CN106973794A - A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof - Google Patents
A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof Download PDFInfo
- Publication number
- CN106973794A CN106973794A CN201710298062.9A CN201710298062A CN106973794A CN 106973794 A CN106973794 A CN 106973794A CN 201710298062 A CN201710298062 A CN 201710298062A CN 106973794 A CN106973794 A CN 106973794A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- vitamin
- plant tissue
- tcm
- mixed liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003104 tissue culture media Substances 0.000 title claims abstract description 63
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 36
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 35
- 230000001699 photocatalysis Effects 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 58
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 52
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910001868 water Inorganic materials 0.000 claims abstract description 38
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 32
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 30
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 30
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 29
- 239000008103 glucose Substances 0.000 claims abstract description 29
- 239000011780 sodium chloride Substances 0.000 claims abstract description 29
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 26
- 239000004471 Glycine Substances 0.000 claims abstract description 26
- 239000001110 calcium chloride Substances 0.000 claims abstract description 26
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 26
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 26
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 14
- 239000011941 photocatalyst Substances 0.000 claims abstract description 13
- 229940088594 vitamin Drugs 0.000 claims abstract description 6
- 229930003231 vitamin Natural products 0.000 claims abstract description 6
- 235000013343 vitamin Nutrition 0.000 claims abstract description 6
- 239000011782 vitamin Substances 0.000 claims abstract description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims description 47
- 229930003451 Vitamin B1 Natural products 0.000 claims description 27
- 229960003495 thiamine Drugs 0.000 claims description 27
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 27
- 235000010374 vitamin B1 Nutrition 0.000 claims description 27
- 239000011691 vitamin B1 Substances 0.000 claims description 27
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 25
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 24
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 24
- 229930003471 Vitamin B2 Natural products 0.000 claims description 24
- 229960002477 riboflavin Drugs 0.000 claims description 24
- 235000019164 vitamin B2 Nutrition 0.000 claims description 24
- 239000011716 vitamin B2 Substances 0.000 claims description 24
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims description 21
- 238000009835 boiling Methods 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 10
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 7
- 239000011777 magnesium Substances 0.000 claims description 7
- 229910052749 magnesium Inorganic materials 0.000 claims description 7
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 5
- 239000005864 Sulphur Substances 0.000 claims description 5
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 4
- 235000019158 vitamin B6 Nutrition 0.000 claims description 4
- 239000011726 vitamin B6 Substances 0.000 claims description 4
- 229940011671 vitamin b6 Drugs 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 239000004408 titanium dioxide Substances 0.000 claims description 2
- 239000011787 zinc oxide Substances 0.000 claims description 2
- 229940088710 antibiotic agent Drugs 0.000 claims 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- 240000002853 Nelumbo nucifera Species 0.000 claims 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims 1
- 239000003054 catalyst Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 40
- 241000894006 Bacteria Species 0.000 abstract description 23
- 230000012010 growth Effects 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 32
- 206010020649 Hyperkeratosis Diseases 0.000 description 12
- 239000000463 material Substances 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 230000008467 tissue growth Effects 0.000 description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 229910052791 calcium Inorganic materials 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 229930002875 chlorophyll Natural products 0.000 description 6
- 235000019804 chlorophyll Nutrition 0.000 description 6
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000006259 organic additive Substances 0.000 description 5
- 230000008635 plant growth Effects 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000005660 chlorination reaction Methods 0.000 description 4
- 239000003630 growth substance Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 235000019786 weight gain Nutrition 0.000 description 4
- 241000719837 Anoectochilus Species 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 241000026010 Dendrobium candidum Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000007146 photocatalysis Methods 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 240000004638 Dendrobium nobile Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003486 chemical etching Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000030968 tissue homeostasis Effects 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Help growing plant tissue culture medium (TCM) and preparation method thereof the invention discloses a kind of photo-catalytic antibacterial, the culture medium by weight, including the following raw material：The 1.2g of ammonium nitrate 0.8, the 0.6g of potassium dihydrogen phosphate 0.4, the 1.2g of sodium chloride 0.8, the 1.4g of glucose 1.2, the 0.5g of photocatalyst 0.1, the 0.3g of calcium chloride 0.2, the 0.06g of glycine 0.04,6 BA0.02 0.03g, IAA0.02 0.03g, the 0.08g of vitamin 0.04, the 0.4g of epsom salt 0.2 and the 100g of pure water 80.Culture provided by the present invention is based on before inoculated plant tissue, and miscellaneous bacteria can be fully killed under ultraviolet irradiation, and has promotion growth to Plant Tissue Breeding.
