CN109618815A - The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice - Google Patents
The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice Download PDFInfo
- Publication number
- CN109618815A CN109618815A CN201910046307.8A CN201910046307A CN109618815A CN 109618815 A CN109618815 A CN 109618815A CN 201910046307 A CN201910046307 A CN 201910046307A CN 109618815 A CN109618815 A CN 109618815A
- Authority
- CN
- China
- Prior art keywords
- parts
- defective material
- red yeast
- yeast rice
- caterpillar fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses the methods for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice, extract defective material by the red yeast rice of natural fermented mistake, provide necessary microelement to the growth of cordyceps mycelia, and be easy to the nutrient of mycelium absorption and assimilation；The production and accumulation of cordycepin in cordyceps sinensis can be increased by the addition of vitamin；The amount that amino in cordyceps sinensis can be effectively increased by the addition of compound amino acid, plays facilitation to the synthesis of adenosine and cordycepin；A nutritional components in fermentation liquid can be made adequately to decompose, degrade by fermentation, reach the purpose of cordyceps mycelia rapid conversion；By blue, white light, alternately irradiation culture can effectively improve the polyoses content in Chinese caterpillar fungus hypha；By extracting the effective use of defective material to red yeast rice, reduce the processing cost of agricultural residue.
Description
Technical field
The present invention relates to cordyceps sinensis artificial breeding technique fields, and in particular to extracts defective material culture Cordyceps Militaris using using red yeast rice
The method of silk.
Background technique
With the continuous improvement of people's living standard and quality of life, the continuous expansion of cognition field, to functional health
The demand of product and food also increasingly increases.Cordyceps sinensis is a kind of medicinal and edible fungus with significant health care curative effect, wherein
Foremost is cordyceps sinensis, and relevant series of products are up to tens of kinds, including several major class such as health care product, food, Chinese patent drug
Product all enjoys high reputation in the whole world.But host of Cordyceps sinensis is single-minded, and living environment is harsh, and due to its natural host bat
Bat moth life cycle is long, natural enemy is more, disease is more, the larva yield wretched insufficiency of natural propagation, in addition in these years cordyceps sinensis
Environmental pollution is serious in producing region, and taste digging is adopted in artificial too early, excessively taste and grassy marshland is desertified, to cause immature Cordyceps Militaris sense
The chance of dye host such as largely reduces at the reasons, causes cordyceps sinensis quality and quantity sharp fall, and the source of goods is rare, and price is high
It is expensive, it is far from satisfying the growing demand in market.
So a large amount of artificially breeding Chinese caterpillar fungus occur pours into market, but in artificial culture, for cultivating Cordyceps Militaris
The culture medium of kind is unable to reach the growing environment of wild cordyceps sinensis, so can not obtain the micro of abundance in mycelial growth
Element, amino acid and vitamin etc. are far smaller than wild cordyceps sinensis, nothing so as to cause the active constituent in artificially breeding Chinese caterpillar fungus
Method reaches wild effect, is known as being adenosine and cordycepin and polysaccharide to the effective active composition of cordyceps sinensis at this stage.
Summary of the invention
It is an object of the present invention in view of the above shortcomings of the prior art, propose to extract defective material culture cordyceps sinensis using red yeast rice
The method of mycelia provides a kind of artificial culturing method with high-efficiency activated cordyceps sinensis.
The present invention proposes the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice, comprising the following steps:
(1), red yeast rice extracted into defective material drying, beat powder, obtain red kojic rice powder；
(2), by mass parts by 30-45 parts of red kojic rice powder, 10-15 parts of glucose, 7-10 parts of vitamin, 3-6 parts of compound amino acid,
0.5-1 parts of potassium dihydrogen phosphate, 0.5-1 parts of magnesium sulfate, 5-10 parts of yeast and 800-900 parts of water are mixed, and the 2-3d that ferments, and are obtained
To fermentation liquid；
(3), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam sterilization cabinet
In sterilize, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(4), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 3%-5% of corresponding culture medium weight, is connect
Shaking table culture is carried out after kind, cultivation temperature is 15-20 DEG C, and incubation time 8-12d obtains the liquid bacteria containing Chinese caterpillar fungus hypha
Kind.
