CN1338509A - Process for preparing seeds of edible and medical ectomycorrhize fungus and its antificial cuture method - Google Patents
Process for preparing seeds of edible and medical ectomycorrhize fungus and its antificial cuture method Download PDFInfo
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Abstract
A process for preparing seed of edible and medical ectomycorrhiza fungus and culturing it includes such steps as treating the fruiting body with aseptic antistaling liquid, taking the tissue blocks near the base of stipe, separating tissue, cultivating mother seed with PDA culture medium plus pine juice, cultivating elites with rice grains, making plastic cultivation bags, and cultivating by covering soil while supplementing water and nutritive liquid.
Description
The invention belongs to the edible medicinal fungus production of hybrid seeds and artificial cultivation technique field, relate in particular to edible medicinal ectomycorrhiza fungus preparing seeds and artificial cultivation method.
There are not artificial culture King Boletus (Boletus edulis), Delicious lactarius (Lactrius deliciosus), succulence breast mushroom (Lactarius volemus), the chanterelle (Cantharellus vibarius) of success, the method for green mushroom edible medicinal ectotrophic mycorrhizas such as (Russula virescens) at present.The finding report has: 1, Fang Pingyi " to the tame trial of Delicious lactarius " (" edible mushrooms " 1994 the 3rd phases).Its method that adopts is: (1) adopts the MS minimum medium, removes plant hormone and phytokinin, and additional 3 restrain peptones, 2 restrains yeast extracts, 20 gram agar as separating bacterial classification, the cultivation of mother's kind and preserving substratum; (2) pedigree seed culture medium adopts the mixture of wheat or cow dung powder and rice; (3) sporophore formation substratum is rotten cogongrass of cotton seed hull and sucrose; (4) the female kind method of separation and Culture is to choose the part cap of stem or stem attachment region, cultivates on slant medium, selects the mycelia switching and cultivates female the kind, expands original seed again, carries out sporophore at last and cultivates.Its experimental result is can form sporophore under the artificial cultivation condition, but is difficult for forming, and forms also to be difficult for growing up.2, invention " Delicious lactarius mother culture media and Delicious lactarius artificial cultivation method " (C1240572A) provides half artificial cultivation method, and used substratum mycelial growth is slow, and growth cycle is long, needs 4-5 from the production of hybrid seeds to the fruiting, need plant Pinus massoniana Lamb simultaneously.
The purpose of this invention is to provide a kind ofly develop that new bacterial strain is particularly eaten, the production of hybrid seeds of medicinal ectotrophic mycorrhiza and stable high yield artificial cultivation method, widen edible medicinal fungus artificial culture field, edible medicinal ectotrophic mycorrhizas such as realization King Boletus, Delicious lactarius, succulence breast mushroom are cultivated then can produce many batches of mushrooms then, cultivated fruiting for many years in 1 year.
The objective of the invention is to realize by the following technical solutions: the cultivation operation comprises the female kind-system original seed of the kind-system of getting-system cultivar-system cultivating bag-management of producing mushroom.Principal feature of the present invention is:
Gathering ground on-the-spot disposal sporophore with sterilized antistaling liquid when (1.1) collecting seed, separate tissue is got the big tissue block of stem base portion soya bean near shoestring in aseptic nontoxic environment, change on the mother culture media, in 22-32 ℃, cultivate, treat that tissue block sprouts the tube of purifying behind the mycelia;
(1.1.1) sterilized antistaling liquid making method, filling a prescription is 1000 milliliters of edible alum 10 grams, iodated salt 20 grams, red 30 grams of 3% furans, water, compound method is: after alum and furans pellet are added the water heating for dissolving respectively, filter, get filtrate mixing, with the bottling sterilization of salt fusion back, the time spent shakes up;
(1.1.2) food, medicinal ectotrophic mycorrhiza mother culture media compound method:
A. make pin tree sap: getting bright Pinus massoniana Lamb, not have the joint xylem be that raw material is cut or is ground into particle,
Add and change slow fire behind the water boil and boiled 2 hours, 1 kilogram of timber adds water and endures to 0.5 for 1 liter
Rise with filtered through gauze standby;
B. make potato juice: 0.25 kilogram of peeling potato is added 0.7 liter in water, endure with slow fire
To 0.5 liter of filtration, standby;
C. potassium primary phosphate 1.5 restrains, and sal epsom 1 gram is used the small amount of thermal water dissolution;
D. above-mentioned three kinds of solution are mixed, add agar 20 grams, sucrose 20 grams, boil dissolving
Back slow fire is boiled dry to 1000 milliliters, packing test tube, 10 milliliters of every pipes.The test tube cotton
The inner bag of plug micropore damp-proof membranes, 71 bundles bind back bag kraft paper then;
Must earlier freezing air in the pressure kettle be discharged when E. sterilizing, sterilization pressure is 1.2-1.5
Kilogram/square centimeter was sterilized 45 minutes, boiled after pressure returns 0, allowed waste heat dry by the fire
Dried tampon is put the inclined-plane in about 50 ℃ of temperature.Test tube need cover the guarantor up and down during the pendulum inclined-plane
Adiabator is treated vertically to allow water of condensation come back to tiltedly in test tube immediately after the inclined-plane solidifies
In the face;
(1.2) original seed, cultivar use paddy particle kind, and its preparation method is: the paddy of the chest that will germinate brokenly boils and completes with potato 20%, white sugar 2%, potassium primary phosphate 0.1%, sal epsom 0.05% mixed liquid, admixes 3% terra alba, with saline bottle packing sterilization; Cooling back inoculation is put under the 22-32 ℃ of temperature and is cultivated, and carries out shaking the first time bottle after three days, shakes single-trip container later every day and checks purity, is full of grain until mycelia, use the same method enlarged culturing 1 time of cultivar;
(1.3) making method of cultivating bag is:
(1.3.1) plant formulation is: 5 kilograms of 400 kilograms of the fresh wood chips of Pinus massoniana Lamb, boll hull double centner, wheat bran double centner, 10 kilograms of white sugar, 0.75 kilogram of potassium primary phosphate, 0.5 kilogram in sal epsom, Pinus massoniana Lamb forest land muck soils double centner, alum, water content 60%, the PH nature;
(1.3.2) culture material adopts sterilising method twice: after damping is mixed in the culture material mixing thoroughly, pack into rapidly in the 75cm*45cm Fibre Bag, the liftoff heap of building, seal with plastic film around the heap, if thermal insulation layer, thermal insulation layer adds by the double-deck close net of wall screw fixed, utilize the blower fan pressurization to make high pressure steam Rapid Cycle heating in vapour generator and culture material, culture material reached 55 ℃ at 1 hour, reached 70 ℃ in 2 hours, reached 85 ℃ in 3 hours, and kept 4 hours, through so pre-steaming, after dried wet uniform culture material turns over cold, the packing plastics bag, ten packedly go into a Fibre Bag; Carry out the steam sterilizing second time again, kept 100 ℃ of temperature 10 hours;
(1.3.3) inoculation method is: the inoculation before bacterial classification is shaken diffusing, the flame disinfection bottleneck, open plastics bag after, dig an aperture with the finger barrier film on the base-material face, bacterial classification is poured in the hole, squeezes culture material with the finger barrier film and covers bacterial classification completely, use flat ring set mouth again, use the bungee tying behind the sack reflexed, postvaccinal bacterium bag sack is inoculated on the spot on the spot and is cultivated down, culture temperature is 22-32 ℃, humidity is 70%, and mycelia is covered with culture material substantially, punching ventilation when temperature is stabilized in below 32 ℃;
(1.3.4) method of punching ventilation is: the bacterium tube that will cover with mycelia is played the hole ventilation of three to four thick fingers in the bottom of bacterium tube with special high temperature adjusting electric iron, bacterium tube after the punching is emitted on the end and is covered with on the clean river sand of plastic film, adhere to spray disinfectant insecticidal/acaricidal agent sooner or later, temperature remains on 25-32 ℃;
