KR20050118416A - Composition for cultivating cordyceps and cultivating process using thereof - Google Patents

Abstract

The present invention relates to a composition for cultivating Cordyceps sinensis and a cultivation method using the same, and particularly, to maximize the efficacy of Cordyceps sinensis, and to cultivate the Cordyceps sinus odorless and odorless to cultivate the composition of the Cordyceps sinensis It is about.

The composition for cultivating Cordyceps sinensis according to the present invention is characterized by containing an effective amount of one substance or a mixture of two or more selected from the group consisting of gluten, soy protein and brewer's yeast.

Description

Cultivation composition of Cordyceps sinensis and cultivation method using same {COMPOSITION FOR CULTIVATING CORDYCEPS AND CULTIVATING PROCESS USING THEREOF}

The present invention relates to a composition for cultivating Cordyceps sinensis and a cultivation method using the same, and particularly, to maximize the efficacy of Cordyceps sinensis, and to cultivate the Cordyceps sinus odorless and odorless to cultivate the composition of the Cordyceps sinensis It is about.

Cordyceps sinensis strain, ie, Cordyceps sinensis, belongs to the genus Aspergillus ganglia, erectile fungi , and is a type of insect parasite ( Entomopathogenic fungi ) that invades insects and forms fruiting bodies or spores on the hosts. The name Cordyceps sinensis (冬蟲夏草) is the name of the insect that enters the body of the insect in the winter to absorb insects and kills the insect, and in the summer, it is made from the body of dead insects. Cordyceps sinensis, which is a kind of Cordyceps sinensis , is used as a name for the fruiting body including mycelium generated by parasitism from various fungi.

More specifically, Cordyceps militaris (aka, " Militaris Cordyceps", hereinafter), Cordyceps scarabaeicola , Cordyceps specose , etc. Palazzo and things like (Cordyceps sphecocephala), coordination sepseu tree Sentry (Cordyceps tricentri) and coordinated sepseu sinen sheath (Cordyceps sinensis), that in addition there are Cordyceps analogs such as one people Paecilomyces japonica (Paecilomyces japonica).

Cordyceps sinensis has long been known as one of China's three rare medicines, along with ginseng and antler, especially for various diseases such as cold, pulmonary tuberculosis, chronic cough, asthma, seizures, anemia, jaundice, hypogonadism and hypertension in men. It is known to be effective. This effect is due in particular to components such as Cordycepin (3-deoxyadenosine) contained in the fruiting bodies or mycelium of Cordyceps sinensis, which enhances immunity, protects the lungs and strengthens the kidneys. Has pharmacological effects In other words, as a result of continuous research, the fruiting body of Cordyceps sinensis has been found to contain various pharmacological components such as cordycepin, and interest in Cordyceps was further increased (Cunningham. KG, Hutchinso. SA, Manson. W., and Spring.FS 1951.Cordycepin, a metabolic product from cultures of Cordyceps militaris (Linn) Link.Pt. 1. isolation and Characteristics, J. Chem. Soc. 51: 2290-2300).

On the other hand, Korea has been successful in the artificial cultivation of Cordyceps sinensis for several years, in particular, silkworms or chrysalis, using the brown rice as a raw material for planting snowflower Cordyceps and Militaris Cordyceps. However, among them, Snow Cordyceps sinensis substantially does not contain cordycepin, so it does not exhibit the pharmacological effects peculiar to Cordyceps sinensis, and thus is not classified as Cordyceps sinensis in foreign countries, but is included in many Cordyceps related products only in Korea. It is known as having a unique effect of Cordyceps sinensis.

In contrast, Militaris cordyceps and cordyceps sinensis contain a large amount of cordycepin in fruiting bodies and mycelium, which are the main components that exhibit the pharmacological activity of cordyceps, compared to other types of cordyceps. It is known that it can exhibit more excellent efficacy. However, this type of cordyceps is very difficult to collect in the natural state, and the supply is limited, and even in a country where natural harvesting is possible, as the protection of the environment becomes harder and natural harvesting becomes difficult, It is true that Cordyceps sinensis, which exhibits such pharmacological effects, could not be stably supplied.

