CN107333562A - A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris - Google Patents
A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris Download PDFInfo
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- CN107333562A CN107333562A CN201710318518.3A CN201710318518A CN107333562A CN 107333562 A CN107333562 A CN 107333562A CN 201710318518 A CN201710318518 A CN 201710318518A CN 107333562 A CN107333562 A CN 107333562A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention provides a kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, incubation is similar to traditional handicraft, but by setting specific temperature control value and light application time, improve the yield of Cordyceps militaris, it is higher in the medical value for the cordycepin for using the cordycepin content for improving fruiting bodies of cordyceps militaris in the case of natural material, making to extract in the culture medium of the present invention.
Description
Technical field
The present invention relates to a kind of cultural method, more particularly to a kind of culture side of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris
Method and technology.
Background technology
Cordyceps militaris is called northern Chinese caterpillar Fungus, Cordceps militaris, the effect of Cordyceps militaris：Tonifying lung is cloudy, kidney-replenishing, and the effect of Cordyceps militaris is
Kidney deficiency is controlled, impotence and seminal emission, soreness of waist and knee joint, eak after being ill, the traditional Chinese medical science thinks that it serves and strengthened the body resistance to consolidate the constitution, to chronic senile bronchus
Scorching, cor pulmonale has significant curative effect, can improve liver detoxification ability, plays liver protection effect, improves the antiviral and anti-spoke of body
Penetrate ability.
The traditional Chinese medical science thinks that cordyceps sinensis enters lung kidney two warp, can tonifying lung it is cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission, waist and knee
Ache, eak after being ill, chronic cough is weak, phthisical cough phlegm blood, spontaneous sweating etc., is that unique a kind of can simultaneously balance, adjust negative and positive
Chinese medicine.
Prior art is to the addition of the raw material by chemical treatment synthesis in the culture of Cordyceps militaris, in Cordyceps militaris is extracted
The effect of cordycepin is reduced during cordycepin, while yield is relatively low in incubation, can not meet the demand in market.
The content of the invention
The present invention provides a kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris and supports technology.To realize the present invention's
Purpose, technical scheme are as follows：
A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, the cultural method of the fruiting bodies of cordyceps militaris are coerced for light temperature
The method of compeling, the light temperature stress method are carried out according to following incubation step：
1）, culture medium crushing：Medium powder is broken into by 20-50 mesh using industrial pulverizer or juice extractor；
2）, sterilization, cooling, inoculation：High-temperature sterilization is carried out to the culture medium after crushing using pasteurization, is cooled to after sterilization
Normal temperature, aseptically access the strain of Cordyceps militaris；
3）, without optical culture：Cultivated 6-7 days in the casing of dull thread, until the surface of culture medium grows mycelia；
4）, dim light, thermophilic culture：Illumination 300lx, culture 3-6 days of 20 DEG C of temperature and holding illumination 16 daily are small in indoor greenhouse
When, annesl is completed, induction button produces；
5）, middle light, thermophilic culture：Illumination 300lx, the culture 14 days of 20 DEG C of temperature and holding illumination 12 daily are small in indoor greenhouse
When, promote sporophore growth；
6）, Qiang Guang, thermophilic culture：Illumination 1000lx, the culture 7 days of 22 DEG C of temperature and holding illumination 20 daily are small in indoor greenhouse
When, induction cordycepin production；
7), middle light, thermophilic culture：Illumination 500lx, 22 DEG C of temperature are cultivated 14 days and keep illumination 8 hours daily in indoor greenhouse,
Fructification biomass largely accumulates；
8), Qiang Guang, high-temperature cultivation：Illumination 1000lx, 26 DEG C of temperature are cultivated 7 days and keep illumination 8 hours daily in indoor greenhouse,
Induce cordycepin production；
9), dim light, Low- temperature culture：Illumination 300lx, 18 DEG C of temperature are cultivated 7 days and keep illumination 4 hours daily in indoor greenhouse,
Induce cordycepin production；
10）, Qiang Guang, high-temperature cultivation：Illumination 1000lx, the culture 7 days of 28 DEG C of temperature and holding illumination 18 daily are small in indoor greenhouse
When, induction cordycepin production；
11）, harvest fructification.