Further, the red yeast rice extracts defective material and need to first be added after protease digested, at the inactivation for carrying out protease
It manages, adds cellulase after albumen enzyme-deactivating and digested.
Further, the red yeast rice also needs to be added 1-3 parts of fructose, 1-3 parts of starch and carbohydrase after extracting defective material enzymatic hydrolysis
1-2 parts.
Further, the vitamin is vitamin B1 and B6, and the mass ratio of the vitamin B1 and B6 are 1:1.
Further, the compound amino acid includes arginine 0.5-1 parts, 0.5-1 parts of lysine, histidine by mass parts
0.5-1 parts, 0.5-1 parts of threonine, 0.5-1 parts of aspartic acid and 0.5-1 parts of alanine.
Further, it is both needed to carry out sterile working in aseptic operation box in step (3) and (4).
Further, the latter 4 days progress illumination cultivations cultivated in step (4).
Further, illumination cultivation is that blue light replaces irradiation culture with white light, daily alternately primary.
Further, the blue light and white light are cold light source.
The method provided by the invention for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice, is mentioned by the red yeast rice of natural fermented mistake
Defective material is taken, necessary microelement is provided to the growth of cordyceps mycelia, and be easy to the nutrient of mycelium absorption and assimilation；It is logical
The addition for crossing vitamin can increase the production and accumulation of cordycepin in cordyceps sinensis；Can effectively it be increased by the addition of compound amino acid
The amount for adding amino in cordyceps sinensis plays facilitation to the synthesis of adenosine and cordycepin；By blue, white light, alternately irradiation culture can be with
Effectively improve the polyoses content in Chinese caterpillar fungus hypha；A nutritional components in fermentation liquid can be made adequately to decompose, drop by fermentation
Solution reaches the purpose of cordyceps mycelia rapid conversion by the effective use to red yeast rice extraction defective material, it is useless to reduce agricultural
The processing cost of material.
Specific embodiment
For present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate this hair
It is bright rather than limit the scope of the invention.In addition, various changes or modification that those skilled in the art make the present invention, this
A little equivalent forms are equally fallen in the application range claimed.Proportion in the embodiment of the present invention is by weight.
Embodiment 1
(1), red yeast rice extracted into defective material drying, beat powder, obtain red kojic rice powder；
(2), mass parts are pressed by 30 parts of red kojic rice powder, 10 parts of glucose, 7 parts of vitamin, 3 parts of compound amino acid, potassium dihydrogen phosphate
0.5 part, 0.5 part of magnesium sulfate, 5 parts of yeast and 800 parts of water are mixed, and the 2d that ferments, and obtain fermentation liquid；
Compound amino acid in the present embodiment includes 0.5 part of arginine, 0.5 part of lysine, 0.5 part of histidine, Soviet Union by mass parts
0.5 part of propylhomoserin, 0.5 part of aspartic acid and 0.5 part of alanine.
(3), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam and goes out
It sterilizes in bacterium cabinet, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(4), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 3% of corresponding culture medium weight, inoculation
After carry out shaking table culture, cultivation temperature is 15-20 DEG C, and incubation time 8d, latter 4 days progress illumination cultivations are obtained containing cordyceps sinensis
The liquid spawn of mycelia；
(5), liquid spawn is slowly injected by syringe into the barley worm of sterile drying, injection point 3-5, injection
Stop injection after to host surface humidity；
(6), it will cultivate in host apparatus culture bottle, and carry out unglazed dark culturing 30d, cultivation temperature is 15-20 DEG C；Light is carried out again
According to culture 35d, cultivation temperature be 26-30 DEG C to get arrive artificial aweto.
