CN115176641B - Method for cultivating ganoderma atrum by utilizing illumination - Google Patents
Method for cultivating ganoderma atrum by utilizing illumination Download PDFInfo
- Publication number
- CN115176641B CN115176641B CN202110365108.0A CN202110365108A CN115176641B CN 115176641 B CN115176641 B CN 115176641B CN 202110365108 A CN202110365108 A CN 202110365108A CN 115176641 B CN115176641 B CN 115176641B
- Authority
- CN
- China
- Prior art keywords
- ganoderma
- ganoderma lucidum
- atrum
- cultivation
- basswood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for cultivating ganoderma atrum by utilizing illumination, which belongs to the technical field of ganoderma lucidum cultivation, and the cultivated ganoderma atrum comprises the following steps: the invention adopts an induction type process to mold, changes natural conditions such as illumination intensity, light direction, temperature and humidity and the like affecting the growth of ganoderma lucidum in different growth periods of ganoderma lucidum through artificial regulation and control, so that the ganoderma lucidum presents an artistic mold with beautiful flower shape, the whole process is natural culture, the process is simple and easy to operate, and the cultivated ganoderma atrum has beautiful appearance, strong ornamental value and prominent spirit and auspicious meaning of ganoderma lucidum like peacock spreading; the cultivation method of the ganoderma atrum provided by the invention is different from the traditional technology, no chemical component is added in the cultivation process, the ganoderma atrum is distinct in level, and the cultivation method can be applied to the decoration of high-grade rooms, offices and hotels, and the ornamental value and economic benefit of the ganoderma atrum are greatly improved.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum cultivation, in particular to a method for cultivating ganoderma atrum by utilizing illumination.
Background
The ganoderma lucidum has extremely high position in the edible and medicinal fungi and has wide medicinal efficacy, and is used as a auspicious place for ancient times due to attractive appearance and rare and difficult obtainment. In recent years, the economic value of ganoderma lucidum artwork is higher and higher, more and more ganoderma lucidum manufacturers pay more attention to the manufacturing and production of ganoderma lucidum bonsai, but most of ganoderma lucidum bonsai manufacturing technologies adopt traditional manufacturing technologies.
In the prior art, the ganoderma cultivated by the conventional method has short growth period of living ganoderma, is not easy to store, has thin ganoderma lucidum stems, few branches and poor hierarchy, and has no ornamental value; the traditional glossy ganoderma bonsai manufacturing technology generally adopts grafting, bonding, painting and other methods to change the shape of the glossy ganoderma into various shapes, has luxurious luster, but has the advantages of short storage time, low safety and limited artistic creativity.
Accordingly, the prior art has drawbacks and needs improvement.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: the method for cultivating the ganoderma atrum by utilizing the illumination has the advantages of natural cultivation, no painting in the process of finished product preparation, no addition of any chemical components, rich layers and good ornamental value.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a method for cultivating Ganoderma atrum by using illumination comprises: the method comprises the following steps:
1) Preparation of a culture medium: the culture medium comprises the following raw materials in percentage by mass: wood chips 35%, wheat bran 45%, corn flour 15%, quartz 3% and brown sugar 2%, and adding water into the raw materials of the culture medium for uniform mixing to obtain the culture medium;
2) Basswood manufacturing: selecting lychee wood as a cultivation material, cutting the lychee wood into basswood with the length of 20-30 cm, drying under natural conditions until the water content of the lychee basswood is reduced to 30-35%, sleeving the lychee basswood and a culture medium into a cultivation bag, and then sterilizing;