Description
Technical field
The invention belongs to field of tissue culture, specially a kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and its system
Preparation Method.
Background technology
Plant tissue culture media is the nutriment of the artificial preparation of plant tissue growth and maintenance, typically all contains inorganic battalion
Support thing, carbon source, vitamin, growth regulatory substance and Organic additives etc..The culture medium for cultivating different plant cell tissues can root
According to being actually needed, the necessary element of some plant cell growths is added.
Existing plant tissue culture media is after completing component preparation and loading narrow-mouthed bottle or culture dish, usually using cloth
Material or paper seal narrow-mouthed bottle bottleneck or the interface using ParafilmTM culture dish, and narrow-mouthed bottle then is put into high steam
Sterilization treatment 45-60min in autoclave, or after culture dish is lived with paper bag, it is put into sterilization treatment 45- in high-pressure steam sterilizing pan
60min.After the completion of high pressure steam sterilization, narrow-mouthed bottle is transferred in super-clean bench and cools down, be inoculated with.
But above-mentioned plant tissue culture media sterilizing after the completion of be transferred to super-clean bench during, culture medium easily by
Living contaminants.
Also, one layer of bacterium on culture dish surface can only be killed using ultraviolet on super-clean bench, it is impossible to thoroughly to culture
Whole culture mediums in ware are thoroughly sterilized.When culture medium is contained using narrow-mouthed bottle, ultraviolet light can not be to middle in bottle
The culture medium at position is sterilized, the plant tissue for causing living contaminants to be cultivated, and is unfavorable for plant tissue growth situation
Observation, miscellaneous bacteria also sucks the nutrient in culture medium, and inhibitory action is played in the growth to plant tissue.
The content of the invention
Growing plant tissue culture medium (TCM) is helped the invention provides a kind of photo-catalytic antibacterial, the photo-catalytic antibacterial helps growing plant
Tissue culture medium (TCM) solves existing culture medium and sterilized by ultraviolet irradiation, it is impossible to which the miscellaneous bacteria for killing culture medium middle part is asked
Topic.
To achieve the above object, the present invention provides following technical scheme:
A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM), by weight, including following composition：Ammonium nitrate 0.8-
1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, photocatalyst 0.1-0.5g, calcium chloride
0.2-0.3g, glycine 0.04-0.06g, 6-BA0.02-0.03g, IAA0.02-0.03g, vitamin 0.04-0.08, seven water sulphur
Sour magnesium 0.2-0.4g and pure water 80-100g.
Wherein, the inorganic nutrients thing needed in plant tissue culture course is contained in culture medium：Epsom salt, chlorination
Calcium, ammonium nitrate, potassium dihydrogen phosphate, sodium chloride；Carbon source：Glucose；Organic additives：Glycine and vitamin；Growth regulator：
IAA and 6-BA.Above-mentioned five major classes nutriment is indispensable during plant tissue growth.
Meanwhile, 0.8-1.2g ammonium nitrate provides the nitrogen source of abundance for plant tissue growth, can effectively facilitate plant thin
The generation of protein, chlorophyll and nucleic acid in born of the same parents；0.4-0.6g potassium dihydrogen phosphates are also biomembrane, nucleotides in plant tissue
And the formation of nucleic acid is there is provided sufficient P elements, and the potassium element that potassium dihydrogen phosphate is provided can promote nitrogen metabolism
And the transfer of carbohydrate, so as to accelerate the growth of plant tissue；0.2-0.3g calcium chloride is plant tissue cell's wall
Growth there is provided sufficient calcium constituent；In addition, the culture medium also added epsom salt as magnesium source, accelerate chlorophyll
Formed；The Organic additives and growth regulator of addition can promote plant tissue fast-growth in culture medium.
Also, photocatalyst is also added with the culture medium, photocatalyst will not only suppress the growth of plant cell, Er Qieke
The microorganism infected during being transferred to super-clean bench from high-pressure steam sterilizing pan with effectively kill, compensate for the purple in super-clean bench
Outer bactericidal assay can not kill the defect of miscellaneous bacteria inside culture medium.Miscellaneous bacteria is avoided to grow the life of interference experiment personal observations' plant tissue
Long or miscellaneous bacteria, which absorbs the excessive nutrient of culture medium, causes the generation for the problems such as plant tissue growth is affected；
When culture medium be placed on open ultraviolet light super-clean bench under irradiation-sterilize when, photocatalyst can make full use of oxygen and
Water, carbon dioxide and water are converted into by the miscellaneous bacteria in culture medium, so as to reach the effect of sterilization；In addition, photocatalyst can also be improved
Plant tissue photocatalysis efficiency, promotes the plant tissue for having formed chlorophyll to carry out photosynthesis.
