CN109401990A - One plant of S. cervisiae HKB-36 and its application with bacteriostatic activity - Google Patents
One plant of S. cervisiae HKB-36 and its application with bacteriostatic activity Download PDFInfo
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- CN109401990A CN109401990A CN201811116994.8A CN201811116994A CN109401990A CN 109401990 A CN109401990 A CN 109401990A CN 201811116994 A CN201811116994 A CN 201811116994A CN 109401990 A CN109401990 A CN 109401990A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
Abstract
The present invention relates to an Accharomyces cerevisiae bacterium (Saccharomyces cerevisiae) HKB-36 and its applications, wherein S. cervisiae HKB-36, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on November 8th, 2016, it is referred to as CGMCC, and deposit number is CGMCC No.13261.S. cervisiae HKB-36 of the invention, S. cervisiae HKB-36 provided by the invention has the characteristics that growth metabolism ability is vigorous, fermentating metabolism object has bacteriostatic activity, the strain fermentating liquid of 200 μ L, its inhibition zone diameter can reach 7mm, the production that can be applied to novel fodder raw material yeast culture, makes an addition in animal and fowl fodder, inhibits harmful microbe growth in animal and bird intestines, improve the intestinal health of livestock and poultry and improve growth performance, reduces the use of antibiotic in feed.
Description
Technical field
The present invention relates to fungi field, in particular to one plant has the S. cervisiae HKB-36 of bacteriostatic activity and its answers
With.
Background technique
Saccharomyces cerevisiae, also known as Saccharomyces cerevisiae or budding yeast belong to the Blastocystis section member in Ascomycotina, are
A kind of eukaryotic microorganisms that cellular prion protein is fairly simple, while being a primary yeast most close with human relation.Early in
1963, Bevan etc. found in the brewer's yeast of preservation for the first time it is a kind of with antibacterial/bacteriostatic activity saccharomycete, in its life
A kind of toxin protein can be outwardly secreted in long reproductive process, this toxin protein can kill or inhibit other microorganisms.With
Going deep into for research, discovery antibacterial type yeast there are many purposes, fermentation, medicine, environment, food and in terms of development before
Scape is wide.
Have the history of many years using saccharomyces cerevisiae source biological feed in livestock-raising, but livestock application in the past
Mostly yeast single cell protein or preliminary working product, underuse the high added value of yeast.It is even disabled as China limits
The development of fodder antibiotics action, such as agricultural in April, 2018 rural area portion disclose " Antibiotic use minimizing action pilot for animals
Programme of work (2018-2021), scheme are pointed out to strive to implement cultivating link Antibiotic use for animals by 3 years and subtract
Quantization action pilot work, medicated feed additive will all be exited in the year two thousand twenty.This will produce China's animal husbandry and imitate
Rate is a large impact, and sufficiently exploitation can promote livestock birds health level, the feed resource of raising production performance extremely urgent, this
Under form, have saccharomyces cerevisiae and its biological products with important application prospects.
But in the prior art, saccharomyces cerevisiae and its biological products emphasize the height of its protein content more, also not related
In the report of the antibacterial biological products of yeast.How to make full use of saccharomyces cerevisiae, improve the antibacterial biological products of yeast quality and its
Function and effect, and reducing its production cost is current studies in China urgent problem to be solved.
Summary of the invention
The purpose of the present invention is in order to solve the above problem, the present invention provides one plant of S. cervisiae with bacteriostatic activity
HKB-36 and its application.
According to the first aspect of the invention, one plant of S. cervisiae (Saccharomyces with bacteriostatic activity is provided
Cerevisiae) HKB-36 is preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life on November 8th, 2016
Object center, is referred to as CGMCC, and deposit number is CGMCC No.13261.
According to the second aspect of the invention, a kind of fermentating metabolism object is provided, which is the S. cervisiae
The aerobic fermentation metabolite of HKB-36.
