CN105165409B - The method that indoor industrially produces polyporus rhinoceros cooke mushroom - Google Patents

The method that indoor industrially produces polyporus rhinoceros cooke mushroom Download PDF

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CN105165409B
CN105165409B CN201510678695.3A CN201510678695A CN105165409B CN 105165409 B CN105165409 B CN 105165409B CN 201510678695 A CN201510678695 A CN 201510678695A CN 105165409 B CN105165409 B CN 105165409B
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mushroom
sclerotium
pleurotus tuber
regium
polyporus rhinoceros
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CN105165409A (en
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温炫翀
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Jieyang Guokang Technology Industrial Co. Ltd.
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Mushroom Cultivation (AREA)
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Abstract

The present invention relates to a kind of method of batch production indoor production polyporus rhinoceros cooke mushroom.Mainly include:Prepare solid medium;Making, sterilizing and the inoculation of cultivating bag;The techniques such as cultivation management.Wherein, cultivation management is in two stages, first stage cultivates output Sclerotium of Pleurotus tuber regium, second stage is when Sclerotium of Pleurotus tuber regium is mature on the whole, by improving temperature and humidity, the long mushroom bud of Sclerotium of Pleurotus tuber regium is triggered, when bud ratio >=70%, temperature and humidity is reduced again to control the speed of growth of polyporus rhinoceros cooke mushroom, to ensure the quality of polyporus rhinoceros cooke mushroom.The present invention, realizes polyporus rhinoceros cooke mushroom indoor industrially, large-scale production, successfully break through and solve conventional art cultivation the polyporus rhinoceros cooke mushroom production cycle length, yield poorly, the problem that quality is unstable.