As further preferred, the bactericidal agent is one or both of nano titanium oxide, nano zine oxide.Nanometer
Titanium dioxide and nano zine oxide not only have a very high photocatalytic activity, and with acid-alkali-corrosive-resisting, resistant to chemical etching, nontoxic
The advantages of, price is also moderate, with higher cost performance.
Preferably, the antibacterial helps growing plant tissue culture medium (TCM) also to include：At least one in vitamin B1, vitamin B6
Kind.Growth of the vitamin B6 to root has facilitation, and the generation of vitamin B1 and callus and viability have substantial connection.Cause
This, addition vitamin B1 and vitamin B6 can effectively facilitate the growth of plant tissue in culture medium.
Preferably, by weight, the antibacterial helps growing plant tissue culture medium (TCM) to include：Ammonium nitrate 0.8-1.2g, phosphorus
It is acid dihydride potassium 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, nano titanium oxide 0.2-0.3g, nano oxidized
Zinc 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA 0.02-0.03g, IAA 0.02-0.03g, dimension
Raw element B1 0.02-0.04g, vitamin B2 0.02-0.04g, epsom salt 0.2-0.4g and pure water 80-100g.It is real
Checking is bright, takes that the product bactericidal effect obtained by the weight percentage is more notable, and the growth result of plant tissue is notable
Lifting.
As further preferred, by weight, the antibacterial helps growing plant tissue culture medium (TCM) to include：Ammonium nitrate 1.0g,
Potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide 0.25g, nano zine oxide 0.25g, calcium chloride
0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven water sulphur
Sour magnesium 0.3g and pure water 90g.
Preferably, the antibacterial helps growing plant tissue culture medium (TCM) also to include agar.Experimenter can be according to plant group
Knit and choose whether to add agar in different growth ring sections.
Present invention also offers the preparation method that the antibacterial helps growing plant tissue culture medium (TCM), the preparation method includes：
(1) 80-100g pure water is added in container, boiling is heated to；
(2) take ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g,
Nano titanium oxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA
0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g sequentially add container
It is interior, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into pH to 5.7 with 0.2-0.4g epsom salts, and is dispensed and after sealing, put with conical flask
Sterilizing 45-60min in high-pressure steam sterilizing pan is placed in, the mixed liquor that sterilizing is completed is obtained；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Wherein, during (2) step prepares mixed liquor, according to above-mentioned order, after a kind of medicine fully dissolves again
Add another medicine.It is uniform using culture medium Nutrient distribution made from above-mentioned steps, and culture medium passes through high pressure steam sterilization
Pot sterilizing, it is to avoid miscellaneous bacteria produces interference to the plant cell growth of required culture.Even if culture medium is from high pressure steam sterilization
Pot infects the photocatalyst contained in miscellaneous bacteria, culture medium under ultraviolet irradiation during being transferred to super-clean bench, it is possible to use
Miscellaneous bacteria is resolved into carbon dioxide and water by oxygen and moisture in culture medium, reaches degerming effect.
As further preferred, the preparation method is specially：
(1) 90g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide are taken
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, vitamin B2 0.03g are sequentially added in container, and stirred, and obtain mixed liquor；
(3) mixed liquor is adjusted into pH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 50min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Growing plant tissue culture medium (TCM) is helped in radix tetrastigme, roxburgh anoectochilus terminal bud, sealwort or iron sheet present invention also offers above-mentioned antibacterial
Application in the tissue cultures such as the stem of noble dendrobium.
