CN106973794B - A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof - Google Patents
A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof Download PDFInfo
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- 239000003104 tissue culture media Substances 0.000 title claims abstract description 61
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 34
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 34
- 230000001699 photocatalysis Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 58
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 52
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 32
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 32
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 32
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 29
- 239000008103 glucose Substances 0.000 claims abstract description 29
- 239000011780 sodium chloride Substances 0.000 claims abstract description 29
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 26
- 239000004471 Glycine Substances 0.000 claims abstract description 26
- 239000001110 calcium chloride Substances 0.000 claims abstract description 26
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 26
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 26
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 26
- 239000011941 photocatalyst Substances 0.000 claims abstract description 14
- 229940088710 antibiotic agent Drugs 0.000 claims abstract description 12
- 229940088594 vitamin Drugs 0.000 claims abstract description 6
- 229930003231 vitamin Natural products 0.000 claims abstract description 6
- 235000013343 vitamin Nutrition 0.000 claims abstract description 6
- 239000011782 vitamin Substances 0.000 claims abstract description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims description 47
- 229930003451 Vitamin B1 Natural products 0.000 claims description 27
- 229960003495 thiamine Drugs 0.000 claims description 27
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 27
- 235000010374 vitamin B1 Nutrition 0.000 claims description 27
- 239000011691 vitamin B1 Substances 0.000 claims description 27
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 24
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 24
- 229930003471 Vitamin B2 Natural products 0.000 claims description 24
- 229960002477 riboflavin Drugs 0.000 claims description 24
- 235000019164 vitamin B2 Nutrition 0.000 claims description 24
- 239000011716 vitamin B2 Substances 0.000 claims description 24
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims description 21
- 238000001816 cooling Methods 0.000 claims description 13
- 238000009835 boiling Methods 0.000 claims description 12
- 238000012856 packing Methods 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 10
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 7
- 239000011777 magnesium Substances 0.000 claims description 7
- 229910052749 magnesium Inorganic materials 0.000 claims description 7
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 5
- 239000005864 Sulphur Substances 0.000 claims description 5
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 4
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 4
- 235000019158 vitamin B6 Nutrition 0.000 claims description 4
- 239000011726 vitamin B6 Substances 0.000 claims description 4
- 229940011671 vitamin b6 Drugs 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 239000004408 titanium dioxide Substances 0.000 claims description 2
- 239000011787 zinc oxide Substances 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- 240000002853 Nelumbo nucifera Species 0.000 claims 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 46
- 241000894006 Bacteria Species 0.000 abstract description 23
- 230000012010 growth Effects 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 31
- 206010020649 Hyperkeratosis Diseases 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 230000008467 tissue growth Effects 0.000 description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 229910052791 calcium Inorganic materials 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 229930002875 chlorophyll Natural products 0.000 description 6
- 235000019804 chlorophyll Nutrition 0.000 description 6
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 6
- 230000008635 plant growth Effects 0.000 description 6
- 239000006259 organic additive Substances 0.000 description 5
- 235000019786 weight gain Nutrition 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000005660 chlorination reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000003630 growth substance Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 241000719837 Anoectochilus Species 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 241000026010 Dendrobium candidum Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000007146 photocatalysis Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 240000004638 Dendrobium nobile Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 238000003486 chemical etching Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000030968 tissue homeostasis Effects 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM) and preparation method thereof, the culture medium by weight, including the following raw material: ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, photocatalyst 0.1-0.5g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA0.02-0.03g, IAA0.02-0.03g, vitamin 0.04-0.08g, epsom salt 0.2-0.4g and pure water 80-100g.Before culture provided by the present invention is based on inoculated plant tissue, miscellaneous bacteria can be sufficiently killed under ultraviolet irradiation, and have to Plant Tissue Breeding and promote growth.
Description
Technical field
The invention belongs to field of tissue culture, specially a kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and its system
Preparation Method.
Background technique
Plant tissue culture media is the nutriment of plant tissue growth and maintenance manually prepared, and generally all contains inorganic battalion
Support object, carbon source, vitamin, growth regulatory substance and Organic additives etc..The culture medium for cultivating different plant cell tissues can root
According to actual needs, the necessary element of some plant cell growths is added.
