CN105432181B - The method for promoting gomuti palm seed to sprout - Google Patents
The method for promoting gomuti palm seed to sprout Download PDFInfo
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- CN105432181B CN105432181B CN201510923276.1A CN201510923276A CN105432181B CN 105432181 B CN105432181 B CN 105432181B CN 201510923276 A CN201510923276 A CN 201510923276A CN 105432181 B CN105432181 B CN 105432181B
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- gomuti palm
- palm seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a kind of method for promoting gomuti palm seed to sprout, comprise the following steps：Step 1: it is 1 by volume by gomuti palm seed and wet perlite:3 is well mixed, after sealing, lamination 90 days at 10 15 DEG C, obtains the gomuti palm seed after lamination；Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, it is placed in the drier equipped with dry silica gel, drying at room temperature 3h, obtains dry gomuti palm seed.The present invention has the advantages that simple to operate, cost is low, easy to spread and can improve percentage of seedgermination.
Description
Technical field
The present invention relates to a kind of method for promoting seed to sprout.It is more particularly related to a kind of promote gomuti palm kind
The method that son is sprouted.
Background technology
Gomuti palm is Palmae Arenga arbor, inflorescence juice can granulated sugar processed, the entitled gomuti palm powder of starch of medulla extraction, have
The features such as without fat, low calorie, high microsteping, and containing a variety of the essential trace elements of the human bodys such as copper, iron, zinc, there is remuval of damp and hot and taste
The function of benefit, there is the effect of auxiliary treatment to infantile malnutrition, heating, dysentery, laryngopharyngitis etc., be the treasure in Forest Nutrition food
Product.In addition, gomuti palm is a kind of multiduty nonwood forest trees in Southwestern China area, it is a kind of excellent afforestation of stony desertification tor again
Green tree species.
Gomuti palm is wild in Mountainous Area, slow-growing, plant cut after again without sprouting ability again.In recent years, gomuti palm tree in blocks has been
Rarely found, some places are almost endangered.Have no that its vegetative propagation is successfully reported, bred in production with seed.
Gomuti palm seed ovate prismatic, mass of 1000 kernel 2955.8g.In test we have found that mainly existing the reason for gomuti palm seed dormancy
Germination inhibitor, belong to physiological dormancy.Undressed seed sprouts cycle length, and germination rate is very low, and the germination rate of 150 days is
35%.The difficult sprouting of seed, which has become, restricts its serious problems further effectively utilized.
At present, the method for physiological seed dormancy being released in production is mainly that lamination, seed soaking or gibberellin are handled, land ancestral
Positive to wait the research to gomuti palm seed to show, the germination rate of 150 days is 48% and 34% after 80 DEG C of warm water and gibberellin processing, is failed
Significantly improve the sprout time and germination rate of seed.Therefore, a kind of more effective method for improving gomuti palm seed and sprouting is needed badly.
The content of the invention
It is an object of the invention to provide a kind of method for promoting gomuti palm seed to sprout, this method has simple to operate, cost
It is low, easy to spread and the advantages that percentage of seedgermination can be improved.
In order to realize according to object of the present invention and further advantage, there is provided a kind of side for promoting gomuti palm seed to sprout
Method, comprise the following steps：
Step 1: it is 1 by volume by gomuti palm seed and wet perlite:3 is well mixed, after sealing, in 10-15 DEG C of lower floor
Product 90 days, obtains the gomuti palm seed after lamination；
Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, the drying equipped with dry silica gel is placed in
In device, drying at room temperature 3h, dry gomuti palm seed is obtained.
Preferably, in the described method for promoting gomuti palm seed to sprout, the wet perlite by 1 parts by weight perlite
It is made with after the sterilized water mixing of 1.4-2 parts by weight.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, the gomuti palm described in the drier after lamination
The volume ratio of seed and the dry silica gel is 1:100.
