CN103859149A - Production technology of lactobacillus fermented soybean meal - Google Patents
Production technology of lactobacillus fermented soybean meal Download PDFInfo
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- CN103859149A CN103859149A CN201210550579.XA CN201210550579A CN103859149A CN 103859149 A CN103859149 A CN 103859149A CN 201210550579 A CN201210550579 A CN 201210550579A CN 103859149 A CN103859149 A CN 103859149A
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- lactic acid
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Abstract
The invention discloses a production technology of lactobacillus fermented soybean meal. The production technology comprises the following steps: (1) lactobacillus strain activation; (2) lactobacillus strain liquid culture; (3) lactobacillus strain culture in a serum bottle; and (4) soybean meal fermentation. The production technology is simple and easy to understand; lactobacillus can promote animal growth, adjust gastrointestinal normal floras, and maintain microecological balance so as to improve gastrointestinal functions, a lactobacillus micro ecological preparation provides an efficient, harmless, non-polluting new choice for feed and animal husbandry and aquaculture industries, and by using of characteristics of the animal floras themselves and the relationship between soil and environment, the lactobacillus micro ecological preparation is used scientifically and rationally, so that largest ecological effects and economic benefits can be obtained.
Description
Technical field
The invention belongs to the production technology of dregs of beans, be specifically related to a kind of production technology of lactobacillus-fermented dregs of beans.
Background technology
Dregs of beans is one of important feedstuff of livestock rearing industry and culture fishery, accounts for 80%～90% of protein feed.Dregs of beans is the byproduct that soybean obtains after extracting bean oil, it is a kind of plant protein fodder of excellence, and be protein raw materials main in animal feed, there is crude protein content high, amino acid composition rationally, animal digestion utilization rate advantages of higher, and containing what enrich, can improve the isoflavones of animal immunizing power, but in dregs of beans, exist some to affect the ANFs of animal Nutrients Digestion and physiological health, comprise antigen protein, phytic acid, soyabean oligosaccharides, saponin, trypsin ihhibitor, urase etc., nutritive value and the utilization rate of feed are not only reduced, and reduce the production performance of livestock and poultry.The soya-bean polypeptides producing after soybean protein hydrolysis is made up of 2～9 amino acid conventionally, soya-bean polypeptides has unique processing characteristics than soybean protein, large quantity research shows, absorption aligned protein and the amino acid whose absorption of animal to little peptide is more quick, and absorbing of little peptide can promote the absorption of animal to other nutrition, thereby significantly reduce the generation of Animal diseases, therefore soya-bean polypeptides has wide exploitation prospect.
At present, biofermentation technique is the effective ways that improve dregs of beans quality.Antigen protein and high molecular weight protein are resolved into little peptide and the various bioactivators that can be digested and assimilated by animal by the protease producing during the fermentation, and soybean protein can be transformed into mycoprotein by microorganism simultaneously, has improved the quality of protein.The fragranced, the sauce fragrance that produce during the fermentation have obvious food calling effect, have improved the palatability of feed.But fermented bean dregs market is more random at present, and market product quality is uneven, and index is evaluated without unified standard.
Summary of the invention
The problems referred to above that exist in order to overcome prior art field, the object of the invention is to, a kind of production technology of lactobacillus-fermented dregs of beans is provided, solve current fermented bean dregs market more random, market product quality is uneven, and index is evaluated the problem without unified standard.