3) Inoculating: after sterilization, inoculating ganoderma atrum strain into the cultivation bag in a sterile environment;
4) Hypha culture: transferring the cultivation bag into a cultivation room for cultivation after inoculation, keeping darkness in the cultivation room, controlling the temperature at 23-26 ℃ and the air humidity at 55-65%, and ventilating 3-4 times per day for 20-30 min each time until mycelia are fully distributed on the surface layer of the lychee basswood;
5) Burying soil and producing ganoderma lucidum: placing the lychee basswood full of mycelia in furrows of a greenhouse in a bag-removing manner, covering soil on the lychee basswood, and culturing for 30-40 days to obtain fruiting bodies, wherein the temperature of the greenhouse is kept between 24-27 ℃ and the air humidity is kept between 70-90%, and the pH value of soil is controlled between 5 and 6;
6) And (3) modeling control:
a: the mycelium is in a bud stage after being differentiated, at the moment, the greenhouse environment is cooled to 16-20 ℃, a light source is adopted to irradiate the fruiting body, the light source is vertically arranged right above the fruiting body during irradiation, the illumination intensity is controlled to be 400-900 Lux, and the cultivation is continued for 13-16 days, so that the ganoderma atrum with the flower bud in a full-open shape is directionally cultivated;
b: increasing the illumination intensity to 1800-3600 Lux, controlling the air humidity to be 80-95%, introducing carbon dioxide into the greenhouse to control the carbon dioxide concentration in the greenhouse to be 0.1-0.3%, lasting for 10-15 days, cutting a V-shaped groove on a blade or a ganoderma lucidum handle of a fruiting body when the ganoderma lucidum handle is 6-13 cm long, cutting the bottom of a ganoderma lucidum fungus foot to be grafted into a sharp block matched with the V-shaped groove, fixing the sharp block of the ganoderma lucidum fungus foot into the V-shaped groove of the grafted fruiting body by using a bracket, and dismantling the bracket after healing of a grafted wound;
c: after the ganoderma lucidum is grafted to survive or the ganoderma atrum main body is basically formed, the control temperature in the greenhouse is kept at 23-26 ℃, the air humidity is kept at 80-90%, the illumination intensity is controlled at 1500-2500 Lux, and the pH value of soil is controlled at pH6;
d: after the black flower ganoderma lucidum grows and matures, the cultivation bag sleeved outside the lychee basswood can be removed, the surface of the lychee basswood is kept dry, and the shaped black flower ganoderma lucidum is subjected to local trimming and then is harvested.
Preferably, the pH value of the culture medium is 6, and the water content of the culture medium is 60-65%.
Preferably, the cultivation bag is made of polyethylene.
Preferably, the light source is an LED light source, the distance between the LED light source and the fruiting body is 40-70 cm, and the LED light source is turned off in a night state.
Preferably, the sterilization treatment is an atmospheric sterilization or autoclaving treatment method;
the normal pressure sterilization treatment method comprises the following steps: placing the cultivation bag in a sterilizing cabinet, wherein the temperature in the sterilizing cabinet is 100 ℃, and the sterilizing time is 4-5 hours;
the high-pressure sterilization treatment method comprises the following steps: placing the cultivation bag in an autoclave, and continuously heating in the autoclave until the pressure in the autoclave rises to 0.11-0.13 MPa, and stopping heating after keeping for 1.5-3 h.
Preferably, after the ganoderma lucidum is grafted and survived, the ganoderma lucidum with the edge of the ganoderma lucidum cover being differentiated is arranged in the opposite direction, and illumination with the illumination intensity of 1500-2000 Lux is given to the back surface of the ganoderma lucidum cover for 3-4 days until new primordia are differentiated from the back surface of the illuminated ganoderma lucidum cover, and the primordia can be continuously differentiated along the ganoderma lucidum cover, so that the ganoderma lucidum with the black flower of the multi-face ganoderma lucidum cover is formed.