Preferably, in the described method for promoting gomuti palm seed to sprout, in the step 1 by the gomuti palm seed with
Before the wet perlite mixing, in addition to the step of sterilized to the gomuti palm seed, it is concretely comprised the following steps：
The gomuti palm seed is put into after soaking 5min in the liquor potassic permanganate that mass fraction is 0.1%, taken out, with nothing
Bacterium water rinses 2-3 times.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, in addition to：
After being sterilized in the step 1 to the gomuti palm seed, the gomuti palm seed and the wet perlite are mixed
Before conjunction, the gas-phase silica that volume is the wet perlite volume 20-30% is added into the wet perlite, wherein, one
Half gas-phase silica absorbs the 200-300 times of liquid of 50% carbendazol wettable powder for having 10-20 times of deadweight, second half
The gas-phase silica absorbs the 1000-1500 times of liquid of 2.5% pleocidin suspension for having 3-5 times of deadweight.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, in addition to：
Step 3: being sterilized to dry gomuti palm seed, it is concretely comprised the following steps：
First dry gomuti palm seed is put into the ethanol water that volume fraction is 75% and soaks 0.5min, is taken out, then
It is put into the aqueous sodium hypochlorite solution that mass fraction is 1% and soaks 5min, take out, with aseptic water washing 3 times, after is sterilized
Dry gomuti palm seed.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, in addition to：
Step 4: sprouting culture is carried out to the gomuti palm seed of the drying after the sterilization that is obtained in step 3, its specific steps
For：
After equably laying depth is 1-2cm perlite in a diameter of 13cm culture dish, added into culture dish
100-110mL sterilized waters, then the gomuti palm seed of the drying after 50 sterilizations is placed in culture dish, mixed with perlite
It is even, culture dish is sealed with sealed membrane afterwards, at 30 DEG C, 12h is first cultivated under illumination condition daily, then under dark condition
Cultivate 12h, continuous culture 90 days.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, in step 4, in addition to：
It is 0.8-1cm to the bottom even laying depth of the culture dish before laying perlite in the culture dish
Medical stone ball, and 10-15g 100-150 times of liquid of 50% carbendazol wettable powder is poured into culture dish, afterwards described
One layer and culture dish diameter identical filter paper are laid on medical stone ball, perlite is laid on the filter paper；
After the gomuti palm seed of drying after sterilization is placed in culture dish, before being well mixed with perlite, to pearl
In rock add 10-20g absorptions have deadweight 200-300 times of bactericide water-loss reducer, afterwards with perlite and sterilize after drying
Gomuti palm seed be well mixed, 5-10g glycerine is uniformly sprayed on the surface of most backward perlite, wherein, the bactericide by with
The component of lower parts by weight is made：Lysozyme 0.2-0.3 parts, lywallzyme 0.3-0.5 parts, chitosan 0.1-0.2 parts, pyrolkigneous liquid 1-
3 parts and water 20-30 parts.
Preferably, in the described method for promoting the sprouting of gomuti palm seed, the bottom of the culture dish is coated with photocatalyst,
The described method for promoting the sprouting of gomuti palm seed, in addition to：
After with sealed membrane, the culture dish is sealed, when being cultivated daily under illumination condition, the training described in ultraviolet light
Support the bottom 3-5min of ware, Continuous irradiation 90 days.
The present invention comprises at least following beneficial effect：
The present invention was by the Stratificated treatment of 90 days and gomuti palm seed is placed in drier before sprouting processing is dried,
Sprouting and the seedling of gomuti palm seed can be effectively facilitated, improves the germination rate of gomuti palm seed.
The present invention have the advantages that cost it is low, without it is dangerous, be easy for workers to operate, pollution-free, easy to spread, to the kind of gomuti palm
Sub- nursery, large-scale planting are of very high actual application value.
The present invention is before lamination, and being added into wet perlite has the white black wood charcoal of gas phase, and it is adsorbed with the carbendazim that can be sterilized can
WP and can desinsection more rhzomorph suspending agents, gas-phase silica have the more meso-hole structures of huge specific surface area, surface and
Superpower adsorption capacity and unusual physicochemical property, more carbendazim, more rhzomorphs and moisture etc. can be adsorbed, and in lamination mistake
Slowly discharged in journey, make gomuti palm seed be in it is sterile, without in worm and the suitable environment of humidity, improve the sprouting of gomuti palm seed
Rate.
The present invention is first equipped with medical stone ball in culture dish, and the higher more bacterium of concentration have been poured into medical stone ball
Spirit, medical stone ball have great adsorptivity, energy adsorption moisture and medicament, also have certain adsorption capacity to bacterium, and can incite somebody to action
Water is activated, a large amount of mineral elements of dissolution, is advantageous to seed sprouting, and can prevent that culture dish bottom is mouldy.
The present invention adds in culture dish the water-loss reducer for absorbing bactericide, and it can slowly release bactericide and water
Come, gomuti palm seed is in sterile growing environment, improve germination rate.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1
The present invention provides a kind of method for promoting gomuti palm seed to sprout, and comprises the following steps：
Step 1: it is 1 by volume by gomuti palm seed and wet perlite:3 is well mixed, after sealing, the lamination at 10 DEG C
90 days, obtain the gomuti palm seed after lamination；Stratificated treatment can release seed dormancy, promote seed to sprout.
Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, the drying equipped with dry silica gel is placed in
In device, drying at room temperature 3h, dry gomuti palm seed is obtained.Before culture is sprouted, some dewater treatments, Ke Yishi are carried out to seed
When offer germination rate.
In the described method for promoting gomuti palm seed to sprout, the wet perlite by 1 parts by weight perlite and 1.4 weight
It is made after the sterilized water mixing of part.
In the described method for promoting gomuti palm seed to sprout, gomuti palm seed described in the drier after lamination with it is described
The volume ratio of dry silica gel is 1:100.
