CN107794226A - A kind of Wild ganoderma strain selection - Google Patents

Abstract

The invention discloses a kind of Wild ganoderma strain selection, comprise the following steps：1）Parent species seed selection；2）Original seed seed selection；3）Cultigen seed selection etc..Invention passes through parent species seed selection, original seed seed selection, cultigen seed selection, Wild ganoderma is tamed into the strain that can carry out artificial cultivation, its mycelia is pure white sturdy, and vitality is vigorous, anti- miscellaneous bacteria is strong, the ganoderma lucidum cultivated, its cap is abundant hard, and quality is superior, effective ingredient is high, go out sesame year limit for length, it is good in economic efficiency, there is vast potential for future development.

Description

A kind of Wild ganoderma strain selection

Technical field

The present invention relates to a kind of ganoderma lucidum cultivation method, especially a kind of Wild ganoderma strain selection.

Background technology

Ganoderma lucidum is born on the base portion or rotten wood of broad leaf tree, on the rotten scar of trees, is also born in sometimes on coniferous tree,《Guiding principle Mesh》Spring is other.

Ganodermataceae is an important section for higher fungus, is that the world is extensively distributed monoid.Wild ganoderma, it is phase as its name suggests For propagating ganoderma lucidum artificially, the pure ganoderma lucidum grown naturally in forest is referred to.Wild ganoderma can be used for treating acute virus The diseases such as property hepatitis, chronic bronchitis, bronchial astehma and arrhythmia cordis.

All after even continuous cropping is ploughed, kind will be degenerated in general crops, and virosis is easily sent out, and again be difficult to plantation development phase Same kind！There is the problems such as serious variety deterioration and Quality Down for strain used in Chinese ganoderma lucidum industry.Quality compared with High lucidum seed is introduced by from Japan, South Korea mostly, and can equally degenerated after the several years.

Wild ganoderma is bred by basidiospore, and Reishi sporule has four kinds of sexes：A1, a2, b1, b2, the only class of a, b two are not The other spore mating of the same sex can just grow fructification, and this is the embodiment of the Nature survival of the fittest rule, also ensure that high-quality ganoderma lucidum The procreation of strain.And Ganoderma Lucidum uses vegetative propagation mostly, i.e., strain is cultivated by mycelial division, then mycelia Body is split, and cultivates more strains again, does not need mating process therebetween.Vegetative propagation generation upon generation of is gone down, just as the mankind are near Parent gets married equally, necessarily causes the degeneration of strain.

The content of the invention

The present invention is intended to provide a kind of Wild ganoderma strain selection, comprises the following steps：

1）Parent species seed selection

A type of selection is complete, cap is abundant, stem is long, not lopsided, disease resistance is strong, the Wild ganoderma fructification of firm parachute-opening, by it Stem base portion is cut off, and is fetched with aseptic water washing several times, is then put kind into and is connect in case and steep 4-7 minutes only with 75% alcohol, with sterilization Cotton is cleaned 3-5 times, then with aseptic water washing, after with scalpel prune fructification cot, its bacterial context is cut into the big granule of soya bean, Through in flame disinfection transposing to mother culture media test tube slant；

2）Potato is first cleaned into peeling, then weighs 200 grams and is cut into small pieces, adds boiling to boil 20-30 minutes, with multilayer gauze mistake Filter, heating, then add 18-20 grams of agar, continue heating stirring and mix, after agar has dissolved, add 20 grams of glucose, 3 grams of phosphorus Acid dihydride potassium, 1 gram of epsom salt, stirs, and supplies moisture again to 1000 milliliters after slightly cooling down, mixes 20% again afterwards Ganoderma lucidum fruitbody cooks the filtered fluid of liquid, dispenses test tube or conical flask, jumps a queue, wraps up, and 100-115 DEG C of sterilizing takes after 20 minutes Go out test tube pendulum inclined-plane, store standby after cooling, the test tube for accessing Wild ganoderma is placed in 27 DEG C of insulating boxs and cultivated 6-8 days, is chosen Select pollution-free, walk that bacterium speed is fast, and mycelia is pure white sturdy, clearly inclined-plane, picking mycelia front end part carry out expander for growth, then It is placed in 4 DEG C of refrigerators and preserves, as lucid ganoderma stock culture；

3）Original seed seed selection

The lucid ganoderma stock culture preserved in refrigerator is taken out, after recovering 1-2 days at normal temperatures, carries out expander, then accesses Primary spawn Base, its culture medium prescription are：Weed tree sawdust 70-80 parts, wheat bran 10-15 parts, rice sugar 4-7 parts, analysis for soybean powder 1-3 parts, 2 parts of land plaster, PH5-6, water content 50-60%, rearmounted 27 DEG C of culturing room in cultivate, incubation time about 25-32 days, produce ganoderma lucidum original seed；

4）Cultigen seed selection

Ganoderma lucidum original seed is accessed into Cultivar culture medium, its culture medium prescription is weed tree sawdust 70-80 parts, wheat bran 10-15 parts, rice sugar 4- 7 parts, analysis for soybean powder 1-3 parts, 2 parts, PH5-6, water content 50-60% of land plaster, rearmounted 27 DEG C of culturing room in cultivate, incubation time About 25-32 days, full bottle can be covered with, choose the pure white sturdy, strain that viability is strong of mycelia as cultivation of glossy ganoderma kind.

The Wild ganoderma strain cultivated using the above method, mycelia is pure white sturdy, and vitality is vigorous, and anti-miscellaneous bacteria is strong, cultivation The ganoderma lucidum gone out, its cap is abundant hard, and quality is superior, and effective ingredient is high, goes out sesame year limit for length, good in economic efficiency, has wide Wealthy development prospect.

Embodiment

Implement ground feux rouges village of Taijiang County desk tray township

1）Parent species seed selection

A type of selection is complete, cap is abundant, stem is long, not lopsided, disease resistance is strong, the Wild ganoderma fructification of firm parachute-opening, by it Stem base portion is cut off, and is fetched with aseptic water washing several times, is then put kind into and is connect in case and steeped 5 minutes only with 75% alcohol, uses aseptic cotton Flower is cleaned 4 times, then with aseptic water washing, after with scalpel prune fructification cot, its bacterial context is cut into the big granule of soya bean, through fire Flame sterilizes transposing to mother culture media test tube slant；

Master clock culture medium compound method is that potato first is cleaned into peeling, then weighs 200 grams and be cut into small pieces, and adds boiling to boil 30 points Clock, with 8 layers of filtered through gauze, heat, then add 20 grams of agar, continue heating stirring and mix, after agar has dissolved, add 20 grams of Portugals Grape sugar, 3 grams of potassium dihydrogen phosphates：KH2PO4,1 grams of epsom salt：MgSO47H2O, stir, water is supplied again after slightly cooling down Point to 1000 milliliters, the ganoderma lucidum fruitbody for mixing 20% again afterwards cooks the filtered fluid of liquid, dispenses test tube or conical flask, jump a queue, Wrapping, 100-115 DEG C sterilizing 20 minutes after take out test tube pendulum inclined-plane, stored after cooling it is standby, will access Wild ganoderma test tube It is placed in 27 DEG C of insulating boxs and cultivates 8 days, select pollution-free, walk that bacterium speed is fast, and mycelia is pure white sturdy, growth clearly inclined-plane, chooses Take mycelia front end part to carry out expander, be subsequently placed in 4 DEG C of refrigerators and preserve, as lucid ganoderma stock culture；

2）Original seed seed selection

The lucid ganoderma stock culture preserved in refrigerator is taken out, after recovering 2 days at normal temperatures, carries out expander, then accesses pedigree seed culture medium, Its culture medium prescription is：80 parts of weed tree sawdust, 10 parts of wheat bran, 6 parts of rice sugar, 2 parts of analysis for soybean powder, 2 parts, PH5-6 of land plaster, water content 50-60%, rearmounted 27 DEG C of culturing room in cultivate, incubation time about 25-32 days, produce ganoderma lucidum original seed；

3）Cultigen seed selection

Ganoderma lucidum original seed is accessed into Cultivar culture medium, its culture medium prescription is：80 parts of weed tree sawdust, 10 parts of wheat bran, 6 parts of rice sugar are yellow 2 parts of bean powder, 2 parts, PH5-6, water content 50-60% of land plaster, rearmounted 27 DEG C of culturing room in cultivate, incubation time 25-32 days, It can cover with full bottle, choose the pure white sturdy, strain that viability is strong of mycelia as cultivation of glossy ganoderma kind.

Claims (1)

1. a kind of Wild ganoderma strain selection, it is characterised in that comprise the following steps：

1）Parent species seed selection

A type of selection is complete, cap is abundant, stem is long, not lopsided, disease resistance is strong, the Wild ganoderma fructification of firm parachute-opening, by it Stem base portion is cut off, and is fetched with aseptic water washing several times, is then put kind into and is connect in case and steep 4-7 minutes only with 75% alcohol, with sterilization Cotton is cleaned 3-5 times, then with aseptic water washing, after with scalpel prune fructification cot, its bacterial context is cut into the big granule of soya bean, Through in flame disinfection transposing to mother culture media test tube slant；

2）Potato is first cleaned into peeling, then weighs 200 grams and is cut into small pieces, adds boiling to boil 20-30 minutes, with multilayer gauze mistake Filter, heating, then add 18-20 grams of agar, continue heating stirring and mix, after agar has dissolved, add 20 grams of glucose, 3 grams of phosphorus Acid dihydride potassium, 1 gram of epsom salt, stirs, and supplies moisture again to 1000 milliliters after slightly cooling down, mixes 20% again afterwards Ganoderma lucidum fruitbody cooks the filtered fluid of liquid, dispenses test tube or conical flask, jumps a queue, wraps up, and 100-115 DEG C of sterilizing takes after 20 minutes Go out test tube pendulum inclined-plane, store standby after cooling, the test tube for accessing Wild ganoderma is placed in 27 DEG C of insulating boxs and cultivated 6-8 days, is chosen Select pollution-free, walk that bacterium speed is fast, and mycelia is pure white sturdy, clearly inclined-plane, picking mycelia front end part carry out expander for growth, then It is placed in 4 DEG C of refrigerators and preserves, as lucid ganoderma stock culture；

3）Original seed seed selection

The lucid ganoderma stock culture preserved in refrigerator is taken out, after recovering 1-2 days at normal temperatures, carries out expander, then accesses Primary spawn Base, its culture medium prescription are：Weed tree sawdust 70-80 parts, wheat bran 10-15 parts, rice sugar 4-7 parts, analysis for soybean powder 1-3 parts, 2 parts of land plaster, PH5-6, water content 50-60%, rearmounted 27 DEG C of culturing room in cultivate, incubation time 25-32 days, produce ganoderma lucidum original seed；

4）Cultigen seed selection

Ganoderma lucidum original seed is accessed into Cultivar culture medium, its culture medium prescription is weed tree sawdust 70-80 parts, wheat bran 10-15 parts, rice sugar 4- 7 parts, analysis for soybean powder 1-3 parts, 2 parts, PH5-6, water content 50-60% of land plaster, rearmounted 27 DEG C of culturing room in cultivate, incubation time About 25-32 days, full bottle can be covered with, choose the pure white sturdy, strain that viability is strong of mycelia as cultivation of glossy ganoderma kind.