Description

The method that indoor industrially produces polyporus rhinoceros cooke mushroom
Technical field
The present invention relates to a kind of method of batch production indoor production polyporus rhinoceros cooke mushroom.
Background technology:
Sclerotium of Pleurotus tuber regium is potato wedge shape(As shown in Figure 1)Tropical edible and medical fungi, main product in Africa, Tropical Asian area with And Australia is also distributed.There is edible tradition in some countries and regions, can also be used as medicine among the people:Had a stomachache for treatment, The disease such as constipation, fever, flu, oedema, pectoralgia, the nervous system disease, smallpox, asthma and hypertension, diabetes, and can promote Enter development of fetus, improve premature's survival rate.
Brave milk ganoderma lucidum mushroom is horn-like(As shown in Figure 2)Edible and medical fungi(Mushroom), it is long on potato wedge shape Sclerotium of Pleurotus tuber regium Out, with higher medical value, its dry product is a kind of rare traditional Chinese medicine, and brave milk ganoderma lucidum mushroom composition contains amino acid, albumen Matter, sterol, alkaloid, polysaccharide etc., pharmacological action be strengthening by means of tonics, removing toxic substances convergence, change product, reducing blood lipid, Chinese medicine clinical practice in: 1. treat asthma, pulmonary emphysema, the cough of sympotoms caused by cold factors one hundred days, respiratory tract system disease etc.;2. artery sclerosis, hypertension, cerebrovas-cularaccident, The treatment of coronary heart diseases and angina pectoris etc.;3. treat a variety of cancerous swellings such as lung cancer;4. try out in myasthenia gravis;5. treat neurasthenia Show as dizzy, insomniac;6. treat gastric ulcer, indigestion chronic diseases;7. the various mushroom poisonings of solution.Brave milk ganoderma lucidum mushroom is rich Containing polysaccharide, amino acid, protein etc. and the multi mineral prime element effective to human body, can as human immunity conditioning agent, exempt from Epidemic disease initiator etc., by adjusting immune system, excites immunocyte to realize the killing to tumour and cancer cell, while will not injure Some tumour cells are had very strong inhibitory action by normal cell.
One of the present inventor once obtained a Chinese invention patent --- the cultural method of Sclerotium of Pleurotus tuber regium and its culture Base, by the preparation of culture medium, the making of cultivating bag, sterilizing, inoculation and cultivation management, obtains Sclerotium of Pleurotus tuber regium.
Chinese patent discloses a kind of segment wood cultivated method of polyporus rhinoceros cooke, and this method is included from the culture of indoor strain to room Outer plantation management, what is finally obtained is also polyporus rhinoceros cooke sclerotium --- potato bulk product, actually with above-mentioned Sclerotium of Pleurotus tuber regium For identical product, because the approach of acquisition is different, the place of production is different, and call is also just different.
Raising with living standards of the people and the pay attention to day by day to health care of body, add China's increasingly weighing to traditional Chinese medicine Depending on and progressively popularization and application pharmaceutically, in the market to Sclerotium of Pleurotus tuber regium and brave milk ganoderma lucidum mushroom the need for it is increasing, rely on Wild and artificial wood section cultivation much can not meet people's needs.Sclerotium of Pleurotus tuber regium is 21st century with brave milk ganoderma lucidum mushroom One of important nutrient source of human health existence, has important nutrition and health care function, is the higher valuable medicinal of added value, city Field has a extensive future.
From Sclerotium of Pleurotus tuber regium to horn-like brave milk ganoderma lucidum mushroom, it is mainly derived from wild harvesting, and its wild rate of output is very Low, artificial cultivation yet there are no standardization scale report.
The content of the invention:
The task of the present invention is《The cultural method and its culture medium of Sclerotium of Pleurotus tuber regium》On the basis of patent, by changing Enter culture medium and a kind of method of batch production indoor production polyporus rhinoceros cooke mushroom is provided.
The present invention, key step includes:
A, solid medium is prepared, its formulation weight is than component:Bagasse 10-15%;Wood chip 35-45%;Wheat bran 20-30%; Cotton seed hulls 20-25%;Sucrose 1-3%;Calcium carbonate 1-2%;
B, the making of cultivating bag, sterilizing and inoculation:Using polybag, load step A solid mediums, through conventional sterilant Afterwards, cooling is standby, each cultivating bag then is connected into Pleurotus tuber-regium strain in sterile board, in the greenhouse for then moving on to 18-25 DEG C Culture;
C, cultivation management I:After inoculation is survived, cultivating bag is emitted on the bedstead of cultivating chamber, temperature control is at 20-35 DEG C Between, humidity 75-80%, after culture 30-40 days, pure white mycelia sends out purseful, then above compost or middle mycelia starts to collect Knot, forms Sclerotium of Pleurotus tuber regium, when sclerotium soon bursting polybag, can slough Plastibell, takes out tampon, unclamps sack, Prevent polybag from rupturing, after 4 months, a Sclerotium of Pleurotus tuber regium being mature on the whole is can obtain in each cultivating bag;
D, cultivation management II:Temperature is brought up to 36-37 DEG C, humidity brings up to 90-92%, after 1-2 days, Sclerotium of Pleurotus tuber regium Surface grows the mushroom bud of white, when bud ratio >=90%, then temperature readjustment is arrived into 28-32 DEG C, and humidity readjustment arrives 75-85%, then By the culture of 10-15 days, the polyporus rhinoceros cooke mushroom grown can just harvest, and it is finished product to dry after doing.
The present invention, realizes polyporus rhinoceros cooke mushroom indoor industrially, large-scale production, has unified production standard, can give birth on a large scale Production, can both produce brave newborn mushroom sclerotium, polyporus rhinoceros cooke mushroom can be further produced again, successfully break through and solve conventional art cultivation tiger Newborn mushroom sclerotium and polyporus rhinoceros cooke mushroom production cycle length, yield poorly, the problem that quality is unstable, particularly solve traditional production During inevitably by corresponding soil the problem of containing heavy metal and pesticide remnant.And the finished product of this technology production exists Whole production process of cultivating is completed in factory building, and production process whole process obtains effective monitoring, and greatly avoiding may be contaminated Link, product is had a very big controllability, the quality and safety of product are effectively guaranteed.Realize indoor factory's life Production, is the breakthrough to artificial wood section cultivation's polyporus rhinoceros cooke sclerotium technology, meets environmental protection requirement, social benefit is notable.
Brief description of the drawings
Fig. 1 is the photo for the Sclerotium of Pleurotus tuber regium plucked in production process of the present invention;Fig. 2 is the brave milk spirit obtained by the present invention The photo of sesame mushroom.
Embodiment
A kind of method that embodiment 1, indoor industrially produce Sclerotium of Pleurotus tuber regium, key step includes:
A, solid medium is prepared, its formulation weight is than component:Bagasse 15%;Wood chip 38%;Wheat bran 21%;Cotton seed hulls 23%;Sucrose 1.5%;Calcium carbonate 1.5%;After formula ratio component is fully mixed, the allotment that adds water moistens it, the amount typically added water Do not dripped with to hold culture medium and be advisable;
B, the making of cultivating bag, sterilizing and inoculation:Using 17 × 33cm polypropylene plastics pocket, load the training of step A solids Base is supported, after conventional sterilant, cooling is standby, and each cultivating bag then is connected into Pleurotus tuber-regium strain in sterile board, then moved on to Cultivated in 20-25 DEG C of greenhouse;
C, cultivation management:After inoculation is survived, first check for strain and survive situation, if strain turns white, bacterium group restoration ecosystem, again Do not infect other miscellaneous bacterias, it is possible to which cultivating bag is emitted on the bedstead of cultivating chamber, indoor temperature control 25-35 DEG C it Between, humidity 75-80%, after culture 30-40 days, pure white mycelia sends out purseful, then above compost or middle mycelia starts to collect Knot, forms Sclerotium of Pleurotus tuber regium, when sclerotium soon bursting polybag, can slough Plastibell, takes out tampon, unclamps sack, Prevent polybag from rupturing, after 4 months, the Sclerotium of Pleurotus tuber regium in cultivating bag is mature on the whole, it is possible to harvest, and obtains Pleurotus tuber-regium bacterium Core, as shown in Figure 1.
The judgement that Sclerotium of Pleurotus tuber regium is mature on the whole:When the compost contraction in cultivating bag, water outlet, softening, tiger can be considered Milk mushroom sclerotium is mature on the whole, because when the compost contraction in cultivating bag, water outlet, softening, the nutrition in cultivating bag disappears substantially It has been consumed that, Sclerotium of Pleurotus tuber regium would not continue to grow up because of nonnutritive offer.
Embodiment 2, method, the key step of a kind of indoor industrially production polyporus rhinoceros cooke mushroom include:
A, solid medium is prepared, its formulation weight is than component:Bagasse 15%;Wood chip 38%;Wheat bran 21%;Cotton seed hulls 23%;Sucrose 1.5%;Calcium carbonate 1.5%;After formula ratio component is fully mixed, the allotment that adds water moistens it, the amount typically added water Do not dripped with to hold culture medium and be advisable.
B, the making of cultivating bag, sterilizing and inoculation:Using 17 × 33cm polypropylene plastics pocket, load the training of step A solids Base is supported, after conventional sterilant, cooling is standby, and each cultivating bag then is connected into Pleurotus tuber-regium strain in sterile board, then moved on to Cultivated in 20-25 DEG C of greenhouse;
C, cultivation management I:After inoculation is survived, first check for strain and survive situation, if strain turns white, bacterium group restoration ecosystem, Other miscellaneous bacterias are not infected again, it is possible to which cultivating bag is emitted on the bedstead of cultivating chamber, indoor temperature is controlled at 25-35 DEG C Between, humidity 75-80%, after culture 30-40 days, pure white mycelia sends out purseful, then above compost or middle mycelia starts to collect Knot, forms Sclerotium of Pleurotus tuber regium, when sclerotium soon bursting polybag, can slough Plastibell, takes out tampon, unclamps sack, Prevent polybag from rupturing, after 4 months, a Sclerotium of Pleurotus tuber regium is can obtain in each cultivating bag;
D, cultivation management II, when Sclerotium of Pleurotus tuber regium is mature on the whole, bring up to 36-37 DEG C, humidity brings up to 90- by temperature After 92%, 1-2 days, Sclerotium of Pleurotus tuber regium surface grows the mushroom bud of white, when bud ratio >=90%, then temperature readjustment is arrived into 28-32 DEG C, humidity readjustment arrives 75-85%, then by the culture of 10-15 days, the polyporus rhinoceros cooke mushroom grown(As shown in Figure 2)It can just adopt Receive, it is finished product to dry after doing.The present embodiment, is sprouted by Sclerotium of Pleurotus tuber regium and grows the conversion ratio of polyporus rhinoceros cooke mushroom(Strain)Up to 70- 80%, polyporus rhinoceros cooke mushroom is abundant meat, dries and meets requirement of being used as medicine after doing.
Grown on Sclerotium of Pleurotus tuber regium after polyporus rhinoceros cooke mushroom, absorb the nutrition of whole Sclerotium of Pleurotus tuber regium, work as polyporus rhinoceros cooke When mushroom maturation can be harvested, the soft texture of Sclerotium of Pleurotus tuber regium, without value, is used as garbage disposal.
A kind of method that embodiment 3, indoor industrially produce polyporus rhinoceros cooke mushroom.
The present embodiment is on the basis of the step A-C of embodiment 2, still to retain the temperature and humidity described in step C, i.e., after Continuous control temperature is between 25-35 DEG C, and humidity is 75-80%, continues to cultivate, 10-20 days, then there is a small amount of polyporus rhinoceros cooke mushroom Grow, sprout uneven, but bud ratio is less than 50%, and polyporus rhinoceros cooke mushroom was plucked after 15 days, and polyporus rhinoceros cooke mushroom yield is to implement The 25-30% of example 2.
A kind of method that embodiment 4, indoor industrially produce polyporus rhinoceros cooke mushroom.
The present embodiment is on the basis of the step A-C of embodiment 2, temperature to be brought up into 36-37 DEG C, humidity brings up to 90- After 92%, 1-2 days, Sclerotium of Pleurotus tuber regium surface grow white mushroom bud, and continue retain 36-37 DEG C temperature and 90-92% it is wet The cultivation management method of degree, polyporus rhinoceros cooke mushroom fast growth, then brave breast spirit can be just harvested comprehensively by the culture of 8-10 days Sesame mushroom, but polyporus rhinoceros cooke mushroom rotten to the corn rate up to more than 20%, and polyporus rhinoceros cooke mushroom soft texture, density is low, poor quality, tiger breast spirit Sesame mushroom yield is smaller than embodiment 2 on the contrary.

Claims (1)

1. a kind of method that indoor industrially produces polyporus rhinoceros cooke mushroom, key step includes:
A, solid medium is prepared, its formulation weight is than component:Bagasse 10-15%;Wood chip 35-45%;Wheat bran 20-30%;Cottonseed Shell 20-25%;Sucrose 1-3%;Calcium carbonate 1-2%;
B, the making of cultivating bag, sterilizing and inoculation:Using polybag, load step A solid mediums, it is cold after conventional sterilant It is standby, each cultivating bag is then connected into Pleurotus tuber-regium strain in sterile board, then moves on in 18-25 DEG C of greenhouse and cultivates;
C, cultivation management I:Inoculation survive after, cultivating bag is emitted on the bedstead of cultivating chamber, temperature control 20-35 DEG C it Between, humidity 75-80%, after culture 30-40 days, pure white mycelia sends out purseful, then above compost or middle mycelia starts to collect Knot, forms Sclerotium of Pleurotus tuber regium, when sclerotium soon bursting polybag, can slough Plastibell, takes out tampon, unclamps sack, Prevent polybag from rupturing, after 4 months, a Sclerotium of Pleurotus tuber regium is can obtain in each cultivating bag;
D, cultivation management II:When Sclerotium of Pleurotus tuber regium is mature on the whole, temperature is brought up to 36-37 DEG C, humidity brings up to 90-92%, After 1-2 days, Sclerotium of Pleurotus tuber regium surface grows the mushroom bud of white, when bud ratio >=90%, then temperature readjustment arrived into 28-32 DEG C, wet Degree readjustment arrives 75-85%, then by the culture of 10-15 days, the polyporus rhinoceros cooke mushroom grown can just harvest, dry it is dry after for finished product.
CN201510678695.3A 2015-10-20 2015-10-20 The method that indoor industrially produces polyporus rhinoceros cooke mushroom Active CN105165409B (en)

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CN105557303A (en) * 2015-12-16 2016-05-11 新汇生物科技(梅州)有限公司 Indoor precise cultivation method of pleurotus tuber regium
CN107434547A (en) * 2016-05-26 2017-12-05 桂林宏泰养殖农民专业合作社 The Pleurotus tuber-regium high-yield culturing material made with multiple kinds of crops discarded object
CN110024618B (en) * 2019-05-22 2021-02-12 江苏康淼源生物科技有限公司 Cut-log cultivation method of polyporusrhinocerus cooke
CN114365657A (en) * 2021-12-27 2022-04-19 华中农业大学 Indoor potting method for sclerotium of Pleurotus tuber-regium

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CN103168617A (en) * 2013-01-14 2013-06-26 杭州益禾农业科技有限公司 Cut-log cultivation method of polyporusrhinocerus cooke

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CN1568653A (en) * 2004-05-13 2005-01-26 温宝国 Factory production method for Pleurotus tuber- regium (Fr.)Sing and relevant culture medium thereof
CN102301915A (en) * 2011-07-19 2012-01-04 抚州市临川区金山生物科技发展中心 Method for earthed cultivation and strain production of Pleurotus tuberregium and culture medium
CN103168617A (en) * 2013-01-14 2013-06-26 杭州益禾农业科技有限公司 Cut-log cultivation method of polyporusrhinocerus cooke

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