Described promotes in the method that gomuti palm seed is sprouted, by the gomuti palm seed and the wet pearl in the step 1
Before rock mixing, in addition to the step of sterilized to the gomuti palm seed, it is concretely comprised the following steps：
The gomuti palm seed is put into after soaking 5min in the liquor potassic permanganate that mass fraction is 0.1%, taken out, with nothing
Bacterium water rinses 2 times.The gomuti palm seed of collection is sterilized, the germ carried on seed can be reduced or eliminated.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
After being sterilized in the step 1 to the gomuti palm seed, the gomuti palm seed and the wet perlite are mixed
Before conjunction, the gas-phase silica that volume is the wet perlite volume 20% is added into the wet perlite, wherein, half
The gas-phase silica absorbs 50% carbendazol wettable powder, the 200 times of liquid for having 10 times of deadweight, and second half the gas phase is white
Carbon black absorbs 2.5% pleocidin suspension, the 1000 times of liquid for having 3 times of deadweight.Gas-phase silica can be by 50% carbendazim of absorption
1000 times of liquid of 200 times of liquid of wettable powder and 2.5% pleocidin suspension slowly release, and can prevent higher concentration
The influence that medicament is sprouted to seed, and can is sprouted for seed longer provides suitable growing environment.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 3: being sterilized to dry gomuti palm seed, it is concretely comprised the following steps：
First dry gomuti palm seed is put into the ethanol water that volume fraction is 75% and soaks 0.5min, is taken out, then
It is put into the aqueous sodium hypochlorite solution that mass fraction is 1% and soaks 5min, take out, with aseptic water washing 3 times, after is sterilized
Dry gomuti palm seed.Dry gomuti palm seed is sterilized, prevents dry gomuti palm seed from carrying more bacterium, causes to sprout
The mold of seed in incubation is sent out, reduces germination percentage.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 4: sprouting culture is carried out to the gomuti palm seed of the drying after the sterilization that is obtained in step 3, its specific steps
For：
After equably laying depth is 1cm perlite in a diameter of 13cm culture dish, added into culture dish
100mL sterilized waters, then the gomuti palm seed of the drying after 50 sterilizations is placed in culture dish, it is well mixed with perlite,
Culture dish is sealed with sealed membrane afterwards, at 30 DEG C, 12h is first cultivated under illumination condition daily, then trained under dark condition
Support 12h, continuous culture 90 days.
Described promotes in the method that gomuti palm seed is sprouted, in step 4, in addition to：
It is 0.8cm's to the bottom even laying depth of the culture dish before laying perlite in the culture dish
Medical stone ball, and 10g 50% carbendazol wettable powder, 100 times of liquid are poured into culture dish, afterwards in the medical stone ball
Upper one layer of laying is laid on the filter paper with culture dish diameter identical filter paper, perlite；Sprout in incubation, moisture meeting
Gradually to the bottom lamination of culture dish, cause culture dish bottom easily mouldy, medical stone ball, medical stone ball are set in culture dish bottom
Bacterium can be adsorbed, and 50% carbendazol wettable powder, 100 times of liquid have been poured into culture dish, medical stone ball can absorb moisture and
50% 100 times of carbendazol wettable powder liquid, and the medicament of absorption is slowly released, it can guarantee that the bottom of culture dish is in
Germ-free condition.
After the gomuti palm seed of drying after sterilization is placed in culture dish, before being well mixed with perlite, to pearl
In rock add 10g absorptions have deadweight 200 times of bactericide water-loss reducer, afterwards with perlite and sterilize after drying gomuti palm kind
Son is well mixed, and 5g glycerine is uniformly sprayed on the surface of most backward perlite, wherein, the bactericide is by following parts by weight
Component is made：20 parts of 0.2 part of lysozyme, 0.3 part of lywallzyme, 0.1 part of chitosan, 1 part of pyrolkigneous liquid and water.Lysozyme can make bacterium
Cell wall rupture, can also with negatively charged virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make disease
Poison inactivation.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and the temperature for sprouting culture is 30 DEG C, thus
Lywallzyme can be made to be in suitable hydrolysis temperature.Chitosan has bacteriostatic activity.Pyrolkigneous liquid can suppress the breeding of miscellaneous bacteria, have
Fungi-proofing, the effect of insect prevention, acetic acid content is higher in pyrolkigneous liquid, and solution can dissolve chitosan, and make lysozyme and molten wall in acidity
Enzyme is in suitable sour environment, improves enzymolysis efficiency.It is suitable wet that glycerine can guarantee that the seed on culture dish top is in
Under degree.
Described promotes in the method that gomuti palm seed is sprouted, and the bottom of the culture dish is coated with photocatalyst, described rush
Enter the method for gomuti palm seed sprouting, in addition to：
After with sealed membrane, the culture dish is sealed, when being cultivated daily under illumination condition, the training described in ultraviolet light
Support the bottom 3min of ware, Continuous irradiation 90 days.Photocatalyst can effectively kill various bacteria under the irradiation of ultraviolet light, and can be by carefully
The toxin that bacterium or fungi discharge decomposes and harmless treatment, also can further prevent that culture dish bottom is mouldy.