According to the third aspect of the invention we, a kind of antibacterial biological products are provided, which includes the fermentating metabolism object.
According to the fourth aspect of the invention, the preparation method of the fermentating metabolism object is provided, which includes aerobic hair
Ferment step:
Wherein, fluid nutrient medium includes that the malt of 145g/L leaches the chloramphenicol of powder and 0.08g/L, fluid nutrient medium pH
Value is 5.8-6.8；Fermentation temperature is 28 DEG C -36 DEG C, fermentation time 24-36h, ventilatory capacity 1-4L/min.
Wherein, preferably fermentation temperature is 32 DEG C, and the pH value of fluid nutrient medium is 6.4.
Wherein, preparation method further includes the seed culture step before aerobic fermentation step:
Fluid nutrient medium includes that the malt of 145g/L leaches the chloramphenicol of powder and 0.08g/L, and fluid nutrient medium pH value is
5.8-6.8；Cultivation temperature is 28 DEG C of -36 DEG C of constant-temperature table cultures, and incubation time is 18~30h, revolving speed 120-200r/min.
Wherein, in aerobic fermentation step, the inoculum concentration of seed liquor is 10%~20%.
According to the fourth aspect of the invention, the antibacterial biological products are provided, which includes slant strains activation, kind
Sub- liquid preparation, liquid aerobic fermentation and solid anaerobic fermentation step:
Wherein, in solid anaerobic fermentation step, fermentation medium includes 2-4 parts of vinasse, 2-4 parts of maize peel, 1-3 parts of wheat bran
With 1-3 parts of apple pomace, culture medium carbon-nitrogen ratio be 2-7:1, moisture content 35%-70%；Fermentation temperature is 28 DEG C -36 DEG C, hair
Ferment time 18-48h.
S. cervisiae HKB-36 provided by the invention is vigorous with growth metabolism ability, fermentating metabolism object has antibacterial work
The features such as property, the strain fermentating liquid of 200 μ L, inhibition zone diameter can reach 7mm.S. cervisiae HKB-36 can be applied to
The production of novel fodder raw material yeast culture, makes an addition in animal and fowl fodder, inhibits harmful microbe growth in animal and bird intestines,
It improves the intestinal health of livestock and poultry and improves growth performance, reduce the use of antibiotic in feed.
Detailed description of the invention
By reading the following detailed description of the preferred embodiment, various other advantages and benefits are common for this field
Technical staff will become clear.The drawings are only for the purpose of illustrating a preferred embodiment, and is not considered as to the present invention
Limitation.And throughout the drawings, the same reference numbers will be used to refer to the same parts.In the accompanying drawings:
Fig. 1 shows the growth curve chart of the S. cervisiae HKB-36 of embodiment according to the present invention.
Fig. 2 shows the colonial morphology electron microscopic pictures of the S. cervisiae HKB-36 of embodiment according to the present invention；
Fig. 3 shows the development tree graph piece of the S. cervisiae HKB-36 of embodiment according to the present invention；
Fig. 4 shows the fungistatic effect figure of the fermentating metabolism product of the different S. cervisiaes of embodiment according to the present invention
Piece；
Fig. 5 shows the fungistatic effect figure of the antibacterial biological products of the different S. cervisiaes of embodiment according to the present invention
Piece.
Specific embodiment
The illustrative embodiments of the disclosure are more fully described below with reference to accompanying drawings.Although showing this public affairs in attached drawing
The illustrative embodiments opened, it being understood, however, that may be realized in various forms the disclosure without the reality that should be illustrated here
The mode of applying is limited.It is to be able to thoroughly understand the disclosure on the contrary, providing these embodiments, and can be by this public affairs
The range opened is fully disclosed to those skilled in the art.
The separation screening of 1 S. cervisiae HKB-36 of embodiment
(1) culture medium: malt extract medium.500g malt is taken, is put into beaker after being crushed, 2000mL distillation is added
Water stirs lower 45 DEG C of water-bath 30min, and temperature is turned up to 70 DEG C, keeps 1h；8 layers of filtered through gauze, and with positive Liu Shu wash beaker and
Filter pocket makes filtrate reach 2000mL, after cooling, 10 DEG C save backup after boiling.
