CN114617135A - Biological agent with moss deactivating effect and preparation method and application thereof - Google Patents
Biological agent with moss deactivating effect and preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/14—Insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Biodiversity & Conservation Biology (AREA)
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- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Farming Of Fish And Shellfish (AREA)
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Abstract
The invention discloses a biological preparation with a moss deactivating effect, a preparation method and application thereof, wherein the biological preparation is prepared by the following steps: and mixing the gallnut powder obtained by crushing with the bacillus subtilis according to a certain mass percentage to obtain the biological preparation rich in the plant tannin and the bacillus subtilis. The prepared biological agent is added with brown sugar water according to a certain feed-liquid ratio for activation treatment, can be used for effectively inhibiting and killing moss in aquaculture, improving water quality, and is non-toxic, harmless, residue-free and pollution-free to environment and other aquatic organisms, so that the problem of moss prevention and control in aquaculture is solved.
Description
Technical Field
The invention belongs to the field of aquaculture, relates to the technical field of killing of moss in crayfish and river crab culture ponds, and particularly relates to a preparation method of a biological agent and application of the biological agent in the field of killing of moss.
Background
With the improvement of the living standard of residents in China, the consumption structure is continuously optimized and improved, the specific gravity of aquatic products in the dietary structure is continuously increased, and the total output value of the aquaculture industry is in a stable increasing state. While the aquaculture industry in China gains huge achievements, the problems of pollution around the aquaculture water area, moss prevention and control and the like are also faced. The moss is a product of excessive propagation of algae in the water body, and the growth of a large amount of moss damages the algae phase balance of the water body and seriously damages the water quality, so that the excessive moss in the pond has great harm to aquaculture, and serious economic loss is caused. The currently adopted moss killing technology comprises a physical method, a chemical method, a biological method and the like. The use of physical methods is time-consuming and labor-consuming; the chemical method comprises prometryn powder, copper sulfate and the like, has great environmental pollution, remains and is unsafe; the use of biological methods such as microecologics is costly and has little effect.
Galla chinensis is a general term for insect gall parasitic on Rhus chinensis in Rhus of Anacardiaceae. China is the main producing country and the processing country of gallnuts. Historically, the main function of the development of the gallnut industry in China is medicinal. According to the records of medical history book 'compendium of materia Medica': galla chinensis is sour, astringent and cold, and has effects of stopping nocturnal emission, relieving diarrhea, astringing lung, lowering fire, astringing dampness, astringing intestine, healing sore, and stopping bleeding. Modern medicine proves that the gallnut contains tannic acid, and the gallnut has the effects of local hemostasis, antibiosis, detoxification and the like because the tannic acid can form insoluble compounds with some metals, alkaloids and the like and can denature protein. It is recorded in Kaibao Bencao that it has the actions of astringing lung to reduce pathogenic fire, astringing intestine to stop diarrhea, astringing dampness to heal wound, arresting sweating and stopping bleeding. Can be used for treating chronic cough due to lung deficiency, cough due to lung heat, traumatic hemorrhage, hematochezia, hemorrhoid, skin wet rot, chronic diarrhea, chronic dysentery, spontaneous perspiration, night sweat, skin sore, diabetes, etc. Gallnut has been reported to have an in vitro antibacterial effect on various pathogenic bacteria such as staphylococcus epidermidis, pseudomonas aeruginosa, staphylococcus aureus, candida albicans and the like, and is commonly used for treating anorectal diseases and bacterial cariogenic diseases in clinic at present. Because of the inhibition effect of gallnut on aquatic pathogenic bacteria in various aquaculture, gallnut powder is often used as a pond cleaning medicament in aquaculture.
The dead moss floats on the water surface and is slowly decomposed under the action of microorganisms, so that the inventor is inspired to separate a bacillus from the rotten moss, and the bacterial strain is found to have good growth inhibition effect on the moss through a laboratory culture test. The bacillus used in the technology is bacillus subtilis (China general microbiological culture Collection center, preservation number: CGMCC 1.3358), and in addition, the Chinese gall powder can effectively kill moss according to research findings. Thus, the inventor prepares a preparation capable of effectively killing moss by mixing the Chinese gall powder with the bacterial strain. The main components of the product are plant extract, antagonistic bacteria complex flora and moss lyase. The method can directionally identify and destroy the cell wall structure of the moss, gradually decompose the moss and convert the moss into organic matter components which can be used by the aquatic weeds, eliminate pollution damage of dead moss to water quality, cannot damage aquatic weeds such as hydrilla verticillata and elodea nutans in the aquaculture water body, cannot generate stress to aquaculture animals, does not have drug residues, and has good biological safety.
Disclosure of Invention
The purpose of the invention is as follows: in order to solve the problems in the prior art, the invention provides a preparation method and application of a preparation with moss killing effect.
The technical scheme is as follows: a biological preparation with a moss deactivating effect comprises Chinese gall powder and bacillus subtilis according to a mass ratio of 0.5-1: 10, wherein the activity of the bacillus subtilis is more than or equal to 1 multiplied by 108CFU/g。
The nutgall powder is crushed with 50-100 meshes.
The preparation method of the biological preparation with the green-removing moss function comprises the step of uniformly mixing the gallnut powder obtained by crushing and the bacillus subtilis according to a proportion to obtain the biological preparation which is rich in the plant tannin and the bacillus subtilis and has the green-removing moss function.
The biological preparation with the moss deactivating effect is applied to preventing and treating moss in water and aquaculture.
Before use, the biological preparation with the green-removing moss function is added into brown sugar water in proportion for activation treatment.
The brown sugar water comprises the following components in percentage by mass: 5-10 parts of brown sugar: 1 is configured.
The feed-liquid ratio of the biological agent to the brown sugar water is 1: 20-25.
The activation temperature is 25-30 ℃, and the activation time is 6-8 h.
Has the advantages that: the invention provides a preparation method of a preparation with the green moss removing effect, researches show that the biological preparation can be used for effectively inhibiting and killing green moss in aquaculture, improving water quality, and solving the problem of green moss prevention and control in aquaculture, and is non-toxic, harmless, residue-free and pollution-free to environment and other aquatic organisms.
Drawings
FIG. 1 shows the killing effect of a biological agent on moss (moss floats in large amount 3 days after use)
FIG. 2 shows the killing effect of a biological agent on moss (decomposition of moss 8 days after use)
Detailed Description
The present invention will be described in further detail with reference to specific embodiments. The specific examples described in the following embodiments of the present invention are merely illustrative of specific embodiments of the present invention and are intended to be illustrative of the present invention and not to be construed as limiting the present invention.
Example 1: preparation method and application of preparation with green-removing moss function
Pulverizing Galla chinensis powder with particle size of 50 mesh and Bacillus subtilis (China general microbiological culture Collection center, preservation number: CGMCC 1.3358, from Xiamen Yongke Biotech Co., Ltd.) (activity is not less than 1 × 10)8CFU/g) is fully mixed according to the mass ratio of 0.5:10 to obtain the biological preparation rich in the plant tannin and the bacillus subtilis. And (3) mixing the prepared biological agent according to a material-liquid ratio of 1: 20(kg/kg) brown sugar water (10: 1(kg/kg) water) was added at 25 deg.CThen, activation treatment is carried out for 8 h. In the outbreak period of moss, sodium humate is matched, and the moss is splashed into the whole pond after being activated according to the dosage of 100g of water surface per mu (the water depth is about 10 m). Generally, the aquatic weeds turn yellow on the 2 nd day, gradually separate from the aquatic weeds and float upwards with the time (see figure 1), and the moss dies and decomposes around a week (see figure 2).
Example 2: preparation method and application of preparation with green-removing moss function
Pulverizing Galla chinensis powder with particle size of 80 meshes and Bacillus subtilis with activity of 1 × 10 or more8CFU/g) is fully mixed according to the mass percentage of 0.8:10 to obtain the biological preparation rich in the plant tannin and the bacillus subtilis. And (3) mixing the prepared biological agent according to a material-liquid ratio of 1: 20(kg/kg) was added with brown sugar water (water: brown sugar 5: 1(kg/kg)) and activated at 28 ℃ for 7 hours. In the moss outbreak period, sodium humate is matched, and the full pond sprinkling is carried out after the activation according to the dose of 100g per mu of water surface (the water depth is about 10 meters). Generally, the aquatic weeds turn yellow on the 2 nd day, gradually separate from the aquatic weeds and float upwards along with the increase of time, and the moss dies and decomposes around a week.