Embodiment 2
The present invention provides a kind of method for promoting gomuti palm seed to sprout, and comprises the following steps：
Step 1: it is 1 by volume by gomuti palm seed and wet perlite:3 is well mixed, after sealing, the lamination at 15 DEG C
90 days, obtain the gomuti palm seed after lamination；Stratificated treatment can release seed dormancy, promote seed to sprout.
Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, the drying equipped with dry silica gel is placed in
In device, drying at room temperature 3h, dry gomuti palm seed is obtained.Before culture is sprouted, some dewater treatments, Ke Yishi are carried out to seed
When offer germination rate.
In the described method for promoting gomuti palm seed to sprout, the wet perlite by 1 parts by weight perlite and 2 parts by weight
Sterilized water mixing after be made.
In the described method for promoting gomuti palm seed to sprout, gomuti palm seed described in the drier after lamination with it is described
The volume ratio of dry silica gel is 1:100.
Described promotes in the method that gomuti palm seed is sprouted, by the gomuti palm seed and the wet pearl in the step 1
Before rock mixing, in addition to the step of sterilized to the gomuti palm seed, it is concretely comprised the following steps：
The gomuti palm seed is put into after soaking 5min in the liquor potassic permanganate that mass fraction is 0.1%, taken out, with nothing
Bacterium water rinses 3 times.The gomuti palm seed of collection is sterilized, the germ carried on seed can be reduced or eliminated.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
After being sterilized in the step 1 to the gomuti palm seed, the gomuti palm seed and the wet perlite are mixed
Before conjunction, the gas-phase silica that volume is the wet perlite volume 30% is added into the wet perlite, wherein, half
The gas-phase silica absorbs 50% carbendazol wettable powder, the 300 times of liquid for having 20 times of deadweight, and second half the gas phase is white
Carbon black absorbs 2.5% pleocidin suspension, the 1500 times of liquid for having 5 times of deadweight.Gas-phase silica can be by 50% carbendazim of absorption
1500 times of liquid of 300 times of liquid of wettable powder and 2.5% pleocidin suspension slowly release, and can prevent higher concentration
The influence that medicament is sprouted to seed, and can is sprouted for seed longer provides suitable growing environment.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 3: being sterilized to dry gomuti palm seed, it is concretely comprised the following steps：
First dry gomuti palm seed is put into the ethanol water that volume fraction is 75% and soaks 0.5min, is taken out, then
It is put into the aqueous sodium hypochlorite solution that mass fraction is 1% and soaks 5min, take out, with aseptic water washing 3 times, after is sterilized
Dry gomuti palm seed.Dry gomuti palm seed is sterilized, prevents dry gomuti palm seed from carrying more bacterium, causes to sprout
The mold of seed in incubation is sent out, reduces germination percentage.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 4: sprouting culture is carried out to the gomuti palm seed of the drying after the sterilization that is obtained in step 3, its specific steps
For：
After equably laying depth is 2cm perlite in a diameter of 13cm culture dish, added into culture dish
110mL sterilized waters, then the gomuti palm seed of the drying after 50 sterilizations is placed in culture dish, it is well mixed with perlite,
Culture dish is sealed with sealed membrane afterwards, at 30 DEG C, 12h is first cultivated under illumination condition daily, then trained under dark condition
Support 12h, continuous culture 90 days.
Described promotes in the method that gomuti palm seed is sprouted, in step 4, in addition to：
Before perlite being laid in the culture dish, the wheat for being 1cm to the bottom even laying depth of the culture dish
Meal stone ball, and 15g 50% carbendazol wettable powder, 150 times of liquid are poured into culture dish, afterwards on the medical stone ball
One layer of laying is laid on the filter paper with culture dish diameter identical filter paper, perlite；Sprout in incubation, moisture can be by
Gradually to the bottom lamination of culture dish, cause culture dish bottom easily mouldy, medical stone ball, medical stone ball energy are set in culture dish bottom
Adsorb bacterium, and 50% carbendazol wettable powder, 150 times of liquid poured into culture dish, medical stone ball can absorb moisture and
50% 150 times of carbendazol wettable powder liquid, and the medicament of absorption is slowly released, it can guarantee that the bottom of culture dish is in
Germ-free condition.
After the gomuti palm seed of drying after sterilization is placed in culture dish, before being well mixed with perlite, to pearl
In rock add 20g absorptions have deadweight 300 times of bactericide water-loss reducer, afterwards with perlite and sterilize after drying gomuti palm kind
Son is well mixed, and 10g glycerine is uniformly sprayed on the surface of most backward perlite, wherein, the bactericide is by following parts by weight
Component be made：30 parts of 0.3 part of lysozyme, 0.5 part of lywallzyme, 0.2 part of chitosan, 3 parts of pyrolkigneous liquid and water.Lysozyme can make carefully
Bacterium cell wall rupture, can also with negatively charged virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make
It is virally inactivated.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and the temperature for sprouting culture is 30 DEG C, because
And lywallzyme can be made to be in suitable hydrolysis temperature.Chitosan has bacteriostatic activity.Pyrolkigneous liquid can suppress the breeding of miscellaneous bacteria, tool
There is the effect of fungi-proofing, insect prevention, acetic acid content is higher in pyrolkigneous liquid, and solution can dissolve chitosan in acidity, and makes lysozyme and molten
Wall enzyme is in suitable sour environment, improves enzymolysis efficiency.
