CN112777752A - Composite preparation for inhibiting growth of moss in aquaculture pond and application - Google Patents
Composite preparation for inhibiting growth of moss in aquaculture pond and application Download PDFInfo
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- CN112777752A CN112777752A CN202011508842.XA CN202011508842A CN112777752A CN 112777752 A CN112777752 A CN 112777752A CN 202011508842 A CN202011508842 A CN 202011508842A CN 112777752 A CN112777752 A CN 112777752A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
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Abstract
The invention belongs to the technical field of aquaculture, and particularly relates to a composite preparation for inhibiting moss growth in an aquaculture pond and an application thereof, wherein the composite preparation comprises the following components in parts by mass: 0.1-25 parts of cellulase, 0.1-35 parts of pectinase, 0.1-75 parts of bacillus subtilis powder, 25-125 parts of lanthanum oxide, 1-55 parts of sodium lauryl sulfate, 85-250 parts of citric acid and 900 parts of anhydrous sodium sulfate. The invention integrates the characteristics of rapid direct reaching into moss cells, rapid action, obvious effect, safety to cultivated organisms and ecology and the like, can be directly put into a cultivation water body, and has the advantages of small usage amount, long storage period, high activity and convenient use.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a composite preparation for inhibiting the growth of moss in an aquaculture pond and application thereof.
Background
The phytoplankton is the primary productivity in the aquaculture water body and is the basis for maintaining the ecological balance and stability of the water body, so at the beginning stage of aquaculture, the water fertilizing work must be done to ensure the biomass of the phytoplankton in the water body. However, the temperature of water is low and the water is shallow, so that the phytoplankton is not cultured and the moss grows.
Lichen is a plant of aquatic mosses, emerald green, growing in water or in the wet and cloudy areas on land. The moss has certain harm to aquaculture. (1) The moss can compete for nutrients with waterweeds and other algae, excessively consume water body nutrients, destroy normal substance metabolism of the pond and make the culture water body thin. The consequence of water thinning is that the growth of aquatic weeds is inhibited, the periodic growth of plankton is destroyed, and the algal phase of the water body is unbalanced. All of these can affect the normal growth of aquatic animals such as crayfish, crabs, and fish. (2) The culture water body with a large amount of grown moss is clear and transparent, the sunlight directly irradiates the pond, the stress reaction of crayfish, crab and fish is increased, and the immunity of cultured animals is reduced and pathological changes are easily caused by the stress reaction for a long time. (3) The moss grows and breeds in a large quantity, is suspended in water like a net of heaven and earth, is easy to entangle crayfish, crab and fish, cannot struggle basically, and can only starve or die alive. (4) The dead moss blows to the corners of the pond or sinks to the bottom to rot with wind, and besides emitting foul smell, the dead moss also consumes dissolved oxygen in a water body and generates a lot of toxic and harmful substances in the decomposition process, so that the dissolved oxygen in the aquaculture water body is reduced, the harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide are increased, the water quality is damaged, and the crayfish, the crabs and the fish are killed due to oxygen deficiency and poisoning, and the healthy aquaculture is damaged. Therefore, the control of moss is an important technical link in the aquaculture process.
At present, the main methods for controlling moss in aquaculture are 3. The first is a light-shielding method, which inhibits photosynthesis of moss by deepening water depth and using colored substances to change water color to block light. This method is poor in effect and may affect the cultivation. And secondly, a chemical substance killing method, which is mainly a chemical herbicide, has quick response but has risks and hidden dangers for cultivation, and the cultured animals and aquatic weeds can die due to improper treatment. And thirdly, a nutrition competition method, which is mainly characterized in that microorganisms compete for nutrient elements such as nitrogen and phosphorus, so that the growth of the moss is limited due to lack of nutrition. The method is safe, but has long action time and slow effect. Therefore, there is an urgent need for aquaculture to develop a compound preparation which is safe for aquaculture organisms and aquatic weeds, can rapidly block the nutrient supply of moss, destroy the cell structure of moss, cut off the photosynthetic pathway of moss, inhibit the growth of moss and eliminate the harm of moss to aquaculture.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a compound preparation for inhibiting the growth of moss in an aquaculture pond, which integrates the characteristics of rapid direct reaching inside moss cells, rapid action, obvious effect, safety to cultured organisms and ecology and the like, can be directly put into a culture water body, and has the advantages of small usage amount, long storage period, high activity and convenient use.