Existing plant tissue culture media is in completion group assignment system and after being packed into narrow-mouthed bottle or culture dish, usually using cloth
Material or paper seal narrow-mouthed bottle bottleneck or the interface using ParafilmTM culture dish, and narrow-mouthed bottle is then put into high steam
Sterilization treatment 45-60min in autoclave, or by culture dish with paper bag live after, be put into sterilization treatment 45- in high-pressure steam sterilizing pan
60min.After the completion of high pressure steam sterilization, narrow-mouthed bottle is transferred in super-clean bench cooling, inoculation.
However above-mentioned plant tissue culture media sterilizing after the completion of be transferred to super-clean bench during, culture medium be easy by
Living contaminants.
Also, the one layer of bacterium that can only kill culture dish surface using ultraviolet light on the super-clean bench, can not be thoroughly to culture
Whole culture mediums in ware are thoroughly sterilized.When culture medium is contained using narrow-mouthed bottle, ultraviolet light can not be to intermediate in bottle
The culture medium at position is sterilized, and the plant tissue for causing living contaminants to be cultivated is unfavorable for plant tissue growth situation
Observation, miscellaneous bacteria also suck the nutrient in culture medium, play inhibiting effect to the growth of plant tissue.
Summary of the invention
The present invention provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), which helps growing plant
Tissue culture medium (TCM) solves existing culture medium and is sterilized by ultraviolet irradiation, can not kill asking for the miscellaneous bacteria at culture medium centre position
Topic.
To achieve the above object, the invention provides the following technical scheme:
A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM), by weight, including following composition: ammonium nitrate 0.8-
1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, photocatalyst 0.1-0.5g, calcium chloride
0.2-0.3g, glycine 0.04-0.06g, 6-BA0.02-0.03g, IAA0.02-0.03g, vitamin 0.04-0.08, seven water sulphur
Sour magnesium 0.2-0.4g and pure water 80-100g.
Wherein, contain the inorganic nutrients object needed in plant tissue culture course: epsom salt, chlorination in culture medium
Calcium, ammonium nitrate, potassium dihydrogen phosphate, sodium chloride；Carbon source: glucose；Organic additives: glycine and vitamin；Growth regulator:
IAA and 6-BA.Above-mentioned five major class nutriment is indispensable during plant tissue growth.
Meanwhile 0.8-1.2g ammonium nitrate provides sufficient nitrogen source for plant tissue growth, and it is thin can to effectively facilitate plant
The generation of protein, chlorophyll and nucleic acid in born of the same parents；0.4-0.6g potassium dihydrogen phosphate is also biomembrane in plant tissue, nucleotide
And the formation of nucleic acid provides sufficient P elements, and potassium element provided by potassium dihydrogen phosphate can promote nitrogen metabolism
And the transfer of carbohydrate, to accelerate the growth of plant tissue；0.2-0.3g calcium chloride is plant tissue cell's wall
Growth provide sufficient calcium constituent；In addition, the culture medium also added epsom salt as magnesium source, accelerate chlorophyll
It is formed；The Organic additives and growth regulator added in culture medium can promote plant tissue fast-growth.
Also, photocatalyst is also added in the culture medium, photocatalyst will not only inhibit the growth of plant cell, Er Qieke
With effectively kill be transferred to super-clean bench from high-pressure steam sterilizing pan during the microorganism that is infected, compensate for the purple in super-clean bench
Outer bactericidal assay can not kill the defect of miscellaneous bacteria inside culture medium.Miscellaneous bacteria is avoided to breed the life of interference experiment personnel observation of plant tissue
Long or miscellaneous bacteria, which absorbs the excessive nutrient of culture medium, leads to the generation that plant tissue growth is affected；
When culture medium be placed on open ultraviolet light super-clean bench under irradiation-sterilize when, photocatalyst can make full use of oxygen and
Water converts carbon dioxide and water for the miscellaneous bacteria in culture medium, to achieve the effect that sterilization；In addition, photocatalyst can also improve
Plant tissue photocatalysis efficiency promotes the plant tissue for having formed chlorophyll to carry out photosynthesis.