Compared with the prior art, the invention has the following beneficial effects:
1. the cultivation method of the ganoderma atrum disclosed by the invention is different from the traditional technology, the ganoderma atrum is molded by adopting an induction type process, natural conditions such as illumination intensity, light direction, temperature and humidity and the like which influence the growth of the ganoderma lucidum are changed in different growth periods of the ganoderma lucidum through artificial regulation, so that the ganoderma lucidum presents an artistic model with beautiful flower shape in the growth process, the whole process is natural culture, the finished product manufacturing process is free of painting and adding any chemical component, no toxic component is added, and the product can be stored for many years and has the same color as the first year;
2. the cultivation method of the ganoderma atrum disclosed by the invention is simple in process and easy to operate, and the survival rate of the ganoderma atrum can be effectively improved by adding concentrated nutrition supply, so that the cultivated ganoderma atrum fruiting body is rapid in growth and strong in flower, and has high medicinal value;
3. the ganoderma atrum cultivated by the invention has beautiful appearance and strong ornamental value, and highlights the spirit and lucky meaning of ganoderma atrum, can be applied to the decoration of high-grade rooms, offices and hotels, and greatly improves the ornamental value and economic benefit of ganoderma atrum.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
The embodiment provides a method for cultivating ganoderma atrum by utilizing illumination, which comprises the following steps: the method comprises the following steps:
1) Preparation of a culture medium: the culture medium comprises the following raw materials in percentage by mass: 35% of wood chips, 45% of wheat bran, 15% of corn meal, 3% of quartz and 2% of brown sugar, and adding water into the raw materials of the culture medium to uniformly mix to obtain the culture medium.
Further, the pH value of the culture medium is 6, and the water content of the culture medium is 60-65%. In this example, the water content of the medium is preferably 60%.
2) Basswood manufacturing: the litchi wood is selected as a cultivation material, cut into basswood of 20-30 cm, dried under natural conditions until the water content of the litchi basswood is reduced to 30-35%, and the litchi basswood and a culture medium are sleeved with a cultivation bag and then subjected to sterilization treatment. In this example, the length of litchi wood is preferably 20cm, and drying is performed under natural conditions until the water content of the litchi basswood is reduced to 30%.
Further, the cultivation bag is made of polyethylene.
Further, the sterilization treatment is a normal pressure sterilization or high pressure sterilization treatment method;
the normal pressure sterilization treatment method comprises the following steps: placing the cultivation bag in a sterilizing cabinet, wherein the temperature in the sterilizing cabinet is 100 ℃, and the sterilizing time is 4-5 hours;
the high-pressure sterilization treatment method comprises the following steps: placing the cultivation bag in an autoclave, and continuously heating in the autoclave until the pressure in the autoclave rises to 0.11-0.13 MPa, and stopping heating after keeping for 1.5-3 h.
In this embodiment, the sterilization treatment is preferably an atmospheric sterilization treatment method, specifically: placing the cultivation bag in a sterilizing cabinet, wherein the temperature in the sterilizing cabinet is 100 ℃, and the sterilizing time is 4 hours.
3) Inoculating: after sterilization, inoculating black ganoderma lucidum strain into the cultivation bag in a sterile environment.
4) Hypha culture: transferring the cultivation bag into a cultivation room for cultivation after inoculation, keeping darkness in the cultivation room, controlling the temperature at 23-26 ℃ and the air humidity at 55-65%, ventilating 3-4 times per day for 20-30 min each time until mycelia are fully distributed on the surface layer of the lychee basswood. In this example, the temperature is preferably controlled at 23℃and the air humidity is maintained at 55% and ventilation is carried out 3 times a day for 20 minutes each.
5) Burying soil and producing ganoderma lucidum: placing the lychee basswood bag which is fully covered with hyphae into a furrow of a greenhouse, covering soil on the lychee basswood, and culturing for 30-40 days to obtain fruiting bodies, wherein the temperature of the greenhouse is kept between 24-27 ℃ and the air humidity is kept between 70-90%, and the pH value of soil is controlled between 5 and 6 in the culturing process. In this example, it is preferable that the temperature of the greenhouse is kept at 24℃and the air humidity is kept at 70%, and the pH of the soil is controlled at pH5.
