CN101953267A - Method for culturing hepialus armoricanus oberthur cordyceps sinensis - Google Patents
Method for culturing hepialus armoricanus oberthur cordyceps sinensis Download PDFInfo
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- CN101953267A CN101953267A CN2009101441513A CN200910144151A CN101953267A CN 101953267 A CN101953267 A CN 101953267A CN 2009101441513 A CN2009101441513 A CN 2009101441513A CN 200910144151 A CN200910144151 A CN 200910144151A CN 101953267 A CN101953267 A CN 101953267A
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Abstract
The invention discloses a method for culturing hepialus armoricanus oberthur cordyceps sinensis, which comprises the following steps: (1) selecting primary high quality strain for breeding secondary liquid strain; (2) filling wheat and water in mass ratio of 4:3 into a container, sterilizing for 150 minutes at high pressure and high temperature, and then putting into a sterile room for illumination for 50 minutes to obtain a culture medium; (3) inoculating the secondary liquid strain on the culture medium in the container to obtain the strain carrying culture medium and sealing the container; (4) keeping the strain carrying culture medium at 20-22 DEG C for fermenting; (5) after cordyceps sinensis grows on the culture medium, opening the container for ventilating and supplying oxygen, keeping the concentration of carbon dioxide to be less than 0.5%, and keeping the temperature to be 16-20 DEG C; and (6) after the cordyceps sinensis grows for about 60 days, collecting to obtain the hepialus armoricanus oberthur cordyceps sinensis. The invention has simple production process, small investment, quick effect taking, high yield and low production cost, and the produced hepialus armoricanus oberthur cordyceps sinensis has high nutrient value.
Description
Technical field
The present invention relates to a kind of cultural method of Cordyceps sinensis, especially a kind of method of cultivating bat moth Chinese caterpillar fungus.
Background technology
Cordyceps militaris has another name called northern Chinese caterpillar Fungus, is called for short Cordyceps militaris.Be Ascomycotina, the Clavicipitaceae Cordyceps.Cordyceps militaris kind fungi is the type sepecies of Cordyceps, in China, the U.S., Canada, Italy, Japan, Germany, the former Soviet Union distribution is arranged all.But in natural output more than the Cordyceps sinensis rareness.Northern Chinese caterpillar Fungus is one of moth state Chinese caterpillar fungus kind distributed more widely, is rich in cordycepin, Cordyceps sinensis polysaccharide, cordycepic acid, also contains water-soluble or polar substances such as SOD enzyme, property of medicine action temperature and, medicine and the health care on multiple use is arranged.
Along with socioeconomic fast development, going from strength to strength of medical and health care system, the enhancing day by day of people's health care consciousness, the nutritive value and the health-care efficacy of Cordyceps sinensis are extensively approved, natural Cordyceps sinensis is favored deeply, but natural Cordyceps sinensis limited amount costs an arm and a leg, so the demand that artificial cultivation of cordyceps satisfies people has bigger social and economic significance.
Summary of the invention
Purpose of the present invention just provides a kind of method of cultivating bat moth Chinese caterpillar fungus, and the nutritive value of the bat moth Chinese caterpillar fungus that this method is turned out is used for replacing Cordyceps sinensis greater than Cordyceps sinensis, satisfies the demand of people to Cordyceps sinensis.
The technical solution used in the present invention is: a kind of method of cultivating bat moth Chinese caterpillar fungus is characterized in that it may further comprise the steps:
(1) choosing the one-level good quality strain breeds and makes second-class liquid isolate;
(2) in container by mass ratio pack at 4: 3 wheat and water, autoclave sterilization 150 minutes was put into desinfection chamber illumination 50 minutes again, made medium;
(3) second-class liquid isolate is inserted on the medium in the container, the medium that must carry disease germs, and container sealed;
(4) medium that carries disease germs is remained between 20 to 22 degrees centigrade ferment;
(5) after growing Chinese caterpillar fungus on the medium, open the container oxygen supply of leaking informaton, and keep 16 to 20 degrees centigrade of temperature, keep concentration of carbon dioxide less than 0.5%;
(6) treat that Chinese caterpillar fungus grew to about 60 days and begin to gather, bat moth Chinese caterpillar fungus.
Adopt technique scheme, second-class liquid isolate 2 weeks on medium can grow mycelia, and flavescence grows Chinese caterpillar fungus gradually, promptly get bat moth Chinese caterpillar fungus after gathering.
The invention has the beneficial effects as follows: production process is simple, invest little, instant effect, output height, low cost; The bat moth caterpillar fungus nutritire of system is worth high.
Embodiment
A kind of method of cultivating bat moth Chinese caterpillar fungus of the present invention, it may further comprise the steps:
(1) choosing the one-level good quality strain breeds and makes second-class liquid isolate;
(2) in container by mass ratio pack at 4: 3 wheat and water, autoclave sterilization 150 minutes was put into desinfection chamber illumination 50 minutes again, made medium;
(3) second-class liquid isolate is inserted on the medium in the container, the medium that must carry disease germs, and container sealed;
(4) medium that carries disease germs is remained between 20 to 22 degrees centigrade ferment;
(5) after growing Chinese caterpillar fungus on the medium, open the container oxygen supply of leaking informaton, keep concentration of carbon dioxide less than 0.5%, and keep 16 to 20 degrees centigrade of temperature;
(6) treat that Chinese caterpillar fungus grew to about 60 days and begin to gather, bat moth Chinese caterpillar fungus.
(7) Chinese caterpillar fungus under gathering is carried out freeze drying, pack with cobalt 60 irradiation warehouse-ins.
Claims (1)
1. method of cultivating bat moth Chinese caterpillar fungus is characterized in that it may further comprise the steps:
(1) choosing the one-level good quality strain breeds and makes second-class liquid isolate;
(2) in container by mass ratio pack at 4: 3 wheat and water, autoclave sterilization 150 minutes was put into desinfection chamber illumination 50 minutes again, made medium;
(3) second-class liquid isolate is inserted on the medium in the container, the medium that must carry disease germs, and container sealed;
(4) medium that carries disease germs is remained between 20 to 22 degrees centigrade ferment;
(5) after growing Chinese caterpillar fungus on the medium, open the container oxygen supply of leaking informaton, keep concentration of carbon dioxide less than 0.5%, and keep 16 to 20 degrees centigrade of temperature;
(6) treat that Chinese caterpillar fungus grew to about 60 days and begin to gather, bat moth Chinese caterpillar fungus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101441513A CN101953267A (en) | 2009-07-16 | 2009-07-16 | Method for culturing hepialus armoricanus oberthur cordyceps sinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101441513A CN101953267A (en) | 2009-07-16 | 2009-07-16 | Method for culturing hepialus armoricanus oberthur cordyceps sinensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101953267A true CN101953267A (en) | 2011-01-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101441513A Pending CN101953267A (en) | 2009-07-16 | 2009-07-16 | Method for culturing hepialus armoricanus oberthur cordyceps sinensis |
Country Status (1)
| Country | Link |
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| CN (1) | CN101953267A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498947A (en) * | 2011-11-07 | 2012-06-20 | 许继宏 | Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae |
| CN102523939A (en) * | 2012-03-16 | 2012-07-04 | 何寒 | Method for preparing cordyceps mycelium blocks by using germinated brown rice |
-
2009
- 2009-07-16 CN CN2009101441513A patent/CN101953267A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498947A (en) * | 2011-11-07 | 2012-06-20 | 许继宏 | Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae |
| CN102523939A (en) * | 2012-03-16 | 2012-07-04 | 何寒 | Method for preparing cordyceps mycelium blocks by using germinated brown rice |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110126 |