Preferably, the culture medium is according to weight meter, its component：Shelled peanut 95-105g, raw soya bean 95-105g, it is scarlet
Potato 95-105g, rice 490-510g, tomato 45-55g, dried silkworm chrysalis meal 45-55g, fresh beef 45-55g, fresh potato 40-50g, pupa
Cordyceps militaris sporocarp extract solution 45-55ml, pure water 990-1010ml.
Preferably, the preparation method of the fruiting bodies of cordyceps militaris extract solution is：Take fresh fruiting bodies of cordyceps militaris 4-6g, pure
Water 50-60ml is stirred, and then filter producing with filter screen.
The present invention provides a kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, incubation and traditional handicraft phase
Seemingly, but by setting specific temperature control value and light application time, the yield of Cordyceps militaris is improved, is being adopted in culture medium of the invention
With the cordycepin content that fruiting bodies of cordyceps militaris is improved in the case of natural material, make the medical value of cordycepin extracted more
It is high.
Embodiment
Scheme of the present invention is：
Embodiment one：
A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, the cultural method of the fruiting bodies of cordyceps militaris are coerced for light temperature
The method of compeling, the light temperature stress method are carried out according to following incubation step：
1）, culture medium crushing：Medium powder is broken into by 20 mesh using industrial pulverizer or juice extractor；
2）, sterilization, cooling, inoculation：High-temperature sterilization is carried out to the culture medium after crushing using pasteurization, is cooled to after sterilization
Normal temperature, aseptically access the strain of Cordyceps militaris；
3）, without optical culture：Cultivated 6 days in the casing of dull thread, until the surface of culture medium grows mycelia；
4）, dim light, thermophilic culture：Illumination 300lx, 20 DEG C of temperature are cultivated 3 days and keep illumination 16 hours daily in indoor greenhouse,
Annesl is completed, induction button produces；
5）, middle light, thermophilic culture：Illumination 300lx, the culture 14 days of 20 DEG C of temperature and holding illumination 12 daily are small in indoor greenhouse
When, promote sporophore growth；
6）, Qiang Guang, thermophilic culture：Illumination 1000lx, the culture 7 days of 22 DEG C of temperature and holding illumination 20 daily are small in indoor greenhouse
When, induction cordycepin production；
7), middle light, thermophilic culture：Illumination 500lx, 22 DEG C of temperature are cultivated 14 days and keep illumination 8 hours daily in indoor greenhouse,
Fructification biomass largely accumulates；
8), Qiang Guang, high-temperature cultivation：Illumination 1000lx, 26 DEG C of temperature are cultivated 7 days and keep illumination 8 hours daily in indoor greenhouse,
Induce cordycepin production；
9), dim light, Low- temperature culture：Illumination 300lx, 18 DEG C of temperature are cultivated 7 days and keep illumination 4 hours daily in indoor greenhouse,
Induce cordycepin production；
10）, Qiang Guang, high-temperature cultivation：Illumination 1000lx, the culture 7 days of 28 DEG C of temperature and holding illumination 18 daily are small in indoor greenhouse
When, induction cordycepin production；
11）, harvest fructification.
In the present embodiment, the culture medium is according to weight meter, its component：Shelled peanut 100g, raw soya bean 100g, it is scarlet
Potato 100g, rice 500g, tomato 50g, dried silkworm chrysalis meal 50g, fresh beef 50g, fresh potato 45g, fruiting bodies of cordyceps militaris extract solution
50ml, pure water 1000ml.
In the present embodiment, the preparation method of the fruiting bodies of cordyceps militaris extract solution is：Take fresh fruiting bodies of cordyceps militaris 5g,
Pure water 55ml is stirred, and then filter producing with filter screen.
The cordycepin content of fruiting bodies of cordyceps militaris：Average 3.5%, highest 5.34%, minimum 2.46%.