6) And (3) modeling control:
a: and (3) after the mycelium is differentiated, the mycelium is in a bud stage, at the moment, the greenhouse environment is cooled to 16-20 ℃, the fruiting body is irradiated by adopting a light source, and the light source is vertically arranged right above the fruiting body during irradiation, and the illumination intensity is controlled to be 400-900 Lux for 13-16 days so as to directionally culture the ganoderma atrum in a bud bloom shape. In this example, it is preferable that the temperature of the greenhouse environment is lowered to 16℃after the mycelium is differentiated, and the illumination intensity is controlled at 400Lux for 13 days during irradiation.
Further, the light source is an LED light source, the distance between the LED light source and the fruiting body is 40-70 cm, and the LED light source is turned off in a night state. In this embodiment, the distance between the LED light source and the fruiting body is preferably 40cm.
b: increasing the illumination intensity to 1800-3600 Lux, controlling the air humidity to be 80-95%, introducing carbon dioxide into the greenhouse, controlling the carbon dioxide concentration in the greenhouse to be 0.1-0.3%, lasting for 10-15 days, cutting a V-shaped groove on a blade or a ganoderma lucidum handle of a fruiting body when the length of the ganoderma lucidum handle is 6-13 cm, cutting the bottom of a ganoderma lucidum fungus foot to be grafted into a sharp block matched with the V-shaped groove, fixing the sharp block of the ganoderma lucidum fungus foot to the V-shaped groove of the grafted fruiting body by using a bracket, and dismantling the bracket after healing of a grafted wound. In this example, it is preferable to increase the illumination intensity to 1800Lux, control the air humidity to 80%, and introduce carbon dioxide into the greenhouse to control the carbon dioxide concentration in the greenhouse to 0.1%, for 10 days, when the length of the stem is as long as 6cm, a V-shaped groove is cut on the leaf or stem of the fruiting body, and the depth of the V-shaped groove is 2mm.
Further, after the ganoderma lucidum is grafted and survived, the ganoderma lucidum with the edge of the ganoderma lucidum cover being differentiated is arranged in the opposite direction, and illumination with the illumination intensity of 1500-2000 Lux is given to the back surface of the ganoderma lucidum cover for 3-4 days until new primordia are differentiated from the back surface of the illuminated ganoderma lucidum cover, and the primordia can be continuously differentiated along the ganoderma lucidum cover, so that the ganoderma atrum with multi-face ganoderma lucidum covers is formed. In this example, it is preferable to apply light with a light intensity of 1500Lux to the back surface of the cover for 3 days.
c: after the ganoderma lucidum is grafted to survive or the ganoderma atrum main body is basically formed, the control temperature in the greenhouse is kept at 23-26 ℃, the air humidity is kept at 80-90%, the illumination intensity is controlled at 1500-2500 Lux, and the pH value of soil is controlled at pH6. In this example, it is preferable to control the temperature in the greenhouse to 23℃and the air humidity to 80%, and to control the light intensity to 1500Lux.
d: after the black flower ganoderma lucidum grows and matures, the cultivation bag sleeved outside the lychee basswood can be removed, the surface of the lychee basswood is kept dry, and the shaped black flower ganoderma lucidum is subjected to local trimming and then is harvested.
Example 2
The embodiment provides a method for cultivating ganoderma atrum by utilizing illumination, which comprises the following steps: the method comprises the following steps:
1) Preparation of a culture medium: the culture medium comprises the following raw materials in percentage by mass: 35% of wood chips, 45% of wheat bran, 15% of corn meal, 3% of quartz and 2% of brown sugar, and adding water into the raw materials of the culture medium to uniformly mix to obtain the culture medium.
Further, the pH value of the culture medium is 6, and the water content of the culture medium is 60-65%. In this example, the water content of the medium is preferably 65%.
2) Basswood manufacturing: the litchi wood is selected as a cultivation material, cut into basswood of 20-30 cm, dried under natural conditions until the water content of the litchi basswood is reduced to 30-35%, and the litchi basswood and a culture medium are sleeved with a cultivation bag and then subjected to sterilization treatment. In this example, the length of litchi wood is preferably 30cm, and drying is performed under natural conditions until the moisture content of the litchi basswood is reduced to 35%.
Further, the cultivation bag is made of polyethylene.
