CN105379557A - Method for conducting living body graft on lucid ganoderma bonsai through cut log - Google Patents

Method for conducting living body graft on lucid ganoderma bonsai through cut log Download PDF

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Publication number
CN105379557A
CN105379557A CN201510755697.8A CN201510755697A CN105379557A CN 105379557 A CN105379557 A CN 105379557A CN 201510755697 A CN201510755697 A CN 201510755697A CN 105379557 A CN105379557 A CN 105379557A
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water
lucid ganoderma
fruit body
medium
glossy ganoderma
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孔云
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Wuxi County Yunxiang Edible Shares Professional Cooperatives
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Wuxi County Yunxiang Edible Shares Professional Cooperatives
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mycology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to a method for conducting living body grafting on lucid ganoderma bonsai through a cut log. The method includes the specific steps that 1, a fresh tree with the diameter ranging from 15 cm to 21 cm is selected, scraped off and cut into segments with the length ranging from 18 cm to 21 cm, punching is conducted, and hole sealing is conducted through bark; 2, products obtained in step 1 are bagged and sterilized, and strains are grafted to the cut log for cultivation; 3, a lucid ganoderma cultivation medium with bacteria fermented ready is placed into a flowerpot to be cultivated till sporocarp is mature by 70%; 4, grafting is conducted, wherein the sporocarp on the cup log in a vaccination room serves as an ear and inserted into the sporocarp in the flowerpot, and the circulation condition is controlled; 5, bonsai management is conducted, after lucid ganoderma grows well, modification is conducted, and the lucid ganoderma is placed into a ceramic pot. By means of the method, the technical problem that an existing lucid ganoderma bonsai is lifeless and not vital is solved. According to the lucid ganoderma bonsai manufactured through the method, the quality of the lucid ganoderma bonsai is remarkably improved.

