KR100349579B1 - Method for cultivating a paecilomyces japonica - Google Patents

Abstract

The present invention relates to a method for cultivating Cordyceps sinensis (冬蟲夏草), comprising the steps of preparing a cordyceps culture medium, sterilizing the prepared culture medium, inoculating the seedlings of Cordyceps sinensis, culturing the inoculated culture medium with Cordyceps mycelium in the culture room, and In the Cordyceps cultivation method comprising growing a culture medium in which the mycelium is cultured as Cordyceps fruiting body in the cultivation room, in preparing the culture medium, sugar or chitosan may be selectively added together with distilled water and boiled water. A medium containing a small amount of agar was infiltrated into the cordyceps host so that the moisture content of the cordyceps host was 65 to 75%, and Pecilomomyces japonica (Paecilomyces japonica) cultured in the step of inoculating the cordyceps fungi strain or cordyceps. Contaminated Militaris (Cordyceps militaris) in a liquid spawn state In the culture conditions in the mycelium culture step is cultured for 10 to 15 days in a well-ventilated state at a temperature of 18 ~ 22 ℃, in the fruiting body growth step, the mycelia were cultured in the body of the Cordyceps sinensis host with an additional culture solution Next, it is grown in a culture container or grown with the cordyceps host on a skewer, and the growth conditions of fruiting bodies are grown differently during the germination period for 4-5 days and during the growth period for 25-40 days from the start of growth. Provides a cordyceps cultivation method.

Description

Cordyceps cultivation method {METHOD FOR CULTIVATING A PAECILOMYCES JAPONICA}

[Industrial use]

The present invention relates to a cultivation method of Cordyceps sinensis (冬蟲夏草), and more particularly, inoculated with the Peony omycete japonica or Cordyceps militaris bacteria in dried silkworm larva or pupa. After forming a fruiting body in the silkworm larva or pupa infected with the bacteria, it relates to a method of artificially cultivating silkworm snowworms, pupa snowworms, silkworm millitaris, and pupa millitas.

[Prior art]

Cordyceps sinensis is a small mushroom belonging to the genus Cordyceps sinensis in the Aspergillus fungus, and its name is derived from the formation of mushrooms in the body of insects, which are parasitic in winter and host in summer. . To explain the formation process of the Cordyceps Sinensis in more detail, Cordyceps Sinensis or Conidia spores are formed to invade the soft parts such as respiratory organs, digestive organs, and joints of living insects, The insects die by forming mycelia while nutrients are consumed, and then in the summer, fruiting bodies are formed using the nutrients of the host insect.

These insects include Cordyceps sinensis, Pupa Cordyceps militaris, Cordyceps scarabaeicola, Cordyceps mutans, Beetle Cordyceps japonica japonica p. Etc.

Among them, the medicinal effect has been recognized from ancient times, Cordyceps sinensis, or it is known that artificial cultivation is difficult because it is collected only in the snow line alpine area of more than 4000m above sea level. In addition, windworms, insects, insects, and insects are not suitable for use because their host is an aversive insect. Therefore, artificial cultivation has been studied only for Cordyceps militaris and Paecilomyces japonica, which are mainly silkworm or silkworm pupae.

Cordyceps sinensis is somewhat different in its composition according to its type, but is generally composed of moisture, fat, crude protein, carbohydrate and ash, and protein contains about 18 kinds of essential amino acids which are indispensable to the human body. In addition, Cordyceps sinensis contains special ingredients such as Cordyceps Polysaccharides and Cordycepic Acid, and has been known as an elixir of indolence as well as for treating pulmonary tuberculosis, asthma, anemia, fatigue and hypertension. Recently, it has been proved that Cordyceps sinensis contains ingredients that enhance immune function and anti-cancer ingredients, and research is being actively conducted, and it is effective as an antidote for drug addiction and effective pest control because of its parasitic properties on insects. It is also very useful as a biopesticide that can prevent environmental pollution.