Further, the sterilization treatment is a normal pressure sterilization or high pressure sterilization treatment method;
the normal pressure sterilization treatment method comprises the following steps: placing the cultivation bag in a sterilizing cabinet, wherein the temperature in the sterilizing cabinet is 100 ℃, and the sterilizing time is 4-5 hours;
the high-pressure sterilization treatment method comprises the following steps: placing the cultivation bag in an autoclave, and continuously heating in the autoclave until the pressure in the autoclave rises to 0.11-0.13 MPa, and stopping heating after keeping for 1.5-3 h.
In this embodiment, the sterilization treatment is preferably an autoclave sterilization treatment method, specifically: placing the cultivation bag in an autoclave, heating in the autoclave until the pressure in the autoclave rises to 0.11MPa, and stopping heating after maintaining for 1.5 h.
3) Inoculating: after sterilization, inoculating black ganoderma lucidum strain into the cultivation bag in a sterile environment.
4) Hypha culture: transferring the cultivation bag into a cultivation room for cultivation after inoculation, keeping darkness in the cultivation room, controlling the temperature at 23-26 ℃ and the air humidity at 55-65%, ventilating 3-4 times per day for 20-30 min each time until mycelia are fully distributed on the surface layer of the lychee basswood. In this example, the temperature is preferably controlled at 26℃and the air humidity is maintained at 65% and ventilation is carried out 4 times a day for 30 minutes each time.
5) Burying soil and producing ganoderma lucidum: placing the lychee basswood bag which is fully covered with hyphae into a furrow of a greenhouse, covering soil on the lychee basswood, and culturing for 30-40 days to obtain fruiting bodies, wherein the temperature of the greenhouse is kept between 24-27 ℃ and the air humidity is kept between 70-90%, and the pH value of soil is controlled between 5 and 6 in the culturing process. In this example, it is preferable that the temperature of the greenhouse is maintained at 27℃and the air humidity is maintained at 90%, and the pH of the soil is controlled at pH6.
6) And (3) modeling control:
a: and (3) after the mycelium is differentiated, the mycelium is in a bud stage, at the moment, the greenhouse environment is cooled to 16-20 ℃, the fruiting body is irradiated by adopting a light source, and the light source is vertically arranged right above the fruiting body during irradiation, and the illumination intensity is controlled to be 400-900 Lux for 13-16 days so as to directionally culture the ganoderma atrum in a bud bloom shape. In this example, it is preferable that the temperature of the greenhouse environment is lowered to 20℃after the mycelium is differentiated, and the illumination intensity is controlled to 900Lux for 16 days during irradiation.
Further, the light source is an LED light source, the distance between the LED light source and the fruiting body is 40-70 cm, and the LED light source is turned off in a night state. In this embodiment, the distance between the LED light source and the fruiting body is preferably 70cm.
b: increasing the illumination intensity to 1800-3600 Lux, controlling the air humidity to be 80-95%, introducing carbon dioxide into the greenhouse, controlling the carbon dioxide concentration in the greenhouse to be 0.1-0.3%, lasting for 10-15 days, cutting a V-shaped groove on a blade or a ganoderma lucidum handle of a fruiting body when the length of the ganoderma lucidum handle is 6-13 cm, cutting the bottom of a ganoderma lucidum fungus foot to be grafted into a sharp block matched with the V-shaped groove, fixing the sharp block of the ganoderma lucidum fungus foot to the V-shaped groove of the grafted fruiting body by using a bracket, and dismantling the bracket after healing of a grafted wound. In this example, it is preferable to increase the illumination intensity to 3600Lux, control the air humidity to be 95%, and introduce carbon dioxide into the greenhouse to control the carbon dioxide concentration in the greenhouse to be 0.3%, for 15 days, when the length of the stem is as long as 13cm, a V-shaped groove is cut on the leaf or stem of the fruiting body, and the depth of the V-shaped groove is 3mm.
