CN106699412A - Method for cultivating stropharia rugosoannulata farlow - Google Patents
Method for cultivating stropharia rugosoannulata farlow Download PDFInfo
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- CN106699412A CN106699412A CN201710072958.5A CN201710072958A CN106699412A CN 106699412 A CN106699412 A CN 106699412A CN 201710072958 A CN201710072958 A CN 201710072958A CN 106699412 A CN106699412 A CN 106699412A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for cultivating stropharia rugosoannulata farlow. The method comprises a mother strain preparation method and a breeder seed preparation method. The method sequentially comprises stirring, bagging, sterilizing, inoculating and spawn running; a formula of the mother strain preparation method is prepared from 200g of peeled potato, 100g of corn flour, 20g of agar, 20g of glucose, 0.3g of oxygenate, and 1000ml of water; a formula of the mother strain preparation method is prepared from 200g of peeled potato, 100g of corn flour, 20g of agar, 20g of glucose, 2g of monopotassium phosphate, 1g of magnesium sulfate, 8g of peptone, 10mg of vitamin B1, 0.3g of oxygenate, and 100ml of water. Therefore, the method provided by the invention has the advantages of simple technique, fast fungus growth speed, and successful planting.
Description
Technical field
A kind of technical field the invention belongs to cultivate mushroom, and in particular to method of cultivation Stropharia rugoso-annulata.
Background technology
Mushroom has many benefits for the healthy of people, and mushroom is rich in more protein, carbohydrate and dimension
Raw element etc., also has trace element and mineral matter in addition, and eating mushroom can strengthen body immunity more, therefore, for the cultivation of mushroom
It is that very important, different breeding method can cultivate some new mushrooms, there is also some bacterium for Stropharia rugoso-annulata at present
The breeding method of class, but there is more or less weak point in these methods, and such as speed of production is slow, and technical sophistication is bad
A kind of the shortcomings of plantation etc., for these weak points, it is necessary to plantation of developing that technology is simple, fungus grown speed is become reconciled soon
Cultivation Stropharia rugoso-annulata method；Therefore, the big spherical cap of cultivation that a kind of technology is simple, fungus grown speed becomes reconciled plantation soon is developed
The method tool of mushroom is of great significance.
The content of the invention
The invention aims to overcome the deficiencies in the prior art, and provide that a kind of technology is simple, fungus grown speed
Become reconciled soon plantation cultivation Stropharia rugoso-annulata method.
The object of the present invention is achieved like this：A kind of method for cultivating Stropharia rugoso-annulata, methods described is divided into parent species making
Method and original seed preparation method, methods described are followed successively by stirring, pack, sterilizing, inoculation and hair bacterium.
Formula in the parent species preparation method is 200 grams of peeling potatoes, 100 grams of corn flour, 20 grams of agar, glucose
20 grams, 0.3 gram of oxygenation agent, 1000 milliliters of water.
Formula in the parent species preparation method is 200 grams of peeling potatoes, 100 grams of corn flour, 20 grams of agar, glucose
20 grams, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, (10 milligrams of vitamin B1 of 8 grams of peptone）, 0.3 gram of oxygenation agent, the milli of water 1000
Rise.
Formula in the original seed preparation method is 35 jin of rice husk, 35 jin of corncob, 20 jin of wheat bran, 10 jin of corn flour, stone
2 jin of ash, 1 jin of white sugar, 2 jin of gypsum, 0.5 jin of special mycelia augmentin, 0.3 jin of anti-fouling agent, 130 jin of water.
Formula in the original seed preparation method is 30 jin of rice husk, 20 jin of corncob, wooden Xiao（Broad leaf tree）20 jin, wheat bran 20
Jin, 10 jin of corn flour, 1 jin of white sugar, 2 jin of lime, 2 jin of gypsum, 0.5 jin of special mycelia augmentin, 0.3 jin of anti-fouling agent, water 130
Jin.
Formula in the original seed preparation method is wheat（Grain）100 jin, 1 jin of lime, 2 jin of gypsum, special mycelia increases
0.5 jin of element long, 0.3 jin of anti-fouling agent.
Beneficial effects of the present invention：Preparation method uses stirring, pack, sterilizing, inoculation and sends out bacterium in the present invention, by stirring
Mix so that nutrition liquid energy stirs, the purpose of pack is that, in order to preferably be inoculated with, sterilizing is the favourable life for Stropharia rugoso-annulata
Long, inoculation is in order to the hair bacterium of next step is prepared；The preparation method technology is simple, good plantation, can be adapted to Stropharia rugoso-annulata bacterium
Plant fast-growth；Therefore, the present invention have the advantages that technology simply, fungus grown speed becomes reconciled plantation soon.