Compared with prior art, beneficial effects of the present invention are：
(1) the inorganic nutrients thing needed in plant tissue culture course is contained in culture medium provided by the present invention：Seven water
Magnesium sulfate, calcium chloride, ammonium nitrate, potassium dihydrogen phosphate, sodium chloride；Carbon source：Glucose；Organic additives：Glycine and vitamin；
Growth regulator：IAA and 6-BA.Above-mentioned five major classes nutriment is indispensable during plant tissue growth；
Meanwhile, 0.8-1.2g ammonium nitrate provides the nitrogen source of abundance for plant tissue growth, can effectively facilitate plant thin
The generation of protein, chlorophyll and nucleic acid in born of the same parents；0.4-0.6g potassium dihydrogen phosphates are also biomembrane, nucleotides in plant tissue
And the formation of nucleic acid is there is provided sufficient P elements, and the potassium element that potassium dihydrogen phosphate is provided can promote nitrogen metabolism
And the transfer of carbohydrate, so as to accelerate the growth of plant tissue；0.2-0.3g calcium chloride is plant tissue cell's wall
Growth there is provided sufficient calcium constituent；In addition, the culture medium also added epsom salt as magnesium source, accelerate chlorophyll
Formed；The Organic additives and growth regulator of addition can promote plant tissue fast-growth in culture medium.
(2) photocatalyst is also added with culture medium provided by the present invention, photocatalyst will not only suppress plant cell
Growth, and can effectively kill be transferred to super-clean bench from high-pressure steam sterilizing pan during the microorganism that is infected, compensate for
Ultraviolet-sterilization method in super-clean bench can not kill the defect of miscellaneous bacteria inside culture medium.Miscellaneous bacteria is avoided to grow interference experiment personal observations
The growth of plant tissue or miscellaneous bacteria, which absorb the excessive nutrient of culture medium, causes the generation for the problems such as plant tissue growth is affected.
Embodiment
Technical scheme is described in further detail using embodiment below.
Embodiment 1
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, nano titanium oxide 0.2g, chlorination
Calcium 0.2g, glycine 0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, seven water sulphur
Sour magnesium 0.2g and pure water 80g.
Its preparation method includes：
(1) 80g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, nano titanium oxide are taken
0.2g, calcium chloride 0.2g, glycine 0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2
0.02g, sequentially add in container, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into pH to 5.7 with 0.2g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 45min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Comparative example 1
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, calcium chloride 0.2g, glycine
0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, epsom salt 0.2g and pure
Water purification 80g.
Its preparation method includes：
(1) 80g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, calcium chloride 0.2g, sweet ammonia are taken
Sour 0.04g, 6-BA 0.02g, IAA 0.02g, vitamin B1 0.02g, vitamin B2 0.02g, sequentially add in container, and
Stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.2g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 45min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Embodiment 2
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide 0.25g, chlorine
Change calcium 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven
Water magnesium sulfate 0.3g and pure water 90g.
Its preparation method includes：
(1) 90g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide are taken
0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2
0.03g, sequentially add in container, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Comparative example 2
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, calcium chloride 0.25g, glycine
0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, epsom salt 0.3g and
Pure water 90g.
Its preparation method includes：
(1) 90g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, calcium chloride 0.25g, sweet ammonia are taken
Sour 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, sequentially add in container,
And stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Embodiment 3
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, nano titanium oxide 0.3g, chlorination
Calcium 0.3g, glycine 0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, seven water sulphur
Sour magnesium 0.4g and pure water 100g.
Its preparation method includes：
(1) 100g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, nano titanium oxide are taken
0.3g, calcium chloride 0.3g, glycine 0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2
0.04g, sequentially add in container, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.4g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 60min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Comparative example 3
By weight, the present embodiment provides a kind of photo-catalytic antibacterial and helps growing plant tissue culture medium (TCM), by following material group
Into：
Ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, calcium chloride 0.3g, glycine
0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, epsom salt 0.4g and pure
Water purification 100g.
Its preparation method includes：
(1) 100g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, calcium chloride 0.3g, sweet ammonia are taken
Sour 0.06g, 6-BA 0.03g, IAA 0.03g, vitamin B1 0.04g, vitamin B2 0.04g, sequentially add in container, and
Stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.4g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 60min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Embodiment 4
By weight, the present embodiment provides a kind of antibacterial and helps growing plant tissue culture medium (TCM), by following material composition：
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide 0.25g, receive
Rice zinc oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g,
Vitamin B2 0.03g, epsom salt 0.3g and pure water 90g.
Its preparation method includes：
(1) 90g pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide are taken
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, vitamin B2 0.03g, sequentially add in container, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Embodiment 5
By weight, the present embodiment provides a kind of antibacterial and helps growing plant tissue culture medium (TCM), by following material composition：
Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano zine oxide 0.25g, chlorination
Calcium 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g, seven water
Magnesium sulfate 0.3g and pure water 90g.