Description

Duan Mu is utilized to carry out the method for live body grafting glossy ganoderma dish garden
Technical field
The present invention relates to a kind of making method for its potted landscape, be specially a kind of method that Duan Mu of utilization carries out live body grafting glossy ganoderma dish garden.
Background technology
Glossy ganoderma, also known as Lin Zhongling, celestial grass, refreshing grass, auspicious grass, is Basidiomycetes Polyporaceae Ganoderma fungi.Glossy ganoderma have strengthen the body resistance to consolidate the constitution, invigorating qi for tranquilization, relieving cough and asthma effect, be the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, there is very high medical value.
Existing glossy ganoderma dish garden is mostly that the dry product glossy ganoderma crossed through artificial treatment on streamline is put together, the ornaments of dry product both not angry, there is no vitality, more without health value and edible medical value.
Summary of the invention
In view of this, a kind of Duan Mu of utilization is the object of the present invention is to provide to carry out the method for live body grafting glossy ganoderma dish garden.
For achieving the above object, the technical solution used in the present invention is as follows:
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh trees of 15-21cm, and rossing, is cut into the section that length is 18-21cm, punching, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and to cultivate in strain inoculation to Duan Mu;
C. the Medium for Ganoderma lucidum sending out bacterium good is placed in flowerpot and is cultured to fruit body medium well;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Glossy ganoderma, also known as Lin Zhongling, celestial grass, refreshing grass, auspicious grass, is Basidiomycetes Polyporaceae Ganoderma fungi.Glossy ganoderma have strengthen the body resistance to consolidate the constitution, invigorating qi for tranquilization, relieving cough and asthma effect, be the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, there is very high medical value.
Glossy ganoderma can launch out conidial powder growing to the stage of ripeness, and the medical value of conidial powder, than glossy ganoderma height decades of times, is the elite of glossy ganoderma.Conidial powder can purify the air of a room, after people absorbs, can tranquilizing and allaying excitement, and very useful to health; Can film cover bag collection enjoy when the ripe conidial powder of new sesame is flown away in a large number.Therefore have colourful fresh and alive glossy ganoderma dish garden, be not only room or office space and make something perfect even more perfect, but also can play and allow people's spirit and QI cleaning, contribute to one's peace of mind of inner tranquility, develop immunitypty, regulates effect of health each side function.
In steps A, Duan Mu is cut into the section that length is 18-21cm, is applicable to the production of glossy ganoderma, and the pack of composts or fertilisers of cultivating can not be hindered.Being punched by section wood is then be convenient to ganoderma lucidum mycelium sprout on Duan Mu.
Further, trees described in steps A are robur, evergreen chinquapin or beech tree.Utilize the branch section of this several tree to cultivate glossy ganoderma, glossy ganoderma fast growth, output be high, color and luster good, real in fruit body.
Further, the degree of depth in hole described in steps A is 3-3.5cm, and diameter is 1.3-1.5cm, and angle is 50-65 °.The sprouting of ganoderma lucidum mycelium on Duan Mu, growth is conducive under this condition.
Sterilizing described in step B be by charging after pocket in 100 DEG C of sterilizing 8-12 hour, or in 120 DEG C of sterilizing 1-2 hour.
Further, the temperature of cultivating described in step B and step C is 20-30 DEG C, and relative moisture is 80-85%.Under this condition, be conducive to the growth of glossy ganoderma.
Further, in incubation described in step C, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g.Triacontanol is a kind of natural Long carbon chain plant growth regulator, chemical name N-triacontanol, structural formula CH 3(CH 2) 28cH 2oH, also known as melissyl alcohol, is the natural biological product of purification from honeybee wax, to person poultry harmless and side effect, and environmentally safe.Use it for the growth regulating of glossy ganoderma, the incubation time of lucidum strain can be shortened, enhance productivity.
Further, the preparation method of medium described in step C is: cotton seed hulls 25-30 part, stalk section 15-25 part, wheat bran 6-15 part, corncob section 15-25 part and land plaster 3 parts are mixed, add after water-soluble for 2 parts, brown sugar 20-30 part is formed brown sugar water, then be 60-70% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described part is mass parts.Medium prepared by the method, its component and proportioning are suitable for, under the synergy of each component, for Ganoderma lucidum mycelium bulk-growth provides sufficient carbon source, nitrogenous source, trace element, supporter etc., balanced in nutrition, are applicable to sprouting and the growth of ganoderma lucidum mycelium.
Further, described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation.This operation is conducive to moisture and fully enters medium inside, and moisture is not easy to scatter and disappear, and improves medium moisture capacity.
Further, the pH of described water is 5.5-6.Under this pH environment, glossy ganoderma well-grown.
The invention has the beneficial effects as follows:
The glossy ganoderma dish garden adopting the method for the invention to prepare, significantly improves the quality of glossy ganoderma dish garden.Compared with prior art, the complete Reishi sporule number contained in every gram of conidial powder of the glossy ganoderma dish garden adopting the method for the invention to prepare improves 30.47%; The sporoderm-broken rate of ganoderma spove powder improves 29.73%.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearly, below embodiments of the invention are described in detail.
Embodiment 1
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh robur of 15cm, and rossing, is cut into the section that length is 21cm, punching, and the degree of depth in hole is 3cm, and diameter is 1.5cm, and angle is 65 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 30 DEG C in temperature, relative moisture be cultivate under 80% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 8 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 20 DEG C, and relative moisture is be cultured to fruit body medium well under 85% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 30kg, stalk section 15kg, wheat bran 15kg, corncob section 15kg and land plaster 3kg are mixed, add after water-soluble for brown sugar 2kg 20kg is formed brown sugar water, then be 70% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.5;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Embodiment 2
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh beech tree of 21cm, and rossing, is cut into the section that length is 18cm, punching, and the degree of depth in hole is 3.5cm, and diameter is 1.3cm, and angle is 50 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 20-30 DEG C in temperature, relative moisture be cultivate under 80-85% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 12 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 30 DEG C, and relative moisture is be cultured to fruit body medium well under 80% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 25kg, stalk section 25kg, wheat bran 6kg, corncob section 25kg and land plaster 3kg are mixed, and adds after water-soluble for brown sugar 2kg 30kg is formed brown sugar water, then is 60% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 6;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Embodiment 3