Specific embodiment
A kind of method for cultivating Stropharia rugoso-annulata, methods described is divided into parent species preparation method and original seed preparation method, the side
Method is followed successively by stirring, pack, sterilizing, inoculation and hair bacterium；Formula in the parent species preparation method is 200 grams of peeling potatoes,
100 grams of corn flour, 20 grams of agar, 20 grams of glucose, 0.3 gram of oxygenation agent, 1000 milliliters of water；Matching somebody with somebody in the parent species preparation method
It is just 200 grams of peeling potatoes, 100 grams of corn flour, 20 grams of agar, 20 grams of glucose, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate,
(10 milligrams of vitamin B1 of 8 grams of peptone）, 0.3 gram of oxygenation agent, 1000 milliliters of water.
With the formula in parent species preparation method as 200 grams of peeling potatoes in the present invention, 100 grams of corn flour, 20 grams of agar,
20 grams of glucose, 0.3 gram of oxygenation agent as a example by 1000 milliliters of water, illustrates preparation method：First have to prepare a bite Sheng water
Vat, weighs corresponding water and siccative is poured in cylinder（It suppose 100 jin of mixed dry materials, 115 jin of water）, then other auxiliary materials are dissolved in
In water, lime clarified solution, slightly point lime face is also not serious, and preferably nutrient solution is stirred evenly（The nutrient solution totality for having configured
The total Water of requirement is not exceeded）.Major ingredient（Rice husk, maize flour, wheat bran）Weigh up on request on a clean ash-pan
First dry mixing is uniform, and nutrient solution is splashed on compound, the stirring when splashing, and stops to turn again a little while after having turned.It is interrupted and turns over
Mix and repeatedly material is mixed thoroughly, there must not be siccative, in case sterilizing is not thorough；Its cold mixing material is preferably overnight to refill pouch；Dress pouch：
It to be 12 centimeters from flat footpath, the special cultivating bag of strain long 24 centimeters is tied one of pouch in advance（Knot that can be undone by a pull is pricked to be easy to
Untie）, pricking head will reserve 1.5 centimeters long, be easy to inoculation；Can also use 15 centimeters wide, 30 centimeters of strain bags long, during pack,
Want write by hand one that wet feed is put into bag, point three layers of side rim hand pressure are real untill filling（Reserve 1.5 centimeters of bundles long
Head）, elastic moderate, material is installed；Sterilizing：The pouch for installing is put into the sterilizing pond built up in advance（Bottom of pond will spread wood
Plate）, finally covered with bilayer film and with sandbag compress can igniting sterilize, to be given much attention to during sterilizing water level in pot number
（Water has lacked and has just added water）, prevent from dryouting pot.When temperature reaches 100 DEG C in pond, material cloth will be heaved, and be counted from now（Material cloth begins
To heave eventually and to have point pressure）Maintenance can cease fire in 10 hour；Pouch overnight will wait the next morning to take the dish out of the pot again in pond
（This point is especially important）Inoculation：Pouch goes out Chi Houyao and is placed on the cool frame of preprepared to cool, when bag temperature is below 30 DEG C
When can just be inoculated with.Pouch is put into one of inoculating hood during inoculation sets and piled entirely until one, then tweezers, alcohol swab
Ball, strain etc. are put in, and the sterilizing of aerosol disinfection box, sterilizing half are can use after box opening ultrathin film is obturaged again then
Can be inoculated with after individual hour；（Specific detailed operational procedure about being inoculated with just seldom is said herein）All pouches in case
Ability handle has been connect completely to take out；Then the pouch being inoculated with is put into hair bacterium room again and sends out bacterium just；All pockets are completed like this
；Hair bacterium：All being put into by after all material have been mixed, install, gone out and connect in preprepared hair bacterium room is carried out
Hair bacterium；Cultivating greenhouse without hair bacterium room can also first when hair bacterium canopy is used, and with canopy is cultivated when bacterium canopy is sent out, Kao Pengqiang is desirable to build
Individual 0.4 meter of fungus shelf high from the ground, fungus shelf bamboo pole is built smooth, can not cross gap；0.9 meter wide can put four row's pouches,
Often row pouch can put 8 it is high.So be conducive to divulging information and sending out bacterium；Hair bacterium canopy is dark, and room temperature is preferably kept in 16 DEG C to 28 DEG C
Between, the bacterium for so sending out, mycelia is dense sturdy.More can not high temperature suddenly, in order to avoid mycelia is aggrieved or pocket souring is fouled；In canopy also
Intermittent air blast is wanted, keeps air fresh.Pouch will turn over once bag every seven days or so（Often turn over bag and be conducive to overall pouch mycelia
Grow）, to upset original position when turning over bag, the place that the good pouch of hair bacterium is placed on base or cooled, sending out
The bad pouch of bacterium is placed on outer row or top；And choose the pouch for having miscellaneous bacteria（Part have miscellaneous bacteria can also utilize be placed on one
Block sends out bacterium, and immediately clearing out for miscellaneous bacteria especially severe is buried outside canopy；Miscellaneous bacteria quantity is conditional when more can also broken bag again
Break pack disinfection inoculation into pieces with one's fingers）Go down like this, general 25 or so can all send out full bacterium；A thermometer is preferably put to see in hair bacterium room
Room temperature is examined, extremes of temperature is prevented；Will take measures pre- rat preservative in advance simultaneously, to reduce unnecessary loss；Therefore, originally
Invention have the advantages that technology simply, fungus grown speed becomes reconciled plantation soon.
