CN105441343A - Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof - Google Patents
Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof Download PDFInfo
- Publication number
- CN105441343A CN105441343A CN201510824786.3A CN201510824786A CN105441343A CN 105441343 A CN105441343 A CN 105441343A CN 201510824786 A CN201510824786 A CN 201510824786A CN 105441343 A CN105441343 A CN 105441343A
- Authority
- CN
- China
- Prior art keywords
- saccharomyces cerevisiae
- yeast
- culture
- fermentation
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses Saccharomyces cerevisiae JI1401 which is separated from sour dough and has the preservation number of CGMCC No.11470, preparation of a Saccharomyces cerevisiae culture from the Saccharomyces cerevisiae as well as an application of the Saccharomyces cerevisiae culture to influence on growth performance of nursery pigs. The strain is separated from the sour dough, and the Saccharomyces cerevisiae culture is prepared through combination of traditional herbal leaven and the modern fermentation technology. The Saccharomyces cerevisiae culture contains multiple active substances and has effects of obviously promoting propagation of beneficial microorganisms of piglet intestines and decreasing the diarrhea rate.
Description
Technical field
The yeast saccharomyces cerevisiae that the present invention relates to biological technical field, particularly regulate host immune function, improve enteral nutritional support and colony balance, improve nutrient utilization.
Background technology
Yeast culture is a kind of Tiny ecosystem goods made after sufficient anaerobically fermenting drying on special substratum by yeast under specific process conditions.It is made up of yeast cell meta-bolites, the substratum made a variation afterwards by fermentation and a small amount of yeast cell, nutritious.Yeast culture prebiotic effect is mainly manifested in and regulates host immune function, improves enteral nutritional support and colony balance, improves nutrient utilization, and then promotes growth of animal performance.
Harrison, the high numerous investigator of week connection have confirmed that yeast culture has good effect to ruminant tumor gastric fermentation, production performance all.The mechanism of action of yeast culture is different from simple yeast preparation, and the quality of its action effect depends primarily on the quality of bacterial classification, and excellent bacterial classification can make active substance in culture abundanter, thus effect is more obvious.The widely different degree of yeast product action effect depending on the difference of bacterial strain uses therefor, is secondly zymotechnique.Excellent yeast strain is the basis of Ruminat s Microecological Agent research and apply.In traditional herbal leaven Divine Comedy and sour flour dough, yeast is main probiotics.Lixia ZHANGs etc. are separated 4 Yeasts in intensive song, wherein have yeast saccharomyces cerevisiae.Yang Xuejuan etc. are separated to the excellent Saccharomyces Cerevisiae in S y22 of a strain from sour flour dough, can bread dough separately.The bacterial classification that these spontaneous fermentations occur is applied in the preparation of modern yeast culture, not only there is certain science innovation meaning, and provide some to help and thinkings for the research and development of domestic probiotics, development of new yeast culture, is significant to China's feed additive field.
Summary of the invention
An object of the present invention is yeast saccharomyces cerevisiae JI1401, and utilize the novel bacterial be separated to prepare a kind of composite yeast culture.
Yeast JI1401 of the present invention is separated to obtain from sour flour dough, and yeast JI1401 bacterium colony is creamy white, smooth surface, neat in edge.Cell is circular or oval, single-endedly sprouts, without pseudohypha, and 1 ~ 4 thecaspore.Described yeast has typical yeast saccharomyces cerevisiae biochemical character: can glucose fermentation, sucrose, maltose, semi-lactosi, raffinose; Nonfermented lactose, trehalose and Zulkovsky starch.Glucose, sucrose, maltose, semi-lactosi can be assimilated, do not assimilate lactose, cellobiose, citric acid, wood sugar, sorbyl alcohol, N.F,USP MANNITOL, inositol, pectinose, succsinic acid, Zulkovsky starch.(NH4) 2SO4 can be assimilated, do not assimilate KNO3.
This yeast saccharomyces cerevisiae (Saccharomycescerevisiae) bacterial strain JI1401, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 30th, 2015, and preserving number is: CGMCCNo.11470. depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
The preparation method of the yeast culture adopting yeast saccharomyces cerevisiae JI1401 to prepare, comprises following technique:
1) seed liquor preparation: the yeast saccharomyces cerevisiae be separated to is inoculated in liquid nutrient medium, obtains seed liquor.
2) liquid aerobic expands numerous fermentation: seed liquor be inoculated in liquid nutrient medium, carries out aerobic and expands numerous, obtain liquid fermenting solution.
3) solid anaerobic fermentation: liquid fermentation liquid is inoculated on solid medium, anaerobically fermenting, obtains culture.
4) by solid culture natural air drying, pulverize, obtain yeast culture.
Described 1) seed liquor substratum is YPD substratum, i.e. glucose 20g, peptone 10g, yeast extract paste 10g, is dissolved in 1L water, natural PH, 115 DEG C of sterilizing 20min.Described 2) liquid nutrient medium is malt juice liquid medium, buys in Beijing De Tianzhicheng Science and Technology Ltd..Described 3) solid medium is flour 30g, wheat bran 70g, dregs of beans 2g.
Another object of the present invention is to provide the application of above-mentioned yeast culture in child care pig produces.
Experiment shows: yeast culture of the present invention can improve day weight gain and food consumption, reduces feed-weight ratio, significantly improves energy and dry-matter digestibility.Significantly improve enteron aisle lactic acid bacterium number and reduce intestinal bacteria quantity, grice diarrhoea rate is significantly reduced.Illustrate that this yeast culture as the effective probiotics of one, to maintain intestinal microbial balance, can play growth promoting function.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1
Of the present inventionly be ordinary method, reagent is all obtained by commercial sources.
The acquisition of yeast saccharomyces cerevisiae JI1401
(1) Isolation and screening of yeast saccharomyces cerevisiae
Sour flour dough 10g is added respectively in 200mlYPD liquid nutrient medium, after the lower 30 DEG C of cultivation 24h of aseptic condition, shake up, use stroke-physiological saline solution gradient dilution, choose 10-6, 10-7, 10-83 extent of dilution, accurate absorption 0.1ml diluent is spread evenly across on YPD solid medium, 48h is cultivated in 30 DEG C of constant incubators, from herbal leaven, the single bacterium colony of the typical case of a kind of similar yeast saccharomyces cerevisiae of picking is in YPD liquid nutrient medium, to rule after cultivating 24h at 30 DEG C separation 2 ~ 3 times, pure growth is turned after 12h cultivated by YPD solid slant culture base, be placed in 4 DEG C of cryogenic refrigerator preservations.
(2) qualification of bacterial classification
Biochemical identification: be sub-packed in by 12.5% bean sprout juice in the test tube containing Du Shi tubule, after sterilizing, adding wherein through boiling to obtain 10% liquid glucose, making sugar concentration reach 2%.Access in fermentation tube with transfering loop by bacterial classification again, often kind of sugar three is parallel, with not sugaring, does not connect the test tube of bacterium for blank, 30 DEG C of cultivations, observes bubbles volume in test tube.Utilize without carbon source basic medium with without nitrogenous source basic medium, pour bacteria suspension into, pour in culture dish after mixing, after to be solidified, test carbon source is put its surface, cultivate to observe around carbon source whether form growth circle for 30 DEG C.Result shows, and the yeast be separated to can glucose fermentation, sucrose, maltose, semi-lactosi, raffinose; Nonfermented lactose, trehalose and Zulkovsky starch.Glucose, sucrose, maltose, semi-lactosi can be assimilated, do not assimilate lactose, cellobiose, citric acid, wood sugar, sorbyl alcohol, N.F,USP MANNITOL, inositol, pectinose, succsinic acid, Zulkovsky starch.(NH4) 2SO4 can be assimilated, do not assimilate KNO3.According to sugar-fermenting, the qualification of carbon nitrogen source assimilation experiments, can preliminary judgement yeast be yeast saccharomyces cerevisiae, called after JI1401.
Molecular Identification: adopt pearl mill method to carry out cell wall breaking and extract DNA, with the DNA of preparation for template, ITS15 '-TCCGTAGGTGAACCTGCGG-3 ', ITS45 '-TCCTCCGCTTATTGATATGC-3 is primer, carry out pcr amplification, reaction system is 50uL:10 × Buffer5 μ L, dNTP4 μ L, MgCl24 μ L, each 1 μ L of primer (10 μMs), template DNA 40-50ng, ExTag (Takara, Dalian) 1.5U, sterilizing ultrapure water polishing to 50uL, reaction conditions is 95 DEG C of 5min; 95 DEG C of 1min, 55 DEG C of 1min, 72 DEG C of 1.5min, 35 circulations; 72 DEG C of 10min.Agarose gel electrophoresis is utilized to detect amplified production, amplified fragments is about about 850bp, ncbi database (nucleotidecollection) is used to carry out blast comparison to amplification PCR, draw comparison result, display JI1401 and yeast saccharomyces cerevisiae homology reach 99%, can be defined as yeast saccharomyces cerevisiae.
Above-mentioned experimental result shows, the bacterial classification JI1401 be separated to is yeast saccharomyces cerevisiae.
Embodiment 2
The preparation of yeast culture
Prepared by seed liquor: the YPD substratum of preparation 500ml distilled water, 10g glucose, 5g peptone, 5g yeast extract paste composition, nature PH, packing two parts, 115 DEG C of sterilizing 20min, then the good JI1401 bacterial strain of purifying is inoculated in liquid nutrient medium, 30 DEG C, 12h cultivated by 200r/min shaking table, obtains seed liquor.
Liquid aerobic expands numerous: first bottledly have the malt juice liquid medium of 50ml/250ml in 121 DEG C of sterilizing 20min by two, afterwards according to 5% addition, in step 1 two kind of seed liquor is added in malt extract medium respectively and ferments, 30 DEG C, 200r/min shaker fermentation 24h.
Solid anaerobic fermentation: 70g wheat bran, 30g flour, 2g dregs of beans are poured in 1L Erlenmeyer flask, adding distilled water makes water content reach 60%, mix rear 121 DEG C of sterilizing 20min, after cooling, addition by 10%, by fermented liquid obtained in step 2, is added in solid medium, stirs with sterile glass rod, sealing bottleneck, is put in 30 DEG C of standing for fermentation 48h.
Obtained yeast culture is evenly fallen apart in porcelain dish, is put in room temperature, natural air drying.Pulverize 40-60 mesh sieve, be composite yeast culture.
Embodiment 3
Yeast culture is applied the impact of piglet growth performance
Select healthy, that mean body weight be (15.7 ± 0.26) kg " Du × long × large " three way cross growing swine 90, the principle basically identical by body weight, sex is divided into 2 groups, often group 3 repetitions, each repetition 15 at random.Control group fed basal diet; Yeast culture group is fed basal diet+5g/kgYC, and trial period is 35d.Testing index has average daily gain, average daily ingestion amount, feed-weight ratio, diarrhea rate, apparent digestibility and enteric microorganism.
Experimental result shows, adds YC and can improve average daily gain 7.2% in piglet diet, improves average daily ingestion amount 1.74%, reduces feed-weight ratio 5.45% (P > 0.05).Significantly improve the digestibility of piglet to energy and dry-matter, and significantly increase enteron aisle lactic acid bacterium number and reduce intestinal bacteria quantity, thus grice diarrhoea rate is significantly reduced (P < 0.05).
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this specification sheets is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should by specification sheets integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (5)
1. Saccharomyces Cerevisiae in S accharomycescerevisiae, bacterial strain JI1401, its preserving number is CGMCCNo.11470, is deposited in Chinese microorganism strain preservation center.
2. prepare a method for yeast culture, it is characterized in that, claim 1 yeast saccharomyces cerevisiae JI1401 is carried out liquid and expands numerous fermentation, and then solid anaerobic fermentation, last air-dry pulverization process.
3. preparation method according to claim 2, it is characterized in that, concrete preparation process is: the good JI1401 bacterial strain of inoculation purifying is in YPD liquid nutrient medium, 30 DEG C, 12h cultivated by 200r/min shaking table, obtain seed liquor, then according to 5% addition, seed liquor is added in malt extract medium and ferments, 30 DEG C, 200r/min shaker fermentation 24h, adds in solid medium by the addition of 10% by fermented liquid afterwards, stirs with sterile glass rod, sealing bottleneck, be put in 30 DEG C of standing for fermentation 48h, last natural air drying, pulverize.
4. preparation method according to claim 3, it is characterized in that, described YPD liquid nutrient medium preparation method is glucose 20g, peptone 10g, yeast extract paste 10g, be dissolved in 1L water, nature PH, 115 DEG C of sterilizing 20min, the proportioning of described solid medium is: flour 30g, wheat bran 70g, dregs of beans 2g.
5. the application of yeast culture in child care pig produces that claim 2 is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510824786.3A CN105441343A (en) | 2015-11-24 | 2015-11-24 | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510824786.3A CN105441343A (en) | 2015-11-24 | 2015-11-24 | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105441343A true CN105441343A (en) | 2016-03-30 |
Family
ID=55551987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510824786.3A Pending CN105441343A (en) | 2015-11-24 | 2015-11-24 | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105441343A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689819A (en) * | 2017-01-06 | 2017-05-24 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
| CN109321478A (en) * | 2018-11-17 | 2019-02-12 | 河南省科学院生物研究所有限责任公司 | It is a kind of degrade mycotoxin bacterial strain yk18 and its application |
| CN109401990A (en) * | 2018-09-25 | 2019-03-01 | 北京中农弘科生物技术有限公司 | One plant of S. cervisiae HKB-36 and its application with bacteriostatic activity |
| CN111903838A (en) * | 2020-08-13 | 2020-11-10 | 厦门惠盈动物科技有限公司 | Yeast culture and compound lactobacillus preparation and preparation method thereof |
| KR102410918B1 (en) * | 2021-07-29 | 2022-06-23 | 주식회사 르빵 | Solid medium composition comprising cereal and method for producing dry yeast using the same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463329A (en) * | 2009-01-07 | 2009-06-24 | 北京龙科方舟生物工程技术中心 | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof |
| CN102936572A (en) * | 2012-09-19 | 2013-02-20 | 北京英惠尔生物技术有限公司 | Acid resistance and high temperature resistance saccharomyces cerevisiae and applications thereof |
| CN103074240A (en) * | 2012-12-06 | 2013-05-01 | 北京中科景明生物技术有限公司 | Yeast for high-activity feed, and its application |
-
2015
- 2015-11-24 CN CN201510824786.3A patent/CN105441343A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463329A (en) * | 2009-01-07 | 2009-06-24 | 北京龙科方舟生物工程技术中心 | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof |
| CN102936572A (en) * | 2012-09-19 | 2013-02-20 | 北京英惠尔生物技术有限公司 | Acid resistance and high temperature resistance saccharomyces cerevisiae and applications thereof |
| CN103074240A (en) * | 2012-12-06 | 2013-05-01 | 北京中科景明生物技术有限公司 | Yeast for high-activity feed, and its application |
Non-Patent Citations (1)
| Title |
|---|
| 潘宝海等: "酿酒酵母对仔猪生产性能和消化道微生物区系的影响", 《饲料研究》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689819A (en) * | 2017-01-06 | 2017-05-24 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
| CN109401990A (en) * | 2018-09-25 | 2019-03-01 | 北京中农弘科生物技术有限公司 | One plant of S. cervisiae HKB-36 and its application with bacteriostatic activity |
| CN109401990B (en) * | 2018-09-25 | 2022-02-15 | 北京中农弘科生物技术有限公司 | Saccharomyces cerevisiae HKB-36 with bacteriostatic activity and application thereof |
| CN109321478A (en) * | 2018-11-17 | 2019-02-12 | 河南省科学院生物研究所有限责任公司 | It is a kind of degrade mycotoxin bacterial strain yk18 and its application |
| CN109321478B (en) * | 2018-11-17 | 2021-07-16 | 河南省科学院生物研究所有限责任公司 | Strain yk18 for degrading mycotoxin and application thereof |
| CN111903838A (en) * | 2020-08-13 | 2020-11-10 | 厦门惠盈动物科技有限公司 | Yeast culture and compound lactobacillus preparation and preparation method thereof |
| KR102410918B1 (en) * | 2021-07-29 | 2022-06-23 | 주식회사 르빵 | Solid medium composition comprising cereal and method for producing dry yeast using the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104996722B (en) | A kind of method of the step combined ferment feed of multi-cultur es two | |
| CN105441343A (en) | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof | |
| CN101422249B (en) | Method for promoting calcium transformation ratio increasing in sheep bone using enzymolysis and fermentation | |
| CN103911323A (en) | Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation | |
| CN104664154B (en) | Yeast culture and preparation method thereof | |
| CN105432935A (en) | Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry | |
| CN107937292A (en) | A kind of Cultures of S. cerevisiae and its zymotechnique | |
| CN103468604B (en) | Bacillus subtilis and application thereof | |
| CN102093958B (en) | Method for producing ultrathin fermentation bed bacteria | |
| CN102559560B (en) | Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis | |
| CN103074284A (en) | Zymophyte liquid, liquid spirit stillage fermented feed and fermentation method of liquid spirit stillage fermented feed | |
| CN102140427A (en) | Method for preparing intensified daqu applied to nongjiang-flavor Chinese spirits | |
| CN115287202B (en) | Kluyveromyces marxianus and application thereof in preparation of functional feed | |
| CN109022333A (en) | A kind of preparation method and applications of composite microbial fermentation bacteria agent | |
| CN104164459A (en) | Method utilizing fermentation to improve gamma-aminobutyric acid content of brown rice | |
| CN106722082A (en) | A kind of high dietary-fiber wheat bran red date probiotics chewable tablets | |
| CN103468594A (en) | Candidautilis strain and application thereof | |
| CN109666599A (en) | A kind of lactobacillus reuteri high density fermentation culture medium and fermentation process, application | |
| CN106906160A (en) | A kind of synergist and its fermentation process for lactobacillus-fermented | |
| CN109259188A (en) | A kind of preparation method of the wheat bran lactic fermentation piece rich in viable bacteria | |
| CN113549574A (en) | Bacillus coagulans and application thereof | |
| CN102168029B (en) | Bacilluslicheniformis and application thereof | |
| CN105087421B (en) | The method that yeast fusion bacterium mixes microbial inoculum and preparation method thereof and production organic fertilizer | |
| CN104745503A (en) | Bacillus subtilis and application of bacillus subtilis in simple and elegant liquor | |
| CN104630182B (en) | A kind of grower pigs compound enzyme and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160330 |