KR101133708B1 - Feed for Ruminant Comprising Cordyceps, Breeding Method for Ruminant Using the Same, and a meat produced therefrom - Google Patents

Abstract

The present invention is a method for cultivating Cordyceps sinensis with a cultivation composition of Cordyceps sinensis containing a vegetable raw material having a protein content of more than 50% by weight, feed for ruminants containing Cordyceps cultivated in the same way, the method of breeding ruminants using the same and It relates to the meat, dairy products and processed meat of the ruminant shaft bred by the above method. The meat of the ruminant shaft, which has been bred as a ruminant shaft feed containing the Cordyceps sinensis cultivated by the method of the present invention, has a high content of cordycepin in the muscle, and can be widely used as functional meat, and also used in various meat products and dairy products. Can be.

Militaris Cordyceps Sinensis, Mycelia, Cordycepin, Glatathione Peroxidase

Description

Feed for Ruminant Comprising Cordyceps, Breeding Method for Ruminant Using the Same, and a meat produced therefrom}

The present invention relates to a method of cultivating Cordyceps sinensis with a high content of cordycepin and a feed for a ruminant herb, a method of rearing a ruminant axle, and a meat, a processed product and a dairy product of a ruminant with a high content of cordycepin.

Cordyceps (冬蟲夏草) refers to the cordyceps of Cordyceps spp. Worldwide, which means that it was an insect in winter but turned into a plant in summer. Cordyceps is the origin of the Cordyceps sinensis fruiting body from bat moth larvae in alpine areas of more than 4,000 meters in the Himalayas. Even in high altitudes such as Nepal, Sinensis Cordyceps is collected as a major source of income, and 90% of the Cordyceps is distributed in Hong Kong.

Cordyceps sinensis has been recognized as a potion of immortality to vitalize the human body in China, and recently, Cordyceps genus Cordyceps has been reported to have anticancer activity, fatigue recovery, immunity enhancement, antioxidant activity, hematopoietic effect and antiparasitic effect. have. In addition, Cordyceps sinensis has been reported to have an excellent preventive and therapeutic effect on diseases associated with liver, heart, kidney, lung, etc., which is known to be due to the functional bioactive substances present in Cordyceps sinensis.

Representative physiologically active (functional) substances in Cordyceps sinensis include base-based components such as various nucleosides and nucleosides, polysaccharides composed of galactose and mannose, ergosterol, cordycepic acid, various amino acids and Unsaturated fatty acids and the like. All of these substances may be linked to disease prevention and treatment, but the most dominant functional substance is cordycepin (3'-deoxyadenosine).

On the other hand, Cordyceps sinensis, which has been identified as containing cordycepin, is typically produced by Cordyceps sinensis , which is formed around bat moth larvae, and Cordyceps militaris, which is produced based on silkworm or pupa. There is. The content of cordycepin in these is known to be more distributed in the mycelium than in the fruiting body, and in Militaris species than in the cynosis species. However, it is known that cordycepin is not found in Paecilomyces japonica , which is produced in Korea.

Cordycepin is an analogue of adenine nucleoside, and since there is no hydroxyl group at 3 'position, it acts as a transcription chain terminator, which inhibits RNA synthesis, thereby inhibiting the proliferation of cancer cells. It is used as an anticancer drug to suppress. Cordycepin is particularly effective in treating leukemia. Cell-level studies have shown that it enhances the vitality of natural killer cells and improves the production of interleukin and tumor necrosis factor. It is becoming. It also has the effect of increasing ATP synthesis (enhancing exercise), increasing the titer of superoxide dismutase (SOD) (anti-aging), and reducing blood sugar (diabetes treatment). In addition, it has been reported to show excellent efficacy in the prevention and treatment of infant diarrhea. The structure of cordycepin having the function as described above is represented by the following Chemical Formula 1.

[Formula 1]

On the other hand, in recent years, there has been increasing interest in the method of cultivating mycelium or fruiting body of Cordyceps sinensis which contains a large amount of cordycepin having various functions as described above. In addition, interest in ruminant feed using the same has been increasing. Currently, the cultivation of Cordyceps mycelium or fruiting body is mainly cultivated on the chrysalis, and the cultivated Cordyceps sinensis mycelium is used in the feed. However, the method of using animal hosts such as chrysalis is difficult to use after limiting the use of animal raw materials at home and abroad after the BSE (bovine spongiform encephalopathy) wave, and when using other host materials other than chrysalis, cordycepin Synthetic efficiency is lower than the case of using pupa as a base, so there is a limit to use because it can not see the biological activity.