Example 3: preparation method and application of preparation with moss deactivating effect
And (3) sufficiently mixing the gallnut powder with the granularity of 100 meshes obtained by crushing and the bacillus subtilis (the activity is more than or equal to 1 multiplied by 108CFU/g) according to the mass percentage of 1:10 to obtain the biological preparation rich in the plant tannin and the bacillus subtilis. And (3) mixing the prepared biological agent according to a material-liquid ratio of 1: brown sugar water (water: brown sugar 10: 1(kg/kg)) was added to 25(kg/kg) and the activation treatment was carried out at 30 ℃ for 6 hours. In the moss outbreak period, sodium humate is matched, and the full pond sprinkling is carried out after the activation according to the dose of 100g per mu of water surface (the water depth is about 10 meters). Generally, the aquatic weeds turn yellow on the 2 nd day, gradually separate from the aquatic weeds and float upwards along with the increase of time, and the moss dies and decomposes around a week.
Example 4: comparative test without activation
Gallnut powder with the granularity of 100 meshes obtained by crushing is taken and fully mixed with the bacillus subtilis (the activity is more than or equal to 1 multiplied by 108CFU/g) according to the mass percentage of 1:10 to obtain the biological agent rich in the plant tannin and the bacillus subtilis. In the moss outbreak period, sodium humate is matched, and the full pond sprinkling is carried out after the activation according to the dose of 100g per mu of water surface (the water depth is about 10 meters). The moss does not obviously float and die after being used for one week.
Example 5: comparative test with Bacillus alone
Bacillus subtilis (the activity is more than or equal to 1 multiplied by 108CFU/g) is added according to the feed-liquid ratio of 1: brown sugar water (water: brown sugar 10: 1(v/v)) was added at 25(kg/kg) and the activation treatment was carried out at 30 ℃ for 6 hours. In the moss outbreak period, sodium humate is matched, and the full pond sprinkling is carried out after the activation according to the dose of 100g per mu of water surface (the water depth is about 10 meters). The moss does not obviously float and die after being used for one week.
Example 6: laboratory moss cultivation experiment
And (3) sufficiently mixing the gallnut powder with the granularity of 50 meshes obtained by crushing and the bacillus subtilis (the activity is more than or equal to 1 multiplied by 108CFU/g) according to the mass percentage of 1:10 to obtain the biological preparation rich in the plant tannin and the bacillus subtilis. And (3) mixing the prepared biological agent according to a material-liquid ratio of 1: 20(kg/kg) was added with brown sugar water (water: brown sugar 10: 1(kg/kg)) and activated at 30 ℃ for 6 hours to obtain a medicinal solution. Placing 200mL of the above medicinal liquid in 200mL culture flask, adding 2g of moss in vigorous growth stage into 200mL river water of control group, culturing in sunlight, and observing. The result shows that the control solution is green and has light fishy smell after the moss is cultivated for 24 hours, and the medicament group has dark color; after 48 hours, the color deepens and becomes black, and the fish smell is generated. Experiments show that the preparation prepared by the invention has an inhibiting effect on moss.
The above description is only of the preferred embodiments of the present invention, and it should be noted that: it will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the principles of the invention and these are intended to be within the scope of the invention.
Claims (8)
1. The biological preparation with the green-removing moss function is characterized by comprising Chinese gall powder and bacillus subtilis according to a mass ratio of 0.5-1: 10, wherein the activity of the bacillus subtilis is more than or equal to 1 multiplied by 108CFU/g。
2. The biological preparation with the green-removing moss function according to claim 1, wherein the nutgall powder is crushed by 50-100 meshes.
3. The method for preparing a biological agent with moss deactivating effect as claimed in claim 1 or 2, wherein the gallnut powder obtained by pulverizing is mixed with the bacillus subtilis in proportion to obtain the biological agent with moss deactivating effect, which is rich in vegetable tannin and bacillus subtilis.
4. The use of the biological agent with moss deactivating effect of claim 1 in preventing and treating moss in water and aquaculture.
5. The use of claim 4, wherein the biological preparation with green-removing moss effect is added into brown sugar water in proportion for activation treatment before use.
6. Use according to claim 5, wherein the ratio of brown sugar water to water by mass: 5-10 parts of brown sugar: 1 is configured.
7. The use of claim 5, wherein the mass-to-liquid ratio of the biological agent to the brown sugar water is 1: 20-25.
8. Use according to claim 5, wherein the activation temperature is 25-30 ℃ and the activation time is 6-8 h.
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| CN202210248182.9A CN114617135B (en) | 2022-03-14 | 2022-03-14 | Biological agent with green removing function and preparation method and application thereof |
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| CN114617135B CN114617135B (en) | 2024-04-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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