In spite of such excellent effect, it is very difficult to collect Cordyceps fruiting body grown in a natural state, so artificial cultivation technology for stably supplying Cordyceps fruiting body is required.

The artificial cultivation method of Cordyceps sinensis so far is based on the fact that Cordyceps sinensis forms fruiting bodies based on insects. It mainly inoculates the larvae or silkworm larvae with Cordyceps militaris and Paecilomyces japonica. It is about producing fruiting bodies.

There is a method for cultivating Cordyceps sinensis Korean Patent Publication No. 1998-000013. In the present invention, insects, in particular silkworm pupa are put in water and boiled, and then the water content is adjusted to 60-65%, put into a spawn bottle and sterilized. A method of culturing a seed is disclosed.

Another prior art is Korean Laid-Open Patent Publication No. 1998-023907. The present invention discloses a method of culturing fruiting bodies by inoculating silkworm 4-5 larvae or pupa to Paecilomyces japonica.

Another prior art is Korean Laid-Open Patent Publication No. 1998--33558. The present invention discloses a method of culturing fruiting bodies by inoculating Cordyceps sinensis liquid seedlings so that the whole surface of the medium can be grown in a state where the whole surface of the medium is grown using a medium in which pupa is added to the grain medium.

However, in the case of Cordyceps sinensis, although it is a kind of mushroom, it grows in a more difficult growth condition than other mushrooms, unlike the expectation that it will occur under conditions similar to those of ordinary mushrooms. Therefore, when the cordyceps is cultivated according to the growth conditions disclosed in the above invention, it is possible to harvest cordyceps, but the yield and ingredients are questionable and there is a problem in mass production. have.

An object of the present invention is to solve the problems of the prior art as described above, an object of the present invention is to produce fruiting bodies by inoculating pupa Cordyceps militaris and Paecilomyces japonica bacteria in silkworm larvae or silkworm pupa However, it is to provide a cultivation method of Cordyceps sinensis that can be mass-produced and to facilitate the handling of cultivated Cordyceps sinensis by precisely adjusting the cultivation conditions.

1 is a perspective view showing a culture vessel used for culturing and growing Cordyceps sinensis of the present invention,

Figure 2 is a perspective view showing one of the state in which the cordyceps host cultured mycelium cultured in the present invention arranged in the cultivator,

Figure 3 is a photograph of silkworm snow Cordyceps sinensis cultivated according to an embodiment of the present invention,

Figure 4 is a photo of pupa snowflower Cordyceps sinensis cultivated according to an embodiment of the present invention,

Figure 5 is a photograph of pupa militas Cordyceps sinensis cultivated according to an embodiment of the present invention.

[Means for solving the problem]

In order to achieve the above object, the present invention comprises the steps of preparing a Cordyceps sinensis culture medium, sterilizing the prepared culture medium and inoculating the seedlings of Cordyceps sinensis, culturing the inoculated culture medium with Cordyceps mycelium in the culture chamber and mycelium cultured In the Cordyceps cultivation method comprising the step of growing the culture medium as Cordyceps fruit fruit in the cultivation room, preparing the culture medium in the step of adding the sugar or chitosan to distilled water and boiled potatoes selectively or together with a trace of agar The added medium solution is soaked into the cordyceps host so that the moisture content of the cordyceps host is 65-75%, and the Peciomyomyces japonica or Cordyceps millitaris cultured at the step of inoculating the Cordyceps spp. Cordyceps militaris) and inoculated in liquid spawn state, the hyphae The culture conditions in the culture step is incubated for 10 to 15 days in a well-ventilated state at a temperature of 18 ~ 22 ℃, in the fruiting body growth step, the Cordyceps sinensis host in which mycelium is cultured in the body is applied as an additional culture medium, the culture vessel Cordyceps cultivation, characterized in that it is grown in the state or the cordyceps host host is skewered and grown under different conditions during the germination period for 4-5 days and the growth period for 25-40 days from the start of growth. Provide a method.

The present invention relates to a method of cultivating Cordyceps sinensis, which has been established through many years of trial and error in a general farm, and the present invention will be described in detail as follows.

