CN107058134A - A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used - Google Patents
A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used Download PDFInfo
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- CN107058134A CN107058134A CN201710360091.3A CN201710360091A CN107058134A CN 107058134 A CN107058134 A CN 107058134A CN 201710360091 A CN201710360091 A CN 201710360091A CN 107058134 A CN107058134 A CN 107058134A
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Abstract
The present invention relates to a kind of breeding method of Cordyceps militaris strain and application and its culture medium used, this method includes the pure culture of Cordyceps militaris being inoculated into fluid nutrient medium, and shaking table culture obtains liquid spawn；The fluid nutrient medium in parts by weight, including：9 10 parts of potato, the leachate of fresh 9 10 parts of silkworm chrysalis, 0.8 1 parts of glucose, 0.8 1 parts of peptone, 0.8 1 parts of yeast extract, 0.09 0.1 parts of potassium dihydrogen phosphate, 0.04 0.05 parts of magnesium sulfate, 0.09 0.1 parts of degreased pupa powder, 0.0005 0.0006 parts of sodium hydroxide, 45 60 parts of water, 0.02 part of vitaminB10 .01；The parts by weight of the water include the water in silkworm chrysalis leachate.The present invention is by the special formulation of liquid spawn and combines shaking table culture, the hereditary capacity of strain can be well kept, strain is reduced and the degeneration occurred in fracture is decomposed in mycelia, keep spawn activity and bacterium germination speed, promote follow-up mycelial annesl, increase fruiting body yield.
Description
Technical field
The present invention relates to the technical field of Applied Biotechnology principle artificial culture fungi, and in particular to the training of Cordyceps militaris
Educate, relate more specifically to the breeding method and application and its culture medium that uses of a kind of Cordyceps militaris strain.
Background technology
Cordyceps militaris（Cordyceps militaris）It is under the jurisdiction of mycota（Fungi）, Ascomycota（Ascomycota）、
Ascomycetes（Ascomycetes）, excrement shell bacterium subclass（Sordariomycetidae）, Hypocreales（Hypocreales）, wheat
Angle Cordycepps（Clavicipitaceae）, Cordyceps（Cordyceps）.Cordyceps militaris is that the ascus of very famous medicine-food two-purpose is true
Bacterium, is also the main source for obtaining cordycepin, with very high value of exploiting and utilizing.In recent years, as wild cordyceps are provided
The increasingly exhaustion in source, its price constantly rises, so as to promote the substitute of people's searching wild cordyceps.Due to Cordyceps militaris tool
There is the medicinal and edibility close with cordyceps sinensis, Cordyceps militaris is more prone to domestication in addition, therefore more and more by people
Concern.
But, due to the not comprehensive nutrition of liquid formulations in traditional handicraft, cause Cordyceps militaris bacterium germination slow, mycelia is easier to always
Change, growing way is weak, and mycelium color and luster is dark, and fruiting body yield is low.Therefore, our culture formula of liquid, liquid bacterias to liquid spawn
The breeding method planted is improved.
The content of the invention
The invention provides a kind of breeding method of Cordyceps militaris strain, this method includes：
The pure culture of Cordyceps militaris is inoculated into fluid nutrient medium, shaking table culture obtains liquid spawn；
The fluid nutrient medium in parts by weight, including：9-10 parts of potato, fresh silkworm chrysalis 9-10 parts of leachate, grape
It is sugared 0.8-1 parts, 0.8-1 parts of peptone, 0.8-1 parts of yeast extract, 0.09-0.1 parts of potassium dihydrogen phosphate, magnesium sulfate 0.04-0.05
Part, 0.09-0.1 parts of degreased pupa powder, 0.0005-0.0006 parts of sodium hydroxide, 45-60 parts of water, vitamin B1 0.01-0.02
Part；
The parts by weight of the water include the water in silkworm chrysalis leachate.
The present invention is by the special formulation of liquid spawn and combines shaking table culture, can well keep the hereditary capacity of strain,
Reduce strain and the degeneration occurred in fracture is decomposed in mycelia, keep spawn activity and bacterium germination speed, promote follow-up mycelial turn
Color, increases fruiting body yield.Solve the problems such as Cordyceps militaris spawn is degenerated, bacterium germination is weak, later stage annesl is slow, fruiting body yield is few.
Further, the fluid nutrient medium in parts by weight, including：9.5 parts of potato, the leaching of fresh 9.5 parts of silkworm chrysalis
Go out liquid, 0.95 part of glucose, 0.95 part of peptone, 0.95 part of yeast extract, 0.095 part of potassium dihydrogen phosphate, magnesium sulfate 0.048
Part, 0.095 part of degreased pupa powder, 0.00057 part of sodium hydroxide, 47 parts of water, 0.014 part of vitamin B1；The parts by weight of the water
Number includes the water in silkworm chrysalis leachate.
Further, the condition of the shaking table culture is：With 200-250r/min shaking table cultures 5-6 days, cultivation temperature was 20-
22℃。
Further, the silkworm chrysalis leachate is that fresh silkworm chrysalis is cooked to the silkworm chrysalis filtered fluid obtained after 25-35min.
Further, the protein content of the potato is not less than 3%, and content of starch is not less than 13%, preferably 13%-15%.
Further, the total nitrogen content of the peptone is not less than 13.5%, and amino acid content is not less than 3%.
Further, the protein content of the degreased pupa powder is 60%-80%, and fat content is 5%-8%.
The breeding method of Cordyceps militaris strain of the present invention can be widely used in the cultivation of Cordyceps militaris, so as to improve Cordyceps militaris
The production efficiency and yield of fructification.
The present invention also provides a kind of method for cultivating Cordyceps militaris, including：By the breeding method of above-mentioned Cordyceps militaris strain
The liquid spawn of preparation is forwarded to fructification culture medium, proceeds culture.
Further, the used silkworm chrysalis of silkworm chrysalis leachate is put into fructification culture medium.
The present invention provides a kind of nutrient solution for cultivating Cordyceps militaris strain, in parts by weight, including：Potato 9-
10 parts, fresh silkworm chrysalis 9-10 parts of leachate, 0.8-1 parts of glucose, 0.8-1 parts of peptone, 0.8-1 parts of yeast extract, di(2-ethylhexyl)phosphate
0.09-0.1 parts of hydrogen potassium, 0.04-0.05 parts of magnesium sulfate, 0.09-0.1 parts of degreased pupa powder, sodium hydroxide 0.0005-0.0006
Part, 45-60 parts of water, 0.01-0.02 parts of vitamin B1；The parts by weight of the water include the water in silkworm chrysalis leachate.
The above-mentioned nutrient solution of the present invention can be used for the cultivation of Cordyceps militaris strain, makes the strain of acquisition be difficult to degenerate, protects
Spawn activity and bacterium germination speed are held, promotes follow-up mycelial annesl, increases fruiting body yield.
Embodiment
In the liquid spawn breeding method of Cordyceps militaris, moving back for the strain that culture making is obtained is carried out according to different formulas
Change degree is different with resistance.Solid test tube kind is transferred in liquid shaking table culture liquid by it, passes through culture for some time
Shaking table liquid spawn is obtained, then liquid spawn is transferred in fructification culture medium again, continues to cultivate and sees light for a period of time, carry out
Fructification culture is that can obtain that pollution rate is low, high yield cordyceps militaris sporocarp.Pass through the Cordyceps militaris spawn of different nutrient solution cultures
Growth characteristics and fruiting body yield have very big difference, by the present invention culture formula of liquid make liquid spawn cause strain bacterium
Filament is more, and strain properties are stable, efficiently can breed and grow.
Cordyceps militaris breeding strain is carried out The embodiment provides a kind of nutrient solution of use special formulation
Method, including fluid nutrient medium is prepared, then solid test tube strains are transferred in liquid spawn in an aseptic environment, then by liquid
Body strain is placed to be shaken 5-6 days processed on shaking table with 250r/min, and lucifuge cultivation temperature is 20-22 DEG C, then will shake the liquid bacteria made
Plant and be transferred on fructification culture medium.
The present invention is further detailed explanation by the following examples, but these contents should not be construed as to the present invention
Limitation.The operating method not stated clearly specifically wherein is carried out according to the routine operation of this area, reagent, raw material and the strain used
Obtained Deng commercially available.Wherein the component of culture medium unless otherwise instructed, then purchased from the extensive and profound in meaning star in Beijing.
Embodiment
Fluid nutrient medium is prepared first, is specifically included：Potato 200g is weighed, boils and residue is filtered after 30min, Ma Ling is obtained
Potato leachate.Silkworm chrysalis 200g living is put into addition 1200ml or so water in pot to be cooked, silkworm is filtrated to get after about 30 minutes
Pupa leachate.It is 1000ml and admixed together to control potato leachate and the total amount of liquid of silkworm chrysalis leachate, is then distinguished
Add glucose 20g, peptone 20g, yeast leaching 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, degreased pupa powder 2g, sodium hydroxide
0.012g, B1 0.3g, dissolving stir and are divided in 500L triangular flasks, and every bottle of about dress takes nutrient solution 200L, during sealing
Position of bottleneck puts one piece of 2cm*2cm sterile cotton, to increase oxygen for later stage liquid spawn.
The triangular flask that will be equipped with liquid is positioned in autoclave, and steam sterilizing is carried out under 0.12-0.18MPa, and temperature is
118-121 DEG C is sterilized 30 minutes, and pot is opened when pressure drops to normal pressure and takes out the triangular flask nutrient solution sterilized, then takes out and puts
It is cool standby.
The triangular flask sterilized is put into superclean bench, sterilize 30-40min under ultraviolet lamp, then ultra-clean
Solid test tube kind is transferred in the triangular flask gone out in workbench, then triangular flask is put into the dark training room sterilized, is being shaken
Shaken and made 6 days with 250r/min on bed, lucifuge cultivation temperature is 21 DEG C, you can obtain shaking the liquid spawn made.
Liquid spawn is connected in fructification culture medium, bacterium germination annesl and fructification culture is carried out, obtains within 40-55 days sub real
Body.
In the present embodiment, dried silkworm chrysalis meal and silkworm chrysalis leachate are with the addition of in fluid nutrient medium can enable the heredity of strain
Good holding, reduces strain and the degeneration occurred in fracture is decomposed in mycelia, strain is kept the state of most original.Silkworm chrysalis is leached
Used silkworm chrysalis can be put into fructification culture medium in liquid, inoculated liquid spawn and carried out fructification culture, can so keep away
Exempt to waste.The fructification of the more simple wheat of selection color and luster, condition or rice is good, and yield is also relatively high.
The raw material of each culture medium passes through strict screening, and such as dried silkworm chrysalis meal, peptone, living silkworm chrysalises and potato require matter
Amount reaches a standard, and is regular manufacturer.Wherein, the buying of potato is relative wants stricter：It is required that nothing is rotted, nothing is gone mouldy, nothing
Germination, not damaged, the fresh potato without green change, intact wound, its protein content is 5%, and content of starch is 25%.Silkworm chrysalis
Choose this season living silkworm chrysalises：It is fresh, uniform in size, without wound, free from extraneous odour, carried out cleaning and sterilizing using preceding.The total nitrogen of peptone
Content is 18.5%, and amino acid content is 4.5%.The protein content of degreased pupa powder is 75%, and fat content is 7%.
Comparative example
The step of according to embodiment, cultivates Cordyceps militaris, except that using the fluid nutrient medium of consisting of：20% potato,
2% glucose, 0.2% peptone, 0.09% potassium dihydrogen phosphate, 0.04% magnesium sulfate, surplus is water, wherein the processing of potato and reality
Apply example identical.Also, by the strain shaking table culture 8 days of comparative example, so that its more abundant bacterium germination.
The bacterium germination and harvesting situation of comparing embodiment and comparative example are as a result as follows：Embodiment shaking table culture 6 days, comparative example
Shaking table culture 8 days, but the bacterium ball of embodiment is still substantially denser than the bacterium ball of comparative example, evenly.Compare annesl time, implement
The later stage of example inoculation needs 4 days annesls, and the later stage of comparative example inoculation needs 6 days annesls.Finally compare the yield of harvesting, implement
The average product of example is 300g/ boxes, and the average product of comparative example is 270g/ boxes.
The preferred embodiments of the invention are the foregoing is only, are not intended to limit the invention, for the science and technology of this area
Personnel, the present invention, which can have, more to be changed and change, any modification made within the spirit and principles of the invention, etc.
It is all contained in replacing, improving within the present invention.
Claims (10)
1. a kind of breeding method of Cordyceps militaris strain, including the pure culture of Cordyceps militaris is inoculated into fluid nutrient medium, shake
Bed culture obtains liquid spawn；The fluid nutrient medium in parts by weight, including：9-10 parts of potato, fresh silkworm chrysalis 9-10
The leachate of part, 0.8-1 parts of glucose, 0.8-1 parts of peptone, 0.8-1 parts of yeast extract, 0.09-0.1 parts of potassium dihydrogen phosphate,
0.04-0.05 parts of magnesium sulfate, 0.09-0.1 parts of degreased pupa powder, 0.0005-0.0006 parts of sodium hydroxide, 45-60 parts of water, dimension life
Plain B1 0.01-0.02 parts；The parts by weight of the water include the water in silkworm chrysalis leachate.