Further, after the ganoderma lucidum is grafted and survived, the ganoderma lucidum with the edge of the ganoderma lucidum cover being differentiated is arranged in the opposite direction, and illumination with the illumination intensity of 1500-2000 Lux is given to the back surface of the ganoderma lucidum cover for 3-4 days until new primordia are differentiated from the back surface of the illuminated ganoderma lucidum cover, and the primordia can be continuously differentiated along the ganoderma lucidum cover, so that the ganoderma atrum with multi-face ganoderma lucidum covers is formed. In this example, it is preferable to apply light with an intensity of 2000Lux to the back surface of the cover for 4 days.
c: after the ganoderma lucidum is grafted to survive or the ganoderma atrum main body is basically formed, the control temperature in the greenhouse is kept at 23-26 ℃, the air humidity is kept at 80-90%, the illumination intensity is controlled at 1500-2500 Lux, and the pH value of soil is controlled at pH6. In this example, it is preferable to control the temperature in the greenhouse to 26℃and the air humidity to 90% and the light intensity to 2500Lux.
d: after the black flower ganoderma lucidum grows and matures, the cultivation bag sleeved outside the lychee basswood can be removed, the surface of the lychee basswood is kept dry, and the shaped black flower ganoderma lucidum is subjected to local trimming and then is harvested.
Compared with the prior art, the invention has the following beneficial effects:
1. the cultivation method of the ganoderma atrum disclosed by the invention is different from the traditional technology, the ganoderma atrum is molded by adopting an induction type process, natural conditions such as illumination intensity, light direction, temperature and humidity and the like which influence the growth of the ganoderma lucidum are changed in different growth periods of the ganoderma lucidum through artificial regulation, so that the ganoderma lucidum presents an artistic model with beautiful flower shape in the growth process, the whole process is natural culture, the finished product manufacturing process is free of painting and adding any chemical component, no toxic component is added, and the product can be stored for many years and has the same color as the first year;
2. the cultivation method of the ganoderma atrum disclosed by the invention is simple in process and easy to operate, and the survival rate of the ganoderma atrum can be effectively improved by adding concentrated nutrition supply, so that the cultivated ganoderma atrum fruiting body is rapid in growth and strong in flower, and has high medicinal value;
3. the ganoderma atrum cultivated by the invention has beautiful appearance and strong ornamental value, and highlights the spirit and lucky meaning of ganoderma atrum, can be applied to the decoration of high-grade rooms, offices and hotels, and greatly improves the ornamental value and economic benefit of ganoderma atrum.
The foregoing description of the preferred embodiment of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (4)
1. A method for cultivating ganoderma atrum by utilizing illumination is characterized in that: the method comprises the following steps:
1) Preparation of a culture medium: the culture medium comprises the following raw materials in percentage by mass: wood chips 35%, wheat bran 45%, corn flour 15%, quartz 3% and brown sugar 2%, and adding water into the raw materials of the culture medium for uniform mixing to obtain the culture medium;
2) Basswood manufacturing: selecting lychee wood as a cultivation material, cutting the lychee wood into basswood with the length of 20-30 cm, drying under natural conditions until the water content of the lychee basswood is reduced to 30-35%, sleeving the lychee basswood and a culture medium into a cultivation bag, and then sterilizing;
3) Inoculating: after sterilization, inoculating ganoderma atrum strain into the cultivation bag in a sterile environment;
4) Hypha culture: transferring the cultivation bag into a cultivation room for cultivation after inoculation, keeping darkness in the cultivation room, controlling the temperature at 23-26 ℃ and the air humidity at 55-65%, and ventilating 3-4 times per day for 20-30 min each time until mycelia are fully distributed on the surface layer of the lychee basswood;