(1.3.5) the acaricidal compound method of disinfection: proportioning is 900 milliliters of 40% formaldehyde solutions, 50 milliliters of dehydrated alcohols, 20 milliliters in 40% Rogor, 20 milliliters of 80% SD-1750,10 milliliters of 20% kelthanes, compound method is that Rogor, SD-1750, kelthane are measured respectively, pour in the non-metallic container, add alcohol, shake up the back and add formaldehyde, fully shake up back bottling sealing, put shady and cool dry place and keep in Dark Place, the time spent shakes up;
(1.4) the management of producing mushroom mode is: the bacterium tube that will send out bacterium good takes off bag, is emitted on the fruiting place of sterilizing, and stays gap 4cm between every bag of bacterium tube, builds plastic film while arranging.Treat that the bacterium tube recovers wound and 4 lid fermentations of long mycelia time-division material soil and pine forest soil at every turn after 1-2 centimetre, spray the worm miticide behind the blinding, mycelia grows the soil table again case face plantation grass, treat that grass is long to two leaves spray form water wholeheartedly the time, adopt the epiphragma on daytime, cover non-woven fabrics evening, do not cover thing morning during 6-8, promote the formation and the growth of sporophore;
(1.4.1) make fermentation material soil: select field soil or dish soil to dry and sieve, put human excrement double centner, 20 kilograms of calcium superphosphate, Pinus massoniana Lamb trees bits double centner, 10 kilograms in alum, red 10 kilograms of furans for per 1000 kilograms, mix water transfer thoroughly to water content 60%, pile rectangular, the loam cake overlay, utilize solar irradiation heating fermentation two weeks, build heap back and carried out turning for the first time on the 7th day to the vacant lot of disinfection in advance, the disinfection miticide of surface sprinkling 5%, get through pore to expecting the bottom plastic film, to expect native secondary turning, dry standby after 15 days in the 10th day;
(1.4.2) fruiting place sterilization: the vacant lot in the good seat of gather water Northern Dynasties south, build the cloudy fluffy waterways of holding successfully, after temperature was stabilized in below 35 ℃, place FCL spray disinfectant insecticidal/acaricidal agent, epiphragma carried out the place sterilization in 48 hours.
Food, medicinal external bacterium enjoy people to pay close attention to bacterium because of its unique local flavor and medicinal function, but always can not mass production, and the present invention has that production technique is simple, output is high, the advantage of superior product quality, and has solved the technical problem of artificial batch process.
With the Delicious lactarius artificial culture is that embodiment elaborates: the Delicious lactarius artificial culture technological process of production is the female kind-system original seed of the kind-system of getting-system cultivar-system cultivating bag-management of producing mushroom.
Get and prepare sterilized antistaling liquid when planting and making female the kind earlier, prescription is: 1000 milliliters of edible alum 10 grams, iodated salt 20 grams, red 3 grams of 3% furans, water, compound method is: after alum and furans pellet are added the water heating for dissolving respectively, filter, get filtrate mixing, with the bottling sterilization of salt fusion back, the time spent shakes up;
Do mother culture media, its compound method:
A. make pin tree sap: getting bright Pinus massoniana Lamb, not have the joint xylem be that raw material is cut or is ground into particle,
Add and change slow fire behind the water boil and boiled 2 hours, 1 kilogram of timber adds water and endures to 0.5 for 1 liter
Rise and use filtered through gauze, standby;
B. make potato juice: with 0.25 kilogram the peeling potato add 0.7 liter in water, with slow fire endure to
Filter 0.5 rise, standby;
C. potassium primary phosphate 1.5 restrains, and sal epsom 1 gram is used the small amount of thermal water dissolution;
D. above-mentioned three kinds of solution are mixed, add agar 20 grams, sucrose 20 grams, boil dissolving
Back slow fire is boiled dry to 1000 milliliters, packing test tube, 10 milliliters of every pipes.The test tube cotton
The inner bag of plug micropore damp-proof membranes, 71 bundles bind back bag kraft paper then;
Must earlier freezing air in the pressure kettle be discharged when E. sterilizing, sterilization pressure is 1.2-1.5
Kilogram/square centimeter was sterilized 45 minutes, boiled after pressure returns 0, allowed waste heat dry by the fire
Dried tampon is put the inclined-plane in about 50 degree temperature.Test tube need cover the guarantor up and down during the pendulum inclined-plane
Adiabator is treated vertically to allow water of condensation come back to tiltedly in test tube immediately after the inclined-plane solidifies
In the face;
Gathering ground on-the-spot disposal sporophore with sterilized antistaling liquid during seed collecting, separate tissue is got the big tissue block of stem base portion soya bean near shoestring in aseptic nontoxic environment, change on the mother culture media, cultivates in 22-32 ℃.Treat that tissue block sprouts the tube of purifying behind the mycelia;
Original seed, cultivar use paddy particle kind, and its preparation method is: the paddy of the chest that will germinate brokenly boils and completes with potato 20%, white sugar 2%, potassium primary phosphate 0.1%, sal epsom 0.05% mixed solution, admixes 3% terra alba, with saline bottle packing sterilization; Cooling back inoculation is put under the 22-32 ℃ of temperature and is cultivated, and carries out shaking the first time bottle after three days, shakes single-trip container later every day and checks purity, is full of grain until mycelia, use the same method enlarged culturing 1 time of cultivar;
The making method of cultivating bag is:
Cultivation material: get 5 kilograms of 400 kilograms of the fresh wood chips of Pinus massoniana Lamb, boll hull double centner, wheat bran double centner, 10 kilograms of white sugar, 0.75 kilogram of potassium primary phosphate, 0.5 kilogram in sal epsom, Pinus massoniana Lamb forest land muck soils double centner, alum, water content 60%, the PH nature;
Preparation disinfection miticide: proportioning is 900 milliliters of 40% formaldehyde solutions, 50 milliliters of dehydrated alcohols, 20 milliliters in 40% Rogor, 20 milliliters of 80% SD-1750,10 milliliters of 20% kelthanes, compound method is that Rogor, SD-1750, kelthane are measured respectively, pour in the non-metallic container, add alcohol, shake up the back and add formaldehyde, fully shake up back bottling sealing, put shady and cool dry place and keep in Dark Place, the time spent shakes up.
Culture material adopts sterilising method twice.After damping is mixed in the culture material mixing thoroughly, pack into rapidly in the 75cm*45cm Fibre Bag, the liftoff heap of building, seal with plastic film around the heap, if thermal insulation layer, thermal insulation layer adds by the double-deck close net of wall screw fixed, utilize the blower fan pressurization to make high pressure steam Rapid Cycle heating in vapour generator and culture material, culture material reached 55 ℃ at 1 hour, reached 70 ℃ in 2 hours, reached 85 ℃ in 3 hours, and kept 4 hours, through so pre-steaming, after dried wet uniform culture material turns over cold, the packing plastics bag, ten packedly go into a Fibre Bag; Carry out the steam sterilizing second time again, kept 100 ℃ of temperature 10 hours; Inoculation method is: before the inoculation bacterial classification is shaken diffusing, the flame disinfection bottleneck, after opening plastics bag, dig an aperture with the finger barrier film on the base-material face, bacterial classification is poured in the hole, squeezes culture material with the finger barrier film and covers bacterial classification completely, use flat ring set mouth again, use the bungee tying behind the sack reflexed, postvaccinal bacterium bag sack is inoculated on the spot on the spot and is cultivated down.Culture temperature is 22-32 ℃, and humidity is 70%, and mycelia is covered with culture material substantially, punching ventilation when temperature is stabilized in below 32 ℃;
The method of punching ventilation is: the bacterium tube that will cover with mycelia is played the hole ventilation of three to four thick fingers in the bottom of bacterium tube with special high temperature adjusting electric iron, bacterium tube after the punching is emitted on the end and is covered with on the clean river sand of plastic film, adhere to spray disinfectant insecticidal/acaricidal agent sooner or later, temperature remains on 25-32 ℃.
Management of producing mushroom:
1. make fermentation and expect that soil selects field soil or dish soil to dry and sieves, per 1000 kilograms of soil are put human excrement double centner, 20 kilograms of calcium superphosphate, Pinus massoniana Lamb trees bits double centner, 10 kilograms in alum, red 10 kilograms of furans, mix water transfer thoroughly to water content 60%, pile rectangular, the loam cake overlay, utilize solar irradiation heating fermentation two weeks, build heap back and carried out turning for the first time on the 7th day to the vacant lot of disinfection in advance, the disinfection miticide of surface sprinkling 5%, get through pore to expecting the end, the lid plastic film will be expected native secondary turning, dry standby after 15 days on the 10th day;
2. the vacant lot in the good seat of fruiting place sterilization gather water Northern Dynasties south is built cloudy fluffyly, holds the waterways successfully, temperature be stabilized in 35 degree following after, place FCL spray disinfectant insecticidal/acaricidal agent, epiphragma carried out the place sterilization in 48 hours;
3. after soil fruiting sends out bacterium good the bacterium tube is taken off bag, be emitted on the fruiting place of sterilizing, stay gap 4cm between every bag of bacterium tube, build plastic film while arranging.Treat that the bacterium tube lifts the material soil 2cm of membrane cover fermentation damping when recovering wound and long mycelia, the disinfection miticide of soil face spray 5%, epiphragma, when mycelia grows the soil table once more, lift following each 50% the composite soil 2cm of new soil of membrane cover one deck fermentation material soil and arable layer, the disinfection miticide of spray 3%, epiphragma, when the case face length expires mycelia, lift film, lid broad bean size does not contain the pine forest soil 2cm of humic, soil moisture content is 20%, spray 2% disinfection miticide again, epiphragma is cultivated mycelia, and the mycelia that enters this layer soil is sturdy dense white, when treating that mycelia length arrives near the soil table, the grass grass-seed that case face plantation sterilization is germinateed, the pine forest soil 1cm of lid broad bean size, humidity is controlled at 60%, and sprays 1% disinfection miticide, epiphragma again, when mycelia appears in native face, use the bamboo chip of sterilizing with plastic film frame height, and spray 0.5% urea, 0.15% potassium primary phosphate, 0.1% sal epsom nutritive medium promotes the grass growth, treat that grass is long to two leaves spray form water wholeheartedly the time, adopt the epiphragma on daytime, cover non-woven fabrics evening, do not cover thing morning during 6-8, create the condition of favourable fruiting, promote the formation and the growth of sporophore.Grass grows to four leaves wholeheartedly the time, cuts sheath and handles, because institute's nutritive medium of executing has promoter action, bed surface can grow green alga very soon to the phycophyta growth, can cut grass rare this moment.Ectotrophic mycorrhiza, particularly newborn mushroom ectotrophic mycorrhiza, mycelia is crisp easily broken, and fruiting requires height and constant humidity.Earthing and plant grass or green alga hinders mycelia when avoiding spraying water during fruiting, and regulate humidity.
Claims (1)
1. a food, medicinal ectomycorrhiza fungus preparing seeds and artificial cultivation method adopt conventional edible mushrooms artificial culture operation promptly to get the female kind-system original seed of kind-system-system cultivar-system cultivating bag-management of producing mushroom, and feature of the present invention is:
Gathering ground on-the-spot disposal sporophore with sterilized antistaling liquid when (1.1) collecting seed, separate tissue is got the big tissue block of stem base portion soya bean near shoestring in aseptic nontoxic environment, change on the mother culture media, in 22-32 ℃, cultivate, treat that tissue block sprouts the tube of purifying behind the mycelia;
(1.1.1) sterilized antistaling liquid making method, filling a prescription is 1000 milliliters of edible alum 10 grams, iodated salt 20 grams, red 30 grams of 3% furans, water, compound method is: after alum and furans pellet are added the water heating for dissolving respectively, filter, get filtrate mixing, with the bottling sterilization of salt fusion back, the time spent shakes up;
(1.1.2) food, medicinal ectotrophic mycorrhiza mother culture media compound method:
A. make pin tree sap: getting bright Pinus massoniana Lamb, not have the joint xylem be that raw material is cut or is ground into particle,
Add and change slow fire behind the water boil and boiled 2 hours, 1 kilogram of timber adds water and endures to 0.5 for 1 liter
Rise with filtered through gauze standby;
B. make potato juice: 0.25 kilogram of peeling potato is added 0.7 liter in water, endure to 0.5 with slow fire
Rise, filter, standby;
C. potassium primary phosphate 1.5 restrains, and sal epsom 1 gram is used the small amount of thermal water dissolution;
D. above-mentioned three kinds of solution are mixed, add agar 20 grams, sucrose 20 grams, boil dissolving
Back slow fire is boiled dry to 1000 milliliters, packing test tube, 10 milliliters of every pipes.The test tube cotton
The inner bag of plug micropore damp-proof membranes, 71 bundles bind back bag kraft paper then;
Must earlier freezing air in the pressure kettle be discharged when E. sterilizing, sterilization pressure is 1.2-1.5
Kilogram/square centimeter was sterilized 45 minutes, boiled after pressure returns 0, allowed waste heat dry by the fire
Dried tampon is put the inclined-plane in about 50 ℃ of temperature.Test tube need cover the guarantor up and down during the pendulum inclined-plane
Adiabator is treated vertically to allow water of condensation come back to tiltedly in test tube immediately after the inclined-plane solidifies
In the face;
(1.2) original seed, cultivar use paddy particle kind, its preparation method is: the paddy of the chest that will germinate brokenly boils and completes with potato 20%, white sugar 2%, potassium primary phosphate 0.1%, sal epsom 0.05% mixed solution, admix 3% terra alba, with saline bottle packing sterilization; Cooling back inoculation is put under the 22-32 ℃ of temperature and is cultivated, and carries out shaking the first time bottle after three days, shakes single-trip container later every day and checks purity, is full of grain until mycelia, use the same method enlarged culturing 1 time of cultivar;
(1.3) making method of cultivating bag is:
(1.3.1) plant formulation is: 5 kilograms of 400 kilograms of the fresh wood chips of Pinus massoniana Lamb, boll hull double centner, wheat bran double centner, 10 kilograms of white sugar, 0.75 kilogram of potassium primary phosphate, 0.5 kilogram in sal epsom, Pinus massoniana Lamb forest land muck soils double centner, alum, water content 60%, the PH nature;
(1.3.2) culture material adopts sterilising method twice: after damping is mixed in the culture material mixing thoroughly, pack into rapidly in the 75cm*45cm Fibre Bag, the liftoff heap of building, seal with plastic film around the heap, if thermal insulation layer, thermal insulation layer adds by the double-deck close net of wall screw fixed, utilize the blower fan pressurization to make high pressure steam Rapid Cycle heating in vapour generator and culture material, culture material reached 55 ℃ at 1 hour, reached 70 ℃ in 2 hours, reached 85 ℃ in 3 hours, and kept 4 hours, through so pre-steaming, after dried wet uniform culture material turns over cold, the packing plastics bag, ten packedly go into a Fibre Bag; Carry out the steam sterilizing second time again, kept 100 ℃ of temperature 10 hours;
(1.3.3) inoculation method is: before the inoculation bacterial classification is shaken diffusing, the flame disinfection bottleneck, after opening plastics bag, dig an aperture with the finger barrier film on the base-material face, bacterial classification is poured in the hole, squeezes culture material with the finger barrier film and covers bacterial classification completely, use flat ring set mouth again, use the bungee tying behind the sack reflexed, postvaccinal bacterium bag sack is inoculated on the spot on the spot and is cultivated down.Culture temperature is 22-32 ℃, and humidity is 70%, and mycelia is covered with culture material substantially, punching ventilation when temperature is stabilized in below 32 ℃;
(1.3.4) method of punching ventilation is: the bacterium tube that will cover with mycelia is played the hole ventilation of three to four thick fingers in the bottom of bacterium tube with special high temperature adjusting electric iron, bacterium tube after the punching is emitted on the end and is covered with on the clean river sand of plastic film, adhere to spray disinfectant insecticidal/acaricidal agent sooner or later, temperature remains on 25-32 ℃;
(1.3.5) the acaricidal compound method of disinfection: proportioning is 900 milliliters of 40% formaldehyde solutions, 50 milliliters of dehydrated alcohols, 20 milliliters in 40% Rogor, 20 milliliters of 80% SD-1750,10 milliliters of 20% kelthanes, compound method is that Rogor, SD-1750, kelthane are measured respectively, pour in the non-metallic container, add alcohol, shake up the back and add formaldehyde, fully shake up back bottling sealing, put shady and cool dry place and keep in Dark Place, the time spent shakes up;