Its preparation method includes：
(1) 90g pure water is added in container, boiling is heated to；
(2) take ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano zine oxide 0.25g,
Calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g, vitamin B2 0.03g,
Sequentially add in container, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into PH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, be positioned over conical flask
Sterilizing 55min in high-pressure steam sterilizing pan, obtains the mixed liquor that sterilizing is completed；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
Comparative example 4
MS culture mediums, preparation method and embodiment 2 are identical.
Comparative example 5
B5 medium, preparation method and embodiment 2 are identical.
Comparative example 6
N6 culture mediums, preparation method and embodiment 2 are identical.
Test case 1
The present invention is applicable the Plant Tissue Breeding of radix tetrastigme, roxburgh anoectochilus terminal bud, sealwort or dendrobium candidum etc..Now it is cured with cultivating sealwort
Exemplified by injured tissue.
It is 1,2,3,4,5,6,7,8,9 that embodiment 1-3 and comparative example the 1-6 culture medium provided are numbered respectively, will be real
It is 10 to apply the numbering of example 4, is 11 by the numbering of embodiment 5.The sealwort callus of Zhejiang A ＆ F University's Experimental Base plantation will be derived from
It is seeded to respectively in 1-11 groups, 20 parallel laboratory test groups of every group of setting；Meanwhile, 1-11 groups are correspondingly arranged blank control group respectively,
Sealwort callus it is not inoculated with, numbering is A-K, 20 parallel laboratory test groups of every group of setting.
1-11 groups and A-K groups are cultivated 20 days under 26 ± 1 DEG C of poor light conditions after inoculation, more each culture medium Neihuang County is observed
The growing state statistics such as following table of smart callus：
The sealwort callus growth situation statistical form of table 1
Bacterium colony generates situation in the blank control group A-I group culture mediums of table 2
The rate of body weight gain of the 1-3 groups of table 1 is all more than 84%, hence it is evident that higher than the 4-6 groups of table 1, illustrates that the present invention is added
Growth of the photocatalyst to sealwort callus has obvious facilitation effect, and its reason is due to that photocatalyst promotes to have formed leaf
The photocatalysis efficiency of green plain sealwort callus, so as to accelerate it to grow；
The reason for 10th group of sealwort callus rate of body weight gain is higher than 1-3 group sealwort callus is：Tenth tissue culture
The combination using nano titanium oxide and nano zine oxide in base is supported, the effect of Synergistic is served, promotes plant cell
Photosynthesis；
And the not high another reason of 4-6 groups rate of body weight gain is：4-6 groups cause with chlorophyll without addition photocatalyst
Plant cell photosynthesis rate it is slow, it is impossible to produce enough nutrients, thus weightening is slow；By the weightening of the 1-3 groups of table 1
Rate understands that instant component is more suitable for compared to MS culture mediums, B5 medium, N6 culture mediums higher than the rate of body weight gain of 7-9 groups
Sealwort callus growth, and each component has synergistic function, promotes sealwort callus rapid growth.
A-C and D-I groups are compared in table 2, and the quantity of the culture medium of A-C groups generation bacterium colony is significantly lower than D-I groups,
It can draw：The nano titanium oxide added in the present invention in culture medium has a good bactericidal effect, and embodiment 2 is killed
Bacterium best results, while in the 10th group of blank control group J groups, the culture medium number for having bacterium colony to generate is 0, hence it is evident that less than reality
Example 2, i.e. B groups are applied, its reason is：Using the combination of nano titanium oxide and nano zine oxide in J group culture mediums, play
The effect of Synergistic so that the culture medium bactericidal effect greatly increases.
The quantity of culture medium of the quantity more than A-C groups generation bacterium of the culture medium of bacterium is generated by K groups and is given birth to by K groups
Quantity into the culture medium of bacterium is less than the quantity that D-I groups generate the culture medium of bacterium, can learn：In the culture medium of the present invention
The nano zine oxide of middle addition has preferable bactericidal effect, but bactericidal effect does not have nano titanium oxide and nano-silica
Change titanium, nano zine oxide to combine；
Shown according to the result of test case 1：Culture medium provided by the present invention is before inoculated plant tissue, in ultraviolet irradiation
Under can fully kill miscellaneous bacteria, and have to the culture of sealwort callus and promote growth.
In the same manner, the present invention is also suitable the culture of the plant tissues such as radix tetrastigme, roxburgh anoectochilus terminal bud or dendrobium candidum.