The production technology of lactobacillus-fermented dregs of beans provided by the invention, it comprises following concrete steps:
(1) lactic acid bacteria culturers activation, A, making lactic acid bacterium seed culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, agar 15g, distilled water 1000mL making lactic acid bacterium seed culture medium, autoclaving 15min at 121 DEG C; B, activation lactic acid bacteria culturers: lactic acid bacteria culturers is transferred on the lactic acid bacteria seed culture medium having prepared and is activated, cultivate 48 hours for 37 DEG C;
(2) lactic acid bacteria culturers Liquid Culture, A, making lactic acid bacteria liquid culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 30 minutes at 121 DEG C; B, cultivation lactic acid bacteria culturers liquid: picking activated spawn inoculation fluid nutrient medium 5ml, cultivates 48 hours for 37 DEG C;
(3) lactic acid bacteria culturers serum bottle is cultivated, A, lactic acid bacteria culturers Liquid Culture: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 15min at 121 DEG C; B, lactic acid bacteria culturers serum bottle are cultivated: picking activation lactic acid bacteria culturers inoculating lactic acid bacterium bacterial classification fluid nutrient medium 50ml, cultivates 24 hours, is extended to successively 5L for 37 DEG C;
(4) bean pulp fermentation, A, dregs of beans pretreatment: after dregs of beans is pulverized, cross 40 mesh sieves; B, preparation dregs of beans liquid fermentation medium: press dregs of beans 10g, wheat bran 2g, water 100ml, natural pH value is prepared, 121 DEG C of sterilizings 20 minutes; C, bean pulp fermentation: lactic acid bacteria is added in the dregs of beans liquid fermentation medium of sterilizing with the inoculum concentration of 5% (v/w), mix, ferment, 37 DEG C ferment 72 hours.
The production technology of lactobacillus-fermented dregs of beans provided by the invention, its beneficial effect is, this technique is easily understood, it is high that the dregs of beans of producing has crude protein content, amino acid composition rationally, animal digestion utilization rate advantages of higher, and containing what enrich, can improve the isoflavones of animal immunizing power, lactic acid bacteria can promote growth of animal, regulate composition of gut flora, maintain microecological balance, thereby improve gastrointestinal function, Strains of lactobacillus for feed and animal husbandry and fishery aquaculture provide one efficient, harmless, free of contamination new selection, utilize the relation between animal flora own characteristic and soil and environment, use scientifically and rationally, make it reach maximum ecological effect and economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, to the production technology of lactobacillus-fermented dregs of beans provided by the invention, be described in detail.
Embodiment
With reference to the production technology of the lactobacillus-fermented dregs of beans of the present embodiment, it comprises following concrete steps:
(1) lactic acid bacteria culturers activation, A, making lactic acid bacterium seed culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, agar 15g, distilled water 1000mL making lactic acid bacterium seed culture medium, autoclaving 15min at 121 DEG C; B, activation lactic acid bacteria culturers: lactic acid bacteria culturers is transferred on the lactic acid bacteria seed culture medium having prepared and is activated, cultivate 48 hours for 37 DEG C;
(2) lactic acid bacteria culturers Liquid Culture, A, making lactic acid bacteria liquid culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 30 minutes at 121 DEG C; B, cultivation lactic acid bacteria culturers liquid: picking activated spawn inoculation fluid nutrient medium 5ml, cultivates 48 hours for 37 DEG C;
(3) lactic acid bacteria culturers serum bottle is cultivated, A, lactic acid bacteria culturers Liquid Culture: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 15min at 121 DEG C; B, lactic acid bacteria culturers serum bottle are cultivated: picking activation lactic acid bacteria culturers inoculating lactic acid bacterium bacterial classification fluid nutrient medium 50ml, cultivates 24 hours, is extended to successively 5L for 37 DEG C;
(4) bean pulp fermentation, A, dregs of beans pretreatment: after dregs of beans is pulverized, cross 40 mesh sieves; B, preparation dregs of beans liquid fermentation medium: press dregs of beans 10g, wheat bran 2g, water 100ml, natural pH value is prepared, 121 DEG C of sterilizings 20 minutes; C, bean pulp fermentation: lactic acid bacteria is added in the dregs of beans liquid fermentation medium of sterilizing with the inoculum concentration of 5% (v/w), mix, ferment, 37 DEG C ferment 72 hours.
The production technology of lactobacillus-fermented dregs of beans provided by the invention, adopts lactic acid bacteria class preparation, for feed and animal husbandry and fishery aquaculture provide efficient, harmless, a free of contamination new selection.Along with increasing novel bacterial and specific strains are used aborning, we to take into full account animal flora own characteristic and post soil and environment between relation, scientifically and rationally use, make it reach maximum ecological effect and economic benefit.
