CN112825730A - Method for cultivating cordyceps militaris with high adenosine content - Google Patents
Method for cultivating cordyceps militaris with high adenosine content Download PDFInfo
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Abstract
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps: (1) placing the culture medium inoculated with Cordyceps militaris strain in dark room culture room at earlier stage, culturing in normal dark room at 15-20 deg.C for 4-7 days, adjusting culture temperature to 15.5 deg.C, and humidity to 1 deg.C and 65-70%, culturing for 10-15 days, and allowing mycelia to penetrate the nutrient of the culture medium; (2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting a dry product to obtain high adenosine content which can reach more than 0.9%.
Description
Technical Field
The invention belongs to the technical field of cordyceps militaris cultivation, and particularly relates to a cordyceps militaris cultivation method with high cordycepin content.
Background
Cordyceps militaris, pupa of a multiply-infected lepidopteran insect, is a complex composed of a stroma (i.e., a grass part, also called a fruiting body) and a sclerotium (i.e., a corpse part of the insect), and simply speaking, is the combination of the body and the grass. Cordyceps militaris is a model species of Ascomycota, Hypocreales, Clavicipitaceae, Cordyceps. The scientific name is Cordycepsmitaris (L.exFr.) Link, Cordyceps militaris, called Cordyceps militaris, or Cordyceps militaris for short, generally the Cordyceps militaris cultured by living pupa is called Cordyceps militaris, the two are the same kind of fungi, but the content difference of the two is large. Cordyceps militaris is distributed worldwide, and the quantity of natural resources is small.
Adenosine is a cellular biological term, and refers to a compound in which N-9 of adenine and C-1 of D-ribose are linked by a β -glycosidic bond, and its phosphate is adenylic acid. Adenosine is an endogenous nucleoside distributed throughout human cells, can directly enter cardiac muscle to generate adenosine through phosphorylation, participates in cardiac muscle energy metabolism, and also participates in dilating coronary vessels to increase blood flow.
Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. Adenosine is an important intermediate for the synthesis of Adenosine Triphosphate (ATP), adenine, adenylate, and vidarabine.
According to the invention, the cordyceps militaris is cultured in a large scale, and a culture method for improving adenosine is found through multiple experiments for many years.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the cordyceps militaris cultivation method with high adenosine content.
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with Cordyceps militaris strain in dark room culture room at earlier stage, normally culturing in dark room at 15-20 deg.C for 4-7 days, adjusting culture temperature to 15.5 deg.C, and humidity to 1 deg.C and 65-70%, culturing for 10-15 days, and allowing mycelia to penetrate the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting a dry product to obtain high adenosine content which can reach more than 0.9%.
The preferred technical scheme of the invention is as follows:
a method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at about 18 ℃ for 7 days, adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and enabling hypha to thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting a dry product to obtain high adenosine content which can reach more than 0.9%.
The cordyceps militaris obtained by the method has high adenosine content and good quality.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at about 18 ℃ for 7 days, adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and enabling hypha to thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting a dry product to obtain high adenosine content which can reach more than 0.9%.
Example 2
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at about 18 ℃ for 5 days, then adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and enabling hypha to thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting a dry product to obtain high adenosine content which can reach more than 0.9%.
Comparative example 1
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at about 15.5 ℃ for 7 days, adjusting the culture temperature to 18 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and enabling hypha to thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to be about 22 ℃ and the humidity to be 65% in the first 7 days, adjusting the temperature to be 20 ℃ and the humidity to be 75% in the 8 th-16 th days, adjusting the temperature to be 23 ℃ and the humidity to be 80% in the 17 th-40 th days, harvesting when the sporocarp grows to 5-8 cm, and detecting the adenosine content to be 0.74% in a dry product.
Comparative example 2
A method for cultivating cordyceps militaris with high adenosine content sequentially comprises the following steps:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at about 18 ℃ for 7 days, adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and enabling hypha to thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to 22 ℃ or so and the humidity to 80% in the first 7 days, adjusting the temperature to 20 ℃ and the humidity to 75% in the 8 th-16 th days, adjusting the temperature to 23 ℃ and the humidity to 65% in the 17 th-40 th days, and harvesting when the sporocarp grows to 5-8 cm, wherein the adenosine content a obtained by dry product detection is 0.77%.