(2) separation of saccharomycete: with beer, grape wine, pickles, fermented fruits and vegetables juice, dough and koumiss etc. for raw material, point
Not Cai Ji 400mL or 400g material sample (smashing to pieces for solid), set in sterile conical flasks, 8 layers of gauze of lid in 25 DEG C -30 DEG C from
So fermentation 2-3d.Under aseptic condition, fermentation culture sample is shaken up, draws appropriate fermentation liquid in by going out with sterile pipette
In the brewer's wort culture solution of bacterium, 25 DEG C of cultures.Gradient dilution method is used to be diluted to concentration as 10 when having vinosity-4With 10-5, inhale
Culture solution after taking 0.1-0.2mL to dilute is spread evenly across on malt extract medium (+2% agar of brewer's wort culture solution), and 25 DEG C
It is inverted 48h, after growing bacterium colony, selection has typical single colonie (saccharomycete bacterium colony) further scribing line purifying 2-3 times, through microscopy
To be transferred in brewer's wort solid slope culture medium respectively after pure culture, 4 DEG C are saved backup.
(3) yeast screening assay: being cultivated by malt extract medium, observation period growing state, selects survival rate highest, life
Long most luxuriant bacterium carries out the bacteriostatic test screening of next step.Using Odontothrips loti, culture have Escherichia coli, salmonella,
It is separately added into the saccharomycete saved backup in the culture medium of staphylococcus glucose coccus, different saccharomycete are against three plants of harmful bacterias
Effect such as table 1 is killed in suppression, selects the optimal single bacterial strain of antibacterial/bacteriostatic activity, is named as HKB-36.
Table 1: different saccharomycete kill test to the suppression of Escherichia coli, salmonella and staphylococcus aureus
Growth metabolism ability and acid resistance measurement:
Different time points saccharomycete is observed with the malt extract medium culture HKB-36 of different pH value using fask oscillating method
The growing state of HKB-36, growth curve such as Fig. 1.HKB-36 just has reached when showing under optimum conditions less than 20min
Grow stationary phase, and Medium's PH Value be 3,4,5,6 it is lower can good growth, only just show and make a living when pH value is 2
It is long slow.Therefore, saccharomyces cerevisiae HKB-36 of the invention has luxuriant growth metabolism ability, and has preferable acid resistance.
Bacterial strain identification:
1. colony characteristics: being cultivated three days for 25 DEG C in malt juice liquid medium, cell spherical shape, ellipse, sausage shape, size
For (5.6-9.3) × (4.3-5.2) μm, there is precipitating to be formed.Wort agar inclined-plane culture 1 month, bacterium colony cheese shape are milky white
Color, surface is smooth, neat in edge.Corn agar Dalmau plate culture, does not generate pseudohypha, specific as shown in Figure 1.
2. bacterial strain Phylogenetic Analysis
The HKB-36 single colonie for choosing purifying is inoculated in the screening and culturing medium that pH is 5.8, in 28 DEG C of constant-temperature table cultures,
Revolving speed 120r/min；30h is cultivated, the ITS sequence of PCR amplification bacterial strain is then done using its bacterium solution as template, is sequenced, and will be surveyed
Sequence result analyzes software with Molecular Evolutionary Genetics and does its development tree, specific as shown in fig. 3, it was found that HKB-36 and registered wine brewing
The sequence homology highest of saccharomycete Saccharomyces cerevisiae, reaches 99%, it is thus determined that it is saccharomyces cerevisiae
Pseudomonas Saccharomyces cerevisiae.