Claims (1)
1. a production technology for lactobacillus-fermented dregs of beans, is characterized in that: it comprises following concrete steps:
(1) lactic acid bacteria culturers activation, A, making lactic acid bacterium seed culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, agar 15g, distilled water 1000mL making lactic acid bacterium seed culture medium, autoclaving 15min at 121 DEG C; B, activation lactic acid bacteria culturers: lactic acid bacteria culturers is transferred on the lactic acid bacteria seed culture medium having prepared and is activated, cultivate 48 hours for 37 DEG C;
(2) lactic acid bacteria culturers Liquid Culture, A, making lactic acid bacteria liquid culture medium: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 30 minutes at 121 DEG C; B, cultivation lactic acid bacteria culturers liquid: picking activated spawn inoculation fluid nutrient medium 5ml, cultivates 48 hours for 37 DEG C;
(3) lactic acid bacteria culturers serum bottle is cultivated, A, lactic acid bacteria culturers Liquid Culture: soak powder 5g, beef peptone 10g, glucose 20g, tween (80) 1ml by tryptone 10g, yeast, dipotassium hydrogen phosphate 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 200mg, manganese sulfate 50mg, distilled water 1000mL preparation, autoclaving 15min at 121 DEG C; B, lactic acid bacteria culturers serum bottle are cultivated: picking activation lactic acid bacteria culturers inoculating lactic acid bacterium bacterial classification fluid nutrient medium 50ml, cultivates 24 hours, is extended to successively 5L for 37 DEG C;
(4) bean pulp fermentation, A, dregs of beans pretreatment: after dregs of beans is pulverized, cross 40 mesh sieves; B, preparation dregs of beans liquid fermentation medium: press dregs of beans 10g, wheat bran 2g, water 100ml, natural pH value is prepared, 121 DEG C of sterilizings 20 minutes; C, bean pulp fermentation: lactic acid bacteria is added in the dregs of beans liquid fermentation medium of sterilizing with the inoculum concentration of 5% (v/w), mix, ferment, 37 DEG C ferment 72 hours.
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| CN201210550579.XA CN103859149A (en) | 2012-12-18 | 2012-12-18 | Production technology of lactobacillus fermented soybean meal |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886423A (en) * | 2016-01-20 | 2016-08-24 | 江苏盐城源耀生物科技有限公司 | Preparation method of biofermentation rice bran meal |
| CN109527197A (en) * | 2018-11-20 | 2019-03-29 | 湖北希普生物科技有限公司 | The method for preparing high acidic fermentation dregs of beans using bacillus coagulans |
| CN110024904A (en) * | 2019-04-29 | 2019-07-19 | 湖南粒丰生物科技有限公司 | A kind of preparation method of aquatic products lactobacillus-fermented soya-bean milk |
| CN110651898A (en) * | 2019-11-11 | 2020-01-07 | 白城市隆盛实业科技有限公司 | Fermentation method of soybean meal |
| CN115251241A (en) * | 2022-07-12 | 2022-11-01 | 廉江双胞胎饲料有限公司 | Continuous production method of fermented soybean meal |
-
2012
- 2012-12-18 CN CN201210550579.XA patent/CN103859149A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886423A (en) * | 2016-01-20 | 2016-08-24 | 江苏盐城源耀生物科技有限公司 | Preparation method of biofermentation rice bran meal |
| CN109527197A (en) * | 2018-11-20 | 2019-03-29 | 湖北希普生物科技有限公司 | The method for preparing high acidic fermentation dregs of beans using bacillus coagulans |
| CN110024904A (en) * | 2019-04-29 | 2019-07-19 | 湖南粒丰生物科技有限公司 | A kind of preparation method of aquatic products lactobacillus-fermented soya-bean milk |
| CN110651898A (en) * | 2019-11-11 | 2020-01-07 | 白城市隆盛实业科技有限公司 | Fermentation method of soybean meal |
| CN115251241A (en) * | 2022-07-12 | 2022-11-01 | 廉江双胞胎饲料有限公司 | Continuous production method of fermented soybean meal |
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Application publication date: 20140618 |