The present invention second aspect is to provide a kind of method preparing the biological spray of health type of above-mentioned removing abnormal flavour, and described method comprises the steps:
Step 1, provides Bacillus subtilis strain culture fluid;
Step 2, provides Rhodopseudomonas palustris bacteria culture fluid;
Step 3, by bacillus subtilis, Rhodopseudomonas palustris mixing, obtains described spray.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 1, the raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1-3wt%, culture medium 97-99wt%, at a certain temperature, carries out aerobic fermentation.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 1, the fermentation time of bacillus subtilis is preferably 1-5 days, is more preferably 1-4 days, most preferably is 1-3 days.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 1, in the sweat of described bacillus subtilis, temperature preferably controls arbitrary temp within the scope of 20-40 ° of C or temperature range.
Incubation culture medium matrix component used is preferably at least two kinds of mixture of bean cake powder, starch, yeast powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is preferably: bean cake powder 1.5-30wt%, Semen Maydis powder 2-40wt%, starch 0-15wt%, yeast powder 0-10wt%, peptone 0-5wt%, molasses 0-15wt%, sodium hydrogen phosphate 0-2wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-3wt%, water 10-99wt%.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 2, the raw material of anaerobism illumination fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 5-10wt%, culture medium 90-95wt%, at a certain temperature, anaerobism illumination fermentation is carried out.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 2, the fermentation time of Rhodopseudomonas palustris is 5-10 days, is more preferably 6-9 days, most preferably is 6-8 days.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 2, in the sweat of described Rhodopseudomonas palustris, temperature preferably controls arbitrary temp within the scope of 20-40 ° of C or temperature range.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 2, incubation culture medium matrix component used is preferably at least two kinds of mixture of yeast powder, peptone, sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its weight percent proportioning is: yeast powder 0-1wt%, peptone 0-1wt%, sodium acetate 0-3wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-1wt%, water 90-99wt%.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 3, lactobacillus bacterium liquid adopts conventional microbiological means of cultivating to carry out liquid culture, culture medium is preferably MRS, and temperature preferably controls arbitrary temp within the scope of 20-40 ° of C or temperature range, and incubation time is preferably Anaerobic culturel 1-5 days, be more preferably 2-4 days, most preferably be 3 days.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 3, by bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris and water, 10 ~ 20wt%, 20-30wt%, 50-70wt%, 0-20wt% mixing by weight percentage.
The preparation method of the biological spray of health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in step 3, preferably, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 × 10
10cfu/ml, Lactobacillus plantarum 1-5 × 10
8cfu/ml, Rhodopseudomonas palustris 1-2 × 10
9cfu/ml, preferably, the bacterium amount of each bacterium carries out regulating and controlling by centrifugal concentrating or interpolation water.
Above-mentioned technical scheme tool has the following advantages or beneficial effect:
The preparation method of the biological spray of health type of removing abnormal flavour provided by the invention, can utilize the effect of complex microorganism, to the Organic substance in sewage, foul smell decomposes, and reaches the effect of abnormal flavour and harmless treatment.Both can decompose the Organic substance in kitchen or lavatory drain, and reduce abnormal flavour in a short time, improve environmental sanitation, suppress again the growth of the harmful bacteria in sewage, and suppress the breeding of pathogenic antibacterial, prevent the generation of disease.
Detailed description of the invention
Below describe several preferred embodiment of the present invention in detail:
In following examples, strain used is:
Described bacillus subtilis is the preservation of Chinese agriculture Microbiological Culture Collection administrative center, strains A CCC10619; Described Rhodopseudomonas palustris is the preservation of Chinese agriculture Microbiological Culture Collection administrative center, bacterial strain ACCC10649; Described Lactobacillus plantarum, the preservation of Chinese agriculture Microbiological Culture Collection administrative center, bacterial strain ACCC11118.
But those skilled in the art are it is understood that according to the content of description of the present invention, also can adopt other available bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris to be prepared.Therefore, above-mentioned specific bacterial strain can not be interpreted as limitation of the scope of the invention.
Embodiment 1
Raw material forms:
Bacillus subtilis 10wt%;
Lactobacillus plantarum 25wt%;
Rhodopseudomonas palustris 60wt%;
Water 5wt%
Preparation method:
Step 1:
The raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1wt%, culture medium 99wt%, carries out 30 DEG C of aerobic fermentations 2 days.Wherein, described culture medium is, by weight percentage, the mixture of bean cake powder 3wt%, Semen Maydis powder 2wt%, starch 0wt%, yeast powder 0.5wt%, peptone 0.5wt%, molasses 0.5wt%, sodium hydrogen phosphate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 92.9wt%.