By the way, according to the conventional cordyceps cultivation method, silkworms or pupa and artificially cultivating cordyceps, which exhibits great pharmacological effects, such as the Militaris cordyceps or cordyceps sinensis, by using brown rice, collected in a natural state In contrast, the content of cordycepin contained in the fruiting body or mycelium is very small, there was a problem that can not properly exhibit the unique pharmacological efficacy of these kinds of Cordyceps sinensis.

In addition, the silkworm or pupa used as a host in the past has an unpleasant odor, and there was also a problem that the Cordyceps cultivated by using the cordyceps was sensually severely rejected to be used in various foods and beverages.

That is, in order to obtain a predetermined pharmacological efficacy by using Cordyceps sinensis artificially cultivated according to the prior art, a certain amount or more should be presumed because they contain only a small amount of cordycepin compared to the natural state, but from pupa or silkworm Due to the characteristic unpleasant odor, the amount of usage could not be increased as it is in the final cultivated state. Therefore, in order to include it in food and beverages while maintaining a certain amount of use of the Cordyceps sinensis, various additives are inevitably combined. Since it had to be used, it became a factor which further raised the cost of various foods and drinks including Cordyceps sinensis.

Due to the problems of the prior art, it is possible to further increase the content of cordycepin contained in the fruiting body or mycelium of Cordyceps sinensis which exhibits great pharmacological efficacy, such as the Militaris cordyceps or Cordyceps sinensis, and at the same time, the final cultivated Cordyceps There is an urgent need for a composition or method for cultivating Cordyceps sinensis which can completely remove the unpleasant odor of

In order to solve the problems of the prior art as described above, by increasing the content of cordycepin contained in Cordyceps sinensis to maximize the efficacy of the Cordyceps sinensis, Cordyceps sinensis to be cultivated tasteless and odorless at the same time To provide a composition for cultivation and a method of cultivation thereof.

To achieve this object, the present invention contains an effective amount of one substance or a mixture of two or more selected from the group consisting of gluten, soy protein, Corn Steep Liquor (CSL), or brewer's yeast. It provides a composition for cultivating Cordyceps sinensis, characterized in that.

As apparent from the following examples, when the cordyceps is cultivated using the cultivation composition of the present invention containing an effective amount of gluten, soy protein or brewer's yeast, the cordyceps cultivated in silkworm or pupa according to the prior art In comparison, since the content of cordycepin contained in the mycelium and fruiting body is greatly increased, the pharmacological efficacy of the final cultivated Cordyceps sinensis may be greatly increased. In addition, by cultivating Cordyceps sinensis using the composition of the present invention, such as gluten, soy protein, CSL or brewer's yeast, the unpleasant odor, which is a problem in the prior art, can be completely removed.

In the present invention, Cordyceps sinensis encompasses all Cordyceps sinensis known to exert predetermined pharmacological efficacy by containing a large amount of cordycepin in fruiting bodies or mycelium, such as Militaris cordyceps or Cordyceps sinensis. However, Cordyceps sinensis analogues, for example, Cordyceps sinensis, which does not substantially contain cordycepin and cannot produce the peculiar efficacy of Cordyceps sinensis, do not fall within the scope of Cordyceps sinus in the present invention.

On the other hand, in the composition for cultivating Cordyceps sinensis according to the present invention, it is preferable to use isolated soy protein, tissue soy protein, alpha soy protein, or soybean protein finished product of Beziland.

Soy protein refers to a protein component extracted from soybean belonging to the legume (Leguminosae), in particular, soybean extract, as it is known that contains a variety of biologically active ingredients such as isoflavones Ingredients, in particular the soy protein, have conventionally been used in various high protein foods or health foods. However, in the prior art, there has been no attempt to use such a soy protein in the cultivation composition of Cordyceps sinensis.

However, the present inventors cultivated Cordyceps sinensis with the composition of the present invention containing such a soy protein, the fruit body of the final cultivated Cordyceps sinensis by the action of a high content of protein, other physiologically active ingredients and nutritional components contained in soy protein Or the content of cordycepin contained in the mycelium, etc. has been greatly increased, moreover, soybean protein is a material that has been used conventionally in various foods, etc., the final cultivated Cordyceps sinensis does not give any unpleasant smell at all.