The present invention is a silkworm larvae inoculated Paecilomyces japonica bacteria silkworm larvae and silkworm pupae inoculated Paecilomyces japonica (Pecilomyomyces japonica), and silkworm larvae Cody It relates to four types of cordyceps cultivation methods: silkworm Militaris cordyceps inoculated with Cordyceps militaris and pupa Militas cordyceps inoculated with Cordyceps militaris in silkworm pupa.

As a species of Cordyceps sinensis used in the present invention, the bacterium Paecilomyces japonica and Cordyceps militaris were used as liquid seed stocks sold from Korea Cordyceps Sinensis (KEFC).

Here, the species of Cordyceps sinensis may be used as the solid seed, but the liquid seed is used because the seed is inoculated on the surface of the culture medium and it takes a lot of time to grow the mycelium and grow well. Liquid spawn production method is described in Republic of Korea Patent Publication Nos. 97-27294 and 97-27295, so the detailed description thereof will be omitted.

As a culture medium for culturing Cordyceps sinensis, prepare a medium solution containing sugar or chitosan selectively or together with distilled water and boiled water, and then add a trace of agar to the prepared medium solution. After dipping or mixing the cultured medium and cordyceps host, it is maintained at room temperature for 11 to 13 hours, preferably around 12 hours, so that the medium solution penetrates into the cordyceps host and the moisture content is 65. To 75%.

Here, the silkworm larva is immersed in the medium solution because its own moisture is not high, but in the case of silkworm pupae, when thawed using the frozen pupa, the content of its own water is about 60%, so it is possible to maintain the necessary moisture only by mixing with the medium solution. have. And controlling the content of water to 65 to 75% is less than 65% of the mycelium cultivation is good, but the fruiting body does not germinate, when more than 75% fruiting body germination is good, but the mycelium is not well cultured. Therefore, keeping the moisture as low as possible within the well-cultivated range of mycelium is advantageous for growing good Cordyceps sinensis.

The silkworm larva used in the present invention was selected from 5 days 3 days or 5 days 6 days silkworms weighing 5-6g. Table 1 shows the chemical composition of silkworm 5 days 3 days or 5 days 6 days used in the present invention.

[Table 1] Chemical composition of silkworms by stages of development

As shown in Table 1, carbohydrates were compared to the chemical composition of 5-day-old silkworm silkworm, Cordyceps sinensis (10.84% fat, 8.4% fat, 25.32% crude protein, 28.9% carbohydrate, and 4.1% ash). It is most similar to the chemical composition of wild bat moth cordyceps. Here, in case of 5-year-old silkworms, insufficient carbohydrates can be replaced with extra crude protein contained in 5-year-old silkworms by adding sugar or chitosan as a carbon source. It is also possible to add soy flour as a nitrogen source. Therefore, the culture medium suitable for artificial cultivation of Cordyceps sinensis is most preferably culture medium in which sugar is added to silkworms 5 days and 3 days old.

In addition, the silkworm used in the present invention has a feature in that the silkworm in the living state is not directly used, but is used in a dead state by hot air drying the silkworm. The silkworms are dried in hot air and added to the culture medium in order to shorten the cultivation time and increase the cultivation yield. That is, if the larvae of live silkworms are used as they are, the silkworm larvae inoculated with the spawn grow and strike the silkworms, and after 15 days of incubation, they must be picked up by picking cocoons to breed the mycelia, but in the present invention and Using the silkworm larva in a hot air dry state not only saves the time for silkworms to repair cocoons, but also eliminates the need to screen the cocoons for breeding mycelium.

Here, the conditions for drying the silkworms in a hot air drying in a conventional dryer, but less than 50 ℃ not only takes a long time, but also may not be completely dried, if the 80 ℃ or more may destroy the nutrients of silkworms 50 ~ 80 ℃, Especially preferably, 60-70 degreeC is suitable. Hot air drying time is 3 ~ 4 days is enough, and the silkworm to be dried is changed from white to dark blue.