Step 2,
The raw material of anaerobic fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 10wt%, culture medium 90wt%, carries out 30 DEG C of anaerobism illumination and ferments 7 days.Described culture medium is, is by weight percentage, the mixture of yeast powder 1wt%, peptone 0wt%, sodium acetate 3wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 95.9wt%.
Step 3,
Adopted by Lactobacillus plantarum bacterium liquid conventional microbiological means of cultivating to carry out liquid culture, culture medium is MRS, and incubation time is 30 DEG C of Anaerobic culturel 3 days.Again bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, water are undertaken compositely obtaining spray with the gross weight of spray for benchmark by following bacteria containing amount and weight ratio, bacillus subtilis 10wt%, Lactobacillus plantarum 25wt%, Rhodopseudomonas palustris 60wt%, water 5wt%, wherein, bacillus subtilis bacterium amount reaches 1 × 10
10cfu/ml, Lactobacillus plantarum bacterium amount reach 1 × 10
8cfu/ml, Rhodopseudomonas palustris bacterium amount reach 1 × 10
9cfu/ml.
Embodiment 2
Raw material forms:
Bacillus subtilis 20wt%;
Lactobacillus plantarum 25wt%;
Rhodopseudomonas palustris 50wt%;
Water 5wt%
Preparation method:
Step 1:
The raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 3wt%, culture medium 97wt%, carries out 30 DEG C of aerobic fermentations 2 days.Wherein, described culture medium is, by weight percentage, the mixture of bean cake powder 3.5wt%, Semen Maydis powder 2.5wt%, starch 0wt%, yeast powder 0wt%, peptone 0.75wt%, molasses 1.0wt%, sodium hydrogen phosphate 0.3wt%, potassium dihydrogen phosphate 0.08wt%, magnesium sulfate 0wt%, water 91.87wt%.
Step 2,
The raw material of anaerobic fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 20wt%, culture medium 80wt%, carries out 30 DEG C of anaerobism illumination and ferments 7 days.Described culture medium is, is by weight percentage, the mixture of yeast powder 1.5wt%, peptone 0.5wt%, sodium acetate 4wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 93.9wt%.
Step 3,
Adopted by Lactobacillus plantarum bacterium liquid conventional microbiological means of cultivating to carry out liquid culture, culture medium is MRS, and incubation time is 30 DEG C of Anaerobic culturel 3 days.Again bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, water are undertaken compositely obtaining spray with the gross weight of spray for benchmark by following bacteria containing amount and weight ratio, bacillus subtilis 20wt%, Lactobacillus plantarum 25wt%, Rhodopseudomonas palustris 50wt%, water 5wt%, wherein, bacillus subtilis bacterium amount reaches 5 × 10
9cfu/ml, Lactobacillus plantarum bacterium amount reach 2 × 10
8cfu/ml, Rhodopseudomonas palustris bacterium amount reach 5 × 10
8cfu/ml.
Deodorizing effect is tested:
Experimental example 1: Qu Mou city water channel sewage, this water channel is black, and distributes stench, and testing its BOD is 1250mg/L, and get the smelly sewage of 1L and be sprayed into the biological spray 1ml of the present invention, after 4-8 hour, foul smell disappears, and is obviously reduced by subjective appreciation stench.
Experimental example 2: be 1mg/m at content of formaldehyde
3, area is 20m
2new decorating house in, spray the biological spray of 10ml, after 2-6 days, Detection of Air Quality content of formaldehyde≤0.10mg/m
3.
Experimental example 3: be 5mg/m at ammonia level
3, area is 15m
2pigsty in, spray the biological spray of 100ml, after 2-6 days, Detection of Air Quality ammonia level≤0.50mg/m
3.
Embodiment 4: be 0.4mg/m at benzene content
3newly purchase in automobile, spray the biological spray of 10ml, after 2-6 days, Detection of Air Quality benzene content≤0.05mg/m
3.
The preparation method of the biological spray of health type of removing abnormal flavour provided by the invention, can utilize the effect of complex microorganism, decompose the Organic substance in sewage, foul smell, reach the effect of abnormal flavour and harmless treatment.Both can decompose the Organic substance in kitchen or lavatory drain, and reduce abnormal flavour in a short time, improve environmental sanitation, suppress again the growth of the harmful bacteria in sewage, and suppress the breeding of pathogenic antibacterial, prevent the generation of disease.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (7)
1. remove the biological spray of health type of abnormal flavour, it is characterized in that, according to percentage by weight, raw material is:
Bacillus subtilis 10-20wt%;
Lactobacillus plantarum 20-30wt%;
Rhodopseudomonas palustris 50-70wt%;
Also comprise water, with the gross weight of spray for benchmark, the percentage by weight 0-20wt% of water.
2. prepare a method for spray as claimed in claim 1, it is characterized in that, comprise following step:
Step 1, provides Bacillus subtilis strain culture fluid;
Step 2, provides Rhodopseudomonas palustris bacteria culture fluid and Lactobacillus plantarum bacterium liquid;
Step 3, by bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris mixing, obtains described spray.
3. method according to claim 2, is characterized in that, also comprises the step of the raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1-3wt%, culture medium 97-99wt% in step 1, at a certain temperature, carries out aerobic fermentation 1-5 days.
4. method according to claim 3, it is characterized in that, culture medium is the mixture of bean cake powder, starch, yeast powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, sterilized water, and its weight percent proportioning is: bean cake powder 1.5-30wt%, Semen Maydis powder 2-40wt%, starch 0-15wt%, yeast powder 0-10wt%, peptone 0-5wt%, glucose 0-15wt%, sodium hydrogen phosphate 0-2wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-3wt%, sterilized water 10-92.9wt%.
5. method according to claim 2, it is characterized in that, in step 2, described Rhodopseudomonas palustris bacteria culture fluid is raw material through anaerobism illumination fermentation following weight percentage ratio and obtains: Rhodopseudomonas palustris strain 5-10wtwt%, culture medium 90-95wt%, at a certain temperature, anaerobism illumination fermentation 5-10 days is carried out.
6. the method according to claim 3 or 5, is characterized in that, described temperature controls arbitrary temp within the scope of 20-40 DEG C or temperature range.
7. method according to claim 2, is characterized in that, in step 3, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 × 10
10cfu/ml, Lactobacillus plantarum 1-5 × 10
8cfu/ml, Rhodopseudomonas palustris 1-2 × 10
9cfu/ml.
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| CN104248911B (en) * | 2014-05-13 | 2016-08-31 | 江苏绿华生物工程有限公司 | A kind of biological deodorant and preparation method thereof |
| CN106754520B (en) * | 2016-12-27 | 2021-03-05 | 上海创博生态工程有限公司 | Biological agent for controlling quantity of harmful mixed bacteria in pig house in winter and preparation method thereof |
| CN108165502B (en) * | 2017-12-15 | 2021-05-25 | 长沙理工大学 | Lactobacillus plantarum for improving flavor of fresh and wet rice noodles |
| CN109010166B (en) * | 2018-07-05 | 2022-01-28 | 上海普缇康生物技术有限公司 | Plant source small molecule oral cavity cleaning agent and preparation method thereof |
| CN109293732A (en) * | 2018-10-23 | 2019-02-01 | 宣城市福贝宠物食品有限公司 | A method of removal Yeast protein peculiar smell |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN111111423A (en) * | 2019-12-30 | 2020-05-08 | 井冈山市傲新华富育种有限公司 | Odor treatment spray for farm, and preparation method and application thereof |
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| CN102114259A (en) * | 2009-12-31 | 2011-07-06 | 上海泓宝绿色水产科技发展有限公司 | Preparation method and application for multi-functional deodorizing and peculiar smell eliminating complex microorganism spray preparation refreshing housekeeper |
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| CN102580132A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2010069007A (en) * | 2008-09-18 | 2010-04-02 | Japan Atomic Energy Agency | Antibacterial hydrogel composition |
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| EP0732396A1 (en) * | 1993-12-13 | 1996-09-18 | Asahi Kasei Kogyo Kabushiki Kaisha | Microorganism and deodorant containing cells thereof |
| CN102114259A (en) * | 2009-12-31 | 2011-07-06 | 上海泓宝绿色水产科技发展有限公司 | Preparation method and application for multi-functional deodorizing and peculiar smell eliminating complex microorganism spray preparation refreshing housekeeper |
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