CN108990704A - A kind of production method of golden fungus liquid strain - Google Patents

Abstract

The invention belongs to edible fungi liquid strain technical field, a kind of disclosed production method of golden fungus liquid strain is to choose a golden ears or side handles of a utensil entity, then make slant tube culture medium with wheat bran, sawdust, glucose, magnesium sulfate, agar；Then golden ear tissue is inoculated into Tube propagation base and obtains slant tube strain；It will be dispensed into bottle,suction after golden fungus liquid bacteria culture fluid sterilization treatment again, the strain in slant tube master clock be divided into fritter, then strain block is inoculated into bottle,suction；Bottle,suction is placed in electromagnetic swing bed upper culture again, obtains bottle,suction liquid original seed；Again by golden fungus liquid bacteria culture fluid into being put into sterilization treatment in fermentor, then bottle,suction liquid original seed is inoculated into fermentor；Filtrated air culture is passed through into fermentor again to get golden fungus liquid strain is arrived.The tremella liquid spawn that the present invention obtains has the advantages that sprouting is fast, vigor is high, pollution rate is low, when for cultivating golden ear, greatly shorten the production of hybrid seeds time.

Description

A kind of production method of golden fungus liquid strain

Technical field

The invention belongs to edible fungi liquid strain technical fields, are related to a kind of production method of golden fungus liquid strain.

Background technique

Currently, domestic edible mushroom tremella quality is good, nutritive value is high, and the production of domestic edible mushroom tremella kind is all adopted With solid spawn, solid spawn production procedure is that test tube species to original seed need 30-40 days, and original seed was by cultivar 40-50 days.With admittedly The production of body strain, the production cycle is long, and pollution rate is high, and strain cell age is inconsistent, sprouts slow.

Summary of the invention

In order to overcome the deficiencies of the prior art, accelerate the development of edible mushroom cause, the present invention provides a kind of golden fungus liquid strain Production method.

In order to achieve the above objectives, the present invention adopts the following technical scheme:

A kind of production method of golden fungus liquid strain, its step are as follows:

Step 1: production its ingredient of slant tube culture medium is as follows: 15-18 parts of wheat brans, 60-75 parts of sawdusts, 15-20 portions of grapes Sugar, 0.5-0.85 parts of magnesium sulfate, 20-25 parts of agar；

Step 2: the high-quality golden ear for choosing no disease and pests harm takes intermediate tissue, slant tube training is inoculated into sterile environment Support base on, and in 21 DEG C~25 DEG C constant temperature and humidity culture 7 days~10 days, obtain test tube strains；

Step 3: tremella fluid is made in 15-18 parts of wheat brans, 60-75 parts of sawdusts, 15-20 parts of glucose, 0.5-0.85 parts of magnesium sulfate Body shake flask culture, is then charged into bottle,suction, and 121-123 DEG C of high-temperature sterilization is handled 40 minutes, then inclined-plane parent species test tube is trained It supports the strain on base and is divided into 0.6cm*0.6cm piece bulk, be inoculated into suction filtration, connect in each bottle,suction bottle in an aseptic environment 4 pieces~6 ferfas kind blocks of kind；The bottle,suction uses 40~45min of ozone sterilization using preceding, then 22 DEG C~25 in dark surrounds DEG C constant temperature and humidity static gas wave refrigerator 72 hours~96 hours；

Step 4: the bottle,suction bottle after static gas wave refrigerator is placed on electromagnetic swing bed, 84 hours~96 are cultivated at 23 DEG C~25 DEG C Hour, obtain bottle,suction liquid original seed；

Step 5: fermentation tank culture liquid is by once at being grouped as: 10-20 parts of cornstarch, 10-15 parts of wheat brans, 20-22 portions of grapes Sugar, 10-14 portions of corn flour, 0.5-1 parts of potassium dihydrogen phosphates, 0.5-0.9 parts of magnesium sulfate will be put into fermentor with the culture solution postponed Then bottle,suction liquid original seed is inoculated into fermentor by middle sterilization treatment；

Step 6: being passed through constant temperature dehumidifying into fermentor treated filtrated air, the pressure after being passed through filtrated air is 0.02MPa~0.05MPa, air flow control are finally cultivated 96 hours~100 hours at 24 DEG C~25 DEG C in 0.5-1:1, It is fabricated to golden fungus liquid strain.

Due to using technical solution as above, the present invention is had the following advantages:

A kind of production method of golden fungus liquid strain proposed by the present invention, be choose first a golden ears or side handles of a utensil entity use again wheat bran, Sawdust, glucose, magnesium sulfate, agar make slant tube culture medium；Then golden ear tissue Tube propagation base is inoculated into obtain Slant tube strain；It will be dispensed into bottle,suction after golden fungus liquid bacteria culture fluid sterilization treatment again, by slant tube master clock Interior strain is divided into fritter, and then strain block is inoculated into bottle,suction；Bottle,suction is placed in electromagnetic swing bed upper culture again, Obtain bottle,suction liquid original seed；Bottle,suction liquid original seed is inoculated into fermentor again, is passed through filtrated air culture to get gold is arrived Ear liquid spawn.The tremella liquid spawn that this method obtains has the advantages that sprouting is fast, vigor is high, pollution rate is low, is being used for When cultivation gold ear, greatly shorten the production of hybrid seeds time.