Described promotes in the method that gomuti palm seed is sprouted, and the bottom of the culture dish is coated with photocatalyst, described rush
Enter the method for gomuti palm seed sprouting, in addition to：
After with sealed membrane, the culture dish is sealed, when being cultivated daily under illumination condition, the training described in ultraviolet light
Support the bottom 5min of ware, Continuous irradiation 90 days.Photocatalyst can effectively kill various bacteria, and bacterium or fungi can be discharged
Toxin decomposes and harmless treatment, also can further prevent that culture dish bottom is mouldy.
Embodiment 3
The present invention provides a kind of method for promoting gomuti palm seed to sprout, and comprises the following steps：
Step 1: it is 1 by volume by gomuti palm seed and wet perlite:3 is well mixed, after sealing, the lamination at 12 DEG C
90 days, obtain the gomuti palm seed after lamination；Stratificated treatment can release seed dormancy, promote seed to sprout.
Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, the drying equipped with dry silica gel is placed in
In device, drying at room temperature 3h, dry gomuti palm seed is obtained.Before culture is sprouted, some dewater treatments, Ke Yishi are carried out to seed
When offer germination rate.
In the described method for promoting gomuti palm seed to sprout, the wet perlite by 1 parts by weight perlite and 1.7 weight
It is made after the sterilized water mixing of part.
In the described method for promoting gomuti palm seed to sprout, gomuti palm seed described in the drier after lamination with it is described
The volume ratio of dry silica gel is 1:100.
Described promotes in the method that gomuti palm seed is sprouted, by the gomuti palm seed and the wet pearl in the step 1
Before rock mixing, in addition to the step of sterilized to the gomuti palm seed, it is concretely comprised the following steps：
The gomuti palm seed is put into after soaking 5min in the liquor potassic permanganate that mass fraction is 0.1%, taken out, with nothing
Bacterium water rinses 3 times.The gomuti palm seed of collection is sterilized, the germ carried on seed can be reduced or eliminated.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
After being sterilized in the step 1 to the gomuti palm seed, the gomuti palm seed and the wet perlite are mixed
Before conjunction, the gas-phase silica that volume is the wet perlite volume 25% is added into the wet perlite, wherein, half
The gas-phase silica absorbs 50% carbendazol wettable powder, the 250 times of liquid for having 15 times of deadweight, and second half the gas phase is white
Carbon black absorbs 2.5% pleocidin suspension, the 1250 times of liquid for having 4 times of deadweight.Gas-phase silica can be by 50% carbendazim of absorption
1250 times of liquid of 250 times of liquid of wettable powder and 2.5% pleocidin suspension slowly release, and can prevent higher concentration
The influence that medicament is sprouted to seed, and can is sprouted for seed longer provides suitable growing environment.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 3: being sterilized to dry gomuti palm seed, it is concretely comprised the following steps：
First dry gomuti palm seed is put into the ethanol water that volume fraction is 75% and soaks 0.5min, is taken out, then
It is put into the aqueous sodium hypochlorite solution that mass fraction is 1% and soaks 5min, take out, with aseptic water washing 3 times, after is sterilized
Dry gomuti palm seed.Dry gomuti palm seed is sterilized, prevents dry gomuti palm seed from carrying more bacterium, causes to sprout
The mold of seed in incubation is sent out, reduces germination percentage.
Described promotes in the method that gomuti palm seed is sprouted, in addition to：
Step 4: sprouting culture is carried out to the gomuti palm seed of the drying after the sterilization that is obtained in step 3, its specific steps
For：
After equably laying depth is 1.5cm perlite in a diameter of 13cm culture dish, added into culture dish
105mL sterilized waters, then the gomuti palm seed of the drying after 50 sterilizations is placed in culture dish, it is well mixed with perlite,
Culture dish is sealed with sealed membrane afterwards, at 30 DEG C, 12h is first cultivated under illumination condition daily, then trained under dark condition
Support 12h, continuous culture 90 days.