About 120 g of wet Cordyceps hosts are injected into a 500cc polypropylene (PP) container to incubate Cordyceps hosts with a water content of 65 to 75%. The PP culture vessel used here used a container as shown in FIG. 1 with no bottleneck and a wide inlet in order to facilitate separation of Cordyceps sinensis grown after incubation.

The culture vessel 10 containing the wet cordyceps host host is put into a sterilization chamber at a high temperature and high pressure in a closed state with a bottle cap 20 and sterilizes various fungi in the culture medium. At this time, the temperature of the sterilization chamber is about 120 ℃ and the sterilization time is about 1 hour 30 minutes to 2 hours.

Move the semi-cultured culture medium to the inoculation chamber, open the bottle cap 20, and then spray the pre-cultured liquid spawn using an air compressor and shake the culture vessel 10 at the same time so that the liquid spawn is evenly inoculated on the cordyceps host culture medium. . At this time, the inoculation chamber is sterilized with phenol and maintains aseptic condition, and after inoculating the liquid spawn, the bottle cap 20 is immediately closed.

A large amount of culture medium inoculated with the liquid spawn prepared by the above method is transferred to the culture chamber to culture the mycelia. The cultivation chamber is shielded from sunlight and has a multi-stage pedestal, and the temperature is maintained at 18-22 ° C. and incubated for 10 to 15 days under good ventilation. The liquid spawn inoculated on the epidermis of silkworm larva or pupa during the cultivation stage germinates when the spores attached to the epidermis meet conditions such as temperature and humidity, and penetrates the epidermis of the respiratory and digestive organs of Cordyceps sinensis. Growth and growth in the state is filled with mycelia in the body.

As described above, the culture medium in which the mycelium is sufficiently cultured in the body is moved to the grower to grow fruiting bodies. In this case, it is another feature of the present invention that the cultured mycelium cultured Cordyceps sinensis host is not transferred to a grower as it is, but is grown in a grower after the culture solution in which sugar and trace chitosan is dissolved in distilled water is applied to the Cordyceps sinensis host once again. The culture medium used herein is preferably used by dissolving 10 kg of sugar and 0.5 g of chitosan in 1000 cc of distilled water. When the silkworm larvae are based, they are lightly immersed in the culture medium and immediately taken out. Sprinkle with and apply.

In the present invention, the culture vessel itself is placed in a cultivator to grow fruiting bodies in the culture vessel, and the method of growing fruiting bodies by placing the Cordyceps sinensis cultivated mycelia on the cultivator as shown in Fig. 2 on a wooden skewer. Used. The growth of the Cordyceps sinensis cultured with mycelium on a wooden skewer is intended to allow for the handling of the Cordyceps sinensis after cultivation. It is appropriate to attach about 7 to 10 Cordyceps sinensis hosts to one skewer.

The grower used in the present invention is a conventional grower in the form of a house or a vinyl house, in which two shelves are arranged. The culture vessel placed in the cultivator is placed on the shelf with 20 to 30 culture vessels on one bacterium with the lid open, and covered with vinyl outside the shelf. In the case of using wooden skewers, the wooden skewers are vertically attached to the straw foam laid on the shelf or horizontally attached as shown in FIG.

In the prepared state as above, the growth process of cordyceps fruiting body begins.

In the case of general edible mushroom cultivation, as the growth environment is different depending on the type of mushroom, the environment required to grow Cordyceps sinensis also differs from that of the general edible mushroom. Therefore, the germination and growth of fruiting bodies of Cordyceps sinensis should be controlled to the appropriate temperature, humidity and light.

The temperature at which Cordyceps sinensis can germinate is less than 18 ° C and 23 ° C or higher, so 18-23 ° C is appropriate, and the optimum temperature necessary for the growth of Cordyceps is 22-26 ° C. And germination time is suitable for 4 to 5 days and growth time is preferably 25 to 40 days from the grower batch date.

