CN103125274B - Grafting method between different types of ganoderma lucidum and potted landscape process - Google Patents
Grafting method between different types of ganoderma lucidum and potted landscape process Download PDFInfo
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- CN103125274B CN103125274B CN201310082690.5A CN201310082690A CN103125274B CN 103125274 B CN103125274 B CN 103125274B CN 201310082690 A CN201310082690 A CN 201310082690A CN 103125274 B CN103125274 B CN 103125274B
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Abstract
The invention relates to a grafting method between different types of ganoderma lucidum and a potted landscape process, and belongs to the field of fungus industries. Fungus balls of staghorn fungus are attached to a grafting connection point. After a scion is fixed in grafting, under a suitable environment condition, and after germination of the fungus balls, the connection point and the scion are rapidly and permanently fixed. Grafting points of the ganoderma lucidum are not restricted by growing points, and scions of the ganoderma lucidum are not restricted by the types or fungus strains of connection points. Manufacturing of various kinds of potted landscape is benefited, and strong advantages are showed through application of a ganoderma lucidum living body potted landscape process. In the growth stage of secondary ganoderma lucidum living bodies, different special kinds of the ganoderma lucidum are combined to grow integrally through the adoption of a manual grafting method, colorful and graceful kinds of the ganoderma lucidum potted landscape are manufactured, the ganoderma lucidum potted landscape is plain and fresh and attractive in scene, an implied meaning of auspicious stands out, cultural life of people is enriched, and important significance is provided for further improvement of economic benefits and social benefits of ganoderma lucidum production.
Description
Technical field
The invention belongs to bacterium industry field, be specifically related to a kind of different heteroplastic graft methods of glossy ganoderma and potted landscape technique.
Background technology
Glossy ganoderma is Basidiomycetes Polyporaceae Ganoderma fungi, and its kind has red sesame, purple sesame and Ganoderma lucidum etc., and glossy ganoderma is medicinal has strengthening the body resistance to consolidate the constitution, cure ten thousand diseases, its function is confirmed, the well-informed magical effect, is the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, possesses very high medical value.Glossy ganoderma is as potted landscape, and simple and unsophisticated pure and fresh, scene is attractive in appearance, outstanding lucky implied meaning, and exquisite lovely, the noble quality that also fully shows glossy ganoderma dish garden is gorgeous.Glossy ganoderma dish garden, with its unique classical design and deep artistic connotation, has become the first-selection of decorateeing high-grade room, office, hotel, hotel, is also the fashion good merchantable brand of present, collection.Produce glossy ganoderma dish garden and there is high economic benefit and social benefit.
Summary of the invention
The object of the present invention is to provide a kind of different heteroplastic graft methods of glossy ganoderma and potted landscape technique.
For achieving the above object, the technical solution used in the present invention is as follows:
First the present invention provides a kind of glossy ganoderma different heteroplastic graft methods, comprises the following steps:
(1) cultivating in bag Ganoderma lucidum, obtains deer horn shape fruit body, and obtains bacterium ball by Ganoderma lucidum liquid culture;
(2) other kind glossy ganoderma of cultivating in bag, obtains mature sporophore, and its cap is kidney shape, semicircle or subcircular, using this fruit body as scion, and along base portion truncation, gets off with sterile blade;
(3) the different heteroplastic graft methods of glossy ganoderma:
A, grafting environment: require surrounding air relative moisture to reach 85%～90%, 26～28 ℃ of temperature, illumination every day 12 hours, intensity of illumination 50 lx;
B, grafting: take deer horn shape sporophore growth point or away from growing point place as grafting point, on grafting point, first with sharp blade, epidermis is put down and cut 1 cutter, bacterium ball and scion are put to grafting point above, and with the fixing scion of bamboo let and grafting point, and it is stuffy to cover film, before not surviving after grafting, forbid to spray water, treat that the mycelia on scion and grafting point twists joint firmly, scion growing point recovers growth, after glossy ganoderma survives by normal management.
Described other kind glossy ganoderma of step (2) is selected from red sesame, purple sesame.
The present invention has also protected a kind of glossy ganoderma dish garden technique: the glossy ganoderma of cultivating in bag different cultivars; the ganoderma lucidum fruitbody that obtains different shape, different colours and differ in size; and according to the different heteroplastic graft methods of above-mentioned glossy ganoderma; the ganoderma lucidum fruitbody of selecting different shape, different colours and differ in size is as scion; grafting on different grafting points; the growth that is combined into one of the lucidum variety of different characteristics, produce glossy ganoderma dish garden.
Glossy ganoderma grafting method is generally to carry out between same kind or same bacterial strain, in the grafting scion of ganoderma lucidum fruitbody growing point place, away from growing point grafting scion, is being difficult for surviving.Adopt the bacterium ball of Ganoderma lucidum to be attached to grafting contact place, during grafting, after fixing scion, under adapt circumstance condition, bacterium ball is permanently fixed contact and scion after sprouting fast.The grafting point of glossy ganoderma is not subject to the restriction of growing point like this, and the scion of glossy ganoderma is not subject to the kind of contact or the restriction of bacterial strain.Be conducive to produce the potted landscape of making a lot of variety, application glossy ganoderma live body potted landscape technique has very strong advantage.
Strong according to ganoderma lucidum fruitbody regeneration capacity, aging after, quality is hard, has the natural gloss of paint sample, prolonged characteristic of being stale-proof, what artificial cultivating in bag was the most characteristic is the lucidum varieties such as red sesame, purple sesame, deer horn sesame.At ganoderma lucidum fruitbody vegetative stage, adopt artificial grafting method the growth that is combined into one of the lucidum variety of different characteristics, produce the colourful glossy ganoderma dish garden of polychrome, glossy ganoderma dish garden is simple and unsophisticated pure and fresh, scene is attractive in appearance, outstanding lucky implied meaning, enriched people's culture life, economic benefit and social benefit tool that further raising glossy ganoderma is produced are of great significance.
Embodiment
Embodiment mono-
1, the glossy ganoderma of conventional cultivating in bag different cultivars (red sesame, purple sesame, Ganoderma lucidum)
(1) batching pack.Culture medium prescription is: rice sweet oak wood chip 70%, wheat bran 20%, corn flour 7%, phosphate fertilizer 1%, gypsum 1% and brown sugar 1%.By formula, claim sufficient raw material, when preparation planting material, first the material such as wood chip, wheat bran, land plaster are mixed thoroughly, after sugar is water-soluble, mixes in material and mix thoroughly, the water content of material rests in 63%, pH value 5.8.Adopt the polypropylene plastics sacked material of 0.0045 centimetre of 17 cm x 33 cm x, limit rim compacting, degree of tightness is suitable, approximately 700 grams of every bag of weight in wet bases.Loud-speaker choma and sponge gag for sack.
(2) sterilizing of bacterium bag, inoculation.Sterilizing immediately after composts or fertilisers of cultivating pack.121 ℃ keep 2 hours.After sterilizing, take out inoculation when material temperature is cooled to below 30 ℃.Inoculation is undertaken by sterile working requirement, makes every effort to be swift in motion, accurately, and one bottle of (750ml bottle) wheat original seed, the 40 bags of left and right of bag that can connect material.Labelled after inoculation, indicate lucidum variety.
(3) bacterium bag is cultivated.
Vaccinated bacterium bag is moved in culturing room, be emitted on culturing rack, send out the bacterium stage, culturing room keeps 28 ℃, and relative air humidity 60% is secretly cultivated, and every 5 days bacterium, checks and prevent and treat living contaminants.When mycelium sends out full 3/4, move into mushroom producing room fruiting.
(4) bacterium bag management of producing mushroom
Bacterium sack in mushroom producing room is removed to sponge gag, intensity of illumination 200～300lx in control room, 25～26 ℃ of temperature, relative air humidity is brought up to 85-95%, controls suitable CO early stage
2concentration, promotes stem to extend.When stem reaches certain length, add intense light irradiation, sufficient oxygen is provided, promote cap to extend.Intensity of illumination 400～500lx in control room, ventilation every day secondary.Stem apical growing point expands, and forms gradually cap, when cap grows 2-3 circle, strengthen humidity, carries out water spray and ventilation operation.Red sesame cap is changed by white → pale yellow → Huang → bronzing, and purple sesame cap is changed by white → grey violet → puce.When cap edge, white disappears, and edge reddens or purpling, when the back side emits conidial powder, shows fruit body maturation, can gather.
2, conventional Ganoderma lucidum cell culture processes
(1) Ganoderma lucidum cell growth medium formula: potato 10%g, wheat bran 2.5%, corn flour 1.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, dusty yeast 0.5%, brown sugar 1%, pH6.
(2) sterilizing routinely, inoculation, shaking table shaken cultivation.
(3) obtain after filtration glossy ganoderma living cells (bacterium ball), for glossy ganoderma grafting point, connect and use.
3, the different heteroplastic graft methods of glossy ganoderma
Under the environmental condition of growing at suitable ganoderma lucidum fruitbody, adopt appropriate bacterium ball to put in the middle of scion and contact, fixing scion and contact, get final product grafting survival about a week.
(1) grafting environment: require surrounding air relative moisture to reach 85%～90%, 26～28 ℃ of temperature.Illumination 50 lx.
(2) scion: according to glossy ganoderma dish garden modeling requirement, select different shape, different colours and the ganoderma lucidum fruitbody that differs in size as scion, and scale off by sterile blade planar, put plastic sack interior stand-by.
(3) graft technology: on the grafting point away from Antler Mythic Fungus sporophore growing point, first cut 1 cutter with sharp blade by flat epidermis, bacterium ball and scion are put on contact, with the fixing scion of bamboo let and contact, and it is stuffy to cover film.Before not surviving after grafting, forbid water spray, by the management in a week, the mycelia on scion and contact will be twisted joint firmly, and scion growing point recovery is grown, and glossy ganoderma just can survive substantially, after surviving by normal management.
4, glossy ganoderma dish garden technique
According to the biological property of glossy ganoderma, by Artificial Control, cultivate the conditions such as light, temperature, water, gas in basket, reach fruit body and press down effect long, that encourage and change the direction of growth.Specific practice is:
(1) formation of control stem and cap, the bacterium bag that just forms fruit body primordium.Be placed on temperature, humidity and illumination all in suitable large plastic crate, plastic crate external application plastic foil encases, acanthopore on basket face, make a basket interior gas concentration lwevel reach 0.1%, at this moment the fruit body primordium in basket only extends stem and does not form cap, when stem grows to after certain length, bacterium bag is taken out and is placed on basket in basket, because ventilation is good, the stem extending can form cap very soon.
(2) quantity of control stem, bacterium bag is selected the small-bore collar, can form single creature sesame of long stem, and bacterium bag is selected the heavy caliber collar, grows the glossy ganoderma that grows thickly of many stems; Nutrient abundance, can grow the large-scale glossy ganoderma that a large handle is thick.
(3) change the direction that stem and cap are grown, according to glossy ganoderma, have strong phototropism, in the sporophore growth stage, adopt four sides shading, can make stem and cap grow vertically upward.If adopt the one-sided light of seeing, can make stem and cap towards there being direction of light growth.
(4) cultivation of double-deck cap, forming cap and not dormant glossy ganoderma, then puts into plastic crate and continues to cultivate, under poor aeration environmental condition, below cap, just there will be hyperplasia layer, cap is thickened, hyperplasia layer edge can grow secondary stem and continue and extend.When stem reach a certain height, take out bacterium bag, recover good aeration condition, secondary stem grows cap very soon, forms the glossy ganoderma of double-deck cap.
(5) fruit body that forms distortion uses ultraviolet ray to irradiate, and can make to be out of shape in entity.With plastic paper wrapping, can make stem bending, the chap of growing, or occur the cap wilfully scabbing.
(6) the fruit-body color depth, is subject to the impact of light power, and the strong fruit-body color of light is dark, otherwise shallow.
(7) glossy ganoderma dish garden
Can produce following potted landscape:
1. a pair of mandarin duck: take a pair of Ganoderma lucidum as mobile jib, be each one of the red sesame of 3～5 centimetres, purple sesame at 8～10 cm height grafting bacteria cover diameters.