Described promotes in the method that gomuti palm seed is sprouted, in step 4, in addition to：
It is 0.9cm's to the bottom even laying depth of the culture dish before laying perlite in the culture dish
Medical stone ball, and 12g 50% carbendazol wettable powder, 125 times of liquid are poured into culture dish, afterwards in the medical stone ball
Upper one layer of laying is laid on the filter paper with culture dish diameter identical filter paper, perlite；Sprout in incubation, moisture meeting
Gradually to the bottom lamination of culture dish, cause culture dish bottom easily mouldy, medical stone ball, medical stone ball are set in culture dish bottom
Bacterium can be adsorbed, and 50% carbendazol wettable powder, 125 times of liquid have been poured into culture dish, medical stone ball can absorb moisture and
50% 125 times of carbendazol wettable powder liquid, and the medicament of absorption is slowly released, it can guarantee that the bottom of culture dish is in
Germ-free condition.
After the gomuti palm seed of drying after sterilization is placed in culture dish, before being well mixed with perlite, to pearl
In rock add 15g absorptions have deadweight 250 times of bactericide water-loss reducer, afterwards with perlite and sterilize after drying gomuti palm kind
Son is well mixed, and 7g glycerine is uniformly sprayed on the surface of most backward perlite, wherein, the bactericide is by following parts by weight
Component is made：25 parts of 0.25 part of lysozyme, 0.4 part of lywallzyme, 0.15 part of chitosan, 2 parts of pyrolkigneous liquid and water.Lysozyme can make carefully
Bacterium cell wall rupture, can also with negatively charged virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make
It is virally inactivated.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and the temperature for sprouting culture is 30 DEG C, because
And lywallzyme can be made to be in suitable hydrolysis temperature.Chitosan has bacteriostatic activity.Pyrolkigneous liquid can suppress the breeding of miscellaneous bacteria, tool
There is the effect of fungi-proofing, insect prevention, acetic acid content is higher in pyrolkigneous liquid, and solution can dissolve chitosan in acidity, and makes lysozyme and molten
Wall enzyme is in suitable sour environment, improves enzymolysis efficiency.
Described promotes in the method that gomuti palm seed is sprouted, and the bottom of the culture dish is coated with photocatalyst, described rush
Enter the method for gomuti palm seed sprouting, in addition to：
After with sealed membrane, the culture dish is sealed, when being cultivated daily under illumination condition, the training described in ultraviolet light
Support the bottom 4min of ware, Continuous irradiation 90 days.Photocatalyst can effectively kill various bacteria, and bacterium or fungi can be discharged
Toxin decomposes and harmless treatment, also can further prevent that culture dish bottom is mouldy.
Comparative example 1
1) collect seed, sterilize：In September, 2014, the gomuti palm fruit of maturation is harvested in Medicinal Plants of Guangxi garden, seed is stripped out
Rinsed well again with the running water of flowing afterwards, interior is dried in the shade 1 day, and sieve takes 400 hard, seed of grey black, is divided into eight
Group, the liquor potassic permanganate soaking disinfection that every group of mass fraction with 300mL is 0.1% 5 minutes, is used after filtering out gomuti palm seed
Distilled water flushing 3 times, carry out first time seed disinfection.
2) the gomuti palm seed after four groups are sterilized for the first time is respectively 1 by volume with wet perlite:3 is well mixed, close
It is honored as a queen, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after four groups of laminations, four groups is not done Stratificated treatment in addition.Wherein, wet treasure
Zhu Yan is well mixed and obtained by the perlite of 1 parts by weight and the water of 1.7 parts by weight.
3) dewater treatment：The gomuti palm seed that gomuti palm seed after four groups of laminations and four groups are not done into Stratificated treatment respectively with
Dehydration 3 hours in drier are placed in after the mixing of 3000g silica gel.
4) culture is sprouted：By four groups successively gomuti palm seed after lamination and dewater treatment take out, labeled as CJ1,
CJ2、CJ3、CJ4；By four groups without Stratificated treatment, but gomuti palm seed after dewater treatment takes out, labeled as CK1, CK2,
CK3、CK4.Eight groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts are 75% respectively afterwards, then divided with quality
Number is soaked seed 5 minutes for 1% aqueous sodium hypochlorite solution, is finally rinsed 3 times with sterile distilled water, carries out second of seed disinfection.
Every group of gomuti palm seed after second is sterilized uniformly is put in glass culture dish (pre- in a diameter of 13cm glass culture dish
Perlite is first paved with, is handled 20 minutes by 120 DEG C of high-temperature sterilizations), culture 90 in illumination box is placed in after sealed membrane sealing
My god.Illumination box daily 8 is set：00 to 20:00 carries out illumination, and intensity of illumination is 1000~3000lx, cultivation temperature 30
℃；Remaining time is dark, and cultivation temperature is 30 DEG C.
Every group is accounted for the prominent gomuti palm seed grain number to break in the seed coat of radicle be designated as seed for planting experimentally the percentage of seed number after 90 days
Germination rate, the seed germination rate of CJ1, CJ2, CJ3, CJ4 through Stratificated treatment are respectively 68%, 78%, 85%, 73%, average
Germination rate is up to 76%；And the seed germination rate difference 2%, 6%, 0%, 2% of CK1, CK2, CK3, CK4 without Stratificated treatment,
Average germination rate is up to 2.5%.Illustrate to make the germination rate of gomuti palm seed improve 30.4 times by Stratificated treatment.
Comparative example 2
1) collect seed, sterilize：In September, 2014, the gomuti palm fruit of maturation is harvested in Medicinal Plants of Guangxi garden, seed is stripped out
Rinsed well again with the running water of flowing afterwards, interior is dried in the shade 1 day, and sieve takes 400 hard, seed of grey black, is divided into eight
Group, the liquor potassic permanganate soaking disinfection that every group of mass fraction with 300mL is 0.1% 5 minutes, is used after filtering out gomuti palm seed
Distilled water flushing 3 times.
2) the gomuti palm seed after eight groups are sterilized for the first time is respectively 1 by volume with wet perlite:3 is well mixed, close
It is honored as a queen, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after eight groups of laminations.Wherein, wet perlite by 1 parts by weight perlite
It is well mixed and obtains with the water of 1.7 parts by weight.
3) dewater treatment：It is placed in drier and takes off after gomuti palm seed after eight groups of laminations is mixed with 3000g silica gel respectively
Water 3 hours.
4) culture is sprouted：By four groups successively gomuti palm seed after lamination and dewater treatment take out, labeled as CA1,
CA2、CA3、CA4；By other four groups successively gomuti palm seed after lamination and dewater treatment take out, labeled as CB1, CB2,
CB3、CB4.Eight groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts are 75% respectively afterwards, then divided with quality
Number is soaked seed 5 minutes for 1% aqueous sodium hypochlorite solution, is finally rinsed 3 times with sterile distilled water.Every group after second is sterilized
Gomuti palm seed is uniformly put in glass culture dish (is paved with perlite, by 120 in advance in a diameter of 13cm glass culture dish
DEG C high-temperature sterilization is handled 20 minutes), culture 90 days in illumination box are placed in after sealed membrane sealing.CA1, CA2, CA3, CA4 group
Illumination box daily 8 is set：00 to 20:00 carries out illumination, and intensity of illumination is 1000~3000lx, and cultivation temperature is 30 DEG C；
Remaining time is dark, and cultivation temperature is 30 DEG C.CB1, CB2, CB3, CB4 group set illumination box daily 8：00 to 20:00
Illumination is carried out, intensity of illumination is 1000~3000lx, and cultivation temperature is 30 DEG C；Remaining time is dark, and cultivation temperature is 20 DEG C.
Every group is accounted for the prominent gomuti palm seed grain number to break in the seed coat of radicle be designated as seed for planting experimentally the percentage of seed number after 90 days
Germination rate, the seed germination rate of CA1, CA2, CA3, CA4 group is respectively 83%, 66%, 77%, 63%, and average germination rate reaches
72.25%；And the seed germination rate difference 8%, 0%, 3%, 1% of CB1, CB2, CB3, CB4 group, average germination rate is up to 3%.Say
Cultivation temperature maintains 30 DEG C always in bright sprouting incubation can make the germination rate of gomuti palm seed improve 24 times.
Comparative example 3
1) collect seed, sterilize：In September, 2014, the gomuti palm fruit of maturation is harvested in Medicinal Plants of Guangxi garden, seed is stripped out
Rinsed well again with the running water of flowing afterwards, interior is dried in the shade 1 day, and sieve takes 400 hard, seed of grey black, is divided into eight
Group, wherein four groups of mass fractions with 300mL are 0.1% liquor potassic permanganate soaking disinfection 5 minutes, filter out gomuti palm seed
Distilled water flushing is used afterwards 3 times.
2) the gomuti palm seed after four groups are sterilized for the first time is respectively 1 by volume with wet perlite:3 is well mixed, close
It is honored as a queen, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after four groups of laminations.Wherein, wet perlite by 1 parts by weight perlite
It is well mixed and obtains with the water of 1.7 parts by weight.
3) dewater treatment：It is placed in drier and takes off after gomuti palm seed after four groups of laminations is mixed with 3000g silica gel respectively
Water 3 hours.
4) culture is sprouted：By four groups successively gomuti palm seed after lamination and dewater treatment take out, labeled as CM1,
CM2, CM3, CM4, this four groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts are 75% respectively afterwards, then used
The aqueous sodium hypochlorite solution that mass fraction is 1% is soaked seed 5 minutes, is finally rinsed 3 times with sterile distilled water.After sterilizing for second
Every group of gomuti palm seed uniformly put in glass culture dish (be paved with perlite in advance in a diameter of 13cm glass culture dish,
Handled 20 minutes by 120 DEG C of high-temperature sterilizations), culture 90 days in illumination box are placed in after sealed membrane sealing.Other four groups of marks
CN1, CN2, CN3, CN4 are designated as, it is without any processing to end to beginning, every group of gomuti palm seed is uniformly put in glass culture dish
(being paved with perlite in advance in a diameter of 13cm glass culture dish, handle 20 minutes by 120 DEG C of high-temperature sterilizations), sealed membrane envelope
Culture 90 days in illumination box are placed in after mouthful.Often setting illumination box daily 8 in eight groups：00 to 20:00 carries out illumination,
Intensity of illumination is 1000~3000lx, and cultivation temperature is 30 DEG C；Remaining time is dark, and cultivation temperature is 30 DEG C.
Every group is accounted for the prominent gomuti palm seed grain number to break in the seed coat of radicle be designated as seed for planting experimentally the percentage of seed number after 90 days
Germination rate, the seed germination rate of CM1, CM2, CM3, CM4 group through Stratificated treatment is respectively 56%, 66%, 58%, 76%, is put down
Equal germination rate is up to 64%；And CN1, CN2, CN3, CN4 group without any processing seed germination rate difference 2%, 6%, 0%,
2%, average germination rate is up to 2.5%.Illustrate to make gomuti palm kind after first time sterilizes, crosses Stratificated treatment, second of sterilamp processing
The germination rate of son improves 25.6 times.
Comparative example 4
Four groups of gomuti sugarpalm fruit seeds of method culture as shown in embodiment 2, labeled as CP1, CP2, CP3, CP4, its seed is sprouted
Hair rate is respectively 92%, 95%, 90%, 97%, and average germination rate is up to 93.5%；CJ1, CJ2, CJ3, CJ4's in comparative example 1
Seed germination rate is respectively 68%, 78%, 85%, 73%, and average germination rate illustrates that the present invention can further improve kind up to 76%
The germination rate of son.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (1)
- A kind of 1. method for promoting gomuti palm seed to sprout, it is characterised in that comprise the following steps：Step 1: gomuti palm seed is put into the liquor potassic permanganate that mass fraction is 0.1% after immersion 5min, take out, with nothing Bacterium water rinses 3 times；Wet perlite is made after the perlite of 1 parts by weight and the sterilized water of 2 parts by weight are mixed, then to wet pearl The gas-phase silica that volume is wet perlite volume 30% is added in rock, wherein, the gas-phase silica of half, which absorbs, to be had certainly 20 times 50% carbendazol wettable powder, 300 times of liquid are weighed, second half the gas-phase silica, which absorbs, 5 times of deadweight 2.5% 1500 times of pleocidin suspension liquid, afterwards by gomuti palm seed with being mixed with the wet perlite of gas-phase silica by volume Than for 1:3 is well mixed, after sealing, lamination 90 days at 15 DEG C, obtains the gomuti palm seed after lamination；Step 2: after the gomuti palm seed after the lamination is fitted into kraft paper bag, it is placed in the drier equipped with dry silica gel, The volume ratio of gomuti palm seed and dry silica gel in the drier after lamination is 1:100,3h is then dried at room temperature for, is obtained Dry gomuti palm seed；Step 3: being sterilized to dry gomuti palm seed, it is concretely comprised the following steps：Dry gomuti palm seed is put into after soaking 0.5min in the ethanol water that volume fraction is 75%, takes out, place into Mass fraction is to soak 5min in 1% aqueous sodium hypochlorite solution, is taken out, with aseptic water washing 3 times, the gomuti palm after being sterilized Seed；Step 4: carrying out sprouting culture to the gomuti palm seed after being sterilized in step 3, it is concretely comprised the following steps：It is 1cm's in the bottom even laying depth of culture dish after the bottom coating photocatalyst of a diameter of 13cm culture dish Medical stone ball, and 15g 50% carbendazol wettable powder, 150 times of liquid are poured into culture dish, laid afterwards on medical stone ball One layer with culture dish diameter identical filter paper, and on filter paper equably laying depth be 2cm perlite after, into culture dish 110mL sterilized waters are added, then the gomuti palm seed after being sterilized in 50 step 3 and 20g absorptions there are into 300 times of bactericide of deadweight Water-loss reducer is placed in culture dish, is well mixed with perlite, then uniformly sprays 10g glycerine to the surface of perlite, then with envelope Membrana oralis seals culture dish, and at 30 DEG C, 12h is first cultivated under illumination condition daily, then cultivates 12h under dark condition, even When continuing culture 90 days, and being cultivated daily under illumination condition, the bottom 5min of culture dish, Continuous irradiation described in ultraviolet light 90 days；Wherein, the bactericide is made up of the component of following parts by weight：0.3 part of lysozyme, 0.5 part of lywallzyme, chitosan 30 parts of 0.2 part, 3 parts of pyrolkigneous liquid and water.
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| CN107278553B (en) * | 2017-05-26 | 2020-08-25 | 陈志峰 | High-yield planting method for hot peppers |
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| CN103891451A (en) * | 2012-12-26 | 2014-07-02 | 海口同方阳光科技有限公司 | Germination accelerating method for Hainan rattan seeds |
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| CN103891451A (en) * | 2012-12-26 | 2014-07-02 | 海口同方阳光科技有限公司 | Germination accelerating method for Hainan rattan seeds |
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