As further preferred, the bactericidal agent is one or both of nano-titanium dioxide, nano zine oxide.Nanometer
Titanium dioxide and nano zine oxide not only have a very high photocatalytic activity, and have acid-alkali-corrosive-resisting, resistant to chemical etching, nontoxic
The advantages that, price is also moderate, cost performance with higher.
Preferably, the antibacterial helps growing plant tissue culture medium (TCM) further include: at least one in vitamin B1, vitamin B6
Kind.Vitamin B6 has facilitation to the growth of root, and the generation of vitamin B1 and callus and viability have substantial connection.Cause
This, vitamin B1 and vitamin B6 are added in culture medium can effectively facilitate the growth of plant tissue.
Preferably, by weight, it includes: ammonium nitrate 0.8-1.2g, phosphorus that the antibacterial, which helps growing plant tissue culture medium (TCM),
It is acid dihydride potassium 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, nano-titanium dioxide 0.2-0.3g, nano oxidized
Zinc 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA 0.02-0.03g, IAA 0.02-0.03g, dimension
Raw element B1 0.02-0.04g, vitamin B2 0.02-0.04g, epsom salt 0.2-0.4g and pure water 80-100g.It is real
It verifies bright, takes product bactericidal effect obtained by the weight percentage more significant, the growth result of plant tissue is significant
It is promoted.
As further preferred, by weight, the antibacterial help growing plant tissue culture medium (TCM) include: ammonium nitrate 1.0g,
Potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide 0.25g, nano zine oxide 0.25g, calcium chloride
0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven water sulphur
Sour magnesium 0.3g and pure water 90g.
Preferably, it further includes agar that the antibacterial, which helps growing plant tissue culture medium (TCM),.Experimenter can be according to plant group
It knits and chooses whether addition agar in different growth ring sections.
The present invention also provides the preparation method that the antibacterial helps growing plant tissue culture medium (TCM), which includes:
(1) 80-100g pure water is added in container, is heated to boiling；
(2) take ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g,
Nano-titanium dioxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA
0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g sequentially add container
It is interior, and stir evenly, obtain mixed liquor；
(3) mixed liquor 0.2-0.4g epsom salt is adjusted into pH to 5.7, and with after conical flask packing and sealing, put
It is placed in high-pressure steam sterilizing pan the 45-60min that sterilizes, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Wherein, during (2) step prepares mixed liquor, according to above-mentioned order, after completely dissolution again to a kind of drug
Another drug is added.It is uniform using culture medium Nutrient distribution made from above-mentioned steps, and culture medium passes through high pressure steam sterilization
Pot sterilizing avoids miscellaneous bacteria from generating interference to the plant cell growth of required culture.Even if culture medium is from high pressure steam sterilization
Pot infects miscellaneous bacteria during being transferred to super-clean bench, and the photocatalyst contained in culture medium can use under ultraviolet irradiation
Miscellaneous bacteria is resolved into carbon dioxide and water by oxygen and moisture in culture medium, achievees the effect that degerming.
As further preferred, the preparation method specifically:
(1) 90g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide are taken
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, vitamin B2 0.03g are sequentially added in container, and are stirred evenly, and obtain mixed liquor；
(3) mixed liquor 0.3g epsom salt is adjusted into pH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 50min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
The present invention also provides above-mentioned antibacterials to help growing plant tissue culture medium (TCM) in radix tetrastigme, roxburgh anoectochilus terminal bud, rhizoma polygonati or iron sheet
Application in the tissue cultures such as dendrobium nobile.