3. Molecular Identification: the ITS sequence of the HKB-36 of measurement is as shown in SED ID NO:1, by it in http: //
Sequence alignment is carried out on the website archive-dtd.ncbi.nlm.nih.gov/, is analyzed based on homogeneous angular, S. cervisiae
HKB-36 is the newcomer of saccharomyces cerevisiae Pseudomonas, is named as S. cervisiae Saccharomyces cerevisiae
HKB-36。
The preparation of the fermentating metabolism product of the different S. cervisiae HKB-36 of embodiment 2
(1) preparation of seed liquor: the barms after activation are seeded to 1 ring and leach powder 145g/L, chlorine containing malt
Mycin 0.08g/L, pH value are in the fluid nutrient medium of 5.8-6.8, in 28 DEG C of -36 DEG C of constant-temperature table cultures, revolving speed 100-200r/
Min cultivates 18-30h.
(3) liquid aerobic fermentation: the seed liquor prepared is inoculated into 10%-20% inoculum concentration and contains wheat equipped with new
Bud leaches powder 145g/L, chloramphenicol 0.08g/L, and pH value is 5.8-6.8 (respectively 5.8,6.0,6.2,6.4,6.6 and 6.8)
Expanded culture in the fermentor of fluid nutrient medium, control ventilatory capacity be 1-4L/min, temperature be 28 DEG C -36 DEG C (respectively
28 DEG C, 30 DEG C, 32 DEG C, 34 DEG C and 36 DEG C), fermentation time 24-36h carries out 65 DEG C of inactivation 20min after fermentation, obtains
The fermentating metabolism product of different S. cervisiae HKB-36 without viable bacteria.
The fermentating metabolism product antibacterial experiment in vitro of 3 S. cervisiae HKB-36 of embodiment
Sterilizing Oxford cup is pressed from both sides out with aseptic nipper, it is fiery once to be first placed in rapid mistake on alcolhol burner flame, then be disposed vertically
It in the LB media surface for being inoculated with Escherichia coli, presses lightly on, makes seamless between bottom of a cup and culture medium.Each Oxford cup note
Enter 200 μ L embodiments 2 different pH and temperature under the conditions of S. cervisiae HKB-36 fermentating metabolism product, and made with physiological saline
For blank control, every kind of metabolite does 3 repetitions.For 24 hours, measurement records antibacterial circle diameter for 37 DEG C of cultures.
Specific experiment result is shown in Fig. 4.In Fig. 4, it is 5.8,6.0,6.2,6.4,6.6 and that pH 1-pH 6, which respectively indicates pH value,
It is 28 DEG C, 30 DEG C, 32 DEG C, 34 DEG C and 36 DEG C that 6.8, temperature 1- temperature 5, which respectively indicates fermentation temperature,.By response phase method to yeast
Culture fermentation parameter optimizes, it is known that when fermentation medium pH6.4,32 DEG C of fermentation temperature, the diameter of antibacterial ring size is most
Greatly, the strongest bacteriostatic effect of yeast fermentating metabolism product.
Preparation (1) slant strains of the antibacterial type biological products (yeast culture) of 4 S. cervisiae HKB-36 of embodiment
Activation: the saccharomyces cerevisiae HKB-36 of freezing is inoculated into and leaches powder 145g/L, chloramphenicol 0.08g/L and agar containing malt
On 2% slant medium, 30 DEG C of constant temperature are stood, cultivates 48h, the barms after being activated.
(2) preparation of seed liquor: the barms after activation are seeded to 1 ring and leach powder 145g/L, chlorine containing malt
Mycin 0.08g/L；PH value is in the fluid nutrient medium of 5.8-6.8, in 28 DEG C of -36 DEG C of constant-temperature table cultures, revolving speed 120-200r/
min；Cultivate 18-30h.
(3) liquid aerobic fermentation: the seed liquor prepared is inoculated into 15% inoculum concentration and is soaked equipped with new containing malt
Powder 145g/L, chloramphenicol 0.08g/L out, pH value are expanded culture in the fermentor for the fluid nutrient medium of 5.8-6.8, are controlled
Ventilatory capacity is 1-4L/min, and temperature is 28 DEG C -36 DEG C, fermentation time 24-36h.Obtain expanding the liquid spawn liquid of culture.