The above examples are merely examples for clearly illustrating the present application and do not limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.
Claims (3)
1. A method for cultivating cordyceps militaris with high adenosine content is characterized by sequentially comprising the following steps:
(1) placing the culture medium inoculated with Cordyceps militaris strain in dark room culture room at early stage, normally culturing in dark room at 15-20 deg.C for 4-7 days, adjusting culture temperature to 15.5 deg.C, and humidity to 1 deg.C and 65-70%, and culturing for 10-15 days;
(2) culturing mycelium on cross-shaped mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to 22 ℃ and the humidity to 65% in the first 7 days, adjusting the temperature to 20 ℃ and the humidity to 75% in the 8 th-16 th days, adjusting the temperature to 23 ℃ and the humidity to 80% in the 17 th-40 th days, and harvesting when the sporocarp grows to 5-8 centimeters.
2. The cultivation method of cordyceps militaris with high adenosine content according to claim 1, which comprises the following steps in sequence:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at 18 ℃ for 7 days, then adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and making hypha thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross-shaped mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to 22 ℃ and the humidity to 65% in the first 7 days, adjusting the temperature to 20 ℃ and the humidity to 75% in the 8 th-16 th days, adjusting the temperature to 23 ℃ and the humidity to 80% in the 17 th-40 th days, and harvesting when the sporocarp grows to 5-8 centimeters.
3. The cultivation method of cordyceps militaris with high adenosine content according to claim 1, which comprises the following steps in sequence:
(1) placing the culture medium inoculated with the cordyceps militaris strain into a darkroom culture room in the early stage, normally culturing in the darkroom at 18 ℃ for 5 days, then adjusting the culture temperature to 15.5 ℃, adjusting the temperature to be plus or minus 1 ℃, adjusting the humidity to be 70 percent, culturing for 10 days, and making hypha thoroughly eat the nutrient of the culture medium;
(2) culturing mycelium on cross-shaped mycelium on the surface of a culture medium cultured in a dark room under 200 lux white light, adjusting the temperature to 22 ℃ and the humidity to 65% in the first 7 days, adjusting the temperature to 20 ℃ and the humidity to 75% in the 8 th-16 th days, adjusting the temperature to 23 ℃ and the humidity to 80% in the 17 th-40 th days, and harvesting when the sporocarp grows to 5-8 centimeters.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627700A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for culturing solid cordyceps militars strain |
| CN102084780A (en) * | 2010-05-31 | 2011-06-08 | 陈卫东 | Method for culturing cordyceps militaris sporocarp with high-content bioactive substances |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104756763A (en) * | 2015-04-16 | 2015-07-08 | 刘晓红 | Method for improving content of cordycepin in cordyceps militaris fruiting bodies |
| CN107333562A (en) * | 2017-05-08 | 2017-11-10 | 李鹄鸣 | A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris |
| CN109429894A (en) * | 2018-11-06 | 2019-03-08 | 广东粤微生物科技有限公司 | The selenium-enriched cordceps militaris and its cultural method of a kind of high adenosine content and application |
-
2019
- 2019-11-25 CN CN201911168252.4A patent/CN112825730A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627700A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for culturing solid cordyceps militars strain |
| CN102084780A (en) * | 2010-05-31 | 2011-06-08 | 陈卫东 | Method for culturing cordyceps militaris sporocarp with high-content bioactive substances |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104756763A (en) * | 2015-04-16 | 2015-07-08 | 刘晓红 | Method for improving content of cordycepin in cordyceps militaris fruiting bodies |
| CN107333562A (en) * | 2017-05-08 | 2017-11-10 | 李鹄鸣 | A kind of cultural method of worm summer herb with high-content of cordycepin fruiting bodies of cordyceps militaris |
| CN109429894A (en) * | 2018-11-06 | 2019-03-08 | 广东粤微生物科技有限公司 | The selenium-enriched cordceps militaris and its cultural method of a kind of high adenosine content and application |
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