In addition, the cultivation method based on the chrysalis has problems such as low productivity of Cordyceps mycelia, low content of cordycepin in Cordyceps, high production cost, and unpleasant odor during cultivation. There are many constraints below.

Therefore, the inventors of the present invention have a high content of cordycepin in Cordyceps mycelia and fruiting bodies, high production efficiency, low cost, no odor during production, and suitable for use in feed The present invention was completed while continuing research on the method of cultivating Cordyceps sinensis, the feed for ruminants using the same, and the meat and processed meat and milk products of the ruminants raised by these feeds.

In consideration of the problems of the prior art as described above, the present invention is based on a vegetable raw material having a high protein content, less risk of mad cow disease, etc., cultivation method of cordyceps containing high content of cordycepin, cordyceps cultivated thereby It provides a ruminant feed comprising, a method of rearing the ruminant shaft using the feed and the meat of the ruminant shaft bred thereby, and meat and dairy products thereof.

In order to achieve the above object, the present invention is a method for cultivating cordyceps using cordyceps cultivation composition containing plant material having a protein content of 50% by weight or more, feed for the ruminant herb including cultivated cordyceps, the feed It provides a method of breeding the ruminant shaft using a high-yield ruminant meat of the cordycepin content slaughtered after breeding by the breeding method.

Hereinafter, a method for cultivating Cordyceps sinensis according to a specific embodiment and embodiment of the present invention, a feed for a ruminant shaft comprising Cordyceps cultivated by this, a breeding method of a ruminant shaft using the feed and a ruminant shaft bred by the breeding method Let's describe the meat in detail.

Cordyceps cultivation method according to another embodiment of the present invention is 25 to 30% by weight of the cultivation composition of Cordyceps sinensis containing a vegetable raw material containing a protein content of more than 50% by weight, the moisture content is 70 to 75% by weight for Cordyceps Preparing a medium;

Inoculating the liquid seed of Cordyceps sinensis on the Cordyceps sinensis growth medium;

Culturing the medium inoculated with the liquid spawn to form and grow mycelia and fruiting bodies of Cordyceps sinensis; And

Harvesting Cordyceps sinensis in the medium in which the mycelium and fruiting bodies are formed and grown.

Unlike animal hosts such as pupa, which is a conventional pupa, when the cordyceps is grown on the basis of a medium composition containing a vegetable raw material, the animal host is not used. In addition, there is little odor during the Cordyceps cultivation process, so it is easy to handle and use, and also has a high content of cordycepin in the fruiting body and mycelium of Cordyceps. In particular, when the protein content of the vegetable raw material is more than 50% by weight, the cordycepin content in cordyceps in the cultivated Cordyceps is more than twice as high as the cordyceps grown on the chrysalis. Preferably, soybean protein and corn gluten may be used among vegetable raw materials, in which case, the protein content is 70% by weight or more, and when cordyceps are grown through this, cordycepin content in cordyceps is particularly high, which is ruminant. Cordycepin concentrations in the muscle and blood of the ruminants were also high.

In addition, the water content and the content of the medium composition may be added to 25 to 30% by weight, and the water content may be included 70 to 75% by weight in consideration of the culture period and the culture temperature conditions.

In this case, the isolated soy protein used as a vegetable raw material in the Cordyceps cultivation composition is 25 to 60% by weight of the total vegetable raw material, corn gluten is preferably 40 to 75% by weight. When the content of the vegetable host material in the Cordyceps sinensis cultivation composition within the above range was used, the content of cordycepin in Cordyceps was high, and the content of cordycepin in the muscle and blood of the ruminant axle reared with the feed was also high.

On the other hand, the formation and growth of the mycelium and fruiting body is incubated in the medium inoculated with the liquid species of Cordyceps sinensis for 20 to 30 days at a temperature of 20 to 25 ℃ and the illuminance of 500 to 1200 LUX to form a mycelium of Cordyceps sinensis Making a step; And

The medium in which the mycelium is formed and grown is incubated for 30 to 40 days at a temperature of 19 to 25 ° C., a roughness of 500 to 1200 LUX, and a humidity of 85 to 95% to form and grow a fruiting body of Cordyceps sinensis. desirable.