(4) solid anaerobic fermentation: by liquid spawn liquid using 25% inoculum concentration be seeded to mass fraction as 2-4 parts of vinasse,
In 2-4 parts of maize peel, 1-3 parts of wheat bran and 1-3 parts of apple pomace of sterile solid culture medium, addition urea control carbon-nitrogen ratio is 2-7:
1, anaerobic fermentation is carried out in controllable temperature fermentor, control moisture content is 35%-70%, and fermentation temperature is 28 DEG C -36 DEG C, hair
Ferment time 18-48h.Obtain solid fermentation product.
(5) acquisition of yeast culture product: after solid fermentation, adjusting the temperature of fermentor to 60 DEG C -75 DEG C, right
Saccharomycete in culture carries out inactivation and broken wall, it is made sufficiently to discharge mannosan, nucleic acid etc. in yeast cell contents
Substance inactivates broken wall 1h, and then culture is transferred in air-flow crushing drying machine and crush drying to get Yeast Cultivation is arrived
Object finished product.
The antibacterial experiment in vitro of the yeast culture of 5 S. cervisiae HKB-36 of embodiment
With other 5 saccharomycetes for being separated in HKB-36 and embodiment 1 of the invention for fermentation strain, root
It is prepared into according to the technique (wherein selecting fermentation medium pH 6.4,32 DEG C of fermentation temperature, fermentation time is for 24 hours) of embodiment 4
To different yeast culture products.Using Odontothrips loti carry out bacteriostatic test, observe respectively every saccharomycete to Escherichia coli,
The fungistatic effect of staphylococcus aureus and salmonella, and in addition to increasing ammonia benzyl mould using physiological saline as blank control
Element is used as positive control.
Experimental result is as shown in Figure 5.In Fig. 5, d3 indicates the resulting yeast of S. cervisiae HKB-36 fermentation of the invention
Culture, d3H, d5, d5H, d10 and d10H are respectively saccharomycete HKB-6, HKB-10, HKB-27, HKB-33 and HKB-46 difference
Ferment resulting yeast culture.Not marking below intermediate ampicillin is physiological saline blank control.
As shown in Figure 5, ampicillin all has good suppression to Escherichia coli, staphylococcus aureus and salmonella
Bacterium effect.The yeast culture that saccharomyces cerevisiae HKB-36 of the present invention is fermented also can obviously inhibit Escherichia coli, golden yellow grape
The growth of coccus and salmonella, fungistatic effect are slightly weaker than ampicillin, and the suppression according to antibacterial circle diameter, to this 3 plants of bacterium
Bacterium effect is Escherichia coli > salmonella > staphylococcus aureus.And the yeast training that the fermentation of other 5 Accharomyces cerevisiaes generates
Object is supported to Escherichia coli, staphylococcus aureus and salmonella almost without bacteriostatic activity.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim
Subject to enclosing.
SEQUENCE LISTING
<110>Beijing middle peasant Hong Ke Bioisystech Co., Ltd
<120>one Accharomyces cerevisiae bacterium HKB-36 and its application
<130> 20180705
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 554
<212> DNA
<213>unknown
<400> 1
aaaagctcaa atttgaaatc tggtaccttc ggtgcccgag ttgtaatttg gagagggcaa 60
ctttggggcc gttccttgtc tatgttcctt ggaacaggac gtcatagagg gtgagaatcc 120
cgtgtggcga ggagtgcggt tctttgtaaa gtgccttcga agagtcgagt tgtttgggaa 180
tgcagctcta agtgggtggt aaattccatc taaagctaaa tattggcgag agaccgatag 240
cgaacaagta cagtgatgga aagatgaaaa gaactttgaa aagagagtga aaaagtacgt 300
gaaattgttg aaagggaagg gcatttgatc agacatggtg ttttgtgccc tctgctcctt 360
gtgggtaggg gaatctcgca tttcactggg ccagcatcag ttttggtggc aggataaatc 420
cataggaatg tagcttgcct cggtaagtat tatagcctgt gggaatactg ccagctggga 480
ctgaggactg cgacgtaagt caaggatgct ggcataatgg ttatatgccg cccgtcttga 540
accaccggac caaa 554
Claims (8)
1. an Accharomyces cerevisiae bacterium (Saccharomyces cerevisiae) HKB-36, which is characterized in that in 2016 11
The moon is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 8th, is referred to as CGMCC, deposit number
For CGMCC No.13261.