Compared with prior art, the invention has the benefit that
(1) contain the inorganic nutrients object needed in plant tissue culture course: seven water in culture medium provided by the present invention
Magnesium sulfate, calcium chloride, ammonium nitrate, potassium dihydrogen phosphate, sodium chloride；Carbon source: glucose；Organic additives: glycine and vitamin；
Growth regulator: IAA and 6-BA.Above-mentioned five major class nutriment is indispensable during plant tissue growth；
Meanwhile 0.8-1.2g ammonium nitrate provides sufficient nitrogen source for plant tissue growth, and it is thin can to effectively facilitate plant
The generation of protein, chlorophyll and nucleic acid in born of the same parents；0.4-0.6g potassium dihydrogen phosphate is also biomembrane in plant tissue, nucleotide
And the formation of nucleic acid provides sufficient P elements, and potassium element provided by potassium dihydrogen phosphate can promote nitrogen metabolism
And the transfer of carbohydrate, to accelerate the growth of plant tissue；0.2-0.3g calcium chloride is plant tissue cell's wall
Growth provide sufficient calcium constituent；In addition, the culture medium also added epsom salt as magnesium source, accelerate chlorophyll
It is formed；The Organic additives and growth regulator added in culture medium can promote plant tissue fast-growth.
(2) photocatalyst is also added in culture medium provided by the present invention, photocatalyst will not only inhibit plant cell
Growth, and can effectively kill be transferred to super-clean bench from high-pressure steam sterilizing pan during the microorganism that is infected, compensate for
Ultraviolet-sterilization method in super-clean bench can not kill the defect of miscellaneous bacteria inside culture medium.Miscellaneous bacteria is avoided to breed interference experiment personnel observation
The growth of plant tissue or miscellaneous bacteria, which absorb the excessive nutrient of culture medium, leads to the generation that plant tissue growth is affected.
Specific embodiment
Technical solution of the present invention is described in further detail using specific embodiment below.
Embodiment 1
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, nano-titanium dioxide 0.2g, chlorination
Calcium 0.2g, glycine 0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, seven water sulphur
Sour magnesium 0.2g and pure water 80g.
Preparation method includes:
(1) 80g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, nano-titanium dioxide are taken
0.2g, calcium chloride 0.2g, glycine 0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2
0.02g, it sequentially adds in container, and stirs evenly, obtain mixed liquor；
(3) mixed liquor 0.2g epsom salt is adjusted into pH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 45min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Comparative example 1
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, calcium chloride 0.2g, glycine
0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, epsom salt 0.2g and pure
Water purification 80g.
Preparation method includes:
(1) 80g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, calcium chloride 0.2g, sweet ammonia are taken
Sour 0.04g, it 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, sequentially adds in container, and
It stirs evenly, obtains mixed liquor；
(3) mixed liquor 0.2g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 45min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Embodiment 2
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide 0.25g, chlorine
Change calcium 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven
Water magnesium sulfate 0.3g and pure water 90g.
Preparation method includes:
(1) 90g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide are taken
0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2
0.03g, it sequentially adds in container, and stirs evenly, obtain mixed liquor；
(3) mixed liquor 0.3g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Comparative example 2
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, calcium chloride 0.25g, glycine
0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, epsom salt 0.3g and
Pure water 90g.
Preparation method includes:
(1) 90g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, calcium chloride 0.25g, sweet ammonia are taken
Sour 0.05g, it 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, sequentially adds in container,
And stir evenly, obtain mixed liquor；
(3) mixed liquor 0.3g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Embodiment 3
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, nano-titanium dioxide 0.3g, chlorination
Calcium 0.3g, glycine 0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, seven water sulphur
Sour magnesium 0.4g and pure water 100g.
Preparation method includes:
(1) 100g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, nano-titanium dioxide are taken
0.3g, calcium chloride 0.3g, glycine 0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2
0.04g, it sequentially adds in container, and stirs evenly, obtain mixed liquor；
(3) mixed liquor 0.4g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 60min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Comparative example 3
By weight, the present embodiment provides a kind of photo-catalytic antibacterials to help growing plant tissue culture medium (TCM), by following substance group
At:
Ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, calcium chloride 0.3g, glycine
0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, epsom salt 0.4g and pure
Water purification 100g.