It specifies conditions optimized for the most vigorous formation and growth of the fruiting body and mycelium of Cordyceps sinensis. By cultivating Cordyceps under these conditions, the harvest time of Cordyceps is reduced, the yield is increased and the content of cordycepin contained in the fruiting body and mycelium. It can contribute greatly to increase.

In addition, the prepared Cordyceps sinensis medium may be sterilized. At this time, the sterilization step is preferably carried out by autoclaving for 30 minutes to 90 minutes at a temperature of 100 to 135 ℃. This is a condition for the most complete sterilization of the medium, and the sterilization step under such conditions, it is possible to remove all bacteria and the like that are disadvantageous for the cultivation of Cordyceps sinensis contained in the medium for cordyceps growth. In other words, by proceeding the subsequent cultivation process in a sterile medium in this way, it is possible to completely prevent the problem that the Cordyceps is contaminated with bacteria or the growth of Cordyceps is suppressed due to such bacteria, so as to improve the quality of the Cordyceps and increase the yield It can contribute greatly.

On the other hand, the inoculation step of the liquid spawn proceeding after the sterilization step is inoculated with the Cordyceps fungi in a flat medium, etc. according to a conventional method known to those skilled in the art, and incubated in a constant temperature period for a period of time to make an inoculator, shaking the inoculator After the proliferation process for a certain period of time, the resultant can be proceeded by homogenizing the resultant homogenizer (homogenizer) to the final production of the liquid seed of Cordyceps sinensis, and inoculating it into the sterile Cordyceps growth medium. The photograph directly taken after the inoculation culture is as shown in FIG. 1.

On the other hand, the present invention also provides a feed for the ruminant herb including Cordyceps sinensis cultivated by the cultivation method according to the present invention.

At this time, Cordyceps sinensis cultivated by the cultivation method according to the present invention may preferably be included 0.1 to 3% by weight of the total feed. When included in less than 0.1% by weight, the content of cordycepin in the muscle, liver or plasma of the ruminant reared in the feed is insignificant and meaningless, if included in more than 3% by weight, with the problem of increasing the cost of the feed ruminant shaft The increase rate of the content of cordycepin contained therein is insignificant.

In addition, Cordyceps sinensis included in the feed may be selected without limitation of the composition as long as it contains cordycepin, preferably mycelia of Militaris cordyceps may be selected. Militaris Cordyceps sinensis has a higher content of cordycepin than cordyceps of other species, and the content of cordycepin in mycelium is higher than that of fruiting bodies.

In addition, the use of the feed may be used for the feeding of ruminants such as sheep, deer, goats, cattle, preferably for Korean cattle. Recently, the use of animal feed as a cow's feed has been restricted due to concern about mad cow disease, in order to lower the risk for mad cow disease, as the present invention, the feed containing the Cordyceps sinensis cultivated on the basis of vegetable raw materials can be used for Hanwoo Can be. At this time, the cattle using the feed is preferably beef cattle after fattening.

On the other hand, the present invention also provides a method of rearing the ruminant shaft using the feed for the ruminant shaft of the present invention. At this time, the feed containing the Cordyceps sinensis cultivated by the method of the present invention may be fed regardless of the time of breeding, but in consideration of the economical efficiency of breeding, can be fed including Cordyceps in the general feed from 10 weeks before slaughter.

In addition, the content of Cordyceps sinensis contained in the feed may be included in the appropriate ratio in consideration of the feeding time and the age of the ruminant, slaughter time and the like. Preferably, from 10 weeks before slaughter, the content of Cordyceps sinensis in the feed may be 0.1 to 3% by weight for economical breeding.

At this time, when the start of feed of the feed including cordyceps is less than 10 weeks from the slaughter time, the feed start time and the slaughter time are freely considered in the range exceeding 3 wt% of the content of cordyceps in the feed. You can adjust your salary. The higher the content of cordyceps in the feed, the higher the content of cordycepin accumulated in the body.If the feeding period is less than 10 weeks remaining at the time of slaughter, it is preferable to feed with feed containing more than 3% by weight of cordyceps. It can be expected that the content of cordycepin can be used as a functional meat or a functional meat or milk processed product.