Claims (9)
1. a kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM), it is characterised in that by weight, including following component：Nitre
Sour ammonium 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, photocatalyst 0.1-0.5g,
Calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA0.02-0.03g, IAA0.02-0.03g, vitamin 0.04-
0.08g, epsom salt 0.2-0.4g and pure water 80-100g.
2. a kind of photo-catalytic antibacterial according to claim 1 helps growing plant tissue culture medium (TCM), it is characterised in that the light
Catalyst is one or both of nano titanium oxide, nano zine oxide.
3. a kind of photo-catalytic antibacterial according to claim 1 helps growing plant tissue culture medium (TCM), it is characterised in that also include
At least one of vitamin B1 and vitamin B6.
4. growing plant tissue culture medium (TCM) is helped according to a kind of any described photo-catalytic antibacterials of claim 1-3, it is characterised in that
By weight, including：Ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-
1.4g, nano titanium oxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g,
6-BA 0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g, seven water sulphur
Sour magnesium 0.2-0.4g and pure water 80-100g.
5. a kind of photo-catalytic antibacterial according to claim 4 helps growing plant tissue culture medium (TCM), it is characterised in that by weight
Than composition, including：Ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide
0.25g, nano zine oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1
0.03g, vitamin B2 0.03g, epsom salt 0.3g and pure water 90g.
6. a kind of photo-catalytic antibacterial according to claim 1 helps growing plant tissue culture medium (TCM), it is characterised in that also include
Agar.
7. a kind of photo-catalytic antibacterial according to claim 5 helps the preparation method of growing plant tissue culture medium (TCM), its feature
It is, including：
(1) 80-100ml pure water is added in container, boiling is heated to；
(2) ammonium nitrate 0.8-1.2g, potassium dihydrogen phosphate 0.4-0.6g, sodium chloride 0.8-1.2g, glucose 1.2-1.4g, nanometer are taken
Titanium dioxide 0.2-0.3g, nano zine oxide 0.2-0.3g, calcium chloride 0.2-0.3g, glycine 0.04-0.06g, 6-BA
0.02-0.03g, IAA 0.02-0.03g, vitamin B1 0.02-0.04g, vitamin B2 0.02-0.04g, sequentially add appearance
In device, and stir, obtain mixed liquor；
(3) mixed liquor is adjusted into pH to 5.6-5.8 with 0.2-0.4g epsom salts, and is dispensed and after sealing, put with conical flask
Sterilizing 45-60min in high-pressure steam sterilizing pan is placed in, the mixed liquor that sterilizing is completed is obtained；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
8. a kind of photo-catalytic antibacterial according to claim 7 helps the preparation method of growing plant tissue culture medium (TCM), its feature
It is, including：
(1) 90ml pure water is added in container, boiling is heated to；
(2) ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g are taken, sodium chloride 1.0g, glucose 1.3g, nano titanium oxide 0.25g, is received
Rice zinc oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, 6-BA 0.025g, IAA 0.025g, vitamin B1 0.03g,
Vitamin B2 0.03g is sequentially added in container, and is stirred, and obtains mixed liquor；
(3) mixed liquor for obtaining (2) adjusts pH to 5.7 with 0.3g epsom salts, and is dispensed and after sealing, put with conical flask
Sterilizing 50min in high-pressure steam sterilizing pan is placed in, the mixed liquor that sterilizing is completed is obtained；
(4) mixed liquor for the completion that sterilizes is put into super-clean bench cooling.