Preparation method includes:
(1) 100g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, calcium chloride 0.3g, sweet ammonia are taken
Sour 0.06g, it 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, sequentially adds in container, and
It stirs evenly, obtains mixed liquor；
(3) mixed liquor 0.4g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 60min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Embodiment 4
By weight, the present embodiment provides a kind of antibacterials to help growing plant tissue culture medium (TCM), by following material composition:
Ammonium nitrate 1.0g, it potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide 0.25g, receives
Rice zinc oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g,
Vitamin B2 0.03g, epsom salt 0.3g and pure water 90g.
Preparation method includes:
(1) 90g pure water is added in container, is heated to boiling；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide are taken
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, it vitamin B2 0.03g, sequentially adds in container, and stirs evenly, obtain mixed liquor；
(3) mixed liquor 0.3g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Embodiment 5
By weight, the present embodiment provides a kind of antibacterials to help growing plant tissue culture medium (TCM), by following material composition:
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano zine oxide 0.25g, chlorination
Calcium 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven water
Magnesium sulfate 0.3g and pure water 90g.
Preparation method includes:
(1) 90g pure water is added in container, is heated to boiling；
(2) take ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano zine oxide 0.25g,
Calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g,
It sequentially adds in container, and stirs evenly, obtain mixed liquor；
(3) mixed liquor 0.3g epsom salt is adjusted into PH to 5.7, and with after conical flask packing and sealing, be placed in
Sterilize 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
Comparative example 4
MS culture medium, preparation method and embodiment 2 are identical.
Comparative example 5
B5 medium, preparation method and embodiment 2 are identical.
Comparative example 6
N6 culture medium, preparation method and embodiment 2 are identical.
Test case 1
The present invention is applicable in the Plant Tissue Breeding of radix tetrastigme, roxburgh anoectochilus terminal bud, rhizoma polygonati or dendrobium candidum etc..Now it is cured with cultivating rhizoma polygonati
For injured tissue.
It is 1,2,3,4,5,6,7,8,9 that culture medium provided by embodiment 1-3 and comparative example 1-6 is numbered respectively, will be real
Applying the number of example 4 is 10, is 11 by the number of embodiment 5.The rhizoma polygonati callus of Zhejiang A & F University's Experimental Base plantation will be derived from
It is seeded in 1-11 group respectively, 20 parallel laboratory test groups of every group of setting；Meanwhile 1-11 group is correspondingly arranged blank control group respectively,
It is not inoculated with rhizoma polygonati callus, number A-K, 20 parallel laboratory test groups of every group of setting.
1-11 group and A-K group are cultivated 20 days under 26 ± 1 DEG C of poor light conditions after inoculation, observe more each culture medium Neihuang County
The growing state statistics of smart callus is as follows:
1 rhizoma polygonati callus growth situation statistical form of table
Bacterium colony generates situation in 2 blank control group A-I group culture medium of table
The rate of body weight gain of 1 1-3 group of table is all 84% or more, hence it is evident that is higher than 1 4-6 group of table, illustrates added by the present invention
Photocatalyst has apparent facilitation effect to the growth of rhizoma polygonati callus, the reason is that since photocatalyst promotion has formed leaf
The photocatalysis efficiency of the rhizoma polygonati callus of green element, to accelerate its growth；
10th group of rhizoma polygonati callus rate of body weight gain is higher than the reason of 1-3 group rhizoma polygonati callus: the tenth tissue culture
The combination in base using nano-titanium dioxide and nano zine oxide is supported, synergistic effect is played, promotes plant cell
Photosynthesis；
And the not high another reason of 4-6 group rate of body weight gain is: 4-6 group causes without addition photocatalyst with chlorophyll
Plant cell photosynthesis rate it is slow, cannot generate enough nutrients, thus it is slow to increase weight；By the weight gain of 1 1-3 group of table
Rate is higher than the rate of body weight gain of 7-9 group it is found that instant component is more suitable compared to MS culture medium, B5 medium, N6 culture medium
Rhizoma polygonati callus growth, and each component has synergistic function, promotes rhizoma polygonati callus rapid growth.