Embodiment 2
(1), red yeast rice extracted into defective material drying, beat powder, obtain red kojic rice powder；
(2), mass parts are pressed by 37 parts of red kojic rice powder, 13 parts of glucose, 8 parts of vitamin, 6 parts of compound amino acid, potassium dihydrogen phosphate
0.7 part, 0.7 part of magnesium sulfate, 8 parts of yeast and 850 parts of water are mixed, and the 3d that ferments, and obtain fermentation liquid；
Compound amino acid in the present embodiment includes 1 part of arginine, 1 part of lysine, 1 part of histidine, threonine 1 by mass parts
Part, 1 part of aspartic acid and 1 part of alanine.
(3), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam and goes out
It sterilizes in bacterium cabinet, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(4), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 4% of corresponding culture medium weight, inoculation
After carry out shaking table culture, cultivation temperature is 15-20 DEG C, and incubation time 10d, latter 4 days progress illumination cultivations are obtained containing cordyceps sinensis
The liquid spawn of mycelia；
(5), liquid spawn is slowly injected by syringe into the barley worm of sterile drying, injection point 3-5, injection
Stop injection after to host surface humidity；
(6), it will cultivate in host apparatus culture bottle, and carry out unglazed dark culturing 30d, cultivation temperature is 15-20 DEG C；Light is carried out again
According to culture 35d, cultivation temperature be 26-30 DEG C to get arrive artificial aweto.
Embodiment 3
(1), red yeast rice extracted into defective material drying, beat powder, obtain red kojic rice powder；
(2), mass parts are pressed by 45 parts of red kojic rice powder, 15 parts of glucose, 10 parts of vitamin, 6 parts of compound amino acid, potassium dihydrogen phosphate
1 part, 1 part of magnesium sulfate, 10 parts of yeast and 900 parts of water are mixed, and the 3d that ferments, and obtain fermentation liquid；
Compound amino acid in the present embodiment includes 1 part of arginine, 1 part of lysine, 1 part of histidine, threonine 1 by mass parts
Part, 1 part of aspartic acid and 1 part of alanine.
(3), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam and goes out
It sterilizes in bacterium cabinet, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(4), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 5% of corresponding culture medium weight, inoculation
After carry out shaking table culture, cultivation temperature is 15-20 DEG C, and incubation time 12d, latter 4 days progress illumination cultivations are obtained containing cordyceps sinensis
The liquid spawn of mycelia；
(5), liquid spawn is slowly injected by syringe into the barley worm of sterile drying, injection point 3-5, injection
Stop injection after to host surface humidity；
(6), it will cultivate in host apparatus culture bottle, and carry out unglazed dark culturing 30d, cultivation temperature is 15-20 DEG C；Light is carried out again
According to culture 35d, cultivation temperature be 26-30 DEG C to get arrive artificial aweto.
Comparative example
(1), by mass parts by 45 parts of starch, 15 parts of glucose, 10 parts of vitamin, 6 parts of compound amino acid, 1 part of potassium dihydrogen phosphate,
1 part of magnesium sulfate, 10 parts of yeast and 900 parts of water are mixed, and the 3d that ferments, and obtain fermentation liquid；
Compound amino acid in the present embodiment includes 1 part of arginine, 1 part of lysine, 1 part of histidine, threonine 1 by mass parts
Part, 1 part of aspartic acid and 1 part of alanine.
(2), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam and goes out
It sterilizes in bacterium cabinet, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(3), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 5% of corresponding culture medium weight, inoculation
After carry out shaking table culture, cultivation temperature is 15-20 DEG C, and incubation time 12d, latter 4 days progress illumination cultivations are obtained containing cordyceps sinensis
The liquid spawn of mycelia；
(4), liquid spawn is slowly injected by syringe into the barley worm of sterile drying, injection point 3-5, injection
Stop injection after to host surface humidity；
(5), it will cultivate in host apparatus culture bottle, and carry out unglazed dark culturing 30d, cultivation temperature is 15-20 DEG C；Light is carried out again
According to culture 35d, cultivation temperature be 26-30 DEG C to get arrive artificial aweto.