5) Burying soil and producing ganoderma lucidum: placing the lychee basswood full of mycelia in furrows of a greenhouse in a bag-removing manner, covering soil on the lychee basswood, and culturing for 30-40 days to obtain fruiting bodies, wherein the temperature of the greenhouse is kept between 24-27 ℃ and the air humidity is kept between 70-90%, and the pH value of soil is controlled between 5 and 6;
6) And (3) modeling control:
a: the method comprises the steps that after the mycelium is differentiated, the mycelium is in a bud stage, at the moment, the greenhouse environment is cooled to 16-20 ℃, a light source is adopted to irradiate the fruiting body, the light source is vertically arranged right above the fruiting body during irradiation, the illumination intensity is controlled to be 400-900 Lux, the illumination lasts for 13-16 days, ganoderma atrum in a bud blooming shape is directionally cultivated, the light source is an LED light source, the distance between the LED light source and the fruiting body is 40-70 cm, and the LED light source is turned off during a night state;
b: increasing the illumination intensity to 1800-3600 Lux, controlling the air humidity to be 80-95%, introducing carbon dioxide into the greenhouse to control the carbon dioxide concentration in the greenhouse to be 0.1-0.3%, lasting for 10-15 days, cutting a V-shaped groove on a blade or a ganoderma lucidum handle of a fruiting body when the ganoderma lucidum handle is 6-13 cm long, cutting the bottom of a ganoderma lucidum fungus foot to be grafted into a sharp block matched with the V-shaped groove, fixing the sharp block of the ganoderma lucidum fungus foot into the V-shaped groove of the grafted fruiting body by using a bracket, and dismantling the bracket after healing of a grafted wound;
c: after the ganoderma lucidum is grafted to survive or the ganoderma atrum main body is basically formed, the control temperature in a greenhouse is kept between 23 ℃ and 26 ℃, the air humidity is kept between 80% and 90%, the illumination intensity is controlled between 1500 and 2500Lux, the pH value of soil is controlled at pH6, after the ganoderma lucidum is grafted to survive, the ganoderma lucidum with the edge of the ganoderma lucidum cover being differentiated is arranged in the opposite direction, illumination with the illumination intensity of 1500-2000 Lux is given to the back surface of the ganoderma lucidum cover for 3-4 days until new primordium is differentiated from the back surface of the illuminated ganoderma lucidum cover, and the primordium can be continuously differentiated along the ganoderma lucidum cover, so that the ganoderma atrum with multiple faces of ganoderma lucidum covers is formed;
d: after the black flower ganoderma lucidum grows and matures, the cultivation bag sleeved outside the lychee basswood can be removed, the surface of the lychee basswood is kept dry, and the shaped black flower ganoderma lucidum is subjected to local trimming and then is harvested.
2. The method for cultivating ganoderma atrum by using illumination as claimed in claim 1, wherein: the pH value of the culture medium is 6, and the water content of the culture medium is 60-65%.
3. The method for cultivating ganoderma atrum by using illumination as claimed in claim 1, wherein: the cultivation bag is made of polyethylene.
4. The method for cultivating ganoderma atrum by using illumination as claimed in claim 1, wherein: the sterilization treatment is a normal pressure sterilization or high pressure sterilization treatment method;
the normal pressure sterilization treatment method comprises the following steps: placing the cultivation bag in a sterilizing cabinet, wherein the temperature in the sterilizing cabinet is 100 ℃, and the sterilizing time is 4-5 hours;
the high-pressure sterilization treatment method comprises the following steps: placing the cultivation bag in an autoclave, and continuously heating in the autoclave until the pressure in the autoclave rises to 0.11-0.13 MPa, and stopping heating after keeping for 1.5-3 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110365108.0A CN115176641B (en) | 2021-04-01 | 2021-04-01 | Method for cultivating ganoderma atrum by utilizing illumination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110365108.0A CN115176641B (en) | 2021-04-01 | 2021-04-01 | Method for cultivating ganoderma atrum by utilizing illumination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115176641A CN115176641A (en) | 2022-10-14 |
