CN112831420A - Cordyceps militaris liquid strain culture medium and strain culture method thereof - Google Patents
Cordyceps militaris liquid strain culture medium and strain culture method thereof Download PDFInfo
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- CN112831420A CN112831420A CN201911168112.7A CN201911168112A CN112831420A CN 112831420 A CN112831420 A CN 112831420A CN 201911168112 A CN201911168112 A CN 201911168112A CN 112831420 A CN112831420 A CN 112831420A
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- cordyceps militaris
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 46
- 239000007788 liquid Substances 0.000 title claims abstract description 34
- 239000001963 growth medium Substances 0.000 title claims abstract description 26
- 238000012136 culture method Methods 0.000 title description 5
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 17
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 17
- 241000209140 Triticum Species 0.000 claims abstract description 17
- 235000021307 Triticum Nutrition 0.000 claims abstract description 17
- 241000255789 Bombyx mori Species 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 239000001888 Peptone Substances 0.000 claims abstract description 9
- 108010080698 Peptones Proteins 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- 235000012054 meals Nutrition 0.000 claims abstract description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 9
- 235000019319 peptone Nutrition 0.000 claims abstract description 9
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 9
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 9
- 239000008399 tap water Substances 0.000 claims abstract description 9
- 235000020679 tap water Nutrition 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 235000012015 potatoes Nutrition 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 abstract description 5
- 230000007850 degeneration Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 5
- 206010011224 Cough Diseases 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000221775 Hypocreales Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 206010041497 Spermatorrhoea Diseases 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a cordyceps militaris liquid strain culture medium which comprises the following raw materials in parts by weight: 2-3 parts of potato, 0.1-0.2 part of wheat, 0.1-0.3 part of glucose, 0.05-0.15 part of monopotassium phosphate, 0.3-0.5 part of peptone, 0.5-1.0 part of silkworm chrysalis meal and 10 parts of tap water. The cordyceps militaris liquid strain obtained by the method has the advantages of high strain growing speed, strong activity, high grass yield and low strain degeneration rate, and can avoid the culture risk.
Description
Technical Field
The invention belongs to the technical field of cordyceps militaris cultivation, and particularly relates to a cordyceps militaris liquid strain culture medium and a strain culture method thereof.
Background
Cordyceps militaris, pupa of a multiply-infected lepidopteran insect, is a complex composed of a stroma (i.e., a grass part, also called a fruiting body) and a sclerotium (i.e., a corpse part of the insect), and simply speaking, is the combination of the body and the grass. Cordyceps militaris is a model species of Ascomycota, Hypocreales, Clavicipitaceae, Cordyceps. The scientific name is Cordycepsmitaris (L.exFr.) Link, Cordyceps militaris, called Cordyceps militaris, or Cordyceps militaris for short, generally the Cordyceps militaris cultured by living pupa is called Cordyceps militaris, the two are the same kind of fungi, but the content difference of the two is large. Cordyceps militaris is distributed worldwide, and the quantity of natural resources is small.
The traditional Chinese medicine considers that the cordyceps militaris can tonify both lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, waist and knee soreness, weakness after illness, chronic cough and weakness, cough with phlegm and blood caused by overexertion, spontaneous perspiration and night sweat and the like, and is a traditional Chinese medicine capable of balancing and regulating yin and yang simultaneously.
The biggest problem encountered in large-scale cultivation of cordyceps militaris is that the strain degeneration is fast, and the cordyceps militaris liquid strain obtained by the method has the advantages of fast strain growing speed, strong activity, high grass yield, extremely low strain degeneration rate and capability of avoiding cultivation risks.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a cordyceps militaris liquid strain culture medium and a strain culture method thereof.
A cordyceps militaris liquid strain culture medium comprises the following raw materials in parts by weight:
2-3 parts of potato, 0.1-0.2 part of wheat, 0.1-0.3 part of glucose, 0.05-0.15 part of monopotassium phosphate, 0.3-0.5 part of peptone, 0.5-1.0 part of silkworm chrysalis meal and 10 parts of tap water.
The preferred technical scheme of the invention is as follows:
a cordyceps militaris liquid strain culture medium comprises the following raw materials in parts by weight: 2 parts of potato, 0.1 part of wheat, 0.2 part of glucose, 0.1 part of monopotassium phosphate, 0.4 part of peptone, 0.8 part of silkworm chrysalis meal and 10 parts of tap water.
The preparation method of the cordyceps militaris liquid strain culture medium sequentially comprises the following steps:
(1) peeling potatoes, cutting into blocks, boiling the blocks in water for 30 minutes, and taking supernatant for later use, wherein the ratio of the potatoes to the water is 1: 5;
(2) boiling wheat in water for 30 minutes, and taking supernatant for later use, wherein the ratio of the wheat to the water is 1: 5;
(3) mixing the supernatant of the above materials, adding other materials, and mixing.
The cordyceps militaris liquid strain culture method sequentially comprises the following steps:
(1) mixing the components of the cordyceps militaris liquid strain culture medium, filling the liquid culture medium in a triangular flask, sterilizing at the temperature of 120 ℃ for 30-40 minutes at 100 ℃, and cooling to normal temperature;
(2) inoculating the mother strain of Cordyceps militaris into a liquid bottle, standing for 20-30 hours at the early stage, adjusting to 120-phase rotation for 150 turns at the later stage, and performing constant-temperature and uniform-speed rotation cultivation in a dark room for 90-100 hours to obtain the liquid strain of Cordyceps militaris, which can be used for large-scale quantitative cultivation.