Evaluation:
Embodiment 1, embodiment 2, embodiment 3, comparative example and wild Chinese caterpillar fungus are carried out to the content inspection of adenosine, cordycepin and polysaccharide
It surveys, testing result is shown in Table 1.
The assay of adenosine is measured according to the cordyceps sinensis quality standard of " Chinese Pharmacopoeia ", method particularly includes: with
Octadecylsilane chemically bonded silica is filler (C18), and with phosphate buffer, (pH6.5 takes 0.01mol/L sodium dihydrogen phosphate
68.5ml is mixed with 0.01mol/L disodium hydrogen phosphate 315mL)-methanol (85:15) be mobile phase；Detection wavelength is 260nm；Essence
It is close to weigh adenosine reference substance, and 90% methanol is added, solution of every 1mL containing 20 μ g is made to get reference substance solution is arrived；Reality is taken respectively
Example 1, embodiment 2, embodiment 3, comparative example and wild Chinese caterpillar fungus each 0.5g are applied, it is accurately weighed, it sets in stuffed conical flask, precision is added
90% methanol 10mL, close plug shake up, and weighed weight is heated to reflux 30min, lets cool, then mend weight, shake up, and filter, take subsequent filtrate,
Up to each sample solution；Reference substance solution and each 10 μ l of sample solution are drawn respectively, are injected liquid chromatograph, are passed through concentration ratio etc.
In peak area ratio, each sample adenosine content is calculated.
Cordycepin content detection, C18 chromatographic column, with acetonitrile-water (7: 93) for mobile phase, flow velocity 1.0mL/min, inspection
Survey 260 nm of wavelength.Accurately weighed each sample is set in 250 mL measuring bottles;Add 50% ethyl alcohol, 20 mL.Ultrasound (100 W of power, frequency
40 kHz of rate) 20 min of mechanical shaking extraction, it lets cool, adds 50% ethyl alcohol to scale.It is filtered through 0.5 μm of miillpore filter, as sample is molten
Liquid.The content of cordycepin in sample is calculated with external standard method.
The detection of polyoses content, measuring method: it is accurate to draw each sample treatment fluid 1.0mL, it sets in 10 mL colorimetric cylinders, adds
Distilled water is to 2.0mL.Blank control Guan Zeyong distilled water.Add 6% phenol solution 1.0mL in sample cell and blank tube, shakes up, so
After be rapidly added concentrated sulfuric acid 5.0mL, shake up, place 5 min at room temperature, set and heat 15 min in boiling water bath, after taking-up rapidly
It is cooled to room temperature, absorbance is surveyed at 490 nm.
By the data in table 1 it is found that adenosine, cordycepin and polysaccharide in the cordyceps sinensis of the application artificial culture are intended to obviously
Higher than comparative example, although its effective active composition is without wild Chinese caterpillar fungus height, relatively wild Chinese caterpillar fungus content, has
Certain substitutability, so the present invention has certain creativeness.
Exemplary illustration is carried out to the embodiment of the present invention above, but the content is only preferable implementation of the invention
Example, should not be considered as limiting the scope of the invention.All the changes and improvements etc. of all application ranges according to the present invention,
It should all fall within the scope of the patent of the present invention.