9. growing plant tissue culture medium (TCM) is helped in radix tetrastigme, gold according to a kind of any described photo-catalytic antibacterials of claim 1-8
Application in line lotus, sealwort or candidum tissue culturing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298062.9A CN106973794B (en) | 2017-04-29 | 2017-04-29 | A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298062.9A CN106973794B (en) | 2017-04-29 | 2017-04-29 | A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106973794A true CN106973794A (en) | 2017-07-25 |
| CN106973794B CN106973794B (en) | 2019-03-12 |
Family
ID=59343268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710298062.9A Active CN106973794B (en) | 2017-04-29 | 2017-04-29 | A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106973794B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107266236A (en) * | 2017-08-18 | 2017-10-20 | 张悦健 | A kind of soilless culture plantation nutrient solution and preparation method thereof |
| CN107324920A (en) * | 2017-08-18 | 2017-11-07 | 张悦健 | A kind of vegetable soilless-culture nutrient solution and preparation method thereof |
| CN107473819A (en) * | 2017-08-18 | 2017-12-15 | 张悦健 | A kind of water culture nutrient solution and preparation method thereof |
| CN112237140A (en) * | 2020-10-16 | 2021-01-19 | 云南省农业科学院花卉研究所 | Method for blocking dendrobium officinale tissue culture seedling from being polluted by bacteria |
| CN112280689A (en) * | 2020-10-18 | 2021-01-29 | 绿城农科检测技术有限公司 | Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof |
-
2017
- 2017-04-29 CN CN201710298062.9A patent/CN106973794B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107266236A (en) * | 2017-08-18 | 2017-10-20 | 张悦健 | A kind of soilless culture plantation nutrient solution and preparation method thereof |
| CN107324920A (en) * | 2017-08-18 | 2017-11-07 | 张悦健 | A kind of vegetable soilless-culture nutrient solution and preparation method thereof |
| CN107473819A (en) * | 2017-08-18 | 2017-12-15 | 张悦健 | A kind of water culture nutrient solution and preparation method thereof |
| CN112237140A (en) * | 2020-10-16 | 2021-01-19 | 云南省农业科学院花卉研究所 | Method for blocking dendrobium officinale tissue culture seedling from being polluted by bacteria |
| CN112280689A (en) * | 2020-10-18 | 2021-01-29 | 绿城农科检测技术有限公司 | Culture medium capable of promoting rapid growth of osmophilic yeast in honey and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106973794B (en) | 2019-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106973794B (en) | A kind of photo-catalytic antibacterial helps growing plant tissue culture medium (TCM) and preparation method thereof | |
| CN102835251B (en) | Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid | |
| CN107162652A (en) | A kind of odorless feces of livestock and poultry organic fertilizer and its preparation method and application | |
| CN106914128A (en) | A kind of biological deodorant and preparation method thereof | |
| CN106509081B (en) | A kind of probiotics edible mushroom mixed fermentation fruits and vegetables bio-preservative and preparation method thereof | |
| WO2017045274A1 (en) | Nitrogen-free medium and method for identifying whether free-living nitrogen-fixing bacteria secrete ammonia by using same | |
| CN108046858A (en) | A kind of method using sick dead pig hamlessizing technology organic fertilizer | |
| CN101518356B (en) | Method for sterilizing pollen | |
| CN106171970B (en) | Rapid preparation method of plant culture medium with low phenol content | |
| CN107021828A (en) | A kind of Jujun grasses azotobacteria fertilizer and preparation method thereof | |
| CN106635919A (en) | Spirulina culture method | |
| CN106676055B (en) | A method of reducing the pollution of plant tissue culture media surface | |
| CN105886430A (en) | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation | |
| CN105925482A (en) | Culture medium capable of improving biological stuffing microbial activity and preparation method thereof | |
| CN105831162A (en) | Sprouting vegetable seed soaking fungicide and application thereof | |
| CN107125133B (en) | A kind of photocatalyst culture medium and preparation method thereof that rhizoma polygonati explant survival rate can be improved | |
| CN108179130A (en) | A kind of preparation method of high activity Enterococcus faecalis microorganisms preparation dry powder | |
| CN115181686A (en) | Bacillus subtilis culture preparation process not easy to be infected by mixed bacteria | |
| CN104628434B (en) | A kind of preparation method of the bio-fertilizer of anti-black fruit fructus lycii pathology fungi | |
| CN109133354B (en) | Algae-based ecological bacteriostasis method for wastewater treatment | |
| CN107487853A (en) | One kind is used for aquaculture water transfer microorganism formulation | |
| CN103202412A (en) | Biological preservation processing and producing method for rice bran | |
| CN106333059A (en) | Mixed fermentation method of biological feed leavening agent | |
| CN110305812A (en) | A kind of the lichen bacillus ferments culture process | |
| CN108432547B (en) | Synthetic culture method for mycorrhiza |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240508 Address after: 1217, 3rd Floor, Building C2, No.1 Huangchang West Road, Dougezhuang, Chaoyang District, Beijing, 100020 Patentee after: Yinian Caomu (Beijing) Tea Industry Co.,Ltd. Country or region after: China Address before: No. 88, Ling'an City, Zhejiang, Hangzhou, Zhejiang Patentee before: ZHEJIANG A & F University Country or region before: China |
|
| TR01 | Transfer of patent right |