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh evergreen chinquapin of 18cm, and rossing, is cut into the section that length is 20cm, punching, and the degree of depth in hole is 3.3cm, and diameter is 1.4cm, and angle is 60 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 25 DEG C in temperature, relative moisture be cultivate under 83% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 10 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 25 DEG C, and relative moisture is be cultured to fruit body medium well under 82% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 28kg, stalk section 20kg, wheat bran 9kg, corncob section 20kg and land plaster 3kg are mixed, and adds after water-soluble for brown sugar 2kg 25kg is formed brown sugar water, then is 65% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.8;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Embodiment 4
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh robur of 20cm, and rossing, is cut into the section that length is 19cm, punching, and the degree of depth in hole is 3.4cm, and diameter is 1.3cm, and angle is 55 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 24 DEG C in temperature, relative moisture be cultivate under 81% condition; Described sterilizing be by charging after pocket in 120 DEG C of sterilizings 1 hour;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 24 DEG C, and relative moisture is be cultured to fruit body medium well under 84% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 23kg, stalk section 18kg, wheat bran 10kg, corncob section 19kg and land plaster 3kg are mixed, add after water-soluble for brown sugar 2kg 26kg is formed brown sugar water, then be 64% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.6;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin
Embodiment 5
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh beech tree of 16cm, and rossing, is cut into the section that length is 19cm, punching, and the degree of depth in hole is 3.2cm, and diameter is 1.3cm, and angle is 62 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 28 DEG C in temperature, relative moisture be cultivate under 81% condition; Described sterilizing be by charging after pocket in 120 DEG C of sterilizings 2 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 27 DEG C, and relative moisture is be cultured to fruit body medium well under 82% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 26kg, stalk section 21kg, wheat bran 12kg, corncob section 23kg and land plaster 3kg are mixed, add after water-soluble for brown sugar 2kg 26kg is formed brown sugar water, then be 67% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.7;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin
Reference examples 1
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh maple of 20cm, and rossing, is cut into the section that length is 20cm, punching, and the degree of depth in hole is 2.8cm, and diameter is 1.6cm, and angle is 48 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 26 DEG C in temperature, relative moisture be cultivate under 82% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 10 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 23 DEG C, and relative moisture is be cultured to fruit body medium well under 82% condition; The preparation method of medium is: cotton seed hulls 26kg, stalk section 20kg, wheat bran 9kg and land plaster 3kg are mixed, and adds after water-soluble for brown sugar 2kg 27kg is formed brown sugar water, then is 61% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.5-6;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Reference examples 2
Utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. selection diameter is the fresh beech tree of 20cm, and rossing, is cut into the section that length is 19cm, punching, and the degree of depth in hole is 3.8cm, and diameter is 1.6cm, and angle is 75 °, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and will strain inoculation to Duan Mu be 23 DEG C in temperature, relative moisture be cultivate under 84% condition; Described sterilizing be by charging after pocket in 120 DEG C of sterilizings 1.5 hours;
C. the Medium for Ganoderma lucidum sending out bacterium good being placed in flowerpot in temperature is 25 DEG C, and relative moisture is be cultured to fruit body medium well under 84% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g; The preparation method of medium is: cotton seed hulls 26kg, stalk section 18kg, wheat bran 11kg, corncob section 21kg and land plaster 3kg are mixed, add after water-soluble for brown sugar 2kg 23kg is formed brown sugar water, then be 66% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation; The pH of described water is 5.6;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
Reference examples 3
With publication number for method described in CN103026897A makes live body glossy ganoderma dish garden.Its concrete steps are:
A. cutting down diameter is the trees of 21cm, in the segment on the same day of slaking sterilization, length is 30cm, loads in plastic sack by section wood, in transfer room, sterilizing is carried out 12 hours to section wood, according to the inoculum concentration of 1 cubic metre of section wood 100 bag bacterial classification, lucidum strain is inoculated into incubation growth on described section of wood;
B. the Medium for Ganoderma lucidum sending out bacterium good is placed on incubation growth in the flowerpot in plastic greenhouse, when fruit body grows to medium well, fruit body on the wood of transfer room stage casing is inserted in the fruit body in flowerpot as skewer fringe, control the ventilation condition having formed cap and not dormant glossy ganoderma, formed and thicken cap and pileolus, make it force growth according to certain moulding wrapping the glossy ganoderma grown, after ganoderma lucidum fruitbody is formed, controlled light condition changes the color of glossy ganoderma;
C. when modeled lucid ganoderma is grown well, waxing, modification, load in pottery basin, and add filler.
Be provided with temperature control equipment in described transfer room, humidity control apparatus and illumination regulation device control the growing environment of lucidum strain.
The tangent plane of the Duan Mu cut down in described steps A is smooth, and around corner angle scabble.
Use the tally of 25 middle lattice × 16 little lattice, calculate the sporoderm-broken rate of complete Reishi sporule number and the ganoderma spove powder contained in every gram of conidial powder of the glossy ganoderma dish garden (being respectively experimental group 1,2,3,4,5) of embodiment 1-5 making and the glossy ganoderma dish garden (being respectively control group 1,2,3) of reference examples 1-3 making.Result is as shown in the table:
Remarks: the sporoderm-broken rate of the complete Reishi sporule number contained in every gram of conidial powder, ganoderma spove powder measures according to People's Republic of China's professional standard " GB/T29344-2012 lucidum spore powder is gathered and process technology specification ".
As seen from the above table, experimental group make glossy ganoderma dish garden every gram of conidial powder in contain complete Reishi sporule number, ganoderma spove powder sporoderm-broken rate all higher than control group.Compared with control group 3, the complete Reishi sporule number contained in every gram of conidial powder of the glossy ganoderma dish garden that experimental group makes improves 30.47%; The sporoderm-broken rate of ganoderma spove powder improves 29.73%.Prove thus, adopt the method for the invention to carry out glossy ganoderma dish garden making, significantly improve the quality of glossy ganoderma dish garden.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, those of ordinary skill in the art is to be understood that, can modify to technical scheme or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.