Claims (9)
1. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice, it is characterised in that: the following steps are included:
(1), red yeast rice extracted into defective material drying, beat powder, obtain red kojic rice powder；
(2), by mass parts by 30-45 parts of red kojic rice powder, 10-15 parts of glucose, 7-10 parts of vitamin, 3-6 parts of compound amino acid,
0.5-1 parts of potassium dihydrogen phosphate, 0.5-1 parts of magnesium sulfate, 5-10 parts of yeast and 800-900 parts of water are mixed, and the 2-3d that ferments, and are obtained
To fermentation liquid；
(3), fermentation liquid is subjected to residue filter, collects filtrate, filtrate is poured into container and is sealed, and is placed into steam sterilization cabinet
In sterilize, temperature is 121 DEG C, time 30min, obtains fluid nutrient medium after cooling；
(4), cordyceps species are seeded in fluid nutrient medium, each monomer inoculum concentration is the 3%-5% of corresponding culture medium weight, is connect
Shaking table culture is carried out after kind, cultivation temperature is 15-20 DEG C, and incubation time 8-12d obtains the liquid bacteria containing Chinese caterpillar fungus hypha
Kind.
2. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as described in claim 1, it is characterised in that: the red yeast rice
Extracting defective material need to first be added after protease digested, and carry out the inactivation treatment of protease, add fiber after albumen enzyme-deactivating
Plain enzyme is digested.
3. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as claimed in claim 2, it is characterised in that: the red yeast rice
Also need to be added 1-3 parts of fructose, 1-3 parts of starch and 1-2 parts of carbohydrase after extracting defective material enzymatic hydrolysis.
4. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as described in claim 1, it is characterised in that: the dimension life
Element is vitamin B1And B6, the vitamin B1And B6Mass ratio be 1:1.
5. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as described in claim 1, it is characterised in that: described compound
Amino acid includes arginine 0.5-1 parts, 0.5-1 parts of lysine, 0.5-1 parts of histidine, 0.5-1 parts of threonine, day by mass parts
0.5-1 parts and alanine 0.5-1 parts of aspartic acid.
6. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as described in claim 1, it is characterised in that: step (3)
(4) it is both needed to carry out sterile working in aseptic operation box in.
7. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as described in claim 1, it is characterised in that: step (4)
Latter 4 days progress illumination cultivations of middle culture.
8. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as claimed in claim 7, it is characterised in that: illumination cultivation
Replace irradiation culture with white light for blue light, it is daily alternately primary.
9. the method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice as claimed in claim 8, it is characterised in that: the blue light
It is cold light source with white light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910046307.8A CN109618815B (en) | 2019-01-18 | 2019-01-18 | Method for culturing cordyceps sinensis mycelia by using red yeast rice extraction residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910046307.8A CN109618815B (en) | 2019-01-18 | 2019-01-18 | Method for culturing cordyceps sinensis mycelia by using red yeast rice extraction residues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109618815A true CN109618815A (en) | 2019-04-16 |
| CN109618815B CN109618815B (en) | 2021-04-16 |
Family
ID=66061236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910046307.8A Active CN109618815B (en) | 2019-01-18 | 2019-01-18 | Method for culturing cordyceps sinensis mycelia by using red yeast rice extraction residues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109618815B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408511A (en) * | 2019-07-08 | 2019-11-05 | 山东中惠生物科技股份有限公司 | Utilize the preparation process of red koji fermentation filtered fluid production red yeast rice rice vinegar |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5526808A (en) * | 1978-08-12 | 1980-02-26 | Hokushin Kousan Yuugen | Artifical cultivation of cordyceps |
| KR20020087194A (en) * | 2001-05-14 | 2002-11-22 | 주식회사 엔에스케이텍 | Solid cultivation method of cordyceps sinensis |