Embodiment two：
A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, the cultural method of the fruiting bodies of cordyceps militaris are coerced for light temperature
The method of compeling, the light temperature stress method are carried out according to following incubation step：
1）, culture medium crushing：Medium powder is broken into by 35 mesh using industrial pulverizer or juice extractor；
2）, sterilization, cooling, inoculation：High-temperature sterilization is carried out to the culture medium after crushing using pasteurization, is cooled to after sterilization
Normal temperature, aseptically access the strain of Cordyceps militaris；
3）, without optical culture：Cultivated 7 days in the casing of dull thread, until the surface of culture medium grows mycelia；
4）, dim light, thermophilic culture：Illumination 300lx, 20 DEG C of temperature are cultivated 6 days and keep illumination 16 hours daily in indoor greenhouse,
Annesl is completed, induction button produces；
5）, middle light, thermophilic culture：Illumination 300lx, the culture 12 days of 20 DEG C of temperature and holding illumination 12 daily are small in indoor greenhouse
When, promote sporophore growth；
6）, Qiang Guang, thermophilic culture：Illumination 1000lx, the culture 6 days of 22 DEG C of temperature and holding illumination 20 daily are small in indoor greenhouse
When, induction cordycepin production；
7), middle light, thermophilic culture：Illumination 500lx, 22 DEG C of temperature are cultivated 12 days and keep illumination 8 hours daily in indoor greenhouse,
Fructification biomass largely accumulates；
8), Qiang Guang, high-temperature cultivation：Illumination 1000lx, 26 DEG C of temperature are cultivated 6 days and keep illumination 8 hours daily in indoor greenhouse,
Induce cordycepin production；
9), dim light, Low- temperature culture：Illumination 300lx, 18 DEG C of temperature are cultivated 6 days and keep illumination 4 hours daily in indoor greenhouse,
Induce cordycepin production；
10）, Qiang Guang, high-temperature cultivation：Illumination 1000lx, the culture 6 days of 28 DEG C of temperature and holding illumination 18 daily are small in indoor greenhouse
When, induction cordycepin production；
11）, harvest fructification.
In the present embodiment, the culture medium is according to weight meter, its component：Its component is：Shelled peanut 95g, raw soya bean
95g, fresh sweet potatoes 95g, rice 490g, tomato 45g, dried silkworm chrysalis meal 45g, fresh beef 45g, fresh potato 40g, fruiting bodies of cordyceps militaris carry
Take liquid 45ml, pure water 990ml.
In the present embodiment, the preparation method of the fruiting bodies of cordyceps militaris extract solution is：Take fresh fruiting bodies of cordyceps militaris 4g,
Pure water 50ml is stirred, and then filter producing with filter screen.
Embodiment three：
A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, the cultural method of the fruiting bodies of cordyceps militaris are coerced for light temperature
The method of compeling, the light temperature stress method are carried out according to following incubation step：
1）, culture medium crushing：Medium powder is broken into by 50 mesh using industrial pulverizer or juice extractor；
2）, sterilization, cooling, inoculation：High-temperature sterilization is carried out to the culture medium after crushing using pasteurization, is cooled to after sterilization
Normal temperature, aseptically access the strain of Cordyceps militaris；
3）, without optical culture：Cultivated 6 days in the casing of dull thread, until the surface of culture medium grows mycelia；
4）, dim light, thermophilic culture：Illumination 300lx, 20 DEG C of temperature are cultivated 5 days and keep illumination 16 hours daily in indoor greenhouse,
Annesl is completed, induction button produces；
5）, middle light, thermophilic culture：Illumination 300lx, the culture 14 days of 20 DEG C of temperature and holding illumination 12 daily are small in indoor greenhouse
When, promote sporophore growth；
6）, Qiang Guang, thermophilic culture：Illumination 1000lx, the culture 7 days of 22 DEG C of temperature and holding illumination 20 daily are small in indoor greenhouse
When, induction cordycepin production；
7), middle light, thermophilic culture：Illumination 500lx, 22 DEG C of temperature are cultivated 14 days and keep illumination 8 hours daily in indoor greenhouse,
Fructification biomass largely accumulates；
8), Qiang Guang, high-temperature cultivation：Illumination 1000lx, 26 DEG C of temperature are cultivated 7 days and keep illumination 8 hours daily in indoor greenhouse,
Induce cordycepin production；
9), dim light, Low- temperature culture：Illumination 300lx, 18 DEG C of temperature are cultivated 7 days and keep illumination 4 hours daily in indoor greenhouse,
Induce cordycepin production；
10）, Qiang Guang, high-temperature cultivation：Illumination 1000lx, the culture 7 days of 28 DEG C of temperature and holding illumination 18 daily are small in indoor greenhouse
When, induction cordycepin production；
11）, harvest fructification.
In the present embodiment, the culture medium is according to weight meter, its component：Its component is：Shelled peanut 105g, raw soya bean
105g, fresh sweet potatoes 105g, rice 510g, tomato 55g, dried silkworm chrysalis meal 55g, fresh beef 55g, fresh potato 50g, fruiting bodies of cordyceps militaris
Extract solution 55ml, pure water 1010ml.
In the present embodiment, the preparation method of the fruiting bodies of cordyceps militaris extract solution is：Take fresh fruiting bodies of cordyceps militaris 6g,
Pure water 60ml is stirred, and then filter producing with filter screen.
It is the beneficial effect of the solution of the present invention compared with prior art below：
1. formula is rice 50g, nutrient solution 35g（Nutrient solution prescription be per 1000ml containing 15 ~ 20g of peptone, glucose 15 ~
20g, 20 ~ 30g of dried silkworm chrysalis meal, 20 ~ 30g of glycine, gland crash 2 ~ 3g of cry of certain animals, 1 ~ 2g of magnesium sulfate, 1 ~ 2g of potassium dihydrogen phosphate, dusty yeast 4 ~
5g, remaining is pure water）Culture medium use use following cultural method（1. fructification culture medium top layer forms small son
During entity, the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1% is sprayed again on culture medium top layer, fountain height is with firm
It is good that culture medium covering is defined；2. it is 18-20 DEG C to adjust temperature, daily illumination is no less than 12 hours, illumination 1500-200LX, training
Support 8-12 days；3. cultivating to the cultivation temperature of the culture of the 8-12 days and being adjusted to 18-25 DEG C of cultivation temperature, other conditions are constant,
Carry out cellar culture）, until harvesting fructification.Cordycepin content in gained fructification is 0.5-1.0%.And use the present invention
Cordycepin content in fructification obtained by the light temperature coercing cultivation method is 1.2-1.8%.
2. solid medium is made up of rice, silky ant powder and nutrient solution, the amount ratio of rice, silky ant powder and nutrient solution
Example is 40g：0.5g:20ml.Silky ant powder in solid medium is that 20 mesh sieves are crossed after silky ant is crushed, and the nutrient solution is every
Containing 20g peptones, 20g glucose, 1g potassium dihydrogen phosphates, 0.5g magnesium sulfate, 0.05g vitamins in liter, remaining is pure water.
Using following cultural method（1. fructification culture medium top layer forms small fructification, sprayed again on culture medium top layer
The gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1%, culture medium covering to be just defined by fountain height；2. adjustment temperature
Spend for 18-20 DEG C, daily illumination is no less than 12 hours, illumination 1500-2000LX, is cultivated 8-12 days；3. culture was to the 8-12 days
The cultivation temperature of culture be adjusted to 18-25 DEG C of cultivation temperature, other conditions are constant, carry out cellar culture）, until harvest is real
Body.Cordycepin content in gained fructification is 0.6-1.2%.And use light temperature coercing cultivation method resulting bottle of the present invention
Cordycepin content in entity is 1.5-2.5%.
The present invention provides a kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, incubation and traditional handicraft phase
Seemingly, but by setting specific temperature control value and light application time, the yield of Cordyceps militaris is improved, not in culture medium of the invention
The cordycepin content of fruiting bodies of cordyceps militaris is improved in the case of adding any raw material by chemical technology synthesis, makes to extract
Cordycepin medical value it is higher.
As known by the technical knowledge, the present invention can pass through the embodiment party of other essence without departing from its spirit or essential feature
Case is realized.Therefore, embodiment disclosed above, for each side, all it is merely illustrative, is not only.Institute
Have within the scope of the present invention or be included in the invention in the change being equal in the scope of the present invention.
Claims (3)
- A kind of 1. cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris, it is characterised in that the training of the fruiting bodies of cordyceps militaris The method of supporting is light temperature stress method, and the light temperature stress method is carried out according to following incubation step：1）, culture medium crushing：Medium powder is broken into by 20-50 mesh using industrial pulverizer or juice extractor；2）, sterilization, cooling, inoculation：High-temperature sterilization is carried out to the culture medium after crushing using pasteurization, is cooled to after sterilization Normal temperature, aseptically access the strain of Cordyceps militaris；3）, without optical culture：Cultivated 6-7 days in the casing of dull thread, until the surface of culture medium grows mycelia；4）, dim light, thermophilic culture：Illumination 300lx, culture 3-6 days of 20 DEG C of temperature and holding illumination 16 daily are small in indoor greenhouse When, annesl is completed, induction button produces；5）, middle light, thermophilic culture：Illumination 300lx, the culture 14 days of 20 DEG C of temperature and holding illumination 12 daily are small in indoor greenhouse When, promote sporophore growth；6）, Qiang Guang, thermophilic culture：Illumination 1000lx, the culture 7 days of 22 DEG C of temperature and holding illumination 20 daily are small in indoor greenhouse When, induction cordycepin production；7), middle light, thermophilic culture：Illumination 500lx, 22 DEG C of temperature are cultivated 14 days and keep illumination 8 hours daily in indoor greenhouse, Fructification biomass largely accumulates；8), Qiang Guang, high-temperature cultivation：Illumination 1000lx, 26 DEG C of temperature are cultivated 7 days and keep illumination 8 hours daily in indoor greenhouse, Induce cordycepin production；9), dim light, Low- temperature culture：Illumination 300lx, 18 DEG C of temperature are cultivated 7 days and keep illumination 4 hours daily in indoor greenhouse, Induce cordycepin production；10）, Qiang Guang, high-temperature cultivation：Illumination 1000lx, the culture 7 days of 28 DEG C of temperature and holding illumination 18 daily are small in indoor greenhouse When, induction cordycepin production；11）, harvest fructification.
- A kind of 2. cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris according to claim 1, it is characterised in that institute State culture medium is according to weight meter, its component：Shelled peanut 95-105g, raw soya bean 95-105g, fresh sweet potatoes 95-105g, rice 490-510g, tomato 45-55g, dried silkworm chrysalis meal 45-55g, fresh beef 45-55g, fresh potato 40-50g, fruiting bodies of cordyceps militaris extraction Liquid 45-55ml, pure water 990-1010ml.
- A kind of 3. cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris according to claim 1, it is characterised in that institute The preparation method for stating fruiting bodies of cordyceps militaris extract solution is：Fresh fruiting bodies of cordyceps militaris 4-6g, pure water 50-60ml is taken to be stirred Mix, then filter producing with filter screen.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
| CN112314325A (en) * | 2020-09-14 | 2021-02-05 | 上海国宝企业发展中心 | Method for culturing artificial cordyceps militaris sporocarp |
| CN112825730A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Method for cultivating cordyceps militaris with high adenosine content |
| CN117660579A (en) * | 2023-12-12 | 2024-03-08 | 中科药创(青岛)发酵工程有限公司 | Dual-drug-effect cordyceps militaris solid-state fermentation ginseng product and preparation method thereof |
-
2017
- 2017-05-08 CN CN201710318518.3A patent/CN107333562A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112825730A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Method for cultivating cordyceps militaris with high adenosine content |
| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
| CN112314325A (en) * | 2020-09-14 | 2021-02-05 | 上海国宝企业发展中心 | Method for culturing artificial cordyceps militaris sporocarp |
| CN117660579A (en) * | 2023-12-12 | 2024-03-08 | 中科药创(青岛)发酵工程有限公司 | Dual-drug-effect cordyceps militaris solid-state fermentation ginseng product and preparation method thereof |
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