In order to achieve the technical purpose, the technical scheme of the invention is as follows:
a composite preparation for inhibiting the growth of moss in an aquaculture pond comprises the following components in parts by mass:
0.1-25 parts of cellulase, 0.1-35 parts of pectinase, 0.1-75 parts of bacillus subtilis powder, 25-125 parts of lanthanum oxide, 1-55 parts of sodium lauryl sulfate, 85-250 parts of citric acid and 900 parts of anhydrous sodium sulfate.
The composite preparation is uniformly mixed by adopting a stirring mode.
The enzyme activity of the cellulase is 10 ten thousand U/g.
The enzyme activity of the pectinase is 20 ten thousand U/g.
The viable bacteria content of the bacillus subtilis powder is 1000 hundred million/g.
The dosage of the compound preparation is 800-1000g per mu of water surface and per meter of water depth.
The action principle of each component of the invention is as follows:
cellulase: cellulase (beta-1, 4-glucan-4-glucan hydrolase) is a general name of a group of enzymes for degrading cellulose to generate glucose, is not a monomer enzyme, is a multi-component enzyme system with a synergistic effect, is a complex enzyme, mainly comprises exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, and also has xylanase with high activity. Which act on cellulose and on products derived from cellulose. The optimum pH of the cellulase is generally 4.5-6.5. The invention breaks the cell wall of the moss by using the moss, destroys the cell structure of the moss and is convenient for other substances to permeate.
And (3) pectinase: pectinase is an enzyme that breaks down the pectic substance, the main component of plants. Pectinase is widely distributed in higher plants and microorganisms, and can degrade the pectic substance between cells and separate cells from tissues. Pectinases are included in two classes, one of which catalyzes the depolymerization of pectin and the other of which catalyzes the hydrolysis of esters in the pectin molecule. The enzymes catalyzing depolymerization of pectic substances are classified into enzymes acting on pectin (polymethylgalactose, aldolase, aldehydic acid lyase, or pectin lyase) and enzymes acting on pectic acid (polygalacturonase, polygalacturonic acid lyase, or pectate lyase). The enzymes catalyzing the hydrolysis of esters in pectin molecules are pectinesterase and pectoyl hydrolase. The invention utilizes the green moss to break the green moss cells, free chloroplasts, separate magnesium from chlorophyll and block the photosynthesis of the green moss.
B, bacillus subtilis powder: the Bacillus subtilis is one of Bacillus, has a single cell of 0.7-0.8 multiplied by 2-3 microns, is uniformly colored, has no capsule, is provided with periphytic flagellum, and can move. Gram-positive bacteria can form endogenous antipodal spores, spores are 0.6-0.9 multiplied by 1.0-1.5 microns, the spores are oval to columnar and are positioned in the center of the bacteria or slightly deviated from the center, and the bacteria do not expand after the spores are formed. The growth and propagation speed is high, the surface of a colony is rough and opaque, and is white or yellowish, and when the colony grows in a liquid culture medium, the skin becomes always formed, so that the colony is an aerobic bacterium. It can utilize protein, various sugars and starch to decompose tryptophan to form indole. The competitive action of the bacillus subtilis mainly comprises two aspects of nutrition competition and space site competition. The invention utilizes the nitrogen and phosphorus nutrition competition of the moss to slow down the growth of the moss.
Lanthanum oxide: lanthanum oxide is an oxide of the rare earth element lanthanum, and the molecular formula is La2O3. Slightly soluble in water, easily soluble in acid, and capable of forming insoluble matter with phosphorus in water to remove phosphorus. The invention utilizes the organic phosphorus to reduce the phosphorus content in the water body around the moss and inhibit the growth of the moss.