| CN101531968A (en) * | 2008-05-04 | 2009-09-16 | 贵州大学 | Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation |
| CN102154121A (en) * | 2011-01-14 | 2011-08-17 | 杭州千岛湖星遥实业有限公司 | Method for preparing Cordyceps militaris Monascus spp. |
| CN102771314A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Method for culturing selenium-enriched cordyceps militaris |
| CN102845221A (en) * | 2012-09-14 | 2013-01-02 | 王云 | Fermentation method of Cordyceps sinensis |
| CN104145719A (en) * | 2014-09-04 | 2014-11-19 | 重庆市中药研究院 | Cordyceps sinensis mycelium fermentation production method |
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
-
2019
- 2019-01-18 CN CN201910046307.8A patent/CN109618815B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5526808A (en) * | 1978-08-12 | 1980-02-26 | Hokushin Kousan Yuugen | Artifical cultivation of cordyceps |
| KR20020087194A (en) * | 2001-05-14 | 2002-11-22 | 주식회사 엔에스케이텍 | Solid cultivation method of cordyceps sinensis |
| CN101531968A (en) * | 2008-05-04 | 2009-09-16 | 贵州大学 | Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation |
| CN102154121A (en) * | 2011-01-14 | 2011-08-17 | 杭州千岛湖星遥实业有限公司 | Method for preparing Cordyceps militaris Monascus spp. |
| CN102771314A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Method for culturing selenium-enriched cordyceps militaris |
| CN102845221A (en) * | 2012-09-14 | 2013-01-02 | 王云 | Fermentation method of Cordyceps sinensis |
| CN104145719A (en) * | 2014-09-04 | 2014-11-19 | 重庆市中药研究院 | Cordyceps sinensis mycelium fermentation production method |
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
Non-Patent Citations (1)
| Title |
|---|
| 王菊凤等: "蓝光诱导下蛹虫草胞内多糖的单糖组成及虫草素和虫草多糖含量分析", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408511A (en) * | 2019-07-08 | 2019-11-05 | 山东中惠生物科技股份有限公司 | Utilize the preparation process of red koji fermentation filtered fluid production red yeast rice rice vinegar |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109618815B (en) | 2021-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102100152B (en) | Artificial culture method and culture medium for fruiting bodies of cordyceps militaris | |
| CN104164367B (en) | Dried silkworm cordyceps militaris and culture method thereof | |
| CN104145719A (en) | Cordyceps sinensis mycelium fermentation production method | |
| CN104920065B (en) | A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing | |
| CN104013657A (en) | Post-fermentation extracting method of extracting saponin from American ginseng medicinal material | |
| CN104388322A (en) | Solid state fermentation method of lipid-lowering Monascus purpureus Zhang-MP | |
| CN106282028A (en) | Method for culturing cordyceps sinensis | |
| CN103621315B (en) | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution | |
| CN101195806A (en) | Industrial production method of cordyceps mushroom | |
| CN103688759A (en) | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers | |
| CN104756754B (en) | A kind of cultural method of Antrodia camphorata fructification | |
| CN110122193A (en) | A kind of cordyceps militaris plantation method of stable high-content polysaccharide | |
| TWI422680B (en) | Culture medium for culturing fruiting bodies of antrodia cinnamomea and method for culturing the same | |
| CN108184540A (en) | A kind of method that Cordyceps militaris is cultivated using live body Yellow meal worm larva as host | |
| CN109618815A (en) | The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice | |
| CN101824459B (en) | Method for promoting accumulation of triterpene in betula platyphylla suk. suspension cell by utilizing endophytic fungi elicitor | |
| CN105557312A (en) | Method for cultivating Cordyceps products by taking Tenebrio molitor (L.) larvae as carriers | |
| CN106034738B (en) | A kind of method with silkworm pupa as host artificial culture silkworm chrysalis cicada fungus | |
| CN1986827B (en) | Truffle polyose preparing process | |
| CN105602857B (en) | A kind of optimization method that wild cicada fungus liquid spawn is manually cultivated | |
| CN107142256A (en) | The method of the secondary mutation breeding of Cordyceps militaris ultraviolet | |
| CN108901587A (en) | A kind of solid culture method of cicada fungus | |
| CN106922386A (en) | A kind of artificial culture method of cicada fungus | |
| CN100564510C (en) | A kind of substratum of liquid deep fermentation for producing truffle polysaccharide | |
| CN106922387A (en) | A kind of artificial culture method of cicada fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |