CN1568653A - Factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof - Google Patents
Factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof Download PDFInfo
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- CN1568653A CN1568653A CN 200410027237 CN200410027237A CN1568653A CN 1568653 A CN1568653 A CN 1568653A CN 200410027237 CN200410027237 CN 200410027237 CN 200410027237 A CN200410027237 A CN 200410027237A CN 1568653 A CN1568653 A CN 1568653A
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Abstract
The invention provides a factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof, wherein the production method mainly comprises the steps of bacterial production and sclerotium cultivation administration, the invention is characterized in that, culturing the fungus core tissue on shaking table in the liquid medium, thus greatly shortening the bacterial culture time. The liquid medium ingredients are also disclosed in the invention.
Description
Technical field:
The present invention relates to the industrial production method of Pleurotus tuber-regium sclerotium and relevant medium mixture.
Background technology:
The Pleurotus tuber-regium sclerotium is tropical edible mushroom, and main product is in Africa, in the torrid areas, Asia and Australia distribution is also arranged.In some countries and regions edible tradition is arranged, also can be used as medicine among the people: be used for the treatment of stomachache, constipation, fever, flu, oedema, pectoralgia, nervous system disease, smallpox, asthma, reach diseases such as hypertension, diabetes, and can promote development of fetus, improve premature's survival rate.Along with improving day by day and pharmaceutically extensive use of people's living standard, with increasing, only depend on wild Pleurotus tuber-regium sclerotium not satisfy people's demand far away to the demand of Pleurotus tuber-regium sclerotium.In recent years, the report of existing artificial cultivation Pleurotus tuber-regium sclerotium.
The production method of the Pleurotus tuber-regium sclerotium of prior art is the separate tissue acquisition bacterial classification by sclerotium or fruit body, cultivate with film solid media, problems such as the ubiquity spawn culture time is long, cultivating bacterial spawn harvest rate is low, vegetative period is long, sclerote is little, yield poorly, and because used organizer's difference, easily cause growth scale, the performance of sclerotium different, long-term use will cause the degeneration of the production traits.Therefore, prior art is difficult to form the large-scale production ability.
Summary of the invention:
The objective of the invention is to overcome the weak point that prior art is produced the Pleurotus tuber-regium sclerotium, a kind of method of energy large-scale production Pleurotus tuber-regium sclerotium and relevant medium mixture are provided.
The industrial production method of Pleurotus tuber-regium sclerotium mainly comprises: the Pleurotus tuber-regium bacterial classification is made and two stages of Pleurotus tuber-regium sclerotium cultivation management.
Pleurotus tuber-regium bacterial classification preparation method of the present invention, key step comprises:
A, obtaining liq medium: its formulation weight than component is: analysis for soybean powder 3-8%; Ginseng pulverate 0.1-2%; Glucose 3-8%; Cellulose 5-10%; VB
10.1-2%; Calcium chloride 1-3%; Magnesium sulfate 1-3%; Potassium dihydrogen phosphate 1-3%; Peptone 20-30%; Water surplus.
B, liquid nutrient medium sterilization: the liquid nutritional base with steps A obtains, in the triangular flask of packing into, seal with the cotton pad of gauze, wrap up brown paper, retort sterilization is 1 hour in the sterilization pressure cooker, and the cooling back moves into the interior ultraviolet lamp irradiation of superclean bench 30-40 minute;
C, choose female sclerotium of planting: getting mellow and full, solid, the fresh and alive Pleurotus tuber-regium sclerotium water of entity, to rinse the back well stand-by;
D, female sclerotium of planting are sterilized: the mother that will clean plants sclerotium on superclean bench, placed 75% alcohol-pickled 8-12 minute with conventional sterile working method, soaked 10-15 minute with 0.1% mercuric chloride solution again, use aseptic water washing 4-5 time, and blot its surface moisture with filter paper;
E, making sclerotium tissue: the Pleurotus tuber-regium sclerotium after will sterilizing is removed crust with pocket knife, is cut into the square shape sclerotium tissue of about 1cm then, generally gets the 20-40 piece;
F, tissue culture: will send in the triangular flask with the tweezers dress at the sclerotium tissue that superclean bench is made, the cotton pad of gauze seals, wrapping brown paper, place on the rotary shaker, rotating speed is 1000 rev/mins, 28 ± 2 ℃ indoor cultivation 4-6 days, when liquid nutrient medium in the triangular flask is covered with white bacterium ball or mycelium liquid and becomes thick, promptly obtain the Pleurotus tuber-regium liquid spawn.
Pleurotus tuber-regium sclerotium cultivation method of the present invention, key step comprises;
A, preparation solid culture medium, its formulation weight than component is: wood chip 30-50%; Wheat bran 40-60%; Ginseng leaf powder 1-2%; Sucrose 1-3%; Calcium carbonate 1-2%.
The making of B, cultivation bag, sterilization and inoculation: with the plastic sack of about 17 * 33cm, the steps A of packing into solid culture medium after the routine sterilization, cools off standby; In sterile board, each cultivation bag is connected the Pleurotus tuber-regium bacterial classification then, move on to then in 20-25 ℃ the greenhouse and cultivate.
C, cultivation management: after inoculation survives, cultivation bag is emitted on the bedstead of cultivating chamber, temperature is controlled between 25-35 ℃, humidity 75%-95% cultivated after 30-40 days, and pure white mycelia sends out purseful, subsequently above composts or fertilisers of cultivating or middle mycelia begin to assemble, form the Pleurotus tuber-regium sclerotium,, can slough the plastics collar when sclerotium soon during the bursting plastic sack, take out tampon, unclamp sack, prevent that plastic sack from breaking, subsequently, its humidity can improve relatively, acutely contraction of composts or fertilisers of cultivating, water outlet, deliquescing after about 4 months, sclerotium just can not gathered after can not continuing to grow up successively.
The present invention, its good effect is:
1, directly places liquid nutrient medium on shaking table, to cultivate in the sclerotium tissue, both obtained batch production and produced required a large amount of bacterial classifications, shortened the culture of strains time greatly again.The present invention organizes to obtaining bacterial classification from sclerotium and only needs about 5 days time, is 23-25 days and adopt conventional method such as solid culture medium to obtain bacterial classification.
2, femalely plant the sclerotium sterilization and adopt " ethanol+mercuric chloride+aseptic water washing " method, its culture success ratio is more than 95%, and adopts " ethanol+liquor natrii hypochloritis+aseptic water washing " method, and its culture success ratio is generally below 70%.
3, because solid nutrition basigamy material is reasonable, Pleurotus tuber-regium bacteria growing reproduction speed is accelerated, can gather in the crops sclerotium in 4 months, not only shorten growth periodicity, and improved single sclerotium output more than 30%.
4, medium of the present invention is raw materials used cheap, be available anywhere, and cultivation method is simple, and less investment, instant effect is adapted at the mountain area and applies, large-scale industrialized cultivation and production.
Embodiment:
Embodiment 1, be used to produce the liquid nutrient medium of Pleurotus tuber-regium bacterial classification.
The formulation weight of liquid nutrient medium than component is: analysis for soybean powder 4%; The ginseng pulverate 1%; Glucose 5%; Cellulose 8%; VB
10.5%; Calcium chloride 2%; Magnesium sulfate 2%; Potassium dihydrogen phosphate 2%; Peptone 22%; Water surplus.
Embodiment 2, Pleurotus tuber-regium bacterial classification preparation method.
Key step comprises:
A, obtaining liq medium: with embodiment 1 gained liquid nutrient medium as Pleurotus tuber-regium culture of strains base.
B, liquid nutrient medium sterilization: the liquid nutritional base of embodiment 1 gained is packed in the 5000ml triangular flask, seal with the cotton pad of gauze, wrapping brown paper, (121 ℃) retort sterilization is 1 hour in the sterilization pressure cooker, and the ultraviolet lamp irradiation is 35 minutes in the cooling back immigration superclean bench.
C, choose female sclerotium of planting: getting mellow and full, solid, the fresh and alive Pleurotus tuber-regium sclerotium water of entity, to rinse the back well stand-by.
D, female plant the sclerotium sterilization: the mother that will clean plants sclerotium on superclean bench, places 75% alcohol-pickled 10 minutes with conventional sterile working method, with 0.1% mercuric chloride solution immersion 15 minutes, uses aseptic water washing 5 times again, and blots its surface moisture with filter paper.
E, making sclerotium tissue: the Pleurotus tuber-regium sclerotium after will sterilizing is removed crust with pocket knife, is cut into the square shape sclerotium tissue of about 1cm then, chooses 30.
F, tissue culture: will send in the triangular flask of packing into tweezers at the sclerotium tissue that superclean bench is made, the cotton pad of gauze seals, wrapping brown paper, place on the rotary shaker, rotating speed is 1000 rev/mins, 28 ± 2 ℃ of indoor cultivation 5 days when liquid nutrient medium in the triangular flask is covered with white bacterium ball or mycelium liquid and becomes thick, promptly obtain the Pleurotus tuber-regium liquid spawn.
Embodiment 3, be used to cultivate the solid culture medium of Pleurotus tuber-regium sclerotium.
Its formulation weight than component is: wood chip 42%; Wheat bran 50%; Ginseng leaf powder 2%; Sucrose 3%; Calcium carbonate 3%.With the formula ratio component fully mixed after, add the water allotment and make it moistening, the amount that generally adds water is not dripped and is advisable to hold medium.
Embodiment 4, Pleurotus tuber-regium sclerotium cultivation method.
Key step comprises:
A, preparation medium: with embodiment 3 gained solid culture mediums, as the solid culture medium of cultivation Pleurotus tuber-regium sclerotium.
The making of B, cultivation bag, sterilization and inoculation: with the polypropylene plastics pocket of about 17 * 33cm, the embodiment 3 gained solid culture mediums of packing into, after the routine sterilization, cool off standby, in sterile board, each cultivation bag is connected embodiment 2 gained Pleurotus tuber-regium bacterial classifications then, move on to again in 20-25 ℃ the greenhouse and cultivate.
C, cultivation management: after inoculation survives, check that at first bacterial classification survives situation, if bacterial classification turns white, the bacterium group is recovered growth, do not infect other assorted bacterium again, just cultivation bag can be emitted on the bedstead of cultivating chamber, indoor temperature is controlled between 25-35 ℃, and humidity is 75%-95%, cultivate after 30-40 days, pure white mycelia sends out purseful, subsequently above composts or fertilisers of cultivating or middle mycelia begin to assemble, form the Pleurotus tuber-regium sclerotium.When sclerotium soon during the bursting plastic sack, can slough the plastics collar, take out tampon, unclamp sack, prevent that plastic sack from breaking, subsequently, its humidity can improve relatively, acutely contraction of composts or fertilisers of cultivating, water outlet, deliquescing after about 4 months, sclerotium just can not gathered after can not continuing to grow up successively.
In this example, embodiment 2 gained bacterial classifications directly can be seeded on the embodiment 3 gained medium, inoculate cultivation after also can earlier liquid spawn being generated the original seed bacterial classification: by embodiment 3 gained medium, the seed bottle of packing into or plastic sack put plastic hoop, tampon beyond the Great Wall, after routine sterilization, cool off standby, with conventional disinfecting action method, access is treated promptly to obtain the original seed bacterial classification after full bottle of mycelial growth or the purseful by embodiment 2 gained liquid spawns.
Claims (4)
1, a kind of liquid nutrient medium that is used to produce the Pleurotus tuber-regium bacterial classification, its formulation weight than component is: analysis for soybean powder 3-8%; Ginseng pulverate 0.1-2%; Glucose 3-8%; Cellulose 5-10%; VB1 0.1-2%; Calcium chloride 1-3%; Magnesium sulfate 1-3%; Potassium dihydrogen phosphate 1-3%; Peptone 20-30%; Water surplus.
2, a kind of Pleurotus tuber-regium bacterial classification preparation method, key step comprises:
A, obtaining liq medium, its formulation weight than component is: analysis for soybean powder 3-8%; Ginseng pulverate 0.1-2%; Glucose 3-8%; Cellulose 5-10%; VB1 0.1-2%; Calcium chloride 1-3%; Magnesium sulfate 1-3%; Potassium dihydrogen phosphate 1-3%; Peptone 20-30%; Water surplus;
B, liquid nutrient medium sterilization: the liquid nutritional base with steps A obtains, in the triangular flask of packing into, seal with the cotton pad of gauze, wrap up brown paper, retort sterilization is 1 hour in the sterilization pressure cooker, and the cooling back moves into the interior ultraviolet lamp irradiation of superclean bench 30-40 minute;
C, choose female sclerotium of planting: getting mellow and full, solid, the fresh and alive Pleurotus tuber-regium sclerotium water of entity, to rinse the back well stand-by;
D, female sclerotium of planting are sterilized: the mother that will clean plants sclerotium on superclean bench, placed 75% alcohol-pickled 8-12 minute with conventional sterile working method, soaked 10-15 minute with 0.1% mercuric chloride solution again, use aseptic water washing 4-5 time, and blot its surface moisture with filter paper;
E, making sclerotium tissue: the Pleurotus tuber-regium sclerotium after will sterilizing is removed crust with pocket knife, is cut into the square shape sclerotium tissue of about 1cm then, the 20-30 piece;
F, tissue culture: will send in the triangular flask with the tweezers dress at the sclerotium tissue that superclean bench is made, the cotton pad of gauze seals, wrapping brown paper, place on the rotary shaker, rotating speed is 1000 rev/mins, 28 ± 2 ℃ indoor cultivation 4-6 days, when liquid nutrient medium in the triangular flask is covered with white bacterium ball or mycelium liquid and becomes thick, promptly obtain the Pleurotus tuber-regium liquid spawn.
3, a kind of solid culture medium that is used to cultivate the Pleurotus tuber-regium sclerotium, its formulation weight than component is: wood chip 30-50%; Wheat bran 40-60%; Ginseng leaf powder 1-2%; Sucrose 1-3%; Calcium carbonate 1-2%.
4, a kind of Pleurotus tuber-regium sclerotium cultivation method, key step comprises:
A, preparation solid culture medium, its formulation weight than component is: wood chip 30-50%; Wheat bran 40-60%; Ginseng leaf powder 1-2%; Sucrose 1-3%; Calcium carbonate 1-2%;
The making of B, cultivation bag, sterilization and inoculation: with the plastic sack of about 17 * 33cm, the steps A of packing into solid culture medium after the routine sterilization, cools off standby, in sterile board, each cultivation bag is connected the Pleurotus tuber-regium bacterial classification then, move on to then in 20-25 ℃ the greenhouse and cultivate;
C, cultivation management: after inoculation survives, cultivation bag is emitted on the bedstead of cultivating chamber, temperature is controlled between 25-35 ℃, humidity 75%-95% cultivated after 30-40 days, and pure white mycelia sends out purseful, subsequently above composts or fertilisers of cultivating or middle mycelia begin to assemble, form the Pleurotus tuber-regium sclerotium,, can slough the plastics collar when sclerotium soon during the bursting plastic sack, take out tampon, unclamp sack, prevent that plastic sack from breaking, subsequently, its humidity can improve relatively, acutely contraction of composts or fertilisers of cultivating, water outlet, deliquescing after about 4 months, sclerotium just can not gathered after can not continuing to grow up successively.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920065A (en) * | 2015-05-25 | 2015-09-23 | 铜仁学院 | Preparation method for mother strain of wild dictyophora rubrovolvatain of Mount Fanjing |
| CN105165409A (en) * | 2015-10-20 | 2015-12-23 | 温炫翀 | Method for indoor industrialized production of pleurotus tuber-regium |
| CN105684734A (en) * | 2016-02-03 | 2016-06-22 | 抚州市临川金山生物科技有限公司 | Field planting method for pleurotus tuber-regium |
| CN106756320A (en) * | 2016-12-15 | 2017-05-31 | 苏州科胜仓储物流设备有限公司 | A kind of fluent formula shelf of aluminium alloy |
| CN109220545A (en) * | 2018-09-30 | 2019-01-18 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Using pine cedar sawdust as the Sparassis crispa cultivation matrix of raw material and its Sparassis crispa cultural method |
| CN109771452A (en) * | 2019-03-11 | 2019-05-21 | 河北医科大学第四医院 | Application of the Pleurotus tuber-regium extract in preparation tumor |
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2004
- 2004-05-13 CN CN 200410027237 patent/CN1253072C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920065A (en) * | 2015-05-25 | 2015-09-23 | 铜仁学院 | Preparation method for mother strain of wild dictyophora rubrovolvatain of Mount Fanjing |
| CN104920065B (en) * | 2015-05-25 | 2017-03-08 | 铜仁学院 | A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing |
| CN105165409A (en) * | 2015-10-20 | 2015-12-23 | 温炫翀 | Method for indoor industrialized production of pleurotus tuber-regium |
| CN105165409B (en) * | 2015-10-20 | 2017-10-24 | 揭阳市国康科技实业有限公司 | The method that indoor industrially produces polyporus rhinoceros cooke mushroom |
| CN105684734A (en) * | 2016-02-03 | 2016-06-22 | 抚州市临川金山生物科技有限公司 | Field planting method for pleurotus tuber-regium |
| CN106756320A (en) * | 2016-12-15 | 2017-05-31 | 苏州科胜仓储物流设备有限公司 | A kind of fluent formula shelf of aluminium alloy |
| CN109220545A (en) * | 2018-09-30 | 2019-01-18 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Using pine cedar sawdust as the Sparassis crispa cultivation matrix of raw material and its Sparassis crispa cultural method |
| CN109771452A (en) * | 2019-03-11 | 2019-05-21 | 河北医科大学第四医院 | Application of the Pleurotus tuber-regium extract in preparation tumor |
| CN109771452B (en) * | 2019-03-11 | 2021-09-21 | 河北医科大学第四医院 | Application of Pleurotus tuber-regium extract in preparing medicine for treating tumor |
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