2. the breeding method of Cordyceps militaris strain according to claim 1, it is characterised in that the bar of the shaking table culture
Part is：With 200-250r/min shaking table cultures 5-6 days, cultivation temperature was 20-22 DEG C.
3. the breeding method of Cordyceps militaris strain according to claim 1, it is characterised in that the silkworm chrysalis leachate is
Fresh silkworm chrysalis is cooked to the silkworm chrysalis filtered fluid obtained after 25-35min.
4. the breeding method of Cordyceps militaris strain according to claim 1, it is characterised in that the albumen of the potato
Matter content is not less than 3%, and content of starch is not less than 13%, preferably 13%-15%.
5. the breeding method of Cordyceps militaris strain according to claim 1, it is characterised in that the total nitrogen of the peptone
Content is not less than 13.5%, and amino acid content is not less than 3%.
6. the breeding method of Cordyceps militaris strain according to claim 1, it is characterised in that the degreased pupa powder
Protein content is 60%-80%, and fat content is 5%-8%.
7. application of the breeding method of the Cordyceps militaris strain described in claim any one of 1-6 in Cordyceps militaris is cultivated.
8. a kind of method for cultivating Cordyceps militaris, it is characterised in that including：By the Cordyceps militaris liquid described in claim any one of 1-6
Liquid spawn prepared by the breeding method of body strain is forwarded to fructification culture medium, proceeds culture.
9. the method according to claim 8 for cultivating Cordyceps militaris, it is characterised in that by the used silkworm chrysalis of silkworm chrysalis leachate
It is put into fructification culture medium.
10. a kind of nutrient solution for cultivating Cordyceps militaris strain, in parts by weight, including：9-10 parts of potato, fresh silkworm chrysalis
9-10 parts of leachate, 0.8-1 parts of glucose, 0.8-1 parts of peptone, 0.8-1 parts of yeast extract, potassium dihydrogen phosphate 0.09-0.1
Part, 0.04-0.05 parts of magnesium sulfate, 0.09-0.1 parts of degreased pupa powder, 0.0005-0.0006 parts of sodium hydroxide, 45-60 parts of water,
0.01-0.02 parts of vitamin B1；The parts by weight of the water include the water in silkworm chrysalis leachate.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108653782A (en) * | 2018-05-18 | 2018-10-16 | 翟琳 | A kind of preparation method of air freshener |
| CN109328875A (en) * | 2018-11-05 | 2019-02-15 | 辽宁省农业科学院 | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch |
| CN109618815A (en) * | 2019-01-18 | 2019-04-16 | 福州隆利信生物制品有限公司 | The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice |
| CN112831420A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Cordyceps militaris liquid strain culture medium and strain culture method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN101173218A (en) * | 2007-09-30 | 2008-05-07 | 袁有宝 | Cultivate method for cordyceps militaris link |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
| CN103125275A (en) * | 2013-03-15 | 2013-06-05 | 熊艳 | Cultivation method of cordyceps militaris |
-
2017
- 2017-05-20 CN CN201710360091.3A patent/CN107058134A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN101173218A (en) * | 2007-09-30 | 2008-05-07 | 袁有宝 | Cultivate method for cordyceps militaris link |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
| CN103125275A (en) * | 2013-03-15 | 2013-06-05 | 熊艳 | Cultivation method of cordyceps militaris |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108653782A (en) * | 2018-05-18 | 2018-10-16 | 翟琳 | A kind of preparation method of air freshener |
| CN109328875A (en) * | 2018-11-05 | 2019-02-15 | 辽宁省农业科学院 | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch |
| CN109618815A (en) * | 2019-01-18 | 2019-04-16 | 福州隆利信生物制品有限公司 | The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice |
| CN109618815B (en) * | 2019-01-18 | 2021-04-16 | 福州隆利信生物制品有限公司 | Method for culturing cordyceps sinensis mycelia by using red yeast rice extraction residues |
| CN112831420A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Cordyceps militaris liquid strain culture medium and strain culture method thereof |
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