Specific embodiment is unrestricted to of the invention further illustrating, and is existed for those of ordinary skills
Further conversion is done in the case of not departing from substance of the present invention to structure, and all these conversion should all belong to institute of the present invention
Attached scope of the claims.
Claims (6)
1. it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：Methods described is divided into parent species preparation method and original seed making side
Method, methods described is followed successively by stirring, pack, sterilizing, inoculation and hair bacterium.
2. it is according to claim 1 it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：In the parent species preparation method
Formula be 200 grams of peeling potatoes, 100 grams of corn flour, 20 grams of agar, 20 grams of glucose, 0.3 gram of oxygenation agent, the milli of water 1000
Rise.
3. it is according to claim 1 it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：In the parent species preparation method
Formula be 200 grams of peeling potatoes, 100 grams of corn flour, 20 grams of agar, 20 grams of glucose, 2 grams of potassium dihydrogen phosphate, magnesium sulfate
1 gram, (10 milligrams of vitamin B1 of 8 grams of peptone）, 0.3 gram of oxygenation agent, 1000 milliliters of water.
4. it is according to claim 1 it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：In the original seed preparation method
Formula be 35 jin of rice husk, 35 jin of corncob, 20 jin of wheat bran, 10 jin of corn flour, 2 jin of lime, 1 jin of white sugar, 2 jin of gypsum is special
0.5 jin of mycelia augmentin, 0.3 jin of anti-fouling agent, 130 jin of water.
5. it is according to claim 4 it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：In the original seed preparation method
Formula be 30 jin of rice husk, 20 jin of corncob, wooden Xiao（Broad leaf tree）20 jin, 20 jin of wheat bran, 10 jin of corn flour, 1 jin of white sugar, lime
2 jin, 2 jin of gypsum, 0.5 jin of special mycelia augmentin, 0.3 jin of anti-fouling agent, 130 jin of water.
6. it is according to claim 1 it is a kind of cultivate Stropharia rugoso-annulata method, it is characterised in that：In the original seed preparation method
Formula be wheat（Grain）100 jin, 1 jin of lime, 2 jin of gypsum, 0.5 jin of special mycelia augmentin, 0.3 jin of anti-fouling agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072958.5A CN106699412A (en) | 2017-02-10 | 2017-02-10 | Method for cultivating stropharia rugosoannulata farlow |
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| CN201710072958.5A CN106699412A (en) | 2017-02-10 | 2017-02-10 | Method for cultivating stropharia rugosoannulata farlow |
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| CN201710072958.5A Pending CN106699412A (en) | 2017-02-10 | 2017-02-10 | Method for cultivating stropharia rugosoannulata farlow |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107500847A (en) * | 2017-10-20 | 2017-12-22 | 伊犁师范学院 | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof |
| CN114621876A (en) * | 2021-04-30 | 2022-06-14 | 宝丰县弘亚食用菌科技有限公司 | Preparation method of stropharia rugoso-annulata strain |
| CN116034813A (en) * | 2022-12-13 | 2023-05-02 | 新疆农业科学院植物保护研究所 | Stropharia rugoso-annulata generation medium and fungus bag preparation method |
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| CN1817089A (en) * | 2006-01-05 | 2006-08-16 | 李永林 | Culturing materials of macrospheric mushroom |
| CN101723758A (en) * | 2009-10-30 | 2010-06-09 | 山东芳绿农业科技有限公司 | Method for culturing stropharia rugoso-annulata and compost thereof |
| CN101946636A (en) * | 2010-09-16 | 2011-01-19 | 四川省农业科学院土壤肥料研究所 | Quick production method for large ball-cover mushroom strains |
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Patent Citations (3)
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| CN1817089A (en) * | 2006-01-05 | 2006-08-16 | 李永林 | Culturing materials of macrospheric mushroom |
| CN101723758A (en) * | 2009-10-30 | 2010-06-09 | 山东芳绿农业科技有限公司 | Method for culturing stropharia rugoso-annulata and compost thereof |
| CN101946636A (en) * | 2010-09-16 | 2011-01-19 | 四川省农业科学院土壤肥料研究所 | Quick production method for large ball-cover mushroom strains |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107500847A (en) * | 2017-10-20 | 2017-12-22 | 伊犁师范学院 | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof |
| CN114621876A (en) * | 2021-04-30 | 2022-06-14 | 宝丰县弘亚食用菌科技有限公司 | Preparation method of stropharia rugoso-annulata strain |
| CN116034813A (en) * | 2022-12-13 | 2023-05-02 | 新疆农业科学院植物保护研究所 | Stropharia rugoso-annulata generation medium and fungus bag preparation method |
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Application publication date: 20170524 |