(1.4) the management of producing mushroom mode is; The bacterium tube of sending out bacterium good is taken off bag, be emitted on the fruiting place of sterilizing, stay gap 4cm between every bag of bacterium tube, build plastic film while arranging.Treat that the bacterium tube recovers wound and 4 lid fermentations of long mycelia time-division material soil and pine forest soil at every turn after 1-2 centimetre, spray mite and insect killing agent behind the blinding, mycelia grows the soil table again case face plantation grass, treat that grass is long to two leaves spray form water wholeheartedly the time, adopt the epiphragma on daytime, cover non-woven fabrics evening, do not cover thing morning during 6-8, promote the formation and the growth of sporophore;
(1.4.1) make fermentation material soil.Selecting field soil or dish soil to dry sieves, put human excrement double centner, 20 kilograms of calcium superphosphate, Pinus massoniana Lamb trees bits double centner, 10 kilograms in alum, red 10 kilograms of furans for per 1000 kilograms, mix water transfer thoroughly to water content 60%, pile rectangular, the loam cake overlay, utilize solar irradiation heating fermentation two weeks, build heap back and carried out turning for the first time on the 7th day to the vacant lot of disinfection in advance, the disinfection miticide of surface sprinkling 5%, get through pore to expecting the end, the lid plastic film will be expected native secondary turning, dry standby after 15 days on the 10th day;
(1.4.2) fruiting place sterilization, the cloudy fluffy waterways of holding successfully is built in the vacant lot in the good seat of gather water Northern Dynasties south, and after temperature was stabilized in below 35 ℃, place FCL spray disinfectant insecticidal/acaricidal agent, epiphragma carried out the place sterilization in 48 hours.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1301640C (en) * | 2003-12-02 | 2007-02-28 | 任岛峰 | Process for production of edible mushroom raw material bag growth |
| CN101690454B (en) * | 2009-09-29 | 2011-02-02 | 福建省农业科学院食用菌研究所 | Russula vinosa Lindbl yield increasing fungi and using method thereof |
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN103250564A (en) * | 2013-05-10 | 2013-08-21 | 西北农林科技大学 | Artificial cultivating method for chestnut mycorrhiza fungi |
| CN103283481A (en) * | 2012-03-02 | 2013-09-11 | 贵州省生物研究所 | Infecting method of pinus massoniana seedling by cantharellus cibarius |
| CN105027970A (en) * | 2015-07-06 | 2015-11-11 | 合肥福泉现代农业科技有限公司 | Pleurotus tuber-regium culture medium rich in selenium and germanium and preparing method thereof |
| CN106258484A (en) * | 2016-08-11 | 2017-01-04 | 甘肃民族师范学院 | A kind of high altitudes and cold area efficient implantation methods of Delicious lactarius |
| CN106754400A (en) * | 2016-11-22 | 2017-05-31 | 曹晓龙 | High mountain lucidum strain preparation method |
| CN112772298A (en) * | 2021-03-27 | 2021-05-11 | 云南林业职业技术学院 | Isolation culture medium for boletus brevicaulis strain |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301640C (en) * | 2003-12-02 | 2007-02-28 | 任岛峰 | Process for production of edible mushroom raw material bag growth |
| CN101690454B (en) * | 2009-09-29 | 2011-02-02 | 福建省农业科学院食用菌研究所 | Russula vinosa Lindbl yield increasing fungi and using method thereof |
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN103283481A (en) * | 2012-03-02 | 2013-09-11 | 贵州省生物研究所 | Infecting method of pinus massoniana seedling by cantharellus cibarius |
| CN103283481B (en) * | 2012-03-02 | 2015-08-26 | 贵州省生物研究所 | A kind of pixie stool is to the exhaust processes of Pinus Massoniana Young Seedlings |
| CN103250564A (en) * | 2013-05-10 | 2013-08-21 | 西北农林科技大学 | Artificial cultivating method for chestnut mycorrhiza fungi |
| CN103250564B (en) * | 2013-05-10 | 2015-01-07 | 西北农林科技大学 | Artificial cultivating method for chestnut mycorrhiza fungi |
| CN105027970A (en) * | 2015-07-06 | 2015-11-11 | 合肥福泉现代农业科技有限公司 | Pleurotus tuber-regium culture medium rich in selenium and germanium and preparing method thereof |
| CN106258484A (en) * | 2016-08-11 | 2017-01-04 | 甘肃民族师范学院 | A kind of high altitudes and cold area efficient implantation methods of Delicious lactarius |
| CN106754400A (en) * | 2016-11-22 | 2017-05-31 | 曹晓龙 | High mountain lucidum strain preparation method |
| CN112772298A (en) * | 2021-03-27 | 2021-05-11 | 云南林业职业技术学院 | Isolation culture medium for boletus brevicaulis strain |
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