Therefore, the meat of the slaughtered ruminant after being bred by the above breeding method has a cordycepin content in the muscle in the range of 0.5 to 4 μg per 1 g of dry meat. In addition, the activity of glutathione peroxidase (GSH-Px) in the plasma of the ruminant axis is in the range of 15 to 30 units (amount of 1 nanomole of oxidized NADPH / min. / Mg of protein). In addition, the content of cordycepin in plasma is in the range of 100 to 600 ng / mL, and thus the content of cordycepin in the muscle of the ruminant shaft is high, and thus it can be widely applied to the food and food industry as a functional well-being meat.

In addition, the meat of the ruminant shaft having a high content of cordycepin as described above may be provided in the form of various processed meat products, and the form is not limited in form if it is commercially available such as ham, sausage, patty. Preferably, the processed meat product has a content of cordycepin per 0.5 g of raw dry meat. By ingesting processed meat products containing cordycepin at the same content as described above, it is possible to expect various effects already described above.

In addition, the milk (milk) produced from the ruminant shaft bred by the breeding method described above of the present invention can be used in various dairy products, etc., the form can also be applied without limitation in the form if it is commercially available milk, cheese, ice cream, etc. have. It is preferable that the raw material dried meat of the ruminant shaft which produced the milk (milk) which is a raw material of the said dairy product contains 0.5-4 microgram of cordycepin per 1g. As described above, the dairy product of the ruminant shaft containing cordycepin is also expected to contain cordycepin, and through its ingestion, various effects already described above can be expected.

The meat of the ruminant shaft, which was reared using the ruminant herb feed containing the cordyceps, according to the method of cultivating the cordyceps of the present invention, has high content of cordycepin in muscle and plasma, high activity of glutathione peroxidase in plasma, and at the same time a vegetable raw material. It is raised as a feed containing Cordyceps sinensis cultivated as a host and has low concern of mad cow disease, and can be widely used in the food and food industry as a functional well-being food.

Hereinafter, the configuration and effect of the present invention through the specific embodiments of the present invention will be described in more detail. However, the following examples are only intended to more clearly understand the present invention, and the present invention is not limited thereto.

[Example]

1. Preparation of feed for Korean cattle containing Cordyceps mycelia

Mycelial strains of Militaris cordyceps grown on isolated soybean protein (ISP, isolated soybean protein; 50% by weight of vegetable-based raw materials) and corn gluten (50% by weight of vegetable-based raw materials) are commonly used as feed. The feed (Nonghyup feed) was added up to 1%, 2%, and 3% by weight based on the total weight to prepare a feed (feeds of Examples 1, 2, and 3, respectively).

2. Breeding of late fattening beef cattle using feed prepared by 1

The random weight of Korean cattle in Okcheon, Chungcheongbuk-do was 20 out of 20 late-rated castrated beef cattle with similar weights (weight range 565 to 574 kg, average weight 569 kg, standard deviation 19.5 kg) and growth age (20 to 24 months). The animals were selected and placed at five heads per pen and bred using the feed according to Examples 1-3. Feeds according to Examples 1 to 3 were fed and reared for 8 weeks in late-casting castrated cattle, and after breeding was completed, slaughtered at Nonghyup Livestock Products Market.

[Comparative Example]

1. Preparation of feed for Korean cattle containing Cordyceps mycelia

The feed of the comparative example consisting of 100% of conventional feed (non-larval feed, Nonghyup feed) without Cordyceps mycelia was prepared.

2. Breeding of late fattening beef cattle using feed prepared by 1

The weight of Korean cattle in Okcheon, Chungcheongbuk-do was 1 out of 20 two of the late-fed castrated Hanwoo cattle with similar weights (weight range 565 to 574 kg, average weight 569 kg, standard deviation 19.5 kg) and growth age (20 to 24 months). The feed of the comparative example was selected and fed in the same manner as in the examples to breed.

After the slaughter, 20 Korean cattle were bred using the feeds of Examples and Comparative Examples, respectively, and the analysis of cordycepin content in muscle and blood and GSH-Px activity, an antioxidant enzyme in plasma, were performed as follows.

[Experimental Example]

Blood and muscle cordycepin assay and plasma GSH-Px activity assay

After feeding with the feeds of the Examples and Comparative Examples, blood was collected through the jugular vein at the 4th week, lyophilized, collected at the 8th week after the feeding, and the slaughtered blood and muscle were cordycepin. Lyophilized to analyze the content and activity of glutathione peroxidase.