Sodium lauryl sulfate: sodium dodecyl sulfate, an organic substance, of the formula C12H25SO4Na, white or light yellow powder, is soluble in water and is not sensitive to alkali and hard water. Has the advantages of decontamination, emulsification, excellent foaming power and biological degradation>90 percent of the total cationic surfactant can be widely applied to industries such as cosmetics, detergents, textile, papermaking, lubrication, pharmacy, building materials, chemical engineering, oil extraction and the like, and can also be applied to basic research aspects such as the property of a positive and negative ionic surfactant compound system, micelle catalysis, molecular ordered combination and the like. Sodium dodecyl sulfate is a commonly used ionic detergent that disintegrates cell membranes and binds to the hydrophobic portion of membrane proteinsAnd the protein is separated from the membrane, and the high-concentration SDS can also destroy non-covalent bonds such as ionic bonds, hydrogen bonds and the like in the protein and even change the conformation of the protein. The invention utilizes the surface activity to promote the degradation inside and outside the moss cell and promote the penetration of substances.
Citric acid: citric acid, also known as citric acid, is an important organic acid, is a colorless crystal, odorless, strongly sour, readily soluble in water, and is a natural preservative and food additive. The invention provides an acidic action environment for 2 enzymes, and the complex magnesium element destroys the chlorophyll structure and blocks the photosynthesis.
Anhydrous sodium sulfate: the anhydrous sodium sulfate is salt formed by combining sulfate radical and sodium ion, and has a chemical formula of Na2SO4It is soluble in water, and the solution is mostly neutral. The anhydrous substance with high purity and fine particles is called anhydrous sodium sulphate, has hygroscopicity, and is mainly used for manufacturing water glass, enamel, paper pulp, refrigerating mixing agent, detergent, drying agent, dye diluent, analytical chemical reagent, medicinal products, feed and the like. The present invention utilizes it as a carrier and a moisture absorbent.
From the above description, it can be seen that the present invention has the following advantages:
1. the invention integrates the characteristics of rapid direct reaching into moss cells, rapid action, obvious effect, safety to cultivated organisms and ecology and the like, can be directly put into a cultivation water body, and has the advantages of small usage amount, long storage period, high activity and convenient use.
2. The preparation prepared by the invention can rapidly block the nutrition supply of the moss, destroy the cell structure of the moss, cut off the photosynthetic pathway of the moss, inhibit the growth of the moss and eliminate the harm of the moss to aquaculture, thereby achieving the purposes of improving the ecological environment of aquaculture and improving the aquaculture yield, quality and benefit; in the using process, the preparation quickly reaches the surface of the moss cells, directly destroys cell walls and cell membranes, releases blocking factors into the cells, acts on chloroplasts, cuts off photosynthetic pathways of the moss, reduces and complexes nitrogen and phosphorus nutrient elements, and blocks nutrient supply of the moss, thereby quickly and effectively inhibiting growth of the moss and ensuring safety and ecological stability of cultured animals.
Detailed Description
The present invention is described in detail with reference to examples, but the present invention is not limited to the claims.
Example 1
A composite preparation for inhibiting the growth of moss in an aquaculture pond comprises the following components in parts by weight: 20 parts of cellulase, 30 parts of pectinase, 70 parts of bacillus subtilis powder, 100 parts of lanthanum oxide, 50 parts of sodium lauryl sulfate, 200 parts of citric acid and 530 parts of anhydrous sodium sulfate; uniformly mixing the raw materials to obtain a composite preparation for inhibiting the growth of moss in an aquaculture pond, wherein the enzyme activity of the cellulase is 10 ten thousand U/g; the enzyme activity of the pectinase is 20 ten thousand U/g; the viable bacteria content of the bacillus subtilis powder is 1000 hundred million/g.