A-C and D-I group are compared in table 2, and the quantity that A-C group generates the culture medium of bacterium colony is significantly lower than D-I group,
It follows that the nano-titanium dioxide added in culture medium in the present invention has good bactericidal effect, and embodiment 2 is killed
Bacterium effect is best, while in the 10th group of blank control group J group, and the culture medium number for having bacterium colony to generate is 0, hence it is evident that less than real
Example 2 is applied, i.e. B group, reason is: using the combination of nano-titanium dioxide and nano zine oxide in J group culture medium, played
Synergistic effect, so that the culture medium bactericidal effect greatly increases.
It is greater than the quantity of the culture medium of A-C group generation bacterium by the quantity that K group generates the culture medium of bacterium and is given birth to by K group
It is less than the quantity that D-I group generates the culture medium of bacterium at the quantity of the culture medium of bacterium, can learns: in culture medium of the invention
The nano zine oxide of middle addition has preferable bactericidal effect, however bactericidal effect does not have nano-titanium dioxide and nano-silica
Change titanium, nano zine oxide combines；
According to test case 1 the result shows that: culture medium provided by the present invention is before inoculated plant tissue, in ultraviolet irradiation
Under can sufficiently kill miscellaneous bacteria, and have to the culture of rhizoma polygonati callus and promote growth.
In the same manner, the present invention is also suitable the culture of the plant tissues such as radix tetrastigme, roxburgh anoectochilus terminal bud or dendrobium candidum.
Claims (8)
1. a kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM), which is characterized in that by weight, including following component: nitre
Sour ammonium 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, photocatalyst 0.1-0.5g,
Calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA0.02-0.03g, IAA0.02-0.03g, vitamin 0.04-
0.08g, epsom salt 0.2-0.4g and pure water 80-100g；
The photocatalyst is one or both of nano-titanium dioxide 0.2-0.3g, nano zine oxide 0.2-0.3g.
2. a kind of photo-catalytic antibacterial according to claim 1 helps growing plant tissue culture medium (TCM), which is characterized in that further include
At least one of vitamin B1 and vitamin B6.
3. a kind of photo-catalytic antibacterial according to claim 1 to 2 helps growing plant tissue culture medium (TCM), which is characterized in that
By weight, comprising: ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-
1.4g, nano-titanium dioxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g,
6-BA 0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g, seven water sulphur
Sour magnesium 0.2-0.4g and pure water 80-100g.
4. a kind of photo-catalytic antibacterial according to claim 3 helps growing plant tissue culture medium (TCM), which is characterized in that by weight
Than composition, comprising: ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, vitamin B2 0.03g, epsom salt 0.3g and pure water 90g.
5. a kind of photo-catalytic antibacterial according to claim 1 helps growing plant tissue culture medium (TCM), which is characterized in that further include
Agar.
6. a kind of photo-catalytic antibacterial according to claim 4 helps the preparation method of growing plant tissue culture medium (TCM), feature
It is, comprising:
(1) 80-100ml pure water is added in container, is heated to boiling；
(2) ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, nanometer are taken
Titanium dioxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA
0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g, appearance is sequentially added
It in device, and stirs evenly, obtains mixed liquor；
(3) mixed liquor 0.2-0.4g epsom salt is adjusted into pH to 5.6-5.8, and with after conical flask packing and sealing, put
It is placed in high-pressure steam sterilizing pan the 45-60min that sterilizes, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
7. a kind of photo-catalytic antibacterial according to claim 6 helps the preparation method of growing plant tissue culture medium (TCM), feature
It is, comprising:
(1) 90ml pure water is added in container, is heated to boiling；
(2) it takes ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano-titanium dioxide 0.25g, receive
Rice zinc oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g,
Vitamin B2 0.03g is sequentially added in container, and is stirred evenly, and mixed liquor is obtained；
(3) mixed liquor for obtaining (2) 0.3g epsom salt adjusts pH to 5.7, and with after conical flask packing and sealing, puts
It is placed in high-pressure steam sterilizing pan the 50min that sterilizes, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor that sterilizing is completed is put into super-clean bench cooling.
8. a kind of -7 any photo-catalytic antibacterials help growing plant tissue culture medium (TCM) in radix tetrastigme, gold according to claim 1
Application in line lotus, rhizoma polygonati or candidum tissue culturing.
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