1. Analysis of cordycepin content in blood and muscle

Lyophilized blood and muscles were ground to analyze cordycepin. The analysis of cordycepin is as follows. Blood and muscle cordycepin were analyzed by Cunningham et al. (1951). Cordyceps mycelia were extracted in boiling water at 90 ° C. for 6 hours, and after filtration, acetone (v / w) of 2 times by volume based on the mass of the mycelium was added thereto and left at 4 ° C. for 24 hours. After centrifugation at 3,000 rpm for 10 minutes, only the supernatant was taken up and the solvent was completely evaporated using a Rotavapor R-114, Buchi, Switzerland, and dissolved in distilled water, followed by 0.45 μm membrane filter. Filtered by and analyzed by HPLC. The column was used as a WaterBand (USA) μBondapak C ₁₈ column (300 mm L x 3.9 mm ID), the detector was a JASCO UV detector (260 nm). The cordycepin content was calculated from the standard line created by the standard product (No. C3394; C ₁₀ H ₁₃ N ₅ O ₃ ; MW 251.2) purchased from Sigma Co., Ltd. using the peak area indicated on the detector. Figure 2 is a chart comparing the cordycepin concentrations in the blood of Hanwoo blood collected after feeding for 4 weeks and 8 weeks, respectively, according to the Examples and Comparative Examples.

2. Analysis of glutathione peroxidase (GSH-Px) activity in plasma

GSH-Px activity in plasma was measured using specene photometer (Shimrozu, Shimadzu, Japan) 340 using the method of Lawrence and Burk (1976) using cumene hydroperoxide and hydrogen peroxide as enzyme-activated substrates. Activity was measured using the slopes read at intervals of 10 minutes at ㎚ for 3 minutes, and plasma proteins were quantified according to the method of Lowry et al. (1951) in order to express enzymes in protein units.

3 is a chart comparing the activity of glutathione peroxidase (GSH-Px) in plasma of Hanwoo, which was collected after 4 and 8 weeks of feeding according to the Examples and Comparative Examples, respectively.

The concentrations of cordycepin and glutathion peroxidase content of muscle and blood of Hanwoo bred in the feed according to the Examples and Comparative Examples are shown in Table 1.

　
　
Blood cordycepin concentration (ng / mL)

GSH-Px in plasma (unit ² )
Blood collection period 4 Weeks 8 Weeks 4 Weeks 8 Weeks Example 1 137.83 ^b 177.54 ^b 18.46 ^ab 21.11 ^ab Example 2 387.59 ^a 428.84 ^a 20.85 ^ab 23.44 ^a Example 3 397.02 ^a 487.08 ^a 24.71 ^a 26.60 ^a Comparative example Not detected Not detected 15.56 ^b 14.02 ^b Standard error 56.09 59.82 3.5 3.1 valence *** *** * *

¹ statistical significance (*, ***: significant differences at 95 and 99.9% confidence levels, respectively); ² One unit of GSH-Px activity is 1 nanomole of oxidized NADPH / min. / Mg of protein; The average values of treatments with different characters in the same line as a and b differ significantly from each other.

As can be seen in Table 1 and Figures 2 and 3, as a result of varying the content ratio of the plant millitaris cordyceps mycelium in the feed, as the addition amount of Cordyceps mycelia in the feed increased the concentration of cordycepin in Hanwoo significantly It appeared to increase. In addition, the feed group bred with the feed of Examples 2, 3 showed much higher concentrations of cordycepin in blood than the no-feed group bred with the feed of Comparative Example and the feed group bred with the feed of Example 1. However, there was no significant difference in blood cordycepin concentrations between the feed groups bred as feeds of Example 2 and Example 3, and it was found that the feed containing 2 wt% Cordyceps sinensis of Example 2 was more economical.

From the results, it can be seen that a large amount of cordycepin present in Militaris cordyceps is transmitted to the muscle tissue through the blood, and it can be seen that the production of cordycepin-enhanced Hanwoo meat by feeding the vegetable host Cordyceps mycelia. Plasma glutathione peroxidase (GSH-Px) activity showed a similar tendency as the concentration of cordycepin in blood, and especially, the feed-feeding groups of Examples 2 and 3, which were refrained at 8 weeks for feed containing Cordyceps sinensis, were not added to the comparison group. Compared to the two pay groups, there was no significant difference between the two groups. This suggests the efficacy of the metastasis of cordycepin in the Korean cattle's body in Militaris cordyceps mycelium, which is expected to be beneficial to the health of the cattle.

On the other hand, Table 2 shows the content of cordycepin in muscle and liver of the tissues of Hanwoo raised in the feed of the Example and Comparative Example, it is shown in Figure 4 with a chart.

Slaughtered cattle after eight weeks of pay

Muscle (Fuji Meat), [μg / dry g]

Liver, [μg / g dry] Example 1 0.73 ^b 1.17 ^b Example 2 1.82 ^a 2.85 ^a Example 3 2.66 ^a 3.89 ^a Comparative example Not detected Not detected Standard error 0.45 0.56 valence *** ***

¹ statistical significance (***: significant difference at 99.9% confidence level); The average values of treatments with different characters in the same line ^{a, b} differ significantly from each other.

As shown in Table 2 and FIG. 4, the amount of cordycepin in the tissues of Hanwoo cattle was significantly increased (P <0.001) as the amount of dietary fungal mycelial fungus mycelia increased (P <0.001). There was no significant difference between the groups of diets. In particular, the content of cordycepin in muscle and liver up to 2.66 µg and 3.89 µg per g, respectively, can be used in a variety of industrial applications and foods as functional cordycepin fortified beef.

1 is a photograph taken directly of the culture process of Mycelialis Cordyceps mycelia.

2 is a chart comparing the concentrations of cordycepin in blood taken after slaughter of Hanwoo fed the feeds of Examples and Comparative Examples.

FIG. 3 is a chart comparing the activity of glutathione peroxidase (GSH-Px) in plasma collected after slaughter of Hanwoo fed the feed of the Examples and Comparative Examples.

Figure 4 is a chart comparing the content of cordycepin contained in the tissues (Fuji meat and liver) after the slaughter of the feed of the feeds of the Examples and Comparative Examples.

Claims (15)

Preparing a Cordyceps sinensis growth medium containing 25 to 30 wt% of a Cordyceps cultivation composition containing a vegetable raw material having a protein content of 50 wt% or more, and a water content of 70 to 75 wt%; Inoculating the liquid seed of Cordyceps sinensis on the Cordyceps sinensis growth medium; Culturing the medium inoculated with the liquid spawn to form and grow mycelia and fruiting bodies of Cordyceps sinensis; And The herbaceous and fruiting bodies formed and grown in a medium for the ruminant feed containing cordyceps grown in a method comprising the step of harvesting the cordyceps in the grown medium, the semi-stock livestock having a cordycepin content in the muscle of 0.5 to 4ug per 1g of dry meat Meat. According to claim 1, Formation and growth of the mycelium and fruiting body is the mycelium of Cordyceps sinensis by culturing the medium inoculated with the liquid spawn of Cordyceps sinensis for 20 to 30 days at a temperature of 20 to 25 ℃ and a roughness of 500 to 1200 LUX Forming and growing; And A medium axis comprising the step of forming and growing the fruiting body of Cordyceps sinensis by incubating the cultured mycelium-forming medium for 30 to 40 days at a temperature of 19 to 25 ° C, a roughness of 500 to 1200 LUX, and a humidity of 85 to 95%. Meat of cattle. The method of claim 1, Prepared Cordyceps Sinensis medium is semi-axial livestock meat which undergoes sterilization. delete The method of claim 1, The Cordyceps sinensis meat of semi-axial livestock is contained in the ratio of 0.1 to 3% by weight in the feed. The method of claim 1, The cordyceps is meat of semi-axial livestock which is a mycelium of Militaris cordyceps. The method according to claim 1, The feed is meat of semi-axial livestock for Hanwoo. delete The method of claim 1, A ruminant is meat of a semi-animal, which is a carcass. The method of claim 1, Meat of bred ruminant cattle fed 0.1 to 3% by weight of Cordyceps sinensis in the feed 10 weeks before slaughter. delete The method of claim 1, The glutathione peroxidase (GSH-Px) activity in the plasma of the ruminant axis is 15 to 30 units (1 nanomole of oxidized NADPH amount / min. / Protein mg) of the ruminant shaft. The method of claim 1, Meat of the ruminant axis having cordycepin content of 100-600 ng / mL in plasma of the ruminant axis. A meat product comprising the meat of a ruminant shaft, wherein the cordycepin content of claim 1 is 0.5 to 4 μg per 1 g of dry meat. A dairy product comprising the meat of the ruminant shaft, wherein the cordycepin content of claim 1 is 0.5 to 4 μg per 1 g of dry meat.

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