Example 2
A composite preparation for inhibiting the growth of moss in an aquaculture pond comprises the following components in parts by weight: 22 parts of cellulase, 33 parts of pectinase, 60 parts of bacillus subtilis powder, 90 parts of lanthanum oxide, 45 parts of sodium lauryl sulfate, 210 parts of citric acid and 540 parts of anhydrous sodium sulfate; uniformly mixing the raw materials to obtain a composite preparation for inhibiting the growth of moss in an aquaculture pond, wherein the enzyme activity of the cellulase is 10 ten thousand U/g; the enzyme activity of the pectinase is 20 ten thousand U/g; the viable bacteria content of the bacillus subtilis powder is 1000 hundred million/g.
Example 3
A composite preparation for inhibiting the growth of moss in an aquaculture pond comprises the following components in parts by weight: 18 parts of cellulase, 35 parts of pectinase, 52 parts of bacillus subtilis powder, 110 parts of lanthanum oxide, 50 parts of sodium lauryl sulfate, 200 parts of citric acid and 535 parts of anhydrous sodium sulfate; uniformly mixing the raw materials to obtain a composite preparation for inhibiting the growth of moss in an aquaculture pond, wherein the enzyme activity of the cellulase is 10 ten thousand U/g; the enzyme activity of the pectinase is 20 ten thousand U/g; the viable bacteria content of the bacillus subtilis powder is 1000 hundred million/g.
Application example 1
The area of the river crab culture pond in the Yixing area of Jiangsu is 8 mu, the water depth is 0.6 m, the density of phytoplankton in the pond in the early culture period is low, the water is clear, and the bottom of the pond is provided with a piece of moss, which influences the growth of river crabs and waterweeds.
The composite preparation for inhibiting the growth of moss in an aquaculture pond prepared in example 1 is applied to a river crab aquaculture pond in Yixing areas of Jiangsu, the dosage of the composite preparation is 500g per mu of water depth of 0.6 m, and the composite preparation is uniformly put in a moss growth area. 3 days after use, the moss turns yellow and floats. The moss at the bottom of the pool is basically atrophied after 8 days of use, the moss is used once with the same dosage at the moment, and the toxicity and the water are detoxified and fertilized 12 days later, so that the water body fertility is good, and the river crabs grow healthily.
Application example 2
The area of the river crab culture pond in Jiangsu Suzhou area is 10 mu, the water depth is 0.5 m, the density of phytoplankton in the pond in the early culture period is low, the water is clear, and the bottom of the pond is provided with a piece of moss, so that the growth of river crabs and waterweeds is influenced.
The composite preparation for inhibiting the growth of moss in an aquaculture pond prepared in example 2 is applied to a river crab aquaculture pond in Yixing areas of Jiangsu, the dosage of the composite preparation is 400g per mu in a water depth of 0.5 m, and the composite preparation is uniformly put in a moss growth area. 3 days after use, the moss turns yellow and floats. The moss at the bottom of the pool is basically atrophied after 7 days of use, the moss is used once with the same dosage at the moment, and the toxicity and the water are detoxified and fertilized after 10 days, so that the water body fertility is good, and the river crabs grow healthily.
Application example 3
The area of the river crab culture pond in the high pure and pure region of Jiangsu is 12 mu, the water depth is 0.7 m, the density of phytoplankton in the pond in the early culture period is low, the water is clear, and the bottom of the pond is provided with a piece of moss which influences the growth of river crabs and waterweeds.
The composite preparation for inhibiting the growth of moss in an aquaculture pond prepared in example 3 is applied to a river crab aquaculture pond in Yixing areas of Jiangsu, the dosage of the composite preparation is 700g per mu in a water depth of 0.7 m, and the composite preparation is uniformly put in a moss growth area. 3 days after use, the moss turns yellow and floats. The moss at the bottom of the pool is basically atrophied after being used for 6 days, the moss is used once with the same dosage at the moment, and the toxicity and the water are removed and the water fertility are good after 10 days, so that the river crabs grow healthily.