In addition, the humidity required to grow Cordyceps sinensis should be maintained at about 95 to 100% higher than ordinary mushrooms.

In the case of Cordyceps sinensis, light is not necessary at all in the mycelium cultivation stage, but the light rays are harmful to mycelial growth. However, in the germination and growth stages of the fruiting body, an appropriate amount of light rays are required, so it is necessary to supply 300-500 lux light to the grower. At this time, the supply of light turns on the lamp installed inside the planter and indirect lighting (diffusion), and the light supplied is irradiated only during the day, and at night, the light is turned off to block the supply of light.

The growth conditions of Cordyceps sinensis as described above should be kept constant throughout the growth period. If the growth conditions are changed and growth is stopped once, the growth is not recovered even if it is reduced to the optimum condition again.

Cordyceps sinensis can be grown and harvested completely after 25 ~ 40 days after the start of growth.

EXAMPLE

The following presents a preferred embodiment to aid the understanding of the present invention. However, the following examples are merely provided to more easily understand the present invention, and the present invention is not limited to the following examples.

Example 1 ( silkworm snow Cordyceps sinensis)

Prepare 5 days 3 days silkworms at 60 ~ 70 ℃ for 3 ~ 4 days with hot air dryer. Separately, prepare 1000-5000cc of medium solution containing 1 kg of sugar and a small amount of agar in boiled water. 5 kg of dried silkworm silkworms are added to the prepared medium solution and soaked for about 12 hours so that the silkworms have a moisture content of 65 to 75%. Then, 120 g of silkworms called the culture medium is placed in a 500 cc translucent polypropylene culture vessel. Many prepared culture vessels are placed in a high temperature autoclave and sterilized at 121 ° C. for 2 hours. Move the sterilized culture vessel to the inoculation chamber and shake the culture vessel evenly by spraying evenly the liquid bacterium Paecilomyces japonica (strain No. KFC-C-660) on the skin of the sterilized silkworm using an air compressor. . Immediately after inoculation of the liquid spawn, the lid is closed and transferred to the incubation chamber. The culture is incubated at 18-22 ° C. for 10 days. After cultivating the silkworm, open the lid of the culture vessel, put 7-8 silkworms on a wooden skewer, and soak 10kg of sugar and 0.5g of chitosan in a culture solution of 1000cc of distilled water. Skewers of silkworms with mycelium formed are placed in the cultivator, partly vertically and partly horizontally. The temperature of the grower is maintained at 20-21 ℃, the humidity is kept at 95-100%, the lights are turned on during the day to supply 300-500 lux and the lights are turned off at night to keep the light off. Germinate fruiting bodies for 5 days. After confirming that the fruiting body germinated, the temperature of the grower is lowered to 18 to 20 ℃ and the humidity is maintained at 95% or more after 25 to 28 days from the start of the cultivation Cordyceps fruit fruit will be able to grow and harvest. The silkworm Snow Cordyceps sinensis cultivated by the above cultivation method is shown in FIG.

Example 2 ( pupa snowflower Cordyceps sinensis)

Prepare a medium solution containing 1 kg of sugar, 0.2 g of powdered chitosan, and a small amount of agar in 300-500 cc of boiled water. The prepared medium solution and 10kg of chrysalis of frozen chrysalis containing 65% to 75% of water are mixed, and then 120g of chrysalis are put into a 500cc translucent polypropylene culture vessel. The sterilization process, the inoculation process and the incubation process of the liquid spawn and the liquid spawn used were the same as in Example 1. The cultured chrysalis are sprayed with a sprayer of a culture solution in which 10 kg of sugar and 0.5 g of chitosan are dissolved in 1000 cc of distilled water with the lid of the culture vessel open, and then the culture vessel is placed in a cultivator. The temperature of the grower is maintained at 22 to 23 ℃, the humidity is maintained at 95% or more germination of the fruiting body, after confirming that the fruiting body is completely germinated, and then again the temperature to 23 to 25 ℃ and the humidity to 95 to 100% Grown fruiting bodies in a maintained state. At this time, the light supply conditions are the same as in Example 1, 28 ~ 30 days after the start of the cultivation of Cordyceps sinensis fruit grows and can be harvested. Fig. 4 shows the chrysalis of the chrysalis cultivated by the above cultivation method.