Of these soy proteins, isolated soy protein refers to the most refined soy protein products, which contain protein components in a high content of 90% or more, and tissue soy protein gives good texture to the conventional soy protein and is easy to eat The palatability as is improved. In addition, the alpha (α-ized) soy protein is applied to the soy protein, such as water and heat, such that the soy protein is an α-protein having an irregular molecular structure (usually α-protein is absorbed into the living body) Lastly, Veggieland's finished soy protein is a food additive prepared by mixing soy concentrate and tissue soy protein for use as a meat substitute, and a-izing it. Compared with soy protein products, the protein contains a higher amount of protein that can be easily absorbed, and also has the characteristics of tissue soy protein with excellent palatability.

That is, the isolated soy protein, tissue soy protein, alpha-ized soy protein, and Veggieland's soy protein finished product contain higher protein among various soy protein products, and at the same time, nutrients such as these proteins are absorbed by cordyceps. It is contained in an easy form and processed into a form suitable for ingestion as a food, and when Cordyceps is cultivated using a composition containing such an active ingredient, the content of cordycepin contained in the fruiting body or mycelium of the final cultivated Cordyceps is more. At the same time, it is possible to grow Cordyceps in such a state that the rejection due to an unpleasant odor or taste in ingestion of Cordyceps is minimized.

In the composition for cultivating Cordyceps sinensis according to the present invention, it is preferable to use corn gluten or wheat gluten as the gluten.

Here, gluten refers to a mixture of two simple proteins, such as glutenine and prolamin, and has been used as various food additives in the past and can be obtained from corn flour or flour. In particular, those obtained from corn flour are referred to as corn gluten, and those obtained from wheat flour are referred to as wheat gluten.

As a result of the experiment of the present inventors, even when cultivating cordyceps using such a composition comprising corn gluten and wheat gluten, due to the high protein content contained in the gluten, cordycepin compared to cordyceps grown by the conventional method It has been found that the content of can be greatly increased. In addition, gluten, such as wheat gluten or corn gluten, as a substance that has conventionally been used as a food additive, it is possible to ensure that the final cultivated Cordyceps sinensis do not give an unpleasant smell.

On the other hand, the composition for cultivation according to the present invention may include not only the gluten or soy protein, but also brewer's yeast, such brewer's yeast is used for fermenting the wort in the process of making beer by fermenting wort cooked malt Since the brewer's yeast is isolated after absorbing many nutrients from wort after the final production of beer, the final brewer's brewer's yeast contains a large amount of protein, vitamins, essential minerals, plant fibers and nucleic acids. It contains nutrients, and thus has been conventionally used in many health foods and the like.

That is, due to the large amount of nutrients, such as protein contained in the brewer's yeast, when the cordyceps is cultivated with a composition comprising the same, the content of cordycepin in the final cultivated cordyceps can be greatly increased, and the brewer's yeast is also conventionally Since it is a substance used in health foods, etc. can completely remove the unpleasant smell of Cordyceps sinensis, which appeared in the prior art.

In addition, in the cultivation composition of the present invention, CSL, that is, concentrated corn steep liquor may also be included as an active ingredient, the CSL is a kind of by-product generated in the process of manufacturing processed foods using corn. it means. In general, in processing corn, corn is immersed in sulfurous water before processing. In this process, the nutrient content of corn is eluted, so that the by-product of this process also contains a large amount of nutrients including protein. do. This by-product is CSL, which has a high protein content and contains a large amount of other nutrients, and thus can be included as an active ingredient in the composition of the present invention for the cultivation of Cordyceps sinensis.

However, as clearly demonstrated through the following examples, when cultivating Cordyceps sinensis with the cultivation composition comprising the CSL, the composition comprising other active ingredients of the present invention, that is, soy protein, gluten or brewer's yeast The growth of Cordyceps sinensis is slower than that of cultivation, and the content of cordycepin contained in Cordyceps is lowered. Nevertheless, cultivating Cordyceps sinensis with a cultivating composition containing CSL can solve the problem of unpleasant odor of Cordyceps sinus in the prior art, and thus can include a large amount of Cordyceps sinensis without using a separate additive. .

In addition, the CSL has been conventionally used as a raw material for the fermentation medium for pharmaceuticals, so it can be sufficiently secured at low cost, and cultivation of Cordyceps sinensis with the composition of the present invention comprising the same can greatly reduce the cost of planting Cordyceps sinensis, For this reason, it can be used preferably for cultivating Cordyceps sinensis used for feed.

On the other hand, the composition for cultivating Cordyceps sinensis according to the present invention is prepared by mixing the components of gluten, soy protein, CSL or brewer's yeast with water, the components of the gluten, soy protein, CSL or brewer's yeast are the whole composition It is preferably included in the content of 20-50% by weight based on the weight of, with the remaining content, that is, 50-80% by weight of water.

If the content of the components such as gluten, soy protein, CSL or brewer's yeast is less than 20% by weight, there is a disadvantage that the nutrients, such as proteins necessary for the cultivation of Cordyceps, may not be sufficient, and if more than 50% by weight Since the effect of increasing the yield of Cordyceps sinensis due to the increase in the content of the component can not be achieved, it can be a factor of the increase in the cost of cultivation for Cordyceps sinensis.

However, the cultivation composition of the present invention, in addition to the active ingredients such as gluten, soy protein, CSL or brewer's yeast and other substances containing nutrients, such as proteins required for the cultivation of Cordyceps sinensis, and helps in activating Cordyceps cultivation Other materials and the like, which can be apparent to those skilled in the art, can be included obviously, and the present invention can not be construed as limiting the addition of such components.

For example, in addition to the cultivating composition of the present invention, a pupa or silkworm, which is conventionally used, may be included in a small amount within a range that does not cause an unpleasant smell or taste, and such a composition also belongs to the scope of the present invention. It can be interpreted as falling within the scope of the present invention as long as it contains the main active ingredient.

On the other hand, the present invention further comprises the steps of preparing a Cordyceps sect. Growth medium comprising the composition for cultivating Cordyceps sect. According to the present invention, sterilizing the Cordyceps sect. Growth medium, and liquid seedlings of Cordyceps sect. Cultivating Cordyceps sinensis comprising the step of inoculating, culturing the medium inoculated with the liquid spawn to form and grow mycelia and fruiting bodies of Cordyceps sinensis, and harvesting Cordyceps sinensis in the medium in which the mycelium and fruiting bodies are formed and grown. Provide a method.

That is, the cultivation method of Cordyceps sinensis is characterized by cultivating Cordyceps sinensis through suitable conditions and procedures for the composition, while using the composition for cultivating Cordyceps sinensis according to the present invention as described above. By cultivating Cordyceps sinensis through the method, can be harvested Cordyceps sinensis with significantly increased content of cordycepin, at the same time can completely eliminate the unpleasant smell or taste of the final cultivation Cordyceps sinensis, conditions of cultivation Cordyceps sinensis using the composition of the present invention By optimizing the yield of Cordyceps sinensis can also be greatly increased.

In the method for cultivating Cordyceps sinensis according to the present invention, the formation and growth of the mycelium and fruiting bodies are carried out in a medium in which the liquid seedlings of Cordyceps sinensis are inoculated at a temperature of 20-25 ° C. and a roughness of 500-1200 LUX. Cultured for 1 day to form and grow mycelium of Cordyceps sinensis, and the medium in which the mycelium was formed and grown for 30-40 days at a temperature of 19-25 ° C., a roughness of 500-1200 LUX and a humidity of 85-95%. It is preferred to proceed by culturing to form and grow fruiting bodies of Cordyceps sinensis.

It specifies conditions optimized for the most vigorous formation and growth of the fruiting body and mycelium of Cordyceps sinensis. By cultivating Cordyceps under these conditions, the harvest time of the Cordyceps is reduced, the yield is increased, and the content of cordycepin contained in the fruiting body and mycelium. It can contribute greatly to increase.

In addition, in the cultivation method of Cordyceps sinensis, the sterilization step is preferably carried out by autoclaving for 30-90 minutes at a temperature of 100-135 ℃. This is a condition for the most complete sterilization of the medium. If the sterilization step is carried out under such conditions, all bacteria and the like which are disadvantageous for the cultivation of cordyceps contained in the medium for cordyceps growth can be removed. In other words, by proceeding the subsequent cultivation process in a sterile medium in this way, it is possible to completely prevent the problem that the Cordyceps is contaminated with bacteria or the growth of Cordyceps is suppressed due to such bacteria, so as to improve the quality of the Cordyceps and increase the yield It can contribute greatly.

On the other hand, the inoculation step of the liquid spawn proceeding after the sterilization step is inoculated with the Cordyceps fungi in a flat medium, etc. according to a conventional method known to those skilled in the art, and incubated in a constant temperature period for a period of time to make an inoculator, shaking the inoculator After the proliferation process for a period of time, the resultant can be proceeded by homogenizing the resultant homogenizer (homogenizer) to the final production of the liquid seed of Cordyceps sinensis, and inoculating it into the sterile Cordyceps growth medium.

As described above, cultivating Cordyceps sinensis through the cultivation composition and cultivation method of the present invention can greatly increase the content of cordycepin in Cordyceps so as to maximize the pharmacological effects peculiar to Cordyceps sinensis, and the unpleasant odor of Cordyceps sinensis which appeared in the prior art. The problem can be completely eliminated, and also, by optimizing the cultivation conditions of Cordyceps sinensis, the yield of such Cordyceps can also be increased.

Hereinafter, the configuration and operation of the present invention through a preferred embodiment of the present invention will be described in more detail. However, the scope of the present invention is not defined by these embodiments, but it is presented as an example.

Example 1 Preparation of Cultivated Composition of Cordyceps Sinensis (Including Single Active Ingredient)

One of the active ingredients as shown in Table 1 and water was mixed to prepare a composition for cultivating Cordyceps sinensis. At this time, the content of the active ingredient was 20-50% by weight, and water was 50-80% by weight.

On the other hand, after cultivating Cordyceps sinensis through such a cultivation composition and the cultivation method according to Example 3, by measuring the content of cordycepin, growth rate, smell and taste of mycelia, cultivation cost of Cordyceps sinensis The results are shown together in Table 1 below.

However, in Examples 2, 3, and Comparative Example 1 described below, the results of cultivation of Militaris cordyceps, which is known to show the greatest pharmacological effect among the various cordyceps, was found to have the highest content of cordycepin. Representative examples are shown.

TABLE 1

Active ingredient Mycelial Growth Rate Cordycepin amount Already Off-flavor price Food Universal Feedability 1. Corn Gluten ++++ ++ ++ +++ ++++ +++ ++++ 2. Wheat Gluten ++++ +++ ++++ ++ +++ +++ +++ 3. Soy Protein Isolate ++++ ++++ +++ ++++ - ++++ - 4. Brewer's Yeast +++ ++ ++++ ++++ - +++ - 5. Tissue Soy Protein (Defatted Soybean, Concentrated Soy Protein) ++++ +++ +++ +++ ++ +++ - 6. CSL + + - - ++++ - - 7. Veggieland Soya Protein Complete +++ ++++ ++++ ++++ - ++++ -

* The results of each experiment and measurement indicated the appropriate degree by the number of "+" and the inappropriate degree by the number of "-". In addition, the number of "+" and the number of "-" in each item are reported as 4 and 2 maximums (ie, "++++" is the most suitable for the item, and the most unsuitable is The number of "+" and "-" is indicated according to the ratio of the goodness and incompatibility of each composition based on this.

Example 2 Preparation of Cultivated Composition of Cordyceps Sinensis (Containing Two or More Active Ingredients)

Each active ingredient and water as shown in Table 2 were mixed to prepare a composition for cultivating Cordyceps sinensis. At this time, the total content of the active ingredient included in one cultivation composition was 20-50% by weight, and water was 50-80% by weight.

On the other hand, after cultivating Cordyceps sinensis through the composition for cultivating Cordyceps sinensis and the cultivation method according to Example 3 below, the content of cordycepin, growth rate, odor and taste of mycelia, and cultivation cost of Cordyceps sinensis The results are shown together in Table 2 below.

TABLE 2

Active ingredient Mycelial Growth Rate Cordycepin amount Already Off-flavor price Food Universal Feedability 8. 1 + 4 + 7 ++++ ++++ ++++ ++++ - ++++ + 9. 1 + 3 + 4 ++++ ++++ +++ ++++ - ++++ - 10. 1 + 3 +++ ++++ +++ +++ + +++ - 11.2 + 4 + 7 ++++ ++++ ++++ ++++ - ++++ - 12. 2 + 3 + 4 ++++ ++++ +++ ++++ + ++++ - 13. 2 + 3 +++ +++ +++ +++ + +++ - 14. 1 + 4 + 6 + 7 + ++ ++ ++ + + + 15. 1 + 3 + 4 + 6 + ++ ++ ++ + + + 16. 1 + 3 + 6 +++ + + + ++ + + 17.2 + 4 + 6 + 7 +++ ++ ++ ++ + + + 18.2 + 3 + 4 + 6 +++ ++ + ++ + + + 19.2 + 3 + 6 +++ + + ++ ++ + + 20. 1 + 6 + - - + ++++ - - 21.2 + 6 + - - + ++++ - -

* The results of each experiment and measurement indicated the appropriate degree by the number of "+" and the inappropriate degree by the number of "-". In addition, the number of "+" and the number of "-" in each item are reported as 4 and 2 maximums (ie, "++++" is the most suitable for the item, and the most unsuitable is The number of "+" and "-" is indicated according to the ratio of the goodness and incompatibility of each composition based on this.

** Each number described in the active ingredient column of Table 2 means the number of each active ingredient described in Table 1 of Example 1.

Comparative Example 1 Preparation of Host for Caterpillar Cultivation by the Prior Art (including pupa)

Using the pupa as shown in Table 3 below to prepare a host for cultivating Cordyceps sinensis according to the prior art. At this time, since the pupa itself contains about 50% of water, the pupa itself was used as a cultivation host of Cordyceps sinensis without being mixed with water separately.

 On the other hand, after cultivating cordyceps through the conventional cultivation host and the cultivation method according to Example 3 below, the content of cordycepin contained in the cordyceps, growth rate of the mycelium, smell and taste, cost of cultivating cordyceps, etc. It measured and showed the result in Table 3 below.

TABLE 3

Active ingredient Mycelial Growth Rate Cordycepin amount Already Off-flavor price Food Universal Feedability pupa +++ +++ - - + - -

* The results of each experiment and measurement indicated the appropriate degree by the number of "+" and the inappropriate degree by the number of "-". In addition, the number of "+" and the number of "-" in each item are reported as 4 and 2 maximums (ie, "++++" is the most suitable for the item, and the most unsuitable is The number of "+" and "-" is indicated according to the ratio of the goodness and incompatibility of each composition based on this.

Example 3 cultivation method of Cordyceps sinensis

Step 1: Composition of Cordyceps Sinensis Growth Medium

Cordyceps sinensis growth medium was prepared using the composition (or host) for Cordyceps sinensis cultivation of Examples 1 to 2 and Comparative Example 1, respectively. However, since each of the active ingredients described in Examples 1 and 2 has fine powders, in order to prepare each Cordyceps sinensis cultivation composition by mixing each of these active ingredients and water, they were mixed in order from the active ingredients in the fine powder state. When the mixing was completed to some extent it was left to allow sufficient moisture to penetrate into the active ingredient.

Step 2: sterilization

As described above, after the production of Cordyceps sinensis cultivation composition and the preparation of the Cordyceps sinensis growth medium are completed, the sterilized Cordyceps sinensis growth medium is placed in a clean container and sterilized. Sterilization time was autoclaved at 100-135 ° C. for 30-90 minutes to remove bacteria and the like.

Step 3: inoculation and spawn culture

3. 1-Cordyceps fungi were inoculated into PDA plate medium, which was then incubated in a thermostat under the illuminance of 200-500 LUX for 7-15 days to generate inoculum.

3. 2-The inoculum was placed in an Erlenmeyer flask and shaken in a shake incubator, followed by propagation in a 20 liter culture vessel for 6-8 days and homogenized using a homogenizer to produce liquid spawn of Cordyceps sinensis.

Step 4: Inoculation

The medium in which the two sterilization steps were completed was left for one day. The reason for this neglect is that the media can be contaminated with mold when the sterilization of the media is not good, because if it is left for a day, the contamination can be known. After it was confirmed that it was not contaminated with mold, a small amount of the liquid spawn of Cordyceps sinensis produced in step 3 was inoculated into the medium.

Step 5: Mycelial Culture (Formation and Growth of Mycelia)

The medium in which the inoculation step was completed was incubated in the bacteria. At this time, the culture temperature was 20-25 ° C, and the roughness was 500-1200 LUX. After the start of the culture, the mycelia of Cordyceps sinensis began to stretch about 2 days later, and when left for about 20-30 days, the mycelium spreads yellow throughout the medium to form the shape of the feet.