2. a kind of fermentating metabolism object, which is characterized in that the fermentating metabolism object is S. cervisiae as described in claim 1
The fermentating metabolism product of HKB-36.
3. a kind of antibacterial biological products, which is characterized in that the antibacterial biological products include fermentation generation as claimed in claim 2
Thank to object.
4. a kind of preparation method of fermentating metabolism product as claimed in claim 2, which is characterized in that the preparation method includes
Liquid aerobic fermentation step:
Wherein, fluid nutrient medium includes that the malt of 145g/L leaches the chloramphenicol of powder and 0.08g/L, and fluid nutrient medium pH value is
5.8-6.8；
Fermentation temperature is 28 DEG C -36 DEG C, fermentation time 24-36h, ventilatory capacity 1-4L/min.
5. the preparation method of fermentating metabolism object as claimed in claim 4, which is characterized in that preparation method further includes positioned at aerobic
Seed culture step before fermentation step:
Wherein, fluid nutrient medium includes that the malt of 145g/L leaches the chloramphenicol of powder and 0.08g/L, and fluid nutrient medium pH value is
5.8-6.8；
Cultivation temperature is 28 DEG C of -36 DEG C of constant-temperature table cultures, and incubation time is 18~30h, revolving speed 120-200r/min.
6. the preparation method of fermentating metabolism object as claimed in claim 5, which is characterized in that
In aerobic fermentation step, the inoculum concentration of seed liquor is 10%~20%.
7. a kind of preparation method of antibacterial biological products as claimed in claim 3, which is characterized in that the preparation method includes
Slant strains activation, seed liquor preparation, liquid aerobic fermentation and solid anaerobic fermentation step；
Wherein, in solid anaerobic fermentation step, fermentation medium includes 2-4 parts of vinasse, 2-4 parts of maize peel, 1-3 parts of wheat bran and apple
1-3 parts of pomace, culture medium carbon-nitrogen ratio is 2-7:1, moisture content 35%-70%；
Fermentation temperature is 28 DEG C -36 DEG C, fermentation time 18-48h.
8. the preparation method of antibacterial type biological products as claimed in claim 7, which is characterized in that the preparation method further includes
After solid anaerobic fermentation the step of preparing antibacterial type biological products finished product:
Fermentation temperature is adjusted to 60 DEG C -75 DEG C, broken wall is inactivated to the S. cervisiae HKB-36, drying is then crushed, is made
Antibacterial type biological products finished product.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441343A (en) * | 2015-11-24 | 2016-03-30 | 中国农业科学院饲料研究所 | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof |
| WO2018047123A1 (en) * | 2016-09-12 | 2018-03-15 | The New Zealand Institute For Plant And Food Research Limited | Biological control of plant pathogenic microorganisms |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
-
2018
- 2018-09-25 CN CN201811116994.8A patent/CN109401990B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441343A (en) * | 2015-11-24 | 2016-03-30 | 中国农业科学院饲料研究所 | Saccharomyces cerevisiae and Saccharomyces cerevisiae culture thereof |
| WO2018047123A1 (en) * | 2016-09-12 | 2018-03-15 | The New Zealand Institute For Plant And Food Research Limited | Biological control of plant pathogenic microorganisms |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
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