The cordyceps militaris liquid strain obtained by the method has the advantages of high strain growing speed, strong activity, high grass yield and extremely low strain degradation rate, and can avoid the risk of cultivation.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
A cordyceps militaris liquid strain culture medium comprises the following components in parts by weight: 2 parts of potato, 0.1 part of wheat, 0.2 part of glucose, 0.1 part of monopotassium phosphate, 0.4 part of peptone, 0.8 part of silkworm chrysalis meal and 10 parts of tap water.
Example 2
A cordyceps militaris liquid strain culture medium comprises the following components in parts by weight: 2 parts of potato, 0.15 part of wheat, 0.3 part of glucose, 0.07 part of monopotassium phosphate, 0.4 part of peptone, 0.8 part of silkworm chrysalis meal and 10 parts of tap water.
Example 3
A cordyceps militaris liquid strain culture medium comprises the following components in parts by weight: 2 parts of potato, 0.15 part of wheat, 0.1 part of glucose, 0.15 part of monopotassium phosphate, 0.5 part of peptone, 0.7 part of silkworm chrysalis meal and 10 parts of tap water.
Comparative example
A cordyceps militaris liquid strain culture medium is prepared from the following components in parts by weight: a cordyceps militaris liquid strain culture medium comprises the following components in parts by weight: 2 parts of potato, 0.1 part of wheat, 0.2 part of glucose, 0.1 part of monopotassium phosphate, 0.4 part of peptone, 0.8 part of silkworm chrysalis meal and 10 parts of tap water; the comparative example is different from example 1 in that potatoes are cut into small pieces, and wheat is made into wheat flour as a solid medium.
The preparation method of the cordyceps militaris liquid strain culture medium in the embodiment and the comparative example sequentially comprises the following steps of:
(1) peeling potatoes, cutting into blocks, boiling the blocks in water for 30 minutes, and taking supernatant for later use, wherein the ratio of the potatoes to the water is 1: 5;
(2) boiling wheat in water for 30 minutes, and taking supernatant for later use, wherein the ratio of the wheat to the water is 1: 5;
(3) mixing the supernatant of the above materials, adding other materials, and mixing.
Application examples
The culture mediums of the embodiment 1 and the comparative example are used for culturing cordyceps militaris liquid strains, and the embodiment 1 sequentially comprises the following steps:
(1) mixing the components of the cordyceps militaris liquid strain culture medium, filling the liquid culture medium into a triangular flask, sterilizing at 120 ℃ for 30 minutes, and cooling to normal temperature;
(2) inoculating the mother strain of Cordyceps militaris into a liquid bottle, standing for 24 hours at the early stage, adjusting to 14 turns at the later stage, and carrying out constant-temperature and uniform-speed rotary cultivation in a dark room for 96 hours to obtain Cordyceps militaris liquid strain, which can be used for large-scale quantitative cultivation.
The comparative example adopts solid culture medium for inoculation, the cordyceps militaris strain can be obtained after 120 hours, and the strain is easy to degenerate.
The above examples are merely examples for clearly illustrating the present application and do not limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.
Claims (6)
1. A cordyceps militaris liquid strain culture medium is characterized by comprising the following raw materials in parts by weight: 2-3 parts of potato, 0.1-0.2 part of wheat, 0.1-0.3 part of glucose, 0.05-0.15 part of monopotassium phosphate, 0.3-0.5 part of peptone, 0.5-1.0 part of silkworm chrysalis meal and 10 parts of tap water.
2. The cordyceps militaris liquid strain culture medium according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 2 parts of potato, 0.1 part of wheat, 0.2 part of glucose, 0.1 part of monopotassium phosphate, 0.4 part of peptone, 0.8 part of silkworm chrysalis meal and 10 parts of tap water.
3. The cordyceps militaris liquid strain culture medium according to claim 1, wherein the preparation method sequentially comprises the following steps:
(1) peeling potatoes, cutting into blocks, boiling the potato blocks in water for 30 minutes, and taking supernatant for later use;
(2) boiling wheat in water for 30 min to obtain supernatant;
(3) mixing the supernatant of the above materials, adding other materials, and mixing.
4. The cordyceps militaris liquid strain culture medium according to claim 3, wherein the ratio of the potatoes to the water in the step (1) is 1: 5.
5. The cordyceps militaris liquid strain culture medium according to claim 3, wherein the ratio of wheat to water in the step (1) is 1: 5.
6. A method for culturing Cordyceps militaris liquid strains is characterized by sequentially comprising the following steps:
(1) mixing the components of the cordyceps militaris liquid strain culture medium, filling the liquid culture medium in a triangular flask, sterilizing at the temperature of 120 ℃ for 30-40 minutes at 100 ℃, and cooling to normal temperature;
(2) inoculating the mother strain of Cordyceps militaris into a liquid bottle, standing for 20-30 hours at the early stage, adjusting to 120-phase rotation for 150 turns at the later stage, and carrying out constant-temperature and uniform-speed rotation cultivation in a dark room for 90-100 hours to obtain the liquid strain of Cordyceps militaris.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911168112.7A CN112831420A (en) | 2019-11-25 | 2019-11-25 | Cordyceps militaris liquid strain culture medium and strain culture method thereof |
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| CN201911168112.7A CN112831420A (en) | 2019-11-25 | 2019-11-25 | Cordyceps militaris liquid strain culture medium and strain culture method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114051889A (en) * | 2021-11-18 | 2022-02-18 | 湖南金芙农业科技有限公司 | Cordyceps militaris liquid culture medium for promoting rapid development of fruiting bodies and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
Non-Patent Citations (1)
| Title |
|---|
| 张卓然等: "北方地区蛹虫草人工栽培技术研究初探", 《中国林副特产》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114051889A (en) * | 2021-11-18 | 2022-02-18 | 湖南金芙农业科技有限公司 | Cordyceps militaris liquid culture medium for promoting rapid development of fruiting bodies and preparation method thereof |
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Application publication date: 20210525 |