Stage 6: fruiting body growth (forming and growing fruiting body)

After culturing for a certain period of time, after the formation and growth of mycelia, the medium is transferred to a growth chamber at 19-25 ° C., and the fruiting body is maintained at 500 to 1200 LUX and humidity at 85 to 95%. Was formed and grown. Once the fruiting bodies were formed, watering was done and sprayed using a fog device in small quantities every day or every two days. At this time, the hood was opened to allow fresh air to be introduced from time to time.

This fruiting stage was for about 30-40 days.

Step 7: harvesting Cordyceps sinensis

After the incubation in the step 5 and 6 was dried it was finally harvested Cordyceps sinensis from the medium.

As clearly demonstrated through Examples 1 to 3, Comparative Example 1 and Tables 1 and 2, when Cordyceps sinensis was cultivated through the cultivation composition and cultivation method according to the present invention, significantly higher than that cultivated according to the prior art. It was found that the content of cordycepin was contained, and in particular, the Cordyceps sinensis cultivated by the present invention contained a higher content of cordycepin in the mycelia than the fruiting bodies.

In addition, the Cordyceps sinensis cultivated by the present invention has a fast growth rate and greatly improves the yield and harvesting speed, and does not have an unpleasant smell or taste, so it can be added to food without additional additives and consumed without objection to the user. Could.

In addition, as a result of examining the possibility of use as a feed of cordyceps, cordyceps cultivated by the prior art used animal pupa or silkworm as a raw material, and thus was unsuitable for feed of herbivores such as cattle or sheep, but according to the present invention. It has been found that the prepared Cordyceps Sinensis can be used as feed for any livestock, including these herbivores.

As described above, according to the present invention, it is possible to greatly increase the content of cordycepin contained in the mycelium or fruiting body of Cordyceps sinensis, maximizing the pharmacological effect of Cordyceps sinensis, and at the same time, the unpleasant taste or smell of Cordyceps sinensis Problems such as all can also be solved, it is possible to add a large amount of Cordyceps sinensis to various foods without using a separate additive.

Therefore, the present invention shows a remarkably excellent effect in terms of ease of use (intake) and efficacy of Cordyceps sinensis as a food, there is an advantage that can be widely used as food as well as food.

Claims (8)

A composition for cultivating Cordyceps sinensis containing an effective amount of one substance or a mixture of two or more selected from the group consisting of gluten, soy protein, CSL or brewer's yeast. According to claim 1, wherein the soy protein is a soybean protein, tissue soy protein, alpha soy protein, or the Cordyceps cultivation composition using the finished soy protein of Veziland. The composition for cultivating Cordyceps sinensis using corn gluten or wheat gluten as the gluten. The composition for cultivating Cordyceps sinensis according to any one of claims 1 to 3, which contains 20 to 50% by weight of the gluten, soy protein or brewer's yeast and the remaining amount of water. Preparing a Cordyceps sinensis growth medium comprising the composition for cultivating Cordyceps sinensis according to any one of claims 1 to 3; Sterilizing the cordyceps growth medium; Inoculating the liquid seed of Cordyceps sinensis on the sterilized Cordyceps sinensis medium; Culturing the medium inoculated with the liquid spawn to form and grow mycelia and fruiting bodies of Cordyceps sinensis; And A method for cultivating Cordyceps sinensis comprising harvesting Cordyceps sinensis in a medium in which the mycelium and fruiting bodies are formed and grown. The method of claim 5, wherein the composition for cultivating Cordyceps sinensis comprises 20 to 50% by weight of gluten, soy protein or brewer's yeast, and the remaining amount of water. The method of claim 5 or 6, wherein the formation and growth of the mycelium and fruiting body is incubated in the medium inoculated with the liquid spawn of Cordyceps sinensis for 20-30 days at a temperature of 20-25 ℃ and a roughness of 500-1200 LUX To form and grow mycelia of Cordyceps sinensis; And Cordyceps sinensis comprising the step of culturing the mycelium formed and grown medium for 30-40 days at a temperature of 19-25 ℃, roughness of 500-1200 LUX and humidity of 85-95% to form and grow fruiting bodies of cordyceps Method of cultivation. The method of cultivating Cordyceps sinensis according to claim 5, wherein the sterilization step is performed by autoclaving at a temperature of 100-135 ° C for 30-90 minutes.