Example 3 Silkworm Militaris Cordyceps Sinensis

Prepare 5 days 3 days silkworms at 60 ~ 70 ℃ for 3 ~ 4 days with hot air dryer. Separately, prepare 1000-5000 cc of medium solution containing 1 kg of sugar, 0.2 g of powdered chitosan, and a small amount of agar. 5 kg of dried silkworm silkworms are added to the prepared medium solution and soaked for about 12 hours so that the silkworms have a moisture content of 65 to 75%. Then, 120 g of silkworms called the culture medium is placed in a 500 cc translucent polypropylene culture vessel. Many prepared culture vessels are placed in a high temperature autoclave and sterilized at 121 ° C. for 2 hours. Move the sterilized culture vessel to the inoculation chamber and shake the culture vessel evenly by spraying the liquid spawn Cordyceps militaris (strain No. KFC-C-738) on the epidermis of the sterilized silkworm using an air compressor. . Immediately after inoculation of the liquid spawn, the lid is closed and transferred to the incubation chamber. The culture is incubated at 18-22 ° C. for 10-15 days. After cultivating the silkworm, open the lid of the culture vessel, put 7-8 silkworms on a wooden skewer, and soak 10kg of sugar and 0.5g of chitosan in a culture solution of 1000cc of distilled water. Silk skewers that have mycelium formed on them are placed vertically on the cultivator's ground, and partly horizontally. The temperature of the grower is maintained at 20-22 ℃, the humidity is kept at 95-100%, the lights are turned on during the day to supply 300-500 lux of light, and the lights are turned off at night to keep the light off. After 5 days, fruiting bodies germinate. After confirming the germination of the fruiting body, the temperature of the grower to 25 ~ 26 ℃ and the humidity is maintained at 95% or more, after 30-40 days from the start of cultivation, Cordyceps fruiting body grows and can be harvested.

Example 4 Pupa Militas Cordyceps sinensis

Prepare a medium solution containing 1 kg of sugar, 0.2 g of powdered chitosan, and a small amount of agar in 300-500 cc of boiled water. The prepared medium solution and 10kg of chrysalis of frozen chrysalis containing 65% to 75% of water are mixed, and then 120g of chrysalis are put into a 500cc translucent polypropylene culture vessel. The sterilization process, the inoculation process and the incubation process of the liquid spawn and the liquid spawn used were the same as in Example 3. The cultured chrysalis are sprayed with a sprayer of a culture solution in which 10 kg of sugar and 0.5 g of chitosan are dissolved in 1000 cc of distilled water with the lid of the culture vessel open, and then the culture vessel is placed in a cultivator. The fruiting body is germinated in a state where the temperature of the grower is maintained at 22 to 23 ° C. and the humidity is maintained at 95% or more. After confirming that the fruiting body is completely germinated, the fruiting body is grown while the temperature of the grower is raised to 25 to 26 ° C. and the humidity is maintained at 95 to 100%. At this time, the light supply conditions are the same as in Example 3, 30-40 days after the start of the cultivation, Cordyceps sinensis fruit grows and can be harvested. The pupa Militas cordyceps sinensis cultivated by the above cultivation method is shown in FIG.

The present invention is inoculated to pupa larvae or silkworm pupae in 5 days 3 days or 5 days 6 days of hot air dried Cordyceps militaris and Paecilomyces japonica bacteria to produce fruiting bodies, but precisely the conditions for cultivation such as temperature and humidity Can control mass production and can grow cultivated cordyceps by easily growing cultivated fruiting body by inserting cultured silkworms into tree trunks.

Claims (11)

Preparing a Cordyceps sinensis medium, sterilizing the prepared culture medium, inoculating the seedlings of Cordyceps sinensis, culturing the inoculated culture medium into Cordyceps mycelium in the culture chamber, and growing the culture medium in which the mycelia were cultured as Cordyceps fruiting body in the cultivation room. In the Cordyceps cultivation method consisting of steps, Moisture content of Cordyceps sinensis by distilled water and potato boiled water, optionally or together with sugar or chitosan, and the medium solution containing trace amount of agar infiltrated into Cordyceps sinensis host consisting of 5 days 3 days silkworm or 5 days 6 days silkworm Preparing a culture medium so as to be 65 to 75%; Wow Inoculating the prepared culture medium with liquid seed of Pecilomyces japonica (Paecilomyces japonica); Wow Culturing the mycelia for 10-15 days in a well-ventilated state at a temperature of 18-22 ° C. inoculated with the Cordyceps fungus; Wow Preparing mycelia to grow fruiting bodies by applying Cordyceps sinensis cultured in the body to the culture medium; Wow Maintaining the cordyceps herb host at 95 to 100% humidity at a temperature of 20 to 21 ° C., and germinating fruiting bodies for 4 to 5 days; And Growing Cordyceps herb germinated fruiting body for 18 to 20 ℃ for 25 to 40 days while maintaining the temperature of 95 to 100%; Cordyceps cultivation method comprising growing the fruiting body under conditions that supply light of 300 to 500 lux during the germination and growth period during the day of germination and growth. (delete) The cordyceps cultivation method according to claim 1, wherein the 5th and 3rd day silkworms or the 5th and 6th day silkworm cordyceps host strains are used to dry the silkworms for 3 to 4 days with hot air at 50 to 80 ° C. (delete) The method according to claim 1, wherein the growth preparation step, the cordyceps host with the culture solution is prepared in the culture vessel or by placing a plurality of cordyceps host skewer on the skewer, when the cordyceps host host on the skewer a plurality Cordyceps cultivation method of the caterpillar herb host and the skewer arranged vertically to the cultivator, or arranged by tying a plurality of horizontally. (delete) delete Preparing a Cordyceps sinensis medium, sterilizing the prepared culture medium, inoculating the seedlings of Cordyceps sinensis, cultivating the inoculated culture medium into Cordyceps mycelium in the culture chamber, and growing the culture medium in which the mycelia were cultured as Cordyceps fruiting body in the cultivation room. In the Cordyceps cultivation method consisting of steps, Distilled water and potato boiled water were selectively or together with sugar or chitosan, and a small amount of agar was added to the medium of Cordyceps sinensis, which is composed of 5 or 3 days of silkworm or 5 days or 6 days, so that the moisture content of cordyceps is 65 Preparing a culture medium so as to be ˜75%; Wow Inoculating the prepared culture medium with Cordyceps militaris liquid spawn; Wow Culturing the mycelia for 10-15 days in a well-ventilated state at a temperature of 18-22 ° C. inoculated with the Cordyceps fungus; Wow Preparing mycelia to grow fruiting bodies by applying Cordyceps sinensis cultured in the body to the culture medium; Wow Maintaining the cordyceps herb host at 95 to 100% humidity at a temperature of 20 to 22 ° C., and germinating fruiting bodies for 4 to 5 days; And Growing the cordyceps germinated host fruiting body at 25-26 ° C. for 25-40 days while maintaining 95-100% humidity; Cordyceps cultivation method comprising growing the fruiting body under conditions that supply light of 300 to 500 lux during the germination and growth period during the day of germination and growth. delete The method according to any one of claims 1 to 7, wherein the culture solution is Cordyceps cultivation method, characterized in that prepared by dissolving 10kg sugar and 0.5g chitosan in 1000cc of distilled water. Inoculated with a liquid spawn of Paecilomyces japonica on the cordyceps host consisting of 5 or 3 day silkworms or 5 or 6 day silkworms, and after cultivating the same, put the plurality of cordyceps hosts on the skewers, Cordyceps sinensis, characterized in that the cordyceps fruiting body is grown by vertically arranged or arranged in a plurality of horizontally arranged.