2. three state close friends, cultivate three Ganoderma lucidums, and wherein two root graft bacteria cover diameters are each one of 3～5 centimetres of red sesames, purple sesame.
3. a skill is solely elegant: cultivate many Ganoderma lucidums, with hormone control growth with promote growth, make one long sturdy obvious.
4. fresh breeze promotion: three layers of red sesame or purple sesame graftings, one deck is than a floor height.
5. set each other off with one heart: cultivate two Ganoderma lucidums, grafting diameter is each one of the red sesame of 3～5 centimetres, purple sesame, then is integrated.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (1)
1. the different heteroplastic graft methods of glossy ganoderma, is characterized in that: said method comprising the steps of:
(1) cultivating in bag Ganoderma lucidum, obtains deer horn shape fruit body, and obtains bacterium ball by Ganoderma lucidum liquid culture;
(2) other kind glossy ganoderma of cultivating in bag, obtains mature sporophore, and its cap is kidney shape, semicircle or subcircular, using this fruit body as scion, and along base portion truncation, gets off with sterile blade;
(3) the different heteroplastic graft methods of glossy ganoderma:
A, grafting environment: require surrounding air relative moisture to reach 85%～90%, 26～28 ℃ of temperature, illumination every day 12 hours, intensity of illumination 50 lx;
B, grafting: take deer horn shape sporophore growth point or away from growing point place as grafting point, on grafting point, first with sharp blade, epidermis is put down and cut 1 cutter, bacterium ball and scion are put to grafting point above, and with the fixing scion of bamboo let and grafting point, and it is stuffy to cover film, before not surviving after grafting, forbid to spray water, treat that the mycelia on scion and grafting point twists joint firmly, scion growing point recovers growth, after glossy ganoderma survives by normal management;
Described other kind glossy ganoderma of step (2) is selected from red sesame, purple sesame;
The glossy ganoderma of cultivating in bag different cultivars, the ganoderma lucidum fruitbody that obtains different shape, different colours and differ in size, and according to the different heteroplastic graft methods of glossy ganoderma, the ganoderma lucidum fruitbody of selecting different shape, different colours and differ in size is as scion, grafting on different grafting points, the growth that is combined into one of the lucidum variety of different characteristics, produce glossy ganoderma dish garden.
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| CN104541938B (en) * | 2013-10-18 | 2018-06-08 | 杭州常青保健食品有限公司 | A kind of ganoderma lucidum merges cultural method |
| CN103688750B (en) * | 2013-12-16 | 2015-09-09 | 福建农林大学 | A kind of preparation method of live body glossy ganoderma dish garden |
| CN106818205A (en) * | 2016-12-19 | 2017-06-13 | 蚌埠鲲鹏食品饮料有限公司 | A kind of red sesame root process |
| CN108293594A (en) * | 2017-10-20 | 2018-07-20 | 翔天农业开发集团股份有限公司 | A kind of moulding cultural method of ganoderma lucidum landscape type |
| CN108587924B (en) * | 2018-04-20 | 2021-02-12 | 湖南农业大学 | Ganoderma lucidum mycelium pellet product |
| CN111512883A (en) * | 2019-02-02 | 2020-08-11 | 李志广 | Method for planting ornamental ganoderma lucidum antler and paint |
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| CN101317537A (en) * | 2008-07-18 | 2008-12-10 | 武传锋 | Transplantation and graft method for glossy ganoderma |
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| JPH1042694A (en) * | 1996-07-29 | 1998-02-17 | Yukio Takai | Infection of mycorrhizal fungus by grafting method |
| KR101006734B1 (en) * | 2008-10-14 | 2011-01-10 | 차준돈 | Method of culturing ganoderma lucidum karst |
| KR101165269B1 (en) * | 2009-12-22 | 2012-07-19 | 박정헌 | Method for plant in a pot of forest cultivating using Ganoderma lucidum |
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| JP特开平10-42694A 1998.02.17 |
| 宫志远等.1.《灵芝 蛹虫草 天麻栽培与加工新技术》.中国农业出版社,2005,第19页. * |
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