It should be understood that the detailed description of the invention is merely illustrative of the invention and is not intended to limit the invention to the specific embodiments described. It will be appreciated by those skilled in the art that the present invention may be modified or substituted equally as well to achieve the same technical result; as long as the use requirements are met, the method is within the protection scope of the invention.
Claims (6)
1. A composite preparation for inhibiting the growth of moss in an aquaculture pond, which is characterized in that: the mass ratio is as follows:
0.1-25 parts of cellulase, 0.1-35 parts of pectinase, 0.1-75 parts of bacillus subtilis powder, 25-125 parts of lanthanum oxide, 1-55 parts of sodium lauryl sulfate, 85-250 parts of citric acid and 900 parts of anhydrous sodium sulfate.
2. The complex formulation for inhibiting moss growth in aquaculture ponds according to claim 1, wherein: the composite preparation is uniformly mixed by adopting a stirring mode.
3. The complex formulation for inhibiting moss growth in aquaculture ponds according to claim 1, wherein: the enzyme activity of the cellulase is 10 ten thousand U/g.
4. The complex formulation for inhibiting moss growth in aquaculture ponds according to claim 1, wherein: the enzyme activity of the pectinase is 20 ten thousand U/g.
5. The complex formulation for inhibiting moss growth in aquaculture ponds according to claim 1, wherein: the viable bacteria content of the bacillus subtilis powder is 1000 hundred million/g.
6. The complex formulation for inhibiting moss growth in aquaculture ponds according to claim 1, wherein: the dosage of the compound preparation is 800-1000g per mu of water surface and per meter of water depth.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011508842.XA CN112777752A (en) | 2020-12-18 | 2020-12-18 | Composite preparation for inhibiting growth of moss in aquaculture pond and application |
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| CN202011508842.XA CN112777752A (en) | 2020-12-18 | 2020-12-18 | Composite preparation for inhibiting growth of moss in aquaculture pond and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114617135A (en) * | 2022-03-14 | 2022-06-14 | 中国林业科学研究院林产化学工业研究所 | Biological agent with moss deactivating effect and preparation method and application thereof |
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| CN103241816A (en) * | 2013-05-24 | 2013-08-14 | 无锡中顺生物技术有限公司 | Cleaning method of aquatic weeds in river crab aquaculture pond |
| CN105217802A (en) * | 2015-10-29 | 2016-01-06 | 余姜 | A kind of water quality cleansing agent |
| CN106315869A (en) * | 2016-10-28 | 2017-01-11 | 中国水产科学研究院淡水渔业研究中心 | Moss ecological control agent for aquaculture and application thereof |
| CN107376834A (en) * | 2017-08-31 | 2017-11-24 | 常州富思通管道有限公司 | A kind of preparation method for the fishing purse seine for handling breeding pollution |
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2020
- 2020-12-18 CN CN202011508842.XA patent/CN112777752A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101343090A (en) * | 2008-08-22 | 2009-01-14 | 王德林 | Lanthanide series water treatment agent and method of preparing the same |
| CN103241816A (en) * | 2013-05-24 | 2013-08-14 | 无锡中顺生物技术有限公司 | Cleaning method of aquatic weeds in river crab aquaculture pond |
| CN105217802A (en) * | 2015-10-29 | 2016-01-06 | 余姜 | A kind of water quality cleansing agent |
| CN106315869A (en) * | 2016-10-28 | 2017-01-11 | 中国水产科学研究院淡水渔业研究中心 | Moss ecological control agent for aquaculture and application thereof |
| CN107376834A (en) * | 2017-08-31 | 2017-11-24 | 常州富思通管道有限公司 | A kind of preparation method for the fishing purse seine for handling breeding pollution |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114617135A (en) * | 2022-03-14 | 2022-06-14 | 中国林业科学研究院林产化学工业研究所 | Biological agent with moss deactivating effect and preparation method and application thereof |
| CN114617135B (en) * | 2022-03-14 | 2024-04-26 | 中国林业科学研究院林产化学工业研究所 | Biological agent with green removing function and preparation method and application thereof |
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