Claims (9)

1. utilize Duan Mu to carry out the method for live body grafting glossy ganoderma dish garden, it is characterized in that, concrete steps are:
A. selection diameter is the fresh trees of 15-21cm, and rossing, is cut into the section that length is 18-21cm, punching, and seals with bark;
B. steps A gained is carried out packing, sterilizing, and to cultivate in strain inoculation to Duan Mu;
C. the Medium for Ganoderma lucidum sending out bacterium good is placed in flowerpot and is cultured to fruit body medium well;
D. grafting: the fruit body on the wood of transfer room stage casing be inserted in the fruit body in flowerpot as skewer fringe, controls ventilation condition;
E. potted landscape management: glossy ganoderma grows well, modifies, loads in pottery basin.
2. method according to claim 1, is characterized in that, trees described in steps A are robur, evergreen chinquapin or beech tree.
3. method according to claim 1, is characterized in that, the degree of depth in hole described in steps A is 3-3.5cm, and diameter is 1.3-1.5cm, and angle is 50-65 °.
4. method according to claim 1, is characterized in that: sterilizing described in step B be by charging after pocket in 100 DEG C of sterilizing 8-12 hour, or in 120 DEG C of sterilizing 1-2 hour.
5. preparation method according to claim 1, is characterized in that: the temperature of cultivating described in step B and step C is 20-30 DEG C, and relative moisture is 80-85%.
6. method according to claim 1, is characterized in that: in incubation described in step C, and fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g.
7. method according to claim 1, it is characterized in that, the preparation method of medium described in step C is: cotton seed hulls 25-30 part, stalk section 15-25 part, wheat bran 6-15 part, corncob section 15-25 part and land plaster 3 parts are mixed, add after water-soluble for 2 parts, brown sugar 20-30 part is formed brown sugar water, then be 60-70% with water sprinkling composts or fertilisers of cultivating to its total moisture content; Described part is mass parts.
8. method according to claim 7, is characterized in that: described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation.
9. the method according to claim 7 or 8, is characterized in that: the pH of described water is 5.5-6.
CN201510755697.8A 2015-11-09 2015-11-09 Method for conducting living body graft on lucid ganoderma bonsai through cut log Pending CN105379557A (en)

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN106818205A (en) * 2016-12-19 2017-06-13 蚌埠鲲鹏食品饮料有限公司 A kind of red sesame root process
CN115176641A (en) * 2021-04-01 2022-10-14 河源市神农宝灵芝生产经营专业合作社 Method for cultivating black flower lucid ganoderma by utilizing illumination

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CN103026897A (en) * 2012-12-11 2013-04-10 昭平县农业局 Lucid ganoderma bonsai grafting method

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