Specific embodiment

A kind of production method of golden fungus liquid strain, its step are as follows: step 1 makes its ingredient of slant tube culture medium It is as follows: 20-28 parts of wheat brans, 65-70 parts of sawdusts, 16-22 portions of brown sugar, 0.6-1 parts of potassium dihydrogen phosphates, 20-25 parts of agar；Step 2 The high-quality Niu Jiner for choosing no disease and pests harm takes intermediate tissue, is inoculated on slant tube culture medium in sterile environment, and In 27 DEG C~29 DEG C constant temperature and humidity culture 6 days~9 days, obtain test tube strains；Step 3: by 20-28 parts of wheat brans, 65-70 parts of wood Bits, 16-22 portions of brown sugar, 0.6-1 parts of potassium dihydrogen phosphates, which are made golden fungus liquid culture solution and are fitted into 123-125 DEG C of high temperature in bottle,suction, to go out Strain on inclined-plane parent species Tube propagation base is being divided into 0.5cm*0.5cm bulk, is being inoculated into an aseptic environment by bacterium processing In suction filtration, 6 pieces~8 ferfas kind blocks are inoculated in each bottle,suction bottle；The bottle,suction uses 40~45min of ozone sterilization using preceding, Then 27 DEG C~29 DEG C constant temperature and humidity static gas wave refrigerator 3 days~5 days in dark surrounds；Step 5: the bottle,suction after static gas wave refrigerator Bottle is placed on electromagnetic swing bed, is cultivated 5 days~7 days at 26 DEG C~29 DEG C, is obtained bottle,suction liquid original seed；Step 6, fermentation Tank culture solution is by once at being grouped as: 10-25 parts of soybean powders, 15-18 parts of wheat brans, 16-22 parts of glucose, 12-16 portions of corn flour, 0.6-1 parts of potassium dihydrogen phosphates, 0.4-0.8 parts of magnesium sulfate will be put into sterilization treatment in fermentor with the culture solution postponed, then will Bottle,suction liquid original seed is inoculated into fermentor；Step 5: being passed through constant temperature dehumidifying into fermentor treated filtrated air, lead to Pressure after entering filtrated air is 0.03MPa~0.08MPa, and air flow control is in 0.6-1:1, finally at 26 DEG C~28 DEG C Culture 5 days~7 days, is fabricated to golden fungus liquid strain.

Liquid spawn only needs 17-22 days from test tube to fermentor.The liquid spawn of fermentor directly substitutes the cultivation of solid kind Cultivate use.Its sprouting is fast, vigor is high, pollution rate is low, with short production cycle, yield is high.

Claims (1)

1. a kind of production method of golden fungus liquid strain, which is characterized in that its step are as follows:

Step 1: production its ingredient of slant tube culture medium is as follows: 15-18 parts of wheat brans, 60-75 parts of sawdusts, 15-20 portions of grapes Sugar, 0.5-0.85 parts of magnesium sulfate, 20-25 parts of agar；

Step 2: the high-quality golden ear for choosing no disease and pests harm takes the tissue among stem, inclined-plane examination is inoculated into sterile environment On pipe culture medium, and in 21 DEG C~25 DEG C constant temperature and humidity culture 7 days~10 days, obtain test tube strains；

Step 3: tremella fluid is made in 15-18 parts of wheat brans, 60-75 parts of sawdusts, 15-20 parts of glucose, 0.5-0.85 parts of magnesium sulfate Body shake flask culture, is then charged into bottle,suction, and 121-123 DEG C of high-temperature sterilization is handled 40 minutes, then inclined-plane parent species test tube is trained It supports the strain on base and is divided into 0.6cm*0.6cm piece bulk, be inoculated into suction filtration, connect in each bottle,suction bottle in an aseptic environment 4 pieces~6 ferfas kind blocks of kind；The bottle,suction uses 40~45min of ozone sterilization using preceding, then 22 DEG C~25 in dark surrounds DEG C constant temperature and humidity static gas wave refrigerator 72 hours~96 hours；

Step 4: the bottle,suction bottle after static gas wave refrigerator is placed on electromagnetic swing bed, 84 hours~96 are cultivated at 23 DEG C~25 DEG C Hour, obtain bottle,suction liquid original seed；

Step 5: fermentation tank culture liquid is by once at being grouped as: 10-20 parts of cornstarch, 10-15 parts of wheat brans, 20-22 portions of grapes Sugar, 10-14 portions of corn flour, 0.5-1 parts of potassium dihydrogen phosphates, 0.5-0.9 parts of magnesium sulfate will be put into fermentor with the culture solution postponed Then bottle,suction liquid original seed is inoculated into fermentor by middle sterilization treatment；

Step 6: being passed through constant temperature dehumidifying into fermentor treated filtrated air, the pressure after being passed through filtrated air is 0.02MPa~0.05MPa, air flow control are finally cultivated 96 hours~100 hours at 24 DEG C~25 DEG C in 0.5-1:1, It is fabricated to golden fungus liquid strain.