CN107325992B - Bacillus subtilis strain and application thereof - Google Patents
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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Abstract
The invention relates to the field of applied biotechnology. Aims to provide a Bacillus subtilis strain and application thereof in preparing a deodorizing microbial inoculum. The preservation number of the strain in China general microbiological culture Collection center (CGMCC) is as follows: CGMCC No. 13714. The technical scheme is as follows: inoculating bacillus subtilis CGMCC13714 in an activation culture medium to culture to obtain an activation strain, and inoculating the activation strain in an amplification fermentation culture medium to culture in an amplification way to obtain a bacterial liquid; inoculating the bacterial liquid into the excrement, and keeping the temperature at 20-35 ℃. The invention provides a novel bacillus subtilis which can be applied to biological deodorization of excrement, has stable deodorization effect and is slightly influenced by temperature when being used singly; can effectively deodorize by an in-situ deodorization method, has simple and easy operation, does not need matched equipment and technology, and has small investment scale.
Description
Technical Field
The invention belongs to the technical field of applied biology, and particularly relates to a bacillus subtilis strain and application thereof.
Background
With the rapid development of economy and the improvement of living standard of people in China, people have higher requirements on the public toilet environment, and besides daily manual cleaning and cleaning sanitation, odor pollution also becomes an important issue for public toilet environment treatment. The common sterilization methods at present comprise physical sterilization and deodorization, chemical sterilization and deodorization and biological bacteriostasis and deodorization.
For public toilet environment, it is difficult to achieve sanitation by physical sterilization and deodorization methods, so chemical sterilization and deodorization, such as sodium dichloroisocyanurate, calcium hypochlorite, peracetic acid, glutaraldehyde, ethylene oxide, and other chemical bactericides, are mostly adopted at present. However, these chemical disinfectants are often accompanied by pungent odor, which causes secondary pollution to the air quality in the toilet, and improper spraying causes phenomena such as unsmooth breathing, skin allergy, and the like. Moreover, as the chemical components of the bactericide volatilize after contacting with the air, the bactericide needs to be sprayed on the same toilet for multiple times at irregular intervals to achieve the expected effect, which brings certain potential safety hazards to spraying personnel and toilet users.
In the deodorization method of the public toilet, the problems caused by chemical sterilization and deodorization can be solved by adopting biological bacteriostasis and deodorization, and no peculiar smell or secondary pollution is realized. The strain adopted by the biological antibacterial deodorization is rapid in propagation and long in survival time, can generate other beneficial bacterins in the growth process, is harmless to people and livestock, does not pollute the environment, has continuous and strong antibacterial deodorization effect, is a better choice for public toilet deodorization and environmental purification, and can be widely popularized and applied.
Disclosure of Invention
The invention mainly aims to provide a Bacillus subtilis strain.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: a Bacillus subtilis with a preservation number of: CGMCC No. 13714; the strain is preserved in China general microbiological culture Collection center (CGMCC); the address of the depository: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101; the preservation date is as follows: 3 month and 1 day 2017.
The bacillus subtilis CGMCC13714 provided by the invention is collected from breeding sewage discharged from a Sichuan double-flow pig farm, and has the characteristics that: the strain cells are in a long rod shape, and spores are in an oval shape; the colony surface is rough and opaque, is in a dirty white color, can form wrinkle when cultured in a liquid, and can survive in a meta-acid or meta-alkali environment. The physiological and biochemical properties of bacillus subtilis CGMCC13714 are shown in Table 1:
TABLE 1 physiological and biochemical Properties of Bacillus subtilis CGMCC13714
The invention also aims to provide the application of the bacillus subtilis in preparing the deodorant bacterial agent.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: the application of the bacillus subtilis in preparing the deodorizing bactericide comprises the following steps:
1) preparing bacillus subtilis CGMCC13714 bacterial liquid: inoculating Bacillus subtilis CGMCC13714 in an activation culture medium to culture to obtain an activation strain, and inoculating the activation strain in an amplification fermentation culture medium to perform amplification culture.
2) And inoculation deodorization: inoculating the bacillus subtilis CGMCC13714 bacterial liquid obtained by amplification culture into excrement, and keeping the temperature at 20-35 ℃.
Preferably: the expanded culture is to inoculate the bacillus subtilis CGMCC13714 in an expanded fermentation medium and perform shaking culture for 24-48 h at 30-35 ℃ and 120 rpm.
Preferably: the concentration of the bacillus subtilis CGMCC13714 bacterial liquid is 107～108CFU/ml。
Preferably: the inoculation amount of the bacillus subtilis CGMCC13714 bacterial liquid in the step 2) is 1-10% (V/W).
Preferably: the inoculation amount of the bacillus subtilis CGMCC13714 bacterial liquid in the step 2) is 5% (V/W).
Preferably: the components of the activation medium comprise: 10g of peptone, 10g of beef extract, 5g of yeast extract and 5g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
Preferably: the components of the expanded fermentation medium comprise: 5g of peptone, 5g of beef extract, 10g of glucose and 5g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
Preferably: when the bacillus subtilis CGMCC13714 is cultured in a solid state, the activation culture medium and the amplification fermentation culture medium also comprise 1.0 to 2.0 percent (W/V) of agar.
Preferably: preparing a solid deodorant microbial inoculum by using bacillus subtilis CGMCC 13714: mixing the bacillus subtilis CGMCC13714 bacterial liquid and a filling agent according to the mass ratio of 1:1, and placing the mixture in a constant-temperature incubator at 40 ℃ for drying and culturing for 20-24 h until the water content is 15-20%; the filler is sterilized bran.
The invention has the following beneficial effects:
1. the invention provides a novel bacillus subtilis: the bacillus subtilis CGMCC13714 is obtained by screening the waste water discharged by a culture plant, does not cause harm to the surrounding environment and ecological balance, and accords with biological safety regulations. And the bacillus subtilis is non-pathogenic bacteria, is harmless to people and livestock, and does not pollute the environment.
2. The bacillus subtilis CGMCC13714 can be applied to biological deodorization of excrement, and the strain is single in use, stable in deodorization effect, small in temperature influence and still good in field deodorization effect under the normal temperature condition. Compared with a mixed strain preparation, the preparation method has the obvious technical advantages of simple and efficient preparation process, convenience in use, packaging and transportation and easiness in forming commercialization.
3. The bacillus subtilis CGMCC13714 can effectively deodorize by an in-situ deodorization method, and compared with an ex-situ deodorization method (such as a soil deodorization method, a reactor deodorization method and the like), the deodorization operation is simple and easy to implement, matched equipment and technology are not required, and the investment scale is small. The principle of in-situ deodorization is as follows: the bacillus subtilis CGMCC13714 is based on the ingestion of substances such as saccharides, proteins and the like in excrement, can consume free oxygen in a septic tank, promote the growth of beneficial anaerobic bacteria, reduce the pH value and effectively inhibit the activity of harmful microorganisms and the rapid putrefaction and decomposition of organic matters. Meanwhile, active substances such as subtilin, polymyxin, gramicidin and the like generated in the growth process of the bacillus subtilis CGMCC13714 have obvious inhibition effect on pathogenic bacteria.
4. The bacillus subtilis CGMCC13714 can be directly inoculated (such as spraying bacterial liquid), has long lasting action time and good stability in a spore state, can well grow in excrement, occupies a favorable ecological position, inhibits the growth and the reproduction of odor-producing microorganisms, reduces the odor release strength of the excrement, and realizes the control of the odor in-situ source.
Detailed Description
The following specific examples are provided to further clarify the technical solutions and effects of the present invention for those skilled in the art.
Example 1: breeding of bacillus subtilis CGMCC13714
(1) Suspending 10mL of culture sewage discharged from Sichuan double-flow pig farm in 90mL of sterile distilled water, shaking, boiling for 5min, cooling, and performing gradient dilution with dilution degree of 10-2、10-4、10-6、10-8、10-100.1ml of the diluted solution of (A) was aspirated and plated on a screening medium at 35 ℃ to obtain a cell cultureAfter 24h of culture, multiple colonies were obtained. The screening medium used here is a beef extract peptone medium, which comprises the following components: 10g of peptone, 3g of beef extract and 5.0g of sodium chloride, adding 1000ml of water to obtain a mixture, and carrying out high-temperature sterilization treatment at 121 ℃ for 30min at a pH of 7.0-7.4.
(2) Selecting 5 bacterial colonies with different forms, vigorous growth, smooth and viscous surface, repeatedly performing streak culture on the 5 bacterial colonies for more than three times under the same culture conditions to obtain pure bacterial strains, respectively numbering as No.1, No. 2, No. 3, No. 4 and No. 5 bacterial strains, and preserving at 4 ℃ after slant culture. The five strains are respectively cultured by using an activation culture medium, 1ml of a bacterial liquid obtained by culture is inoculated into a 1L beaker filled with 100g of human excrement, and after the bacterial liquid acts for 3d at the temperature of 30 ℃, the odor intensity, the ammonia concentration and the hydrogen sulfide concentration in the five beakers are respectively measured. Wherein, the strength of odor in the beaker inoculated with the No. 3 strain is reduced by 26.8 percent, the concentration of ammonia gas is reduced by 39.5 percent, the concentration of hydrogen sulfide is reduced by 35.2 percent, and the odor is far higher than that of other four strains, and the primary judgment shows that the odor removal capability is strongest, which is named as FJN.
(3) Strain identification
1) And morphological characteristics: the cells are rod-shaped, gram-positive, 0.7-1 μm wide and 2-3 μm long; the capsule is not contained, and the flagella is generated in the week and can move; culturing at 37 deg.C for 2-3 days to form spore with size of 0.6-0.9 × 1-1.5 μm, which is elliptical or cylindrical and is located at the center or slightly deviated from the thallus.
2) And culture properties: the strain cells are in a long rod shape, and spores are in an oval shape; the colony surface is rough and opaque, is dirty white, and can form wrinkles during liquid culture.
3) And molecular biological identification: extracting the whole genome DNA of the strain, carrying out PCR amplification on a 16S rDNA fragment, sequencing, comparing the sequencing result in NCBI (national center for Biotechnology information) and displaying that the strain is Bacillus subtilis.
Example 2: application of bacillus subtilis CGMCC13714 in feces deodorization
1. Preparation of culture medium and inoculated bacterial liquid
Solid activation medium: 10g of peptone, 10g of beef extract, 5g of yeast extract, 5.0g of sodium chloride and 15g of agar, and adding water to 1000ml, wherein the pH value is 7.0-7.4. Liquid expanded fermentation medium: 5g of peptone, 5g of beef extract, 10g of glucose and 5.0g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
Inoculating bacillus subtilis CGMCC13714 into an activation culture medium for activation culture for 24h to obtain an activated strain for later use; inoculating the activated strain to an expanded fermentation medium, and performing shake culture for 24h at 35 ℃ under the condition of 120rmp to obtain an inoculated bacterial liquid for later use.
2. Deodorizing excrement
Inoculating 5ml of the inoculated bacterial liquid into a 1L beaker filled with 500g of human excrement (the inoculation amount is 1.0 percent, V/W), and sealing the beaker by using a preservative film; meanwhile, a control without adding bacteria liquid is carried out, three times of experiments are set, and the temperature is kept at 30 ℃. After 5d, the odor intensity, ammonia concentration and hydrogen sulfide concentration were measured, and the measurement results are shown in table 2 below.
TABLE 2 deodorizing Effect of Bacillus subtilis CGMCC13714 Strain (30 ℃ C.)
As can be seen from Table 2, when the temperature is 30 ℃, after Bacillus subtilis CGMCC13714 is added into feces to act for 5 days, the odor intensity, the ammonia gas concentration and the hydrogen sulfide concentration are all reduced compared with the illumination, wherein the odor intensity is reduced by 29.3%, the ammonia gas concentration is reduced by 41.0% and the hydrogen sulfide concentration is reduced by 37.0%.
Example 3: application of bacillus subtilis CGMCC13714 in feces deodorization
1. Preparation of culture medium and inoculated bacterial liquid
Activating a culture medium: 10g of peptone, 10g of beef extract, 5g of yeast extract, 5.0g of sodium chloride, and water until the volume is 1000ml, wherein the pH value is 7.0-7.4. Liquid expanded fermentation medium: 5g of peptone, 5g of beef extract, 10g of glucose and 5.0g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
Inoculating bacillus subtilis CGMCC13714 into an activation culture medium for activation culture for 24h to obtain an activated strain for later use; inoculating the activated strain to an expanded fermentation medium, and performing shake culture at 30 ℃ and 120rmp for 24h to obtain an inoculated bacterial liquid for later use.
2. Deodorizing excrement
Inoculating 5ml of the inoculated bacterial liquid into a 1L beaker filled with 500g of human excrement (the inoculation amount is 1.0 percent, V/W), and sealing the beaker by using a preservative film; meanwhile, a control without adding a bacterial solution is carried out, three times of experiments are set for each group, and the temperature is kept at 20 ℃. After 5d, the odor intensity, ammonia concentration and hydrogen sulfide concentration were measured, and the measurement results are shown in table 3 below.
TABLE 3 deodorizing Effect of Bacillus subtilis CGMCC13714 Strain (20 ℃ C.)
| Measurement index | Control | Strain treatment | Reduction ratio (%) |
| Strength of Odor (OU) | 176750 | 155660 | 11.8 |
| Ammonia concentration (mg/m)3) | 34.2 | 23.1 | 32.5 |
| Hydrogen sulfide concentration (mg/m)3) | 105.2 | 86.5 | 17.8 |
As can be seen from Table 3, when the temperature is 20 ℃, the feces are added with the Bacillus subtilis CGMCC13714 bacterial liquid to act for 5 days, the odor intensity is reduced by 11.8%, the ammonia concentration is reduced by 32.5%, and the hydrogen sulfide concentration is reduced by 17.8%. The deodorizing effect is reduced as compared with that at a temperature of 30 c, but a certain deodorizing effect is still exhibited.
Example 4: application of bacillus subtilis CGMCC13714 in feces deodorization
The preparation methods of the culture medium and the inoculated bacterial liquid are the same as that in the example 2, three groups of parallel experiments are set for fecal deodorization treatment, the inoculum sizes (V/W) of the bacterial liquids in the three groups are respectively 2%, 5% and 10%, the temperature is kept within the range of 25-35 ℃, the odor intensity, the ammonia concentration and the hydrogen sulfide concentration are measured after 5 days, and the measurement results are shown in the following table 4.
TABLE 4 deodorizing effect of Bacillus subtilis CGMCC13714 strain
As can be seen from Table 4, after the feces are added with the bacillus subtilis CGMCC13714 bacterial liquid with different inoculum sizes and acted for 5 days, the deodorization effect shows a trend of increasing firstly and then decreasing along with the increase of the inoculum size, wherein when the inoculum size is 5%, the deodorization effect is best, the odor intensity is decreased by 35.4%, the ammonia gas concentration is decreased by 50.0%, and the hydrogen sulfide concentration is decreased by 47.5%.
Example 5: bacillus subtilis CGMCC13714 applied to deodorization in public toilets
The preparation method of the culture medium and the inoculated bacterial liquid is the same as that of the example 2, the environmental temperature is 30-35 ℃ in a certain public toilet in Wu-Hou district of metropolis, and the squatting pan is sprayed with the bacterial liquid of bacillus subtilis CGMCC13714 (the spraying amount can be adjusted according to the flow rate and the odor degree of the public toilet, for example, the spraying amount is 12 mL/squatting pan). The odor intensity, ammonia concentration and hydrogen sulfide concentration in the toilet before and after spraying at 5d were measured, respectively, and the measurement results are shown in Table 5 below.
TABLE 5 deodorization Effect of Bacillus subtilis CGMCC13714 Strain (30-35 deg.C)
| Measurement index | Control | Strain treatment | Reduction ratio (%) |
| Strength of Odor (OU) | 3562 | 1685 | 52.7 |
| Ammonia concentration (mg/m)3) | 3.5 | 2.1 | 40.0 |
| Hydrogen sulfide concentration (mg/m)3) | 16.7 | 6.5 | 61.1 |
As can be seen from Table 5, after the Bacillus subtilis CGMCC13714 bacterial liquid 5d is sprayed in the public lavatory, the odor intensity, the ammonia concentration and the hydrogen sulfide concentration are all obviously reduced compared with those before the spraying, wherein the odor intensity is reduced by 52.7%, the ammonia concentration is reduced by 40.0%, the hydrogen sulfide concentration is reduced by 61.1%, and the deodorization effect is equivalent to that of the small-scale deodorization effect of the first embodiment.
Example 6: preparation of deodorizing bacterial agent
1. Liquid deodorant bacterial agent: preparation and activation culture of the culture medium the same as in example 3, the activated strain was inoculated into an expanded fermentation medium and cultured under shaking at 30 ℃ under 120rmp for 48 hours to obtain a liquid deodorant microbial inoculum. Expanding a fermentation medium: 5g of peptone, 5g of beef extract, 10g of glucose and 5.0g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
2. Solid deodorant bacterial agent: mixing the liquid deodorant microbial inoculum and sterilized bran according to the mass ratio of 1:1, and placing the mixture in a constant-temperature incubator at 40 ℃ for drying and culturing for 21h to reduce the water content to 15-20% to obtain the solid deodorant microbial inoculum.
Sequence listing
<110> Youdoujia environmental protection equipment engineering Co., Ltd
<120> bacillus subtilis strain and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
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<211> 1503
<212> DNA
<213> Bacillus subtilis
<400> 1
cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgagc ggacagatgg 60
gagcttgctc cctgatgtta gcggcggacg ggtgagtaac acgtgggtaa cctgcctgta 120
agactgggat aactccggga aaccggggct aataccggat ggttgtttga accgcatggt 180
tcaaacataa aaggtggctt cggctaccac ttacagatgg acccgcggcg cattagctag 240
ttggtgaggt aacggctcac caaggcaacg atgcgtagcc gacctgagag ggtgatcggc 300
cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttccg 360
caatggacga aagtctgacg gagcaacgcc gcgtgagtga tgaaggtttt cggatcgtaa 420
agctctgttg ttagggaaga acaagtaccg ttcgaatagg gcggtacctt gacggtacct 480
aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540
ttgtccggaa ttattgggcg taaagggctc gcaggcggtt tcttaagtct gatgtgaaag 600
cccccggctc aaccggggag ggtcattgga aactggggaa cttgagtgca gaagaggaga 660
gtggaattcc acgtgtagcg gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag 720
gcgactctct ggtctgtaac tgacgctgag gagcgaaagc gtggggagcg aacaggatta 780
gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttaggggg tttccgcccc 840
ttagtgctgc agctaacgca ttaagcactc cgcctgggga gtacggtcgc aagactgaaa 900
ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960
cgcgaa 1020
gaaccttacc aggtcttgac atcctctgac aatcctagag ataggacgtc cccttcgggg 1080
gcagagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt 1140
cccgcaacga gcgcaaccct tgatcttagt tgccagcatt cagttgggca ctctaaggtg 1200
actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga 1260
cctgggctac acacgtgcta caatggacag aacaaagggc agcgaaaccg cgaggttaag 1320
ccaatcccac aaatctgttc tcagttcgga tcgcagtctg caactcgact gcgtgaagct 1380
ggaatcgcta gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg gccttgtaca 1440
caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta accttttagg 1500
agccagccgc cgaaggtggg acagatgatt ggggtgaagt cgtaacaagg tagccg 1503
Claims (7)
1. An application of bacillus subtilis in preparing a fecal deodorizing microbial inoculum is characterized in that: the bacillus subtilis (Bacillus subtilis) has a preservation number in the common microorganism center of China Committee for culture Collection of microorganisms as follows: CGMCC No. 13714; the preservation date is as follows: 3 months and 1 day in 2017;
the method specifically comprises the following steps:
1) preparing bacillus subtilis CGMCC No.13714 bacterial liquid: inoculating bacillus subtilis CGMCC No.13714 in an activation culture medium to be cultured to obtain an activation strain, and inoculating the activation strain in an amplification fermentation culture medium to be subjected to amplification culture;
2) and inoculation deodorization: inoculating the bacillus subtilis CGMCC No.13714 bacterial liquid obtained by the enlarged culture in human excrement, and keeping the temperature at 20-35 ℃.
2. Use according to claim 1, characterized in that: the expanding culture in the step 1) is to inoculate the bacillus subtilis CGMCC No.13714 in an expanding fermentation medium and shake culture for 24-48 h under the conditions of 30-35 ℃ and 120 rpm.
3. Use according to claim 1, characterized in that: the inoculation amount of the bacillus subtilis CGMCC No.13714 bacterial liquid in the step 2) is 1-10% V/W.
4. Use according to claim 3, characterized in that: the inoculation amount of the bacillus subtilis CGMCC No.13714 bacterial liquid in the step 2) is 5 percent V/W.
5. Use according to claim 1, characterized in that: the components of the activation medium comprise: 10g of peptone, 10g of beef extract, 5g of yeast extract and 5g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4; the components of the expanded fermentation medium comprise: 5g of peptone, 5g of beef extract, 10g of glucose and 5g of sodium chloride, and adding water to 1000ml, wherein the pH value is 7.0-7.4.
6. Use according to claim 5, characterized in that: the activating culture medium and the expanding fermentation culture medium also comprise 1.0 to 2.0 percent of agar W/V.
7. Use according to claim 1, characterized in that: preparing a solid deodorant microbial inoculum by using bacillus subtilis CGMCC No. 13714: mixing the bacillus subtilis CGMCC No.13714 bacterial liquid and the filler according to the mass ratio of 1:1, and placing the mixture in a constant-temperature incubator at 40 ℃ for drying and culturing for 20-24 h until the water content is 15-20%; the filler is sterilized bran.
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| CN108239614B (en) * | 2017-12-20 | 2020-12-04 | 华中农业大学 | A Bacillus strain for removing the odor of sheep manure and its application |
| CN108841747B (en) * | 2018-06-25 | 2020-08-14 | 青岛农业大学 | Protease-producing Bacillus subtilis and method of use |
| CN110438024B (en) * | 2019-04-17 | 2023-03-28 | 浙江亲水园生物科技有限公司 | Bacillus subtilis strain and application thereof |
| CN110923177B (en) * | 2019-12-31 | 2021-07-27 | 天津开发区坤禾生物技术有限公司 | Composite microbial inoculum for preventing and treating pond moss as well as preparation method and application thereof |
| CN112831438B (en) * | 2021-01-25 | 2023-05-02 | 瑞燃(上海)环境工程技术有限公司 | Bacillus subtilis for deodorizing kitchen waste and application thereof |
| CN112877250A (en) * | 2021-03-02 | 2021-06-01 | 盐城师范学院 | Biological agent for efficiently deodorizing excrement and preparation method and application thereof |
| CN113736686B (en) * | 2021-07-07 | 2024-04-16 | 甘肃农业大学 | Microbial deodorant for desulfurizing and deaminizing and preparation method thereof |
| CN114410520B (en) * | 2022-01-11 | 2024-06-04 | 山东蔚蓝生物科技有限公司 | Bacillus subtilis and application thereof in fecal compost deodorization |
| CN114455784A (en) * | 2022-03-11 | 2022-05-10 | 洛阳博鑫生物工程科技有限公司 | Online direct drainage treatment method and treatment equipment for fecal sewage in vehicle-mounted toilet of high-speed rail motor train unit |
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