| CN115176641B true CN115176641B (en) | 2023-08-01 |
Family
ID=83512018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110365108.0A Active CN115176641B (en) | 2021-04-01 | 2021-04-01 | Method for cultivating ganoderma atrum by utilizing illumination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115176641B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688750A (en) * | 2013-12-16 | 2014-04-02 | 福建农林大学 | Manufacture method of living body lucid ganoderma bonsai |
| CN104115673A (en) * | 2014-08-08 | 2014-10-29 | 江西省农业科学院农业应用微生物研究所 | Ornamental ganoderma lucidum cultivation method |
| CN105379557A (en) * | 2015-11-09 | 2016-03-09 | 巫溪县云祥食用菌股份专业合作社 | Method for conducting living body graft on lucid ganoderma bonsai through cut log |
| CN107079709A (en) * | 2017-03-28 | 2017-08-22 | 云南顶兴农业科技发展有限公司 | A kind of ganoderma lucidum breeding method for viewing and admiring |
-
2021
- 2021-04-01 CN CN202110365108.0A patent/CN115176641B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688750A (en) * | 2013-12-16 | 2014-04-02 | 福建农林大学 | Manufacture method of living body lucid ganoderma bonsai |
| CN104115673A (en) * | 2014-08-08 | 2014-10-29 | 江西省农业科学院农业应用微生物研究所 | Ornamental ganoderma lucidum cultivation method |
| CN105379557A (en) * | 2015-11-09 | 2016-03-09 | 巫溪县云祥食用菌股份专业合作社 | Method for conducting living body graft on lucid ganoderma bonsai through cut log |
| CN107079709A (en) * | 2017-03-28 | 2017-08-22 | 云南顶兴农业科技发展有限公司 | A kind of ganoderma lucidum breeding method for viewing and admiring |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115176641A (en) | 2022-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103688750B (en) | A kind of preparation method of live body glossy ganoderma dish garden | |
| CN102090267B (en) | Method for cultivating mulberry twig glossy ganoderma | |
| KR102013799B1 (en) | Cultivation method of mushrooms by supplying nutrient and controlling growth environment to need mushroom growth | |
| CN103598018A (en) | Method for producing mushroom fungus brick by taking wood chips as main materials and cultivating mushroom fungus brick | |
| CN102863273B (en) | Field cultivating black fungus culture medium in Tibet region and cultivation method for field cultivating black fungus in Tibet region | |
| KR101423983B1 (en) | Production method of high quality Medium for cultivating a mushroom using them | |
| CN1545839A (en) | Cultivation method of alive Glossy Ganoderma potted landscape | |
| CN103125274B (en) | Grafting method between different types of ganoderma lucidum and potted landscape process | |
| CN103026897A (en) | Lucid ganoderma bonsai grafting method | |
| KR100349579B1 (en) | Method for cultivating a paecilomyces japonica | |
| CN115176641B (en) | Method for cultivating ganoderma atrum by utilizing illumination | |
| CN105191736B (en) | A kind of breeding method for seeing the dual-purpose Moringa potted landscape of food | |
| CN104488559A (en) | Manufacturing method of lucid ganoderma bonsai | |
| CN115152525B (en) | Method for cultivating ganoderma lucidum by utilizing illumination | |
| CN106718067A (en) | A kind of section of planting patterns of wooden white fungus | |
| KR100786744B1 (en) | Culture Medium and Cultivation Method for Lyophyllum decastes | |
| CN109511462A (en) | A kind of glossy ganoderma dish garden process for making | |
| CN105145303B (en) | A kind of method that pure sandy soil nursery fast induces rice blast | |
| CN105379557A (en) | Method for conducting living body graft on lucid ganoderma bonsai through cut log | |
| CN109348989A (en) | The efficient stable yields cultural method of white ginseng bacterium | |
| Chen | Natural log cultivation of the medicinal mushroom, Ganoderma lucidum (Reishi) | |
| CN103503692A (en) | Wild trametes orientalis Imaz domestication cultivation technology | |
| CN106105781A (en) | A kind of glossy ganoderma dish garden engrafting method | |
| CN112931032A (en) | Cultivation method of living lucid ganoderma bonsai | |
| CN112913576A (en) | Cultivation method of black-skin termitomyces albuminosus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |